Homology
BLAST of Sed0020249 vs. NCBI nr
Match:
CAA70330.1 (dioxygenase [Marah macrocarpa])
HSP 1 Score: 582.8 bits (1501), Expect = 1.8e-162
Identity = 294/326 (90.18%), Postives = 304/326 (93.25%), Query Frame = 0
Query: 1 MVALSKPAIQKLSSSSSSSSSSRVPLIDLSAPDAKHLIVKACEELGFFKVVNHGVPMEFI 60
MV LSKP IQ+LSSSSSS VPLIDLSAPDAKHLIVKACEELGFFKV+NHGVPMEFI
Sbjct: 1 MVVLSKPEIQQLSSSSSSG----VPLIDLSAPDAKHLIVKACEELGFFKVINHGVPMEFI 60
Query: 61 SALESESTKFFSLPLSEKHRAGPASPFGYGNKQIGRNGDVGWVEYLLLQTNSHGFLSTFA 120
S LESESTKFFSLPLSEK RAGP SPFGYGNKQIGRNGDVGWVEYLLLQ+NSH FLS F
Sbjct: 61 STLESESTKFFSLPLSEKQRAGPPSPFGYGNKQIGRNGDVGWVEYLLLQSNSHAFLSIFG 120
Query: 121 QHPQKLRSALNNYISSVRNMACKTLELMAEGLKIQQRNAFSKLLMDEESDSVFRVNHYPP 180
Q PQKLRSALN+YI +VRNMAC+ +ELMAEGLKIQQRNA SKLLM EESDSVFRVNHYPP
Sbjct: 121 QDPQKLRSALNDYIWAVRNMACEIVELMAEGLKIQQRNALSKLLMGEESDSVFRVNHYPP 180
Query: 181 CPEEVRALEGKNMIGFGEHTDPQIISVLRSNNTSGLQISLADGNWVSVPPDQNSFFINVG 240
CPEE++ALEG NMIGFGEHTDPQIISVLRSNNTSGLQISL D NW+SVPPDQ SFFINVG
Sbjct: 181 CPEELQALEGTNMIGFGEHTDPQIISVLRSNNTSGLQISLPDANWISVPPDQTSFFINVG 240
Query: 241 DSLQVMTNGRFKSVKHRVLTNSLKSRISMIYFGGPPLSEKIAPLPSLMKGEESLYKEFTW 300
DSLQVMTNGRFKSVKHRVLTNSLKSRISMIYFGGPPLSEKIAPLPSLMKGEESLYKEFTW
Sbjct: 241 DSLQVMTNGRFKSVKHRVLTNSLKSRISMIYFGGPPLSEKIAPLPSLMKGEESLYKEFTW 300
Query: 301 FEYKRSAYNSRLADNRLVHFERIAAS 327
FEYKRSAYNSRLADNRLVHFERIAAS
Sbjct: 301 FEYKRSAYNSRLADNRLVHFERIAAS 322
BLAST of Sed0020249 vs. NCBI nr
Match:
XP_023531222.1 (gibberellin 2-beta-dioxygenase 1-like [Cucurbita pepo subsp. pepo])
HSP 1 Score: 571.2 bits (1471), Expect = 5.6e-159
Identity = 294/341 (86.22%), Postives = 310/341 (90.91%), Query Frame = 0
Query: 1 MVALSKPAIQKLSS-----------SSSSSSSSRVPLIDLSAPDAKHLIVKACEELGFFK 60
MV LSKPAI++++S SSS++ S VPLIDLSAPDAKHLIVKACEELGFFK
Sbjct: 1 MVVLSKPAIKQMASSYMRNSMAAAGSSSAAFFSGVPLIDLSAPDAKHLIVKACEELGFFK 60
Query: 61 VVNHGVPMEFISALESESTKFFSLPLSEKHRAGPASPFGYGNKQIGRNGDVGWVEYLLLQ 120
VVNHGVPMEFIS LESESTKFFSLPLSEK RAGP SPFGYGNKQIGRNGDVGWVEYLLL
Sbjct: 61 VVNHGVPMEFISTLESESTKFFSLPLSEKQRAGPPSPFGYGNKQIGRNGDVGWVEYLLLN 120
Query: 121 T----NSHGFLSTFAQHPQKLRSALNNYISSVRNMACKTLELMAEGLKIQQRNAFSKLLM 180
T NS GFLS F Q PQKLRSA+N+YIS+VRNMAC+ LELMAEGLKIQQRNAFSKLLM
Sbjct: 121 THLESNSDGFLSIFGQDPQKLRSAVNDYISAVRNMACEILELMAEGLKIQQRNAFSKLLM 180
Query: 181 DEESDSVFRVNHYPPCPEEVRALEGKNMIGFGEHTDPQIISVLRSNNTSGLQISLADGNW 240
DEESDSVFRVNHYPPCP E++AL+G NMIGFGEHTDPQIISVLRSNNTSGLQISLADGNW
Sbjct: 181 DEESDSVFRVNHYPPCP-ELQALKGTNMIGFGEHTDPQIISVLRSNNTSGLQISLADGNW 240
Query: 241 VSVPPDQNSFFINVGDSLQVMTNGRFKSVKHRVLTNSLKSRISMIYFGGPPLSEKIAPLP 300
+SVPPDQ+SFFINVGDSLQVMTNGRFKSVKHRVLTN LKSR+SMIYFGGPPLSEKIAPLP
Sbjct: 241 ISVPPDQSSFFINVGDSLQVMTNGRFKSVKHRVLTNRLKSRVSMIYFGGPPLSEKIAPLP 300
Query: 301 SLMKGEESLYKEFTWFEYKRSAYNSRLADNRLVHFERIAAS 327
SLMKGEESLYKEFTWFEYKRSAYNSRLADNRLVHFERIAAS
Sbjct: 301 SLMKGEESLYKEFTWFEYKRSAYNSRLADNRLVHFERIAAS 340
BLAST of Sed0020249 vs. NCBI nr
Match:
KAG6600483.1 (Gibberellin 2-beta-dioxygenase 1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7031125.1 Gibberellin 2-beta-dioxygenase 1 [Cucurbita argyrosperma subsp. argyrosperma])
HSP 1 Score: 569.7 bits (1467), Expect = 1.6e-158
Identity = 293/341 (85.92%), Postives = 309/341 (90.62%), Query Frame = 0
Query: 1 MVALSKPAIQKLSS-----------SSSSSSSSRVPLIDLSAPDAKHLIVKACEELGFFK 60
MV LSKPAI++++S SSS++ S VPLIDLSAPDAKHLIVKACEELGFFK
Sbjct: 1 MVVLSKPAIKQMASSYMRNSMAAAGSSSAAFFSGVPLIDLSAPDAKHLIVKACEELGFFK 60
Query: 61 VVNHGVPMEFISALESESTKFFSLPLSEKHRAGPASPFGYGNKQIGRNGDVGWVEYLLLQ 120
VVNHGVPMEFIS LESESTKFFSLPLSEK RAGP SPFGYGNKQIGRNGDVGWVEYLLL
Sbjct: 61 VVNHGVPMEFISTLESESTKFFSLPLSEKQRAGPPSPFGYGNKQIGRNGDVGWVEYLLLN 120
Query: 121 T----NSHGFLSTFAQHPQKLRSALNNYISSVRNMACKTLELMAEGLKIQQRNAFSKLLM 180
T NS GFLS F Q PQKLRSA+N+YIS+VRNMAC+ LELMAEGLKIQQRNAFSKLLM
