Sed0024783 (gene) Chayote v1

Overview
NameSed0024783
Typegene
OrganismSechium edule (Chayote v1)
DescriptionMannan endo-1,4-beta-mannosidase
LocationLG03: 210098 .. 212087 (-)
RNA-Seq ExpressionSed0024783
SyntenySed0024783
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTGTGTTTATAATTACACAATGATGATGATGATCAGCTGTTCTAAACTCATATTTGTGGTTGCTGTTTTTATTTTCTCTTATGTTGAGGGGGATAATCATCATTTTGATTTTTGGGTCGAGGATGCTTATACTCTCAATAATAATTTCTCCTACTCACACATGAGCCACCAGGGGTAACACTTCTAACTTTTGTATCCTAATTCTCTCGGTCCCACTATTCTGATCTCTTCCTTCAATCAAAACATGCAACATCAATTCATTCAATCAACATAATAATCTCTTGCGCTCTTCTTATTTCATTTTTATCTTTGAACATTTAGATGTTTTAGTTTAAATCCTCATGTGGTAGTTTAGTCGTGTATTCTCATTTTTGTGCCAATTTAACAGTAGAATGAAAGTTGATGAATATTTACAGGTTTGATGGAGTGGTTGAAAAGGATGGTGATGAATGGAGTATGGTGGAGACAAGGCAGAACCAATTTGTAGTCGAGGGCCGACCGTTGTATGTTAACGGCTTCAACACATACTGGTTAATGGTATTTGCAGCGGATGAATCCACAAGAGGAAAAGTTACAGAGGTGTTTAAGCAAGCTGCTTCTGTTGGTTTAACAGTTTGTAGGACTTGGGCCTTCAATGATGGCCAATGGAGAGCCCTTCAAAAGTCTCCATCTGTGTATGATGAGCAAGTCTTCAAGGTCTACGAATTTCCCACCTTCATCATCTTTTTTTGGTCTCGTGTTCTGCAAGCTTGATTTTTTAAATACAAAAAACATGGCAGGGATTAGATTTTGTGATTAGTGAAGCAAAGAAGTTTAAGATAAGGGTGATATTATCATTAGCAAACAACTGGGAAGCATATGGTGGCAAATCACAATATGTGAAATGGGGGAAAGCTGCCGGCCTCAACTTCACATCTGATGATGATTTCTTCTCTGATCCAACTCTCAGAACCTACTACAAGGCTCATGTTCATGTGACCTCCCAACATTCTTCATTCTCAATTATTACTAAACATATCAACTTATATTTGTGTTTCTTTTTCCCATGTGAATGGTATAGACGGTGCTTAACAGGGTGAATACATTCACAAATGTAACGTACAAGGAAGATCCGACCATTTTCGCCTGGGAATTGATGAACGAACCGCGTTGCCCATCAGACCCCTCCGGTGATAAACTTCAGGTTTGTCTTCCTTTAACTGCCATGCCACAGAGTTGATATCATGAAAAGAACAAGATGTAAATTGAGTTCCTGAATGGCATTGGCAGGGATGGATTCAAGAAATGGCTGTGTATGTGAAGAGCATGGACCCAAAGCATTTAGTGGAGGTTGGTTTGGAGGGATTTTATGGCCCATCCACACCCGGTAGAGTTCAGTTCAATCCAAATACATATGCACAGCAAGTTGGAACTGATTTCATCAGAAACCATAAGGCTCTTGGTGTTGATTTTGCTTCAGTTCACATATATGCAGACACATGGTAGGCTTCTACATCGCCCAAACATTTAGTAAATATGATACAATGGCTCTAACATGAACTCATTGCCAAAGGGTTTCTCAAACGGTCTCAGATGCCCAACTCCGATTCACAAAATCATGGATGCAGGCCCACATCGAGGATGCAGAGAAGTACCTTGGAATGCCAGTTATATTCACAGAGTTTGGAGTATCGTCGAAAGATCCCGGATACAACTTGAGTTTCAGAGATGCAGTTATTAACTCAGTGTACAAGACATTATTGGACTCAACAAAGAAAGGAGGGAGTGGAGGAGGAGGCTTGGTGTGGCAGCTATTCCCAGAAGGAACTGAGTACATGGATGATGGCTATGCAATTGTGCTCTCCAAATGTCCTTCAACTTCCAACATTATAGCTCTTCAATCCACAATCTTGTCCATCCTAAACTCCATTTGTTCCATGACATGTCGGTGGGGTTGTAAGAACAAAAATGCTATGGATCTCATCTTTCATCATGAGATTCACATGTAA

mRNA sequence

ATGTGTGTTTATAATTACACAATGATGATGATGATCAGCTGTTCTAAACTCATATTTGTGGTTGCTGTTTTTATTTTCTCTTATGTTGAGGGGGATAATCATCATTTTGATTTTTGGGTCGAGGATGCTTATACTCTCAATAATAATTTCTCCTACTCACACATGAGCCACCAGGGGTTTGATGGAGTGGTTGAAAAGGATGGTGATGAATGGAGTATGGTGGAGACAAGGCAGAACCAATTTGTAGTCGAGGGCCGACCGTTGTATGTTAACGGCTTCAACACATACTGGTTAATGGTATTTGCAGCGGATGAATCCACAAGAGGAAAAGTTACAGAGGTGTTTAAGCAAGCTGCTTCTGTTGGTTTAACAGTTTGTAGGACTTGGGCCTTCAATGATGGCCAATGGAGAGCCCTTCAAAAGTCTCCATCTGTGTATGATGAGCAAGTCTTCAAGGGATTAGATTTTGTGATTAGTGAAGCAAAGAAGTTTAAGATAAGGGTGATATTATCATTAGCAAACAACTGGGAAGCATATGGTGGCAAATCACAATATGTGAAATGGGGGAAAGCTGCCGGCCTCAACTTCACATCTGATGATGATTTCTTCTCTGATCCAACTCTCAGAACCTACTACAAGGCTCATGTTCATACGGTGCTTAACAGGGTGAATACATTCACAAATGTAACGTACAAGGAAGATCCGACCATTTTCGCCTGGGAATTGATGAACGAACCGCGTTGCCCATCAGACCCCTCCGGTGATAAACTTCAGGGATGGATTCAAGAAATGGCTGTGTATGTGAAGAGCATGGACCCAAAGCATTTAGTGGAGGTTGGTTTGGAGGGATTTTATGGCCCATCCACACCCGGTAGAGTTCAGTTCAATCCAAATACATATGCACAGCAAGTTGGAACTGATTTCATCAGAAACCATAAGGCTCTTGGTGTTGATTTTGCTTCAGTTCACATATATGCAGACACATGGGTTTCTCAAACGGTCTCAGATGCCCAACTCCGATTCACAAAATCATGGATGCAGGCCCACATCGAGGATGCAGAGAAGTACCTTGGAATGCCAGTTATATTCACAGAGTTTGGAGTATCGTCGAAAGATCCCGGATACAACTTGAGTTTCAGAGATGCAGTTATTAACTCAGTGTACAAGACATTATTGGACTCAACAAAGAAAGGAGGGAGTGGAGGAGGAGGCTTGGTGTGGCAGCTATTCCCAGAAGGAACTGAGTACATGGATGATGGCTATGCAATTGTGCTCTCCAAATGTCCTTCAACTTCCAACATTATAGCTCTTCAATCCACAATCTTGTCCATCCTAAACTCCATTTGTTCCATGACATGTCGGTGGGGTTGTAAGAACAAAAATGCTATGGATCTCATCTTTCATCATGAGATTCACATGTAA

Coding sequence (CDS)

ATGTGTGTTTATAATTACACAATGATGATGATGATCAGCTGTTCTAAACTCATATTTGTGGTTGCTGTTTTTATTTTCTCTTATGTTGAGGGGGATAATCATCATTTTGATTTTTGGGTCGAGGATGCTTATACTCTCAATAATAATTTCTCCTACTCACACATGAGCCACCAGGGGTTTGATGGAGTGGTTGAAAAGGATGGTGATGAATGGAGTATGGTGGAGACAAGGCAGAACCAATTTGTAGTCGAGGGCCGACCGTTGTATGTTAACGGCTTCAACACATACTGGTTAATGGTATTTGCAGCGGATGAATCCACAAGAGGAAAAGTTACAGAGGTGTTTAAGCAAGCTGCTTCTGTTGGTTTAACAGTTTGTAGGACTTGGGCCTTCAATGATGGCCAATGGAGAGCCCTTCAAAAGTCTCCATCTGTGTATGATGAGCAAGTCTTCAAGGGATTAGATTTTGTGATTAGTGAAGCAAAGAAGTTTAAGATAAGGGTGATATTATCATTAGCAAACAACTGGGAAGCATATGGTGGCAAATCACAATATGTGAAATGGGGGAAAGCTGCCGGCCTCAACTTCACATCTGATGATGATTTCTTCTCTGATCCAACTCTCAGAACCTACTACAAGGCTCATGTTCATACGGTGCTTAACAGGGTGAATACATTCACAAATGTAACGTACAAGGAAGATCCGACCATTTTCGCCTGGGAATTGATGAACGAACCGCGTTGCCCATCAGACCCCTCCGGTGATAAACTTCAGGGATGGATTCAAGAAATGGCTGTGTATGTGAAGAGCATGGACCCAAAGCATTTAGTGGAGGTTGGTTTGGAGGGATTTTATGGCCCATCCACACCCGGTAGAGTTCAGTTCAATCCAAATACATATGCACAGCAAGTTGGAACTGATTTCATCAGAAACCATAAGGCTCTTGGTGTTGATTTTGCTTCAGTTCACATATATGCAGACACATGGGTTTCTCAAACGGTCTCAGATGCCCAACTCCGATTCACAAAATCATGGATGCAGGCCCACATCGAGGATGCAGAGAAGTACCTTGGAATGCCAGTTATATTCACAGAGTTTGGAGTATCGTCGAAAGATCCCGGATACAACTTGAGTTTCAGAGATGCAGTTATTAACTCAGTGTACAAGACATTATTGGACTCAACAAAGAAAGGAGGGAGTGGAGGAGGAGGCTTGGTGTGGCAGCTATTCCCAGAAGGAACTGAGTACATGGATGATGGCTATGCAATTGTGCTCTCCAAATGTCCTTCAACTTCCAACATTATAGCTCTTCAATCCACAATCTTGTCCATCCTAAACTCCATTTGTTCCATGACATGTCGGTGGGGTTGTAAGAACAAAAATGCTATGGATCTCATCTTTCATCATGAGATTCACATGTAA

Protein sequence

MCVYNYTMMMMISCSKLIFVVAVFIFSYVEGDNHHFDFWVEDAYTLNNNFSYSHMSHQGFDGVVEKDGDEWSMVETRQNQFVVEGRPLYVNGFNTYWLMVFAADESTRGKVTEVFKQAASVGLTVCRTWAFNDGQWRALQKSPSVYDEQVFKGLDFVISEAKKFKIRVILSLANNWEAYGGKSQYVKWGKAAGLNFTSDDDFFSDPTLRTYYKAHVHTVLNRVNTFTNVTYKEDPTIFAWELMNEPRCPSDPSGDKLQGWIQEMAVYVKSMDPKHLVEVGLEGFYGPSTPGRVQFNPNTYAQQVGTDFIRNHKALGVDFASVHIYADTWVSQTVSDAQLRFTKSWMQAHIEDAEKYLGMPVIFTEFGVSSKDPGYNLSFRDAVINSVYKTLLDSTKKGGSGGGGLVWQLFPEGTEYMDDGYAIVLSKCPSTSNIIALQSTILSILNSICSMTCRWGCKNKNAMDLIFHHEIHM
Homology
BLAST of Sed0024783 vs. NCBI nr
Match: XP_038886206.1 (mannan endo-1,4-beta-mannosidase 6 isoform X1 [Benincasa hispida])

