Sed0027875 (gene) Chayote v1

Overview
NameSed0027875
Typegene
OrganismSechium edule (Chayote v1)
DescriptionEndo-1,3(4)-beta-glucanase
LocationLG10: 33105708 .. 33107942 (+)
RNA-Seq ExpressionSed0027875
SyntenySed0027875
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AATTAATAAAAATCCTCTCCAACATTTATTTCTCATCAGCCATGGAAACCCCTAAACCATGGCTGATCTGCGTCTTCTTTCTTCAACTTCTCGCCCTGTTTCCGCCGCCGTCCGCCGCCCAGTTCCCCTTCCCGGAAACCACCTCCACGGCGGTTCCCGACCCTACCAAATTCTTCTCTCCCTCCCTCCTTTCTTCTCCACTTCCCACAAATTCCTTCTTCCAGAACTTCGTCCTCAACAATGGCGACTCGCCGGAATACATCCATCCATATCTCATGAGAATCGCGAATTCTTCGCTCTCTGTTTCATACCCATCTCGAACTTCCAATTCTTCGATTACCCAGTTGCCGGTTTTTCCAGATCTTACCATTTCTGCGTTGAACAGAGCTTCCCCTGTTTCTAACAGAACCCATTTCGTTTCTTCCTTCAGCGATCTCGGTGTTGATTTGGACATTGGGGATTTTAGATTTCACCTCGTTCGTGGAAGTCCGTACTTGACTTTCTCTGTTTTGAAAACTTCCTCTGTTTCGATCTCCACCGGCTATGGCGTTCGCTCCGTTGATTCCTCCGGTGATTCGACGAAACACGTCGTTCGATTGAGCAACGGCCGGAATTGGGTGGTTTACTCCACGTCGGCGATTGGCTTGATAAAATCGAAGAACAATGAGATAATCTCAGGGGGATTTTCCGGCGTGATTCGGGTTGCGGTTCTGCCGGATTCAAAGAGTGAATCGGAGAAGATTCTTGATCTGTACAGAGGATGTTACCCTGTTTCTGGGTTTGCGAAATTGTCTGAAAATTATGGGTTTCAATACAAATGGCAGAAGAAGGGAAGTGGAGACCTTTTGATGTTGGCTCACCCGTTGCACCGGCGAATCCTGCCGGGAAATCAATCTGTTCTTCAAAATCTCCGGTACGGCAGCATCGACGGCGACCTTGTGGGAGTCGTCGGAGACTCATGGGATTTGAATTTCAATCCGATTCCGGTGACTTGGCATTCAATCAACGGGATCAACAACAAATTCTTCCCCGAAATTGTTGCGGCTCTTAAAAAAGACGTTAGCATTTTGAACGTTACAGATCTTTCGTCAACGCCGGCGTCCTATTTCTACGGGAAATTGCTAGCCAGAGCTGCGAGATTGGCTTTGATCGCCGAGGAGGTGAATTTCGCCGGCGACGTCGTTCCGGCGGTGGTGAAGTTCTTGAAGAACGGGATTCAGCCATGGCTGGACGGGAAATTTGCAAAGAATGGGTTTCTGTTTGAACGGAAATGGGCTGGATTGGTGACGAAGAACGGCGCCACAAGTCAAACAGAGGATTTCGGATTCGGAATTTACAACGATCACCATTTTCACCTCGGCTATTACGTGTATTCAATCGCCGTTCTCGCAAAACTCGATCCAAATTGGGGGAATCAATACAAGTCCAAAGCGTACGCTTTGGTTAACGACTACATGAATTTCATACCCAAAACATCCCAATTCTCAATTCCGTTTAGAAACTTCGATTTCTGGAAGCTCCACTCATGGGCCGCCGGATTAACAGAGTTCCCGGACGGCCGGAATCAGGAGAGCACAAGCGAGGCCGTGAATGCGTATTACGCGGCGGCGCTGATGGGTATGGCGTACGGCGACAAGGCGCTCACTGACGCCGCAGCGGCGCTGACGGCGGCGGAGATCGCGGCGGCGCAGACGTGGTGGCATGTGAAGAAAGAGGGCTCGATTTACGACCAGGGATTTGCAGGGGAAAACAGAGTGGTGGGGATTTTGTGGAGTGCGGCGAGGGAGAGCCGGCTTTGGTTTGCGCCGCCGGAGTGGCGGGAGTGCAGAGTCGGAATTCAGGTATTGCCGGTGCTGCCGGTGACGGAGAAGGTGTTTTCCGATGAGGAGTTTGTGAGAGAACTTGTGGAGTGGGTGGCGCCGGCGTTGGAACGGGAAGACGCCGGCGAGGGGTGGAAGGGATTTGCTTATGCGTTGGAAGGGATTTATGATTGGAAAAGTGGGGTTGAAAAGATTAAGAAATTGAAGAAACATGATGATGGAAATTCTCTCAGTAATCTCTTGTGGTGGATTTACAGTCGGCCGGCGGTAAAAGGTTGAAGACAAAGTTTTTTTTTTTCATTTATAATTTGTTTTTGTACTCTTTTGAATTTAAATGATTATTGTATTTATAAAATTTAAAAACTAATATTTAATTTTGCCATACTTTATGGAGTTCGATGCTGGATGGAATTTG

mRNA sequence

AATTAATAAAAATCCTCTCCAACATTTATTTCTCATCAGCCATGGAAACCCCTAAACCATGGCTGATCTGCGTCTTCTTTCTTCAACTTCTCGCCCTGTTTCCGCCGCCGTCCGCCGCCCAGTTCCCCTTCCCGGAAACCACCTCCACGGCGGTTCCCGACCCTACCAAATTCTTCTCTCCCTCCCTCCTTTCTTCTCCACTTCCCACAAATTCCTTCTTCCAGAACTTCGTCCTCAACAATGGCGACTCGCCGGAATACATCCATCCATATCTCATGAGAATCGCGAATTCTTCGCTCTCTGTTTCATACCCATCTCGAACTTCCAATTCTTCGATTACCCAGTTGCCGGTTTTTCCAGATCTTACCATTTCTGCGTTGAACAGAGCTTCCCCTGTTTCTAACAGAACCCATTTCGTTTCTTCCTTCAGCGATCTCGGTGTTGATTTGGACATTGGGGATTTTAGATTTCACCTCGTTCGTGGAAGTCCGTACTTGACTTTCTCTGTTTTGAAAACTTCCTCTGTTTCGATCTCCACCGGCTATGGCGTTCGCTCCGTTGATTCCTCCGGTGATTCGACGAAACACGTCGTTCGATTGAGCAACGGCCGGAATTGGGTGGTTTACTCCACGTCGGCGATTGGCTTGATAAAATCGAAGAACAATGAGATAATCTCAGGGGGATTTTCCGGCGTGATTCGGGTTGCGGTTCTGCCGGATTCAAAGAGTGAATCGGAGAAGATTCTTGATCTGTACAGAGGATGTTACCCTGTTTCTGGGTTTGCGAAATTGTCTGAAAATTATGGGTTTCAATACAAATGGCAGAAGAAGGGAAGTGGAGACCTTTTGATGTTGGCTCACCCGTTGCACCGGCGAATCCTGCCGGGAAATCAATCTGTTCTTCAAAATCTCCGGTACGGCAGCATCGACGGCGACCTTGTGGGAGTCGTCGGAGACTCATGGGATTTGAATTTCAATCCGATTCCGGTGACTTGGCATTCAATCAACGGGATCAACAACAAATTCTTCCCCGAAATTGTTGCGGCTCTTAAAAAAGACGTTAGCATTTTGAACGTTACAGATCTTTCGTCAACGCCGGCGTCCTATTTCTACGGGAAATTGCTAGCCAGAGCTGCGAGATTGGCTTTGATCGCCGAGGAGGTGAATTTCGCCGGCGACGTCGTTCCGGCGGTGGTGAAGTTCTTGAAGAACGGGATTCAGCCATGGCTGGACGGGAAATTTGCAAAGAATGGGTTTCTGTTTGAACGGAAATGGGCTGGATTGGTGACGAAGAACGGCGCCACAAGTCAAACAGAGGATTTCGGATTCGGAATTTACAACGATCACCATTTTCACCTCGGCTATTACGTGTATTCAATCGCCGTTCTCGCAAAACTCGATCCAAATTGGGGGAATCAATACAAGTCCAAAGCGTACGCTTTGGTTAACGACTACATGAATTTCATACCCAAAACATCCCAATTCTCAATTCCGTTTAGAAACTTCGATTTCTGGAAGCTCCACTCATGGGCCGCCGGATTAACAGAGTTCCCGGACGGCCGGAATCAGGAGAGCACAAGCGAGGCCGTGAATGCGTATTACGCGGCGGCGCTGATGGGTATGGCGTACGGCGACAAGGCGCTCACTGACGCCGCAGCGGCGCTGACGGCGGCGGAGATCGCGGCGGCGCAGACGTGGTGGCATGTGAAGAAAGAGGGCTCGATTTACGACCAGGGATTTGCAGGGGAAAACAGAGTGGTGGGGATTTTGTGGAGTGCGGCGAGGGAGAGCCGGCTTTGGTTTGCGCCGCCGGAGTGGCGGGAGTGCAGAGTCGGAATTCAGGTATTGCCGGTGCTGCCGGTGACGGAGAAGGTGTTTTCCGATGAGGAGTTTGTGAGAGAACTTGTGGAGTGGGTGGCGCCGGCGTTGGAACGGGAAGACGCCGGCGAGGGGTGGAAGGGATTTGCTTATGCGTTGGAAGGGATTTATGATTGGAAAAGTGGGGTTGAAAAGATTAAGAAATTGAAGAAACATGATGATGGAAATTCTCTCAGTAATCTCTTGTGGTGGATTTACAGTCGGCCGGCGGTAAAAGGTTGAAGACAAAGTTTTTTTTTTTCATTTATAATTTGTTTTTGTACTCTTTTGAATTTAAATGATTATTGTATTTATAAAATTTAAAAACTAATATTTAATTTTGCCATACTTTATGGAGTTCGATGCTGGATGGAATTTG

Coding sequence (CDS)

ATGGAAACCCCTAAACCATGGCTGATCTGCGTCTTCTTTCTTCAACTTCTCGCCCTGTTTCCGCCGCCGTCCGCCGCCCAGTTCCCCTTCCCGGAAACCACCTCCACGGCGGTTCCCGACCCTACCAAATTCTTCTCTCCCTCCCTCCTTTCTTCTCCACTTCCCACAAATTCCTTCTTCCAGAACTTCGTCCTCAACAATGGCGACTCGCCGGAATACATCCATCCATATCTCATGAGAATCGCGAATTCTTCGCTCTCTGTTTCATACCCATCTCGAACTTCCAATTCTTCGATTACCCAGTTGCCGGTTTTTCCAGATCTTACCATTTCTGCGTTGAACAGAGCTTCCCCTGTTTCTAACAGAACCCATTTCGTTTCTTCCTTCAGCGATCTCGGTGTTGATTTGGACATTGGGGATTTTAGATTTCACCTCGTTCGTGGAAGTCCGTACTTGACTTTCTCTGTTTTGAAAACTTCCTCTGTTTCGATCTCCACCGGCTATGGCGTTCGCTCCGTTGATTCCTCCGGTGATTCGACGAAACACGTCGTTCGATTGAGCAACGGCCGGAATTGGGTGGTTTACTCCACGTCGGCGATTGGCTTGATAAAATCGAAGAACAATGAGATAATCTCAGGGGGATTTTCCGGCGTGATTCGGGTTGCGGTTCTGCCGGATTCAAAGAGTGAATCGGAGAAGATTCTTGATCTGTACAGAGGATGTTACCCTGTTTCTGGGTTTGCGAAATTGTCTGAAAATTATGGGTTTCAATACAAATGGCAGAAGAAGGGAAGTGGAGACCTTTTGATGTTGGCTCACCCGTTGCACCGGCGAATCCTGCCGGGAAATCAATCTGTTCTTCAAAATCTCCGGTACGGCAGCATCGACGGCGACCTTGTGGGAGTCGTCGGAGACTCATGGGATTTGAATTTCAATCCGATTCCGGTGACTTGGCATTCAATCAACGGGATCAACAACAAATTCTTCCCCGAAATTGTTGCGGCTCTTAAAAAAGACGTTAGCATTTTGAACGTTACAGATCTTTCGTCAACGCCGGCGTCCTATTTCTACGGGAAATTGCTAGCCAGAGCTGCGAGATTGGCTTTGATCGCCGAGGAGGTGAATTTCGCCGGCGACGTCGTTCCGGCGGTGGTGAAGTTCTTGAAGAACGGGATTCAGCCATGGCTGGACGGGAAATTTGCAAAGAATGGGTTTCTGTTTGAACGGAAATGGGCTGGATTGGTGACGAAGAACGGCGCCACAAGTCAAACAGAGGATTTCGGATTCGGAATTTACAACGATCACCATTTTCACCTCGGCTATTACGTGTATTCAATCGCCGTTCTCGCAAAACTCGATCCAAATTGGGGGAATCAATACAAGTCCAAAGCGTACGCTTTGGTTAACGACTACATGAATTTCATACCCAAAACATCCCAATTCTCAATTCCGTTTAGAAACTTCGATTTCTGGAAGCTCCACTCATGGGCCGCCGGATTAACAGAGTTCCCGGACGGCCGGAATCAGGAGAGCACAAGCGAGGCCGTGAATGCGTATTACGCGGCGGCGCTGATGGGTATGGCGTACGGCGACAAGGCGCTCACTGACGCCGCAGCGGCGCTGACGGCGGCGGAGATCGCGGCGGCGCAGACGTGGTGGCATGTGAAGAAAGAGGGCTCGATTTACGACCAGGGATTTGCAGGGGAAAACAGAGTGGTGGGGATTTTGTGGAGTGCGGCGAGGGAGAGCCGGCTTTGGTTTGCGCCGCCGGAGTGGCGGGAGTGCAGAGTCGGAATTCAGGTATTGCCGGTGCTGCCGGTGACGGAGAAGGTGTTTTCCGATGAGGAGTTTGTGAGAGAACTTGTGGAGTGGGTGGCGCCGGCGTTGGAACGGGAAGACGCCGGCGAGGGGTGGAAGGGATTTGCTTATGCGTTGGAAGGGATTTATGATTGGAAAAGTGGGGTTGAAAAGATTAAGAAATTGAAGAAACATGATGATGGAAATTCTCTCAGTAATCTCTTGTGGTGGATTTACAGTCGGCCGGCGGTAAAAGGTTGA