Sbjct: 121 THLESNSDGFLSIFGQDPQKLRSAVNDYISAVRNMACEILELMAEGLKIQQRNAFSKLLM 180
Query: 181 DEESDSVFRVNHYPPCPEEVRALEGKNMIGFGEHTDPQIISVLRSNNTSGLQISLADGNW 240
DEESDSVFRVNHYPPCP E++ L+G NMIGFGEHTDPQIISVLRSNNTSGLQISLADGNW
Sbjct: 181 DEESDSVFRVNHYPPCP-ELQTLKGTNMIGFGEHTDPQIISVLRSNNTSGLQISLADGNW 240
Query: 241 VSVPPDQNSFFINVGDSLQVMTNGRFKSVKHRVLTNSLKSRISMIYFGGPPLSEKIAPLP 300
+SVPPDQ+SFFINVGDSLQVMTNGRFKSVKHRVLTN LKSR+SMIYFGGPPLSEKIAPLP
Sbjct: 241 ISVPPDQSSFFINVGDSLQVMTNGRFKSVKHRVLTNRLKSRVSMIYFGGPPLSEKIAPLP 300
Query: 301 SLMKGEESLYKEFTWFEYKRSAYNSRLADNRLVHFERIAAS 327
SLMKGEESLYKEFTWFEYKRSAYNSRLADNRLVHFERIAAS
Sbjct: 301 SLMKGEESLYKEFTWFEYKRSAYNSRLADNRLVHFERIAAS 340
BLAST of Sed0020249 vs. NCBI nr
Match:
XP_022942236.1 (gibberellin 2-beta-dioxygenase 1-like [Cucurbita moschata])
HSP 1 Score: 568.2 bits (1463), Expect = 4.7e-158
Identity = 292/341 (85.63%), Postives = 309/341 (90.62%), Query Frame = 0
Query: 1 MVALSKPAIQKLSS-----------SSSSSSSSRVPLIDLSAPDAKHLIVKACEELGFFK 60
MV LS+PAI++++S SSS++ S VPLIDLSAPDAKHLIVKACEELGFFK
Sbjct: 1 MVVLSEPAIKQMASSYMRNSMAAAGSSSAAFFSGVPLIDLSAPDAKHLIVKACEELGFFK 60
Query: 61 VVNHGVPMEFISALESESTKFFSLPLSEKHRAGPASPFGYGNKQIGRNGDVGWVEYLLLQ 120
VVNHGVPMEFIS LESESTKFFSLPLSEK RAGP SPFGYGNKQIGRNGDVGWVEYLLL
Sbjct: 61 VVNHGVPMEFISTLESESTKFFSLPLSEKQRAGPPSPFGYGNKQIGRNGDVGWVEYLLLN 120
Query: 121 T----NSHGFLSTFAQHPQKLRSALNNYISSVRNMACKTLELMAEGLKIQQRNAFSKLLM 180
T NS GFLS F Q PQKLRSA+N+YIS+VRNMAC+ LELMAEGLKIQQRNAFSKLLM
Sbjct: 121 THLESNSDGFLSIFGQDPQKLRSAVNDYISAVRNMACEILELMAEGLKIQQRNAFSKLLM 180
Query: 181 DEESDSVFRVNHYPPCPEEVRALEGKNMIGFGEHTDPQIISVLRSNNTSGLQISLADGNW 240
DEESDSVFRVNHYPPCP E++ L+G NMIGFGEHTDPQIISVLRSNNTSGLQISLADGNW
Sbjct: 181 DEESDSVFRVNHYPPCP-ELQTLKGTNMIGFGEHTDPQIISVLRSNNTSGLQISLADGNW 240
Query: 241 VSVPPDQNSFFINVGDSLQVMTNGRFKSVKHRVLTNSLKSRISMIYFGGPPLSEKIAPLP 300
+SVPPDQ+SFFINVGDSLQVMTNGRFKSVKHRVLTN LKSR+SMIYFGGPPLSEKIAPLP
Sbjct: 241 ISVPPDQSSFFINVGDSLQVMTNGRFKSVKHRVLTNRLKSRVSMIYFGGPPLSEKIAPLP 300
Query: 301 SLMKGEESLYKEFTWFEYKRSAYNSRLADNRLVHFERIAAS 327
SLMKGEESLYKEFTWFEYKRSAYNSRLADNRLVHFERIAAS
Sbjct: 301 SLMKGEESLYKEFTWFEYKRSAYNSRLADNRLVHFERIAAS 340
BLAST of Sed0020249 vs. NCBI nr
Match:
XP_022981800.1 (gibberellin 2-beta-dioxygenase 1-like [Cucurbita maxima])
HSP 1 Score: 567.8 bits (1462), Expect = 6.2e-158
Identity = 292/341 (85.63%), Postives = 309/341 (90.62%), Query Frame = 0
Query: 1 MVALSKPAIQKLSS-----------SSSSSSSSRVPLIDLSAPDAKHLIVKACEELGFFK 60
MV LSKP+I++++S SSS++ S VPLIDLSAPDAKHLIVKACEELGFFK
Sbjct: 1 MVVLSKPSIKQMASSYMRNSMAAAGSSSAAFFSGVPLIDLSAPDAKHLIVKACEELGFFK 60
Query: 61 VVNHGVPMEFISALESESTKFFSLPLSEKHRAGPASPFGYGNKQIGRNGDVGWVEYLLLQ 120
VVNHGVPMEFIS LESESTKFFSLPLSEK RAGP SPFGYGNKQIGRNGDVGWVEYLLL
Sbjct: 61 VVNHGVPMEFISTLESESTKFFSLPLSEKQRAGPPSPFGYGNKQIGRNGDVGWVEYLLLN 120
Query: 121 TN----SHGFLSTFAQHPQKLRSALNNYISSVRNMACKTLELMAEGLKIQQRNAFSKLLM 180
T+ S GFLS F Q PQKLRSA+N+YIS+VRNMAC+ LELMAEGLKIQQRNAFSKLLM
Sbjct: 121 THLESKSDGFLSIFGQDPQKLRSAVNDYISAVRNMACEILELMAEGLKIQQRNAFSKLLM 180
Query: 181 DEESDSVFRVNHYPPCPEEVRALEGKNMIGFGEHTDPQIISVLRSNNTSGLQISLADGNW 240
DEESDSVFRVNHYPPCP E+ AL+G NMIGFGEHTDPQIISVLRSNNTSGLQISLADGNW
Sbjct: 181 DEESDSVFRVNHYPPCP-ELHALKGTNMIGFGEHTDPQIISVLRSNNTSGLQISLADGNW 240
Query: 241 VSVPPDQNSFFINVGDSLQVMTNGRFKSVKHRVLTNSLKSRISMIYFGGPPLSEKIAPLP 300
+SVPPDQ+SFFINVGDSLQVMTNGRFKSVKHRVLTN LKSR+SMIYFGGPPLSEKIAPLP
Sbjct: 241 ISVPPDQSSFFINVGDSLQVMTNGRFKSVKHRVLTNRLKSRVSMIYFGGPPLSEKIAPLP 300
Query: 301 SLMKGEESLYKEFTWFEYKRSAYNSRLADNRLVHFERIAAS 327
SLMKGEESLYKEFTWFEYKRSAYNSRLADNRLVHFERIAAS
Sbjct: 301 SLMKGEESLYKEFTWFEYKRSAYNSRLADNRLVHFERIAAS 340
BLAST of Sed0020249 vs. ExPASy Swiss-Prot
Match:
Q9SQ80 (Gibberellin 2-beta-dioxygenase 1 OS=Pisum sativum OX=3888 GN=GA2OX1 PE=1 SV=1)
HSP 1 Score: 457.6 bits (1176), Expect = 1.2e-127