HSP 1 Score: 801.6 bits (2069), Expect = 3.7e-228
Identity = 386/466 (82.83%), Postives = 421/466 (90.34%), Query Frame = 0

Query: 11  MISCSKLIFVVAVFIF-------SYVE--GDNHHFDFWVEDAYTLNNNFSYSHMSHQGFD 70
           M+   KLIF++ VF+F       S+VE  GD+  FD  VEDA    N+F+YS MSH GFD
Sbjct: 6   MLLSLKLIFLIVVFMFCVEYTVSSFVEGDGDDQPFDISVEDA--TENHFTYSDMSH-GFD 65

Query: 71  GVVEKDGDEWSMVETRQNQFVVEGRPLYVNGFNTYWLMVFAADESTRGKVTEVFKQAASV 130
           G+V+KD D WSMVETRQNQFVV+G+P YVNGFNTYWLM+FAAD+STRGKVTE+FKQAASV
Sbjct: 66  GLVQKDDDAWSMVETRQNQFVVDGQPFYVNGFNTYWLMIFAADQSTRGKVTELFKQAASV 125

Query: 131 GLTVCRTWAFNDGQWRALQKSPSVYDEQVFKGLDFVISEAKKFKIRVILSLANNWEAYGG 190
           GLTVCRTWAFNDGQWRALQKSPSVYDE+VFKGLDFVISEAKK+KIR+ILSLANNWEAYGG
Sbjct: 126 GLTVCRTWAFNDGQWRALQKSPSVYDEEVFKGLDFVISEAKKYKIRLILSLANNWEAYGG 185

Query: 191 KSQYVKWGKAAGLNFTSDDDFFSDPTLRTYYKAHVHTVLNRVNTFTNVTYKEDPTIFAWE 250
           K+QYVKWGKAAGLN TSDDDFFSDPTLR+YYKAHV TVLNRVN+FTNV YKEDPTIFAWE
Sbjct: 186 KAQYVKWGKAAGLNLTSDDDFFSDPTLRSYYKAHVKTVLNRVNSFTNVPYKEDPTIFAWE 245

Query: 251 LMNEPRCPSDPSGDKLQGWIQEMAVYVKSMDPKHLVEVGLEGFYGPSTPGRVQFNPNTYA 310
           LMNEPRC SDPSGDKLQGWIQEMAV+VKSMDPKHL+EVGLEGFYGPSTP RVQFNPNTYA
Sbjct: 246 LMNEPRCTSDPSGDKLQGWIQEMAVFVKSMDPKHLLEVGLEGFYGPSTPNRVQFNPNTYA 305

Query: 311 QQVGTDFIRNHKALGVDFASVHIYADTWVSQTVSDAQLRFTKSWMQAHIEDAEKYLGMPV 370
           QQVGTDFIRNHK LGVDFASVHIYADTWVSQT+SD  L+FTKSWM+AHIEDAEKYLGMPV
Sbjct: 306 QQVGTDFIRNHKVLGVDFASVHIYADTWVSQTISDTHLQFTKSWMEAHIEDAEKYLGMPV 365

Query: 371 IFTEFGVSSKDPGYNLSFRDAVINSVYKTLLDSTKKGGSGGGGLVWQLFPEGTEYMDDGY 430
           +F EFGVSS+DPGYN ++RD V+NSVYKTLLDSTKKGGSGGG LVWQLFPEGT+YMDDGY
Sbjct: 366 LFAEFGVSSRDPGYNSTYRDTVLNSVYKTLLDSTKKGGSGGGSLVWQLFPEGTDYMDDGY 425

Query: 431 AIVLSKCPSTSNIIALQSTILSILNSICSMTCRWGCKNKNAMDLIF 468
           AIVLSK PSTSNII+L ST +SI NSICSM CRWGCK KN MD+IF
Sbjct: 426 AIVLSKFPSTSNIISLHSTRMSIFNSICSMRCRWGCKKKNVMDIIF 468

BLAST of Sed0024783 vs. NCBI nr
Match: XP_023540740.1 (mannan endo-1,4-beta-mannosidase 6-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 801.2 bits (2068), Expect = 4.8e-228
Identity = 390/472 (82.63%), Postives = 424/472 (89.83%), Query Frame = 0

Query: 5   NYTMMMMISCSKLIFVVAVFIFSYVEGD----NHHFDFWVEDAYTLNNNFSYSHMSHQGF 64
           +YT++ +    KL+F++AVF+F+ VEGD    N  FD  VED        + +H+SH GF
Sbjct: 4   HYTILSL----KLVFLIAVFVFT-VEGDGVGNNQQFDLLVEDT-------TENHLSH-GF 63

Query: 65  DGVVEKDGDEWSMVETRQNQFVVEGRPLYVNGFNTYWLMVFAADESTRGKVTEVFKQAAS 124
           DGVVEK+ D WSMVETRQNQFVV+G+P YVNGFNTYWLM+FAAD+STRGKVTE+FKQAAS
Sbjct: 64  DGVVEKEDDPWSMVETRQNQFVVDGQPFYVNGFNTYWLMIFAADQSTRGKVTELFKQAAS 123

Query: 125 VGLTVCRTWAFNDGQWRALQKSPSVYDEQVFKGLDFVISEAKKFKIRVILSLANNWEAYG 184
           VGLTVCRTWAFNDGQWRALQKSPSVYDE+VFKGLDFVISEAKK+KIR+ILSLANNWEAYG
Sbjct: 124 VGLTVCRTWAFNDGQWRALQKSPSVYDEEVFKGLDFVISEAKKYKIRLILSLANNWEAYG 183

Query: 185 GKSQYVKWGKAAGLNFTSDDDFFSDPTLRTYYKAHVHTVLNRVNTFTNVTYKEDPTIFAW 244
           GK+QYVKWGKAAGLN TSDDDFFSD TLR+YYKAHV TVLNRVN+FTNV YK+DPTIFAW
Sbjct: 184 GKAQYVKWGKAAGLNLTSDDDFFSDSTLRSYYKAHVKTVLNRVNSFTNVMYKDDPTIFAW 243

Query: 245 ELMNEPRCPSDPSGDKLQGWIQEMAVYVKSMDPKHLVEVGLEGFYGPSTPGRVQFNPNTY 304
           ELMNEPRC SDPSGDKLQGWIQEMAVYVKSMDPKHL+EVGLEGFYGPSTP RVQFNPNTY
Sbjct: 244 ELMNEPRCTSDPSGDKLQGWIQEMAVYVKSMDPKHLLEVGLEGFYGPSTPNRVQFNPNTY 303

Query: 305 AQQVGTDFIRNHKALGVDFASVHIYADTWVSQTVSDAQLRFTKSWMQAHIEDAEKYLGMP 364
           AQQVGTDFIRNHK LGVDFASVHIYADTWVSQT+SDA L+FTKSWM+AHIEDAEKYLGMP
Sbjct: 304 AQQVGTDFIRNHKVLGVDFASVHIYADTWVSQTISDAHLQFTKSWMEAHIEDAEKYLGMP 363

Query: 365 VIFTEFGVSSKDPGYNLSFRDAVINSVYKTLLDSTKKGGSGGGGLVWQLFPEGTEYMDDG 424
           VIF EFGVSSKDPGYN SFRDA INSVYKTLLDSTKKGGSGGG LVWQLFPEGTE MDDG
Sbjct: 364 VIFAEFGVSSKDPGYNSSFRDAFINSVYKTLLDSTKKGGSGGGSLVWQLFPEGTENMDDG 423

Query: 425 YAIVLSKCPSTSNIIALQSTILSILNSICSMTCRWGCKNKNAMDLIF--HHE 471
           YAIVLSK PSTSN+I LQST LS+ NS+CSM CRWGCK KNAMDL+F  HH+
Sbjct: 424 YAIVLSKSPSTSNVITLQSTRLSLFNSMCSMKCRWGCKKKNAMDLLFLGHHD 462

BLAST of Sed0024783 vs. NCBI nr
Match: XP_022990348.1 (mannan endo-1,4-beta-mannosidase 6-like [Cucurbita maxima])

HSP 1 Score: 801.2 bits (2068), Expect = 4.8e-228
Identity = 390/472 (82.63%), Postives = 425/472 (90.04%), Query Frame = 0

Query: 5   NYTMMMMISCSKLIFVVAVFIFSYVEGD----NHHFDFWVEDAYTLNNNFSYSHMSHQGF 64
           +YT++ +    KLIF++AVF+F+ VEGD    N  FD  VED        + +H+SH GF
Sbjct: 4   HYTILSL----KLIFLIAVFVFT-VEGDGVGNNQQFDLLVEDT-------TENHLSH-GF 63

Query: 65  DGVVEKDGDEWSMVETRQNQFVVEGRPLYVNGFNTYWLMVFAADESTRGKVTEVFKQAAS 124
           DGVVEK+ D WSMVETRQNQFVV+G+P YVNGFNTYWLM+FAAD+STRGKVT++FKQAAS
Sbjct: 64  DGVVEKEDDPWSMVETRQNQFVVDGQPFYVNGFNTYWLMIFAADQSTRGKVTDLFKQAAS 123

Query: 125 VGLTVCRTWAFNDGQWRALQKSPSVYDEQVFKGLDFVISEAKKFKIRVILSLANNWEAYG 184
           VGLTVCRTWAFNDGQWRALQKSPSVYDE+VFKGLDFVISEAKK+KIR+ILSLANNWEAYG
Sbjct: 124 VGLTVCRTWAFNDGQWRALQKSPSVYDEEVFKGLDFVISEAKKYKIRLILSLANNWEAYG 183

Query: 185 GKSQYVKWGKAAGLNFTSDDDFFSDPTLRTYYKAHVHTVLNRVNTFTNVTYKEDPTIFAW 244
           GK+QYVKWGKAAGLN TSDDDFFSD T+R+YYKAHV TVLNRVN+FTNV YK+DPTIFAW
Sbjct: 184 GKAQYVKWGKAAGLNLTSDDDFFSDSTVRSYYKAHVKTVLNRVNSFTNVMYKDDPTIFAW 243

Query: 245 ELMNEPRCPSDPSGDKLQGWIQEMAVYVKSMDPKHLVEVGLEGFYGPSTPGRVQFNPNTY 304
           ELMNEPRC SDPSGDKLQGWIQEMAVYVKSMDPKHL+EVGLEGFYGPSTP RVQFNPNTY
Sbjct: 244 ELMNEPRCTSDPSGDKLQGWIQEMAVYVKSMDPKHLLEVGLEGFYGPSTPNRVQFNPNTY 303

