Homology
BLAST of Sed0024525 vs. NCBI nr
Match:
XP_038905686.1 (subtilisin-like protease SBT5.3 [Benincasa hispida])
HSP 1 Score: 1033.1 bits (2670), Expect = 1.2e-297
Identity = 507/736 (68.89%), Postives = 605/736 (82.20%), Query Frame = 0
Query: 6 SYIVYLGGQKFGKKSSIFDVKLAKQSQFDILESVLESKVAVKESLIYSYSKYMNGFAALL 65
SYIVYLG FG S +DV+ A +SQ+DIL SV SK+A KES++YSYS+Y+NGFAA+L
Sbjct: 27 SYIVYLGLHSFGLNPSKYDVQRATESQYDILSSVTGSKLAAKESIMYSYSRYINGFAAVL 86
Query: 66 DEKQVVDLEKNPSVVSVFENTIRHLHTTRSWHFLGMENDAGIPANSIWNAAKFGEDVIIG 125
DEK+ + L KNPSVVSVFEN R LHTT+SW FLG+++DAGIP+NSIW AA+FGED IIG
Sbjct: 87 DEKEAMALAKNPSVVSVFENKERKLHTTKSWSFLGVDSDAGIPSNSIWKAARFGEDTIIG 146
Query: 126 NLDTGVWPESKSFDDTGYGPVPKRWMGECDGGANFTCNRKLIGARYFYQGIESTNGPLNI 185
NLDTG WPESKSF+D GYGPVP RW G CDGGANF CNRKLIGARYF QG NGPLN+
Sbjct: 147 NLDTGAWPESKSFNDAGYGPVPSRWRGACDGGANFRCNRKLIGARYFNQGFAMANGPLNV 206
Query: 186 SMHTARDHEGHGSHTLSTAGGNLVPGANVFGSGNGTAKGGSPKARVVAYKVCWP---GGC 245
S +TARD EGHGSHTLSTAGGN VPGAN+FG GNGTAKGGSPKARV AYKVCWP GGC
Sbjct: 207 SFNTARDKEGHGSHTLSTAGGNFVPGANIFGYGNGTAKGGSPKARVAAYKVCWPAPTGGC 266
Query: 246 SDADILAGFEAAMDDGVDVLSVSLGSRATQFATDPVSVASFHAVQQGIVVVCSAGNNGPD 305
D+DILAGFEAA+ DGVDVLSVSLG+ A +FA D VS+ +FHAVQ+GIVVVCS GN+GP
Sbjct: 267 FDSDILAGFEAAIGDGVDVLSVSLGTGAQEFAYDAVSIGAFHAVQKGIVVVCSGGNDGPS 326
Query: 306 PATVANIAPWMFTVAASTIDREFVSYVGLGNKKQIKGSSLSSEGLAVGSLHPLINAVDAK 365
P TV+N++PWMFTVAASTIDR+F SYV LGNKK I+GSSLSS GL +PLINA++AK
Sbjct: 327 PGTVSNVSPWMFTVAASTIDRDFASYVLLGNKKHIRGSSLSSSGLRGRKFYPLINAIEAK 386
Query: 366 AANVPDKLAQLCEKDSLDPAKAKGKIVVCLCGDDENVDQGFEVRRAGGVGLILVNDEEDG 425
AAN D LAQ CE+ SLDPAKAKGKI+VCL G++ V++ F V AGGVG+I+VND++DG
Sbjct: 387 AANATDSLAQFCEQGSLDPAKAKGKIIVCLRGENARVEKSFVVLHAGGVGMIMVNDQKDG 446
Query: 426 ADTISDPHFLPASHVSHEDGLLIAQYMKSTTTPEASISAVKTEIGVKPSPVMAVFSSRGP 485
+ T++D H LPA+HVS+ DGL I+QY+KST TP A I+ VKTE+G+KPSPVMA FSSRGP
Sbjct: 447 SGTLADAHILPATHVSYTDGLSISQYIKSTKTPVAYITHVKTEVGIKPSPVMADFSSRGP 506
Query: 486 NPIEESMIKPDITAPGVTIIASMPEAVAASKSALDTRRVPFNILSGTSMACPHISGIAGL 545
N I E+M+KPDITAPGV IIAS+ A+ S DTRRVPFN+ SGTSM+CPHISG+AGL
Sbjct: 507 NSITEAMLKPDITAPGVNIIASVTIDATATDSPFDTRRVPFNVESGTSMSCPHISGVAGL 566
Query: 546 LRVLNPTWSPAAIRSAMMTTAITIDNTNHSMLNSNKADATPFDYGAGYVQPNNAMDPGLI 605
L+ L PTWSPAAI+SA+MTTA T DNT ++ ++ K ATPFDYGAG+V PN+AMDPGL+
Sbjct: 567 LKTLYPTWSPAAIKSAIMTTAKTRDNTQRTISDTAKMKATPFDYGAGHVHPNSAMDPGLV 626
Query: 606 YNTTIEDYLNFLCAKKYTDAELKKFSTQAFLCEESFAVTDLNYPSISIPKLAAGAPVTIN 665
Y+TTI+DYLNFLCA+ Y LKKF + F+C +SFA+TDLNYPSIS+P+L GAPVT+N
Sbjct: 627 YDTTIDDYLNFLCARGYNSVALKKFYNKPFICAKSFAITDLNYPSISVPELRIGAPVTVN 686
Query: 666 RRLLNVGSPGTYVAQVKVPESVQAKVEPSSLQFSKVDEEKSFKIVFQHVG-ERRVSYEFG 725
RR+ NVG+PGTYVA+VK ++ VEPS+LQF+ V EEK+FK+VFQ+ G E+R + FG
Sbjct: 687 RRVKNVGTPGTYVARVKASPAISVSVEPSTLQFNSVGEEKAFKVVFQYKGKEQRQGHVFG 746
Query: 726 TLEWSDGKHFVRSPLA 738
TL WSDGKHFVRSP+A
Sbjct: 747 TLIWSDGKHFVRSPIA 762
BLAST of Sed0024525 vs. NCBI nr
Match:
XP_022949775.1 (subtilisin-like protease SBT5.3 [Cucurbita moschata])
HSP 1 Score: 1018.5 bits (2632), Expect = 3.0e-293
Identity = 504/741 (68.02%), Postives = 601/741 (81.11%), Query Frame = 0
Query: 1 MHFEISYIVYL--GGQKFGKKSSIFDVKLAKQSQFDILESVLESKVAVKESLIYSYSKYM 60
+H + SYIVYL G FG SI+DV+LA +S++DIL +V SK+A KES++Y+Y++ +
Sbjct: 22 VHTKKSYIVYLGSGSSTFGSNPSIYDVQLATESKYDILGTVKGSKMAAKESILYTYNRCI 81
Query: 61 NGFAALLDEKQVVDLEKNPSVVSVFENTIRHLHTTRSWHFLGMENDAGIPANSIWNAAKF 120
NGFAA+LD+ +V L KNPSVVSVFEN R LHTTRSW FLG+++ GIP NSIW A+KF
Sbjct: 82 NGFAAVLDDNEVTALAKNPSVVSVFENRERKLHTTRSWGFLGVDSGRGIPQNSIWKASKF 141
Query: 121 GEDVIIGNLDTGVWPESKSFDDTGYGPVPKRWMGECDGGANFTCNRKLIGARYFYQGIES 180
GEDVIIGNLDTGVWPES+SF D GYGPVP RWMG C+GG+ F CNRKLIGARYFY+G E
Sbjct: 142 GEDVIIGNLDTGVWPESESFHDAGYGPVPSRWMGACEGGSKFRCNRKLIGARYFYRGYEM 201
Query: 181 TNGPLNISMHTARDHEGHGSHTLSTAGGNLVPGANVFGSGNGTAKGGSPKARVVAYKVCW 240
NGPLNIS ARDHEGHG+HTLSTAGGN V GANVFG+GNGTAKGG+PKARV AYKVCW
Sbjct: 202 INGPLNISSLNARDHEGHGTHTLSTAGGNFVQGANVFGNGNGTAKGGAPKARVAAYKVCW 261
Query: 241 P-GGCSDADILAGFEAAMDDGVDVLSVSLGSRATQFATDPVSVASFHAVQQGIVVVCSAG 300
P G CSDAD+LAG EAA+ DGVDVLS+SLG+ A FA DP+SV +FHA+QQGI+VVCSAG
Sbjct: 262 PQGKCSDADLLAGIEAAISDGVDVLSISLGATAQDFANDPISVGAFHAIQQGIIVVCSAG 321
Query: 301 NNGPDPATVANIAPWMFTVAASTIDREFVSYVGLGNKKQIKGSSLSSEGLAVGSLHPLIN 360
N+GP P TV N++PWMFTV AS+IDR F SYV LGNKKQIKGSSLSS GL G L+PL+N
Sbjct: 322 NDGPLPGTVTNVSPWMFTVGASSIDRGFFSYVYLGNKKQIKGSSLSSGGLPRGKLYPLMN 381
Query: 361 AVDAKAANVPDKLAQLCEKDSLDPAKAKGKIVVCLCGDDENVDQGFEVRRAGGVGLILVN 420
+V+AKA+N D LAQLCE+ SLDP KA+GKI+VCL GD+ +D+ FEV R GGVG+ILVN
Sbjct: 382 SVNAKASNASDGLAQLCEEGSLDPMKARGKIIVCLRGDNGRMDKSFEVLRVGGVGMILVN 441
Query: 421 DEEDGADTISDPHFLPASHVSHEDGLLIAQYMKSTTTPEASISAVKTEIGVKPSPVMAVF 480
D+ G+ +DPH LP SHVS+ DGL IAQY+KST P A+I+ V+TEIG+KPSPVMA F
Sbjct: 442 DKISGSGIETDPHMLPTSHVSYIDGLSIAQYLKSTKRPVATITPVRTEIGIKPSPVMATF 501
Query: 481 SSRGPNPIEESMIKPDITAPGVTIIASMPEAVAASKSALDTRRVPFNILSGTSMACPHIS 540
SSRGPN I E++IKPDITAPGV IIAS A AA+ D RRVPFN+ SGTSM+CPHIS
Sbjct: 502 SSRGPNHITEAIIKPDITAPGVNIIASFTRAAAATDLPFDKRRVPFNVQSGTSMSCPHIS 561
Query: 541 GIAGLLRVLNPTWSPAAIRSAMMTTAITIDNTNHSMLNSNKADATPFDYGAGYVQPNNAM 600
G+AGLL+ L+PTWSPAAI+SA+MTTA T DNT ++ML+ NK ATPFDYGAG V PNNAM
Sbjct: 562 GVAGLLKKLHPTWSPAAIKSAIMTTAKTRDNTKNTMLDFNKVKATPFDYGAGLVHPNNAM 621
Query: 601 DPGLIYNTTIEDYLNFLCAKKYTDAELKKFSTQAFLCEESFAVTDLNYPSISIPKLAAGA 660
DPGL+Y+TTI+DYLNFLC + Y LKKFS + F+C ++FA+TDLNYPSIS+PKL GA
Sbjct: 622 DPGLVYDTTIDDYLNFLCTQGYNSLTLKKFSNKPFVCSKNFAITDLNYPSISVPKLQIGA 681
Query: 661 PVTINRRLLNVGSPGTYVAQVKVPESVQAKVEPSSLQFSKVDEEKSFKIVFQHVGE-RRV 720
PVT+NRR+ NVGS GTYVA+V++P+ + VEPS+LQF V EEK FK+VF + + RR
Sbjct: 682 PVTVNRRVKNVGSAGTYVARVRMPKGITVMVEPSTLQFHGVGEEKPFKLVFHYAQQVRRP 741
Query: 721 SYEFGTLEWSDGKHFVRSPLA 738
Y FG L WSDGKHFVRSP+A
Sbjct: 742 GYVFGALVWSDGKHFVRSPIA 762
BLAST of Sed0024525 vs. NCBI nr
Match:
XP_023005962.1 (uncharacterized protein LOC111498820 [Cucurbita maxima])
HSP 1 Score: 1015.4 bits (2624), Expect = 2.5e-292
Identity = 502/739 (67.93%), Postives = 605/739 (81.87%), Query Frame = 0
Query: 1 MHFEISYIVYLGGQKFGKKSSIFDVKLAKQSQFDILESVLESKVAVKESLIYSYSKYMNG 60
+H + SYIVYLG + SI+DV+LA +S++DIL +V SKVA KES++Y+Y++ +NG
Sbjct: 22 VHTKKSYIVYLGSE---SNPSIYDVQLATESKYDILGTVKGSKVAAKESILYTYNRCING 81
Query: 61 FAALLDEKQVVDLEKNPSVVSVFENTIRHLHTTRSWHFLGMENDAGIPANSIWNAAKFGE 120
FAA+LD+K+V L KNPSVVS+FEN R LHTTRSW FLG+ +D GIP NSIW A+KFGE
Sbjct: 82 FAAVLDDKEVTALAKNPSVVSIFENRERKLHTTRSWSFLGVGSDRGIPWNSIWKASKFGE 141
Query: 121 DVIIGNLDTGVWPESKSFDDTGYGPVPKRWMGECDGGANFTCNRKLIGARYFYQGIESTN 180
DVIIGNLDTGVWPES SF D+GYGPVP RWMG C+GG+NF+CNRKLIGARYFY+G E N
Sbjct: 142 DVIIGNLDTGVWPESHSFHDSGYGPVPSRWMGACEGGSNFSCNRKLIGARYFYRGYEMIN 201
Query: 181 GPLNISMHTARDHEGHGSHTLSTAGGNLVPGANVFGSGNGTAKGGSPKARVVAYKVCWP- 240
GPLNIS ARDHEGHG+HTLSTAGGN V GANVFG+GNGTAKGG+PKARV AYKVCWP
Sbjct: 202 GPLNISSLNARDHEGHGTHTLSTAGGNFVRGANVFGNGNGTAKGGAPKARVAAYKVCWPQ 261
Query: 241 GGCSDADILAGFEAAMDDGVDVLSVSLGSRATQFATDPVSVASFHAVQQGIVVVCSAGNN 300
G CSDAD+LAG EAA+ DGVDVLS+SLG+ A FA DP+SV +FHA+QQGI+VVCSAGN+
Sbjct: 262 GKCSDADLLAGIEAAISDGVDVLSISLGAAAQDFADDPISVGAFHAIQQGIIVVCSAGND 321
Query: 301 GPDPATVANIAPWMFTVAASTIDREFVSYVGLGNKKQIKGSSLSSEGLAVGSLHPLINAV 360
GP P TV N++PWMFTV AS+IDR F SYV LGNKKQ+KGSSLSS GL G L+PL+N+V
Sbjct: 322 GPLPGTVTNVSPWMFTVGASSIDRGFFSYVYLGNKKQVKGSSLSSGGLPRGKLYPLMNSV 381
Query: 361 DAKAANVPDKLAQLCEKDSLDPAKAKGKIVVCLCGDDENVDQGFEVRRAGGVGLILVNDE 420