Identity = 224/329 (68.09%), Postives = 270/329 (82.07%), Query Frame = 0
Query: 1 MVALSKPAIQK---LSSSSSSSSSSRVPLIDLSAPDAKHLIVKACEELGFFKVVNHGVPM 60
MV LSKP ++ + ++ + SS +PL+DLS PDAK LIVKACE+ GFFKV+NHG+P+
Sbjct: 1 MVLLSKPTSEQYTYVRNNMPITFSSSIPLVDLSKPDAKTLIVKACEDFGFFKVINHGIPL 60
Query: 61 EFISALESESTKFFSLPLSEKHRAGPASPFGYGNKQIGRNGDVGWVEYLLLQTNSHGFLS 120
+ IS LESE+ KFFSLP +EK +AGPA+PFGYGNK+IG NGD+GW+EYLLL TN S
Sbjct: 61 DAISQLESEAFKFFSLPQTEKEKAGPANPFGYGNKRIGLNGDIGWIEYLLLTTNQDHNFS 120
Query: 121 TFAQHPQKLRSALNNYISSVRNMACKTLELMAEGLKIQQRNAFSKLLMDEESDSVFRVNH 180
+ + K R L +Y ++RNMAC+ L+LMAEGLKIQ +N FSKL+MD++SD +FRVNH
Sbjct: 121 LYGEDIDKFRGLLKDYKCAMRNMACEILDLMAEGLKIQPKNVFSKLVMDKQSDCLFRVNH 180
Query: 181 YPPCPEEVRALEGKNMIGFGEHTDPQIISVLRSNNTSGLQISLADGNWVSVPPDQNSFFI 240
YP CPE A+ G+N+IGFGEHTDPQIIS+LRSNNTSG QISL DG+W+SVPPD +SFFI
Sbjct: 181 YPACPE--LAINGENLIGFGEHTDPQIISILRSNNTSGFQISLRDGSWISVPPDHSSFFI 240
Query: 241 NVGDSLQVMTNGRFKSVKHRVLTNSLKSRISMIYFGGPPLSEKIAPLPSLMKGEESLYKE 300
NVGDSLQVMTNGRFKSV+HRVL N + R+SMIYF GPPLSEKIAPLPSLMKG+ESLYKE
Sbjct: 241 NVGDSLQVMTNGRFKSVRHRVLANGIDPRLSMIYFCGPPLSEKIAPLPSLMKGKESLYKE 300
Query: 301 FTWFEYKRSAYNSRLADNRLVHFERIAAS 327
FTWFEYK S Y SRLADNRL ++ERIAA+
Sbjct: 301 FTWFEYKSSTYGSRLADNRLGNYERIAAT 327
BLAST of Sed0020249 vs. ExPASy Swiss-Prot
Match:
Q9XG83 (Gibberellin 2-beta-dioxygenase OS=Phaseolus coccineus OX=3886 GN=GA2OX1 PE=2 SV=1)
HSP 1 Score: 423.3 bits (1087), Expect = 2.5e-117
Identity = 217/333 (65.17%), Postives = 262/333 (78.68%), Query Frame = 0
Query: 1 MVALSKPAIQK---LSSSSSSSSSSRVPLIDLSAPDAKHLIVKACEELGFFKVVNHGVPM 60
MV LS+PA+ + L S+ + +P++DL+ PDAK+LIV AC + GFFK+VNHGVP+
Sbjct: 1 MVVLSQPALNQFFLLKPFKSTPLFTGIPVVDLTHPDAKNLIVNACRDFGFFKLVNHGVPL 60
Query: 61 EFISALESESTKFFSLPLSEKHRAGPASPFGYGNKQIGRNGDVGWVEYLLLQTN----SH 120
E ++ LE+E+ +FF SEK RAGP PFGYG+K+IG NGDVGWVEYLLL TN S
Sbjct: 61 ELMANLENEALRFFKKSQSEKDRAGPPDPFGYGSKRIGPNGDVGWVEYLLLNTNPDVISP 120
Query: 121 GFLSTFAQHPQKLRSALNNYISSVRNMACKTLELMAEGLKIQQRNAFSKLLMDEESDSVF 180
L F ++P R+ + NYI++V+NM LELMAEGL I+QRN S+LL DE+SDS F
Sbjct: 121 KSLCIFRENPHHFRAVVENYITAVKNMCYAVLELMAEGLGIRQRNTLSRLLKDEKSDSCF 180
Query: 181 RVNHYPPCPEEVRALEGKNMIGFGEHTDPQIISVLRSNNTSGLQISLADGNWVSVPPDQN 240
R+NHYPPCP EV+AL +N++GFGEHTDPQIISVLRSN+TSGLQI L DG WVSVPPDQ
Sbjct: 181 RLNHYPPCP-EVQAL-NRNLVGFGEHTDPQIISVLRSNSTSGLQICLTDGTWVSVPPDQT 240
Query: 241 SFFINVGDSLQVMTNGRFKSVKHRVLTNSLKSRISMIYFGGPPLSEKIAPLPSLM-KGEE 300
SFFINVGD+LQVMTNGRFKSVKHRVL ++ KSR+SMIYFGGP LSE IAPLPS+M KGEE
Sbjct: 241 SFFINVGDALQVMTNGRFKSVKHRVLADTTKSRLSMIYFGGPALSENIAPLPSVMLKGEE 300
Query: 301 SLYKEFTWFEYKRSAYNSRLADNRLVHFERIAA 326
LYKEFTW EYK++AY SRLADNRL F++ AA
Sbjct: 301 CLYKEFTWCEYKKAAYTSRLADNRLAPFQKSAA 331
BLAST of Sed0020249 vs. ExPASy Swiss-Prot
Match:
Q8LEA2 (Gibberellin 2-beta-dioxygenase 1 OS=Arabidopsis thaliana OX=3702 GN=GA2OX1 PE=2 SV=2)
HSP 1 Score: 381.3 bits (978), Expect = 1.1e-104
Identity = 198/329 (60.18%), Postives = 236/329 (71.73%), Query Frame = 0
Query: 1 MVALSKP-AIQKLSSSSSSSSSSRVPLIDLSAPDAKHLIVKACEELGFFKVVNHGVPMEF 60
M LSKP AI K S S +P+ID+S P++KH +VKACE+ GFFKV+NHGV E
Sbjct: 1 MAVLSKPVAIPK-------SGFSLIPVIDMSDPESKHALVKACEDFGFFKVINHGVSAEL 60
Query: 61 ISALESESTKFFSLPLSEKHRAGPASPFGYGNKQIGRNGDVGWVEYLLLQTN----SHGF 120
+S LE E+ FFSLP SEK + PFGYGN +IGRNGDVGWVEYLL+ N S
Sbjct: 61 VSVLEHETVDFFSLPKSEKTQVA-GYPFGYGNSKIGRNGDVGWVEYLLMNANHDSGSGPL 120
Query: 121 LSTFAQHPQKLRSALNNYISSVRNMACKTLELMAEGLKIQQRNAFSKLLMDEESDSVFRV 180
+ + P R+AL Y +SVR M LE + +GL I+ RN SKL+ D+ +DS+ R+
Sbjct: 121 FPSLLKSPGTFRNALEEYTTSVRKMTFDVLEKITDGLGIKPRNTLSKLVSDQNTDSILRL 180
Query: 181 NHYPPCP-EEVRALEGKNMIGFGEHTDPQIISVLRSNNTSGLQISLADGNWVSVPPDQNS 240
NHYPPCP + GKN+IGFGEHTDPQIISVLRSNNTSGLQI+L DG+W+SVPPD S
Sbjct: 181 NHYPPCPLSNKKTNGGKNVIGFGEHTDPQIISVLRSNNTSGLQINLNDGSWISVPPDHTS 240