Query: 305 AQQVGTDFIRNHKALGVDFASVHIYADTWVSQTVSDAQLRFTKSWMQAHIEDAEKYLGMP 364
           AQQVGTDFIRNHK LGVDFASVHIYADTWVSQT+SDAQL+FTKSWM+AHIEDAEKYLGMP
Sbjct: 304 AQQVGTDFIRNHKVLGVDFASVHIYADTWVSQTISDAQLQFTKSWMEAHIEDAEKYLGMP 363

Query: 365 VIFTEFGVSSKDPGYNLSFRDAVINSVYKTLLDSTKKGGSGGGGLVWQLFPEGTEYMDDG 424
           VIF EFGVSSKDPGYN SFRDA INSVYKTLLDSTKKGGSGGG LVWQLFPEGTE MDDG
Sbjct: 364 VIFAEFGVSSKDPGYNSSFRDAFINSVYKTLLDSTKKGGSGGGSLVWQLFPEGTENMDDG 423

Query: 425 YAIVLSKCPSTSNIIALQSTILSILNSICSMTCRWGCKNKNAMDLIF--HHE 471
           YAIVLSK PSTSN+I LQST LS+ NS+CSM CRWGCK KNAMDL+F  HH+
Sbjct: 424 YAIVLSKSPSTSNVITLQSTRLSLFNSMCSMKCRWGCKKKNAMDLLFLGHHD 462

BLAST of Sed0024783 vs. NCBI nr
Match: XP_022954273.1 (mannan endo-1,4-beta-mannosidase 6-like [Cucurbita moschata] >KAG6602316.1 Mannan endo-1,4-beta-mannosidase 6, partial [Cucurbita argyrosperma subsp. sororia] >KAG7032999.1 Mannan endo-1,4-beta-mannosidase 6 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 798.1 bits (2060), Expect = 4.1e-227
Identity = 388/472 (82.20%), Postives = 424/472 (89.83%), Query Frame = 0

Query: 5   NYTMMMMISCSKLIFVVAVFIFSYVEGD----NHHFDFWVEDAYTLNNNFSYSHMSHQGF 64
           +YT++ +    KLIF++AVF+F+ VEGD    N  FD  VED        + +H+SH GF
Sbjct: 4   HYTILSL----KLIFLIAVFVFT-VEGDGVGNNQQFDLLVEDT-------TENHLSH-GF 63

Query: 65  DGVVEKDGDEWSMVETRQNQFVVEGRPLYVNGFNTYWLMVFAADESTRGKVTEVFKQAAS 124
           DGVVEK+ D WSMVETRQNQFVV+G+P YVNGFNTYWLM+FAAD+STRGKVTE+FKQAAS
Sbjct: 64  DGVVEKEDDPWSMVETRQNQFVVDGQPFYVNGFNTYWLMIFAADQSTRGKVTELFKQAAS 123

Query: 125 VGLTVCRTWAFNDGQWRALQKSPSVYDEQVFKGLDFVISEAKKFKIRVILSLANNWEAYG 184
           VGLTVCRTWAFNDGQWRALQKSPSVYDE+VFKGLDFVISEAKK+KIR+ILSLANNWEA+G
Sbjct: 124 VGLTVCRTWAFNDGQWRALQKSPSVYDEEVFKGLDFVISEAKKYKIRLILSLANNWEAFG 183

Query: 185 GKSQYVKWGKAAGLNFTSDDDFFSDPTLRTYYKAHVHTVLNRVNTFTNVTYKEDPTIFAW 244
           GK+QYVKWGKAAGLN TSDDDFFSD T+R+YYKAHV TVLNRVN+FTNV YK+DPTIFAW
Sbjct: 184 GKAQYVKWGKAAGLNLTSDDDFFSDSTVRSYYKAHVKTVLNRVNSFTNVMYKDDPTIFAW 243

Query: 245 ELMNEPRCPSDPSGDKLQGWIQEMAVYVKSMDPKHLVEVGLEGFYGPSTPGRVQFNPNTY 304
           ELMNEPRC SDPSGDKLQGWIQEMAVYVKSMDPKHL+EVGLEGFYGPSTP RVQFNPNTY
Sbjct: 244 ELMNEPRCTSDPSGDKLQGWIQEMAVYVKSMDPKHLLEVGLEGFYGPSTPNRVQFNPNTY 303

Query: 305 AQQVGTDFIRNHKALGVDFASVHIYADTWVSQTVSDAQLRFTKSWMQAHIEDAEKYLGMP 364
           AQQVGTDFIRNHK LGVDFASVHIYADTWVSQT+SDA L+FTKSWM+AHIEDAEKYLGMP
Sbjct: 304 AQQVGTDFIRNHKVLGVDFASVHIYADTWVSQTISDAHLQFTKSWMEAHIEDAEKYLGMP 363

Query: 365 VIFTEFGVSSKDPGYNLSFRDAVINSVYKTLLDSTKKGGSGGGGLVWQLFPEGTEYMDDG 424
           V+F EFGVSSKDPGYN SFRDA INSVYKTLLDSTKKGGSGGG LVWQLFPEGTE MDDG
Sbjct: 364 VLFAEFGVSSKDPGYNSSFRDAFINSVYKTLLDSTKKGGSGGGSLVWQLFPEGTENMDDG 423

Query: 425 YAIVLSKCPSTSNIIALQSTILSILNSICSMTCRWGCKNKNAMDLIF--HHE 471
           YAIVLSK PSTSN+I LQST LS+ NS+CSM CRWGCK KNAMDL+F  HH+
Sbjct: 424 YAIVLSKSPSTSNVITLQSTRLSLFNSMCSMKCRWGCKKKNAMDLLFLGHHD 462

BLAST of Sed0024783 vs. NCBI nr
Match: XP_022938707.1 (mannan endo-1,4-beta-mannosidase 6-like [Cucurbita moschata] >XP_022938708.1 mannan endo-1,4-beta-mannosidase 6-like [Cucurbita moschata])

HSP 1 Score: 788.1 bits (2034), Expect = 4.2e-224
Identity = 387/467 (82.87%), Postives = 415/467 (88.87%), Query Frame = 0

Query: 17  LIFVVAVFIF-------SYVE----GDNHHFDFWVEDAYTLNNNFSYSHMSHQGFDGVVE 76
           LI ++AVFIF       S+VE    GD+  FDF VED     N+F+YS MS+ GFDGVV+
Sbjct: 12  LIGLIAVFIFSVEHTVSSFVEGDGDGDDQQFDFSVED--VPENHFTYSDMSN-GFDGVVQ 71

Query: 77  KDGDEWSMVETRQNQFVVEGRPLYVNGFNTYWLMVFAADESTRGKVTEVFKQAASVGLTV 136
           K  D WSMVETRQNQFVV+G+P YVNGFNTYWLMVFAAD+STRGKVTE+FKQAASVGLTV
Sbjct: 72  KKNDAWSMVETRQNQFVVDGQPFYVNGFNTYWLMVFAADQSTRGKVTELFKQAASVGLTV 131

Query: 137 CRTWAFNDGQWRALQKSPSVYDEQVFKGLDFVISEAKKFKIRVILSLANNWEAYGGKSQY 196
           CRTWAFNDGQWRALQKSPSVYDE+VFK LDFVISEAKK+KIR+ILSLANNWEAYGGK+QY
Sbjct: 132 CRTWAFNDGQWRALQKSPSVYDEEVFKALDFVISEAKKYKIRLILSLANNWEAYGGKAQY 191

Query: 197 VKWGKAAGLNFTSDDDFFSDPTLRTYYKAHVHTVLNRVNTFTNVTYKEDPTIFAWELMNE 256
           VKWG+AAGLN TSDDDFFSDPTLR+YYKAHV TVLNRVNT+TNV YKEDPTIFAWELMNE
Sbjct: 192 VKWGRAAGLNLTSDDDFFSDPTLRSYYKAHVATVLNRVNTYTNVMYKEDPTIFAWELMNE 251

Query: 257 PRCPSDPSGDKLQGWIQEMAVYVKSMDPKHLVEVGLEGFYGPSTPGRVQFNPNTYAQQVG 316
           PRC SDPSGDKLQGWIQEMAV+VKSMDPKHLVEVGLEGFYGPSTP RVQ NPNTYAQQVG
Sbjct: 252 PRCTSDPSGDKLQGWIQEMAVFVKSMDPKHLVEVGLEGFYGPSTPNRVQLNPNTYAQQVG 311

Query: 317 TDFIRNHKALGVDFASVHIYADTWVSQTVSDAQLRFTKSWMQAHIEDAEKYLGMPVIFTE 376
           TDFIRNHK LGVDFASVHIYADTWVS +VSD  L+FTKSWM AHIEDAEKYLGMPVIF E
Sbjct: 312 TDFIRNHKVLGVDFASVHIYADTWVSPSVSDTHLQFTKSWMDAHIEDAEKYLGMPVIFAE 371

Query: 377 FGVSSKDPGYNLSFRDAVINSVYKTLLDSTKKGGSGGGGLVWQLFPEGTEYMDDGYAIVL 436
           FGVSSKDPGYN +FRDAV+N VYKTLL STKKGGSGGG LVWQLFP+GT+YMDDGYAIVL
Sbjct: 372 FGVSSKDPGYNSTFRDAVLNLVYKTLLGSTKKGGSGGGSLVWQLFPDGTDYMDDGYAIVL 431

Query: 437 SKCPSTSNIIALQSTILSILNSICSMTCRWGCKNKNAMDLIF--HHE 471
           SK PSTSNII+L ST LSI NSICSM CRWGCK KN MDLIF  HH+
Sbjct: 432 SKSPSTSNIISLHSTRLSIFNSICSMRCRWGCKKKNLMDLIFPDHHD 475

BLAST of Sed0024783 vs. ExPASy Swiss-Prot
Match: Q9LZV3 (Mannan endo-1,4-beta-mannosidase 6 OS=Arabidopsis thaliana OX=3702 GN=MAN6 PE=2 SV=1)

HSP 1 Score: 643.7 bits (1659), Expect = 1.7e-183
Identity = 292/428 (68.22%), Postives = 356/428 (83.18%), Query Frame = 0

Query: 46  LNNNFSYSHMSHQGFDGVVEKDGDE-WSMVETRQNQFVVEGRPLYVNGFNTYWLMVFAAD 105
           L  N + + +  +  +   E  G+E W MV+ +  QF + G+P YVNGFNTYW+M  AAD
Sbjct: 19  LTQNRALADLDSESHEVNSESVGEEQWEMVQRKGMQFTLNGQPFYVNGFNTYWMMTLAAD 78

Query: 106 ESTRGKVTEVFKQAASVGLTVCRTWAFNDGQWRALQKSPSVYDEQVFKGLDFVISEAKKF 165
            STRGKVTEVF+QA++VG+TV RTWAFNDGQWRALQKSPSVYDE+VFK LDFV+SEA+K+
Sbjct: 79  NSTRGKVTEVFQQASAVGMTVGRTWAFNDGQWRALQKSPSVYDEEVFKALDFVLSEARKY 138

Query: 166 KIRVILSLANNWEAYGGKSQYVKWGKAAGLNFTSDDDFFSDPTLRTYYKAHVHTVLNRVN 225
           KIR+ILSL NNW+AYGGK+QYVKWG A+GLN TSDDDFF++PTLR +Y++HV TVLNRVN
Sbjct: 139 KIRLILSLVNNWDAYGGKAQYVKWGNASGLNLTSDDDFFTNPTLRNFYQSHVRTVLNRVN 198