Protein sequence

METPKPWLICVFFLQLLALFPPPSAAQFPFPETTSTAVPDPTKFFSPSLLSSPLPTNSFFQNFVLNNGDSPEYIHPYLMRIANSSLSVSYPSRTSNSSITQLPVFPDLTISALNRASPVSNRTHFVSSFSDLGVDLDIGDFRFHLVRGSPYLTFSVLKTSSVSISTGYGVRSVDSSGDSTKHVVRLSNGRNWVVYSTSAIGLIKSKNNEIISGGFSGVIRVAVLPDSKSESEKILDLYRGCYPVSGFAKLSENYGFQYKWQKKGSGDLLMLAHPLHRRILPGNQSVLQNLRYGSIDGDLVGVVGDSWDLNFNPIPVTWHSINGINNKFFPEIVAALKKDVSILNVTDLSSTPASYFYGKLLARAARLALIAEEVNFAGDVVPAVVKFLKNGIQPWLDGKFAKNGFLFERKWAGLVTKNGATSQTEDFGFGIYNDHHFHLGYYVYSIAVLAKLDPNWGNQYKSKAYALVNDYMNFIPKTSQFSIPFRNFDFWKLHSWAAGLTEFPDGRNQESTSEAVNAYYAAALMGMAYGDKALTDAAAALTAAEIAAAQTWWHVKKEGSIYDQGFAGENRVVGILWSAARESRLWFAPPEWRECRVGIQVLPVLPVTEKVFSDEEFVRELVEWVAPALEREDAGEGWKGFAYALEGIYDWKSGVEKIKKLKKHDDGNSLSNLLWWIYSRPAVKG
Homology
BLAST of Sed0027875 vs. NCBI nr
Match: XP_038889504.1 (putative endo-1,3(4)-beta-glucanase 2 [Benincasa hispida])

HSP 1 Score: 1157.5 bits (2993), Expect = 0.0e+00
Identity = 566/689 (82.15%), Postives = 620/689 (89.99%), Query Frame = 0

Query: 1   METPKPWLICV--FFLQLLALFPPPSAAQFPFPETTSTAVPDPTKFFSPSLLSSPLPTNS 60
           METPKPWLI +  FF  +L  FPP  + QF FP+TTSTAVPDP KFFSP+LLSSPLPTNS
Sbjct: 23  METPKPWLISIFFFFFIILTAFPPAVSGQFLFPQTTSTAVPDPAKFFSPTLLSSPLPTNS 82

Query: 61  FFQNFVLNNGDSPEYIHPYLMRIANSSLSVSYPSRTSNSSITQLPVFPDLTISALNRASP 120
           FFQNFVLNNGD PEYIHPY++R ANSSLSVSYPSR SNSSITQL  +PDLTISAL+R SP
Sbjct: 83  FFQNFVLNNGDKPEYIHPYIVRTANSSLSVSYPSRISNSSITQLQFYPDLTISALDRTSP 142

Query: 121 VSNRTHFVSSFSDLGVDLDIGDFRFHLVRGSPYLTFSVLKTSSVSISTGYGVRSVDSSGD 180
           VSN THF+SSFSDLGVDLDIGDFRFHLVRGSPYLTFSVLK SSVSISTG GVRSVDS  D
Sbjct: 143 VSNTTHFISSFSDLGVDLDIGDFRFHLVRGSPYLTFSVLKISSVSISTGNGVRSVDSYDD 202

Query: 181 STKHVVRLSNGRNWVVYSTSAIGLIKSKNNEII-SGGFSGVIRVAVLPDSKSESEKILDL 240
           STKH++RL+NGRNWV+YS+SAI LIKSKNN+I+ SGGFSGVIR+AVLP+S  ES  ILD 
Sbjct: 203 STKHIIRLNNGRNWVLYSSSAIYLIKSKNNQIVTSGGFSGVIRIAVLPNSAMESGNILDR 262

Query: 241 YRGCYPVSGFAKLSENYGFQYKWQKKGSGDLLMLAHPLHRRILPGNQSVLQNLRYGSIDG 300
           Y GCYPVSG+ KLSEN+GF+YKWQKKGSG LLMLAHPLHR+IL  NQ+VL NLRYGSIDG
Sbjct: 263 YSGCYPVSGYVKLSENFGFKYKWQKKGSGGLLMLAHPLHRQILSRNQTVLPNLRYGSIDG 322

Query: 301 DLVGVVGDSWDLNFNPIPVTWHSINGINNKFFPEIVAALKKDVSILNVTDLSSTPASYFY 360
           DL+GVVGDSWDLNFNP+P+TWHSINGI+ KFFPEIVAALK+DV+ LNVT+LSST +SYFY
Sbjct: 323 DLLGVVGDSWDLNFNPVPITWHSINGIDKKFFPEIVAALKRDVATLNVTELSSTASSYFY 382

Query: 361 GKLLARAARLALIAEEVNFAGDVVPAVVKFLKNGIQPWLDGKFAKNGFLFERKWAGLVTK 420
            KLLARAARLALIAEEV+    V+PAVV+FLKNGIQPWL GKF KNGFL+ERKWAGLVTK
Sbjct: 383 AKLLARAARLALIAEEVDNGAGVIPAVVRFLKNGIQPWLIGKFPKNGFLYERKWAGLVTK 442

Query: 421 NGATSQTEDFGFGIYNDHHFHLGYYVYSIAVLAKLDPNWGNQYKSKAYALVNDYMNFIPK 480
           NGATS TEDFGFGIYNDHHFHLGY+VYSIAVLAKLDPNWG Q+K +AYALV DYMNF PK
Sbjct: 443 NGATSTTEDFGFGIYNDHHFHLGYFVYSIAVLAKLDPNWGKQFKPQAYALVYDYMNFRPK 502

Query: 481 TSQFSIPFRNFDFWKLHSWAAGLTEFPDGRNQESTSEAVNAYYAAALMGMAYGDKALTDA 540
            SQFSIPFRNFDFWKLHSWAAGLTEFPDGRNQESTSEAVNAYYAAALMG+AY D++LT A
Sbjct: 503 KSQFSIPFRNFDFWKLHSWAAGLTEFPDGRNQESTSEAVNAYYAAALMGLAYDDESLTAA 562

Query: 541 AAALTAAEIAAAQTWWHVKKEGS-IYDQGFAGENRVVGILWSAARESRLWFAPPEWRECR 600
            + LTAAEIAA QTWWHVK E + IYD+GF  ENRVVGILWSAARESRLWFAP EWRECR
Sbjct: 563 GSTLTAAEIAATQTWWHVKSEKNWIYDKGFEEENRVVGILWSAARESRLWFAPAEWRECR 622

Query: 601 VGIQVLPVLPVTEKVFSDEEFVRELVEWVAPALEREDAGEGWKGFAYALEGIYDWKSGVE 660
           VGIQVLPVLPV+E+VFSD+ FV+E+VEWV+ ALEREDAGEGWKGFAYALEGIYD KS +E
Sbjct: 623 VGIQVLPVLPVSERVFSDKGFVKEVVEWVSRALEREDAGEGWKGFAYALEGIYDKKSAIE 682

Query: 661 KIKKLKKHDDGNSLSNLLWWIYSRPAVKG 686
           K+KKLKKHDDGNSLSNLLWWIYSRP  +G
Sbjct: 683 KVKKLKKHDDGNSLSNLLWWIYSRPEGRG 711

BLAST of Sed0027875 vs. NCBI nr
Match: KAG7025154.1 (hypothetical protein SDJN02_13977, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1136.3 bits (2938), Expect = 0.0e+00
Identity = 563/692 (81.36%), Postives = 613/692 (88.58%), Query Frame = 0

Query: 1   METPKPWLICVFFLQLLALFP---PPSAAQFPFPETTSTAVPDPTKFFSPSLLSSPLPTN 60
           ME P PWL+  F L L A F    PPS+AQFPFPETTSTAVPDPTKFFSPSLLSSPLPTN
Sbjct: 1   MEAPNPWLLPFFLLLLAAAFSDQLPPSSAQFPFPETTSTAVPDPTKFFSPSLLSSPLPTN 60

Query: 61  SFFQNFVLNNGDSPEYIHPYLMRIANSSLSVSYPSRTSNSSITQLPVFPDLTISALNRAS 120
           SFFQNFVLNNGD PEYIHPYL+R ANSSLSVSYPSRTSNSSITQLP  PDLTIS+     
Sbjct: 61  SFFQNFVLNNGDLPEYIHPYLIRTANSSLSVSYPSRTSNSSITQLPFSPDLTISS----- 120

Query: 121 PVSNRTHFVSSFSDLGVDLDIGDFRFHLVRGSPYLTFSVLKTSSVSIST-GYGVRSVDSS 180
            VSN+THFVSSFSDL VDLDIGDFRFHLVRGSPYLTFSVLK SSVSIST G GV SVDS 
Sbjct: 121 -VSNKTHFVSSFSDLAVDLDIGDFRFHLVRGSPYLTFSVLKNSSVSISTGGNGVGSVDSY 180

Query: 181 GDSTKHVVRLSNGRNWVVYSTSAIGLIKSKNNEII-SGGFSGVIRVAVLPDSKSESEKIL 240
            DSTKH++RLSNGR WVVYST+AI L+K K++EI+ SGGFSGVIRVAVLP+S +E+E+IL
Sbjct: 181 DDSTKHIIRLSNGRTWVVYSTAAIYLMKGKSSEIVTSGGFSGVIRVAVLPNSAAETEQIL 240

Query: 241 DLYRGCYPVSGFAKLSENYGFQYKWQKKGSGDLLMLAHPLHRRILPGNQSVLQNLRYGSI 300
           D Y GCYPVSG+AKLS N+GF YKWQKKGSG LLMLAHPLHRR+L  N +VLQN +YGSI
Sbjct: 241 DRYSGCYPVSGYAKLSGNFGFSYKWQKKGSGGLLMLAHPLHRRVLSENLTVLQNFKYGSI 300

Query: 301 DGDLVGVVGDSWDLNFNPIPVTWHSINGINNKFFPEIVAALKKDVSILNVTDLSSTPASY 360
           DGDLVGVVGDSWDLNF PIP+TWHSINGI  KFFPEIVAALK DV  LNVT+LSST ASY
Sbjct: 301 DGDLVGVVGDSWDLNFAPIPITWHSINGIERKFFPEIVAALKHDVGTLNVTELSSTAASY 360

Query: 361 FYGKLLARAARLALIAEEVNFAGDVVPAVVKFLKNGIQPWLDGKFAKNGFLFERKWAGLV 420
           FYGKLLARAARLALIAEEV++A  V+PAVVKFLK G+QPWLDGKF +NGFL++RKWAGLV
Sbjct: 361 FYGKLLARAARLALIAEEVDYAAGVIPAVVKFLKTGVQPWLDGKFGRNGFLYDRKWAGLV 420

Query: 421 TKNGATSQTEDFGFGIYNDHHFHLGYYVYSIAVLAKLDPNWGNQYKSKAYALVNDYMNFI 480
           TKNGATS+TEDFGFGIYNDHHFHLGY+VYSIAVLAKLD NWGNQYK+ AYALV DYMN+ 
Sbjct: 421 TKNGATSKTEDFGFGIYNDHHFHLGYFVYSIAVLAKLDRNWGNQYKAPAYALVYDYMNYR 480

Query: 481 PKTSQFSIPFRNFDFWKLHSWAAGLTEFPDGRNQESTSEAVNAYYAAALMGMAYGDKALT 540
            K SQFSIPFRNFDFWKLHSWAAGLTEFPDGRNQESTSEA+NAYYAAALMG+AYGD++LT
Sbjct: 481 SKNSQFSIPFRNFDFWKLHSWAAGLTEFPDGRNQESTSEAINAYYAAALMGLAYGDESLT 540

Query: 541 DAAAALTAAEIAAAQTWWHVKKEG--SIYDQGFAGENRVVGILWSAARESRLWFAPPEWR 600
              +ALTAAEIAAAQTWWHV + G  SIYD+GF  ENRVVGILWS ARESRLWFA  EWR
Sbjct: 541 AVGSALTAAEIAAAQTWWHVNRGGRSSIYDEGFTEENRVVGILWSGARESRLWFATAEWR 600

Query: 601 ECRVGIQVLPVLPVTEKVFSDEEFVRELVEWVAPALEREDAGEGWKGFAYALEGIYDWKS 660
           ECRVGIQVLPVLPVTE+VF+DE FV+E+VEWV+PALEREDAGEGWKGF YALEGIYD ++
Sbjct: 601 ECRVGIQVLPVLPVTERVFADEGFVKEVVEWVSPALEREDAGEGWKGFVYALEGIYDKEN 660

Query: 661 GVEKIKKLKKHDDGNSLSNLLWWIYSRPAVKG 686
            V K+KKLKKHDDGNSLSNLLWWIYSRP  +G
Sbjct: 661 AVGKVKKLKKHDDGNSLSNLLWWIYSRPERQG 686