Query: 241 FFINVGDSLQVMTNGRFKSVKHRVLTNSLKSRISMIYFGGPPLSEKIAPLPSLMKGE-ES 300
FF NVGDSLQVMTNGRFKSV+HRVL N KSR+SMIYF GP L+++IAPL L+ E E
Sbjct: 241 FFFNVGDSLQVMTNGRFKSVRHRVLANCKKSRVSMIYFAGPSLTQRIAPLTCLIDNEDER 300
Query: 301 LYKEFTWFEYKRSAYNSRLADNRLVHFER 323
LY+EFTW EYK S YNSRL+DNRL FER
Sbjct: 301 LYEEFTWSEYKNSTYNSRLSDNRLQQFER 321
BLAST of Sed0020249 vs. ExPASy Swiss-Prot
Match:
Q8S0S6 (Gibberellin 2-beta-dioxygenase 3 OS=Oryza sativa subsp. japonica OX=39947 GN=GA2OX3 PE=1 SV=1)
HSP 1 Score: 366.7 bits (940), Expect = 2.7e-100
Identity = 194/331 (58.61%), Postives = 239/331 (72.21%), Query Frame = 0
Query: 1 MVALSKPA----IQKLSSSSSSSSSSRVPLIDLSAPDAKHLIVKACEELGFFKVVNHGVP 60
MV L+ P I L S S VP++DL +P A +V ACE GFFKVVNHGV
Sbjct: 1 MVVLAGPPAVDHIPLLRSPDPGDVFSGVPVVDLGSPGAARAVVDACERYGFFKVVNHGVA 60
Query: 61 MEFISALESESTKFFSLPLSEKHRAGPASPFGYGNKQIGRNGDVGWVEYLLLQTNSHGFL 120
+ + ESE+ +FFS +K R+GPA PFGYG+K+IG NGD+GW+EYLLL + L
Sbjct: 61 TDTMDKAESEAVRFFSQTQPDKDRSGPAYPFGYGSKRIGFNGDMGWLEYLLLALDD-ASL 120
Query: 121 STFAQHPQ--KLRSALNNYISSVRNMACKTLELMAEGLKIQQRNAFSKLLMDEESDSVFR 180
+ P R+ALN YIS VR +A + +E M+EGL I Q +A S L+ E SD VFR
Sbjct: 121 ADACTVPSCAVFRAALNEYISGVRKVAVRVMEAMSEGLGIAQADALSALVTAEGSDQVFR 180
Query: 181 VNHYPPCPEEVRALEGK--NMIGFGEHTDPQIISVLRSNNTSGLQISLADGNWVSVPPDQ 240
VNHYPPC RAL+G ++ GFGEHTDPQ++SVLRSN TSGLQI+L DG WVSVP D+
Sbjct: 181 VNHYPPC----RALQGLGCSVTGFGEHTDPQLVSVLRSNGTSGLQIALRDGQWVSVPSDR 240
Query: 241 NSFFINVGDSLQVMTNGRFKSVKHRVLTNSLKSRISMIYFGGPPLSEKIAPLPSLM-KGE 300
+SFF+NVGDSLQV+TNGRFKSVKHRV+ NSLKSR+S IYFGGPPL+++IAPLP L+ +GE
Sbjct: 241 DSFFVNVGDSLQVLTNGRFKSVKHRVVANSLKSRVSFIYFGGPPLAQRIAPLPQLLGEGE 300
Query: 301 ESLYKEFTWFEYKRSAYNSRLADNRLVHFER 323
+SLYKEFTW EYK++AY SRL DNRL FE+
Sbjct: 301 QSLYKEFTWDEYKKAAYKSRLGDNRLAQFEK 326
BLAST of Sed0020249 vs. ExPASy Swiss-Prot
Match:
Q9XFR9 (Gibberellin 2-beta-dioxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=GA2OX2 PE=2 SV=1)
HSP 1 Score: 365.2 bits (936), Expect = 7.9e-100
Identity = 184/308 (59.74%), Postives = 230/308 (74.68%), Query Frame = 0
Query: 20 SSSRVPLIDLSAPDAKHLIVKACEELGFFKVVNHGVPMEFISALESESTKFFSLPLSEKH 79
+S +P+++L+ P+AK IVKACEE GFFKVVNHGV E ++ LE E+ FF LP S K+
Sbjct: 27 TSHSIPVVNLADPEAKTRIVKACEEFGFFKVVNHGVRPELMTRLEQEAIGFFGLPQSLKN 86
Query: 80 RAGPASPFGYGNKQIGRNGDVGWVEYLLLQTN----SHGFLSTFAQHPQKLRSALNNYIS 139
RAGP P+GYGNK+IG NGDVGW+EYLLL N S + F Q PQ R ++ Y+
Sbjct: 87 RAGPPEPYGYGNKRIGPNGDVGWIEYLLLNANPQLSSPKTSAVFRQTPQIFRESVEEYMK 146
Query: 140 SVRNMACKTLELMAEGLKIQQRNAFSKLLMDEESDSVFRVNHYPPCPEEVRALEGKNMIG 199
++ ++ K LE++AE L I+ R+ SK+L DE+SDS R+NHYP EE E +G
Sbjct: 147 EIKEVSYKVLEMVAEELGIEPRDTLSKMLRDEKSDSCLRLNHYPAAEEEA---EKMVKVG 206
Query: 200 FGEHTDPQIISVLRSNNTSGLQISLADGNWVSVPPDQNSFFINVGDSLQVMTNGRFKSVK 259
FGEHTDPQIISVLRSNNT+GLQI + DG+WV+VPPD +SFFINVGD+LQVMTNGRFKSVK
Sbjct: 207 FGEHTDPQIISVLRSNNTAGLQICVKDGSWVAVPPDHSSFFINVGDALQVMTNGRFKSVK 266
Query: 260 HRVLTNSLKSRISMIYFGGPPLSEKIAPLPSLMKGEES-LYKEFTWFEYKRSAYNSRLAD 319
HRVL ++ +SRISMIYFGGPPLS+KIAPLP L+ ++ LYKEFTW +YK SAY S+L D
Sbjct: 267 HRVLADTRRSRISMIYFGGPPLSQKIAPLPCLVPEQDDWLYKEFTWSQYKSSAYKSKLGD 326
Query: 320 NRLVHFER 323
RL FE+
Sbjct: 327 YRLGLFEK 331
BLAST of Sed0020249 vs. ExPASy TrEMBL
Match:
O04162 (Dioxygenase OS=Marah macrocarpa OX=48242 PE=2 SV=1)
HSP 1 Score: 582.8 bits (1501), Expect = 8.9e-163
Identity = 294/326 (90.18%), Postives = 304/326 (93.25%), Query Frame = 0
Query: 1 MVALSKPAIQKLSSSSSSSSSSRVPLIDLSAPDAKHLIVKACEELGFFKVVNHGVPMEFI 60
MV LSKP IQ+LSSSSSS VPLIDLSAPDAKHLIVKACEELGFFKV+NHGVPMEFI
Sbjct: 1 MVVLSKPEIQQLSSSSSSG----VPLIDLSAPDAKHLIVKACEELGFFKVINHGVPMEFI 60
Query: 61 SALESESTKFFSLPLSEKHRAGPASPFGYGNKQIGRNGDVGWVEYLLLQTNSHGFLSTFA 120
S LESESTKFFSLPLSEK RAGP SPFGYGNKQIGRNGDVGWVEYLLLQ+NSH FLS F
Sbjct: 61 STLESESTKFFSLPLSEKQRAGPPSPFGYGNKQIGRNGDVGWVEYLLLQSNSHAFLSIFG 120
Query: 121 QHPQKLRSALNNYISSVRNMACKTLELMAEGLKIQQRNAFSKLLMDEESDSVFRVNHYPP 180
Q PQKLRSALN+YI +VRNMAC+ +ELMAEGLKIQQRNA SKLLM EESDSVFRVNHYPP
Sbjct: 121 QDPQKLRSALNDYIWAVRNMACEIVELMAEGLKIQQRNALSKLLMGEESDSVFRVNHYPP 180
Query: 181 CPEEVRALEGKNMIGFGEHTDPQIISVLRSNNTSGLQISLADGNWVSVPPDQNSFFINVG 240
CPEE++ALEG NMIGFGEHTDPQIISVLRSNNTSGLQISL D NW+SVPPDQ SFFINVG
Sbjct: 181 CPEELQALEGTNMIGFGEHTDPQIISVLRSNNTSGLQISLPDANWISVPPDQTSFFINVG 240
Query: 241 DSLQVMTNGRFKSVKHRVLTNSLKSRISMIYFGGPPLSEKIAPLPSLMKGEESLYKEFTW 300
DSLQVMTNGRFKSVKHRVLTNSLKSRISMIYFGGPPLSEKIAPLPSLMKGEESLYKEFTW
Sbjct: 241 DSLQVMTNGRFKSVKHRVLTNSLKSRISMIYFGGPPLSEKIAPLPSLMKGEESLYKEFTW 300
Query: 301 FEYKRSAYNSRLADNRLVHFERIAAS 327
FEYKRSAYNSRLADNRLVHFERIAAS
Sbjct: 301 FEYKRSAYNSRLADNRLVHFERIAAS 322
BLAST of Sed0020249 vs. ExPASy TrEMBL
Match:
A0A6J1FNB4 (gibberellin 2-beta-dioxygenase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111447347 PE=3 SV=1)
HSP 1 Score: 568.2 bits (1463), Expect = 2.3e-158
Identity = 292/341 (85.63%), Postives = 309/341 (90.62%), Query Frame = 0
Query: 1 MVALSKPAIQKLSS-----------SSSSSSSSRVPLIDLSAPDAKHLIVKACEELGFFK 60
MV LS+PAI++++S SSS++ S VPLIDLSAPDAKHLIVKACEELGFFK
Sbjct: 1 MVVLSEPAIKQMASSYMRNSMAAAGSSSAAFFSGVPLIDLSAPDAKHLIVKACEELGFFK 60
Query: 61 VVNHGVPMEFISALESESTKFFSLPLSEKHRAGPASPFGYGNKQIGRNGDVGWVEYLLLQ 120
VVNHGVPMEFIS LESESTKFFSLPLSEK RAGP SPFGYGNKQIGRNGDVGWVEYLLL
Sbjct: 61 VVNHGVPMEFISTLESESTKFFSLPLSEKQRAGPPSPFGYGNKQIGRNGDVGWVEYLLLN 120
Query: 121 T----NSHGFLSTFAQHPQKLRSALNNYISSVRNMACKTLELMAEGLKIQQRNAFSKLLM 180
T NS GFLS F Q PQKLRSA+N+YIS+VRNMAC+ LELMAEGLKIQQRNAFSKLLM
Sbjct: 121 THLESNSDGFLSIFGQDPQKLRSAVNDYISAVRNMACEILELMAEGLKIQQRNAFSKLLM 180
Query: 181 DEESDSVFRVNHYPPCPEEVRALEGKNMIGFGEHTDPQIISVLRSNNTSGLQISLADGNW 240
DEESDSVFRVNHYPPCP E++ L+G NMIGFGEHTDPQIISVLRSNNTSGLQISLADGNW
Sbjct: 181 DEESDSVFRVNHYPPCP-ELQTLKGTNMIGFGEHTDPQIISVLRSNNTSGLQISLADGNW 240
Query: 241 VSVPPDQNSFFINVGDSLQVMTNGRFKSVKHRVLTNSLKSRISMIYFGGPPLSEKIAPLP 300
+SVPPDQ+SFFINVGDSLQVMTNGRFKSVKHRVLTN LKSR+SMIYFGGPPLSEKIAPLP
Sbjct: 241 ISVPPDQSSFFINVGDSLQVMTNGRFKSVKHRVLTNRLKSRVSMIYFGGPPLSEKIAPLP 300
Query: 301 SLMKGEESLYKEFTWFEYKRSAYNSRLADNRLVHFERIAAS 327
SLMKGEESLYKEFTWFEYKRSAYNSRLADNRLVHFERIAAS
Sbjct: 301 SLMKGEESLYKEFTWFEYKRSAYNSRLADNRLVHFERIAAS 340
BLAST of Sed0020249 vs. ExPASy TrEMBL
Match:
A0A6J1J0N2 (gibberellin 2-beta-dioxygenase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111480835 PE=3 SV=1)
HSP 1 Score: 567.8 bits (1462), Expect = 3.0e-158
Identity = 292/341 (85.63%), Postives = 309/341 (90.62%), Query Frame = 0
Query: 1 MVALSKPAIQKLSS-----------SSSSSSSSRVPLIDLSAPDAKHLIVKACEELGFFK 60
MV LSKP+I++++S SSS++ S VPLIDLSAPDAKHLIVKACEELGFFK
Sbjct: 1 MVVLSKPSIKQMASSYMRNSMAAAGSSSAAFFSGVPLIDLSAPDAKHLIVKACEELGFFK 60
Query: 61 VVNHGVPMEFISALESESTKFFSLPLSEKHRAGPASPFGYGNKQIGRNGDVGWVEYLLLQ 120
VVNHGVPMEFIS LESESTKFFSLPLSEK RAGP SPFGYGNKQIGRNGDVGWVEYLLL
Sbjct: 61 VVNHGVPMEFISTLESESTKFFSLPLSEKQRAGPPSPFGYGNKQIGRNGDVGWVEYLLLN 120
Query: 121 TN----SHGFLSTFAQHPQKLRSALNNYISSVRNMACKTLELMAEGLKIQQRNAFSKLLM 180
T+ S GFLS F Q PQKLRSA+N+YIS+VRNMAC+ LELMAEGLKIQQRNAFSKLLM
Sbjct: 121 THLESKSDGFLSIFGQDPQKLRSAVNDYISAVRNMACEILELMAEGLKIQQRNAFSKLLM 180
Query: 181 DEESDSVFRVNHYPPCPEEVRALEGKNMIGFGEHTDPQIISVLRSNNTSGLQISLADGNW 240
DEESDSVFRVNHYPPCP E+ AL+G NMIGFGEHTDPQIISVLRSNNTSGLQISLADGNW
Sbjct: 181 DEESDSVFRVNHYPPCP-ELHALKGTNMIGFGEHTDPQIISVLRSNNTSGLQISLADGNW 240
Query: 241 VSVPPDQNSFFINVGDSLQVMTNGRFKSVKHRVLTNSLKSRISMIYFGGPPLSEKIAPLP 300
+SVPPDQ+SFFINVGDSLQVMTNGRFKSVKHRVLTN LKSR+SMIYFGGPPLSEKIAPLP
Sbjct: 241 ISVPPDQSSFFINVGDSLQVMTNGRFKSVKHRVLTNRLKSRVSMIYFGGPPLSEKIAPLP 300
Query: 301 SLMKGEESLYKEFTWFEYKRSAYNSRLADNRLVHFERIAAS 327
SLMKGEESLYKEFTWFEYKRSAYNSRLADNRLVHFERIAAS
Sbjct: 301 SLMKGEESLYKEFTWFEYKRSAYNSRLADNRLVHFERIAAS 340