Query: 226 TFTNVTYKEDPTIFAWELMNEPRCPSDPSGDKLQGWIQEMAVYVKSMDPKHLVEVGLEGF 285
           TFTN+TYK DPTIFAWELMNEPRCPSDPSGDKLQ WIQEMAV+VKS+D KHLVE+GLEGF
Sbjct: 199 TFTNITYKNDPTIFAWELMNEPRCPSDPSGDKLQSWIQEMAVFVKSLDAKHLVEIGLEGF 258

Query: 286 YGPSTPGRVQFNPNTYAQQVGTDFIRNHKALGVDFASVHIYADTWVSQTVSDAQLRFTKS 345
           YGPS P R +FNPN YA QVGTDFIRN++ LG+DFASVH+Y D+W+S  VS++ L FT S
Sbjct: 259 YGPSAPARTRFNPNPYAAQVGTDFIRNNQVLGIDFASVHVYPDSWISPAVSNSFLEFTSS 318

Query: 346 WMQAHIEDAEKYLGMPVIFTEFGVSSKDPGYNLSFRDAVINSVYKTLLDSTKKGGSGGGG 405
           WMQAH+EDAE YLGMPV+FTEFGVS+ DPG+N SFRD ++N+VYK  L+ST+KGG+G G 
Sbjct: 319 WMQAHVEDAEMYLGMPVLFTEFGVSAHDPGFNTSFRDMMLNTVYKMTLNSTRKGGAGAGS 378

Query: 406 LVWQLFPEGTEYMDDGYAIVLSKCPSTSNIIALQSTILSILNSICSMTCRWGCKNKN--A 465
           LVWQ+FP+G E+MDDGYA+ L++  + S II+LQS  L+I NS+CS  CRWGCK KN  A
Sbjct: 379 LVWQVFPQGAEFMDDGYAVYLTRAHTASKIISLQSKRLAIFNSLCSWRCRWGCKKKNQTA 438

Query: 466 MDLIFHHE 471
           +D +  H+
Sbjct: 439 LDALLSHD 446

BLAST of Sed0024783 vs. ExPASy Swiss-Prot
Match: Q5W6G0 (Putative mannan endo-1,4-beta-mannosidase 5 OS=Oryza sativa subsp. japonica OX=39947 GN=MAN5 PE=2 SV=2)

HSP 1 Score: 529.3 bits (1362), Expect = 4.6e-149
Identity = 255/421 (60.57%), Postives = 310/421 (73.63%), Query Frame = 0

Query: 68  GDEWSMVETRQNQFVV-EGRPLYVNGFNTYWLMVFAADESTRGKVTEVFKQAASVGLTVC 127
           G EW MV TR  QFVV  GRP YVNGFNTYWLMV A D STRGKVTEVF+QAA+VGLTVC
Sbjct: 72  GGEWGMVRTRGAQFVVGGGRPFYVNGFNTYWLMVLAVDPSTRGKVTEVFRQAAAVGLTVC 131

Query: 128 RTWAFNDGQWRALQKSPSVYDEQVFKGLDFVISEAKKFKIRVILSLANNWEAYGGKSQYV 187
           RTWAFNDG WRALQKSP VYDE+VFK LDFV+SEA+K KIR+IL L NNW+ YGGK+QYV
Sbjct: 132 RTWAFNDGGWRALQKSPGVYDEEVFKALDFVVSEARKHKIRLILPLINNWDDYGGKAQYV 191

Query: 188 KWGKAAGLNFTSDDDFFSDPTLRTYYKAHVHTVLNRVNTFTNVTYKEDPTIFAWELMNEP 247
           +W +AA       D FFSD T+R Y+K+HV  VL RVN +T V Y++DPTI AWELMNEP
Sbjct: 192 RWAQAAAAG-AGADAFFSDETVRGYFKSHVTAVLTRVNAYTGVAYRDDPTIMAWELMNEP 251

Query: 248 RCPSDPSGDKLQGWIQEMAVYVKSMDPKHLVEVGLEGFYGPST-PGRVQFNPNTYAQQVG 307
           RC SDP+GD LQ WI EMA +VKS+DP HL+ VG EGFYGPS+ P R++ NPN      G
Sbjct: 252 RCASDPTGDTLQAWIAEMAFHVKSVDPAHLLGVGAEGFYGPSSPPARLRVNPNADVALAG 311

Query: 308 TDFIRNHKALGVDFASVHIYADTWV-SQTVSDAQLRFTKSWMQAHIEDAEKYL-GMPVIF 367
            DF+RNH+ LGVDFASVH+Y DTW+ +    +AQLRF  SW++AHI DAE  L GMPV+F
Sbjct: 312 ADFVRNHRVLGVDFASVHVYPDTWLPAGATKEAQLRFATSWVEAHIADAEGALGGMPVLF 371

Query: 368 TEFGVSSKD--PGYNLSFRDAVINSVYKTLLDSTKKGGSGGGGLVWQLFPEGTEYMDDGY 427
            EFGVS++     +N + RDA I +VY  +L ST++GG G G L+WQ+FPEGT+YMDDGY
Sbjct: 372 AEFGVSTRGARAAFNATSRDAFIEAVYGAMLRSTRRGGGGAGALLWQVFPEGTDYMDDGY 431

Query: 428 AIVLSKCPSTSNIIALQSTILSILNSICSMTCRWGCKNKN-----------AMDLIFHHE 472
           A+VL +  +T+ I+A  S  L   NS C+ +CRWGC  ++            +D+ FHHE
Sbjct: 432 AVVLPRAAATAGIVAAHSRRLQSFNSRCAWSCRWGCNKRDNDTAETTTAEADVDVSFHHE 491

BLAST of Sed0024783 vs. ExPASy Swiss-Prot
Match: Q0IQJ7 (Mannan endo-1,4-beta-mannosidase 8 OS=Oryza sativa subsp. japonica OX=39947 GN=MAN8 PE=2 SV=2)

HSP 1 Score: 485.0 bits (1247), Expect = 1.0e-135
Identity = 217/364 (59.62%), Postives = 275/364 (75.55%), Query Frame = 0

Query: 73  MVETRQNQFVVEGRPLYVNGFNTYWLMVFAADESTRGKVTEVFKQAASVGLTVCRTWAFN 132
           MVE    Q    GRP  ++GFNTYWLM FAAD++TR +VT    +AA  GL VCRTWAF+
Sbjct: 1   MVECSGTQLWASGRPFIIHGFNTYWLMSFAADQATRPRVTAAIAEAAEAGLNVCRTWAFS 60

Query: 133 DGQWRALQKSPSVYDEQVFKGLDFVISEAKKFKIRVILSLANNWEAYGGKSQYVKWGKAA 192
           DG +RALQ  P  YDE VF+ LDFV+SEAK+  +R+ILSL NNWE YGGK+QYV+WGK A
Sbjct: 61  DGGYRALQTVPFHYDEDVFQALDFVVSEAKRHNMRLILSLCNNWEDYGGKAQYVRWGKEA 120

Query: 193 GLNFTSDDDFFSDPTLRTYYKAHVHTVLNRVNTFTNVTYKEDPTIFAWELMNEPRCPSDP 252
           GL+ TS+DDFFSDPT+++YYKA V  V+ R+NT TN TYK+DPTI AWEL+NEPRCPSDP
Sbjct: 121 GLDLTSEDDFFSDPTIKSYYKAFVEAVVTRINTVTNETYKDDPTILAWELINEPRCPSDP 180

Query: 253 SGDKLQGWIQEMAVYVKSMDPKHLVEVGLEGFYGPSTPGRVQFNPNTYAQQVGTDFIRNH 312
           SGD LQ WI+EMA YVKS+DP HL+E+G+EGFYG STP  +  NP+ Y+   GTDFIRNH
Sbjct: 181 SGDTLQAWIEEMASYVKSIDPVHLLEIGIEGFYGLSTPELLPVNPDEYSGHAGTDFIRNH 240

Query: 313 KALGVDFASVHIYADTWVSQTVSDAQLRFTKSWMQAHIEDAEKYLGMPVIFTEFGVSSKD 372
           +A G+D AS+H+Y+DTW+  ++ +  L+F   WMQ HI DA   LGMP++  EFGVS KD
Sbjct: 241 QAPGIDLASIHVYSDTWLPHSIKENHLQFVDKWMQQHIHDAANLLGMPIVVGEFGVSVKD 300

Query: 373 PGYNLSFRDAVINSVYKTLLDSTKKGGSGGGGLVWQLFPEGTEYMDDGYAIVLSKCPSTS 432
             +   FR+  + +VY+  L S K+G  GGG L+WQLFPEG E+MDDGYA++ +K PST 
Sbjct: 301 GKFGNEFREDFMKTVYRIFLSSWKEGVIGGGCLLWQLFPEGAEHMDDGYAVIFAKSPSTL 360

Query: 433 NIIA 437
           +++A
Sbjct: 361 SLLA 364

BLAST of Sed0024783 vs. ExPASy Swiss-Prot
Match: Q2RBB1 (Mannan endo-1,4-beta-mannosidase 7 OS=Oryza sativa subsp. japonica OX=39947 GN=MAN7 PE=2 SV=3)

HSP 1 Score: 479.6 bits (1233), Expect = 4.2e-134
Identity = 213/366 (58.20%), Postives = 277/366 (75.68%), Query Frame = 0

Query: 71  WSMVETRQNQFVVEGRPLYVNGFNTYWLMVFAADESTRGKVTEVFKQAASVGLTVCRTWA 130
           W+MVE R  Q    GRP  ++GFNTYWLM FAAD++TR +VT    +A   GL VC TWA
Sbjct: 9   WTMVERRGTQLWASGRPFIIHGFNTYWLMSFAADQATRLRVTAAIAEA---GLNVCCTWA 68

Query: 131 FNDGQWRALQKSPSVYDEQVFKGLDFVISEAKKFKIRVILSLANNWEAYGGKSQYVKWGK 190
           F+DG +RALQ +P  YDE VF+ LDFV+SEA++  +R+ILSL NNWE YGGK+QYV+WGK
Sbjct: 69  FSDGGYRALQTAPFHYDEDVFRALDFVVSEARRHNMRLILSLCNNWEDYGGKAQYVRWGK 128

Query: 191 AAGLNFTSDDDFFSDPTLRTYYKAHVHTVLNRVNTFTNVTYKEDPTIFAWELMNEPRCPS 250
            AGL+ TS+DDFFSDPT+++YYKA V  V+ R+NT TN TYK+DPTI AWEL+NEPRCPS
Sbjct: 129 EAGLDLTSEDDFFSDPTIKSYYKAFVEAVVTRINTVTNETYKDDPTILAWELINEPRCPS 188

Query: 251 DPSGDKLQGWIQEMAVYVKSMDPKHLVEVGLEGFYGPSTPGRVQFNPNTYAQQVGTDFIR 310
           DPSGD LQ W++EMA YVKS+DP HL+E+G+EGFYGPS P  +  NP+ Y+   G DFIR
Sbjct: 189 DPSGDTLQAWMEEMASYVKSIDPVHLLEIGIEGFYGPSIPELLPVNPDEYSGHAGIDFIR 248