+AKA+N D LAQLCE+ SLDP KA+GKI+VCL GD+ +D+ FEV R GGVG+ILVND+
Sbjct: 382 NAKASNASDGLAQLCEEGSLDPMKARGKIIVCLRGDNGRMDKSFEVLRVGGVGMILVNDK 441
Query: 421 EDGADTISDPHFLPASHVSHEDGLLIAQYMKSTTTPEASISAVKTEIGVKPSPVMAVFSS 480
G+D +D H LP SHVS+ DGL IA+Y+KST P ASI+ V+TEIG+KPSPVMA FSS
Sbjct: 442 ISGSDIETDAHMLPTSHVSYIDGLSIAEYLKSTKRPVASITPVRTEIGIKPSPVMAPFSS 501
Query: 481 RGPNPIEESMIKPDITAPGVTIIASMPEAVAASKSALDTRRVPFNILSGTSMACPHISGI 540
RGPN I E+MIKPDI+APGV IIAS +AVAA+ D RRVPFN+ SGTSM+CPHI+G+
Sbjct: 502 RGPNHIAEAMIKPDISAPGVNIIASFTKAVAATDLPFDKRRVPFNVQSGTSMSCPHIAGV 561
Query: 541 AGLLRVLNPTWSPAAIRSAMMTTAITIDNTNHSMLNSNKADATPFDYGAGYVQPNNAMDP 600
AGLL+ L+PTWSPAAI+SA+MTTA T DNT ++ML+ NK ATPFDYGAG+V PN+AMDP
Sbjct: 562 AGLLKKLHPTWSPAAIKSAIMTTAKTRDNTKNTMLDFNKVKATPFDYGAGHVHPNDAMDP 621
Query: 601 GLIYNTTIEDYLNFLCAKKYTDAELKKFSTQAFLCEESFAVTDLNYPSISIPKLAAGAPV 660
GL+Y+TTI+DYLNFLC + Y LKKFS + F+C ++FA TDLNYPSIS+PKL G PV
Sbjct: 622 GLVYDTTIDDYLNFLCMQGYNSLTLKKFSNKPFVCAKNFASTDLNYPSISVPKLQIGVPV 681
Query: 661 TINRRLLNVGSPGTYVAQVKVPESVQAKVEPSSLQFSKVDEEKSFKIVFQHVGE-RRVSY 720
T+NRR+ NVGS GTYVA+V++P+ + VEPS+LQF V EEK+FK+VF + + RR Y
Sbjct: 682 TVNRRVKNVGSAGTYVARVRMPKGITVMVEPSTLQFHSVGEEKAFKLVFHYAQKVRRPGY 741
Query: 721 EFGTLEWSDGKHFVRSPLA 738
FG L WSDGKHFVRSP+A
Sbjct: 742 VFGALVWSDGKHFVRSPIA 757
BLAST of Sed0024525 vs. NCBI nr
Match:
XP_038905648.1 (subtilisin-like protease SBT5.4 [Benincasa hispida])
HSP 1 Score: 1015.0 bits (2623), Expect = 3.3e-292
Identity = 500/736 (67.93%), Postives = 599/736 (81.39%), Query Frame = 0
Query: 6 SYIVYLGGQKFGKKSSIFDVKLAKQSQFDILESVLESKVAVKESLIYSYSKYMNGFAALL 65
SYIVYLG FG + IFDV+LA QSQ+DIL SV+ SK+ KES+IY+Y++++NGFAA+L
Sbjct: 23 SYIVYLGSHSFG-SNPIFDVQLATQSQYDILGSVMGSKMTAKESMIYTYNRHINGFAAVL 82
Query: 66 DEKQVVDLEKNPSVVSVFENTIRHLHTTRSWHFLGMENDAGIPANSIWNAAKFGEDVIIG 125
DE +V+ + +NP+VVSVFEN R LHTTR+W FLG+ENDAG+P+NSIW AA+FGED+IIG
Sbjct: 83 DENEVMAIAQNPNVVSVFENKKRQLHTTRTWGFLGLENDAGVPSNSIWKAARFGEDIIIG 142
Query: 126 NLDTGVWPESKSFDDTGYGPVPKRWMGECDGGANFTCNRKLIGARYFYQGIESTNGPLNI 185
NLDTGVWPESKSF D GYGPVP RWMG C+GG NFTCN+KLIGARYFY+G E+ NGP++
Sbjct: 143 NLDTGVWPESKSFSDAGYGPVPSRWMGVCEGGFNFTCNKKLIGARYFYKGFEAANGPIDD 202
Query: 186 SMHTARDHEGHGSHTLSTAGGNLVPGANVFGSGNGTAKGGSPKARVVAYKVCWP---GGC 245
S+ ARD EGHGSHTLSTAGGN VPGANVFG+GNGTAKGGSPKARV AYKVCWP GGC
Sbjct: 203 SVKFARDLEGHGSHTLSTAGGNFVPGANVFGNGNGTAKGGSPKARVAAYKVCWPALTGGC 262
Query: 246 SDADILAGFEAAMDDGVDVLSVSLGSRATQFATDPVSVASFHAVQQGIVVVCSAGNNGPD 305
DADILA FEAA+ DGVDVLSVSLGS A FA+DPV++ +FHAVQ+GI VVCSAGN+GP
Sbjct: 263 FDADILAAFEAAISDGVDVLSVSLGSPAQDFASDPVAIGAFHAVQEGITVVCSAGNDGPS 322
Query: 306 PATVANIAPWMFTVAASTIDREFVSYVGLGNKKQIKGSSLSSEGLAVGSLHPLINAVDAK 365
P+TV N+APWMFTVAAST+DR+F SYV LGNKKQ KG SLS+ G G+ +PL++ V K
Sbjct: 323 PSTVTNVAPWMFTVAASTVDRDFFSYVTLGNKKQFKGLSLSAGGTPGGNFYPLMDGVQVK 382
Query: 366 AANVPDKLAQLCEKDSLDPAKAKGKIVVCLCGDDENVDQGFEVRRAGGVGLILVNDEEDG 425
AAN+ D LAQLCE+ SLDP KAKGKI++CL GD+ +D+G EV R GGVG+ILVND+ DG
Sbjct: 383 AANITDNLAQLCEEGSLDPTKAKGKIILCLRGDNARLDKGVEVLRVGGVGMILVNDKLDG 442
Query: 426 ADTISDPHFLPASHVSHEDGLLIAQYMKSTTTPEASISAVKTEIGVKPSPVMAVFSSRGP 485
D +D HFLPASH+++ DGL I QY+ ST +P ASIS V+TE+G+KPSP+M FSSRGP
Sbjct: 443 NDVSADRHFLPASHLNYTDGLEIFQYLNSTQSPVASISHVETELGIKPSPMMGDFSSRGP 502
Query: 486 NPIEESMIKPDITAPGVTIIASMPEAVAASKSALDTRRVPFNILSGTSMACPHISGIAGL 545
NPI +SMIKPDITAPG+ IIA++ E+ A+ SA DTRRVPFN GTSM+CPHISG+ GL
Sbjct: 503 NPIIDSMIKPDITAPGLNIIAAVSESATATSSAYDTRRVPFNFEIGTSMSCPHISGVVGL 562
Query: 546 LRVLNPTWSPAAIRSAMMTTAITIDNTNHSMLNSNKADATPFDYGAGYVQPNNAMDPGLI 605
L+ L P WSPAAIRSA+MTTA T DN+ S+L+ NK ATPF YGAG+V PNNAMDPGL+
Sbjct: 563 LKTLYPKWSPAAIRSAIMTTAKTRDNSKKSILDYNKMKATPFHYGAGHVHPNNAMDPGLV 622
Query: 606 YNTTIEDYLNFLCAKKYTDAELKKFSTQAFLCEESFAVTDLNYPSISIPKLAAGAPVTIN 665
Y+TT EDY+NFLCA Y L++F + ++C ESFAV DLNYPSIS+PKL AG PVTIN
Sbjct: 623 YDTTTEDYMNFLCALGYNYTTLREFYNKPYVCPESFAVMDLNYPSISVPKLTAGVPVTIN 682
Query: 666 RRLLNVGSPGTYVAQVKVPESVQAKVEPSSLQFSKVDEEKSFKIVFQHVGE-RRVSYEFG 725
R++ NVGSPGTYVA+VKV V VEPS+LQF EEK+FK+VFQ+ G + + FG
Sbjct: 683 RKVKNVGSPGTYVARVKVSSMVSVTVEPSTLQFDSEGEEKAFKVVFQYKGTGQGKDHVFG 742
Query: 726 TLEWSDGKHFVRSPLA 738
TL WSDGKHFVRSP+A
Sbjct: 743 TLIWSDGKHFVRSPMA 757
BLAST of Sed0024525 vs. NCBI nr
Match:
KAG6596783.1 (Subtilisin-like protease 5.3, partial [Cucurbita argyrosperma subsp. sororia])
HSP 1 Score: 1014.2 bits (2621), Expect = 5.6e-292
Identity = 503/741 (67.88%), Postives = 600/741 (80.97%), Query Frame = 0
Query: 1 MHFEISYIVYL--GGQKFGKKSSIFDVKLAKQSQFDILESVLESKVAVKESLIYSYSKYM 60
+H + SYIVYL G FG SI+DV+LA +S++DIL +V SK+A KES++Y+Y++ +
Sbjct: 22 VHTKKSYIVYLGSGSSTFGSNPSIYDVQLATESKYDILGTVKGSKMAAKESILYTYNRCI 81
Query: 61 NGFAALLDEKQVVDLEKNPSVVSVFENTIRHLHTTRSWHFLGMENDAGIPANSIWNAAKF 120
NGFAA+LD+ +V L KNPSVVSVFEN R LHTTRSW FLG+++ GI NSIW A+KF
Sbjct: 82 NGFAAVLDDNEVTALAKNPSVVSVFENRERKLHTTRSWGFLGVDSGRGILQNSIWKASKF 141
Query: 121 GEDVIIGNLDTGVWPESKSFDDTGYGPVPKRWMGECDGGANFTCNRKLIGARYFYQGIES 180
GEDVIIGNLDTGVWPES+SF D GYGPVP RWMG C+GG+ F CNRKLIGARYFY+G E
Sbjct: 142 GEDVIIGNLDTGVWPESESFHDAGYGPVPPRWMGACEGGSKFRCNRKLIGARYFYRGYEM 201
Query: 181 TNGPLNISMHTARDHEGHGSHTLSTAGGNLVPGANVFGSGNGTAKGGSPKARVVAYKVCW 240
NGPLNIS ARDHEGHG+HTLSTAGGN V GANVFG+GNGTAKGG+PKARV AYKVCW
Sbjct: 202 INGPLNISSLNARDHEGHGTHTLSTAGGNFVQGANVFGNGNGTAKGGAPKARVAAYKVCW 261
Query: 241 P-GGCSDADILAGFEAAMDDGVDVLSVSLGSRATQFATDPVSVASFHAVQQGIVVVCSAG 300
P G CSDAD+LAG EAA+ DGVDVLS+SLG+ A FA DP+SV +FHA+QQGI+VVCSAG
Sbjct: 262 PQGKCSDADLLAGIEAAISDGVDVLSISLGATAQDFANDPISVGAFHAIQQGIIVVCSAG 321
Query: 301 NNGPDPATVANIAPWMFTVAASTIDREFVSYVGLGNKKQIKGSSLSSEGLAVGSLHPLIN 360
N+GP P TV N++PWMFTV AS+IDR F SYV LGNKKQIKGSSLSS GL G L+PL+N
Sbjct: 322 NDGPLPGTVTNVSPWMFTVGASSIDRGFFSYVYLGNKKQIKGSSLSSGGLPRGKLYPLMN 381
Query: 361 AVDAKAANVPDKLAQLCEKDSLDPAKAKGKIVVCLCGDDENVDQGFEVRRAGGVGLILVN 420
+V+AKA+N D LAQLCE+ SLDP KA+GKI+VCL GD+ +D+ FEV R GGVG+ILVN
Sbjct: 382 SVNAKASNASDGLAQLCEEGSLDPMKARGKIIVCLRGDNGRMDKSFEVLRVGGVGMILVN 441
Query: 421 DEEDGADTISDPHFLPASHVSHEDGLLIAQYMKSTTTPEASISAVKTEIGVKPSPVMAVF 480
D+ G+ +DPH LP SHVS+ DGL IAQY+KST P A+I+ V+TEIG+KPSPVMA F
Sbjct: 442 DKISGSGIETDPHMLPTSHVSYIDGLSIAQYLKSTKRPVATITPVRTEIGIKPSPVMATF 501
Query: 481 SSRGPNPIEESMIKPDITAPGVTIIASMPEAVAASKSALDTRRVPFNILSGTSMACPHIS 540
SSRGPN I E++IKPDITAPGV IIAS A AA+ D RRVPFN+ SGTSM+CPHIS
Sbjct: 502 SSRGPNHITEAIIKPDITAPGVNIIASFTRAAAATDLPFDKRRVPFNVQSGTSMSCPHIS 561
Query: 541 GIAGLLRVLNPTWSPAAIRSAMMTTAITIDNTNHSMLNSNKADATPFDYGAGYVQPNNAM 600
G+AGLL+ L+PTWSPAAI+SA+MTTA T DNT ++ML+ NK ATPFDYGAG V PNNAM
Sbjct: 562 GVAGLLKKLHPTWSPAAIKSAIMTTAKTRDNTKNTMLDFNKVKATPFDYGAGLVHPNNAM 621
Query: 601 DPGLIYNTTIEDYLNFLCAKKYTDAELKKFSTQAFLCEESFAVTDLNYPSISIPKLAAGA 660
DPGL+Y+TTI+DYLNFLC + Y LKKFS + F+C ++FA+TDLNYPSIS+PKL GA
Sbjct: 622 DPGLVYDTTIDDYLNFLCTQGYNSLTLKKFSNKPFVCAKNFAITDLNYPSISVPKLQIGA 681
Query: 661 PVTINRRLLNVGSPGTYVAQVKVPESVQAKVEPSSLQFSKVDEEKSFKIVFQHVGE-RRV 720
PVT+NRR+ NVGS GTYVA+V++P+ + VEPS+LQF V EEK FK+VF + + RR
Sbjct: 682 PVTVNRRVKNVGSAGTYVARVRMPKGITVMVEPSTLQFHSVGEEKPFKLVFHYAQKVRRP 741
Query: 721 SYEFGTLEWSDGKHFVRSPLA 738
Y FG L WSDGKHFVRSP+A
Sbjct: 742 GYVFGALVWSDGKHFVRSPIA 762
BLAST of Sed0024525 vs. ExPASy Swiss-Prot
Match:
Q9ZSP5 (Subtilisin-like protease SBT5.3 OS=Arabidopsis thaliana OX=3702 GN=AIR3 PE=2 SV=1)
HSP 1 Score: 735.7 bits (1898), Expect = 5.1e-211
Identity = 374/739 (50.61%), Postives = 503/739 (68.06%), Query Frame = 0
Query: 6 SYIVYLGGQKFGKKSSIFDVKLAKQSQFDILESVLESKVAVKESLIYSYSKYMNGFAALL 65