BLAST of Sed0020249 vs. ExPASy TrEMBL
Match:
A0A1S3BRQ7 (gibberellin 2-beta-dioxygenase 1 OS=Cucumis melo OX=3656 GN=LOC103492969 PE=3 SV=1)
HSP 1 Score: 560.5 bits (1443), Expect = 4.8e-156
Identity = 291/343 (84.84%), Postives = 307/343 (89.50%), Query Frame = 0
Query: 1 MVALSKPAIQKLSS------------SSSSSSSSRVPLIDLSAPDAKHLIVKACEELGFF 60
MV LSK +IQ+LSS SSS++ S VPLIDLS+PDAK LIVKACEELGFF
Sbjct: 1 MVVLSKQSIQQLSSNSFMRNSMASAASSSAAFFSEVPLIDLSSPDAKQLIVKACEELGFF 60
Query: 61 KVVNHGVPMEFISALESESTKFFSLPLSEKHRAGPASPFGYGNKQIGRNGDVGWVEYLLL 120
KVVNHGVPMEFIS LESEST FFSLPLSEK +A P SPFGYGNKQIGRNGDVGWVEY+LL
Sbjct: 61 KVVNHGVPMEFISTLESESTNFFSLPLSEKQKAAPPSPFGYGNKQIGRNGDVGWVEYILL 120
Query: 121 QT----NSHGFLSTFAQHPQKLRSALNNYISSVRNMACKTLELMAEGLKIQQRNAFSKLL 180
T NS GFLS F Q PQKLRSA+NNYIS+VRNMAC+ LELMAEGLKIQQRNAFSKL+
Sbjct: 121 NTHLESNSDGFLSIFGQDPQKLRSAVNNYISAVRNMACEILELMAEGLKIQQRNAFSKLV 180
Query: 181 MDEESDSVFRVNHYPPCPEEVRALEGKNMIGFGEHTDPQIISVLRSNNTSGLQISLADGN 240
MDEESDSVFRVNHYPPCP +++AL+G NMIGFGEHTDPQIISVLRSNNTSGLQISLADGN
Sbjct: 181 MDEESDSVFRVNHYPPCP-QIQALKGMNMIGFGEHTDPQIISVLRSNNTSGLQISLADGN 240
Query: 241 WVSVPPDQNSFFINVGDSLQVMTNGRFKSVKHRVLTNSLKSRISMIYFGGPPLSEKIAPL 300
W+SVPPDQNSFFINVGDSLQVMTNGRFKSVKHRVLTNSLKSRISMIYFGGPPLSEKIAPL
Sbjct: 241 WISVPPDQNSFFINVGDSLQVMTNGRFKSVKHRVLTNSLKSRISMIYFGGPPLSEKIAPL 300
Query: 301 PSLMKGEE-SLYKEFTWFEYKRSAYNSRLADNRLVHFERIAAS 327
PSLMKGEE SLYKEFTWFEYKRSAYNSRLADNRLVHFERIAAS
Sbjct: 301 PSLMKGEERSLYKEFTWFEYKRSAYNSRLADNRLVHFERIAAS 342
BLAST of Sed0020249 vs. ExPASy TrEMBL
Match:
A0A0A0KXI6 (Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G075200 PE=3 SV=1)
HSP 1 Score: 557.8 bits (1436), Expect = 3.1e-155
Identity = 290/343 (84.55%), Postives = 305/343 (88.92%), Query Frame = 0
Query: 1 MVALSKPAIQKLSS------------SSSSSSSSRVPLIDLSAPDAKHLIVKACEELGFF 60
MV LSK +IQ+LSS SSS + S VPLIDLS+PDAK LIVKACEELGFF
Sbjct: 1 MVILSKQSIQQLSSNSFMRNSMASAASSSPAFLSEVPLIDLSSPDAKQLIVKACEELGFF 60
Query: 61 KVVNHGVPMEFISALESESTKFFSLPLSEKHRAGPASPFGYGNKQIGRNGDVGWVEYLLL 120
KVVNHGVPMEFIS LESEST FFSLPLSEK +A P SPFGYGNKQIGRNGDVGWVEY+LL
Sbjct: 61 KVVNHGVPMEFISTLESESTNFFSLPLSEKQKAAPPSPFGYGNKQIGRNGDVGWVEYILL 120
Query: 121 QT----NSHGFLSTFAQHPQKLRSALNNYISSVRNMACKTLELMAEGLKIQQRNAFSKLL 180
T NS GFLS F Q PQKLRSA+NNYIS+VRNMAC+ LELMAEGLKIQQRN FSKL+
Sbjct: 121 NTHLESNSDGFLSIFGQDPQKLRSAVNNYISAVRNMACEILELMAEGLKIQQRNVFSKLV 180
Query: 181 MDEESDSVFRVNHYPPCPEEVRALEGKNMIGFGEHTDPQIISVLRSNNTSGLQISLADGN 240
MDEESDSVFRVNHYPPCP +++AL+G NMIGFGEHTDPQIISVLRSNNTSGLQISLADGN
Sbjct: 181 MDEESDSVFRVNHYPPCP-QIQALKGMNMIGFGEHTDPQIISVLRSNNTSGLQISLADGN 240
Query: 241 WVSVPPDQNSFFINVGDSLQVMTNGRFKSVKHRVLTNSLKSRISMIYFGGPPLSEKIAPL 300
W+SVPPDQNSFFINVGDSLQVMTNGRFKSVKHRVLTNSLKSRISMIYFGGPPLSEKIAPL
Sbjct: 241 WISVPPDQNSFFINVGDSLQVMTNGRFKSVKHRVLTNSLKSRISMIYFGGPPLSEKIAPL 300
Query: 301 PSLMKGEE-SLYKEFTWFEYKRSAYNSRLADNRLVHFERIAAS 327
PSLMKGEE SLYKEFTWFEYKRSAYNSRLADNRLVHFERIAAS
Sbjct: 301 PSLMKGEERSLYKEFTWFEYKRSAYNSRLADNRLVHFERIAAS 342
BLAST of Sed0020249 vs. TAIR 10
Match:
AT1G78440.1 (Arabidopsis thaliana gibberellin 2-oxidase 1 )
HSP 1 Score: 381.3 bits (978), Expect = 7.6e-106
Identity = 198/329 (60.18%), Postives = 236/329 (71.73%), Query Frame = 0
Query: 1 MVALSKP-AIQKLSSSSSSSSSSRVPLIDLSAPDAKHLIVKACEELGFFKVVNHGVPMEF 60
M LSKP AI K S S +P+ID+S P++KH +VKACE+ GFFKV+NHGV E
Sbjct: 1 MAVLSKPVAIPK-------SGFSLIPVIDMSDPESKHALVKACEDFGFFKVINHGVSAEL 60
Query: 61 ISALESESTKFFSLPLSEKHRAGPASPFGYGNKQIGRNGDVGWVEYLLLQTN----SHGF 120
+S LE E+ FFSLP SEK + PFGYGN +IGRNGDVGWVEYLL+ N S
Sbjct: 61 VSVLEHETVDFFSLPKSEKTQVA-GYPFGYGNSKIGRNGDVGWVEYLLMNANHDSGSGPL 120
Query: 121 LSTFAQHPQKLRSALNNYISSVRNMACKTLELMAEGLKIQQRNAFSKLLMDEESDSVFRV 180
+ + P R+AL Y +SVR M LE + +GL I+ RN SKL+ D+ +DS+ R+
Sbjct: 121 FPSLLKSPGTFRNALEEYTTSVRKMTFDVLEKITDGLGIKPRNTLSKLVSDQNTDSILRL 180
Query: 181 NHYPPCP-EEVRALEGKNMIGFGEHTDPQIISVLRSNNTSGLQISLADGNWVSVPPDQNS 240
NHYPPCP + GKN+IGFGEHTDPQIISVLRSNNTSGLQI+L DG+W+SVPPD S
Sbjct: 181 NHYPPCPLSNKKTNGGKNVIGFGEHTDPQIISVLRSNNTSGLQINLNDGSWISVPPDHTS 240
Query: 241 FFINVGDSLQVMTNGRFKSVKHRVLTNSLKSRISMIYFGGPPLSEKIAPLPSLMKGE-ES 300
FF NVGDSLQVMTNGRFKSV+HRVL N KSR+SMIYF GP L+++IAPL L+ E E
Sbjct: 241 FFFNVGDSLQVMTNGRFKSVRHRVLANCKKSRVSMIYFAGPSLTQRIAPLTCLIDNEDER 300
Query: 301 LYKEFTWFEYKRSAYNSRLADNRLVHFER 323
LY+EFTW EYK S YNSRL+DNRL FER
Sbjct: 301 LYEEFTWSEYKNSTYNSRLSDNRLQQFER 321
BLAST of Sed0020249 vs. TAIR 10
Match:
AT1G30040.1 (gibberellin 2-oxidase )
HSP 1 Score: 365.2 bits (936), Expect = 5.6e-101
Identity = 184/308 (59.74%), Postives = 230/308 (74.68%), Query Frame = 0
Query: 20 SSSRVPLIDLSAPDAKHLIVKACEELGFFKVVNHGVPMEFISALESESTKFFSLPLSEKH 79
+S +P+++L+ P+AK IVKACEE GFFKVVNHGV E ++ LE E+ FF LP S K+
Sbjct: 27 TSHSIPVVNLADPEAKTRIVKACEEFGFFKVVNHGVRPELMTRLEQEAIGFFGLPQSLKN 86
Query: 80 RAGPASPFGYGNKQIGRNGDVGWVEYLLLQTN----SHGFLSTFAQHPQKLRSALNNYIS 139
RAGP P+GYGNK+IG NGDVGW+EYLLL N S + F Q PQ R ++ Y+
Sbjct: 87 RAGPPEPYGYGNKRIGPNGDVGWIEYLLLNANPQLSSPKTSAVFRQTPQIFRESVEEYMK 146
Query: 140 SVRNMACKTLELMAEGLKIQQRNAFSKLLMDEESDSVFRVNHYPPCPEEVRALEGKNMIG 199
++ ++ K LE++AE L I+ R+ SK+L DE+SDS R+NHYP EE E +G
Sbjct: 147 EIKEVSYKVLEMVAEELGIEPRDTLSKMLRDEKSDSCLRLNHYPAAEEEA---EKMVKVG 206
Query: 200 FGEHTDPQIISVLRSNNTSGLQISLADGNWVSVPPDQNSFFINVGDSLQVMTNGRFKSVK 259
FGEHTDPQIISVLRSNNT+GLQI + DG+WV+VPPD +SFFINVGD+LQVMTNGRFKSVK
Sbjct: 207 FGEHTDPQIISVLRSNNTAGLQICVKDGSWVAVPPDHSSFFINVGDALQVMTNGRFKSVK 266
Query: 260 HRVLTNSLKSRISMIYFGGPPLSEKIAPLPSLMKGEES-LYKEFTWFEYKRSAYNSRLAD 319
HRVL ++ +SRISMIYFGGPPLS+KIAPLP L+ ++ LYKEFTW +YK SAY S+L D
Sbjct: 267 HRVLADTRRSRISMIYFGGPPLSQKIAPLPCLVPEQDDWLYKEFTWSQYKSSAYKSKLGD 326
Query: 320 NRLVHFER 323
RL FE+
Sbjct: 327 YRLGLFEK 331
BLAST of Sed0020249 vs. TAIR 10
Match:
AT2G34555.1 (gibberellin 2-oxidase 3 )
HSP 1 Score: 335.5 bits (859), Expect = 4.8e-92
Identity = 171/304 (56.25%), Postives = 215/304 (70.72%), Query Frame = 0
Query: 24 VPLIDLSAPDAKHLIVKACEELGFFKVVNHGVPMEFISALESESTKFFSLPLSEKHRAGP 83
+P+IDL+ DAK IVKACEE GFFKV+NHGV + ++ LE E+ FF+L S K +AGP
Sbjct: 27 IPVIDLTDSDAKTQIVKACEEFGFFKVINHGVRPDLLTQLEQEAINFFALHHSLKDKAGP 86
Query: 84 ASPFGYGNKQIGRNGDVGWVEYLLLQTN----SHGFLSTFAQHPQKLRSALNNYISSVRN 143
PFGYG K+IG NGD+GW+EY+LL N SH + F P R A+ YI ++
Sbjct: 87 PDPFGYGTKRIGPNGDLGWLEYILLNANLCLESHKTTAIFRHTPAIFREAVEEYIKEMKR 146
Query: 144 MACKTLELMAEGLKIQQRNAFSKLLMDEESDSVFRVNHYPPCPEEVRALEGKNMIGFGEH 203
M+ K LE++ E LKI+ + S+L+ +ESDS R+NHYP E K IGFGEH
Sbjct: 147 MSSKFLEMVEEELKIEPKEKLSRLVKVKESDSCLRMNHYP----EKEETPVKEEIGFGEH 206
Query: 204 TDPQIISVLRSNNTSGLQISLADGNWVSVPPDQNSFFINVGDSLQVMTNGRFKSVKHRVL 263
TDPQ+IS+LRSN+T GLQI + DG WV V PD +SFF+ VGD+LQVMTNGRFKSVKHRV+
Sbjct: 207 TDPQLISLLRSNDTEGLQICVKDGTWVDVTPDHSSFFVLVGDTLQVMTNGRFKSVKHRVV 266
Query: 264 TNSLKSRISMIYFGGPPLSEKIAPLPSLM-KGEESLYKEFTWFEYKRSAYNSRLADNRLV 323
TN+ +SRISMIYF GPPLSEKIAPL L+ K ++ LY EFTW +YK SAY ++L D RL
Sbjct: 267 TNTKRSRISMIYFAGPPLSEKIAPLSCLVPKQDDCLYNEFTWSQYKLSAYKTKLGDYRLG 326
BLAST of Sed0020249 vs. TAIR 10
Match:
AT1G02400.1 (gibberellin 2-oxidase 6 )
HSP 1 Score: 288.1 bits (736), Expect = 8.8e-78
Identity = 161/332 (48.49%), Postives = 205/332 (61.75%), Query Frame = 0
Query: 1 MVALSKPAIQKLSSSSSSSSSSRVPLIDLSAPDAKHL---IVKACEELGFFKVVNHGVPM 60
MV S +Q + SS P+ID S D L IVKACE GFFKV+NHGV
Sbjct: 1 MVLPSSTPLQTTGKKTISSPEYNFPVIDFSLNDRSKLSEKIVKACEVNGFFKVINHGVKP 60
Query: 61 EFISALESESTKFFSLPLSEKHRAGPASPFGYGNKQIGRNGDVGWVEYLLLQTNSHGFL- 120
E I E E +FF+ P S+K RAGPASPFGYG K IG NGD+G +EYLLL N
Sbjct: 61 EIIKRFEHEGEEFFNKPESDKLRAGPASPFGYGCKNIGFNGDLGELEYLLLHANPTAVAD 120
Query: 121 -STFAQH--PQKLRSALNNYISSVRNMACKTLELMAEGLKIQQRNAFSKLLMDEESDSVF 180