Query: 311 NHKALGVDFASVHIYADTWVSQTVSDAQLRFTKSWMQAHIEDAEKYLGMPVIFTEFGVSS 370
           NH+A G+D AS+H+Y+D W+ Q++ +  L+F   WMQ HI+DA   LGMP++  EFGVS 
Sbjct: 249 NHQAPGIDLASIHVYSDIWLPQSIKENHLQFVDKWMQQHIDDAANLLGMPIVVGEFGVSV 308

Query: 371 KDPGYNLSFRDAVINSVYKTLLDSTKKGGSGGGGLVWQLFPEGTEYMDDGYAIVLSKCPS 430
           KD  +   FR+  + ++Y+  L S K+G  GGG L+WQLFPEG E+MDDGYA++ +K PS
Sbjct: 309 KDGKFGNEFREDFMKTIYRIFLSSWKEGVIGGGCLLWQLFPEGAEHMDDGYAVIFAKSPS 368

Query: 431 TSNIIA 437
           T +++A
Sbjct: 369 TLSLLA 371

BLAST of Sed0024783 vs. ExPASy Swiss-Prot
Match: Q9FJZ3 (Mannan endo-1,4-beta-mannosidase 7 OS=Arabidopsis thaliana OX=3702 GN=MAN7 PE=2 SV=1)

HSP 1 Score: 416.0 bits (1068), Expect = 5.7e-115
Identity = 192/375 (51.20%), Postives = 253/375 (67.47%), Query Frame = 0

Query: 74  VETRQNQFVVEGRPLYVNGFNTYWLMVFAADESTRGKVTEVFKQAASVGLTVCRTWAFND 133
           V T+  QF + G P Y NGFN YWLM  A+D S R K++  F+ A+  GLTV RTWAF+D
Sbjct: 32  VRTKGVQFSLNGYPYYANGFNAYWLMYVASDPSQRSKISTAFQDASRHGLTVARTWAFSD 91

Query: 134 GQWRALQKSPSVYDEQVFKGLDFVISEAKKFKIRVILSLANNWEAYGGKSQYVKWGKAAG 193
           G +RALQ SP  Y+E +F+GLDF ++EA++  I++ILS ANN+E++GG+ QYV W ++ G
Sbjct: 92  GGYRALQYSPGSYNEDMFQGLDFALAEARRHGIKIILSFANNYESFGGRKQYVDWARSRG 151

Query: 194 LNFTSDDDFFSDPTLRTYYKAHVHTVLNRVNTFTNVTYKEDPTIFAWELMNEPRCPSDPS 253
              +S+DDFF+D  ++ +YK H+  VLNR NTFT V YK+DPTI AWELMNEPRCPSDPS
Sbjct: 152 RPVSSEDDFFTDSLVKDFYKNHIKAVLNRFNTFTKVHYKDDPTIMAWELMNEPRCPSDPS 211

Query: 254 GDKLQGWIQEMAVYVKSMDPKHLVEVGLEGFYGPSTPGRVQFNPNTYAQQVGTDFIRNHK 313
           G  +Q WI EMA +VKS+D  HL+E GLEGFYG S+P     NP     Q GTDFI N++
Sbjct: 212 GRAIQAWITEMAAHVKSLDRNHLLEAGLEGFYGQSSPQSKTLNP---PGQFGTDFIANNR 271

Query: 314 ALGVDFASVHIYADTWVSQTVSDAQLRFTKSWMQAHIEDAEKYLGMPVIFTEFGVSSKDP 373
             G+DF +VH Y D W   +   +Q+ F   W+ AHI+DA+  L  P+I  EFG S K P
Sbjct: 272 IPGIDFVTVHSYPDEWFPDSSEQSQMDFLNKWLDAHIQDAQNVLHKPIILAEFGKSMKKP 331

Query: 374 GYNLSFRDAVINSVYKTLLDSTKKGGSGGGGLVWQLFPEGTEYMDDGYAIVLSKCPSTSN 433
           GY  + RD V N+VY  +  S K+GG+  GGL WQL   G +   DGY I+LS+  ST N
Sbjct: 332 GYTPAQRDIVFNTVYSKIYGSAKRGGAAAGGLFWQLLVNGIDNFQDGYGIILSQSSSTVN 391

Query: 434 IIALQSTILSILNSI 449
           +I+ QS  L+++  I
Sbjct: 392 VISQQSRKLTLIRKI 403

BLAST of Sed0024783 vs. ExPASy TrEMBL
Match: A0A6J1JT09 (Mannan endo-1,4-beta-mannosidase OS=Cucurbita maxima OX=3661 GN=LOC111487238 PE=3 SV=1)

HSP 1 Score: 801.2 bits (2068), Expect = 2.3e-228
Identity = 390/472 (82.63%), Postives = 425/472 (90.04%), Query Frame = 0

Query: 5   NYTMMMMISCSKLIFVVAVFIFSYVEGD----NHHFDFWVEDAYTLNNNFSYSHMSHQGF 64
           +YT++ +    KLIF++AVF+F+ VEGD    N  FD  VED        + +H+SH GF
Sbjct: 4   HYTILSL----KLIFLIAVFVFT-VEGDGVGNNQQFDLLVEDT-------TENHLSH-GF 63

Query: 65  DGVVEKDGDEWSMVETRQNQFVVEGRPLYVNGFNTYWLMVFAADESTRGKVTEVFKQAAS 124
           DGVVEK+ D WSMVETRQNQFVV+G+P YVNGFNTYWLM+FAAD+STRGKVT++FKQAAS
Sbjct: 64  DGVVEKEDDPWSMVETRQNQFVVDGQPFYVNGFNTYWLMIFAADQSTRGKVTDLFKQAAS 123

Query: 125 VGLTVCRTWAFNDGQWRALQKSPSVYDEQVFKGLDFVISEAKKFKIRVILSLANNWEAYG 184
           VGLTVCRTWAFNDGQWRALQKSPSVYDE+VFKGLDFVISEAKK+KIR+ILSLANNWEAYG
Sbjct: 124 VGLTVCRTWAFNDGQWRALQKSPSVYDEEVFKGLDFVISEAKKYKIRLILSLANNWEAYG 183

Query: 185 GKSQYVKWGKAAGLNFTSDDDFFSDPTLRTYYKAHVHTVLNRVNTFTNVTYKEDPTIFAW 244
           GK+QYVKWGKAAGLN TSDDDFFSD T+R+YYKAHV TVLNRVN+FTNV YK+DPTIFAW
Sbjct: 184 GKAQYVKWGKAAGLNLTSDDDFFSDSTVRSYYKAHVKTVLNRVNSFTNVMYKDDPTIFAW 243

Query: 245 ELMNEPRCPSDPSGDKLQGWIQEMAVYVKSMDPKHLVEVGLEGFYGPSTPGRVQFNPNTY 304
           ELMNEPRC SDPSGDKLQGWIQEMAVYVKSMDPKHL+EVGLEGFYGPSTP RVQFNPNTY
Sbjct: 244 ELMNEPRCTSDPSGDKLQGWIQEMAVYVKSMDPKHLLEVGLEGFYGPSTPNRVQFNPNTY 303

Query: 305 AQQVGTDFIRNHKALGVDFASVHIYADTWVSQTVSDAQLRFTKSWMQAHIEDAEKYLGMP 364
           AQQVGTDFIRNHK LGVDFASVHIYADTWVSQT+SDAQL+FTKSWM+AHIEDAEKYLGMP
Sbjct: 304 AQQVGTDFIRNHKVLGVDFASVHIYADTWVSQTISDAQLQFTKSWMEAHIEDAEKYLGMP 363

Query: 365 VIFTEFGVSSKDPGYNLSFRDAVINSVYKTLLDSTKKGGSGGGGLVWQLFPEGTEYMDDG 424
           VIF EFGVSSKDPGYN SFRDA INSVYKTLLDSTKKGGSGGG LVWQLFPEGTE MDDG
Sbjct: 364 VIFAEFGVSSKDPGYNSSFRDAFINSVYKTLLDSTKKGGSGGGSLVWQLFPEGTENMDDG 423

Query: 425 YAIVLSKCPSTSNIIALQSTILSILNSICSMTCRWGCKNKNAMDLIF--HHE 471
           YAIVLSK PSTSN+I LQST LS+ NS+CSM CRWGCK KNAMDL+F  HH+
Sbjct: 424 YAIVLSKSPSTSNVITLQSTRLSLFNSMCSMKCRWGCKKKNAMDLLFLGHHD 462

BLAST of Sed0024783 vs. ExPASy TrEMBL
Match: A0A6J1GQM8 (Mannan endo-1,4-beta-mannosidase OS=Cucurbita moschata OX=3662 GN=LOC111456573 PE=3 SV=1)

HSP 1 Score: 798.1 bits (2060), Expect = 2.0e-227
Identity = 388/472 (82.20%), Postives = 424/472 (89.83%), Query Frame = 0

Query: 5   NYTMMMMISCSKLIFVVAVFIFSYVEGD----NHHFDFWVEDAYTLNNNFSYSHMSHQGF 64
           +YT++ +    KLIF++AVF+F+ VEGD    N  FD  VED        + +H+SH GF
Sbjct: 4   HYTILSL----KLIFLIAVFVFT-VEGDGVGNNQQFDLLVEDT-------TENHLSH-GF 63

Query: 65  DGVVEKDGDEWSMVETRQNQFVVEGRPLYVNGFNTYWLMVFAADESTRGKVTEVFKQAAS 124
           DGVVEK+ D WSMVETRQNQFVV+G+P YVNGFNTYWLM+FAAD+STRGKVTE+FKQAAS
Sbjct: 64  DGVVEKEDDPWSMVETRQNQFVVDGQPFYVNGFNTYWLMIFAADQSTRGKVTELFKQAAS 123

Query: 125 VGLTVCRTWAFNDGQWRALQKSPSVYDEQVFKGLDFVISEAKKFKIRVILSLANNWEAYG 184
           VGLTVCRTWAFNDGQWRALQKSPSVYDE+VFKGLDFVISEAKK+KIR+ILSLANNWEA+G
Sbjct: 124 VGLTVCRTWAFNDGQWRALQKSPSVYDEEVFKGLDFVISEAKKYKIRLILSLANNWEAFG 183

Query: 185 GKSQYVKWGKAAGLNFTSDDDFFSDPTLRTYYKAHVHTVLNRVNTFTNVTYKEDPTIFAW 244
           GK+QYVKWGKAAGLN TSDDDFFSD T+R+YYKAHV TVLNRVN+FTNV YK+DPTIFAW
Sbjct: 184 GKAQYVKWGKAAGLNLTSDDDFFSDSTVRSYYKAHVKTVLNRVNSFTNVMYKDDPTIFAW 243

Query: 245 ELMNEPRCPSDPSGDKLQGWIQEMAVYVKSMDPKHLVEVGLEGFYGPSTPGRVQFNPNTY 304
           ELMNEPRC SDPSGDKLQGWIQEMAVYVKSMDPKHL+EVGLEGFYGPSTP RVQFNPNTY
Sbjct: 244 ELMNEPRCTSDPSGDKLQGWIQEMAVYVKSMDPKHLLEVGLEGFYGPSTPNRVQFNPNTY 303