BLAST of Sed0027875 vs. NCBI nr
Match: KAG6592332.1 (hypothetical protein SDJN03_14678, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1132.9 bits (2929), Expect = 0.0e+00
Identity = 564/693 (81.39%), Postives = 613/693 (88.46%), Query Frame = 0

Query: 1   METPKPWLICVFFLQLLALFP---PPSAAQFPFPETTSTAVPDPTKFFSPSLLSSPLPTN 60
           ME P PWL+  F L L A F    PPS+AQFPFPETTSTAVPDPTKFFSPSLLSSPLPTN
Sbjct: 1   MEAPNPWLLPFFLLLLAAAFSDQLPPSSAQFPFPETTSTAVPDPTKFFSPSLLSSPLPTN 60

Query: 61  SFFQNFVLNNGDSPEYIHPYLMRIANSSLSVSYPSRTSNSSITQLPVFPDLTISALNRAS 120
           SFFQNFVLNNGD PEYIHPYL+R ANSSLSVSYPSRTSNSSITQLP  PDLTIS+     
Sbjct: 61  SFFQNFVLNNGDLPEYIHPYLIRTANSSLSVSYPSRTSNSSITQLPFSPDLTISS----- 120

Query: 121 PVSNRTHFVSSFSDLGVDLDIGDFRFHLVRGSPYLTFSVLKTSSVSIST-GYGVRSVDSS 180
            VSN+THFVSSFSDL VDLDIGDFRFHLVRGSPYLTFSVLK SSVSIST G GV SVDS 
Sbjct: 121 -VSNKTHFVSSFSDLAVDLDIGDFRFHLVRGSPYLTFSVLKNSSVSISTGGNGVGSVDSY 180

Query: 181 GDSTKHVVRLSNGRNWVVYSTSAIGLIKSKNNEII-SGGFSGVIRVAVLPDSKSESEKIL 240
            DSTKH++RLSNGR WVVYST+AI L+K K++EI+ SGGFSGVIRVAVLP+S +E+E+IL
Sbjct: 181 DDSTKHIIRLSNGRTWVVYSTAAIYLMKGKSSEIVTSGGFSGVIRVAVLPNSAAETEQIL 240

Query: 241 DLYRGCYPVSGFAKLSENYGFQYKWQKKGSGDLLMLAHPLHRRILPGNQSVLQNLRYGSI 300
           D Y GCYPVSG+AKLS N+GF YKWQKKGSG LLMLAHPLHRR+L  N +VLQN +YGSI
Sbjct: 241 DRYSGCYPVSGYAKLSGNFGFSYKWQKKGSGGLLMLAHPLHRRVLSENLTVLQNFKYGSI 300

Query: 301 DGDLVGVVGDSWDLNFNPIPVTWHSINGINNKFFPEIVAALKKDVSILNVTDLSSTPASY 360
           DGDLVGVVGDSWDLNF PIP+TWHSINGI  KFFPEIVAALK DV  LNVT+LSST ASY
Sbjct: 301 DGDLVGVVGDSWDLNFAPIPITWHSINGIERKFFPEIVAALKHDVGTLNVTELSSTAASY 360

Query: 361 FYGKLLARAARLALIAEEVNFAGDVVPAVVKFLKNGIQPWLDGKFAKNGFLFERKWAGLV 420
           FYGKLLARAARLALIAEEV++A  V+PAVVKFLK G+QPWLDGKF +NGFL++RKWAGLV
Sbjct: 361 FYGKLLARAARLALIAEEVDYAAGVIPAVVKFLKTGVQPWLDGKFGRNGFLYDRKWAGLV 420

Query: 421 TKNGATSQTEDFGFGIYNDHHFHLGYYVYSIAVLAKLDPNWGNQYKSKAYALVNDYMNFI 480
           TKNGATS+TEDFGFGIYNDHHFHLGY+VYSIAVLAKLD NWGNQYK+ AYALV DYMN+ 
Sbjct: 421 TKNGATSKTEDFGFGIYNDHHFHLGYFVYSIAVLAKLDRNWGNQYKAPAYALVYDYMNYR 480

Query: 481 PKTSQFSIPFRNFDFWKLHSWAAGLTEFPDGRNQESTSEAVNAYYAAALMGMAYGDKALT 540
            K SQFSIPFRNFDFWKLHSWAAGLTEFPDGRNQESTSEA+NAYYAAALMG+AYGD++LT
Sbjct: 481 SKNSQFSIPFRNFDFWKLHSWAAGLTEFPDGRNQESTSEAINAYYAAALMGLAYGDESLT 540

Query: 541 DAAAALTAAEIAAAQTWWHVKKEG--SIYDQGFAGENRVVGILWSAARESRLWFAPPEWR 600
              +ALTAAEIAAAQTWWHV + G  SIYD+GF  ENRVVGILWS ARESRLWFA  EWR
Sbjct: 541 AVGSALTAAEIAAAQTWWHVNRGGRSSIYDEGFTEENRVVGILWSGARESRLWFATAEWR 600

Query: 601 ECRVGIQVLPVLPVTEKVFSDEEFVRELVEWVAPALEREDAGEGWKGFAYALEGIYDWK- 660
           ECRVGIQVLPVLPVTE+VF+DE FV+E+VEWV+PALEREDAGEGWKGF YALEGIYD K 
Sbjct: 601 ECRVGIQVLPVLPVTERVFADEGFVKEVVEWVSPALEREDAGEGWKGFVYALEGIYDNKE 660

Query: 661 SGVEKIKKLKKHDDGNSLSNLLWWIYSRPAVKG 686
           + V K+KKLKKHDDGNSLSNLLWWIYSRP  +G
Sbjct: 661 NAVGKVKKLKKHDDGNSLSNLLWWIYSRPERQG 687

BLAST of Sed0027875 vs. NCBI nr
Match: XP_022925518.1 (probable endo-1,3(4)-beta-glucanase ARB_01444 [Cucurbita moschata])

HSP 1 Score: 1128.6 bits (2918), Expect = 0.0e+00
Identity = 562/694 (80.98%), Postives = 614/694 (88.47%), Query Frame = 0

Query: 1   METPKPWLICVFFLQLLALF----PPPSAAQFPFPETTSTAVPDPTKFFSPSLLSSPLPT 60
           ME P PWL+  F L LLA       PPS+AQFPFPETTSTAVPDPTKFFSPSLLSSPLPT
Sbjct: 1   MEAPNPWLLPFFLLLLLAAAFSDQLPPSSAQFPFPETTSTAVPDPTKFFSPSLLSSPLPT 60

Query: 61  NSFFQNFVLNNGDSPEYIHPYLMRIANSSLSVSYPSRTSNSSITQLPVFPDLTISALNRA 120
           NSFFQNFVLNNGD PEYIHPYL+R ANSSLSVSYPSRTSNSSITQLP  PDLTIS+    
Sbjct: 61  NSFFQNFVLNNGDLPEYIHPYLIRTANSSLSVSYPSRTSNSSITQLPFSPDLTISS---- 120

Query: 121 SPVSNRTHFVSSFSDLGVDLDIGDFRFHLVRGSPYLTFSVLKTSSVSIST-GYGVRSVDS 180
             VSN+THFVSSFSDL VDLDIGDFRFHLVRGSPYLTFSVLKTSS+SIST G GV SVDS
Sbjct: 121 --VSNKTHFVSSFSDLAVDLDIGDFRFHLVRGSPYLTFSVLKTSSISISTGGNGVGSVDS 180

Query: 181 SGDSTKHVVRLSNGRNWVVYSTSAIGLIKSKNNEII-SGGFSGVIRVAVLPDSKSESEKI 240
              STKH++RLSNGR+WVVYST+AI L+K K++EI+ SGGFSGVIRVAVLP+S +E+E+I
Sbjct: 181 YDGSTKHIIRLSNGRSWVVYSTAAIYLMKGKSSEIVTSGGFSGVIRVAVLPNSAAETEQI 240

Query: 241 LDLYRGCYPVSGFAKLSENYGFQYKWQKKGSGDLLMLAHPLHRRILPGNQSVLQNLRYGS 300
           LD Y GCYPVSG+AKLS N+GF YKWQKKGSG LLMLAHPLHRR+L  N +VLQN +YGS
Sbjct: 241 LDRYSGCYPVSGYAKLSGNFGFSYKWQKKGSGGLLMLAHPLHRRVLSENLTVLQNFKYGS 300

Query: 301 IDGDLVGVVGDSWDLNFNPIPVTWHSINGINNKFFPEIVAALKKDVSILNVTDLSSTPAS 360
           IDGDLVGVVGDSWDLNF PIP+TWHSINGI  KFFPEIVAALK DV  LNVT+LSST AS
Sbjct: 301 IDGDLVGVVGDSWDLNFAPIPITWHSINGIERKFFPEIVAALKHDVGTLNVTELSSTAAS 360

Query: 361 YFYGKLLARAARLALIAEEVNFAGDVVPAVVKFLKNGIQPWLDGKFAKNGFLFERKWAGL 420
           YFYGKLLARAARLALIAEEV++A  V+PAVVKFLK G+QPWLDGKF +NGFL++RKWAGL
Sbjct: 361 YFYGKLLARAARLALIAEEVDYAAGVIPAVVKFLKTGVQPWLDGKFGRNGFLYDRKWAGL 420

Query: 421 VTKNGATSQTEDFGFGIYNDHHFHLGYYVYSIAVLAKLDPNWGNQYKSKAYALVNDYMNF 480
           VTKNGATS+TEDFGFGIYNDHHFHLGY+VYSIAVLAKLD NWGNQYK+ AYALV DYMN+
Sbjct: 421 VTKNGATSKTEDFGFGIYNDHHFHLGYFVYSIAVLAKLDRNWGNQYKAPAYALVYDYMNY 480

Query: 481 IPKTSQFSIPFRNFDFWKLHSWAAGLTEFPDGRNQESTSEAVNAYYAAALMGMAYGDKAL 540
             K SQFSIPFRNFDFWKLHSWAAGLTEFPDGRNQESTSEA+NAYYAAALMG+AYGD++L
Sbjct: 481 RSKNSQFSIPFRNFDFWKLHSWAAGLTEFPDGRNQESTSEAINAYYAAALMGLAYGDESL 540

Query: 541 TDAAAALTAAEIAAAQTWWHVKKEG--SIYDQGFAGENRVVGILWSAARESRLWFAPPEW 600
           T   +ALTAAEIAAAQTWWHV + G  SIYD+GF  ENRVVGILWS ARESRLWFA  EW
Sbjct: 541 TAVGSALTAAEIAAAQTWWHVNRGGRSSIYDEGFTEENRVVGILWSGARESRLWFATAEW 600

Query: 601 RECRVGIQVLPVLPVTEKVFSDEEFVRELVEWVAPALEREDAGEGWKGFAYALEGIYDWK 660
           RECRVGIQVLPV+PVTE+VF+DE FV+E+VEWV+PALEREDAGEGWKGF YALEGIYD K
Sbjct: 601 RECRVGIQVLPVVPVTERVFADEGFVKEVVEWVSPALEREDAGEGWKGFVYALEGIYDNK 660

Query: 661 -SGVEKIKKLKKHDDGNSLSNLLWWIYSRPAVKG 686
            + V K+KKLKKHDDGNSLSNLLWWIYSRP  +G
Sbjct: 661 ENAVGKVKKLKKHDDGNSLSNLLWWIYSRPERQG 688

BLAST of Sed0027875 vs. NCBI nr
Match: XP_023534896.1 (probable endo-1,3(4)-beta-glucanase ARB_01444 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1128.2 bits (2917), Expect = 0.0e+00
Identity = 558/692 (80.64%), Postives = 613/692 (88.58%), Query Frame = 0

Query: 1   METPKPWLICVFFLQLLALFP---PPSAAQFPFPETTSTAVPDPTKFFSPSLLSSPLPTN 60
           ME P PWL+  F L L A F    PPS+AQFPFPETTSTAVPDP+KFFSPSLLSSPLPTN
Sbjct: 1   MEAPNPWLLPFFLLLLAAAFSDQLPPSSAQFPFPETTSTAVPDPSKFFSPSLLSSPLPTN 60

Query: 61  SFFQNFVLNNGDSPEYIHPYLMRIANSSLSVSYPSRTSNSSITQLPVFPDLTISALNRAS 120
           SFFQNFVLNNGD PEYIHPYL+R ANSSLSVSYPSRTSNSSITQLP  PDLTIS+     
Sbjct: 61  SFFQNFVLNNGDLPEYIHPYLIRTANSSLSVSYPSRTSNSSITQLPFSPDLTISS----- 120

Query: 121 PVSNRTHFVSSFSDLGVDLDIGDFRFHLVRGSPYLTFSVLKTSSVSIST-GYGVRSVDSS 180
            VSN+THFVSSFSDL VDLDIGDFRFHLVRGSPYLTFSVLKTSSVSIST G GV SVDS 
Sbjct: 121 -VSNKTHFVSSFSDLAVDLDIGDFRFHLVRGSPYLTFSVLKTSSVSISTGGNGVGSVDSY 180

Query: 181 GDSTKHVVRLSNGRNWVVYSTSAIGLIKSKNNEII-SGGFSGVIRVAVLPDSKSESEKIL 240
            DSTKH++RLSNGR WVVYST+AI L+K K++EI+ SGGF GVIRVAVLP+S +E+E+IL
Sbjct: 181 DDSTKHIIRLSNGRTWVVYSTAAIYLMKGKSSEIVTSGGFFGVIRVAVLPNSAAETEQIL 240