S H P K SA N+YI +VR++AC+ ++L E L Q+ + S+L+ D SDS+
Sbjct: 121 KSETISHDDPFKFSSATNDYIRTVRDLACEIIDLTIENLWGQKSSEVSELIRDVRSDSIL 180
Query: 181 RVNHYPPCPEEVRALEGKNMIGFGEHTDPQIISVLRSNNTSGLQISLADGNWVSVPPDQN 240
R+NHYPP P AL G IGFGEH+DPQI++VLRSN+ GL+I DG W+ +P D
Sbjct: 181 RLNHYPPAP---YALSGVGQIGFGEHSDPQILTVLRSNDVDGLEICSRDGLWIPIPSDPT 240
Query: 241 SFFINVGDSLQVMTNGRFKSVKHRVLTNSLKS-RISMIYFGGPPLSEKIAPLPSLMKGEE 300
FF+ VGD LQ +TNGRF SV+HRVL N+ K R+S +YF PPL KI+PLP ++ E
Sbjct: 241 CFFVLVGDCLQALTNGRFTSVRHRVLANTAKKPRMSAMYFAAPPLEAKISPLPKMVSPEN 300
Query: 301 -SLYKEFTWFEYKRSAYNSRLADNRLVHFERI 324
Y FTW +YK++ Y+ RL RL F+ +
Sbjct: 301 PRRYNSFTWGDYKKATYSLRLDVPRLEFFKTL 329
BLAST of Sed0020249 vs. TAIR 10
Match:
AT1G47990.1 (gibberellin 2-oxidase 4 )
HSP 1 Score: 277.3 bits (708), Expect = 1.5e-74
Identity = 154/308 (50.00%), Postives = 196/308 (63.64%), Query Frame = 0
Query: 24 VPLIDLS--APDAKHLIVKACEELGFFKVVNHGVPMEFISALESESTKFFSLPLSEKHRA 83
+P+ID+S IVKACE LGFFKV+NHGV IS +E ES FF+ P EK
Sbjct: 15 IPIIDMSQERSQVSMQIVKACESLGFFKVINHGVDQTTISRMEQESINFFAKPAHEKKSV 74
Query: 84 GPAS-PFGYGNKQIGRNGDVGWVEYLLLQTNSHGFLSTFAQHPQKLRSALNNYISSVRNM 143
P + PF YG + IG NGD G VEYLL TN F S + SA+N YI +V+ +
Sbjct: 75 RPVNQPFRYGFRDIGLNGDSGEVEYLLFHTNDPAFRSQLS-----FSSAVNCYIEAVKQL 134
Query: 144 ACKTLELMAEGLKIQQRNAFSKLLMDEESDSVFRVNHYPPCPE---EVRALE---GKNMI 203
A + L+L AEGL + ++FS+L+ +SDSV RVNHYPP + E + +
Sbjct: 135 AREILDLTAEGLHVPP-HSFSRLISSVDSDSVLRVNHYPPSDQFFGEANLSDQSVSLTRV 194
Query: 204 GFGEHTDPQIISVLRSNNTSGLQISLADGNWVSVPPDQNSFFINVGDSLQVMTNGRFKSV 263
GFGEHTDPQI++VLRSN GLQ+S +DG WVSV PD ++F +NVGD LQVMTNGRF SV
Sbjct: 195 GFGEHTDPQILTVLRSNGVGGLQVSNSDGMWVSVSPDPSAFCVNVGDLLQVMTNGRFISV 254
Query: 264 KHRVLTNSLKSRISMIYFGGPPLSEKIAPLPSLM--KGEESLYKEFTWFEYKRSAYNSRL 321
+HR LT +SR+S YF GPPL KI PL +++ + LY+ FTW EYK+ AY+ RL
Sbjct: 255 RHRALTYGEESRLSTAYFAGPPLQAKIGPLSAMVMTMNQPRLYQTFTWGEYKKRAYSLRL 314
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
CAA70330.1 | 1.8e-162 | 90.18 | dioxygenase [Marah macrocarpa] | [more] |
XP_023531222.1 | 5.6e-159 | 86.22 | gibberellin 2-beta-dioxygenase 1-like [Cucurbita pepo subsp. pepo] | [more] |
KAG6600483.1 | 1.6e-158 | 85.92 | Gibberellin 2-beta-dioxygenase 1, partial [Cucurbita argyrosperma subsp. sororia... | [more] |
XP_022942236.1 | 4.7e-158 | 85.63 | gibberellin 2-beta-dioxygenase 1-like [Cucurbita moschata] | [more] |
XP_022981800.1 | 6.2e-158 | 85.63 | gibberellin 2-beta-dioxygenase 1-like [Cucurbita maxima] | [more] |
Match Name | E-value | Identity | Description | |
Q9SQ80 | 1.2e-127 | 68.09 | Gibberellin 2-beta-dioxygenase 1 OS=Pisum sativum OX=3888 GN=GA2OX1 PE=1 SV=1 | [more] |
Q9XG83 | 2.5e-117 | 65.17 | Gibberellin 2-beta-dioxygenase OS=Phaseolus coccineus OX=3886 GN=GA2OX1 PE=2 SV=... | [more] |
Q8LEA2 | 1.1e-104 | 60.18 | Gibberellin 2-beta-dioxygenase 1 OS=Arabidopsis thaliana OX=3702 GN=GA2OX1 PE=2 ... | [more] |
Q8S0S6 | 2.7e-100 | 58.61 | Gibberellin 2-beta-dioxygenase 3 OS=Oryza sativa subsp. japonica OX=39947 GN=GA2... | [more] |
Q9XFR9 | 7.9e-100 | 59.74 | Gibberellin 2-beta-dioxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=GA2OX2 PE=2 ... | [more] |
Match Name | E-value | Identity | Description | |
O04162 | 8.9e-163 | 90.18 | Dioxygenase OS=Marah macrocarpa OX=48242 PE=2 SV=1 | [more] |
A0A6J1FNB4 | 2.3e-158 | 85.63 | gibberellin 2-beta-dioxygenase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111447... | [more] |
A0A6J1J0N2 | 3.0e-158 | 85.63 | gibberellin 2-beta-dioxygenase 1-like OS=Cucurbita maxima OX=3661 GN=LOC11148083... | [more] |
A0A1S3BRQ7 | 4.8e-156 | 84.84 | gibberellin 2-beta-dioxygenase 1 OS=Cucumis melo OX=3656 GN=LOC103492969 PE=3 SV... | [more] |
A0A0A0KXI6 | 3.1e-155 | 84.55 | Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G... | [more] |