Query: 305 AQQVGTDFIRNHKALGVDFASVHIYADTWVSQTVSDAQLRFTKSWMQAHIEDAEKYLGMP 364
           AQQVGTDFIRNHK LGVDFASVHIYADTWVSQT+SDA L+FTKSWM+AHIEDAEKYLGMP
Sbjct: 304 AQQVGTDFIRNHKVLGVDFASVHIYADTWVSQTISDAHLQFTKSWMEAHIEDAEKYLGMP 363

Query: 365 VIFTEFGVSSKDPGYNLSFRDAVINSVYKTLLDSTKKGGSGGGGLVWQLFPEGTEYMDDG 424
           V+F EFGVSSKDPGYN SFRDA INSVYKTLLDSTKKGGSGGG LVWQLFPEGTE MDDG
Sbjct: 364 VLFAEFGVSSKDPGYNSSFRDAFINSVYKTLLDSTKKGGSGGGSLVWQLFPEGTENMDDG 423

Query: 425 YAIVLSKCPSTSNIIALQSTILSILNSICSMTCRWGCKNKNAMDLIF--HHE 471
           YAIVLSK PSTSN+I LQST LS+ NS+CSM CRWGCK KNAMDL+F  HH+
Sbjct: 424 YAIVLSKSPSTSNVITLQSTRLSLFNSMCSMKCRWGCKKKNAMDLLFLGHHD 462

BLAST of Sed0024783 vs. ExPASy TrEMBL
Match: A0A6J1FEU9 (Mannan endo-1,4-beta-mannosidase OS=Cucurbita moschata OX=3662 GN=LOC111444855 PE=3 SV=1)

HSP 1 Score: 788.1 bits (2034), Expect = 2.0e-224
Identity = 387/467 (82.87%), Postives = 415/467 (88.87%), Query Frame = 0

Query: 17  LIFVVAVFIF-------SYVE----GDNHHFDFWVEDAYTLNNNFSYSHMSHQGFDGVVE 76
           LI ++AVFIF       S+VE    GD+  FDF VED     N+F+YS MS+ GFDGVV+
Sbjct: 12  LIGLIAVFIFSVEHTVSSFVEGDGDGDDQQFDFSVED--VPENHFTYSDMSN-GFDGVVQ 71

Query: 77  KDGDEWSMVETRQNQFVVEGRPLYVNGFNTYWLMVFAADESTRGKVTEVFKQAASVGLTV 136
           K  D WSMVETRQNQFVV+G+P YVNGFNTYWLMVFAAD+STRGKVTE+FKQAASVGLTV
Sbjct: 72  KKNDAWSMVETRQNQFVVDGQPFYVNGFNTYWLMVFAADQSTRGKVTELFKQAASVGLTV 131

Query: 137 CRTWAFNDGQWRALQKSPSVYDEQVFKGLDFVISEAKKFKIRVILSLANNWEAYGGKSQY 196
           CRTWAFNDGQWRALQKSPSVYDE+VFK LDFVISEAKK+KIR+ILSLANNWEAYGGK+QY
Sbjct: 132 CRTWAFNDGQWRALQKSPSVYDEEVFKALDFVISEAKKYKIRLILSLANNWEAYGGKAQY 191

Query: 197 VKWGKAAGLNFTSDDDFFSDPTLRTYYKAHVHTVLNRVNTFTNVTYKEDPTIFAWELMNE 256
           VKWG+AAGLN TSDDDFFSDPTLR+YYKAHV TVLNRVNT+TNV YKEDPTIFAWELMNE
Sbjct: 192 VKWGRAAGLNLTSDDDFFSDPTLRSYYKAHVATVLNRVNTYTNVMYKEDPTIFAWELMNE 251

Query: 257 PRCPSDPSGDKLQGWIQEMAVYVKSMDPKHLVEVGLEGFYGPSTPGRVQFNPNTYAQQVG 316
           PRC SDPSGDKLQGWIQEMAV+VKSMDPKHLVEVGLEGFYGPSTP RVQ NPNTYAQQVG
Sbjct: 252 PRCTSDPSGDKLQGWIQEMAVFVKSMDPKHLVEVGLEGFYGPSTPNRVQLNPNTYAQQVG 311

Query: 317 TDFIRNHKALGVDFASVHIYADTWVSQTVSDAQLRFTKSWMQAHIEDAEKYLGMPVIFTE 376
           TDFIRNHK LGVDFASVHIYADTWVS +VSD  L+FTKSWM AHIEDAEKYLGMPVIF E
Sbjct: 312 TDFIRNHKVLGVDFASVHIYADTWVSPSVSDTHLQFTKSWMDAHIEDAEKYLGMPVIFAE 371

Query: 377 FGVSSKDPGYNLSFRDAVINSVYKTLLDSTKKGGSGGGGLVWQLFPEGTEYMDDGYAIVL 436
           FGVSSKDPGYN +FRDAV+N VYKTLL STKKGGSGGG LVWQLFP+GT+YMDDGYAIVL
Sbjct: 372 FGVSSKDPGYNSTFRDAVLNLVYKTLLGSTKKGGSGGGSLVWQLFPDGTDYMDDGYAIVL 431

Query: 437 SKCPSTSNIIALQSTILSILNSICSMTCRWGCKNKNAMDLIF--HHE 471
           SK PSTSNII+L ST LSI NSICSM CRWGCK KN MDLIF  HH+
Sbjct: 432 SKSPSTSNIISLHSTRLSIFNSICSMRCRWGCKKKNLMDLIFPDHHD 475

BLAST of Sed0024783 vs. ExPASy TrEMBL
Match: A0A0A0KJ68 (Mannan endo-1,4-beta-mannosidase OS=Cucumis sativus OX=3659 GN=Csa_6G405340 PE=3 SV=1)

HSP 1 Score: 788.1 bits (2034), Expect = 2.0e-224
Identity = 381/467 (81.58%), Postives = 418/467 (89.51%), Query Frame = 0

Query: 16  KLIFVVAVF-------IFSYVE--GDNHHFDFWVEDAYTLNNNFSYSHMSHQGFDGVVEK 75
           KL+F++ V        I S+VE  GD+  FDF VEDA    N+F+YS MSH GFD VV+K
Sbjct: 11  KLVFLIVVLISCVEYTISSFVEGNGDDQPFDFSVEDA--TENHFTYSDMSH-GFDRVVQK 70

Query: 76  DGDEWSMVETRQNQFVVEGRPLYVNGFNTYWLMVFAADESTRGKVTEVFKQAASVGLTVC 135
           + D WSMVET+QNQFVV+G+P YVNGFNTYWLM+FAAD+STRGKVTEVFKQAASVGLTVC
Sbjct: 71  ESDAWSMVETKQNQFVVDGQPFYVNGFNTYWLMIFAADQSTRGKVTEVFKQAASVGLTVC 130

Query: 136 RTWAFNDGQWRALQKSPSVYDEQVFKGLDFVISEAKKFKIRVILSLANNWEAYGGKSQYV 195
           RTWAFNDGQWRALQKSPSVYDE+VFKGLDFVISEAKKFKIR+ILSLANNWEA+GGK+QYV
Sbjct: 131 RTWAFNDGQWRALQKSPSVYDEEVFKGLDFVISEAKKFKIRLILSLANNWEAFGGKAQYV 190

Query: 196 KWGKAAGLNFTSDDDFFSDPTLRTYYKAHVHTVLNRVNTFTNVTYKEDPTIFAWELMNEP 255
           KWGKAAGLN TSDDDFF+DPTLR+YYKAHV TVLNRVNT+TNVTYKEDPTIFAWELMNEP
Sbjct: 191 KWGKAAGLNLTSDDDFFTDPTLRSYYKAHVKTVLNRVNTYTNVTYKEDPTIFAWELMNEP 250

Query: 256 RCPSDPSGDKLQGWIQEMAVYVKSMDPKHLVEVGLEGFYGPSTPGRVQFNPNTYAQQVGT 315
           RC SDPSG+ LQGWIQEMAV+VKSMDPKHL+EVGLEGFYGPSTP RVQFNPNTYAQQVGT
Sbjct: 251 RCTSDPSGNTLQGWIQEMAVFVKSMDPKHLLEVGLEGFYGPSTPNRVQFNPNTYAQQVGT 310

Query: 316 DFIRNHKALGVDFASVHIYADTWVSQTVSDAQLRFTKSWMQAHIEDAEKYLGMPVIFTEF 375
           DFIRNHK LGVDFASVHIYADTWVSQ +SDA L+FTKSWM+AHIEDAEKYLGMPVIF EF
Sbjct: 311 DFIRNHKVLGVDFASVHIYADTWVSQAISDAHLQFTKSWMEAHIEDAEKYLGMPVIFAEF 370

Query: 376 GVSSKDPGYNLSFRDAVINSVYKTLLDSTKKGGSGGGGLVWQLFPEGTEYMDDGYAIVLS 435
           GVS+KDPGYN ++RD  ++SVYKTLLDSTKKGGSGGG LVWQLFPEGT+YMDDGYAIVLS
Sbjct: 371 GVSTKDPGYNSTYRDKYLSSVYKTLLDSTKKGGSGGGSLVWQLFPEGTDYMDDGYAIVLS 430

Query: 436 KCPSTSNIIALQSTILSILNSICSMTCRWGCKNKNAMDLIF-HHEIH 473
             PSTSNII+L ST +SI NSICSM CRWGCK KN MD+IF +H  H
Sbjct: 431 NSPSTSNIISLHSTRMSIFNSICSMKCRWGCKKKNVMDIIFLNHNDH 474

BLAST of Sed0024783 vs. ExPASy TrEMBL
Match: A0A5A7SYV0 (Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold147G00330 PE=3 SV=1)

HSP 1 Score: 787.7 bits (2033), Expect = 2.7e-224
Identity = 379/464 (81.68%), Postives = 416/464 (89.66%), Query Frame = 0

Query: 16  KLIFVVAVF-------IFSYVE--GDNHHFDFWVEDAYTLNNNFSYSHMSHQGFDGVVEK 75
           KL+F++ VF       I S+VE  GD+  FDF VEDA    N+F+YS MSH GF+GV + 
Sbjct: 11  KLVFLIVVFISCVEYTISSFVEGNGDDQPFDFSVEDA--TENHFTYSDMSH-GFEGVDQN 70

Query: 76  DGDEWSMVETRQNQFVVEGRPLYVNGFNTYWLMVFAADESTRGKVTEVFKQAASVGLTVC 135
           D D WSMVET+QNQFVV+G+P YVNGFNTYWLM+FAAD+STRGKVTEVFKQAASVGLTVC
Sbjct: 71  DNDAWSMVETKQNQFVVDGQPFYVNGFNTYWLMIFAADQSTRGKVTEVFKQAASVGLTVC 130

Query: 136 RTWAFNDGQWRALQKSPSVYDEQVFKGLDFVISEAKKFKIRVILSLANNWEAYGGKSQYV 195
           RTWAFNDGQWRALQKSPSVYDE+VFKGLDFVISEAKK+KIR+ILSLANNWEA+GGK+QYV
Sbjct: 131 RTWAFNDGQWRALQKSPSVYDEEVFKGLDFVISEAKKYKIRLILSLANNWEAFGGKAQYV 190