Query: 241 DLYRGCYPVSGFAKLSENYGFQYKWQKKGSGDLLMLAHPLHRRILPGNQSVLQNLRYGSI 300
           D Y GCYPVSG+AKLS ++GF YKWQKKGSG LLMLAHPLHRR+L  N +VLQN +YGSI
Sbjct: 241 DRYSGCYPVSGYAKLSGSFGFSYKWQKKGSGGLLMLAHPLHRRVLSENLTVLQNFKYGSI 300

Query: 301 DGDLVGVVGDSWDLNFNPIPVTWHSINGINNKFFPEIVAALKKDVSILNVTDLSSTPASY 360
           DGDLVGVVGDSWDLNF PIP+TWHSINGI  KFFPEIVAALK DV  LNVT+LSST ASY
Sbjct: 301 DGDLVGVVGDSWDLNFAPIPITWHSINGIERKFFPEIVAALKHDVGTLNVTELSSTAASY 360

Query: 361 FYGKLLARAARLALIAEEVNFAGDVVPAVVKFLKNGIQPWLDGKFAKNGFLFERKWAGLV 420
           FYGKLLARAARLALIAEEV++A  V+PAVVKFLK G+QPWLDGKF +NGFL++RKWAGLV
Sbjct: 361 FYGKLLARAARLALIAEEVDYAAGVIPAVVKFLKTGVQPWLDGKFGRNGFLYDRKWAGLV 420

Query: 421 TKNGATSQTEDFGFGIYNDHHFHLGYYVYSIAVLAKLDPNWGNQYKSKAYALVNDYMNFI 480
           TKNGATS+TEDFGFGIYNDHHFHLGY+VYSIAVLAKLD NWG+QYK+ AYALV DYMN+ 
Sbjct: 421 TKNGATSKTEDFGFGIYNDHHFHLGYFVYSIAVLAKLDRNWGSQYKAPAYALVYDYMNYR 480

Query: 481 PKTSQFSIPFRNFDFWKLHSWAAGLTEFPDGRNQESTSEAVNAYYAAALMGMAYGDKALT 540
            K +QFSIPFRNFDFWKLHSWAAGLTEFPDGRNQESTSEA+NAYYAAALMG+AYGD++LT
Sbjct: 481 SKNTQFSIPFRNFDFWKLHSWAAGLTEFPDGRNQESTSEAINAYYAAALMGLAYGDESLT 540

Query: 541 DAAAALTAAEIAAAQTWWHVKKEG--SIYDQGFAGENRVVGILWSAARESRLWFAPPEWR 600
              +ALTAAEIAAAQTWWHV + G  SIYD+GF  ENRVVGILWS ARESRLWFA  EWR
Sbjct: 541 AVGSALTAAEIAAAQTWWHVNRGGRSSIYDEGFTEENRVVGILWSGARESRLWFATAEWR 600

Query: 601 ECRVGIQVLPVLPVTEKVFSDEEFVRELVEWVAPALEREDAGEGWKGFAYALEGIYDWKS 660
           ECRVGIQVLPV+PVTE+VF+DE FV+E+VEWV+PALEREDAGEGWKGF YALEGIYD ++
Sbjct: 601 ECRVGIQVLPVVPVTERVFADEGFVKEVVEWVSPALEREDAGEGWKGFVYALEGIYDKEN 660

Query: 661 GVEKIKKLKKHDDGNSLSNLLWWIYSRPAVKG 686
            V K+KKLKKHDDGNSLSNLLWWIYSRP  +G
Sbjct: 661 AVGKVKKLKKHDDGNSLSNLLWWIYSRPERQG 686

BLAST of Sed0027875 vs. ExPASy Swiss-Prot
Match: D4AZ24 (Probable endo-1,3(4)-beta-glucanase ARB_01444 OS=Arthroderma benhamiae (strain ATCC MYA-4681 / CBS 112371) OX=663331 GN=ARB_01444 PE=1 SV=1)

HSP 1 Score: 188.3 bits (477), Expect = 2.8e-46
Identity = 199/720 (27.64%), Postives = 308/720 (42.78%), Query Frame = 0

Query: 26  AQFPFPETTSTAVPDPTKFFSPSLLSSPLPTNSFFQNFVLNNGDSPEYIHPYLM------ 85
           A+ P P    +    P K       + P+ TN F+ NF L N  S  + HPY M      
Sbjct: 198 AKDPIPANIKSRDDHPVKANHIENPTGPISTNKFYANFFLGNQTSTTFTHPYTMIWAKGD 257

Query: 86  -RIANSSLSVSY--PS-RTSNSSITQLPVFP-----------DLTISALNRASPVSNRTH 145
              ++  +++S+  PS R +     +LP  P            L +SA       +    
Sbjct: 258 KNASSFGMAISHVEPSQRATGEPNNKLPGNPVRYYINPVGIKSLVLSASELKESTTMSVA 317

Query: 146 FVSSFSDLGVDLDIG----DFRFHLVRGSPYLTFSVLKTSSVSISTGYGVRSVDSSGDST 205
              +FS   +    G       F LV+G  ++T ++      +I +    R V+ +G   
Sbjct: 318 KPQAFSAQAILRPTGGSSESITFPLVQGMGFIT-AIYNNLQPAIQSAVLFRKVEPAGSPQ 377

Query: 206 ----KHVVRLSNGRNWVVY---STSAIGLIKSKNNEIISG--GFSGVIRVAVLPDSKSES 265
               K+ + L + +NW++Y      A   +K + N++ISG  GF GVI+VA  P S  E 
Sbjct: 378 GGIFKYKITLEDDKNWLLYVIPENGADPKLKLEGNKLISGPTGFKGVIQVAKNP-SAEEG 437

Query: 266 EKILDLYRGCY----PVSGFAKLSENYGFQYKWQKKGSG-DLLMLAHPLH-RRILPGNQS 325
           E I D   G Y     +SG         +++ ++K G G  L+M A P H        ++
Sbjct: 438 EGIYDKSAGSYATDIKISGSVGTDGTGTYKFSFEKAGKGAPLVMYALPHHVESFDDATKN 497

Query: 326 VLQNLRYG-SIDGDLVGVVGDSWDLNFNPIPVT-----WHS--------INGINNKFFPE 385
             +N++   +  G     VGDSW +    +P++     W            G  N     
Sbjct: 498 TKKNMKLSTTTKGMATACVGDSWTMVEGNLPLSMDFAPWKPGSSSQVTLSEGAKNAIKAV 557

Query: 386 IVAALKKDVSILNVTDLSSTPASYFYGKLLARAARLALIAEEVNFAGDVVPAVVKFLKNG 445
               L +D+ +   T+L+S    YF GK L + A      +E+          +  LK  
Sbjct: 558 AGNELSQDMEL--QTNLNS---MYFSGKGLNKFAGAIYTVQELVGDKAAASGPLNSLKES 617

Query: 446 IQPWLDGKFAKNGFLFERKWAGLVT----KNGATSQTEDFGFGIYNDHHFHLGYYVYSIA 505
            + ++D K  +   +++  W G+V+    + G T    DFG  +YNDHHFH GY++ + A
Sbjct: 618 FKRFVDNK-QQIPLVYDNVWKGVVSSGTYEKGDTGL--DFGNTLYNDHHFHYGYFILTAA 677

Query: 506 VLAKLDPNWGNQYKSKAYALVNDYMNFIPKTSQFSIPF-RNFDFWKLHSWAAGLTEFPDG 565
           +L KLDP W +  K+    LV D  N +     F  PF R FD++  HSWA GL E  DG
Sbjct: 678 ILGKLDPAWLDANKAYVNMLVRDSGNSVDNDEHF--PFSRAFDWYHGHSWAKGLFESSDG 737

Query: 566 RNQESTSEAVNAYYAAALMGMAYGDKALTDAAAALTAAEIAAAQTWWHVKKEGSIYDQGF 625
           ++QESTSE     YA  + G   GDK++      +          ++ +K +     + F
Sbjct: 738 KDQESTSEDTMYAYAIKMWGKTSGDKSMEARGNLMLGILARTLNNYFLMKNDNVNQPKNF 797

Query: 626 AGENRVVGILWSAARESRLWFAPPEWRECRVGIQVLPVLPVTEKVFSDEEFVRELVEWVA 678
            G N+V GIL+    +   +F      E   GI +LP+LP +    S  EFV+E  EW A
Sbjct: 798 IG-NKVTGILFENKIDHTTYFGAN--LEYIQGIHMLPLLPNSAYTRS-AEFVKE--EWEA 857

BLAST of Sed0027875 vs. ExPASy Swiss-Prot
Match: Q09850 (Ascus wall endo-1,3(4)-beta-glucanase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=eng2 PE=1 SV=1)

HSP 1 Score: 164.5 bits (415), Expect = 4.3e-39
Identity = 177/702 (25.21%), Postives = 306/702 (43.59%), Query Frame = 0

Query: 16  LLALFPPPSAAQFPFPETTSTAVPDPTKFFSPSLLSSPLPTNSFFQNFVLNNGDSPEYIH 75
           L+ ++  P    FP     S A P P    + +L   P+ TN F+ N  +    +P + H
Sbjct: 4   LVPIYTGPINPVFP-----SRAHPSPPLGLTSNLF--PIQTNKFYGNLYIGTRHNPSWSH 63

Query: 76  PYLMRIANSS----LSVSY---PSRTSNSSITQLPV--------FPDLTISALNRAS--- 135
           PY +   N S    L++S+     R        +P            + ISA   AS   
Sbjct: 64  PYSVTWLNGSSYYGLAISHIDDSQRVFGPDPESVPCQYYFNPAGLYSIIISAREFASGNL 123

Query: 136 -PVSNRTHFVSSFSDLGVDLDIGDFRFHLVRG-----------SPYLTFSVLKTS--SVS 195
             +    HF    +        G     +V G           +P    S+L +S   ++
Sbjct: 124 LSLDQSRHFSIQATLSATTSGSGTIILPIVAGMGFVSGYYTNLTPVFNSSILFSSITKIN 183

Query: 196 ISTGYGVRSVDSSGDSTKHVVRLSNGRNWVVY-----STSAIGLIKSKNNEI-ISGGFSG 255
            S GY            K+ ++L++G+ W +Y     S+S   L  + N+++  S  F+G
Sbjct: 184 FSRGY------------KYRIQLTDGKIWFLYAFPTSSSSTFNLTLASNSQLTTSTKFTG 243

Query: 256 VIRVAVLPD---SKSESEKILDLYRGCY--PVSGFAKLSENYG-FQYKWQKKGSGDL--L 315
           +I++  +P+   + S  + I D   G Y   +S  A++S   G + +++   G  +L  L
Sbjct: 244 LIQICKVPNESVNNSYPDTIYDASAGVYTTSISLSAQVSGTTGEYWFRFATAGYTNLNPL 303

Query: 316 MLAHPLHRRILPGN-QSVLQNLRYGSID-GDLVGVVGDSWDL-------NFNPIPVTWHS 375
           M A P H +    + Q+    L   S   G +      +W L           +P+ W  
Sbjct: 304 MFALPHHMQSFGSDTQAYKTGLGLASTTMGIMFAYATKTWHLIEKNLPTQVGFLPIPW-- 363

Query: 376 INGINNKFFPEIVAALK----KDVSILNVTDLSSTPASYFYGKLLARAARLALIAEEVNF 435
            NG +N + P  +AA++     D++  +V + S+  + Y  GK++A  A++ L+A  +  
Sbjct: 364 -NGGSNTYSPTALAAIRAACATDIN-FDVVNASNLDSMYTSGKIVAMYAQVCLVASRILG 423

Query: 436 AGDVVPAVVKFLKNGIQPWLDGKFAKN----GFLFERKWAGLVTKNGATSQTEDFGFGIY 495
              +    +  LK  +      +F  N      +++  + G+++  G +S   D+G   Y
Sbjct: 424 DSTLTNTGLTKLKQAM-----ARFTTNTQMYPLVYDTTYKGIISTAGYSSPLADYGNTYY 483

Query: 496 NDHHFHLGYYVYSIAVLAKLDPNW-GNQYKSKAYALVNDYMNFIPKTSQFSIPFRNFDFW 555
           NDHHFH GY++Y+ AV+  LDP+W  N       AL+ D  N     + F++ FRNFD++
Sbjct: 484 NDHHFHWGYHIYACAVIGLLDPSWLVNDNIRYVNALLRDSANPSESDTYFAM-FRNFDWF 543

Query: 556 KLHSWAAGLTEFPDGRNQESTSEAVNAYYAAALMGMAYGDKALTDAAAALTAAEIAAAQT 615
             HSWA G+ E  DG+++ESTSE  N  YA  L GM   D  L + A  + A    +  T
Sbjct: 544 VGHSWATGIFESGDGKDEESTSEDFNFLYATKLWGMVRNDTVLINRANLMLAVLKNSLNT 603

Query: 616 WWHVKKEGSIYDQGFAGENRVVGILWSAARESRLWFAPPEWRECRVGIQVLPVLPVTEKV 654
           + ++    S+      G N V GI +    +   +F+  E+  C+ GI ++P  P++  +
Sbjct: 604 YIYMTPTTSVQPSQILG-NYVTGITFMNKVDYATYFSAEEY--CKQGIHMIPTTPISGYL 663

BLAST of Sed0027875 vs. ExPASy Swiss-Prot
Match: Q12168 (Endo-1,3(4)-beta-glucanase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=ACF2 PE=1 SV=1)

HSP 1 Score: 162.2 bits (409), Expect = 2.1e-38
Identity = 163/648 (25.15%), Postives = 277/648 (42.75%), Query Frame = 0

Query: 53  PLPTNSFFQNFVLNNGDSPEYIHPYLMRIANSSLSVSYPSRTSNSSITQLPVF------P 112
           PL TN F+ N +L++   P + HPY +     S         +N ++    VF      P
Sbjct: 110 PLETNKFYTNMLLDDNTQPIWTHPYSIWF---SRDPELFGLAANHTLASQRVFDTTTNPP 169