SY+VY G + + + K++ +D L S S+ +++ YSY+K++NGFAA L
Sbjct: 31 SYVVYFGAHSHVGEITEDAMDRVKETHYDFLGSFTGSRERATDAIFYSYTKHINGFAAHL 90
Query: 66 DEKQVVDLEKNPSVVSVFENTIRHLHTTRSWHFLGMENDAGIPANSIWNAAKFGEDVIIG 125
D ++ K+P VVSVF N LHTTRSW FLG+E+++ +P++SIW A+FGED II
Sbjct: 91 DHDLAYEISKHPEVVSVFPNKALKLHTTRSWDFLGLEHNSYVPSSSIWRKARFGEDTIIA 150
Query: 126 NLDTGVWPESKSFDDTGYGPVPKRWMGECDG--GANFTCNRKLIGARYFYQGIESTNGPL 185
NLDTGVWPESKSF D G GP+P RW G C A F CNRKLIGARYF +G + G L
Sbjct: 151 NLDTGVWPESKSFRDEGLGPIPSRWKGICQNQKDATFHCNRKLIGARYFNKGYAAAVGHL 210
Query: 186 NISMHTARDHEGHGSHTLSTAGGNLVPGANVFGSGNGTAKGGSPKARVVAYKVCWP---- 245
N S + RD +GHGSHTLSTA G+ VPG ++FG GNGTAKGGSP+ARV AYKVCWP
Sbjct: 211 NSSFDSPRDLDGHGSHTLSTAAGDFVPGVSIFGQGNGTAKGGSPRARVAAYKVCWPPVKG 270
Query: 246 GGCSDADILAGFEAAMDDGVDVLSVSLGSRATQFATDPVSVASFHAVQQGIVVVCSAGNN 305
C DAD+LA F+AA+ DG DV+SVSLG T F D V++ SFHA ++ IVVVCSAGN+
Sbjct: 271 NECYDADVLAAFDAAIHDGADVISVSLGGEPTSFFNDSVAIGSFHAAKKRIVVVCSAGNS 330
Query: 306 GPDPATVANIAPWMFTVAASTIDREFVSYVGLGNKKQIKGSSLSSEGLAVGSLHPLINAV 365
GP +TV+N+APW TV AST+DREF S + LGN K KG SLSS L +P++ +V
Sbjct: 331 GPADSTVSNVAPWQITVGASTMDREFASNLVLGNGKHYKGQSLSSTALPHAKFYPIMASV 390
Query: 366 DAKAANVPDKLAQLCEKDSLDPAKAKGKIVVCLCGDDENVDQGFEVRRAGGVGLILVNDE 425
+AKA N AQLC+ SLDP K KGKI+VCL G + V++G V GG+G++L N
Sbjct: 391 NAKAKNASALDAQLCKLGSLDPIKTKGKILVCLRGQNGRVEKGRAVALGGGIGMVLENTY 450
Query: 426 EDGADTISDPHFLPASHVSHEDGLLIAQYMKSTTTPEASISAVKTEIGVKPSPVMAVFSS 485
G D ++DPH LPA+ ++ +D +++Y+ T P A I+ +T++G+KP+PVMA FSS
Sbjct: 451 VTGNDLLADPHVLPATQLTSKDSFAVSRYISQTKKPIAHITPSRTDLGLKPAPVMASFSS 510
Query: 486 RGPNPIEESMIKPDITAPGVTIIASMPEAVAASKSALDTRRVPFNILSGTSMACPHISGI 545
+GP+ + ++KPDITAPGV++IA+ AV+ + D RR+ FN +SGTSM+CPHISGI
Sbjct: 511 KGPSIVAPQILKPDITAPGVSVIAAYTGAVSPTNEQFDPRRLLFNAISGTSMSCPHISGI 570
Query: 546 AGLLRVLNPTWSPAAIRSAMMTTAITIDNTNHSMLNSNKADATPFDYGAGYVQPNNAMDP 605
AGLL+ P+WSPAAIRSA+MTTA +D+ + N+ ATPF +GAG+VQPN A++P
Sbjct: 571 AGLLKTRYPSWSPAAIRSAIMTTATIMDDIPGPIQNATNMKATPFSFGAGHVQPNLAVNP 630
Query: 606 GLIYNTTIEDYLNFLCAKKYTDAELKKFSTQAFLCEE-SFAVTDLNYPSISIPKLAAGAP 665
GL+Y+ I+DYLNFLC+ Y +++ FS F C ++ +LNYPSI++P L + +
Sbjct: 631 GLVYDLGIKDYLNFLCSLGYNASQISVFSGNNFTCSSPKISLVNLNYPSITVPNLTS-SK 690
Query: 666 VTINRRLLNVGSPGTYVAQVKVPESVQAKVEPSSLQFSKVDEEKSFKIVF-QHVGERRVS 725
VT++R + NVG P Y +V P+ V V+P+SL F+KV E+K+FK++ + G
Sbjct: 691 VTVSRTVKNVGRPSMYTVKVNNPQGVYVAVKPTSLNFTKVGEQKTFKVILVKSKGNVAKG 750
Query: 726 YEFGTLEWSDGKHFVRSPL 737
Y FG L WSD KH VRSP+
Sbjct: 751 YVFGELVWSDKKHRVRSPI 768
BLAST of Sed0024525 vs. ExPASy Swiss-Prot
Match:
F4JXC5 (Subtilisin-like protease SBT5.4 OS=Arabidopsis thaliana OX=3702 GN=SBT5.4 PE=1 SV=1)
HSP 1 Score: 716.5 bits (1848), Expect = 3.2e-205
Identity = 373/737 (50.61%), Postives = 481/737 (65.26%), Query Frame = 0
Query: 6 SYIVYLGGQKFGKKSSIFDVKLAKQSQFDILESVLESKVAVKESLIYSYSKYMNGFAALL 65
SYIVYLG + S + S L S + S KE++ YSY +++NGFAA+L
Sbjct: 41 SYIVYLGSHAHLPQISSAHLDGVAHSHRTFLASFVGSHENAKEAIFYSYKRHINGFAAIL 100
Query: 66 DEKQVVDLEKNPSVVSVFENTIRHLHTTRSWHFLGMENDAGIPANSIWNAAKFGEDVIIG 125
DE + ++ K+P VVSVF N R LHTT SW+F+ + + + +S+WN A +GED II
Sbjct: 101 DENEAAEIAKHPDVVSVFPNKGRKLHTTHSWNFMLLAKNGVVHKSSLWNKAGYGEDTIIA 160
Query: 126 NLDTGVWPESKSFDDTGYGPVPKRWMGECDGGANFTCNRKLIGARYFYQGIESTNG-PLN 185
NLDTGVWPESKSF D GYG VP RW G C + CNRKLIGARYF +G + G P N
Sbjct: 161 NLDTGVWPESKSFSDEGYGAVPARWKGRCH--KDVPCNRKLIGARYFNKGYLAYTGLPSN 220
Query: 186 ISMHTARDHEGHGSHTLSTAGGNLVPGANVFGSGNGTAKGGSPKARVVAYKVCWP----G 245
S T RDH+GHGSHTLSTA GN VPGANVFG GNGTA GGSPKARV AYKVCWP
Sbjct: 221 ASYETCRDHDGHGSHTLSTAAGNFVPGANVFGIGNGTASGGSPKARVAAYKVCWPPVDGA 280
Query: 246 GCSDADILAGFEAAMDDGVDVLSVSLGSRATQFATDPVSVASFHAVQQGIVVVCSAGNNG 305
C DADILA EAA++DGVDVLS S+G A + +D +++ SFHAV+ G+ VVCSAGN+G
Sbjct: 281 ECFDADILAAIEAAIEDGVDVLSASVGGDAGDYMSDGIAIGSFHAVKNGVTVVCSAGNSG 340
Query: 306 PDPATVANIAPWMFTVAASTIDREFVSYVGLGNKKQIKGSSLSSEGLAVGSLHPLINAVD 365
P TV+N+APW+ TV AS++DREF ++V L N + KG+SL S+ L ++ LI+A D
Sbjct: 341 PKSGTVSNVAPWVITVGASSMDREFQAFVELKNGQSFKGTSL-SKPLPEEKMYSLISAAD 400
Query: 366 AKAANVPDKLAQLCEKDSLDPAKAKGKIVVCLCGDDENVDQGFEVRRAGGVGLILVNDEE 425
A AN A LC+K SLDP K KGKI+VCL GD+ VD+G + AG G++L ND+
Sbjct: 401 ANVANGNVTDALLCKKGSLDPKKVKGKILVCLRGDNARVDKGMQAAAAGAAGMVLCNDKA 460
Query: 426 DGADTISDPHFLPASHVSHEDGLLIAQYMKSTTTPEASISAVKTEIGVKPSPVMAVFSSR 485
G + ISD H LPAS + ++DG + Y+ ST P+ I A + KP+P MA FSSR
Sbjct: 461 SGNEIISDAHVLPASQIDYKDGETLFSYLSSTKDPKGYIKAPTATLNTKPAPFMASFSSR 520
Query: 486 GPNPIEESMIKPDITAPGVTIIASMPEAVAASKSALDTRRVPFNILSGTSMACPHISGIA 545
GPN I ++KPDITAPGV IIA+ EA + D RR PFN SGTSM+CPHISG+
Sbjct: 521 GPNTITPGILKPDITAPGVNIIAAFTEATGPTDLDSDNRRTPFNTESGTSMSCPHISGVV 580
Query: 546 GLLRVLNPTWSPAAIRSAMMTTAITIDNTNHSMLNSNKADATPFDYGAGYVQPNNAMDPG 605
GLL+ L+P WSPAAIRSA+MTT+ T +N M++ + A PF YG+G+VQPN A PG
Sbjct: 581 GLLKTLHPHWSPAAIRSAIMTTSRTRNNRRKPMVDESFKKANPFSYGSGHVQPNKAAHPG 640
Query: 606 LIYNTTIEDYLNFLCAKKYTDAELKKFSTQ-AFLCEESFAVTDLNYPSISIPKLAAGAPV 665
L+Y+ T DYL+FLCA Y + ++ F+ + C + + D NYPSI++P L +
Sbjct: 641 LVYDLTTGDYLDFLCAVGYNNTVVQLFAEDPQYTCRQGANLLDFNYPSITVPNLTGS--I 700
Query: 666 TINRRLLNVGSPGTYVAQVKVPESVQAKVEPSSLQFSKVDEEKSFKIVFQHVGERRVSYE 725
T+ R+L NVG P TY A+ + P V+ VEP L F+K E K F++ + + Y
Sbjct: 701 TVTRKLKNVGPPATYNARFREPLGVRVSVEPKQLTFNKTGEVKIFQMTLRPLPVTPSGYV 760
Query: 726 FGTLEWSDGKHFVRSPL 737
FG L W+D H+VRSP+
Sbjct: 761 FGELTWTDSHHYVRSPI 772
BLAST of Sed0024525 vs. ExPASy Swiss-Prot
Match:
I1N462 (Subtilisin-like protease Glyma18g48580 OS=Glycine max OX=3847 GN=Glyma18g48580 PE=1 SV=3)
HSP 1 Score: 671.8 bits (1732), Expect = 9.0e-192
Identity = 365/759 (48.09%), Postives = 487/759 (64.16%), Query Frame = 0
Query: 7 YIVYLGGQKFGKKSSIFDVKLAKQSQFDILESVLESKVAVKESLIYSYSKYMNGFAALLD 66
YIVY+G G + D++LA S +D+L S+ S+ KE++IYSY++++NGFAALL+
Sbjct: 32 YIVYMGAHSHGPSPTSADLELATDSHYDLLGSIFGSREKAKEAIIYSYNRHINGFAALLE 91
Query: 67 EKQVVDLEKNPSVVSVFENTIRHLHTTRSWHFLGMENDAGIPANSIWNAAKFGEDVIIGN 126
E++ D+ KNP+VVSVF + LHTTRSW FLG+ NS W +FGE+ IIGN
Sbjct: 92 EEEAADIAKNPNVVSVFLSKEHKLHTTRSWEFLGLHRRG---QNSAWQKGRFGENTIIGN 151
Query: 127 LDTGVWPESKSFDDTGYGPVPKRWMG------ECDGGANFTCNRKLIGARYFYQGIESTN 186
+DTGVWPES+SF D GYG VP +W G + G TCNRKLIGARY+ + E+ N
Sbjct: 152 IDTGVWPESQSFSDKGYGTVPSKWRGGLCQINKLPGSMKNTCNRKLIGARYYNKAFEAHN 211
Query: 187 GPLNISMHTARDHEGHGSHTLSTAGGNLVPGANVFGSGNGTAKGGSPKARVVAYKVCW-- 246
G L+ +HTARD GHG+HTLSTAGGN VPGA VF GNGTAKGGSP+ARV AYKVCW
Sbjct: 212 GQLDPLLHTARDFVGHGTHTLSTAGGNFVPGARVFAVGNGTAKGGSPRARVAAYKVCWSL 271
Query: 247 --PGGCSDADILAGFEAAMDDGVDVLSVSLG----SRATQFATDPVSVASFHAVQQGIVV 306
P C AD+LA + A+DDGVDV++VS G A TD +S+ +FHA+ + I++
Sbjct: 272 TDPASCYGADVLAAIDQAIDDGVDVINVSFGVSYVVTAEGIFTDEISIGAFHAISKNILL 331
Query: 307 VCSAGNNGPDPATVANIAPWMFTVAASTIDREFVSYVGLGNKKQIKGSSLSSEGLAVGSL 366
V SAGN+GP P TVAN+APW+FT+AAST+DR+F S + + N + I+G+SL L
Sbjct: 332 VASAGNDGPTPGTVANVAPWVFTIAASTLDRDFSSNLTI-NNQLIEGASL-FVNLPPNQA 391
Query: 367 HPLINAVDAKAANVPDKLAQLCEKDSLDPAKAKGKIVVCLC-GDDENVDQGFEVRRAGGV 426
LI + DAK AN + AQLC + +LD K GKIV+C G ++V +G E AG
Sbjct: 392 FSLILSTDAKLANATFRDAQLCRRGTLDRTKVNGKIVLCTREGKIKSVAEGLEALTAGAR 451
Query: 427 GLILVNDEEDGADTISDPHFLPASHVSHEDGLLIAQYMKST----------TTPEASISA 486
G+IL N ++G ++PH + +K+T T +S
Sbjct: 452 GMILNNQMQNGKTLSAEPHVFSTVNTPPRRAKSRPHGVKTTAIGDEDDPLKTGDTIKMSR 511
Query: 487 VKTEIGVKPSPVMAVFSSRGPNPIEESMIKPDITAPGVTIIASMPEAVAASKSALDTRR- 546
+T G KP+PVMA FSSRGPN I+ S++KPD+TAPGV I+A+ E +AS +D RR
Sbjct: 512 ARTLFGRKPAPVMASFSSRGPNKIQPSILKPDVTAPGVNILAAYSEFASASSLLVDNRRG 571
Query: 547 VPFNILSGTSMACPHISGIAGLLRVLNPTWSPAAIRSAMMTTAITIDNTNHSMLNS-NKA 606
FN+L GTSM+CPH SGIAGLL+ +P+WSPAAI+SA+MTTA T+DNTN + ++ +K
Sbjct: 572 FKFNVLQGTSMSCPHASGIAGLLKTRHPSWSPAAIKSAIMTTATTLDNTNRPIQDAFDKT 631
Query: 607 DATPFDYGAGYVQPNNAMDPGLIYNTTIEDYLNFLCAKKYTDAELKKFS-TQAFLCEESF 666
A F YG+G+V+P+ A++PGL+Y+ ++ DYLNFLCA Y + + + F+C S
Sbjct: 632 LADAFAYGSGHVRPDLAIEPGLVYDLSLTDYLNFLCASGYDQQLISALNFNRTFICSGSH 691
Query: 667 AVTDLNYPSISIPKLAAGAPVTINRRLLNVGSPGTYVAQVKVPESVQAKVEPSSLQFSKV 726
+V DLNYPSI++P L PVTI R + NVG P TY + P V P SL F+K+
Sbjct: 692 SVNDLNYPSITLPNLRL-KPVTIARTVTNVGPPSTYTVSTRSPNGYSIAVVPPSLTFTKI 751
Query: 727 DEEKSFKIVFQ-HVGERRVSYEFGTLEWSDGKHFVRSPL 737
E K+FK++ Q R YEFG L W+DGKH VRSP+
Sbjct: 752 GERKTFKVIVQASSAATRRKYEFGDLRWTDGKHIVRSPI 784
BLAST of Sed0024525 vs. ExPASy Swiss-Prot
Match:
O65351 (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 SV=1)
HSP 1 Score: 579.7 bits (1493), Expect = 4.7e-164
Identity = 319/720 (44.31%), Postives = 450/720 (62.50%), Query Frame = 0
Query: 31 SQFDILESVLES---KVAVKESLIYSYSKYMNGFAALLDEKQVVDLEKNPSVVSVFENTI 90
S FD+ + +S ++ L+Y+Y ++GF+ L +++ L P V+SV
Sbjct: 43 SSFDLHSNWYDSSLRSISDSAELLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHR 102
Query: 91 RHLHTTRSWHFLGMENDAGIPANSIWNAAKFGEDVIIGNLDTGVWPESKSFDDTGYGPVP 150
LHTTR+ FLG++ ++ A DV++G LDTGVWPESKS+ D G+GP+P
Sbjct: 103 YELHTTRTPLFLGLDEHTA----DLFPEAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIP 162
Query: 151 KRWMGECDGGANFT---CNRKLIGARYFYQGIESTNGPLNIS--MHTARDHEGHGSHTLS 210
W G C+ G NFT CNRKLIGAR+F +G EST GP++ S + RD +GHG+HT S
Sbjct: 163 SSWKGGCEAGTNFTASLCNRKLIGARFFARGYESTMGPIDESKESRSPRDDDGHGTHTSS 222
Query: 211 TAGGNLVPGANVFGSGNGTAKGGSPKARVVAYKVCWPGGCSDADILAGFEAAMDDGVDVL 270
TA G++V GA++ G +GTA+G +P+ARV YKVCW GGC +DILA + A+ D V+VL
Sbjct: 223 TAAGSVVEGASLLGYASGTARGMAPRARVAVYKVCWLGGCFSSDILAAIDKAIADNVNVL 282
Query: 271 SVSLGSRATQFATDPVSVASFHAVQQGIVVVCSAGNNGPDPATVANIAPWMFTVAASTID 330
S+SLG + + D V++ +F A+++GI+V CSAGN GP ++++N+APW+ TV A T+D
Sbjct: 283 SMSLGGGMSDYYRDGVAIGAFAAMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLD 342
Query: 331 REFVSYVGLGNKKQIKGSSLSSEGLAVGSLHPLINAVDAKAANVPDKLAQLCEKDSLDPA 390
R+F + LGN K G SL L P I A +A A LC +L P
Sbjct: 343 RDFPALAILGNGKNFTGVSLFKGEALPDKLLPFIYAGNASNAT----NGNLCMTGTLIPE 402
Query: 391 KAKGKIVVCLCGDDENVDQGFEVRRAGGVGLILVNDEEDGADTISDPHFLPASHVSHEDG 450
K KGKIV+C G + V +G V+ AGGVG+IL N +G + ++D H LPA+ V + G
Sbjct: 403 KVKGKIVMCDRGINARVQKGDVVKAAGGVGMILANTAANGEELVADAHLLPATTVGEKAG 462
Query: 451 LLIAQYMKSTTTPEASISAVKTEIGVKPSPVMAVFSSRGPNPIEESMIKPDITAPGVTII 510
+I Y+ + P ASIS + T +GVKPSPV+A FSSRGPN I +++KPD+ APGV I+
Sbjct: 463 DIIRHYVTTDPNPTASISILGTVVGVKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNIL 522
Query: 511 ASMPEAVAASKSALDTRRVPFNILSGTSMACPHISGIAGLLRVLNPTWSPAAIRSAMMTT 570
A+ A + A D+RRV FNI+SGTSM+CPH+SG+A LL+ ++P WSPAAIRSA+MTT
Sbjct: 523 AAWTGAAGPTGLASDSRRVEFNIISGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTT 582
Query: 571 AITIDNTNHSMLN-SNKADATPFDYGAGYVQPNNAMDPGLIYNTTIEDYLNFLCAKKYTD 630
A +L+ + +TPFD+GAG+V P A +PGLIY+ T EDYL FLCA YT
Sbjct: 583 AYKTYKDGKPLLDIATGKPSTPFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTS 642
Query: 631 AELKKFSTQAFLCE--ESFAVTDLNYPSISIPKLAAGAPVTINRRLLNVGSPGTYVAQV- 690
+++ S + + C+ +S++V DLNYPS ++ GA R + +VG GTY +V
Sbjct: 643 PQIRSVSRRNYTCDPSKSYSVADLNYPSFAVNVDGVGA-YKYTRTVTSVGGAGTYSVKVT 702
Query: 691 KVPESVQAKVEPSSLQFSKVDEEKSFKIVFQ-HVGERRVSYEFGTLEWSDGKHFVRSPLA 738
V+ VEP+ L F + +E+KS+ + F + S FG++EWSDGKH V SP+A
Sbjct: 703 SETTGVKISVEPAVLNFKEANEKKSYTVTFTVDSSKPSGSNSFGSIEWSDGKHVVGSPVA 753
BLAST of Sed0024525 vs. ExPASy Swiss-Prot
Match:
Q9LUM3 (Subtilisin-like protease SBT1.5 OS=Arabidopsis thaliana OX=3702 GN=SBT1.5 PE=2 SV=1)
HSP 1 Score: 539.7 bits (1389), Expect = 5.3e-152
Identity = 314/758 (41.42%), Postives = 439/758 (57.92%), Query Frame = 0
Query: 5 ISYIVYLGGQKFGKKSSIFDVKLAKQSQFDILESVLESKVAVKESLIYSYSKYMNGFAAL 64
++YIV++ + K SIF + F S L S + S+I++Y +GF+A
Sbjct: 26 LTYIVHVDHE---AKPSIF------PTHFHWYTSSLASLTSSPPSIIHTYDTVFHGFSAR 85
Query: 65 LDEKQVVDLEKNPSVVSVFENTIRHLHTTRSWHFLGME--NDAGIPANSIWNAAKFGEDV 124
L + L +P V+SV +RHLHTTRS FLG+ + AG+ S FG D+
Sbjct: 86 LTSQDASQLLDHPHVISVIPEQVRHLHTTRSPEFLGLRSTDKAGLLEES-----DFGSDL 145
Query: 125 IIGNLDTGVWPESKSFDDTGYGPVPKRWMGECDGGANF---TCNRKLIGARYFYQGIEST 184
+IG +DTGVWPE SFDD G GPVP +W G+C +F CNRKL+GAR+F G E+T
Sbjct: 146 VIGVIDTGVWPERPSFDDRGLGPVPIKWKGQCIASQDFPESACNRKLVGARFFCGGYEAT 205
Query: 185 NGPLN--ISMHTARDHEGHGSHTLSTAGGNLVPGANVFGSGNGTAKGGSPKARVVAYKVC 244
NG +N + RD +GHG+HT S + G V A+ G +G A G +PKAR+ AYKVC
Sbjct: 206 NGKMNETTEFRSPRDSDGHGTHTASISAGRYVFPASTLGYAHGVAAGMAPKARLAAYKVC 265
Query: 245 WPGGCSDADILAGFEAAMDDGVDVLSVSLGSRATQFATDPVSVASFHAVQQGIVVVCSAG 304
W GC D+DILA F+ A+ DGVDV+S+S+G + D +++ +F A+ +GI V SAG
Sbjct: 266 WNSGCYDSDILAAFDTAVADGVDVISLSVGGVVVPYYLDAIAIGAFGAIDRGIFVSASAG 325
Query: 305 NNGPDPATVANIAPWMFTVAASTIDREFVSYVGLGNKKQIKGSSL-SSEGLAVGSLHPLI 364
N GP TV N+APWM TV A TIDR+F + V LGN K I G S+ GL G ++PL+
Sbjct: 326 NGGPGALTVTNVAPWMTTVGAGTIDRDFPANVKLGNGKMISGVSVYGGPGLDPGRMYPLV 385
Query: 365 NAVDAKAANVPDKLAQLCEKDSLDPAKAKGKIVVCLCGDDENVDQGFEVRRAGGVGLILV 424
+ + LC + SLDP KGKIV+C G + +G VR+ GG+G+I+
Sbjct: 386 YGGSLLGGD--GYSSSLCLEGSLDPNLVKGKIVLCDRGINSRATKGEIVRKNGGLGMIIA 445
Query: 425 NDEEDGADTISDPHFLPASHVSHEDGLLIAQYM------KSTTTPEASISAVKTEIGVKP 484
N DG ++D H LPA+ V G I +Y+ +S+ P A+I T +G++P
Sbjct: 446 NGVFDGEGLVADCHVLPATSVGASGGDEIRRYISESSKSRSSKHPTATIVFKGTRLGIRP 505
Query: 485 SPVMAVFSSRGPNPIEESMIKPDITAPGVTIIASMPEAVAASKSALDTRRVPFNILSGTS 544
+PV+A FS+RGPNP ++KPD+ APG+ I+A+ P+ + S D RR FNILSGTS
Sbjct: 506 APVVASFSARGPNPETPEILKPDVIAPGLNILAAWPDRIGPSGVTSDNRRTEFNILSGTS 565
Query: 545 MACPHISGIAGLLRVLNPTWSPAAIRSAMMTTAITIDNTNHSMLNSNKAD-ATPFDYGAG 604
MACPH+SG+A LL+ +P WSPAAIRSA++TTA T+DN+ M++ + + ++ DYG+G
Sbjct: 566 MACPHVSGLAALLKAAHPDWSPAAIRSALITTAYTVDNSGEPMMDESTGNTSSVMDYGSG 625
Query: 605 YVQPNNAMDPGLIYNTTIEDYLNFLCAKKYTDAELKKFSTQAFLCE---ESFAVTDLNYP 664
+V P AMDPGL+Y+ T DY+NFLC YT + + + C+ + V +LNYP
Sbjct: 626 HVHPTKAMDPGLVYDITSYDYINFLCNSNYTRTNIVTITRRQADCDGARRAGHVGNLNYP 685
Query: 665 SISIPKLAAG---APVTINRRLLNVG-SPGTYVAQVKVPESVQAKVEPSSLQFSKVDEEK 724
S S+ G R + NVG S Y +++ P VEP L F +V ++
Sbjct: 686 SFSVVFQQYGESKMSTHFIRTVTNVGDSDSVYEIKIRPPRGTTVTVEPEKLSFRRVGQKL 745
Query: 725 SF----KIVFQHVGERRVSYEFGTLEWSDGKHFVRSPL 737
SF K + + E G + WSDGK V SPL
Sbjct: 746 SFVVRVKTTEVKLSPGATNVETGHIVWSDGKRNVTSPL 767
BLAST of Sed0024525 vs. ExPASy TrEMBL
Match:
A0A6J1GDR2 (subtilisin-like protease SBT5.3 OS=Cucurbita moschata OX=3662 GN=LOC111453063 PE=3 SV=1)
HSP 1 Score: 1018.5 bits (2632), Expect = 1.4e-293
Identity = 504/741 (68.02%), Postives = 601/741 (81.11%), Query Frame = 0
Query: 1 MHFEISYIVYL--GGQKFGKKSSIFDVKLAKQSQFDILESVLESKVAVKESLIYSYSKYM 60
+H + SYIVYL G FG SI+DV+LA +S++DIL +V SK+A KES++Y+Y++ +
Sbjct: 22 VHTKKSYIVYLGSGSSTFGSNPSIYDVQLATESKYDILGTVKGSKMAAKESILYTYNRCI 81
Query: 61 NGFAALLDEKQVVDLEKNPSVVSVFENTIRHLHTTRSWHFLGMENDAGIPANSIWNAAKF 120
NGFAA+LD+ +V L KNPSVVSVFEN R LHTTRSW FLG+++ GIP NSIW A+KF
Sbjct: 82 NGFAAVLDDNEVTALAKNPSVVSVFENRERKLHTTRSWGFLGVDSGRGIPQNSIWKASKF 141
Query: 121 GEDVIIGNLDTGVWPESKSFDDTGYGPVPKRWMGECDGGANFTCNRKLIGARYFYQGIES 180
GEDVIIGNLDTGVWPES+SF D GYGPVP RWMG C+GG+ F CNRKLIGARYFY+G E
Sbjct: 142 GEDVIIGNLDTGVWPESESFHDAGYGPVPSRWMGACEGGSKFRCNRKLIGARYFYRGYEM 201
Query: 181 TNGPLNISMHTARDHEGHGSHTLSTAGGNLVPGANVFGSGNGTAKGGSPKARVVAYKVCW 240
NGPLNIS ARDHEGHG+HTLSTAGGN V GANVFG+GNGTAKGG+PKARV AYKVCW
Sbjct: 202 INGPLNISSLNARDHEGHGTHTLSTAGGNFVQGANVFGNGNGTAKGGAPKARVAAYKVCW 261
Query: 241 P-GGCSDADILAGFEAAMDDGVDVLSVSLGSRATQFATDPVSVASFHAVQQGIVVVCSAG 300
P G CSDAD+LAG EAA+ DGVDVLS+SLG+ A FA DP+SV +FHA+QQGI+VVCSAG
Sbjct: 262 PQGKCSDADLLAGIEAAISDGVDVLSISLGATAQDFANDPISVGAFHAIQQGIIVVCSAG 321
Query: 301 NNGPDPATVANIAPWMFTVAASTIDREFVSYVGLGNKKQIKGSSLSSEGLAVGSLHPLIN 360
N+GP P TV N++PWMFTV AS+IDR F SYV LGNKKQIKGSSLSS GL G L+PL+N
Sbjct: 322 NDGPLPGTVTNVSPWMFTVGASSIDRGFFSYVYLGNKKQIKGSSLSSGGLPRGKLYPLMN 381
Query: 361 AVDAKAANVPDKLAQLCEKDSLDPAKAKGKIVVCLCGDDENVDQGFEVRRAGGVGLILVN 420
+V+AKA+N D LAQLCE+ SLDP KA+GKI+VCL GD+ +D+ FEV R GGVG+ILVN
Sbjct: 382 SVNAKASNASDGLAQLCEEGSLDPMKARGKIIVCLRGDNGRMDKSFEVLRVGGVGMILVN 441
Query: 421 DEEDGADTISDPHFLPASHVSHEDGLLIAQYMKSTTTPEASISAVKTEIGVKPSPVMAVF 480
D+ G+ +DPH LP SHVS+ DGL IAQY+KST P A+I+ V+TEIG+KPSPVMA F
Sbjct: 442 DKISGSGIETDPHMLPTSHVSYIDGLSIAQYLKSTKRPVATITPVRTEIGIKPSPVMATF 501
Query: 481 SSRGPNPIEESMIKPDITAPGVTIIASMPEAVAASKSALDTRRVPFNILSGTSMACPHIS 540
SSRGPN I E++IKPDITAPGV IIAS A AA+ D RRVPFN+ SGTSM+CPHIS
Sbjct: 502 SSRGPNHITEAIIKPDITAPGVNIIASFTRAAAATDLPFDKRRVPFNVQSGTSMSCPHIS 561
Query: 541 GIAGLLRVLNPTWSPAAIRSAMMTTAITIDNTNHSMLNSNKADATPFDYGAGYVQPNNAM 600
G+AGLL+ L+PTWSPAAI+SA+MTTA T DNT ++ML+ NK ATPFDYGAG V PNNAM
Sbjct: 562 GVAGLLKKLHPTWSPAAIKSAIMTTAKTRDNTKNTMLDFNKVKATPFDYGAGLVHPNNAM 621
Query: 601 DPGLIYNTTIEDYLNFLCAKKYTDAELKKFSTQAFLCEESFAVTDLNYPSISIPKLAAGA 660
DPGL+Y+TTI+DYLNFLC + Y LKKFS + F+C ++FA+TDLNYPSIS+PKL GA
Sbjct: 622 DPGLVYDTTIDDYLNFLCTQGYNSLTLKKFSNKPFVCSKNFAITDLNYPSISVPKLQIGA 681
Query: 661 PVTINRRLLNVGSPGTYVAQVKVPESVQAKVEPSSLQFSKVDEEKSFKIVFQHVGE-RRV 720
PVT+NRR+ NVGS GTYVA+V++P+ + VEPS+LQF V EEK FK+VF + + RR
Sbjct: 682 PVTVNRRVKNVGSAGTYVARVRMPKGITVMVEPSTLQFHGVGEEKPFKLVFHYAQQVRRP 741
Query: 721 SYEFGTLEWSDGKHFVRSPLA 738
Y FG L WSDGKHFVRSP+A
Sbjct: 742 GYVFGALVWSDGKHFVRSPIA 762
BLAST of Sed0024525 vs. ExPASy TrEMBL
Match:
A0A6J1KUL8 (uncharacterized protein LOC111498820 OS=Cucurbita maxima OX=3661 GN=LOC111498820 PE=3 SV=1)
HSP 1 Score: 1015.4 bits (2624), Expect = 1.2e-292
Identity = 502/739 (67.93%), Postives = 605/739 (81.87%), Query Frame = 0
Query: 1 MHFEISYIVYLGGQKFGKKSSIFDVKLAKQSQFDILESVLESKVAVKESLIYSYSKYMNG 60
+H + SYIVYLG + SI+DV+LA +S++DIL +V SKVA KES++Y+Y++ +NG
Sbjct: 22 VHTKKSYIVYLGSE---SNPSIYDVQLATESKYDILGTVKGSKVAAKESILYTYNRCING 81
Query: 61 FAALLDEKQVVDLEKNPSVVSVFENTIRHLHTTRSWHFLGMENDAGIPANSIWNAAKFGE 120
FAA+LD+K+V L KNPSVVS+FEN R LHTTRSW FLG+ +D GIP NSIW A+KFGE
Sbjct: 82 FAAVLDDKEVTALAKNPSVVSIFENRERKLHTTRSWSFLGVGSDRGIPWNSIWKASKFGE 141
Query: 121 DVIIGNLDTGVWPESKSFDDTGYGPVPKRWMGECDGGANFTCNRKLIGARYFYQGIESTN 180
DVIIGNLDTGVWPES SF D+GYGPVP RWMG C+GG+NF+CNRKLIGARYFY+G E N
Sbjct: 142 DVIIGNLDTGVWPESHSFHDSGYGPVPSRWMGACEGGSNFSCNRKLIGARYFYRGYEMIN 201
Query: 181 GPLNISMHTARDHEGHGSHTLSTAGGNLVPGANVFGSGNGTAKGGSPKARVVAYKVCWP- 240
GPLNIS ARDHEGHG+HTLSTAGGN V GANVFG+GNGTAKGG+PKARV AYKVCWP
Sbjct: 202 GPLNISSLNARDHEGHGTHTLSTAGGNFVRGANVFGNGNGTAKGGAPKARVAAYKVCWPQ 261
Query: 241 GGCSDADILAGFEAAMDDGVDVLSVSLGSRATQFATDPVSVASFHAVQQGIVVVCSAGNN 300
G CSDAD+LAG EAA+ DGVDVLS+SLG+ A FA DP+SV +FHA+QQGI+VVCSAGN+
Sbjct: 262 GKCSDADLLAGIEAAISDGVDVLSISLGAAAQDFADDPISVGAFHAIQQGIIVVCSAGND 321
Query: 301 GPDPATVANIAPWMFTVAASTIDREFVSYVGLGNKKQIKGSSLSSEGLAVGSLHPLINAV 360
GP P TV N++PWMFTV AS+IDR F SYV LGNKKQ+KGSSLSS GL G L+PL+N+V
Sbjct: 322 GPLPGTVTNVSPWMFTVGASSIDRGFFSYVYLGNKKQVKGSSLSSGGLPRGKLYPLMNSV 381
Query: 361 DAKAANVPDKLAQLCEKDSLDPAKAKGKIVVCLCGDDENVDQGFEVRRAGGVGLILVNDE 420
+AKA+N D LAQLCE+ SLDP KA+GKI+VCL GD+ +D+ FEV R GGVG+ILVND+
Sbjct: 382 NAKASNASDGLAQLCEEGSLDPMKARGKIIVCLRGDNGRMDKSFEVLRVGGVGMILVNDK 441
Query: 421 EDGADTISDPHFLPASHVSHEDGLLIAQYMKSTTTPEASISAVKTEIGVKPSPVMAVFSS 480
G+D +D H LP SHVS+ DGL IA+Y+KST P ASI+ V+TEIG+KPSPVMA FSS
Sbjct: 442 ISGSDIETDAHMLPTSHVSYIDGLSIAEYLKSTKRPVASITPVRTEIGIKPSPVMAPFSS 501
Query: 481 RGPNPIEESMIKPDITAPGVTIIASMPEAVAASKSALDTRRVPFNILSGTSMACPHISGI 540
RGPN I E+MIKPDI+APGV IIAS +AVAA+ D RRVPFN+ SGTSM+CPHI+G+
Sbjct: 502 RGPNHIAEAMIKPDISAPGVNIIASFTKAVAATDLPFDKRRVPFNVQSGTSMSCPHIAGV 561
Query: 541 AGLLRVLNPTWSPAAIRSAMMTTAITIDNTNHSMLNSNKADATPFDYGAGYVQPNNAMDP 600
AGLL+ L+PTWSPAAI+SA+MTTA T DNT ++ML+ NK ATPFDYGAG+V PN+AMDP
Sbjct: 562 AGLLKKLHPTWSPAAIKSAIMTTAKTRDNTKNTMLDFNKVKATPFDYGAGHVHPNDAMDP 621
Query: 601 GLIYNTTIEDYLNFLCAKKYTDAELKKFSTQAFLCEESFAVTDLNYPSISIPKLAAGAPV 660
GL+Y+TTI+DYLNFLC + Y LKKFS + F+C ++FA TDLNYPSIS+PKL G PV
Sbjct: 622 GLVYDTTIDDYLNFLCMQGYNSLTLKKFSNKPFVCAKNFASTDLNYPSISVPKLQIGVPV 681
Query: 661 TINRRLLNVGSPGTYVAQVKVPESVQAKVEPSSLQFSKVDEEKSFKIVFQHVGE-RRVSY 720
T+NRR+ NVGS GTYVA+V++P+ + VEPS+LQF V EEK+FK+VF + + RR Y
Sbjct: 682 TVNRRVKNVGSAGTYVARVRMPKGITVMVEPSTLQFHSVGEEKAFKLVFHYAQKVRRPGY 741
Query: 721 EFGTLEWSDGKHFVRSPLA 738
FG L WSDGKHFVRSP+A
Sbjct: 742 VFGALVWSDGKHFVRSPIA 757
BLAST of Sed0024525 vs. ExPASy TrEMBL
Match:
A0A5D3D732 (Subtilisin-like protease SBT5.3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold443G00170 PE=3 SV=1)
HSP 1 Score: 1010.4 bits (2611), Expect = 3.9e-291
Identity = 492/735 (66.94%), Postives = 594/735 (80.82%), Query Frame = 0
Query: 6 SYIVYLGGQKFGKKSSIFDVKLAKQSQFDILESVLESKVAVKESLIYSYSKYMNGFAALL 65
SYIVYLG FG SI DV+LA +SQ+++LESV+ SK+A KES+ YSY++Y+NGFAA+L
Sbjct: 743 SYIVYLGSHFFGSNPSIHDVQLATESQYELLESVVGSKLAAKESIFYSYNRYINGFAAIL 802
Query: 66 DEKQVVDLEKNPSVVSVFENTIRHLHTTRSWHFLGMENDAGIPANSIWNAAKFGEDVIIG 125
DE Q + L +NP+VVS+FEN R LHTTRSW FLGME+D GIP NSIW AA+FGED IIG
Sbjct: 803 DENQAIALARNPNVVSIFENQKRKLHTTRSWSFLGMESDEGIPPNSIWKAARFGEDTIIG 862
Query: 126 NLDTGVWPESKSFDDTGYGPVPKRWMGECDGGANFTCNRKLIGARYFYQGIESTNGPLNI 185
NLDTG WPESKSF+D GYGPVP RWMG C+GGANFTCN+KLIGARYF +G E+ NGP++
Sbjct: 863 NLDTGAWPESKSFNDAGYGPVPSRWMGVCEGGANFTCNKKLIGARYFNKGFEAENGPMSA 922
Query: 186 SMHTARDHEGHGSHTLSTAGGNLVPGANVFGSGNGTAKGGSPKARVVAYKVCWP---GGC 245
++ TARD EGHGSHTLSTAGGN VPGANVFG+GNGTAKGGSP+AR+ AYKVCWP GGC
Sbjct: 923 NLTTARDQEGHGSHTLSTAGGNFVPGANVFGNGNGTAKGGSPRARLAAYKVCWPSFTGGC 982
Query: 246 SDADILAGFEAAMDDGVDVLSVSLGSRATQFATDPVSVASFHAVQQGIVVVCSAGNNGPD 305
DADILA E+A+ DGVDVLS+SLGS A FA+D +S+ +FHAVQQGIVVVCS GN+GP
Sbjct: 983 YDADILAAVESAIHDGVDVLSISLGSSARDFASDTLSIGAFHAVQQGIVVVCSGGNDGPT 1042
Query: 306 PATVANIAPWMFTVAASTIDREFVSYVGLGNKKQIKGSSLSSEGLAVGSLHPLINAVDAK 365
P TV N++PWM TVAAST+DR+FV+YV LGNK+ KG SLSS GL G +PL++ V K
Sbjct: 1043 PGTVTNVSPWMITVAASTVDRDFVNYVALGNKRHFKGVSLSSGGLPRGKFYPLVDGVQVK 1102
Query: 366 AANVPDKLAQLCEKDSLDPAKAKGKIVVCLCGDDENVDQGFEVRRAGGVGLILVNDEEDG 425
A N DKLA LCE SLDPAKAKGKIV+CL GD +D+ FEVRRAGG+GLILVND+EDG
Sbjct: 1103 AGNATDKLALLCEDGSLDPAKAKGKIVLCLRGDSARMDKSFEVRRAGGIGLILVNDKEDG 1162
Query: 426 ADTISDPHFLPASHVSHEDGLLIAQYMKSTTTPEASISAVKTEIGVKPSPVMAVFSSRGP 485
D +DPHFLPASH+++ DG+ I QY+ ST +P A I+ VKTE+G+KPSP++A FSSRGP
Sbjct: 1163 NDITADPHFLPASHLNYADGIAIFQYINSTKSPMAFITHVKTELGIKPSPMVADFSSRGP 1222
Query: 486 NPIEESMIKPDITAPGVTIIASMPEAVAASKSALDTRRVPFNILSGTSMACPHISGIAGL 545
NPI +SMIKPDI APGV+I+A+ E A+ LDTRRV FN SGTSMACPHISG+ GL
Sbjct: 1223 NPIIDSMIKPDIAAPGVSILAAFSEYATATDFPLDTRRVSFNFESGTSMACPHISGVVGL 1282
Query: 546 LRVLNPTWSPAAIRSAMMTTAITIDNTNHSMLNSNKADATPFDYGAGYVQPNNAMDPGLI 605
L+ L P WSPAAI+SA+MTTA T DN+ S+L+ NKA ATPF YGAG+V PNNA+DPGL+
Sbjct: 1283 LKTLYPKWSPAAIKSAIMTTAKTRDNSMKSILDYNKAKATPFQYGAGHVHPNNAIDPGLV 1342
Query: 606 YNTTIEDYLNFLCAKKYTDAELKKFSTQAFLCEESFAVTDLNYPSISIPKLAAGAPVTIN 665
Y+TTIEDY+NF+CA+ Y LK+F + +LC +SF +TDLNYPSIS+PKL G PVTIN
Sbjct: 1343 YDTTIEDYMNFICAQGYNSTTLKRFYNKPYLCPKSFPLTDLNYPSISVPKLTIGVPVTIN 1402
Query: 666 RRLLNVGSPGTYVAQVKVPESVQAKVEPSSLQFSKVDEEKSFKIVFQHVGE-RRVSYEFG 725
RRL NVG+PGTYVA+VKV V V+PS+LQF+ V EEK+FK+VF++ G+ + + FG
Sbjct: 1403 RRLKNVGTPGTYVARVKVSSKVSVTVKPSTLQFNSVGEEKAFKVVFEYKGKGQGKGHVFG 1462
Query: 726 TLEWSDGKHFVRSPL 737
TL WSDGKHFVRSP+
Sbjct: 1463 TLIWSDGKHFVRSPM 1477
BLAST of Sed0024525 vs. ExPASy TrEMBL
Match:
A0A0A0L601 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G045060 PE=3 SV=1)
HSP 1 Score: 1010.0 bits (2610), Expect = 5.2e-291
Identity = 494/741 (66.67%), Postives = 596/741 (80.43%), Query Frame = 0
Query: 1 MHFEISYIVYLGGQKFGKKSSIFDVKLAKQSQFDILESVLESKVAVKESLIYSYSKYMNG 60
M +++SYIVYLG G + +D+++A +SQ+D+L SV+ SK+A K+++ YSY+KY+NG
Sbjct: 1 MEYDVSYIVYLGAPSVGSNPTNYDIEVATESQYDLLGSVVGSKLAAKDAIKYSYNKYING 60
Query: 61 FAALLDEKQVVDLEKNPSVVSVFENTIRHLHTTRSWHFLGMENDAGIPANSIWNAAKFGE 120
FAA LDEKQ DL KNP VVSVFEN R LHTTRSWHFLG+E+D GIP+NSIWNA +FGE
Sbjct: 61 FAATLDEKQAKDLAKNPKVVSVFENKERKLHTTRSWHFLGVESDEGIPSNSIWNAGRFGE 120
Query: 121 DVIIGNLDTGVWPESKSFDDTGYGPVPKRWMGECDGGANFTCNRKLIGARYFYQGIESTN 180
D IIGNLDTGVWPESKSF+D GYGPVP RW G C+GGANF CNRKLIGARYF +G +
Sbjct: 121 DTIIGNLDTGVWPESKSFNDAGYGPVPSRWRGACEGGANFRCNRKLIGARYFNKGFAMAS 180
Query: 181 GPLNISMHTARDHEGHGSHTLSTAGGNLVPGANVFGSGNGTAKGGSPKARVVAYKVCWP- 240
GPLNIS +TARD +GHGSHTLSTAGGN VPGANVFG GNGTAKGGSPKARV AYKVCWP
Sbjct: 181 GPLNISFNTARDKQGHGSHTLSTAGGNFVPGANVFGYGNGTAKGGSPKARVAAYKVCWPA 240
Query: 241 ---GGCSDADILAGFEAAMDDGVDVLSVSLGSRATQFATDPVSVASFHAVQQGIVVVCSA 300
GGC DADILAGFEAA+ DGVDVLSVSLGS+ +FA D +S+ +FHAVQQGIVVVCSA
Sbjct: 241 TSGGGCYDADILAGFEAAISDGVDVLSVSLGSKPEEFAYDSMSIGAFHAVQQGIVVVCSA 300
Query: 301 GNNGPDPATVANIAPWMFTVAASTIDREFVSYVGLGNKKQIKGSSLSSEGLAVGSLHPLI 360
GN+GP P TV+NI+PWMFTVAAS+IDR+F SY LGNKK KGSS+SS LA G +PLI
Sbjct: 301 GNDGPGPGTVSNISPWMFTVAASSIDRDFTSYASLGNKKHYKGSSISSSALAGGKFYPLI 360
Query: 361 NAVDAKAANVPDKLAQLCEKDSLDPAKAKGKIVVCLCGDDENVDQGFEVRRAGGVGLILV 420
NAVDAKAAN + LAQLC K SLDP KAKGKI+VCL G++ V++GF V +AGGVG+ILV
Sbjct: 361 NAVDAKAANASEILAQLCHKGSLDPTKAKGKIIVCLRGENARVEKGFVVLQAGGVGMILV 420
Query: 421 NDEEDGADTISDPHFLPASHVSHEDGLLIAQYMKSTTTPEASISAVKTEIGVKPSPVMAV 480
N + G+ T +D H LPA+H+S+ DGL +AQY+ ST TP A I+ V+T++G+KPSPVMA
Sbjct: 421 NGKNGGSGTTADAHILPATHLSYTDGLAVAQYINSTKTPVAHITPVQTQLGIKPSPVMAD 480
Query: 481 FSSRGPNPIEESMIKPDITAPGVTIIASMPEAVAASKSALDTRRVPFNILSGTSMACPHI 540
FSSRGPNPI E+M+KPDIT PG++I+AS+ V A+ DTRRVPFN+ SGTSM+CPHI
Sbjct: 481 FSSRGPNPITEAMLKPDITGPGMSILASVTTDVTATTFPFDTRRVPFNVESGTSMSCPHI 540
Query: 541 SGIAGLLRVLNPTWSPAAIRSAMMTTAITIDNTNHSMLNSNKADATPFDYGAGYVQPNNA 600
SG+ GLL+ L PTWSPAAI+SA+MTTA T DNT ++ ++ K ATPFDYGAG+V PN+A
Sbjct: 541 SGVVGLLKTLYPTWSPAAIKSAIMTTAKTRDNTMRTISDNVKPKATPFDYGAGHVHPNSA 600
Query: 601 MDPGLIYNTTIEDYLNFLCAKKYTDAELKKFSTQAFLCEESFAVTDLNYPSISIPKLAAG 660
MDPGL+Y+TTI+DYLNFLCA+ Y K F + F+C +SF +TDLNYPSISIPKL G
Sbjct: 601 MDPGLVYDTTIDDYLNFLCARGYNSLTFKNFYNKPFVCAKSFTLTDLNYPSISIPKLQFG 660
Query: 661 APVTINRRLLNVGSPGTYVAQVKVPESVQAKVEPSSLQFSKVDEEKSFKIVFQHVG-ERR 720
APVT+NRR+ NVG+PGTYVA+V + VEPS+LQF+ V EEK+FK+VF++ G E+
Sbjct: 661 APVTVNRRVKNVGTPGTYVARVNASSKILVTVEPSTLQFNSVGEEKAFKVVFEYKGNEQD 720
Query: 721 VSYEFGTLEWSDGKHFVRSPL 737
Y FGTL WSDGKH VRSP+
Sbjct: 721 KGYVFGTLIWSDGKHNVRSPI 741
BLAST of Sed0024525 vs. ExPASy TrEMBL
Match:
A0A1S3BLG8 (subtilisin-like protease SBT5.3 OS=Cucumis melo OX=3656 GN=LOC103491134 PE=3 SV=1)
HSP 1 Score: 1009.6 bits (2609), Expect = 6.7e-291
Identity = 492/736 (66.85%), Postives = 594/736 (80.71%), Query Frame = 0
Query: 6 SYIVYLGGQKFGKKSSIFDVKLAKQSQFDILESVLESKVAVKESLIYSYSKYMNGFAALL 65
SYIVYLG FG SI DV+LA +SQ+++LESV+ SK+A KES+ YSY++Y+NGFAA+L
Sbjct: 772 SYIVYLGSHFFGSNPSIHDVQLATESQYELLESVVGSKLAAKESIFYSYNRYINGFAAIL 831
Query: 66 DEKQVVDLEKNPSVVSVFENTIRHLHTTRSWHFLGMENDAGIPANSIWNAAKFGEDVIIG 125
DE Q + L +NP+VVS+FEN R LHTTRSW FLGME+D GIP NSIW AA+FGED IIG
Sbjct: 832 DENQAIALARNPNVVSIFENQKRKLHTTRSWSFLGMESDEGIPPNSIWKAARFGEDTIIG 891
Query: 126 NLDTGVWPESKSFDDTGYGPVPKRWMGECDGGANFTCNRKLIGARYFYQGIESTNGPLNI 185
NLDTG WPESKSF+D GYGPVP RWMG C+GGANFTCN+KLIGARYF +G E+ NGP++
Sbjct: 892 NLDTGAWPESKSFNDAGYGPVPSRWMGVCEGGANFTCNKKLIGARYFNKGFEAENGPMSA 951
Query: 186 SMHTARDHEGHGSHTLSTAGGNLVPGANVFGSGNGTAKGGSPKARVVAYKVCWP---GGC 245
++ TARD EGHGSHTLSTAGGN VPGANVFG+GNGTAKGGSP+AR+ AYKVCWP GGC
Sbjct: 952 NLTTARDQEGHGSHTLSTAGGNFVPGANVFGNGNGTAKGGSPRARLAAYKVCWPSFTGGC 1011
Query: 246 SDADILAGFEAAMDDGVDVLSVSLGSRATQFATDPVSVASFHAVQQGIVVVCSAGNNGPD 305
DADILA E+A+ DGVDVLS+SLGS A FA+D +S+ +FHAVQQGIVVVCS GN+GP
Sbjct: 1012 YDADILAAVESAIHDGVDVLSISLGSSARDFASDTLSIGAFHAVQQGIVVVCSGGNDGPT 1071
Query: 306 PATVANIAPWMFTVAASTIDREFVSYVGLGNKKQIKGSSLSSEGLAVGSLHPLINAVDAK 365
P TV N++PWM TVAAST+DR+FV+YV LGNK+ KG SLSS GL G +PL++ V K
Sbjct: 1072 PGTVTNVSPWMITVAASTVDRDFVNYVALGNKRHFKGVSLSSGGLPRGKFYPLVDGVQVK 1131
Query: 366 AANVPDKLAQLCEKDSLDPAKAKGKIVVCLCGDDENVDQGFEVRRAGGVGLILVNDEEDG 425
A N DKLA LCE SLDPAKAKGKIV+CL GD +D+ FEVRRAGG+GLILVND+EDG
Sbjct: 1132 AGNATDKLALLCEDGSLDPAKAKGKIVLCLRGDSARMDKSFEVRRAGGIGLILVNDKEDG 1191
Query: 426 ADTISDPHFLPASHVSHEDGLLIAQYMKSTTTPEASISAVKTEIGVKPSPVMAVFSSRGP 485
D +DPHFLPASH+++ DG+ I QY+ ST +P A I+ VKTE+G+KPSP++A FSSRGP
Sbjct: 1192 NDITADPHFLPASHLNYADGIAIFQYINSTKSPMAFITHVKTEMGIKPSPMVADFSSRGP 1251
Query: 486 NPIEESMIKPDITAPGVTIIASMPEAVAASKSALDTRRVPFNILSGTSMACPHISGIAGL 545
NPI +SMIKPDI APGV+I+A+ E A+ LDTRRV FN SGTSMACPHISG+ GL
Sbjct: 1252 NPIIDSMIKPDIAAPGVSILAAFSEYATATDFPLDTRRVSFNFESGTSMACPHISGVVGL 1311
Query: 546 LRVLNPTWSPAAIRSAMMTTAITIDNTNHSMLNSNKADATPFDYGAGYVQPNNAMDPGLI 605
L+ L P WSPAAI+SA+MTTA T DN+ S+L+ NKA ATPF YGAG+V PNNA+DPGL+
Sbjct: 1312 LKTLYPKWSPAAIKSAIMTTAKTRDNSMKSILDYNKAKATPFQYGAGHVHPNNAIDPGLV 1371
Query: 606 YNTTIEDYLNFLCAKKYTDAELKKFSTQAFLCEESFAVTDLNYPSISIPKLAAGAPVTIN 665
Y+TTIEDY+NF+CA+ Y LK+F + +LC +SF +TDLNYPSIS+PKL G PVTIN
Sbjct: 1372 YDTTIEDYMNFICAQGYNSTTLKRFYNKPYLCPKSFPLTDLNYPSISVPKLTIGVPVTIN 1431
Query: 666 RRLLNVGSPGTYVAQVKVPESVQAKVEPSSLQFSKVDEEKSFKIVFQHVGE-RRVSYEFG 725
RRL NVG+PGTYVA+VKV V V+PS+LQF+ V EEK+FK+VF++ G+ + + FG
Sbjct: 1432 RRLKNVGTPGTYVARVKVSSKVSVTVKPSTLQFNSVGEEKAFKVVFEYKGKGQGKGHVFG 1491
Query: 726 TLEWSDGKHFVRSPLA 738
TL WSDG HFVRSP+A
Sbjct: 1492 TLIWSDGNHFVRSPMA 1507
BLAST of Sed0024525 vs. TAIR 10
Match:
AT2G04160.1 (Subtilisin-like serine endopeptidase family protein )
HSP 1 Score: 735.7 bits (1898), Expect = 3.6e-212
Identity = 374/739 (50.61%), Postives = 503/739 (68.06%), Query Frame = 0
Query: 6 SYIVYLGGQKFGKKSSIFDVKLAKQSQFDILESVLESKVAVKESLIYSYSKYMNGFAALL 65
SY+VY G + + + K++ +D L S S+ +++ YSY+K++NGFAA L
Sbjct: 31 SYVVYFGAHSHVGEITEDAMDRVKETHYDFLGSFTGSRERATDAIFYSYTKHINGFAAHL 90
Query: 66 DEKQVVDLEKNPSVVSVFENTIRHLHTTRSWHFLGMENDAGIPANSIWNAAKFGEDVIIG 125
D ++ K+P VVSVF N LHTTRSW FLG+E+++ +P++SIW A+FGED II
Sbjct: 91 DHDLAYEISKHPEVVSVFPNKALKLHTTRSWDFLGLEHNSYVPSSSIWRKARFGEDTIIA 150
Query: 126 NLDTGVWPESKSFDDTGYGPVPKRWMGECDG--GANFTCNRKLIGARYFYQGIESTNGPL 185
NLDTGVWPESKSF D G GP+P RW G C A F CNRKLIGARYF +G + G L
Sbjct: 151 NLDTGVWPESKSFRDEGLGPIPSRWKGICQNQKDATFHCNRKLIGARYFNKGYAAAVGHL 210
Query: 186 NISMHTARDHEGHGSHTLSTAGGNLVPGANVFGSGNGTAKGGSPKARVVAYKVCWP---- 245
N S + RD +GHGSHTLSTA G+ VPG ++FG GNGTAKGGSP+ARV AYKVCWP
Sbjct: 211 NSSFDSPRDLDGHGSHTLSTAAGDFVPGVSIFGQGNGTAKGGSPRARVAAYKVCWPPVKG 270
Query: 246 GGCSDADILAGFEAAMDDGVDVLSVSLGSRATQFATDPVSVASFHAVQQGIVVVCSAGNN 305
C DAD+LA F+AA+ DG DV+SVSLG T F D V++ SFHA ++ IVVVCSAGN+
Sbjct: 271 NECYDADVLAAFDAAIHDGADVISVSLGGEPTSFFNDSVAIGSFHAAKKRIVVVCSAGNS 330
Query: 306 GPDPATVANIAPWMFTVAASTIDREFVSYVGLGNKKQIKGSSLSSEGLAVGSLHPLINAV 365
GP +TV+N+APW TV AST+DREF S + LGN K KG SLSS L +P++ +V
Sbjct: 331 GPADSTVSNVAPWQITVGASTMDREFASNLVLGNGKHYKGQSLSSTALPHAKFYPIMASV 390
Query: 366 DAKAANVPDKLAQLCEKDSLDPAKAKGKIVVCLCGDDENVDQGFEVRRAGGVGLILVNDE 425
+AKA N AQLC+ SLDP K KGKI+VCL G + V++G V GG+G++L N
Sbjct: 391 NAKAKNASALDAQLCKLGSLDPIKTKGKILVCLRGQNGRVEKGRAVALGGGIGMVLENTY 450
Query: 426 EDGADTISDPHFLPASHVSHEDGLLIAQYMKSTTTPEASISAVKTEIGVKPSPVMAVFSS 485
G D ++DPH LPA+ ++ +D +++Y+ T P A I+ +T++G+KP+PVMA FSS
Sbjct: 451 VTGNDLLADPHVLPATQLTSKDSFAVSRYISQTKKPIAHITPSRTDLGLKPAPVMASFSS 510
Query: 486 RGPNPIEESMIKPDITAPGVTIIASMPEAVAASKSALDTRRVPFNILSGTSMACPHISGI 545
+GP+ + ++KPDITAPGV++IA+ AV+ + D RR+ FN +SGTSM+CPHISGI
Sbjct: 511 KGPSIVAPQILKPDITAPGVSVIAAYTGAVSPTNEQFDPRRLLFNAISGTSMSCPHISGI 570
Query: 546 AGLLRVLNPTWSPAAIRSAMMTTAITIDNTNHSMLNSNKADATPFDYGAGYVQPNNAMDP 605
AGLL+ P+WSPAAIRSA+MTTA +D+ + N+ ATPF +GAG+VQPN A++P
Sbjct: 571 AGLLKTRYPSWSPAAIRSAIMTTATIMDDIPGPIQNATNMKATPFSFGAGHVQPNLAVNP 630
Query: 606 GLIYNTTIEDYLNFLCAKKYTDAELKKFSTQAFLCEE-SFAVTDLNYPSISIPKLAAGAP 665
GL+Y+ I+DYLNFLC+ Y +++ FS F C ++ +LNYPSI++P L + +
Sbjct: 631 GLVYDLGIKDYLNFLCSLGYNASQISVFSGNNFTCSSPKISLVNLNYPSITVPNLTS-SK 690
Query: 666 VTINRRLLNVGSPGTYVAQVKVPESVQAKVEPSSLQFSKVDEEKSFKIVF-QHVGERRVS 725
VT++R + NVG P Y +V P+ V V+P+SL F+KV E+K+FK++ + G
Sbjct: 691 VTVSRTVKNVGRPSMYTVKVNNPQGVYVAVKPTSLNFTKVGEQKTFKVILVKSKGNVAKG 750
Query: 726 YEFGTLEWSDGKHFVRSPL 737
Y FG L WSD KH VRSP+
Sbjct: 751 YVFGELVWSDKKHRVRSPI 768
BLAST of Sed0024525 vs. TAIR 10
Match:
AT5G59810.1 (Subtilase family protein )
HSP 1 Score: 716.5 bits (1848), Expect = 2.3e-206
Identity = 373/737 (50.61%), Postives = 481/737 (65.26%), Query Frame = 0
Query: 6 SYIVYLGGQKFGKKSSIFDVKLAKQSQFDILESVLESKVAVKESLIYSYSKYMNGFAALL 65
SYIVYLG + S + S L S + S KE++ YSY +++NGFAA+L
Sbjct: 41 SYIVYLGSHAHLPQISSAHLDGVAHSHRTFLASFVGSHENAKEAIFYSYKRHINGFAAIL 100
Query: 66 DEKQVVDLEKNPSVVSVFENTIRHLHTTRSWHFLGMENDAGIPANSIWNAAKFGEDVIIG 125
DE + ++ K+P VVSVF N R LHTT SW+F+ + + + +S+WN A +GED II
Sbjct: 101 DENEAAEIAKHPDVVSVFPNKGRKLHTTHSWNFMLLAKNGVVHKSSLWNKAGYGEDTIIA 160
Query: 126 NLDTGVWPESKSFDDTGYGPVPKRWMGECDGGANFTCNRKLIGARYFYQGIESTNG-PLN 185
NLDTGVWPESKSF D GYG VP RW G C + CNRKLIGARYF +G + G P N
Sbjct: 161 NLDTGVWPESKSFSDEGYGAVPARWKGRCH--KDVPCNRKLIGARYFNKGYLAYTGLPSN 220
Query: 186 ISMHTARDHEGHGSHTLSTAGGNLVPGANVFGSGNGTAKGGSPKARVVAYKVCWP----G 245
S T RDH+GHGSHTLSTA GN VPGANVFG GNGTA GGSPKARV AYKVCWP
Sbjct: 221 ASYETCRDHDGHGSHTLSTAAGNFVPGANVFGIGNGTASGGSPKARVAAYKVCWPPVDGA 280
Query: 246 GCSDADILAGFEAAMDDGVDVLSVSLGSRATQFATDPVSVASFHAVQQGIVVVCSAGNNG 305
C DADILA EAA++DGVDVLS S+G A + +D +++ SFHAV+ G+ VVCSAGN+G
Sbjct: 281 ECFDADILAAIEAAIEDGVDVLSASVGGDAGDYMSDGIAIGSFHAVKNGVTVVCSAGNSG 340
Query: 306 PDPATVANIAPWMFTVAASTIDREFVSYVGLGNKKQIKGSSLSSEGLAVGSLHPLINAVD 365
P TV+N+APW+ TV AS++DREF ++V L N + KG+SL S+ L ++ LI+A D
Sbjct: 341 PKSGTVSNVAPWVITVGASSMDREFQAFVELKNGQSFKGTSL-SKPLPEEKMYSLISAAD 400
Query: 366 AKAANVPDKLAQLCEKDSLDPAKAKGKIVVCLCGDDENVDQGFEVRRAGGVGLILVNDEE 425
A AN A LC+K SLDP K KGKI+VCL GD+ VD+G + AG G++L ND+
Sbjct: 401 ANVANGNVTDALLCKKGSLDPKKVKGKILVCLRGDNARVDKGMQAAAAGAAGMVLCNDKA 460
Query: 426 DGADTISDPHFLPASHVSHEDGLLIAQYMKSTTTPEASISAVKTEIGVKPSPVMAVFSSR 485
G + ISD H LPAS + ++DG + Y+ ST P+ I A + KP+P MA FSSR
Sbjct: 461 SGNEIISDAHVLPASQIDYKDGETLFSYLSSTKDPKGYIKAPTATLNTKPAPFMASFSSR 520
Query: 486 GPNPIEESMIKPDITAPGVTIIASMPEAVAASKSALDTRRVPFNILSGTSMACPHISGIA 545
GPN I ++KPDITAPGV IIA+ EA + D RR PFN SGTSM+CPHISG+
Sbjct: 521 GPNTITPGILKPDITAPGVNIIAAFTEATGPTDLDSDNRRTPFNTESGTSMSCPHISGVV 580
Query: 546 GLLRVLNPTWSPAAIRSAMMTTAITIDNTNHSMLNSNKADATPFDYGAGYVQPNNAMDPG 605
GLL+ L+P WSPAAIRSA+MTT+ T +N M++ + A PF YG+G+VQPN A PG
Sbjct: 581 GLLKTLHPHWSPAAIRSAIMTTSRTRNNRRKPMVDESFKKANPFSYGSGHVQPNKAAHPG 640
Query: 606 LIYNTTIEDYLNFLCAKKYTDAELKKFSTQ-AFLCEESFAVTDLNYPSISIPKLAAGAPV 665
L+Y+ T DYL+FLCA Y + ++ F+ + C + + D NYPSI++P L +
Sbjct: 641 LVYDLTTGDYLDFLCAVGYNNTVVQLFAEDPQYTCRQGANLLDFNYPSITVPNLTGS--I 700
Query: 666 TINRRLLNVGSPGTYVAQVKVPESVQAKVEPSSLQFSKVDEEKSFKIVFQHVGERRVSYE 725
T+ R+L NVG P TY A+ + P V+ VEP L F+K E K F++ + + Y
Sbjct: 701 TVTRKLKNVGPPATYNARFREPLGVRVSVEPKQLTFNKTGEVKIFQMTLRPLPVTPSGYV 760
Query: 726 FGTLEWSDGKHFVRSPL 737
FG L W+D H+VRSP+
Sbjct: 761 FGELTWTDSHHYVRSPI 772
BLAST of Sed0024525 vs. TAIR 10
Match:
AT5G67360.1 (Subtilase family protein )
HSP 1 Score: 579.7 bits (1493), Expect = 3.3e-165
Identity = 319/720 (44.31%), Postives = 450/720 (62.50%), Query Frame = 0
Query: 31 SQFDILESVLES---KVAVKESLIYSYSKYMNGFAALLDEKQVVDLEKNPSVVSVFENTI 90
S FD+ + +S ++ L+Y+Y ++GF+ L +++ L P V+SV
Sbjct: 43 SSFDLHSNWYDSSLRSISDSAELLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHR 102
Query: 91 RHLHTTRSWHFLGMENDAGIPANSIWNAAKFGEDVIIGNLDTGVWPESKSFDDTGYGPVP 150
LHTTR+ FLG++ ++ A DV++G LDTGVWPESKS+ D G+GP+P
Sbjct: 103 YELHTTRTPLFLGLDEHTA----DLFPEAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIP 162
Query: 151 KRWMGECDGGANFT---CNRKLIGARYFYQGIESTNGPLNIS--MHTARDHEGHGSHTLS 210
W G C+ G NFT CNRKLIGAR+F +G EST GP++ S + RD +GHG+HT S
Sbjct: 163 SSWKGGCEAGTNFTASLCNRKLIGARFFARGYESTMGPIDESKESRSPRDDDGHGTHTSS 222
Query: 211 TAGGNLVPGANVFGSGNGTAKGGSPKARVVAYKVCWPGGCSDADILAGFEAAMDDGVDVL 270
TA G++V GA++ G +GTA+G +P+ARV YKVCW GGC +DILA + A+ D V+VL
Sbjct: 223 TAAGSVVEGASLLGYASGTARGMAPRARVAVYKVCWLGGCFSSDILAAIDKAIADNVNVL 282
Query: 271 SVSLGSRATQFATDPVSVASFHAVQQGIVVVCSAGNNGPDPATVANIAPWMFTVAASTID 330
S+SLG + + D V++ +F A+++GI+V CSAGN GP ++++N+APW+ TV A T+D
Sbjct: 283 SMSLGGGMSDYYRDGVAIGAFAAMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLD 342
Query: 331 REFVSYVGLGNKKQIKGSSLSSEGLAVGSLHPLINAVDAKAANVPDKLAQLCEKDSLDPA 390
R+F + LGN K G SL L P I A +A A LC +L P
Sbjct: 343 RDFPALAILGNGKNFTGVSLFKGEALPDKLLPFIYAGNASNAT----NGNLCMTGTLIPE 402
Query: 391 KAKGKIVVCLCGDDENVDQGFEVRRAGGVGLILVNDEEDGADTISDPHFLPASHVSHEDG 450
K KGKIV+C G + V +G V+ AGGVG+IL N +G + ++D H LPA+ V + G
Sbjct: 403 KVKGKIVMCDRGINARVQKGDVVKAAGGVGMILANTAANGEELVADAHLLPATTVGEKAG 462
Query: 451 LLIAQYMKSTTTPEASISAVKTEIGVKPSPVMAVFSSRGPNPIEESMIKPDITAPGVTII 510
+I Y+ + P ASIS + T +GVKPSPV+A FSSRGPN I +++KPD+ APGV I+
Sbjct: 463 DIIRHYVTTDPNPTASISILGTVVGVKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNIL 522
Query: 511 ASMPEAVAASKSALDTRRVPFNILSGTSMACPHISGIAGLLRVLNPTWSPAAIRSAMMTT 570
A+ A + A D+RRV FNI+SGTSM+CPH+SG+A LL+ ++P WSPAAIRSA+MTT
Sbjct: 523 AAWTGAAGPTGLASDSRRVEFNIISGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTT 582
Query: 571 AITIDNTNHSMLN-SNKADATPFDYGAGYVQPNNAMDPGLIYNTTIEDYLNFLCAKKYTD 630
A +L+ + +TPFD+GAG+V P A +PGLIY+ T EDYL FLCA YT
Sbjct: 583 AYKTYKDGKPLLDIATGKPSTPFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTS 642
Query: 631 AELKKFSTQAFLCE--ESFAVTDLNYPSISIPKLAAGAPVTINRRLLNVGSPGTYVAQV- 690
+++ S + + C+ +S++V DLNYPS ++ GA R + +VG GTY +V
Sbjct: 643 PQIRSVSRRNYTCDPSKSYSVADLNYPSFAVNVDGVGA-YKYTRTVTSVGGAGTYSVKVT 702
Query: 691 KVPESVQAKVEPSSLQFSKVDEEKSFKIVFQ-HVGERRVSYEFGTLEWSDGKHFVRSPLA 738
V+ VEP+ L F + +E+KS+ + F + S FG++EWSDGKH V SP+A
Sbjct: 703 SETTGVKISVEPAVLNFKEANEKKSYTVTFTVDSSKPSGSNSFGSIEWSDGKHVVGSPVA 753
BLAST of Sed0024525 vs. TAIR 10
Match:
AT3G14240.1 (Subtilase family protein )
HSP 1 Score: 539.7 bits (1389), Expect = 3.8e-153
Identity = 314/758 (41.42%), Postives = 439/758 (57.92%), Query Frame = 0
Query: 5 ISYIVYLGGQKFGKKSSIFDVKLAKQSQFDILESVLESKVAVKESLIYSYSKYMNGFAAL 64
++YIV++ + K SIF + F S L S + S+I++Y +GF+A
Sbjct: 26 LTYIVHVDHE---AKPSIF------PTHFHWYTSSLASLTSSPPSIIHTYDTVFHGFSAR 85
Query: 65 LDEKQVVDLEKNPSVVSVFENTIRHLHTTRSWHFLGME--NDAGIPANSIWNAAKFGEDV 124
L + L +P V+SV +RHLHTTRS FLG+ + AG+ S FG D+
Sbjct: 86 LTSQDASQLLDHPHVISVIPEQVRHLHTTRSPEFLGLRSTDKAGLLEES-----DFGSDL 145
Query: 125 IIGNLDTGVWPESKSFDDTGYGPVPKRWMGECDGGANF---TCNRKLIGARYFYQGIEST 184
+IG +DTGVWPE SFDD G GPVP +W G+C +F CNRKL+GAR+F G E+T
Sbjct: 146 VIGVIDTGVWPERPSFDDRGLGPVPIKWKGQCIASQDFPESACNRKLVGARFFCGGYEAT 205
Query: 185 NGPLN--ISMHTARDHEGHGSHTLSTAGGNLVPGANVFGSGNGTAKGGSPKARVVAYKVC 244
NG +N + RD +GHG+HT S + G V A+ G +G A G +PKAR+ AYKVC
Sbjct: 206 NGKMNETTEFRSPRDSDGHGTHTASISAGRYVFPASTLGYAHGVAAGMAPKARLAAYKVC 265
Query: 245 WPGGCSDADILAGFEAAMDDGVDVLSVSLGSRATQFATDPVSVASFHAVQQGIVVVCSAG 304
W GC D+DILA F+ A+ DGVDV+S+S+G + D +++ +F A+ +GI V SAG
Sbjct: 266 WNSGCYDSDILAAFDTAVADGVDVISLSVGGVVVPYYLDAIAIGAFGAIDRGIFVSASAG 325
Query: 305 NNGPDPATVANIAPWMFTVAASTIDREFVSYVGLGNKKQIKGSSL-SSEGLAVGSLHPLI 364
N GP TV N+APWM TV A TIDR+F + V LGN K I G S+ GL G ++PL+
Sbjct: 326 NGGPGALTVTNVAPWMTTVGAGTIDRDFPANVKLGNGKMISGVSVYGGPGLDPGRMYPLV 385
Query: 365 NAVDAKAANVPDKLAQLCEKDSLDPAKAKGKIVVCLCGDDENVDQGFEVRRAGGVGLILV 424
+ + LC + SLDP KGKIV+C G + +G VR+ GG+G+I+
Sbjct: 386 YGGSLLGGD--GYSSSLCLEGSLDPNLVKGKIVLCDRGINSRATKGEIVRKNGGLGMIIA 445
Query: 425 NDEEDGADTISDPHFLPASHVSHEDGLLIAQYM------KSTTTPEASISAVKTEIGVKP 484
N DG ++D H LPA+ V G I +Y+ +S+ P A+I T +G++P
Sbjct: 446 NGVFDGEGLVADCHVLPATSVGASGGDEIRRYISESSKSRSSKHPTATIVFKGTRLGIRP 505
Query: 485 SPVMAVFSSRGPNPIEESMIKPDITAPGVTIIASMPEAVAASKSALDTRRVPFNILSGTS 544
+PV+A FS+RGPNP ++KPD+ APG+ I+A+ P+ + S D RR FNILSGTS
Sbjct: 506 APVVASFSARGPNPETPEILKPDVIAPGLNILAAWPDRIGPSGVTSDNRRTEFNILSGTS 565
Query: 545 MACPHISGIAGLLRVLNPTWSPAAIRSAMMTTAITIDNTNHSMLNSNKAD-ATPFDYGAG 604
MACPH+SG+A LL+ +P WSPAAIRSA++TTA T+DN+ M++ + + ++ DYG+G
Sbjct: 566 MACPHVSGLAALLKAAHPDWSPAAIRSALITTAYTVDNSGEPMMDESTGNTSSVMDYGSG 625
Query: 605 YVQPNNAMDPGLIYNTTIEDYLNFLCAKKYTDAELKKFSTQAFLCE---ESFAVTDLNYP 664
+V P AMDPGL+Y+ T DY+NFLC YT + + + C+ + V +LNYP
Sbjct: 626 HVHPTKAMDPGLVYDITSYDYINFLCNSNYTRTNIVTITRRQADCDGARRAGHVGNLNYP 685
Query: 665 SISIPKLAAG---APVTINRRLLNVG-SPGTYVAQVKVPESVQAKVEPSSLQFSKVDEEK 724
S S+ G R + NVG S Y +++ P VEP L F +V ++
Sbjct: 686 SFSVVFQQYGESKMSTHFIRTVTNVGDSDSVYEIKIRPPRGTTVTVEPEKLSFRRVGQKL 745
Query: 725 SF----KIVFQHVGERRVSYEFGTLEWSDGKHFVRSPL 737
SF K + + E G + WSDGK V SPL
Sbjct: 746 SFVVRVKTTEVKLSPGATNVETGHIVWSDGKRNVTSPL 767
BLAST of Sed0024525 vs. TAIR 10
Match:
AT3G14067.1 (Subtilase family protein )
HSP 1 Score: 539.3 bits (1388), Expect = 5.0e-153
Identity = 307/712 (43.12%), Postives = 439/712 (61.66%), Query Frame = 0
Query: 49 SLIYSYSKYMNGFAALLDEKQVVDLEKNPSVVSVFENTIRHLHTTRSWHFLGMENDAGIP 108
+L+YSYS+ ++GF+A L Q L ++PSV+SV + R +HTT + FLG ++G
Sbjct: 68 TLLYSYSRAVHGFSARLSPIQTAALRRHPSVISVIPDQAREIHTTHTPAFLGFSQNSG-- 127
Query: 109 ANSIWNAAKFGEDVIIGNLDTGVWPESKSFDDTGYGPVPKRWMGECDGGANF---TCNRK 168
+W+ + +GEDVI+G LDTG+WPE SF D+G GP+P W GEC+ G +F +CNRK
Sbjct: 128 ---LWSNSNYGEDVIVGVLDTGIWPEHPSFSDSGLGPIPSTWKGECEIGPDFPASSCNRK 187
Query: 169 LIGARYFYQG-IESTNGPLNIS---MHTARDHEGHGSHTLSTAGGNLVPGANVFGSGNGT 228
LIGAR FY+G + NG + + RD EGHG+HT STA G++V A+++ GT
Sbjct: 188 LIGARAFYRGYLTQRNGTKKHAAKESRSPRDTEGHGTHTASTAAGSVVANASLYQYARGT 247
Query: 229 AKGGSPKARVVAYKVCWPGGCSDADILAGFEAAMDDGVDVLSVSLGS--RATQFATDPVS 288
A G + KAR+ AYK+CW GGC D+DILA + A+ DGV V+S+S+G+ A ++ TD ++
Sbjct: 248 ATGMASKARIAAYKICWTGGCYDSDILAAMDQAVADGVHVISLSVGASGSAPEYHTDSIA 307
Query: 289 VASFHAVQQGIVVVCSAGNNGPDPATVANIAPWMFTVAASTIDREFVSYVGLGNKKQIKG 348
+ +F A + GIVV CSAGN+GP+P T NIAPW+ TV AST+DREF + G+ K G
Sbjct: 308 IGAFGATRHGIVVSCSAGNSGPNPETATNIAPWILTVGASTVDREFAANAITGDGKVFTG 367
Query: 349 SSLSSEGLAVGSLHPLINAVDAKAANVPDKLAQLCEKDSLDPAKAKGKIVVCLCGDDENV 408
+SL + S L+ + D ++LC L+ + +GKIV+C G + V
Sbjct: 368 TSLYAGESLPDSQLSLVYSGDCG--------SRLCYPGKLNSSLVEGKIVLCDRGGNARV 427
Query: 409 DQGFEVRRAGGVGLILVNDEEDGADTISDPHFLPASHVSHEDGLLIAQYMKSTTTPEASI 468
++G V+ AGG G+IL N E G + +D H +PA+ V + G I Y+K++ +P A I
Sbjct: 428 EKGSAVKLAGGAGMILANTAESGEELTADSHLVPATMVGAKAGDQIRDYIKTSDSPTAKI 487
Query: 469 SAVKTEIGVK-PSPVMAVFSSRGPNPIEESMIKPDITAPGVTIIASMPEAVAASKSALDT 528
S + T IG PSP +A FSSRGPN + ++KPD+ APGV I+A V + +D
Sbjct: 488 SFLGTLIGPSPPSPRVAAFSSRGPNHLTPVILKPDVIAPGVNILAGWTGMVGPTDLDIDP 547
Query: 529 RRVPFNILSGTSMACPHISGIAGLLRVLNPTWSPAAIRSAMMTTAITIDNTNHSMLN-SN 588
RRV FNI+SGTSM+CPH+SG+A LLR +P WSPAAI+SA++TTA ++N+ + + +
Sbjct: 548 RRVQFNIISGTSMSCPHVSGLAALLRKAHPDWSPAAIKSALVTTAYDVENSGEPIEDLAT 607
Query: 589 KADATPFDYGAGYVQPNNAMDPGLIYNTTIEDYLNFLCAKKYTDAELKKFSTQAFL---C 648
+ F +GAG+V PN A++PGL+Y+ +++Y+ FLCA Y + F L C
Sbjct: 608 GKSSNSFIHGAGHVDPNKALNPGLVYDIEVKEYVAFLCAVGYEFPGILVFLQDPTLYDAC 667
Query: 649 EESFAVT--DLNYPSISIPKLAAGAPVTINRRLLNVGS--PGTYVAQVKVPESVQAKVEP 708
E S T DLNYPS S+ + G V R + NVGS Y VK P +V+ V P
Sbjct: 668 ETSKLRTAGDLNYPSFSVVFASTGEVVKYKRVVKNVGSNVDAVYEVGVKSPANVEIDVSP 727
Query: 709 SSLQFSK----VDEEKSFKIVFQHVGERRV-SYEFGTLEWSDGKHFVRSPLA 738
S L FSK ++ E +FK V G V +EFG++EW+DG+H V+SP+A
Sbjct: 728 SKLAFSKEKSVLEYEVTFKSVVLGGGVGSVPGHEFGSIEWTDGEHVVKSPVA 766
The following BLAST results are available for this feature:
| Match Name | E-value | Identity | Description | |
|---|
| XP_038905686.1 | 1.2e-297 | 68.89 | subtilisin-like protease SBT5.3 [Benincasa hispida] | [more] |
| XP_022949775.1 | 3.0e-293 | 68.02 | subtilisin-like protease SBT5.3 [Cucurbita moschata] | [more] |
| XP_023005962.1 | 2.5e-292 | 67.93 | uncharacterized protein LOC111498820 [Cucurbita maxima] | [more] |
| XP_038905648.1 | 3.3e-292 | 67.93 | subtilisin-like protease SBT5.4 [Benincasa hispida] | [more] |
| KAG6596783.1 | 5.6e-292 | 67.88 | Subtilisin-like protease 5.3, partial [Cucurbita argyrosperma subsp. sororia] | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| Q9ZSP5 | 5.1e-211 | 50.61 | Subtilisin-like protease SBT5.3 OS=Arabidopsis thaliana OX=3702 GN=AIR3 PE=2 SV=... | [more] |
| F4JXC5 | 3.2e-205 | 50.61 | Subtilisin-like protease SBT5.4 OS=Arabidopsis thaliana OX=3702 GN=SBT5.4 PE=1 S... | [more] |
| I1N462 | 9.0e-192 | 48.09 | Subtilisin-like protease Glyma18g48580 OS=Glycine max OX=3847 GN=Glyma18g48580 P... | [more] |
| O65351 | 4.7e-164 | 44.31 | Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 S... | [more] |
| Q9LUM3 | 5.3e-152 | 41.42 | Subtilisin-like protease SBT1.5 OS=Arabidopsis thaliana OX=3702 GN=SBT1.5 PE=2 S... | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| A0A6J1GDR2 | 1.4e-293 | 68.02 | subtilisin-like protease SBT5.3 OS=Cucurbita moschata OX=3662 GN=LOC111453063 PE... | [more] |
| A0A6J1KUL8 | 1.2e-292 | 67.93 | uncharacterized protein LOC111498820 OS=Cucurbita maxima OX=3661 GN=LOC111498820... | [more] |
| A0A5D3D732 | 3.9e-291 | 66.94 | Subtilisin-like protease SBT5.3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_... | [more] |
| A0A0A0L601 | 5.2e-291 | 66.67 | Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G045060 PE=3 SV=1 | [more] |
| A0A1S3BLG8 | 6.7e-291 | 66.85 | subtilisin-like protease SBT5.3 OS=Cucumis melo OX=3656 GN=LOC103491134 PE=3 SV=... | [more] |