Query: 196 KWGKAAGLNFTSDDDFFSDPTLRTYYKAHVHTVLNRVNTFTNVTYKEDPTIFAWELMNEP 255
           KWGKAAGLN TSDDDFF+DPTLR+YYKAHV TVLNRVNT+TNVTYKEDPTIFAWELMNEP
Sbjct: 191 KWGKAAGLNLTSDDDFFTDPTLRSYYKAHVKTVLNRVNTYTNVTYKEDPTIFAWELMNEP 250

Query: 256 RCPSDPSGDKLQGWIQEMAVYVKSMDPKHLVEVGLEGFYGPSTPGRVQFNPNTYAQQVGT 315
           RC SDPSG+ LQGWIQEMAV+VKSMDPKHL+EVGLEGFYGPSTP RVQFNPNTYAQQVGT
Sbjct: 251 RCTSDPSGNTLQGWIQEMAVFVKSMDPKHLLEVGLEGFYGPSTPNRVQFNPNTYAQQVGT 310

Query: 316 DFIRNHKALGVDFASVHIYADTWVSQTVSDAQLRFTKSWMQAHIEDAEKYLGMPVIFTEF 375
           DFIRNHK LGVDFASVHIYADTWVSQ +SDA L+FTKSWM+AHIEDAEKYLGMPVIF EF
Sbjct: 311 DFIRNHKVLGVDFASVHIYADTWVSQAISDAHLQFTKSWMEAHIEDAEKYLGMPVIFAEF 370

Query: 376 GVSSKDPGYNLSFRDAVINSVYKTLLDSTKKGGSGGGGLVWQLFPEGTEYMDDGYAIVLS 435
           GVSSKDPGYN ++RD  ++SVYK LLDSTKKGGSGGG LVWQLFPEGT+YMDDGYAIVLS
Sbjct: 371 GVSSKDPGYNSTYRDKYLSSVYKMLLDSTKKGGSGGGSLVWQLFPEGTDYMDDGYAIVLS 430

Query: 436 KCPSTSNIIALQSTILSILNSICSMTCRWGCKNKNAMDLIFHHE 471
           K PSTSNII+L ST +SI NSICSM CRWGCK KN MD IF ++
Sbjct: 431 KSPSTSNIISLHSTRMSIFNSICSMKCRWGCKKKNVMDFIFPNQ 471

BLAST of Sed0024783 vs. TAIR 10
Match: AT5G01930.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 643.7 bits (1659), Expect = 1.2e-184
Identity = 292/428 (68.22%), Postives = 356/428 (83.18%), Query Frame = 0

Query: 46  LNNNFSYSHMSHQGFDGVVEKDGDE-WSMVETRQNQFVVEGRPLYVNGFNTYWLMVFAAD 105
           L  N + + +  +  +   E  G+E W MV+ +  QF + G+P YVNGFNTYW+M  AAD
Sbjct: 19  LTQNRALADLDSESHEVNSESVGEEQWEMVQRKGMQFTLNGQPFYVNGFNTYWMMTLAAD 78

Query: 106 ESTRGKVTEVFKQAASVGLTVCRTWAFNDGQWRALQKSPSVYDEQVFKGLDFVISEAKKF 165
            STRGKVTEVF+QA++VG+TV RTWAFNDGQWRALQKSPSVYDE+VFK LDFV+SEA+K+
Sbjct: 79  NSTRGKVTEVFQQASAVGMTVGRTWAFNDGQWRALQKSPSVYDEEVFKALDFVLSEARKY 138

Query: 166 KIRVILSLANNWEAYGGKSQYVKWGKAAGLNFTSDDDFFSDPTLRTYYKAHVHTVLNRVN 225
           KIR+ILSL NNW+AYGGK+QYVKWG A+GLN TSDDDFF++PTLR +Y++HV TVLNRVN
Sbjct: 139 KIRLILSLVNNWDAYGGKAQYVKWGNASGLNLTSDDDFFTNPTLRNFYQSHVRTVLNRVN 198

Query: 226 TFTNVTYKEDPTIFAWELMNEPRCPSDPSGDKLQGWIQEMAVYVKSMDPKHLVEVGLEGF 285
           TFTN+TYK DPTIFAWELMNEPRCPSDPSGDKLQ WIQEMAV+VKS+D KHLVE+GLEGF
Sbjct: 199 TFTNITYKNDPTIFAWELMNEPRCPSDPSGDKLQSWIQEMAVFVKSLDAKHLVEIGLEGF 258

Query: 286 YGPSTPGRVQFNPNTYAQQVGTDFIRNHKALGVDFASVHIYADTWVSQTVSDAQLRFTKS 345
           YGPS P R +FNPN YA QVGTDFIRN++ LG+DFASVH+Y D+W+S  VS++ L FT S
Sbjct: 259 YGPSAPARTRFNPNPYAAQVGTDFIRNNQVLGIDFASVHVYPDSWISPAVSNSFLEFTSS 318

Query: 346 WMQAHIEDAEKYLGMPVIFTEFGVSSKDPGYNLSFRDAVINSVYKTLLDSTKKGGSGGGG 405
           WMQAH+EDAE YLGMPV+FTEFGVS+ DPG+N SFRD ++N+VYK  L+ST+KGG+G G 
Sbjct: 319 WMQAHVEDAEMYLGMPVLFTEFGVSAHDPGFNTSFRDMMLNTVYKMTLNSTRKGGAGAGS 378

Query: 406 LVWQLFPEGTEYMDDGYAIVLSKCPSTSNIIALQSTILSILNSICSMTCRWGCKNKN--A 465
           LVWQ+FP+G E+MDDGYA+ L++  + S II+LQS  L+I NS+CS  CRWGCK KN  A
Sbjct: 379 LVWQVFPQGAEFMDDGYAVYLTRAHTASKIISLQSKRLAIFNSLCSWRCRWGCKKKNQTA 438

Query: 466 MDLIFHHE 471
           +D +  H+
Sbjct: 439 LDALLSHD 446

BLAST of Sed0024783 vs. TAIR 10
Match: AT5G66460.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 416.0 bits (1068), Expect = 4.0e-116
Identity = 192/375 (51.20%), Postives = 253/375 (67.47%), Query Frame = 0

Query: 74  VETRQNQFVVEGRPLYVNGFNTYWLMVFAADESTRGKVTEVFKQAASVGLTVCRTWAFND 133
           V T+  QF + G P Y NGFN YWLM  A+D S R K++  F+ A+  GLTV RTWAF+D
Sbjct: 32  VRTKGVQFSLNGYPYYANGFNAYWLMYVASDPSQRSKISTAFQDASRHGLTVARTWAFSD 91

Query: 134 GQWRALQKSPSVYDEQVFKGLDFVISEAKKFKIRVILSLANNWEAYGGKSQYVKWGKAAG 193
           G +RALQ SP  Y+E +F+GLDF ++EA++  I++ILS ANN+E++GG+ QYV W ++ G
Sbjct: 92  GGYRALQYSPGSYNEDMFQGLDFALAEARRHGIKIILSFANNYESFGGRKQYVDWARSRG 151

Query: 194 LNFTSDDDFFSDPTLRTYYKAHVHTVLNRVNTFTNVTYKEDPTIFAWELMNEPRCPSDPS 253
              +S+DDFF+D  ++ +YK H+  VLNR NTFT V YK+DPTI AWELMNEPRCPSDPS
Sbjct: 152 RPVSSEDDFFTDSLVKDFYKNHIKAVLNRFNTFTKVHYKDDPTIMAWELMNEPRCPSDPS 211

Query: 254 GDKLQGWIQEMAVYVKSMDPKHLVEVGLEGFYGPSTPGRVQFNPNTYAQQVGTDFIRNHK 313
           G  +Q WI EMA +VKS+D  HL+E GLEGFYG S+P     NP     Q GTDFI N++
Sbjct: 212 GRAIQAWITEMAAHVKSLDRNHLLEAGLEGFYGQSSPQSKTLNP---PGQFGTDFIANNR 271

Query: 314 ALGVDFASVHIYADTWVSQTVSDAQLRFTKSWMQAHIEDAEKYLGMPVIFTEFGVSSKDP 373
             G+DF +VH Y D W   +   +Q+ F   W+ AHI+DA+  L  P+I  EFG S K P
Sbjct: 272 IPGIDFVTVHSYPDEWFPDSSEQSQMDFLNKWLDAHIQDAQNVLHKPIILAEFGKSMKKP 331

Query: 374 GYNLSFRDAVINSVYKTLLDSTKKGGSGGGGLVWQLFPEGTEYMDDGYAIVLSKCPSTSN 433
           GY  + RD V N+VY  +  S K+GG+  GGL WQL   G +   DGY I+LS+  ST N
Sbjct: 332 GYTPAQRDIVFNTVYSKIYGSAKRGGAAAGGLFWQLLVNGIDNFQDGYGIILSQSSSTVN 391

Query: 434 IIALQSTILSILNSI 449
           +I+ QS  L+++  I
Sbjct: 392 VISQQSRKLTLIRKI 403

BLAST of Sed0024783 vs. TAIR 10
Match: AT3G10890.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 389.0 bits (998), Expect = 5.3e-108
Identity = 181/381 (47.51%), Postives = 245/381 (64.30%), Query Frame = 0

Query: 66  KDGDEWSMVETRQNQFVVEGRPLYVNGFNTYWLMVFAADESTRGKVTEVFKQAASVGLTV 125
           K       V  +  QF++ G+P Y NGFN YWL   A D +TR K+T VF+ A    LT+
Sbjct: 24  KSASSDGFVSRKGVQFILNGKPFYANGFNAYWLAYEATDSTTRFKITYVFQNATIHDLTI 83

Query: 126 CRTWAFNDGQWRALQKSPSVYDEQVFKGLDFVISEAKKFKIRVILSLANNWEAYGGKSQY 185
            RTW F DG +RALQ +P VYDE+ F+GLDF I+EAK+  I++I++  NN+  +GG+ QY
Sbjct: 84  VRTWGFRDGGYRALQIAPGVYDEKTFQGLDFAIAEAKRLGIKMIITFVNNYSDFGGRKQY 143

Query: 186 VKWGKAAGLNFTSDDDFFSDPTLRTYYKAHVHTVLNRVNTFTNVTYKEDPTIFAWELMNE 245
           V W K  G N +SDDDF+++P ++ YYK HV T++NRVNTFT V YK++PTI  WELMNE
Sbjct: 144 VDWAKNTGQNVSSDDDFYTNPLVKQYYKNHVKTMVNRVNTFTKVEYKDEPTIMGWELMNE 203

Query: 246 PRCPSDPSGDKLQGWIQEMAVYVKSMDPKHLVEVGLEGFYGPSTPGR-VQFNPNTYAQQV 305
           P+C +DPSG  L  W+ EMA+YVKS+D KHL+  GLEGFYG S+P R    NP   A  +
Sbjct: 204 PQCRADPSGKTLTAWMNEMALYVKSVDSKHLLSTGLEGFYGDSSPQRKTSLNP-VAANVL 263