Query: 113 DLTISALNRASPVSNRTHFVSS------FSD-------LGVDLDIGDF-RFHLVRGSPYL 172
               +  N  S V     FVSS      F D       L + L    F  F LV+G  ++
Sbjct: 170 RFYFNPTNIKSFVFKAREFVSSNDIKLEFRDMKHMSMCLLMSLSSSQFIEFPLVQGMGFV 229

Query: 173 TFSVLKTSSVSISTGYGVRSVDSSGDST---KHVVRLSNGRNWVVYSTS---------AI 232
           T ++       + +  G RS++    +    K+ ++L N RNW++Y TS          I
Sbjct: 230 T-AIYHDLGFELRSAVGFRSLERISVNERYGKYNIQLENNRNWILYLTSPDYSFPQDFQI 289

Query: 233 GLIKSKNNEIISGGFSGVIRVAVLPDSKSESEKILDLYRGCYPVSGFAKLS--------E 292
            L+ S N  I S   +G+I         ++S   +D+  GCYPV  +  LS         
Sbjct: 290 SLLDS-NTIISSHKINGLI-----CQLSADSVPSIDMAAGCYPV--YCDLSGQTVDEHFT 349

Query: 293 NYGFQYKWQ-KKGSGDLLMLAHPLH----------RRILPGNQSVLQNLRYGSIDGDLVG 352
           NY F Y       SG  LM A P H          R I     S ++ L  G +      
Sbjct: 350 NYRFNYTVAGYSQSGTTLMYALPHHKAAFTPEMQEREIASSLDSTVKGLMTGYLTNSFDM 409

Query: 353 VVGDSWDLNFNPIPVTWHSINGINNKFFPEIVAALKKDVSILNVTDLSSTPASYFYGKLL 412
            V    +L F P+ ++ +     + +   +I  A  ++V + +    S+  + YF GK+L
Sbjct: 410 QVQVPQELGFEPVALSLNKKADYSQEKLSKIREAAVQEVQLSDPQQESNIDSMYFSGKIL 469

Query: 413 ARAARLALIAEEVNFAGDVVPAVVKFLKNGIQPWLDGKFAKNGFLFERKWAGLVTKNGAT 472
           A+ A +  +   +    ++   ++  L   ++ ++  +       ++  W G+++     
Sbjct: 470 AKYAWILYVTHYILHDENLTKELLSKLTIAMERFISNQQVL-PLNYDVSWKGIISSG--- 529

Query: 473 SQTEDFGFGIYNDHHFHLGYYVYSIAVLAKLDP--------NWGNQYKSKAYALVNDYMN 532
           S ++DFG   YNDHHFH  Y+V + A+++ +D         +W    +     L+ DY  
Sbjct: 530 SSSQDFGNSYYNDHHFHYSYHVITAAIISLVDSDLSGVTNNSWLENNRDWVECLIRDYSG 589

Query: 533 FIPKTSQFSIPFRNFDFWKLHSWAAGLTEFPDGRNQESTSEAVNAYYAAALMGMAYGDKA 592
            +     +   FR+FD++  HSWA GL    DG+++ESTSE VN+ YA  L G+  G+  
Sbjct: 590 -VDNDDPYFPQFRSFDWFNGHSWAKGLFPSGDGKDEESTSEDVNSCYAIKLWGLVTGNSK 649

Query: 593 LTDAAAALTAAEIAAAQTWWHVKKEGSIYDQGFAGENRVVGILWSAARESRLWFA-PPEW 641
           LTD A           Q+++  +   ++  + F G N+V GIL+    +   +F   P++
Sbjct: 650 LTDIANLQLGIMRNVFQSYFLYESNNTVQPKEFIG-NKVSGILFENKIDHATYFGMEPQY 709

BLAST of Sed0027875 vs. ExPASy Swiss-Prot
Match: P53753 (Endo-1,3(4)-beta-glucanase 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DSE4 PE=1 SV=1)

HSP 1 Score: 154.1 bits (388), Expect = 5.8e-36
Identity = 172/683 (25.18%), Postives = 301/683 (44.07%), Query Frame = 0

Query: 31   PETTSTAVPDPTKFFSPSLLSSPLPTNSFFQNFVLNNGDSPEYIHPYLMRIANSSLSVSY 90
            P T     P+P           P+ TN F+ N ++ + +SP +++PY +    SS   SY
Sbjct: 411  PPTVFDRSPNPMSLADGVSNDGPIQTNKFYTNLIVGSQESPAFVYPYSLWKYTSS---SY 470

Query: 91   PSRTSNSSITQLPV-------FPDLTISALNRASPVSNRTHFVSSFSDLGVDLDIGDFR- 150
                 ++++ Q            +  ++ L  A  V + ++F SS +    ++ +   R 
Sbjct: 471  GFAVQHTTVDQYSYGGYDSSGNAEYLVNPLGIAHVVFSASNFDSSMTMQVDEMTLSSTRV 530

Query: 151  -------------FHLVRGSPYLTFSVLKTSSVSISTGYGVRSVDSSGDST------KHV 210
                           LV+G  + T     + +  I +  G  ++ S   S       K+ 
Sbjct: 531  VLSESNDSSNYLEIPLVQGMGFATGIYHGSLNAKIGSSVGFNTIVSESSSNLAQGILKYR 590

Query: 211  VRLSNGRNWVVY-------STSAIGLIKSKNNEI-ISGGFSG-VIRVAVLPDSKSESEKI 270
            + L NG  W+ Y       +++   L  S   EI  S    G +I++AV P S+++ E  
Sbjct: 591  ITLLNGVTWLCYVIGPDDLTSTDFSLEVSSEYEIKASASVDGLIIQLAVAP-SETDYEVF 650

Query: 271  LDLYRGCYPVSGFAKL-------SENYGFQYKWQ-KKGSGDLLMLAHPLHRR----ILPG 330
             D   G Y V+ F KL       +  Y F Y  Q +  SG  ++ A P H      I+  
Sbjct: 651  YDQAAGMY-VTNF-KLQGVSDGSTATYEFSYTTQGESASGSTMIFALPHHESSFSDIMQD 710

Query: 331  NQSVLQ--NLRYGSIDGDLVGVVGDSWDLNFNPIPVTWHSINGIN----NKFFPEIVAAL 390
              + +Q  +   G ++G L   +  S  LN     + W S  G N    +K   +++A +
Sbjct: 711  YYTGIQLASTTKGVMNGYLTTSLQFSTSLNRQISWLPWSSQLGSNLLEYSKEQLQLLAEV 770

Query: 391  KKDVSILNVTDLSSTPASYFYGKLLARAARLALIAEEVNFAGDVVPAVVKFLKNGIQPWL 450
                  +++++  S   +Y+ GK++ + + + L   E+        + ++ +K+     L
Sbjct: 771  ANSELQVSISESISGLNTYYLGKVIDKYSYILLTVSEIIQDEASTKSTLENIKSAFDILL 830

Query: 451  DGKFAKNGFLFERKWAGLVTKN--GATSQTEDFGFGIYNDHHFHLGYYVYSIAVL----A 510
              +      +++ K+ GLV+    G+TS   DFG   YNDHHFH GY +++ AV+    +
Sbjct: 831  QNE-QTYPLIYDTKFNGLVSSGDWGSTSTQYDFGNTYYNDHHFHYGYIIHAAAVIGYVDS 890

Query: 511  KLDPNWGNQYKSKAYALVNDYMNFIPKTSQFSIPFRNFDFWKLHSWAAGLTEFPDGRNQE 570
            KL+  W    K    +LV D  N   K   F+   R FD++  HSWAAGL E  +G+N+E
Sbjct: 891  KLNGTWAADNKDWVNSLVRDVANPSEKDEYFA-QSRMFDWFNGHSWAAGLYENGNGKNEE 950

Query: 571  STSEAVNAYYAAALMGMAYGDKALTDAAAALTAAEIAAAQTWWHVKKEGSIYDQGFAGEN 630
            S+SE  N  YA  L G   GD+++      + +    A   +++ + + ++  +   G N
Sbjct: 951  SSSEDYNFAYAMKLWGATIGDQSMELRGDLMISIMKDAMNDYFYYQNDNTVEPEEIIG-N 1010

Query: 631  RVVGILWSAARESRLWFAPPEWRECRVGIQVLPVLPVTEKVFSDEEFVRELVEW---VAP 651
            +V GIL+    +   +F      E   GI +LP+ PV+  + S E FV E  EW   + P
Sbjct: 1011 KVSGILFDNIIDYTTYFGTN--TEYIHGIHMLPITPVSSNIRS-ETFVEE--EWQTKIEP 1070

BLAST of Sed0027875 vs. ExPASy Swiss-Prot
Match: Q9UT45 (Primary septum endo-1,3(4)-beta-glucanase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=eng1 PE=3 SV=1)

HSP 1 Score: 136.3 bits (342), Expect = 1.3e-30
Identity = 123/488 (25.20%), Postives = 217/488 (44.47%), Query Frame = 0

Query: 181 KHVVRLSNGRNWVVY-STSAIGLIKSKNNEII-SGGFSGVIRVAVLPDSKSESEKILDLY 240
           K+ V +S+   W++Y    ++ L +S +  ++ S  F+G I++A +P     +E + D Y
Sbjct: 226 KYRVTMSDNHVWLIYIYGDSLTLTESTSQMLVGSNTFNGYIQIAKIPLGDGTAEALYDTY 285

Query: 241 RGCY----PVSGFAKLSENYGFQYKWQKKGSGD------LLMLAHPLHRRILPGNQSVLQ 300
            G Y     +SG+ + +  Y   Y +    +GD        +L H +   +      V  
Sbjct: 286 AGVYITGISISGYVEDAVGY---YSFDFTTAGDTSVEPLFFLLPHQVDTAV--SGTKVTS 345

Query: 301 NLRYGSIDGDLVGVVGDS--------WDLNFNPIPVTWHSINGINNKFFPEIVAALKKDV 360
            +    + GD+    G+S         D+ F P   T   I G + +   EI+A +    
Sbjct: 346 IVLASLVSGDMNAAAGNSITFAEAIPQDIGFLPWSPTGGQI-GYSEEAL-EIIAEVAGTE 405

Query: 361 SILNVTDLSSTPASYFYGKLLARAARLALIAEEVNFAGDVVPAVVKFLKNGIQPWLDGKF 420
              + +  S+  + Y+ GK+LA+ A L +   ++          ++ L+     ++D + 
Sbjct: 406 LGEDFSANSNLNSMYYSGKVLAKYAMLCVTINDILGDETSSEQCIQKLEAAFARFVDNQ- 465

Query: 421 AKNGFLFERKWAGLVTKNGATSQT-EDFGFGIYNDHHFHLGYYVYSIAVLAKLDPNWGNQ 480
                 ++  W G+V+  G +  +  DFG   YNDHHFH GY+V++ AV+  +DP+W N 
Sbjct: 466 QIYPLTYDNTWKGVVSVAGLSGDSLADFGNSYYNDHHFHYGYFVFTAAVIGHIDPDWINT 525

Query: 481 YKSKAYA--LVNDYMNFIPKTSQFSIP-FRNFDFWKLHSWAAGLTEFPDGRNQESTSEAV 540
             +K +   LV D  N  P ++    P  R  D +  H WA+GL E  DG+++ESTSE  
Sbjct: 526 GNNKEWVNFLVRDVAN--PSSNDPYFPKHRMIDIYHGHGWASGLFESNDGKDEESTSEDY 585

Query: 541 NAYYAAALMGMAYGDKALTDAAAALTAAEIAAAQTWWHVKKEGSIYDQGFAGENRVVGIL 600
           N ++   L G   GD  + D A  +   E  A   +  +  +G++        N V GI 
Sbjct: 586 NFFFGMKLWGQVIGDSDMEDRANIILGIERNALNKYM-LYADGNVQPTSMQ-PNYVAGIT 645

Query: 601 WSAARESRLWFAPPEWRECRVGIQVLPVLPVTEKVFSDEEFVRELVEWVAPALEREDAGE 645
           +        +F      E   GI +LP+ P++  +      + E    +A  ++  D+  
Sbjct: 646 FMNKITHTTYFGTN--IEYIQGIHMLPITPISAFIRGPSFVLAEWNALLASVIDYVDS-- 697

BLAST of Sed0027875 vs. ExPASy TrEMBL
Match: A0A6J1EFF1 (Endo-1,3(4)-beta-glucanase OS=Cucurbita moschata OX=3662 GN=LOC111432794 PE=3 SV=1)

HSP 1 Score: 1128.6 bits (2918), Expect = 0.0e+00
Identity = 562/694 (80.98%), Postives = 614/694 (88.47%), Query Frame = 0

Query: 1   METPKPWLICVFFLQLLALF----PPPSAAQFPFPETTSTAVPDPTKFFSPSLLSSPLPT 60
           ME P PWL+  F L LLA       PPS+AQFPFPETTSTAVPDPTKFFSPSLLSSPLPT
Sbjct: 1   MEAPNPWLLPFFLLLLLAAAFSDQLPPSSAQFPFPETTSTAVPDPTKFFSPSLLSSPLPT 60

Query: 61  NSFFQNFVLNNGDSPEYIHPYLMRIANSSLSVSYPSRTSNSSITQLPVFPDLTISALNRA 120
           NSFFQNFVLNNGD PEYIHPYL+R ANSSLSVSYPSRTSNSSITQLP  PDLTIS+    
Sbjct: 61  NSFFQNFVLNNGDLPEYIHPYLIRTANSSLSVSYPSRTSNSSITQLPFSPDLTISS---- 120

Query: 121 SPVSNRTHFVSSFSDLGVDLDIGDFRFHLVRGSPYLTFSVLKTSSVSIST-GYGVRSVDS 180
             VSN+THFVSSFSDL VDLDIGDFRFHLVRGSPYLTFSVLKTSS+SIST G GV SVDS
Sbjct: 121 --VSNKTHFVSSFSDLAVDLDIGDFRFHLVRGSPYLTFSVLKTSSISISTGGNGVGSVDS 180

Query: 181 SGDSTKHVVRLSNGRNWVVYSTSAIGLIKSKNNEII-SGGFSGVIRVAVLPDSKSESEKI 240
              STKH++RLSNGR+WVVYST+AI L+K K++EI+ SGGFSGVIRVAVLP+S +E+E+I
Sbjct: 181 YDGSTKHIIRLSNGRSWVVYSTAAIYLMKGKSSEIVTSGGFSGVIRVAVLPNSAAETEQI 240

Query: 241 LDLYRGCYPVSGFAKLSENYGFQYKWQKKGSGDLLMLAHPLHRRILPGNQSVLQNLRYGS 300
           LD Y GCYPVSG+AKLS N+GF YKWQKKGSG LLMLAHPLHRR+L  N +VLQN +YGS
Sbjct: 241 LDRYSGCYPVSGYAKLSGNFGFSYKWQKKGSGGLLMLAHPLHRRVLSENLTVLQNFKYGS 300

Query: 301 IDGDLVGVVGDSWDLNFNPIPVTWHSINGINNKFFPEIVAALKKDVSILNVTDLSSTPAS 360
           IDGDLVGVVGDSWDLNF PIP+TWHSINGI  KFFPEIVAALK DV  LNVT+LSST AS
Sbjct: 301 IDGDLVGVVGDSWDLNFAPIPITWHSINGIERKFFPEIVAALKHDVGTLNVTELSSTAAS 360

Query: 361 YFYGKLLARAARLALIAEEVNFAGDVVPAVVKFLKNGIQPWLDGKFAKNGFLFERKWAGL 420
           YFYGKLLARAARLALIAEEV++A  V+PAVVKFLK G+QPWLDGKF +NGFL++RKWAGL
Sbjct: 361 YFYGKLLARAARLALIAEEVDYAAGVIPAVVKFLKTGVQPWLDGKFGRNGFLYDRKWAGL 420

Query: 421 VTKNGATSQTEDFGFGIYNDHHFHLGYYVYSIAVLAKLDPNWGNQYKSKAYALVNDYMNF 480
           VTKNGATS+TEDFGFGIYNDHHFHLGY+VYSIAVLAKLD NWGNQYK+ AYALV DYMN+
Sbjct: 421 VTKNGATSKTEDFGFGIYNDHHFHLGYFVYSIAVLAKLDRNWGNQYKAPAYALVYDYMNY 480

Query: 481 IPKTSQFSIPFRNFDFWKLHSWAAGLTEFPDGRNQESTSEAVNAYYAAALMGMAYGDKAL 540
             K SQFSIPFRNFDFWKLHSWAAGLTEFPDGRNQESTSEA+NAYYAAALMG+AYGD++L
Sbjct: 481 RSKNSQFSIPFRNFDFWKLHSWAAGLTEFPDGRNQESTSEAINAYYAAALMGLAYGDESL 540

Query: 541 TDAAAALTAAEIAAAQTWWHVKKEG--SIYDQGFAGENRVVGILWSAARESRLWFAPPEW 600
           T   +ALTAAEIAAAQTWWHV + G  SIYD+GF  ENRVVGILWS ARESRLWFA  EW
Sbjct: 541 TAVGSALTAAEIAAAQTWWHVNRGGRSSIYDEGFTEENRVVGILWSGARESRLWFATAEW 600

Query: 601 RECRVGIQVLPVLPVTEKVFSDEEFVRELVEWVAPALEREDAGEGWKGFAYALEGIYDWK 660
           RECRVGIQVLPV+PVTE+VF+DE FV+E+VEWV+PALEREDAGEGWKGF YALEGIYD K
Sbjct: 601 RECRVGIQVLPVVPVTERVFADEGFVKEVVEWVSPALEREDAGEGWKGFVYALEGIYDNK 660

Query: 661 -SGVEKIKKLKKHDDGNSLSNLLWWIYSRPAVKG 686
            + V K+KKLKKHDDGNSLSNLLWWIYSRP  +G
Sbjct: 661 ENAVGKVKKLKKHDDGNSLSNLLWWIYSRPERQG 688

BLAST of Sed0027875 vs. ExPASy TrEMBL
Match: A0A6J1I8S4 (Endo-1,3(4)-beta-glucanase OS=Cucurbita maxima OX=3661 GN=LOC111472032 PE=3 SV=1)

HSP 1 Score: 1127.9 bits (2916), Expect = 0.0e+00
Identity = 559/692 (80.78%), Postives = 615/692 (88.87%), Query Frame = 0

Query: 1   METPKPWLICVFFLQLLALFP---PPSAAQFPFPETTSTAVPDPTKFFSPSLLSSPLPTN 60
           M    PWL+  FFL L A +    PPS+AQFPFPETTSTAVPDPTKFFSPSLLSSPLPTN
Sbjct: 1   MAATNPWLL-PFFLLLAAAYSDQLPPSSAQFPFPETTSTAVPDPTKFFSPSLLSSPLPTN 60

Query: 61  SFFQNFVLNNGDSPEYIHPYLMRIANSSLSVSYPSRTSNSSITQLPVFPDLTISALNRAS 120
           SFFQNFVLN GD PEYIHPYL+R ANSSLSVSYPSRTSN+SITQLP  PDLTIS+     
Sbjct: 61  SFFQNFVLNKGDLPEYIHPYLIRTANSSLSVSYPSRTSNTSITQLPFSPDLTISS----- 120

Query: 121 PVSNRTHFVSSFSDLGVDLDIGDFRFHLVRGSPYLTFSVLKTSSVSIST-GYGVRSVDSS 180
            VSN+THFVSSFSDL VDLDIG+FRFHLVRGSPYLTFSVLKTSSVSIST G GV SVDS 
Sbjct: 121 -VSNKTHFVSSFSDLAVDLDIGEFRFHLVRGSPYLTFSVLKTSSVSISTGGNGVDSVDSY 180

Query: 181 GDSTKHVVRLSNGRNWVVYSTSAIGLIKSKNNEII-SGGFSGVIRVAVLPDSKSESEKIL 240
             STKH++RLSNGRNWVVYST+AI L+K+K++EI+ SGGFSGVIRVAVLP+S +E+E+IL
Sbjct: 181 DGSTKHIIRLSNGRNWVVYSTAAIYLMKAKSSEIVTSGGFSGVIRVAVLPNSAAETEQIL 240

Query: 241 DLYRGCYPVSGFAKLSENYGFQYKWQKKGSGDLLMLAHPLHRRILPGNQSVLQNLRYGSI 300
           D Y GCYPVSG+AKLS N+GF YKWQKKGSG LLMLAHPLHRR+LP N +VLQN +YGSI
Sbjct: 241 DRYSGCYPVSGYAKLSGNFGFSYKWQKKGSGGLLMLAHPLHRRVLPENLTVLQNFKYGSI 300

Query: 301 DGDLVGVVGDSWDLNFNPIPVTWHSINGINNKFFPEIVAALKKDVSILNVTDLSSTPASY 360
           DGDLVGVVGDSWDLNF PIP+TWHSINGI  KFFPEIVAALK DV  LNVT+LSST ASY
Sbjct: 301 DGDLVGVVGDSWDLNFAPIPITWHSINGIERKFFPEIVAALKHDVGTLNVTELSSTAASY 360

Query: 361 FYGKLLARAARLALIAEEVNFAGDVVPAVVKFLKNGIQPWLDGKFAKNGFLFERKWAGLV 420
           FYGKLLARAARLALIAEEV++A  V+PAVVKFLK GIQPWLDGKF +NGFL++RKWAGLV
Sbjct: 361 FYGKLLARAARLALIAEEVDYAAGVIPAVVKFLKTGIQPWLDGKFGRNGFLYDRKWAGLV 420

Query: 421 TKNGATSQTEDFGFGIYNDHHFHLGYYVYSIAVLAKLDPNWGNQYKSKAYALVNDYMNFI 480
           TKNGATS+TEDFGFGIYNDHHFHLGY+VYSIAVLAKLD NWGNQYK+ AYALV+DYMN+ 
Sbjct: 421 TKNGATSKTEDFGFGIYNDHHFHLGYFVYSIAVLAKLDRNWGNQYKAPAYALVHDYMNYR 480

Query: 481 PKTSQFSIPFRNFDFWKLHSWAAGLTEFPDGRNQESTSEAVNAYYAAALMGMAYGDKALT 540
            + +QFSIPFRNFDFWKLHSWAAGLTEFPDGRNQESTSEA+NAYYAAALMG+AYGD++LT
Sbjct: 481 SRNTQFSIPFRNFDFWKLHSWAAGLTEFPDGRNQESTSEAINAYYAAALMGLAYGDESLT 540

Query: 541 DAAAALTAAEIAAAQTWWHVKKEG--SIYDQGFAGENRVVGILWSAARESRLWFAPPEWR 600
              +ALTAAEIAAAQTWWHV + G  SIYD+GF  ENRVVGILWS ARESRLWFA  EWR
Sbjct: 541 AVGSALTAAEIAAAQTWWHVNRGGRSSIYDEGFTEENRVVGILWSGARESRLWFASAEWR 600

Query: 601 ECRVGIQVLPVLPVTEKVFSDEEFVRELVEWVAPALEREDAGEGWKGFAYALEGIYDWKS 660
           ECRVGIQVLPV+PVTE+VF+DE FV+E+VEWV+PALEREDAGEGWKGF YALEGIYD K+
Sbjct: 601 ECRVGIQVLPVVPVTERVFADEGFVKEVVEWVSPALEREDAGEGWKGFVYALEGIYDKKN 660

Query: 661 GVEKIKKLKKHDDGNSLSNLLWWIYSRPAVKG 686
            V K+KKLKKHDDGNSLSNLLWWIYSRP  +G
Sbjct: 661 AVGKVKKLKKHDDGNSLSNLLWWIYSRPERQG 685

BLAST of Sed0027875 vs. ExPASy TrEMBL
Match: A0A6J1DY72 (Endo-1,3(4)-beta-glucanase OS=Momordica charantia OX=3673 GN=LOC111025628 PE=3 SV=1)

HSP 1 Score: 1125.9 bits (2911), Expect = 0.0e+00
Identity = 551/690 (79.86%), Postives = 602/690 (87.25%), Query Frame = 0

Query: 1   METPKPWLICVFFLQLLALF-------PPPSAAQFPFPETTSTAVPDPTKFFSPSLLSSP 60
           M  PKPW I +FF    + F       PPPS +QFPFPET ST VPDP  FFSPSLLSSP
Sbjct: 1   MAEPKPWPISLFFFLFFSAFAAAVPDHPPPSTSQFPFPETASTVVPDPANFFSPSLLSSP 60

Query: 61  LPTNSFFQNFVLNNGDSPEYIHPYLMRIANSSLSVSYPSRTSNSSITQLPVFPDLTISAL 120
           LPTNSFFQNFVL+NGD PEYIHPYL+RIANSSLSVSYPSR+SNSSITQLP  PDLTI + 
Sbjct: 61  LPTNSFFQNFVLSNGDLPEYIHPYLIRIANSSLSVSYPSRSSNSSITQLPFVPDLTIDSF 120

Query: 121 NRASPVSNRTHFVSSFSDLGVDLDIGDFRFHLVRGSPYLTFSVLKTSSVSISTGYGVRSV 180
                  NRTHFVSSFSDL VDLDIGDFRFHLVRGSPYLTFSVL+TSSVSISTG+GVRSV
Sbjct: 121 -------NRTHFVSSFSDLAVDLDIGDFRFHLVRGSPYLTFSVLETSSVSISTGHGVRSV 180

Query: 181 DSSGDSTKHVVRLSNGRNWVVYSTSAIGLIKSKNNEII-SGGFSGVIRVAVLPDSKSESE 240
           DS   STKH+VRLSNGRNWV+YST+AI LIKS    I+ SGGFSGVIRVAVLPDS+ ESE
Sbjct: 181 DSYDFSTKHIVRLSNGRNWVLYSTAAIDLIKSSTYRIVTSGGFSGVIRVAVLPDSEGESE 240

Query: 241 KILDLYRGCYPVSGFAKLSENYGFQYKWQKKGSGDLLMLAHPLHRRILPGNQSVLQNLRY 300
           KILD YRGCYPVSGFAKLSE +G QYKWQK+GSGDLLMLAHPLHRR+LP NQ+VLQNLRY
Sbjct: 241 KILDRYRGCYPVSGFAKLSEKFGVQYKWQKRGSGDLLMLAHPLHRRLLPENQTVLQNLRY 300

Query: 301 GSIDGDLVGVV-GDSWDLNFNPIPVTWHSINGINNKFFPEIVAALKKDVSILNVTDLSST 360
           GSIDGDLVGVV G SWDLNF+PIPVTWHSINGIN +FFPEI AAL++DV  LN T+LSST
Sbjct: 301 GSIDGDLVGVVGGGSWDLNFDPIPVTWHSINGINKQFFPEIAAALRRDVKALNTTELSST 360

Query: 361 PASYFYGKLLARAARLALIAEEVNFAGDVVPAVVKFLKNGIQPWLDGKFAKNGFLFERKW 420
            +SYFYGKL+ARAARLALIAEEV+FAGDVVPAV KFL+NGIQPWLDGKF +NGF +ERKW
Sbjct: 361 ASSYFYGKLIARAARLALIAEEVDFAGDVVPAVAKFLRNGIQPWLDGKFGRNGFRYERKW 420

Query: 421 AGLVTKNGATSQTEDFGFGIYNDHHFHLGYYVYSIAVLAKLDPNWGNQYKSKAYALVNDY 480
           AGLVT+NGA+S TEDFGFG+YNDHHFHLGY+VYSIAVLAK+DPNWG +YK  AYALV+DY
Sbjct: 421 AGLVTENGASSTTEDFGFGVYNDHHFHLGYFVYSIAVLAKIDPNWGEKYKPHAYALVSDY 480

Query: 481 MNFIPKTSQFSIPFRNFDFWKLHSWAAGLTEFPDGRNQESTSEAVNAYYAAALMGMAYGD 540
           MNF PK   FSIPFRNFDFWKLHSWAAGL  FPDGRNQESTSEAVNAYYAAAL+G+AYGD
Sbjct: 481 MNFRPKKFPFSIPFRNFDFWKLHSWAAGLHPFPDGRNQESTSEAVNAYYAAALLGLAYGD 540

Query: 541 KALTDAAAALTAAEIAAAQTWWHVKKEGSIYDQGFAGENRVVGILWSAARESRLWFAPPE 600
           ++   A  AL  AEI AAQTWWHV+++  IY++GF  ENRVVGILWS ARESRLWFAP E
Sbjct: 541 ESFAAAGTALATAEILAAQTWWHVRRDSGIYERGFVEENRVVGILWSGARESRLWFAPAE 600

Query: 601 WRECRVGIQVLPVLPVTEKVFSDEEFVRELVEWVAPALEREDAGEGWKGFAYALEGIYDW 660
           WRECR+GIQVLPVLPVTE++FSDEE+VRE VEW A A+EREDAGEGWKGFAYALEGIYD 
Sbjct: 601 WRECRLGIQVLPVLPVTERLFSDEEYVREAVEWAAAAVEREDAGEGWKGFAYALEGIYDK 660

Query: 661 KSGVEKIKKLKKHDDGNSLSNLLWWIYSRP 682
           K+GVEKIKKLK HDDGNSLSNLLWWIYSRP
Sbjct: 661 KNGVEKIKKLKNHDDGNSLSNLLWWIYSRP 683

BLAST of Sed0027875 vs. ExPASy TrEMBL
Match: A0A5A7TDJ6 (Endo-1,3(4)-beta-glucanase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold169G001320 PE=3 SV=1)

HSP 1 Score: 1121.3 bits (2899), Expect = 0.0e+00
Identity = 558/688 (81.10%), Postives = 608/688 (88.37%), Query Frame = 0

Query: 2   ETPK--PWLICVFFLQLLALFPPPSAAQFPFPETTSTAVPDPTKFFSPSLLSSPLPTNSF 61
           ET K  P+ I  FFL L A    P +AQFPFPETTSTAVPDP+KFFSP+LLSSPLPTNSF
Sbjct: 3   ETSKQSPFSILFFFLLLTA---APVSAQFPFPETTSTAVPDPSKFFSPNLLSSPLPTNSF 62

Query: 62  FQNFVLNNGDSPEYIHPYLMRIANSSLSVSYPSRTSNSSITQLPVFPDLTISALNRASPV 121
           FQNFVLNNGDSPEYIHPYL+R ANSSL VSYPSR SNSSI QL  FPDL IS+LN+    
Sbjct: 63  FQNFVLNNGDSPEYIHPYLIRTANSSLFVSYPSRISNSSINQLQFFPDLVISSLNK---T 122

Query: 122 SNRTHFVSSFSDLGVDLDIGDFRFHLVRGSPYLTFSVLKTSSVSISTGYGVRSVDSSGDS 181
           SN THFVSSFSDLGVDLDIG FR+HLVRGSPYLTFSVLKTS V IST  GVRSVDS  D 
Sbjct: 123 SNATHFVSSFSDLGVDLDIGVFRYHLVRGSPYLTFSVLKTSFVLISTSNGVRSVDSYEDY 182

Query: 182 TKHVVRLSNGRNWVVYSTSAIGLIKSKNNEII-SGGFSGVIRVAVLPDSKSESEKILDLY 241
           TK+++RL+NGR+W++YS+SAI LIKSK+N+++ SGGF GVIRVAVLPDS  ESEKILD Y
Sbjct: 183 TKYIIRLNNGRSWLLYSSSAIYLIKSKSNQVVTSGGFIGVIRVAVLPDSAVESEKILDRY 242

Query: 242 RGCYPVSGFAKLSENYGFQYKWQKKGSGDLLMLAHPLHRRILPGNQSVLQNLRYGSIDGD 301
            GCYPVSGFAKLS  +GF+YKWQKKGSG LLMLAH LHR ILP +Q+VLQNLRY SIDGD
Sbjct: 243 SGCYPVSGFAKLSGGFGFEYKWQKKGSGGLLMLAHTLHREILPRDQTVLQNLRYSSIDGD 302

Query: 302 LVGVVGDSWDLNFNPIPVTWHSINGINNKFFPEIVAALKKDVSILNVTDLSSTPASYFYG 361
           L+GVVGDSWDL FNPIP+TWHSINGI++KFFPEIVAALK+DV+ LNVT+LSSTPASYFYG
Sbjct: 303 LLGVVGDSWDLKFNPIPITWHSINGIDSKFFPEIVAALKRDVATLNVTELSSTPASYFYG 362

Query: 362 KLLARAARLALIAEEVNFAGDVVPAVVKFLKNGIQPWLDGKFAKNGFLFERKWAGLVTKN 421
           KLLARAARLALIAEEVN+   V+PAVVKFLKNGIQPWL GKF KNGFL+ERKWAGLVTKN
Sbjct: 363 KLLARAARLALIAEEVNYGASVIPAVVKFLKNGIQPWLIGKFPKNGFLYERKWAGLVTKN 422

Query: 422 GATSQTEDFGFGIYNDHHFHLGYYVYSIAVLAKLDPNWGNQYKSKAYALVNDYMNFIPKT 481
           GATS  EDFGFGIYNDHHFHLGY+VYSIAVLAKLDPNWG QYK +AYAL+ DYMNF PK 
Sbjct: 423 GATSTIEDFGFGIYNDHHFHLGYFVYSIAVLAKLDPNWGKQYKPQAYALLYDYMNFRPKK 482

Query: 482 SQFSIPFRNFDFWKLHSWAAGLTEFPDGRNQESTSEAVNAYYAAALMGMAYGDKALTDAA 541
           SQFSIPFRNFDFWKLHSWAAGL EFPDGRNQESTSEAVNAYYAAALMG+AYGD +LT A 
Sbjct: 483 SQFSIPFRNFDFWKLHSWAAGLAEFPDGRNQESTSEAVNAYYAAALMGLAYGDASLTAAG 542

Query: 542 AALTAAEIAAAQTWWHVKKE-GSIYDQGFAGENRVVGILWSAARESRLWFAPPEWRECRV 601
           + LTAAEI A+QTWWHVKKE   IYD+GFA ENR+VGILWSAARESRLWFAP EWRECR+
Sbjct: 543 STLTAAEITASQTWWHVKKEKNGIYDKGFAEENRMVGILWSAARESRLWFAPAEWRECRL 602

Query: 602 GIQVLPVLPVTEKVFSDEEFVRELVEWVAPALEREDAGEGWKGFAYALEGIYDWKSGVEK 661
           GIQVLPVLPVTE++F+D  FV+E VEWV PALEREDAGEGWKGFAYALEGIYD KS VEK
Sbjct: 603 GIQVLPVLPVTERMFTDVGFVKEAVEWVQPALEREDAGEGWKGFAYALEGIYDKKSAVEK 662

Query: 662 IKKLKKHDDGNSLSNLLWWIYSRPAVKG 686
           +KKLKKHDDGNSLSNLLWWIYSRP  +G
Sbjct: 663 VKKLKKHDDGNSLSNLLWWIYSRPEGRG 684

BLAST of Sed0027875 vs. ExPASy TrEMBL
Match: A0A142BTQ9 (Endo-1,3(4)-beta-glucanase OS=Cucumis melo OX=3656 GN=glu2 PE=2 SV=1)

HSP 1 Score: 1120.5 bits (2897), Expect = 0.0e+00
Identity = 557/682 (81.67%), Postives = 604/682 (88.56%), Query Frame = 0

Query: 6   PWLICVFFLQLLALFPPPSAAQFPFPETTSTAVPDPTKFFSPSLLSSPLPTNSFFQNFVL 65
           P+ I  FFL L A    P +AQFPFPETTSTAVPDP+KFFSP+LLSSPLPTNSFFQNFVL
Sbjct: 9   PFSILFFFLLLTA---APVSAQFPFPETTSTAVPDPSKFFSPNLLSSPLPTNSFFQNFVL 68

Query: 66  NNGDSPEYIHPYLMRIANSSLSVSYPSRTSNSSITQLPVFPDLTISALNRASPVSNRTHF 125
           NNGDSPEYIHPYL+R ANSSL VSYPSR SNSSI QL  FPDL IS+LN+    SN THF
Sbjct: 69  NNGDSPEYIHPYLIRTANSSLFVSYPSRISNSSINQLQFFPDLVISSLNK---TSNATHF 128

Query: 126 VSSFSDLGVDLDIGDFRFHLVRGSPYLTFSVLKTSSVSISTGYGVRSVDSSGDSTKHVVR 185
           VSSFSDLGVDLDIG FR+HLVRGSPYLTFSVLKTS V IST  GVRSVDS  D TK+++R
Sbjct: 129 VSSFSDLGVDLDIGVFRYHLVRGSPYLTFSVLKTSFVLISTSNGVRSVDSYEDYTKYIIR 188

Query: 186 LSNGRNWVVYSTSAIGLIKSKNNEII-SGGFSGVIRVAVLPDSKSESEKILDLYRGCYPV 245
           L+NGR+WV+YS+SAI LIKSK+N+I+ SGGF GVIRVAVLPDS  ESEKILD Y GCYPV
Sbjct: 189 LNNGRSWVLYSSSAIYLIKSKSNQIVTSGGFIGVIRVAVLPDSAVESEKILDRYSGCYPV 248

Query: 246 SGFAKLSENYGFQYKWQKKGSGDLLMLAHPLHRRILPGNQSVLQNLRYGSIDGDLVGVVG 305
           SGFAKLS  +GF+YKWQKKGSG LLMLAH LHR ILP +Q+VLQNLRY SIDGDL+GVVG
Sbjct: 249 SGFAKLSGGFGFEYKWQKKGSGGLLMLAHTLHREILPRDQTVLQNLRYSSIDGDLLGVVG 308

Query: 306 DSWDLNFNPIPVTWHSINGINNKFFPEIVAALKKDVSILNVTDLSSTPASYFYGKLLARA 365
           DSWDL FNPIP+TWHSINGIN+KFFPEIVAALK+DV+ LNVT+LSSTPASYFYGKLLARA
Sbjct: 309 DSWDLKFNPIPITWHSINGINSKFFPEIVAALKRDVATLNVTELSSTPASYFYGKLLARA 368

Query: 366 ARLALIAEEVNFAGDVVPAVVKFLKNGIQPWLDGKFAKNGFLFERKWAGLVTKNGATSQT 425
           ARLALIAEEVN+   V+PAVVKFLKNGIQPWL GKF KNGFL+ERKWAGLVTKNGATS  
Sbjct: 369 ARLALIAEEVNYGASVIPAVVKFLKNGIQPWLIGKFPKNGFLYERKWAGLVTKNGATSTI 428

Query: 426 EDFGFGIYNDHHFHLGYYVYSIAVLAKLDPNWGNQYKSKAYALVNDYMNFIPKTSQFSIP 485
           EDFGFGIYNDHHFHLGY+VYSIAVLAKLDPNW  QYK +AYAL+ DYMNF PK SQFSIP
Sbjct: 429 EDFGFGIYNDHHFHLGYFVYSIAVLAKLDPNWRKQYKPQAYALLYDYMNFRPKKSQFSIP 488

Query: 486 FRNFDFWKLHSWAAGLTEFPDGRNQESTSEAVNAYYAAALMGMAYGDKALTDAAAALTAA 545
           FRNFDFWKLHSWAAGL EFPDGRNQESTSEAVNAYYAAALMG+AYGD +LT A + LTAA
Sbjct: 489 FRNFDFWKLHSWAAGLAEFPDGRNQESTSEAVNAYYAAALMGLAYGDASLTAAGSTLTAA 548

Query: 546 EIAAAQTWWHVKKE-GSIYDQGFAGENRVVGILWSAARESRLWFAPPEWRECRVGIQVLP 605
           EI A+QTWWHVKKE   IYD+GFA ENR+VGILWSAARESRLWFAP EWRECR+GIQVLP
Sbjct: 549 EITASQTWWHVKKEKNGIYDKGFAEENRMVGILWSAARESRLWFAPAEWRECRLGIQVLP 608

Query: 606 VLPVTEKVFSDEEFVRELVEWVAPALEREDAGEGWKGFAYALEGIYDWKSGVEKIKKLKK 665
           VLPVTE++F+D  FV+E VEWV PALEREDAGEGWKGFAYALEGIYD KS VEK+KKLKK
Sbjct: 609 VLPVTERMFTDVGFVKEAVEWVQPALEREDAGEGWKGFAYALEGIYDKKSAVEKVKKLKK 668

Query: 666 HDDGNSLSNLLWWIYSRPAVKG 686
           HDDGNSLSNLLWWIYSRP  +G
Sbjct: 669 HDDGNSLSNLLWWIYSRPEGRG 684

BLAST of Sed0027875 vs. TAIR 10
Match: AT5G15870.1 (glycosyl hydrolase family 81 protein )

HSP 1 Score: 739.6 bits (1908), Expect = 2.3e-213
Identity = 372/678 (54.87%), Postives = 481/678 (70.94%), Query Frame = 0

Query: 21  PPPSA---------AQFPFPETTSTAVPDPTKFFSPSLLSSPLPTNSFFQNFVLNNGDSP 80
           PPPS            F FP + S+ +PDP++FFS  LLSSPLPTNSFFQNF LNNGD  
Sbjct: 39  PPPSQQMSSSRQKNTPFLFPRSDSSVLPDPSRFFSHDLLSSPLPTNSFFQNFTLNNGDQA 98

Query: 81  EYIHPYLMRIANSSLSVSYPSRTSNSSITQLPVFPDLTISALNRASPVSNRTHFVSSFSD 140
           EY HPY+++ + SSLS+SYPS + NS+        D+TI+  +   P S ++H +SSFSD
Sbjct: 99  EYFHPYIIKPSTSSLSISYPSLSHNSAFIYEAFNADITITGSDGPDPHSRKSHLISSFSD 158

Query: 141 LGVDLDI--GDFRFHLVRGSPYLTFSVLKTSSVSISTGYGVRSVDSSGDSTKHVVRLSNG 200
           LGV LD    + RF LVRGSP++TFSV   SS++IST + V S+  +  STK+ V+L+N 
Sbjct: 159 LGVTLDFPSSNLRFFLVRGSPFITFSV--NSSITISTIHAVLSLSGNTSSTKYTVKLNNN 218

Query: 201 RNWVVYSTSAIGLIKSKNNEIISG-GFSGVIRVAVLPDSKSESEKILDLYRGCYPVSGFA 260
           + W++Y++S I L K   + I  G GFSG+IR+ VLP+     E ILD +   YPVSG A
Sbjct: 219 QTWLIYASSPINLTKDGVSSINCGDGFSGIIRIVVLPNPNPYFETILDGFSCSYPVSGDA 278

Query: 261 KLSENYGFQYKWQKKGSGDLLMLAHPLHRRILPGNQ---SVLQNLRYGSIDGDLVGVVGD 320
             ++ +  +YKW+K+G GDLLMLAHPLH ++L  N    +VL N +Y SIDGDLVGV+GD
Sbjct: 279 DFTKPFALEYKWEKRGYGDLLMLAHPLHLKLLSTNDCSITVLDNFKYNSIDGDLVGVIGD 338

Query: 321 SWDLNFNPIPVTWHSINGINNKFFPEIVAALKKDVSILNVTDLSSTPASYFYGKLLARAA 380
           SW L  +P+ VTWHSI G+      EI++AL KDV+ L+ +    T +SYFY KL+ARAA
Sbjct: 339 SWVLKPDPVSVTWHSIKGVQEDSHQEIISALIKDVNALD-SSAEVTNSSYFYAKLIARAA 398

Query: 381 RLALIAEEVNFAGDVVPAVVKFLKNGIQPWLDGKFAKNGFLFERKWAGLVTKNGATSQTE 440
           RLALIAEEV +  DV+P +  +LKN I+PWL+G F  NGFL++ KW G++TK G+     
Sbjct: 399 RLALIAEEVCYL-DVIPKIRTYLKNMIEPWLNGSFGPNGFLYDPKWGGVITKLGSRDSGA 458

Query: 441 DFGFGIYNDHHFHLGYYVYSIAVLAKLDPNWGNQYKSKAYALVNDYMNFIPKTSQFSIPF 500
           DFGFGIYNDHH+HLGY+VY+IAVLAK+DP WG +Y+ +AY L+ DY+    K ++ +  +
Sbjct: 459 DFGFGIYNDHHYHLGYFVYAIAVLAKIDPLWGKRYRPQAYTLMADYLTLGKKGAKSNSNY 518

Query: 501 ---RNFDFWKLHSWAAGLTEFPDGRNQESTSEAVNAYYAAALMGMAYGDKALTDAAAALT 560
              R FD +KLHSWA GLTEF DGRNQESTSEAVNAYY+AAL+G+AYGD  L  AA+ + 
Sbjct: 519 PRLRCFDLFKLHSWAGGLTEFADGRNQESTSEAVNAYYSAALLGLAYGDTHLVAAASMVL 578

Query: 561 AAEIAAAQTWWHVKKEGSIYDQGFAGENRVVGILWSAARESRLWFAPPEWRECRVGIQVL 620
             EI AA+ WW VK++ +IY Q F  ENRVVG+LWS  R+S LWFAP EW+ECR+GIQ+L
Sbjct: 579 TLEIHAAKMWWQVKEDDAIYPQDFTSENRVVGVLWSTKRDSGLWFAPKEWKECRLGIQLL 638

Query: 621 PVLPVTEKVFSDEEFVRELVEWVAPALEREDAGEGWKGFAYALEGIYDWKSGVEKIKKLK 680
           P+LPV+E +FSD  FV++LV W  PAL R+  GEGWKGF YALE +YD    +EKIK L 
Sbjct: 639 PLLPVSEVLFSDVTFVKQLVNWTMPALARDSVGEGWKGFVYALESMYDKDGAMEKIKGLN 698

BLAST of Sed0027875 vs. TAIR 10
Match: AT1G18310.1 (glycosyl hydrolase family 81 protein )

HSP 1 Score: 690.6 bits (1781), Expect = 1.2e-198
Identity = 355/687 (51.67%), Postives = 449/687 (65.36%), Query Frame = 0

Query: 24  SAAQFPFPETTSTAVPDPTKFFSPSLLSSPLPTNSFFQNFVLNNGDSPEYIHPYLMRIAN 83
           S A F FPET S+ +PDP++FFSP LLS+PLPTNSFFQNF L NGD  EY HPYL++  +
Sbjct: 4   SNALFLFPETKSSVLPDPSRFFSPDLLSNPLPTNSFFQNFTLKNGDQAEYFHPYLIKSVD 63

Query: 84  SSLSVSYPSRTSNSSITQLPVFPDLTISALNRASPVSNRTHFVSSFSDLGVDLDI--GDF 143
           SSL +SYPS   NS         D+ IS  N   P S +TH +SSFSDL V LD    + 
Sbjct: 64  SSLCISYPSLFHNSDFFYEVFTADIIISGSNGPGPRSRKTHIISSFSDLVVTLDFPSSNL 123

Query: 144 RFHLVRGSPYLTFSVLKTSSVSISTGYGVRSVDSSGDSTKHVVRLSNGRNWVVYSTSAIG 203
           RF LVRGSP                            STK   +L+N + W++Y++S I 
Sbjct: 124 RFFLVRGSP--------------------------SSSTKWSDKLTNNQRWLIYASSPID 183

Query: 204 LIKSKNNEI-ISGGFSGVIRVAVLPDSKSESEKILDLYRGCYPVSGFAKLSENYGFQYKW 263
           L K  ++ I   GGF+G++R+ VLP S    E  LD +  CYPVSG A  ++ +  +Y W
Sbjct: 184 LTKHGDSSIHCRGGFTGIVRITVLPGSNPGYESTLDRFSSCYPVSGDADFTKPFTLKYMW 243

Query: 264 QKKGSGDLLMLAHPLHRRILPGNQS-----------------------VLQNLRYGSIDG 323
           +K+GSGDLLMLAHPLH ++L  + S                       VL + RY SIDG
Sbjct: 244 EKRGSGDLLMLAHPLHLKLLAKDASSLFQQSTLLLVCPDRGCTNSSVTVLDHFRYKSIDG 303

Query: 324 DLVGVVGDSWDLNFNPIPVTWHSINGINNKFFPEIVAALKKDVSILNVTDLSSTPASYFY 383
           DLVGVVGDSW L  + + VTWHS+ G+    + EI++AL KDV+ LN +    T +SYFY
Sbjct: 304 DLVGVVGDSWVLKPDSVSVTWHSMKGVKKDSYKEIISALGKDVNGLN-SSAEVTSSSYFY 363

Query: 384 GKLLARAARLALIAEEVNFAGDVVPAVVKFLKNGIQPWLDGKFAKNGFLFERKWAGLVTK 443
           GKL+ARAAR ALIAEEV +  DV+P +V +LKN I+PWLDG F  NGFL++ KW GL+TK
Sbjct: 364 GKLIARAARFALIAEEVCYL-DVIPKIVTYLKNMIEPWLDGSFKPNGFLYDPKWGGLITK 423

Query: 444 NGATSQTEDFGFGIYNDHHFHLGYYVYSIAVLAKLDPNWGNQYKSKAYALVNDYMNFIPK 503
            G+     DFGFGIYNDHH+H+GY++Y+IAVLAK DP WG +Y+++AY+L+ D+M F  K
Sbjct: 424 QGSKDSQADFGFGIYNDHHYHIGYFLYAIAVLAKFDPLWGERYRAQAYSLLADFMTFGRK 483

Query: 504 ---TSQFSIP-FRNFDFWKLHSWAAGLTEFPDGRNQESTSEAVNAYYAAALMGMAYGDKA 563
               S  S P  RNFD +KLHSWA GLTEF DGRNQESTSEAVNAYY+AAL+G+AYGDK 
Sbjct: 484 DDNNSNSSYPRLRNFDLFKLHSWAGGLTEFWDGRNQESTSEAVNAYYSAALLGLAYGDKH 543

Query: 564 LTDAAAALTAAEIAAAQTWWHVKKEGSIYDQGFAGENRVVGILWSAARESRLWFAPPEWR 623
           L + A+ +   EI AA+ WW VKK  ++Y + F  ENRVVG+LWS  R+S LWF P EW+
Sbjct: 544 LVETASTIMTLEIHAAKMWWQVKKGEALYPKDFTAENRVVGVLWSTKRDSSLWFGPKEWK 603

Query: 624 ECRVGIQVLPVLPVTEKVFSDEEFVRELVEWVAPALEREDAGEGWKGFAYALEGIYDWKS 681
           ECR+GIQ+LP+LP              LV W  PAL+R   GEGWKGF YALE +YD   
Sbjct: 604 ECRLGIQLLPILP--------------LVNWTLPALQRNGVGEGWKGFLYALESLYDKDG 648

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038889504.10.0e+0082.15putative endo-1,3(4)-beta-glucanase 2 [Benincasa hispida][more]
KAG7025154.10.0e+0081.36hypothetical protein SDJN02_13977, partial [Cucurbita argyrosperma subsp. argyro... [more]
KAG6592332.10.0e+0081.39hypothetical protein SDJN03_14678, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_022925518.10.0e+0080.98probable endo-1,3(4)-beta-glucanase ARB_01444 [Cucurbita moschata][more]
XP_023534896.10.0e+0080.64probable endo-1,3(4)-beta-glucanase ARB_01444 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
D4AZ242.8e-4627.64Probable endo-1,3(4)-beta-glucanase ARB_01444 OS=Arthroderma benhamiae (strain A... [more]
Q098504.3e-3925.21Ascus wall endo-1,3(4)-beta-glucanase OS=Schizosaccharomyces pombe (strain 972 /... [more]
Q121682.1e-3825.15Endo-1,3(4)-beta-glucanase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S... [more]
P537535.8e-3625.18Endo-1,3(4)-beta-glucanase 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S... [more]
Q9UT451.3e-3025.20Primary septum endo-1,3(4)-beta-glucanase OS=Schizosaccharomyces pombe (strain 9... [more]
Match NameE-valueIdentityDescription
A0A6J1EFF10.0e+0080.98Endo-1,3(4)-beta-glucanase OS=Cucurbita moschata OX=3662 GN=LOC111432794 PE=3 SV... [more]
A0A6J1I8S40.0e+0080.78Endo-1,3(4)-beta-glucanase OS=Cucurbita maxima OX=3661 GN=LOC111472032 PE=3 SV=1[more]
A0A6J1DY720.0e+0079.86Endo-1,3(4)-beta-glucanase OS=Momordica charantia OX=3673 GN=LOC111025628 PE=3 S... [more]
A0A5A7TDJ60.0e+0081.10Endo-1,3(4)-beta-glucanase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaff... [more]
A0A142BTQ90.0e+0081.67Endo-1,3(4)-beta-glucanase OS=Cucumis melo OX=3656 GN=glu2 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
AT5G15870.12.3e-21354.87glycosyl hydrolase family 81 protein [more]
AT1G18310.11.2e-19851.67glycosyl hydrolase family 81 protein [more]
InterPro
Analysis Name: InterPro Annotations of Chayote (edule) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR040451Glycosyl hydrolase family 81, N-terminalPFAMPF03639Glyco_hydro_81coord: 50..319
e-value: 4.8E-58
score: 196.8
NoneNo IPR availableGENE3D2.70.98.30coord: 44..237
e-value: 5.3E-38
score: 132.8
NoneNo IPR availablePANTHERPTHR31983:SF18ENDO-1,3(4)-BETA-GLUCANASE-RELATEDcoord: 26..681
IPR040720Glycosyl hydrolase family 81, C-terminal domainPFAMPF17652Glyco_hydro81Ccoord: 326..676
e-value: 3.4E-98
score: 329.1
IPR005200Endo-1,3(4)-beta-glucanasePANTHERPTHR31983ENDO-1,3(4)-BETA-GLUCANASE 1coord: 26..681

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sed0027875.1Sed0027875.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008152 metabolic process
cellular_component GO:0005794 Golgi apparatus
cellular_component GO:0016020 membrane
molecular_function GO:0008422 beta-glucosidase activity
molecular_function GO:0030246 carbohydrate binding
molecular_function GO:0052861 glucan endo-1,3-beta-glucanase activity, C-3 substituted reducing group
molecular_function GO:0052862 glucan endo-1,4-beta-glucanase activity, C-3 substituted reducing group
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0000166 nucleotide binding
molecular_function GO:0016740 transferase activity