Query: 306 GTDFIRNHKALGVDFASVHIYADTWVSQTVSDAQLRFTKSWMQAHIEDAEKYLGMPVIFT 365
           GTDFI NHK   +DFAS+H Y D W       ++L   + W++ H+EDA+  L  P+I  
Sbjct: 264 GTDFIANHKLDAIDFASIHSYPDLWFPNLDEKSRLNLLRKWLECHLEDAQNILKKPLILG 323

Query: 366 EFGVSSKDPGYNLSFRDAVINSVYKTLLDSTKKGGSGGGGLVWQLFPEGTEYMDDGYAIV 425
           EFG  +  PGY  + RDAV N+ + T+ +S +KGG   G L W +  +G     D  +IV
Sbjct: 324 EFGKPTNTPGYTQAQRDAVFNATFDTIYESAEKGGPAAGALFWHVISDGMNNFKDPLSIV 383

Query: 426 LSKCPSTSNIIALQSTILSIL 446
           LS+  +T NII  +S  L ++
Sbjct: 384 LSENSTTVNIITEESRKLGLI 403

BLAST of Sed0024783 vs. TAIR 10
Match: AT4G28320.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 388.7 bits (997), Expect = 6.9e-108
Identity = 183/367 (49.86%), Postives = 248/367 (67.57%), Query Frame = 0

Query: 74  VETRQNQFVVEGRPLYVNGFNTYWLMVFAADESTRGKVTEVFKQAASVGLTVCRTWAFND 133
           V+    QFVV+ +PLYVNG+N+YW M  A DE +R  V E+ +  A +GLTVCRTWAFND
Sbjct: 42  VKRNGTQFVVDDKPLYVNGWNSYWFMDHAVDEHSRNLVGEMLEAGAKMGLTVCRTWAFND 101

Query: 134 GQWRALQKSPSVYDEQVFKGLDFVISEAKKFKIRVILSLANNWEAYGGKSQYVKWGKAAG 193
           G + ALQ SP  +DE+VF+ LD VI+EA+K  +R++LSL NN +AYGGK+QYVKW    G
Sbjct: 102 GGYNALQISPGRFDERVFQALDHVIAEARKHDVRLLLSLVNNLQAYGGKTQYVKWAWQEG 161

Query: 194 LNF-TSDDDFFSDPTLRTYYKAHVHTVLNRVNTFTNVTYKEDPTIFAWELMNEPRCPSDP 253
           +   +S+D FF DP++R Y+K ++  +L R N+ T + Y+ DPTIFAWEL+NEPRC +D 
Sbjct: 162 VGLSSSNDSFFFDPSIRNYFKNYLKVLLTRKNSVTGIEYRNDPTIFAWELINEPRCTTDV 221

Query: 254 SGDKLQGWIQEMAVYVKSMDPKHLVEVGLEGFYGPSTPGRVQFNPNTYAQQVGTDFIRNH 313
           SG  LQ WI EM  ++KS+D KHL+ VGLEGFYGP++P  +  NP  +A Q+GTDF++N 
Sbjct: 222 SGKTLQDWIDEMTGFIKSIDDKHLLTVGLEGFYGPNSPKGLTVNPEQWASQLGTDFVQNS 281

Query: 314 KALGVDFASVHIYADTWVSQTVSDAQLRFTKSWMQAHIEDAEKYLGMPVIFTEFGVSSKD 373
            +  +DFASVHIY D W      + +L+F   WMQ+HIED  K L  PV+FTEFG+S+++
Sbjct: 282 NSSNIDFASVHIYPDHWFHNQTFEEKLKFVVKWMQSHIEDGLKELKKPVLFTEFGLSNQN 341

Query: 374 PGYNLSFRDAVINSVYKTLLDSTKKGGSGGGGLVWQLFPEGTEYMDDGYAIVLSKCPSTS 433
             Y  S RD     ++  +  S K+  SG G LVWQLF EG E  +D + IV  +  S  
Sbjct: 342 KDYEPSQRDKFYRIIFDVVYKSAKRKKSGAGTLVWQLFMEGMETFNDDFGIVPHEQDSIY 401

Query: 434 NIIALQS 440
            ++  QS
Sbjct: 402 KLMIEQS 408

BLAST of Sed0024783 vs. TAIR 10
Match: AT2G20680.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 384.8 bits (987), Expect = 1.0e-106
Identity = 183/379 (48.28%), Postives = 253/379 (66.75%), Query Frame = 0

Query: 66  KDGDEWSMVETRQNQFVVEGRPLYVNGFNTYWLMVFAADESTRGKVTEVFKQAASVGLTV 125
           K   E + V+    QFVV+G+ LYVNG+N+YW M  A ++ +R +V+ + +  A +GLTV
Sbjct: 35  KTEGELAFVKRNGTQFVVDGKALYVNGWNSYWFMDHAVNDHSRHRVSAMLEAGAKMGLTV 94

Query: 126 CRTWAFNDGQWRALQKSPSVYDEQVFKGLDFVISEAKKFKIRVILSLANNWEAYGGKSQY 185
           CRTWAFNDG + ALQ SP  +DE+VFK LD VI+EAK   +R++LSL NN +AYGGK+QY
Sbjct: 95  CRTWAFNDGGYNALQISPGRFDERVFKALDHVIAEAKTHGVRLLLSLVNNLQAYGGKTQY 154

Query: 186 VKWGKAAGLNF-TSDDDFFSDPTLRTYYKAHVHTVLNRVNTFTNVTYKEDPTIFAWELMN 245
           V W    G+   +S+D FF DP++R Y+K ++  +L R N+ T + Y+ DPTIFAWEL+N
Sbjct: 155 VNWAWQEGVGLSSSNDSFFFDPSIRRYFKNYLTVLLTRKNSLTGIEYRNDPTIFAWELIN 214

Query: 246 EPRCPSDPSGDKLQGWIQEMAVYVKSMDPKHLVEVGLEGFYGPSTPGRVQFNPNTYAQQV 305
           EPRC SD SGD LQ WI EM  ++KS+D KHL+ VGLEGFYGPS+P ++  NP  +A ++
Sbjct: 215 EPRCMSDVSGDTLQDWINEMTAFIKSIDNKHLLTVGLEGFYGPSSPKKLTVNPERWASEL 274

Query: 306 GTDFIRNHKALGVDFASVHIYADTWVSQTVSDAQLRFTKSWMQAHIEDAEKYLGMPVIFT 365
           G+DF+RN  +  +DFASVHIY D W      + +L+F   WM +HIED +K L  PV+FT
Sbjct: 275 GSDFVRNSDSPNIDFASVHIYPDHWFHDQGFEEKLKFVVKWMLSHIEDGDKELKKPVLFT 334

Query: 366 EFGVSSKDPGYNLSFRDAVINSVYKTLLDSTKKGGSGGGGLVWQLFPEGTEYMDDGYAIV 425
           EFG+S+ +  Y+ S RD    +++  +  S K+  SG G LVWQ   EG E  +D + IV
Sbjct: 335 EFGLSNLNKDYDPSQRDRFYRTIFDVIYKSAKRKRSGAGTLVWQFLIEGMEGFNDDFGIV 394

Query: 426 LSKCPSTSNIIALQSTILS 444
             +  S   ++  QS  LS
Sbjct: 395 PWEQDSIQRLMIEQSCRLS 413

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038886206.13.7e-22882.83mannan endo-1,4-beta-mannosidase 6 isoform X1 [Benincasa hispida][more]
XP_023540740.14.8e-22882.63mannan endo-1,4-beta-mannosidase 6-like [Cucurbita pepo subsp. pepo][more]
XP_022990348.14.8e-22882.63mannan endo-1,4-beta-mannosidase 6-like [Cucurbita maxima][more]
XP_022954273.14.1e-22782.20mannan endo-1,4-beta-mannosidase 6-like [Cucurbita moschata] >KAG6602316.1 Manna... [more]
XP_022938707.14.2e-22482.87mannan endo-1,4-beta-mannosidase 6-like [Cucurbita moschata] >XP_022938708.1 man... [more]
Match NameE-valueIdentityDescription
Q9LZV31.7e-18368.22Mannan endo-1,4-beta-mannosidase 6 OS=Arabidopsis thaliana OX=3702 GN=MAN6 PE=2 ... [more]
Q5W6G04.6e-14960.57Putative mannan endo-1,4-beta-mannosidase 5 OS=Oryza sativa subsp. japonica OX=3... [more]
Q0IQJ71.0e-13559.62Mannan endo-1,4-beta-mannosidase 8 OS=Oryza sativa subsp. japonica OX=39947 GN=M... [more]
Q2RBB14.2e-13458.20Mannan endo-1,4-beta-mannosidase 7 OS=Oryza sativa subsp. japonica OX=39947 GN=M... [more]
Q9FJZ35.7e-11551.20Mannan endo-1,4-beta-mannosidase 7 OS=Arabidopsis thaliana OX=3702 GN=MAN7 PE=2 ... [more]
Match NameE-valueIdentityDescription
A0A6J1JT092.3e-22882.63Mannan endo-1,4-beta-mannosidase OS=Cucurbita maxima OX=3661 GN=LOC111487238 PE=... [more]
A0A6J1GQM82.0e-22782.20Mannan endo-1,4-beta-mannosidase OS=Cucurbita moschata OX=3662 GN=LOC111456573 P... [more]
A0A6J1FEU92.0e-22482.87Mannan endo-1,4-beta-mannosidase OS=Cucurbita moschata OX=3662 GN=LOC111444855 P... [more]
A0A0A0KJ682.0e-22481.58Mannan endo-1,4-beta-mannosidase OS=Cucumis sativus OX=3659 GN=Csa_6G405340 PE=3... [more]
A0A5A7SYV02.7e-22481.68Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27... [more]
Match NameE-valueIdentityDescription
AT5G01930.11.2e-18468.22Glycosyl hydrolase superfamily protein [more]
AT5G66460.14.0e-11651.20Glycosyl hydrolase superfamily protein [more]
AT3G10890.15.3e-10847.51Glycosyl hydrolase superfamily protein [more]
AT4G28320.16.9e-10849.86Glycosyl hydrolase superfamily protein [more]
AT2G20680.11.0e-10648.28Glycosyl hydrolase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Chayote (edule) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.20.20.80Glycosidasescoord: 71..446
e-value: 2.2E-130
score: 436.9
NoneNo IPR availablePANTHERPTHR31451:SF54MANNAN ENDO-1,4-BETA-MANNOSIDASE 6coord: 11..466
IPR001547Glycoside hydrolase, family 5PFAMPF00150Cellulasecoord: 85..373
e-value: 2.3E-16
score: 60.0
IPR045053Mannan endo-1,4-beta-mannosidase-likePANTHERPTHR31451FAMILY NOT NAMEDcoord: 11..466
IPR017853Glycoside hydrolase superfamilySUPERFAMILY51445(Trans)glycosidasescoord: 73..429

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sed0024783.1Sed0024783.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006352 DNA-templated transcription, initiation
biological_process GO:0009845 seed germination
biological_process GO:0006413 translational initiation
biological_process GO:0071704 organic substance metabolic process
cellular_component GO:0005576 extracellular region
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005634 nucleus
cellular_component GO:0030880 RNA polymerase complex
molecular_function GO:0016985 mannan endo-1,4-beta-mannosidase activity
molecular_function GO:0000166 nucleotide binding
molecular_function GO:0003743 translation initiation factor activity
molecular_function GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds