Sed0017818 (gene) Chayote v1

Overview
NameSed0017818
Typegene
OrganismSechium edule (Chayote v1)
DescriptionPectinesterase
LocationLG01: 66293453 .. 66296428 (+)
RNA-Seq ExpressionSed0017818
SyntenySed0017818
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CATAAACATGGCTCAAAAGCTTCATTTTTGCATCATTTTCATATCTCTTTCCATTTTATCAACAATCTCCCCATCTTTCTCAGCTCCAAACGCTGTAGATATATGGTGCAACCAAACCCCATATCCCGACGTTTGTAAGCACTTCTTCAAAGGCAAATTCGACCCGAAAAAGCTCTCCGATTTCAAGAAATCGGGGCTCAAAATCGCCATGGAAAGAGCTCTCACAGCCGAGACGCGAACCAAAGAGCACGGCCGAAAATGCCGAAGCCATAAGGAGAAAGCTGCATGGACGGATTGCATGGAGCTTTACCAAAACATAATCCTCCAACTCAACCAAACCGTCTCCGACCAATGCTCCGATTTCGACATCCAAACGTGGCTCAGCTCCGCGTTGACTAACCTCGAGACATGTCGGACCGGGTTCGTCGACCTTAATATCAAGGACTACGACATCAATTACCCGTTCTTGGCGAGTAGTAATGTGAGCAAGCTTATTAGTAATAGTTTGGCAATGAATAACTGCTCGGGGACGGAGACGGGATACCATGAGGGGTTTCCGAGGTGGGTTAACGCGGGAGCGCGGAGGCTGCTGCAGGCGGCTTCACCAAAGGCGGACTTGGTGGTGGCGTTGGATGGATCGGGGAATTATCGGACCGTGCAGGCGGCGGTGGACGCAGCGGGGAAGAGGAAAGGGAGTGGGAGGTTTGTGATATATGTGAAGAGAGGGGTTTATAAGGAGAATGTTGTGATTGGGAATAAAGTGAAGAATTTGATGCTTGTTGGAGATGGAATGAAGTATACTATTATTACTGGGAGTAGAAGTGTTGGTGGTGGTTCTACTACTTTTAACTCTGCTACTGTTGGTGAGTTTCTTTTTTCTCTTCAATTTCTCTTTTTAACATCAAAAAACTGGATTTGAATTCTGTTTTTGGTTCTAAATTTTTTAGACTAGTTAAGATATATCCTAAGCTTTAAAAAATAGGGATTTTTTTTATCATGGTTATGAATTTGCTACCTAATATTTGTATATCTATTAATTTACCTGTCTAGATATGTTTGATATAAATATACATGCTTCAAGTTGTGCTATTTATTTGTTAAAGTAGTAAAATATTTTGCAAGCATGGAAATGGACCAAAGTTGATGTATGAGAATATCTAGACATTTTTTTAACTCAAATGACTATATAATTAGAATAAATTTGAAAATAAAACAACATGAGTGTATGTAGATGATAAAAAAAAACCATGAATTCAAATCTTTCCATTGTTATGAACTAAAAAAAATTGGGAAATAGAGATACAACTTTTTTTTTATCCCTCCATCATGATAAAATATTTTTTCAAGAAAATAAAACATTTTAATGCAAATGATTTTTGTAAAAAAAATGAAAATATTTGGAATTTAAATTTATAAATCATGAACATTTTCAAGATTTTTTTTAATTAGTTGTCACTTTTTTCTTGAAAAAAAAAACAATTTATAGAATTAAAGCCCGTTTGATACCCATTTTGTTTTTCGTTTTTTATTTTTGAAATTTAAGCCTACAACAACTCTTCTACCATAGCTTTTTTCTTATTTTGATATATACATCTTACCTATATTTTAACAATCAAAAGCGAAATTTATAAACAAAAAAAAGTAGCTTCCAAAAACTTGTTTTTGTTTTTGAAATGTGGTTAGTATACACCTACTTTCTTAAGGAAAGAGTGATATATTATAGGAAAGATGGAGCACGTGGTAAGAAATTTGAGTAGAAATAAACTTAAATTTCATAAACAAAAAACTAAAAACGAAATGGTTATCAAACGGGGTCTAAGTGTTATATTGTTAATCCTCATGGCCTATTGGAAAGTATATAAGGTATTGAATATTTCATAATTTCCCCTTCATTTTAGCAAGCACTTGGAAGCCATAATTAAGACATGATGTATGACTTAACAACCATAAATTTATATATTTAAATCTTTATCCCTAAATGTGGATGAGTTAAAAAAAAAACATTGAGACTTGGTAATTTGTTTGCTTTGCTTAGCCGTAACGGGAGAAGGCTTCATCGCCCGTGGCATCACATTCCGCAACACAGCCGGACCCCAAAACCACCAGGCAGTAGCCCTCCGCTCCGGTTCCGATCTCTCCGTCTTCTACCGCTGCGGTTTCGAAGGATATCAAGACACCCTCTACGTCCACTCTCAAAGACAATTCTACCGAGAATGCTACATCTACGGTACCGTCGACTTCATCTTCGGCAACGCAGCCGTGGTTCTCCAAAACTGCATGATCTACGCAAGAAAACCAATGAAAGGGCAGCAATGCGTGGTCACAGCGCAAGGCCGAACGGACCCCAACCAGAACACCGGAATCTCGATCCACAACTCGAGAGTGATGGCCTCGGACGACTTGAAACCGGCGATAAGCTCGGTGAGAACCTACTTGGGGAGGCCGTGGAAGGAGTACTCTCGCACGGTTTACTTGCAAACGTTCATGGATAGCTTAGTGAACCCTGCAGGGTGGTTGGAATGGAGTGGTAATTTTGCTCTTAATACATTGTATTATGGAGAGTTTAAGAACTTTGGGCCTGGTTCTTCTACTGCTAAGAGAGTTAAATGGAAGGGTTATAGAGTAATTACAAGTGTGACAGAAGCTTCTAAGTTTACTGTTGGGAATTTTGTTGCTGGGAGTTCTTGGTTGCCTGCCACTGGTGTTCCATTCAATGCTGGGCTTTAAAGCATATGAATTTGTGGTTGTTTGATGGGTGAATTGGGATTGTATTTTATTGTATTCATCAATAATATATATCATTCTTGGGGAAGATATTCAAATGTTGAATGTTTACTCTCTTTGGTTGAGCCTAAACAAATGTTATATATTGTTGTTATGCTAGAATGAGCAAGTGTGAATAAAATCATCTATCAAATTGAACAAATTAATTGACGGATGGAGAGGGCAATTCTATATAGTTGATGTACAAGAGAG

mRNA sequence

CATAAACATGGCTCAAAAGCTTCATTTTTGCATCATTTTCATATCTCTTTCCATTTTATCAACAATCTCCCCATCTTTCTCAGCTCCAAACGCTGTAGATATATGGTGCAACCAAACCCCATATCCCGACGTTTGTAAGCACTTCTTCAAAGGCAAATTCGACCCGAAAAAGCTCTCCGATTTCAAGAAATCGGGGCTCAAAATCGCCATGGAAAGAGCTCTCACAGCCGAGACGCGAACCAAAGAGCACGGCCGAAAATGCCGAAGCCATAAGGAGAAAGCTGCATGGACGGATTGCATGGAGCTTTACCAAAACATAATCCTCCAACTCAACCAAACCGTCTCCGACCAATGCTCCGATTTCGACATCCAAACGTGGCTCAGCTCCGCGTTGACTAACCTCGAGACATGTCGGACCGGGTTCGTCGACCTTAATATCAAGGACTACGACATCAATTACCCGTTCTTGGCGAGTAGTAATGTGAGCAAGCTTATTAGTAATAGTTTGGCAATGAATAACTGCTCGGGGACGGAGACGGGATACCATGAGGGGTTTCCGAGGTGGGTTAACGCGGGAGCGCGGAGGCTGCTGCAGGCGGCTTCACCAAAGGCGGACTTGGTGGTGGCGTTGGATGGATCGGGGAATTATCGGACCGTGCAGGCGGCGGTGGACGCAGCGGGGAAGAGGAAAGGGAGTGGGAGGTTTGTGATATATGTGAAGAGAGGGGTTTATAAGGAGAATGTTGTGATTGGGAATAAAGTGAAGAATTTGATGCTTGTTGGAGATGGAATGAAGTATACTATTATTACTGGGAGTAGAAGTGTTGGTGGTGGTTCTACTACTTTTAACTCTGCTACTGTTGCCGTAACGGGAGAAGGCTTCATCGCCCGTGGCATCACATTCCGCAACACAGCCGGACCCCAAAACCACCAGGCAGTAGCCCTCCGCTCCGGTTCCGATCTCTCCGTCTTCTACCGCTGCGGTTTCGAAGGATATCAAGACACCCTCTACGTCCACTCTCAAAGACAATTCTACCGAGAATGCTACATCTACGGTACCGTCGACTTCATCTTCGGCAACGCAGCCGTGGTTCTCCAAAACTGCATGATCTACGCAAGAAAACCAATGAAAGGGCAGCAATGCGTGGTCACAGCGCAAGGCCGAACGGACCCCAACCAGAACACCGGAATCTCGATCCACAACTCGAGAGTGATGGCCTCGGACGACTTGAAACCGGCGATAAGCTCGGTGAGAACCTACTTGGGGAGGCCGTGGAAGGAGTACTCTCGCACGGTTTACTTGCAAACGTTCATGGATAGCTTAGTGAACCCTGCAGGGTGGTTGGAATGGAGTGGTAATTTTGCTCTTAATACATTGTATTATGGAGAGTTTAAGAACTTTGGGCCTGGTTCTTCTACTGCTAAGAGAGTTAAATGGAAGGGTTATAGAGTAATTACAAGTGTGACAGAAGCTTCTAAGTTTACTGTTGGGAATTTTGTTGCTGGGAGTTCTTGGTTGCCTGCCACTGGTGTTCCATTCAATGCTGGGCTTTAAAGCATATGAATTTGTGGTTGTTTGATGGGTGAATTGGGATTGTATTTTATTGTATTCATCAATAATATATATCATTCTTGGGGAAGATATTCAAATGTTGAATGTTTACTCTCTTTGGTTGAGCCTAAACAAATGTTATATATTGTTGTTATGCTAGAATGAGCAAGTGTGAATAAAATCATCTATCAAATTGAACAAATTAATTGACGGATGGAGAGGGCAATTCTATATAGTTGATGTACAAGAGAG

Coding sequence (CDS)

ATGGCTCAAAAGCTTCATTTTTGCATCATTTTCATATCTCTTTCCATTTTATCAACAATCTCCCCATCTTTCTCAGCTCCAAACGCTGTAGATATATGGTGCAACCAAACCCCATATCCCGACGTTTGTAAGCACTTCTTCAAAGGCAAATTCGACCCGAAAAAGCTCTCCGATTTCAAGAAATCGGGGCTCAAAATCGCCATGGAAAGAGCTCTCACAGCCGAGACGCGAACCAAAGAGCACGGCCGAAAATGCCGAAGCCATAAGGAGAAAGCTGCATGGACGGATTGCATGGAGCTTTACCAAAACATAATCCTCCAACTCAACCAAACCGTCTCCGACCAATGCTCCGATTTCGACATCCAAACGTGGCTCAGCTCCGCGTTGACTAACCTCGAGACATGTCGGACCGGGTTCGTCGACCTTAATATCAAGGACTACGACATCAATTACCCGTTCTTGGCGAGTAGTAATGTGAGCAAGCTTATTAGTAATAGTTTGGCAATGAATAACTGCTCGGGGACGGAGACGGGATACCATGAGGGGTTTCCGAGGTGGGTTAACGCGGGAGCGCGGAGGCTGCTGCAGGCGGCTTCACCAAAGGCGGACTTGGTGGTGGCGTTGGATGGATCGGGGAATTATCGGACCGTGCAGGCGGCGGTGGACGCAGCGGGGAAGAGGAAAGGGAGTGGGAGGTTTGTGATATATGTGAAGAGAGGGGTTTATAAGGAGAATGTTGTGATTGGGAATAAAGTGAAGAATTTGATGCTTGTTGGAGATGGAATGAAGTATACTATTATTACTGGGAGTAGAAGTGTTGGTGGTGGTTCTACTACTTTTAACTCTGCTACTGTTGCCGTAACGGGAGAAGGCTTCATCGCCCGTGGCATCACATTCCGCAACACAGCCGGACCCCAAAACCACCAGGCAGTAGCCCTCCGCTCCGGTTCCGATCTCTCCGTCTTCTACCGCTGCGGTTTCGAAGGATATCAAGACACCCTCTACGTCCACTCTCAAAGACAATTCTACCGAGAATGCTACATCTACGGTACCGTCGACTTCATCTTCGGCAACGCAGCCGTGGTTCTCCAAAACTGCATGATCTACGCAAGAAAACCAATGAAAGGGCAGCAATGCGTGGTCACAGCGCAAGGCCGAACGGACCCCAACCAGAACACCGGAATCTCGATCCACAACTCGAGAGTGATGGCCTCGGACGACTTGAAACCGGCGATAAGCTCGGTGAGAACCTACTTGGGGAGGCCGTGGAAGGAGTACTCTCGCACGGTTTACTTGCAAACGTTCATGGATAGCTTAGTGAACCCTGCAGGGTGGTTGGAATGGAGTGGTAATTTTGCTCTTAATACATTGTATTATGGAGAGTTTAAGAACTTTGGGCCTGGTTCTTCTACTGCTAAGAGAGTTAAATGGAAGGGTTATAGAGTAATTACAAGTGTGACAGAAGCTTCTAAGTTTACTGTTGGGAATTTTGTTGCTGGGAGTTCTTGGTTGCCTGCCACTGGTGTTCCATTCAATGCTGGGCTTTAA

Protein sequence

MAQKLHFCIIFISLSILSTISPSFSAPNAVDIWCNQTPYPDVCKHFFKGKFDPKKLSDFKKSGLKIAMERALTAETRTKEHGRKCRSHKEKAAWTDCMELYQNIILQLNQTVSDQCSDFDIQTWLSSALTNLETCRTGFVDLNIKDYDINYPFLASSNVSKLISNSLAMNNCSGTETGYHEGFPRWVNAGARRLLQAASPKADLVVALDGSGNYRTVQAAVDAAGKRKGSGRFVIYVKRGVYKENVVIGNKVKNLMLVGDGMKYTIITGSRSVGGGSTTFNSATVAVTGEGFIARGITFRNTAGPQNHQAVALRSGSDLSVFYRCGFEGYQDTLYVHSQRQFYRECYIYGTVDFIFGNAAVVLQNCMIYARKPMKGQQCVVTAQGRTDPNQNTGISIHNSRVMASDDLKPAISSVRTYLGRPWKEYSRTVYLQTFMDSLVNPAGWLEWSGNFALNTLYYGEFKNFGPGSSTAKRVKWKGYRVITSVTEASKFTVGNFVAGSSWLPATGVPFNAGL
Homology
BLAST of Sed0017818 vs. NCBI nr
Match: XP_022996028.1 (pectinesterase-like [Cucurbita maxima])

HSP 1 Score: 901.0 bits (2327), Expect = 4.8e-258
Identity = 443/517 (85.69%), Postives = 475/517 (91.88%), Query Frame = 0

Query: 1   MAQKLHFCIIFISLSILSTISPSFSAPNAVDIWCNQTPYPDVCKHFFKGKFDPKKLSDFK 60
           MAQ LHF I+ IS +IL +ISPSFSAPNA D+WC+QTPYPDVCK FFK KF+PK L++FK
Sbjct: 1   MAQNLHFPILVISFTILLSISPSFSAPNAKDLWCSQTPYPDVCKSFFKAKFNPKNLAEFK 60

Query: 61  KSGLKIAMERALTAETRTKEHGRKCRSHKEKAAWTDCMELYQNIILQLNQTVSDQ-CSDF 120
           K+  KIAMERALTAET TK H +KCR+ KEK AW DC+ELYQ+ IL LN+T SD  CSDF
Sbjct: 61  KTAFKIAMERALTAETLTKRHAQKCRTPKEKGAWADCLELYQDTILHLNRTFSDNYCSDF 120

Query: 121 DIQTWLSSALTNLETCRTGFVDLNIKDYDINYPFLA-SSNVSKLISNSLAMNNCSGTETG 180
           DIQTWLSSALTNLETCR GFVDLNIKDYD+ +PFL+ S+NV+KLI NSLAMNNCS TETG
Sbjct: 121 DIQTWLSSALTNLETCRAGFVDLNIKDYDVVFPFLSRSNNVTKLIRNSLAMNNCSETETG 180

Query: 181 YHEGFPRWVNAGARRLLQAASPKADLVVALDGSGNYRTVQAAVDAAGKRKGSGRFVIYVK 240
           YH GFP WV AG RRLLQAA PKADLVVALDGSGNY+TVQAAVDAA KRKGSGRFVI+VK
Sbjct: 181 YHGGFPTWVKAGERRLLQAAVPKADLVVALDGSGNYKTVQAAVDAAAKRKGSGRFVIHVK 240

Query: 241 RGVYKENVVIGNKVKNLMLVGDGMKYTIITGSRSVGGGSTTFNSATVAVTGEGFIARGIT 300
           +GVYKENVVIGNKVKNLMLVGDG+KYTIITGSRSVGGGSTTFNSATVAVTGEGFIARGIT
Sbjct: 241 KGVYKENVVIGNKVKNLMLVGDGLKYTIITGSRSVGGGSTTFNSATVAVTGEGFIARGIT 300

Query: 301 FRNTAGPQNHQAVALRSGSDLSVFYRCGFEGYQDTLYVHSQRQFYRECYIYGTVDFIFGN 360
           FRNTAGP+NHQAVALRSGSDLSVFYRC FEGYQDTLYVHSQRQFYRECYIYGTVDFIFGN
Sbjct: 301 FRNTAGPENHQAVALRSGSDLSVFYRCSFEGYQDTLYVHSQRQFYRECYIYGTVDFIFGN 360

Query: 361 AAVVLQNCMIYARKPMKGQQCVVTAQGRTDPNQNTGISIHNSRVMASDDLKPAISSVRTY 420
           AAVVLQNCMIYARKPM GQQCVVTAQGR+DPNQNTGISIHNSRVMA+ DL+P +SSVRTY
Sbjct: 361 AAVVLQNCMIYARKPMSGQQCVVTAQGRSDPNQNTGISIHNSRVMATADLQPVLSSVRTY 420

Query: 421 LGRPWKEYSRTVYLQTFMDSLVNPAGWLEWSGNFALNTLYYGEFKNFGPGSSTAKRVKWK 480
           LGRPW+EYSRTVYLQ FMDSLVNPAGWLEWSG+FALNTLYYGEFKNFGPGSSTAKRVKWK
Sbjct: 421 LGRPWREYSRTVYLQCFMDSLVNPAGWLEWSGDFALNTLYYGEFKNFGPGSSTAKRVKWK 480

Query: 481 GYRVITSVTEASKFTVGNFVAGSSWLPATGVPFNAGL 516
           GYRVITS  EASKFTVG+F+AG SWLPATGVPF AGL
Sbjct: 481 GYRVITSAAEASKFTVGSFIAGKSWLPATGVPFTAGL 517

BLAST of Sed0017818 vs. NCBI nr
Match: XP_023534348.1 (pectinesterase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 897.1 bits (2317), Expect = 7.0e-257
Identity = 440/517 (85.11%), Postives = 473/517 (91.49%), Query Frame = 0

Query: 1   MAQKLHFCIIFISLSILSTISPSFSAPNAVDIWCNQTPYPDVCKHFFKGKFDPKKLSDFK 60
           MAQ  HF I+ IS+SIL +ISPS SAPNA ++WC+QTPYPD+CK FF  KF+PK L++FK
Sbjct: 1   MAQNRHFSILLISMSILLSISPSLSAPNAKELWCSQTPYPDICKSFFNAKFNPKNLAEFK 60

Query: 61  KSGLKIAMERALTAETRTKEHGRKCRSHKEKAAWTDCMELYQNIILQLNQTVSDQ-CSDF 120
           K+  KIAMERALTAE+ TK H +KCR+ KEK AW DC+ELYQ+ ILQLN+T SD  CSDF
Sbjct: 61  KAAFKIAMERALTAESLTKRHAQKCRTPKEKGAWADCLELYQDTILQLNRTFSDNYCSDF 120

Query: 121 DIQTWLSSALTNLETCRTGFVDLNIKDYDINYPFLA-SSNVSKLISNSLAMNNCSGTETG 180
           DIQTWLSSALTNL+TCR GFVDLNIKDYD+ +PFL  S+NV+KLI NSLAMNNCS TETG
Sbjct: 121 DIQTWLSSALTNLDTCRAGFVDLNIKDYDVVFPFLGRSNNVTKLIRNSLAMNNCSETETG 180

Query: 181 YHEGFPRWVNAGARRLLQAASPKADLVVALDGSGNYRTVQAAVDAAGKRKGSGRFVIYVK 240
           YH GFP WV AG RRLLQAA PK DLVVALDGSGNY+TVQAAVDAA KRKGSGRFVI+VK
Sbjct: 181 YHGGFPTWVKAGERRLLQAAVPKPDLVVALDGSGNYKTVQAAVDAAAKRKGSGRFVIHVK 240

Query: 241 RGVYKENVVIGNKVKNLMLVGDGMKYTIITGSRSVGGGSTTFNSATVAVTGEGFIARGIT 300
           +GVYKENVVIGNKVKNLMLVGDG+KYTIITGSRSVGGGSTTFNSATVAVTGEGFIARGIT
Sbjct: 241 KGVYKENVVIGNKVKNLMLVGDGLKYTIITGSRSVGGGSTTFNSATVAVTGEGFIARGIT 300

Query: 301 FRNTAGPQNHQAVALRSGSDLSVFYRCGFEGYQDTLYVHSQRQFYRECYIYGTVDFIFGN 360
           FRNTAGPQNHQAVALRSGSDLSVFYRCGFEGYQDTLYVHSQRQFYRECYIYGTVDFIFGN
Sbjct: 301 FRNTAGPQNHQAVALRSGSDLSVFYRCGFEGYQDTLYVHSQRQFYRECYIYGTVDFIFGN 360

Query: 361 AAVVLQNCMIYARKPMKGQQCVVTAQGRTDPNQNTGISIHNSRVMASDDLKPAISSVRTY 420
           AAVVLQNCMIYARKPM GQQCVVTAQGR+DPNQNTGISIHNSRVMA+ DL+P +SSVRTY
Sbjct: 361 AAVVLQNCMIYARKPMSGQQCVVTAQGRSDPNQNTGISIHNSRVMATADLQPVLSSVRTY 420

Query: 421 LGRPWKEYSRTVYLQTFMDSLVNPAGWLEWSGNFALNTLYYGEFKNFGPGSSTAKRVKWK 480
           LGRPWKEYSRTVYLQ FMDSLVNPAGWLEWSG+FALNTLYYGEFKNFGPGSSTAKRVKWK
Sbjct: 421 LGRPWKEYSRTVYLQCFMDSLVNPAGWLEWSGDFALNTLYYGEFKNFGPGSSTAKRVKWK 480

Query: 481 GYRVITSVTEASKFTVGNFVAGSSWLPATGVPFNAGL 516
           GYRVITS  EASKFTVG+F+AG SWLPATGVPF AGL
Sbjct: 481 GYRVITSAAEASKFTVGSFIAGKSWLPATGVPFTAGL 517

BLAST of Sed0017818 vs. NCBI nr
Match: XP_022957920.1 (pectinesterase-like [Cucurbita moschata])

HSP 1 Score: 896.0 bits (2314), Expect = 1.6e-256
Identity = 441/517 (85.30%), Postives = 472/517 (91.30%), Query Frame = 0

Query: 1   MAQKLHFCIIFISLSILSTISPSFSAPNAVDIWCNQTPYPDVCKHFFKGKFDPKKLSDFK 60
           MAQ  H  I+ IS+SIL  ISPSFSAPNA D+WC+QTPYPDVCK FF  KF+PK L++FK
Sbjct: 1   MAQNRHVSILVISMSILLAISPSFSAPNAKDLWCSQTPYPDVCKSFFNAKFNPKNLAEFK 60

Query: 61  KSGLKIAMERALTAETRTKEHGRKCRSHKEKAAWTDCMELYQNIILQLNQTVSDQ-CSDF 120
           K+  KIAMERALTAE+ TK H +KCR+ KEK AW DC+ELYQ+ ILQLN+T SD  CSDF
Sbjct: 61  KTAFKIAMERALTAESLTKRHAQKCRTPKEKGAWADCLELYQDTILQLNRTFSDNYCSDF 120

Query: 121 DIQTWLSSALTNLETCRTGFVDLNIKDYDINYPFLA-SSNVSKLISNSLAMNNCSGTETG 180
           DIQTWLSSALTNLETCR GFVDLNIKDYD+ +PFL  S+NV+KLI NSLAMNNCS TETG
Sbjct: 121 DIQTWLSSALTNLETCRAGFVDLNIKDYDVVFPFLGRSNNVTKLIRNSLAMNNCSETETG 180

Query: 181 YHEGFPRWVNAGARRLLQAASPKADLVVALDGSGNYRTVQAAVDAAGKRKGSGRFVIYVK 240
           YH GFP WV AG RRLLQAA PKADLVVALDGSGNY+TVQAAVDAA KRKGSGRFVI+VK
Sbjct: 181 YHGGFPTWVKAGERRLLQAAVPKADLVVALDGSGNYKTVQAAVDAAAKRKGSGRFVIHVK 240

Query: 241 RGVYKENVVIGNKVKNLMLVGDGMKYTIITGSRSVGGGSTTFNSATVAVTGEGFIARGIT 300
           +GVYKENVVIGNKVKNLMLVGDG+KYTIITGSRSV GGSTTFNSATVAVTGEGFIARGIT
Sbjct: 241 KGVYKENVVIGNKVKNLMLVGDGLKYTIITGSRSVRGGSTTFNSATVAVTGEGFIARGIT 300

Query: 301 FRNTAGPQNHQAVALRSGSDLSVFYRCGFEGYQDTLYVHSQRQFYRECYIYGTVDFIFGN 360
           FRNTAGPQNHQAVALRSGSDLSVFYRCGFEGYQDTLYVHSQRQFY+ECYIYGTVDFIFGN
Sbjct: 301 FRNTAGPQNHQAVALRSGSDLSVFYRCGFEGYQDTLYVHSQRQFYKECYIYGTVDFIFGN 360

Query: 361 AAVVLQNCMIYARKPMKGQQCVVTAQGRTDPNQNTGISIHNSRVMASDDLKPAISSVRTY 420
           AAVVLQNCMIYARKPM GQQCVVTAQGR+DPNQNTGISIHNSRVMA+ DL+P +SSVRTY
Sbjct: 361 AAVVLQNCMIYARKPMSGQQCVVTAQGRSDPNQNTGISIHNSRVMATADLQPVLSSVRTY 420

Query: 421 LGRPWKEYSRTVYLQTFMDSLVNPAGWLEWSGNFALNTLYYGEFKNFGPGSSTAKRVKWK 480
           LGRPWKEYSRTVY+Q FMDSLVNPAGWLEWSG+FALNTLYYGEFKNFGPGSSTAKRVKWK
Sbjct: 421 LGRPWKEYSRTVYMQCFMDSLVNPAGWLEWSGDFALNTLYYGEFKNFGPGSSTAKRVKWK 480

Query: 481 GYRVITSVTEASKFTVGNFVAGSSWLPATGVPFNAGL 516
           GYRVITS  EASKFTVG+F+AG SWLPATGVPF AGL
Sbjct: 481 GYRVITSAAEASKFTVGSFIAGKSWLPATGVPFTAGL 517

BLAST of Sed0017818 vs. NCBI nr
Match: KAG6605649.1 (Pectinesterase 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 887.9 bits (2293), Expect = 4.2e-254
Identity = 435/504 (86.31%), Postives = 464/504 (92.06%), Query Frame = 0

Query: 14  LSILSTISPSFSAPNAVDIWCNQTPYPDVCKHFFKGKFDPKKLSDFKKSGLKIAMERALT 73
           +SIL  ISPSFSAPNA D+WC+QTPYPDVCK FF  KF+PK L++FKK+  KIAMERALT
Sbjct: 1   MSILLAISPSFSAPNAKDLWCSQTPYPDVCKSFFNAKFNPKNLAEFKKTAFKIAMERALT 60

Query: 74  AETRTKEHGRKCRSHKEKAAWTDCMELYQNIILQLNQTVSDQ-CSDFDIQTWLSSALTNL 133
           AE+ TK H +KCR+ KEK AW DC+ELYQ+ ILQLN+T SD  CSDFDIQTWLSSALTNL
Sbjct: 61  AESLTKRHAQKCRTPKEKGAWADCLELYQDTILQLNRTFSDNYCSDFDIQTWLSSALTNL 120

Query: 134 ETCRTGFVDLNIKDYDINYPFLA-SSNVSKLISNSLAMNNCSGTETGYHEGFPRWVNAGA 193
           ETCR GFVDLNIKDYD+ +PFL  S+NV+KLI NSLAMNNCS TETGYH GFP WV AG 
Sbjct: 121 ETCRAGFVDLNIKDYDVVFPFLGRSNNVTKLIRNSLAMNNCSETETGYHGGFPTWVKAGE 180

Query: 194 RRLLQAASPKADLVVALDGSGNYRTVQAAVDAAGKRKGSGRFVIYVKRGVYKENVVIGNK 253
           RRLLQAA PKADLVVALDGSGNY+TVQAAVDAA KRKGSGRFVI+VK+GVYKENVVIGNK
Sbjct: 181 RRLLQAAVPKADLVVALDGSGNYKTVQAAVDAAAKRKGSGRFVIHVKKGVYKENVVIGNK 240

Query: 254 VKNLMLVGDGMKYTIITGSRSVGGGSTTFNSATVAVTGEGFIARGITFRNTAGPQNHQAV 313
           VKNLMLVGDG+KYTIITGSRSVGGGSTTFNSATVAVTGEGFIARGITFRNTAGPQNHQAV
Sbjct: 241 VKNLMLVGDGLKYTIITGSRSVGGGSTTFNSATVAVTGEGFIARGITFRNTAGPQNHQAV 300

Query: 314 ALRSGSDLSVFYRCGFEGYQDTLYVHSQRQFYRECYIYGTVDFIFGNAAVVLQNCMIYAR 373
           ALRSGSDLSVFYRCGFEGYQDTLYVHSQRQFYRECYIYGTVDFIFGNAAVVLQNCMIYAR
Sbjct: 301 ALRSGSDLSVFYRCGFEGYQDTLYVHSQRQFYRECYIYGTVDFIFGNAAVVLQNCMIYAR 360

Query: 374 KPMKGQQCVVTAQGRTDPNQNTGISIHNSRVMASDDLKPAISSVRTYLGRPWKEYSRTVY 433
           KPM GQQC VTAQGR+DPNQNTGISIHNSRVMA+ DL+P +SSVRTYLGRPWKEYSRTVY
Sbjct: 361 KPMSGQQCAVTAQGRSDPNQNTGISIHNSRVMATADLQPVLSSVRTYLGRPWKEYSRTVY 420

Query: 434 LQTFMDSLVNPAGWLEWSGNFALNTLYYGEFKNFGPGSSTAKRVKWKGYRVITSVTEASK 493
           +Q FMDSLVNPAGWLEWSG+FALNTLYYGEFKNFGPGSSTAKRVKWKGYRVITS  EASK
Sbjct: 421 MQCFMDSLVNPAGWLEWSGDFALNTLYYGEFKNFGPGSSTAKRVKWKGYRVITSAAEASK 480

Query: 494 FTVGNFVAGSSWLPATGVPFNAGL 516
           FTVG+F+AG SWLPATGVPF AGL
Sbjct: 481 FTVGSFIAGKSWLPATGVPFTAGL 504

BLAST of Sed0017818 vs. NCBI nr
Match: XP_038875884.1 (pectinesterase 2-like [Benincasa hispida])

HSP 1 Score: 882.9 bits (2280), Expect = 1.4e-252
Identity = 436/521 (83.69%), Postives = 474/521 (90.98%), Query Frame = 0

Query: 1   MAQKLHFCIIFISLSILSTISPSFSAP---NAVDIWCNQTPYPDVCKHFFKGKFDPKKLS 60
           MAQKLHF II ISL ILS+IS  FS     NA+D+WC++ PYPDVCKHFF G+FDPKKLS
Sbjct: 1   MAQKLHFSIILISLFILSSISLCFSTKPNNNAIDLWCSRMPYPDVCKHFFNGQFDPKKLS 60

Query: 61  DFKKSGLKIAMERALTAETRTKEHGRKCRSHKEKAAWTDCMELYQNIILQLNQTVSDQ-C 120
           +FKK+ LKIAM+RA+ AET TKE G+KCR+ KE+AAW DC+ELYQ  IL LN+T SD+ C
Sbjct: 61  EFKKTALKIAMDRAIRAETLTKELGKKCRNEKERAAWADCLELYQTTILHLNKTFSDEKC 120

Query: 121 SDFDIQTWLSSALTNLETCRTGFVDLNIKDYDINYPFLASSNVSKLISNSLAMNNCSGTE 180
           SDFDIQTWLSSALT+LETCR GF+DL IKDYD+ +PFLA++NV+KLISNSLAMNNCS ++
Sbjct: 121 SDFDIQTWLSSALTDLETCRAGFIDLKIKDYDVVFPFLANNNVTKLISNSLAMNNCSESD 180

Query: 181 TGY--HEGFPRWVNAGARRLLQAASPKADLVVALDGSGNYRTVQAAVDAAGKRKGSGRFV 240
            GY  +EGFP+WV  G RRLLQAA PKADLVVA DGSGNYRTVQAAVDAAGKRKGSGRFV
Sbjct: 181 VGYKSNEGFPKWVRRGDRRLLQAAEPKADLVVAQDGSGNYRTVQAAVDAAGKRKGSGRFV 240

Query: 241 IYVKRGVYKENVVIGNKVKNLMLVGDGMKYTIITGSRSVGGGSTTFNSATVAVTGEGFIA 300
           I++KRGVYKENVVI  KVKNLMLVGDG++YTIITGSRSVGGGSTTFNSATVAVTGE FIA
Sbjct: 241 IHIKRGVYKENVVI--KVKNLMLVGDGLRYTIITGSRSVGGGSTTFNSATVAVTGERFIA 300

Query: 301 RGITFRNTAGPQNHQAVALRSGSDLSVFYRCGFEGYQDTLYVHSQRQFYRECYIYGTVDF 360
           RGITFRNTAGP+NHQAVALRSGSDLSVF+RCGFEGYQDTLY HSQRQFYRECYIYGTVDF
Sbjct: 301 RGITFRNTAGPENHQAVALRSGSDLSVFFRCGFEGYQDTLYAHSQRQFYRECYIYGTVDF 360

Query: 361 IFGNAAVVLQNCMIYARKPMKGQQCVVTAQGRTDPNQNTGISIHNSRVMASDDLKPAISS 420
           IFGNAAVVLQNCMIYARKP+ GQQCVVTAQGRTDPNQNTGISIHNSRVMA+DDLKP + S
Sbjct: 361 IFGNAAVVLQNCMIYARKPIPGQQCVVTAQGRTDPNQNTGISIHNSRVMAADDLKPVVGS 420

Query: 421 VRTYLGRPWKEYSRTVYLQTFMDSLVNPAGWLEWSGNFALNTLYYGEFKNFGPGSSTAKR 480
           VRTYLGRPWKEYSRTVYLQ FMD+LVNPAGWLEWSGNFALNTLYYGEFKNFGPGSSTAKR
Sbjct: 421 VRTYLGRPWKEYSRTVYLQNFMDNLVNPAGWLEWSGNFALNTLYYGEFKNFGPGSSTAKR 480

Query: 481 VKWKGYRVITSVTEASKFTVGNFVAGSSWLPATGVPFNAGL 516
           VKWKGYRVITS TEASKFTVGNF+AG SWLP TGVPF AGL
Sbjct: 481 VKWKGYRVITSATEASKFTVGNFIAGRSWLPGTGVPFTAGL 519

BLAST of Sed0017818 vs. ExPASy Swiss-Prot
Match: O04887 (Pectinesterase 2 OS=Citrus sinensis OX=2711 GN=PECS-2.1 PE=2 SV=1)

HSP 1 Score: 627.9 bits (1618), Expect = 1.0e-178
Identity = 322/513 (62.77%), Postives = 392/513 (76.41%), Query Frame = 0

Query: 9   IIFISLSILSTISPSFS-APNAVDIWCNQTPYPDVCKHFFKGKFDP---KKLSDFKKSGL 68
           +I +SL + S    SF  +P  V  WC +TP P  C++F   K D    K+ +DF K  L
Sbjct: 6   LITVSLVLFSLSHTSFGYSPEEVKSWCGKTPNPQPCEYFLTQKTDVTSIKQDTDFYKISL 65

Query: 69  KIAMERALTAETRTKEHGRKCRSHKEKAAWTDCMELYQNIILQLNQT--VSDQCSDFDIQ 128
           ++A+ERA TA++RT   G KCR+ +EKAAW DC ELY+  +L+LNQT   S  C+  D Q
Sbjct: 66  QLALERATTAQSRTYTLGSKCRNEREKAAWEDCRELYELTVLKLNQTSNSSPGCTKVDKQ 125

Query: 129 TWLSSALTNLETCRTGFVDLNIKDYDINYPFLASSNVSKLISNSLAMNNCSGTETGYHEG 188
           TWLS+ALTNLETCR    DL + +Y +    L S+NV+KLISN+L++N     E  Y +G
Sbjct: 126 TWLSTALTNLETCRASLEDLGVPEYVLP---LLSNNVTKLISNTLSLNKVPYNEPSYKDG 185

Query: 189 FPRWVNAGARRLLQAASPKADLVVALDGSGNYRTVQAAVDAAGKRKGSGRFVIYVKRGVY 248
           FP WV  G R+LLQ  +P+A++VVA DGSGN +T+Q AV AA  R G  R+VIY+K G Y
Sbjct: 186 FPTWVKPGDRKLLQ-TTPRANIVVAQDGSGNVKTIQEAV-AAASRAGGSRYVIYIKAGTY 245

Query: 249 KENVVIGNKVKNLMLVGDGMKYTIITGSRSVGGGSTTFNSATVAVTGEGFIARGITFRNT 308
            EN+ +  K+KN+M VGDG+  TIITGS+SVGGG+TTF SATVAV G+ FIAR IT RNT
Sbjct: 246 NENIEV--KLKNIMFVGDGIGKTIITGSKSVGGGATTFKSATVAVVGDNFIARDITIRNT 305

Query: 309 AGPQNHQAVALRSGSDLSVFYRCGFEGYQDTLYVHSQRQFYRECYIYGTVDFIFGNAAVV 368
           AGP NHQAVALRSGSDLSVFYRC FEGYQDTLYVHSQRQFYREC IYGTVDFIFGNAAVV
Sbjct: 306 AGPNNHQAVALRSGSDLSVFYRCSFEGYQDTLYVHSQRQFYRECDIYGTVDFIFGNAAVV 365

Query: 369 LQNCMIYARKPMKGQQCVVTAQGRTDPNQNTGISIHNSRVMASDDLKPAISSVRTYLGRP 428
           LQNC I+ARKP   +   +TAQGRTDPNQ+TGI IHN RV A+ DLKP  SSV+T+LGRP
Sbjct: 366 LQNCNIFARKP-PNRTNTLTAQGRTDPNQSTGIIIHNCRVTAASDLKPVQSSVKTFLGRP 425

Query: 429 WKEYSRTVYLQTFMDSLVNPAGWLEWSGNFALNTLYYGEFKNFGPGSSTAKRVKWKGYRV 488
           WK+YSRTVY++TF+DSL+NPAGW+EWSG+FALNTLYY E+ N GPGSSTA RVKW+GY V
Sbjct: 426 WKQYSRTVYIKTFLDSLINPAGWMEWSGDFALNTLYYAEYMNTGPGSSTANRVKWRGYHV 485

Query: 489 ITSVTEASKFTVGNFVAGSSWLPATGVPFNAGL 516
           +TS ++ S+FTVGNF+AG+SWLPAT VPF +GL
Sbjct: 486 LTSPSQVSQFTVGNFIAGNSWLPATNVPFTSGL 510

BLAST of Sed0017818 vs. ExPASy Swiss-Prot
Match: O22149 (Probable pectinesterase/pectinesterase inhibitor 17 OS=Arabidopsis thaliana OX=3702 GN=PME17 PE=2 SV=2)

HSP 1 Score: 577.8 bits (1488), Expect = 1.2e-163
Identity = 299/515 (58.06%), Postives = 383/515 (74.37%), Query Frame = 0

Query: 7   FCIIFISLSILSTISPSFSAPNAVDIWCNQTPYPDVCKHFF--KGKFDP-KKLSDFKKSG 66
           + I F+ L IL   + S      V  WC+QTP P  C++F       +P K  S+F K  
Sbjct: 7   YIINFVILCILVASTVSGYNQKDVKAWCSQTPNPKPCEYFLTHNSNNEPIKSESEFLKIS 66

Query: 67  LKIAMERALTAETRTKEHGRKCRSHKEKAAWTDCMELYQNIILQLNQTVSD--QCSDFDI 126
           +K+ ++RA+ A+T     G KCR  +EKAAW DC++LY   + ++N+T+    +CS  D 
Sbjct: 67  MKLVLDRAILAKTHAFTLGPKCRDTREKAAWEDCIKLYDLTVSKINETMDPNVKCSKLDA 126

Query: 127 QTWLSSALTNLETCRTGFVDLNIKDYDINYPFLASSNVSKLISNSLAMNNCSGTET-GYH 186
           QTWLS+ALTNL+TCR GF++L +   DI  P L S+NVS L+ N+LA+N      T    
Sbjct: 127 QTWLSTALTNLDTCRAGFLELGVT--DIVLP-LMSNNVSNLLCNTLAINKVPFNYTPPEK 186

Query: 187 EGFPRWVNAGARRLLQAASPKADLVVALDGSGNYRTVQAAVDAAGKRKGSGRFVIYVKRG 246
           +GFP WV  G R+LLQ+++PK + VVA DGSGN++T++ A+DAA    GSGRFVIYVK+G
Sbjct: 187 DGFPSWVKPGDRKLLQSSTPKDNAVVAKDGSGNFKTIKEAIDAA---SGSGRFVIYVKQG 246

Query: 247 VYKENVVIGNKVKNLMLVGDGMKYTIITGSRSVGGGSTTFNSATVAVTGEGFIARGITFR 306
           VY EN+ I  + KN+ML GDG+  TIITGS+SVGGG+TTFNSATVA  G+GFIARGITFR
Sbjct: 247 VYSENLEI--RKKNVMLRGDGIGKTIITGSKSVGGGTTTFNSATVAAVGDGFIARGITFR 306

Query: 307 NTAGPQNHQAVALRSGSDLSVFYRCGFEGYQDTLYVHSQRQFYRECYIYGTVDFIFGNAA 366
           NTAG  N QAVALRSGSDLSVFY+C FE YQDTLYVHS RQFYR+C +YGTVDFIFGNAA
Sbjct: 307 NTAGASNEQAVALRSGSDLSVFYQCSFEAYQDTLYVHSNRQFYRDCDVYGTVDFIFGNAA 366

Query: 367 VVLQNCMIYARKPMKGQQCVVTAQGRTDPNQNTGISIHNSRVMASDDLKPAISSVRTYLG 426
            VLQNC I+AR+P + +   +TAQGR+DPNQNTGI IHNSRV A+ DL+P + S +TYLG
Sbjct: 367 AVLQNCNIFARRP-RSKTNTITAQGRSDPNQNTGIIIHNSRVTAASDLRPVLGSTKTYLG 426

Query: 427 RPWKEYSRTVYLQTFMDSLVNPAGWLEWSGNFALNTLYYGEFKNFGPGSSTAKRVKWKGY 486
           RPW++YSRTV+++T +DSL++P GWLEW GNFAL TL+Y EF+N GPG+ST+ RV W G+
Sbjct: 427 RPWRQYSRTVFMKTSLDSLIDPRGWLEWDGNFALKTLFYAEFQNTGPGASTSGRVTWPGF 486

Query: 487 RVITSVTEASKFTVGNFVAGSSWLPATGVPFNAGL 516
           RV+ S +EASKFTVG F+AG SW+P++ VPF +GL
Sbjct: 487 RVLGSASEASKFTVGTFLAGGSWIPSS-VPFTSGL 511

BLAST of Sed0017818 vs. ExPASy Swiss-Prot
Match: Q9FHN5 (Probable pectinesterase/pectinesterase inhibitor 59 OS=Arabidopsis thaliana OX=3702 GN=PME59 PE=2 SV=1)

HSP 1 Score: 565.8 bits (1457), Expect = 4.8e-160
Identity = 290/538 (53.90%), Postives = 381/538 (70.82%), Query Frame = 0

Query: 1   MAQKLHFCIIFISLSILSTISP--SFSAPNAVDIWCNQTPYPDVCKHFFK---GKFDPKK 60
           M QKL    + + L +L  + P  + +  N+ D WC++TPYPD CK +FK   G   P +
Sbjct: 4   MMQKLSILFLHLILLVLLCVHPLTTVADRNSTD-WCDKTPYPDPCKCYFKNHNGFQQPTQ 63

Query: 61  LSDFKKSGLKIAMERALTAETRTKEHGRKCRSHKEKAAWTDCMELYQNIILQLNQTV--- 120
           LS+F+   ++ AM+RA++A       G+ C   K++A   DC++LY + I+QLN+T+   
Sbjct: 64  LSEFRVMLVEAAMDRAISARAELTNSGKNCTDSKKQAVLADCIDLYGDTIMQLNRTLHGV 123

Query: 121 ------SDQCSDFDIQTWLSSALTNLETCRTGFVDLNIKDYDINYPFLASSNVSKLISNS 180
                 +  C+DFD QTWLS+ALTN ETCR G  DLN+ D+    P ++++ +S LISN 
Sbjct: 124 SPKAGAAKSCTDFDAQTWLSTALTNTETCRRGSSDLNVTDFIT--PIVSNTKISHLISNC 183

Query: 181 LAMNNC------SGTETGYHEGFPRWVNAGARRLLQAASPKADLVVALDGSGNYRTVQAA 240
           LA+N         G  T   +GFP W++   +RLL+A   +A+LVVA DGSG++ TVQAA
Sbjct: 184 LAVNGALLTAGNKGNTTANQKGFPTWLSRKDKRLLRAV--RANLVVAKDGSGHFNTVQAA 243

Query: 241 VDAAGKRK-GSGRFVIYVKRGVYKENVVIGNKVKNLMLVGDGMKYTIITGSRSVGGGSTT 300
           +D AG+RK  SGRFVIYVKRG+Y+EN+ +     ++MLVGDGM+ TIITG RSV GG TT
Sbjct: 244 IDVAGRRKVTSGRFVIYVKRGIYQENINVRLNNDDIMLVGDGMRSTIITGGRSVQGGYTT 303

Query: 301 FNSATVAVTGEGFIARGITFRNTAGPQNHQAVALRSGSDLSVFYRCGFEGYQDTLYVHSQ 360
           +NSAT  + G  FIA+GITFRNTAGP   QAVALRS SDLS+FY+C  EGYQDTL VHSQ
Sbjct: 304 YNSATAGIEGLHFIAKGITFRNTAGPAKGQAVALRSSSDLSIFYKCSIEGYQDTLMVHSQ 363

Query: 361 RQFYRECYIYGTVDFIFGNAAVVLQNCMIYARKPMKGQQCVVTAQGRTDPNQNTGISIHN 420
           RQFYRECYIYGTVDFIFGNAA V QNC+I  R+P+KGQ  V+TAQGR DP QNTGISIHN
Sbjct: 364 RQFYRECYIYGTVDFIFGNAAAVFQNCLILPRRPLKGQANVITAQGRADPFQNTGISIHN 423

Query: 421 SRVMASDDLKPAISSVRTYLGRPWKEYSRTVYLQTFMDSLVNPAGWLEW--SGNFALNTL 480
           SR++ + DLKP + +V+TY+GRPW ++SRTV LQT++D++V+P GW  W     F L+TL
Sbjct: 424 SRILPAPDLKPVVGTVKTYMGRPWMKFSRTVVLQTYLDNVVSPVGWSPWIEGSVFGLDTL 483

Query: 481 YYGEFKNFGPGSSTAKRVKWKGYRVITSVTEASKFTVGNFVAGSSWLPATGVPFNAGL 516
           +Y E+KN GP SST  RV WKG+ V+   ++AS FTVG F+AG++WLP TG+PF +GL
Sbjct: 484 FYAEYKNTGPASSTRWRVSWKGFHVLGRASDASAFTVGKFIAGTAWLPRTGIPFTSGL 536

BLAST of Sed0017818 vs. ExPASy Swiss-Prot
Match: Q9FHN4 (Probable pectinesterase/pectinesterase inhibitor 60 OS=Arabidopsis thaliana OX=3702 GN=PME60 PE=2 SV=1)

HSP 1 Score: 556.2 bits (1432), Expect = 3.8e-157
Identity = 284/538 (52.79%), Postives = 380/538 (70.63%), Query Frame = 0

Query: 1   MAQKLHFCIIFISLSILSTISPSFSAPNA----VDIWCNQTPYPDVCKHFF---KGKFDP 60
           M Q + F  + + L +L  + P  +  +     +D WC++TPYP  CK +F    G   P
Sbjct: 5   MVQNISFLSLHLLLILLLCLRPLTTVADGNSTNIDGWCDKTPYPYPCKRYFIKHSGFRLP 64

Query: 61  KKLSDFKKSGLKIAMERALTAETRTKEHGRKCRSHKEKAAWTDCMELYQNIILQLNQTVS 120
            ++S+F+   ++ AM+RA++A  +     + C   K++A   DC+ LY + ++QLN+T+ 
Sbjct: 65  TQISEFRVLLVEAAMDRAVSAWDKLTNSSKNCTDFKKQAVLADCINLYGDTVMQLNRTLQ 124

Query: 121 D-------QCSDFDIQTWLSSALTNLETCRTGFVDLNIKDYDINYPFLASSNVSKLISNS 180
                   +C+DFD QTWLS+ALTN ETCR G  DLN+ D+    P ++++ +S LISN 
Sbjct: 125 GVSSKTGRRCTDFDAQTWLSTALTNTETCRRGSSDLNVSDF--TTPIVSNTKISHLISNC 184

Query: 181 LAMNNC------SGTETGYHEGFPRWVNAGARRLLQAASPKADLVVALDGSGNYRTVQAA 240
           LA+N        + + TG  +GFP WV+   RRLLQ  S +A+LVVA DGSG+++TVQAA
Sbjct: 185 LAVNGALLTAGKNDSTTGDSKGFPTWVSRKERRLLQLQSVRANLVVAKDGSGHFKTVQAA 244

Query: 241 VDAAGKRK-GSGRFVIYVKRGVYKENVVIGNKVKNLMLVGDGMKYTIITGSRSVGGGSTT 300
           +D AG+RK  SGRFVIYVKRG+Y+EN+ +     N+MLVGDGM+YTIITG RSV GG TT
Sbjct: 245 IDVAGRRKVTSGRFVIYVKRGIYQENLNVRLNNDNIMLVGDGMRYTIITGGRSVKGGYTT 304

Query: 301 FNSATVAVTGEGFIARGITFRNTAGPQNHQAVALRSGSDLSVFYRCGFEGYQDTLYVHSQ 360
           ++SAT  + G  FIA+GI F+NTAGP   QAVALRS SDLS+FYRC  EGYQDTL VHSQ
Sbjct: 305 YSSATAGIEGLHFIAKGIAFQNTAGPAKGQAVALRSSSDLSIFYRCSIEGYQDTLMVHSQ 364

Query: 361 RQFYRECYIYGTVDFIFGNAAVVLQNCMIYARKPMKGQQCVVTAQGRTDPNQNTGISIHN 420
           RQFYRECYIYGTVDFIFGNAAVV QNC+I  R P+KGQ  V+TAQGRTD  QNTGISIHN
Sbjct: 365 RQFYRECYIYGTVDFIFGNAAVVFQNCIILPRLPLKGQANVITAQGRTDLFQNTGISIHN 424

Query: 421 SRVMASDDLKPAISSVRTYLGRPWKEYSRTVYLQTFMDSLVNPAGWLEWS--GNFALNTL 480
           S ++ + DLKP + SV+TY+GRPW  YSRTV L+T++DS+V+P GW  W+    + L+TL
Sbjct: 425 SIIIPAPDLKPVVRSVKTYMGRPWMMYSRTVVLKTYIDSVVSPVGWSPWTKGSTYGLDTL 484

Query: 481 YYGEFKNFGPGSSTAKRVKWKGYRVITSVTEASKFTVGNFVAGSSWLPATGVPFNAGL 516
           +Y E+KN GP SST  RV+WKG+ V++  ++AS F+VG F+AG++WLP +G+PF + L
Sbjct: 485 FYAEYKNIGPASSTRWRVRWKGFHVLSKASDASAFSVGKFIAGTAWLPGSGIPFTSEL 540

BLAST of Sed0017818 vs. ExPASy Swiss-Prot
Match: Q9STY3 (Probable pectinesterase/pectinesterase inhibitor 33 OS=Arabidopsis thaliana OX=3702 GN=PME33 PE=2 SV=1)

HSP 1 Score: 536.6 bits (1381), Expect = 3.1e-151
Identity = 268/510 (52.55%), Postives = 363/510 (71.18%), Query Frame = 0

Query: 33  WCNQTPYPDVCKHFFKGKFD-------PKKLSDFKKSGLKIAMERALTAETRTKEHGRKC 92
           WCN+TP+ + C ++F+           P+  S+F +  +K+A+++A+   ++T + G  C
Sbjct: 87  WCNKTPHAETCNYYFRKSSQNNINLRPPRFRSEFLRMLVKVALDQAVITHSQTVKFGPSC 146

Query: 93  RSHKEKAAWTDCMELYQNIILQLNQTV--------SD-QCSDFDIQTWLSSALTNLETCR 152
            +++ KAAW+DC+ L+QN + QLN+T+        SD +C+DFD QTWLS+A TN+ETCR
Sbjct: 147 TNNQRKAAWSDCVNLFQNTVAQLNRTLKGLNPAASSDVKCTDFDAQTWLSTAQTNIETCR 206

Query: 153 TGFVDLNIKDYDINYPFLASSNVSKLISNSLAMNNC------SGTETGYHEGFPRWVNAG 212
           +G  DLN+ D+ +  P +++ N+S LI N LA+N          T   + E FP WV+  
Sbjct: 207 SGSEDLNVSDFVM--PVISNKNLSDLIGNCLAVNGVLMKQHDHTTTANHKEYFPSWVSRH 266

Query: 213 ARRLLQAAS---PKADLVVALDGSGNYRTVQAAVDAAGKRKGSGRFVIYVKRGVYKENVV 272
            RRLL +AS       LVVA D SG++R++QAA++ A +R+   RFVIYVK+GVY+EN+ 
Sbjct: 267 ERRLLVSASLAKSSPHLVVAQDRSGHFRSIQAAINFAARRRFKSRFVIYVKKGVYRENID 326

Query: 273 IGNKVKNLMLVGDGMKYTIITGSRSVGGGSTTFNSATVAVTGEGFIARGITFRNTAGPQN 332
           +GN   N+MLVGDG + TIIT  RSV  G TT+NSAT    G+ F+A+ +TF NTAGP  
Sbjct: 327 VGNDNHNIMLVGDGERKTIITSGRSVQHGYTTYNSATGGFGGQRFVAKDMTFINTAGPLR 386

Query: 333 HQAVALRSGSDLSVFYRCGFEGYQDTLYVHSQRQFYRECYIYGTVDFIFGNAAVVLQNCM 392
            QAVA+RS SDLSVFYR G  G+QDTLY+HSQRQF+RECYI GT+DFIFGNAAVV QNCM
Sbjct: 387 GQAVAVRSSSDLSVFYRVGIHGFQDTLYIHSQRQFFRECYISGTIDFIFGNAAVVFQNCM 446

Query: 393 IYARKPMKGQQCVVTAQGRTDPNQNTGISIHNSRVMASDDLKPAISSVRTYLGRPWKEYS 452
           I  R+P+ GQ  ++TAQGR DP QNTGI+IH+SR++A+ DLKP I + +TYLGRPW+ YS
Sbjct: 447 ILVRRPLHGQANIITAQGRGDPFQNTGITIHSSRIIAASDLKPVIRAYKTYLGRPWQAYS 506

Query: 453 RTVYLQTFMDSLVNPAGWLEW--SGNFALNTLYYGEFKNFGPGSSTAKRVKWKGYRVITS 512
           R   ++T++D+ ++P GW  W    NFALNT++YGE+KNFGPGSST  RV+WKG+  ITS
Sbjct: 507 RVTIMKTYIDNSISPLGWSPWLRGSNFALNTVFYGEYKNFGPGSSTRWRVRWKGFHAITS 566

Query: 513 VTEASKFTVGNFVAGSSWLPATGVPFNAGL 516
              AS+FTVG+ +AG SWLPATGVPF +GL
Sbjct: 567 TAVASRFTVGSLIAGGSWLPATGVPFKSGL 594

BLAST of Sed0017818 vs. ExPASy TrEMBL
Match: A0A6J1K9P3 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111491356 PE=3 SV=1)

HSP 1 Score: 901.0 bits (2327), Expect = 2.3e-258
Identity = 443/517 (85.69%), Postives = 475/517 (91.88%), Query Frame = 0

Query: 1   MAQKLHFCIIFISLSILSTISPSFSAPNAVDIWCNQTPYPDVCKHFFKGKFDPKKLSDFK 60
           MAQ LHF I+ IS +IL +ISPSFSAPNA D+WC+QTPYPDVCK FFK KF+PK L++FK
Sbjct: 1   MAQNLHFPILVISFTILLSISPSFSAPNAKDLWCSQTPYPDVCKSFFKAKFNPKNLAEFK 60

Query: 61  KSGLKIAMERALTAETRTKEHGRKCRSHKEKAAWTDCMELYQNIILQLNQTVSDQ-CSDF 120
           K+  KIAMERALTAET TK H +KCR+ KEK AW DC+ELYQ+ IL LN+T SD  CSDF
Sbjct: 61  KTAFKIAMERALTAETLTKRHAQKCRTPKEKGAWADCLELYQDTILHLNRTFSDNYCSDF 120

Query: 121 DIQTWLSSALTNLETCRTGFVDLNIKDYDINYPFLA-SSNVSKLISNSLAMNNCSGTETG 180
           DIQTWLSSALTNLETCR GFVDLNIKDYD+ +PFL+ S+NV+KLI NSLAMNNCS TETG
Sbjct: 121 DIQTWLSSALTNLETCRAGFVDLNIKDYDVVFPFLSRSNNVTKLIRNSLAMNNCSETETG 180

Query: 181 YHEGFPRWVNAGARRLLQAASPKADLVVALDGSGNYRTVQAAVDAAGKRKGSGRFVIYVK 240
           YH GFP WV AG RRLLQAA PKADLVVALDGSGNY+TVQAAVDAA KRKGSGRFVI+VK
Sbjct: 181 YHGGFPTWVKAGERRLLQAAVPKADLVVALDGSGNYKTVQAAVDAAAKRKGSGRFVIHVK 240

Query: 241 RGVYKENVVIGNKVKNLMLVGDGMKYTIITGSRSVGGGSTTFNSATVAVTGEGFIARGIT 300
           +GVYKENVVIGNKVKNLMLVGDG+KYTIITGSRSVGGGSTTFNSATVAVTGEGFIARGIT
Sbjct: 241 KGVYKENVVIGNKVKNLMLVGDGLKYTIITGSRSVGGGSTTFNSATVAVTGEGFIARGIT 300

Query: 301 FRNTAGPQNHQAVALRSGSDLSVFYRCGFEGYQDTLYVHSQRQFYRECYIYGTVDFIFGN 360
           FRNTAGP+NHQAVALRSGSDLSVFYRC FEGYQDTLYVHSQRQFYRECYIYGTVDFIFGN
Sbjct: 301 FRNTAGPENHQAVALRSGSDLSVFYRCSFEGYQDTLYVHSQRQFYRECYIYGTVDFIFGN 360

Query: 361 AAVVLQNCMIYARKPMKGQQCVVTAQGRTDPNQNTGISIHNSRVMASDDLKPAISSVRTY 420
           AAVVLQNCMIYARKPM GQQCVVTAQGR+DPNQNTGISIHNSRVMA+ DL+P +SSVRTY
Sbjct: 361 AAVVLQNCMIYARKPMSGQQCVVTAQGRSDPNQNTGISIHNSRVMATADLQPVLSSVRTY 420

Query: 421 LGRPWKEYSRTVYLQTFMDSLVNPAGWLEWSGNFALNTLYYGEFKNFGPGSSTAKRVKWK 480
           LGRPW+EYSRTVYLQ FMDSLVNPAGWLEWSG+FALNTLYYGEFKNFGPGSSTAKRVKWK
Sbjct: 421 LGRPWREYSRTVYLQCFMDSLVNPAGWLEWSGDFALNTLYYGEFKNFGPGSSTAKRVKWK 480

Query: 481 GYRVITSVTEASKFTVGNFVAGSSWLPATGVPFNAGL 516
           GYRVITS  EASKFTVG+F+AG SWLPATGVPF AGL
Sbjct: 481 GYRVITSAAEASKFTVGSFIAGKSWLPATGVPFTAGL 517

BLAST of Sed0017818 vs. ExPASy TrEMBL
Match: A0A6J1H1Z9 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111459307 PE=3 SV=1)

HSP 1 Score: 896.0 bits (2314), Expect = 7.5e-257
Identity = 441/517 (85.30%), Postives = 472/517 (91.30%), Query Frame = 0

Query: 1   MAQKLHFCIIFISLSILSTISPSFSAPNAVDIWCNQTPYPDVCKHFFKGKFDPKKLSDFK 60
           MAQ  H  I+ IS+SIL  ISPSFSAPNA D+WC+QTPYPDVCK FF  KF+PK L++FK
Sbjct: 1   MAQNRHVSILVISMSILLAISPSFSAPNAKDLWCSQTPYPDVCKSFFNAKFNPKNLAEFK 60

Query: 61  KSGLKIAMERALTAETRTKEHGRKCRSHKEKAAWTDCMELYQNIILQLNQTVSDQ-CSDF 120
           K+  KIAMERALTAE+ TK H +KCR+ KEK AW DC+ELYQ+ ILQLN+T SD  CSDF
Sbjct: 61  KTAFKIAMERALTAESLTKRHAQKCRTPKEKGAWADCLELYQDTILQLNRTFSDNYCSDF 120

Query: 121 DIQTWLSSALTNLETCRTGFVDLNIKDYDINYPFLA-SSNVSKLISNSLAMNNCSGTETG 180
           DIQTWLSSALTNLETCR GFVDLNIKDYD+ +PFL  S+NV+KLI NSLAMNNCS TETG
Sbjct: 121 DIQTWLSSALTNLETCRAGFVDLNIKDYDVVFPFLGRSNNVTKLIRNSLAMNNCSETETG 180

Query: 181 YHEGFPRWVNAGARRLLQAASPKADLVVALDGSGNYRTVQAAVDAAGKRKGSGRFVIYVK 240
           YH GFP WV AG RRLLQAA PKADLVVALDGSGNY+TVQAAVDAA KRKGSGRFVI+VK
Sbjct: 181 YHGGFPTWVKAGERRLLQAAVPKADLVVALDGSGNYKTVQAAVDAAAKRKGSGRFVIHVK 240

Query: 241 RGVYKENVVIGNKVKNLMLVGDGMKYTIITGSRSVGGGSTTFNSATVAVTGEGFIARGIT 300
           +GVYKENVVIGNKVKNLMLVGDG+KYTIITGSRSV GGSTTFNSATVAVTGEGFIARGIT
Sbjct: 241 KGVYKENVVIGNKVKNLMLVGDGLKYTIITGSRSVRGGSTTFNSATVAVTGEGFIARGIT 300

Query: 301 FRNTAGPQNHQAVALRSGSDLSVFYRCGFEGYQDTLYVHSQRQFYRECYIYGTVDFIFGN 360
           FRNTAGPQNHQAVALRSGSDLSVFYRCGFEGYQDTLYVHSQRQFY+ECYIYGTVDFIFGN
Sbjct: 301 FRNTAGPQNHQAVALRSGSDLSVFYRCGFEGYQDTLYVHSQRQFYKECYIYGTVDFIFGN 360

Query: 361 AAVVLQNCMIYARKPMKGQQCVVTAQGRTDPNQNTGISIHNSRVMASDDLKPAISSVRTY 420
           AAVVLQNCMIYARKPM GQQCVVTAQGR+DPNQNTGISIHNSRVMA+ DL+P +SSVRTY
Sbjct: 361 AAVVLQNCMIYARKPMSGQQCVVTAQGRSDPNQNTGISIHNSRVMATADLQPVLSSVRTY 420

Query: 421 LGRPWKEYSRTVYLQTFMDSLVNPAGWLEWSGNFALNTLYYGEFKNFGPGSSTAKRVKWK 480
           LGRPWKEYSRTVY+Q FMDSLVNPAGWLEWSG+FALNTLYYGEFKNFGPGSSTAKRVKWK
Sbjct: 421 LGRPWKEYSRTVYMQCFMDSLVNPAGWLEWSGDFALNTLYYGEFKNFGPGSSTAKRVKWK 480

Query: 481 GYRVITSVTEASKFTVGNFVAGSSWLPATGVPFNAGL 516
           GYRVITS  EASKFTVG+F+AG SWLPATGVPF AGL
Sbjct: 481 GYRVITSAAEASKFTVGSFIAGKSWLPATGVPFTAGL 517

BLAST of Sed0017818 vs. ExPASy TrEMBL
Match: A0A1S3ASJ4 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103482536 PE=3 SV=1)

HSP 1 Score: 850.1 bits (2195), Expect = 4.7e-243
Identity = 420/521 (80.61%), Postives = 463/521 (88.87%), Query Frame = 0

Query: 1   MAQKLHFCIIFISLSILSTISPSFSA---PNAVDIWCNQTPYPDVCKHFFK-GKFDPKKL 60
           MAQKLHF I+  SL ILS+ S  FS      A+D+WC++TPYPDVCK+FF  G+FDPK L
Sbjct: 1   MAQKLHFSILLFSLFILSSTSLPFSTKTNKKAIDLWCSRTPYPDVCKNFFNDGQFDPKNL 60

Query: 61  SDFKKSGLKIAMERALTAETRTKEHGRKCRSHKEKAAWTDCMELYQNIILQLNQTVSDQC 120
            D +K+ LKIAMERA+  ET TK  G+KCR+ KE+AAW DC+ELYQ  IL LN+T SD C
Sbjct: 61  LDIQKTALKIAMERAIRTETLTKALGQKCRNKKERAAWADCLELYQTTILHLNKTFSDHC 120

Query: 121 SDFDIQTWLSSALTNLETCRTGFVDLNIKDYDINYPFLASSNVSKLISNSLAMNNCSGTE 180
           S+FDIQTWLSSALTNL+TCR GFVDL IKDY + +PFLA++N++KLISNSLAMNNCS ++
Sbjct: 121 SNFDIQTWLSSALTNLDTCRAGFVDLGIKDYGVVFPFLANNNITKLISNSLAMNNCSESD 180

Query: 181 TG--YHEGFPRWVNAGARRLLQAASPKADLVVALDGSGNYRTVQAAVDAAGKRKGSGRFV 240
            G    EGFP+WV  G RRLLQAA PKADLVVA DGSGNY+TVQAAVDAAGKRKGSGRFV
Sbjct: 181 EGNTSDEGFPKWVRGGDRRLLQAAEPKADLVVAQDGSGNYKTVQAAVDAAGKRKGSGRFV 240

Query: 241 IYVKRGVYKENVVIGNKVKNLMLVGDGMKYTIITGSRSVGGGSTTFNSATVAVTGEGFIA 300
           I +K+GVYKENVVI  KVKNLMLVGDG++YTIITGSRSVGGGSTTFNSATVAVTGE FIA
Sbjct: 241 IRIKKGVYKENVVI--KVKNLMLVGDGLRYTIITGSRSVGGGSTTFNSATVAVTGERFIA 300

Query: 301 RGITFRNTAGPQNHQAVALRSGSDLSVFYRCGFEGYQDTLYVHSQRQFYRECYIYGTVDF 360
           RGITFRNTAGPQNHQAVALRSG+DLSVF+RCGFEGYQDTLY HSQRQFYRECYIYGT+DF
Sbjct: 301 RGITFRNTAGPQNHQAVALRSGADLSVFFRCGFEGYQDTLYAHSQRQFYRECYIYGTIDF 360

Query: 361 IFGNAAVVLQNCMIYARKPMKGQQCVVTAQGRTDPNQNTGISIHNSRVMASDDLKPAISS 420
           IFGNAAVVLQNCMIYARKPM+GQQCVVTAQGRTDPNQNTGISIHNSRVMA+DDLKP I S
Sbjct: 361 IFGNAAVVLQNCMIYARKPMQGQQCVVTAQGRTDPNQNTGISIHNSRVMAADDLKPVIKS 420

Query: 421 VRTYLGRPWKEYSRTVYLQTFMDSLVNPAGWLEWSGNFALNTLYYGEFKNFGPGSSTAKR 480
           VRTYLGRPWKEYSRTVYLQ+FM+SLVNPAGWLEWSGNFALNTLYYGEFKN+GPGSSTA R
Sbjct: 421 VRTYLGRPWKEYSRTVYLQSFMESLVNPAGWLEWSGNFALNTLYYGEFKNYGPGSSTANR 480

Query: 481 VKWKGYRVITSVTEASKFTVGNFVAGSSWLPATGVPFNAGL 516
           VKWKGYRVITS  EA+KFTVGNF+AG+SWLP TGVPF AGL
Sbjct: 481 VKWKGYRVITSANEAAKFTVGNFIAGNSWLPGTGVPFTAGL 519

BLAST of Sed0017818 vs. ExPASy TrEMBL
Match: A0A0A0KPG9 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_5G167190 PE=3 SV=1)

HSP 1 Score: 844.0 bits (2179), Expect = 3.4e-241
Identity = 421/522 (80.65%), Postives = 464/522 (88.89%), Query Frame = 0

Query: 1   MAQKLHFCIIFISLSILSTISPSFSAP---NAVDIWCNQTPYPDVCKHFF-KGKFDPKKL 60
           MAQKLHF II  S+ ILS+ S  FS      A+++WC++TPYPDVCKHFF  G+FDP+ L
Sbjct: 1   MAQKLHFSIILFSMFILSSSSLPFSTKTNNKAIELWCSRTPYPDVCKHFFNNGEFDPRNL 60

Query: 61  SDFKKSGLKIAMERALTAETRTKEHGRKCRSHKEKAAWTDCMELYQNIILQLNQTVSDQ- 120
            D KK+ LKIAMERA+  ET TK  G+KCR+ KE+AAW DC+ELYQ  IL LN+T SD+ 
Sbjct: 61  LDIKKAALKIAMERAMKTETLTKALGQKCRNKKERAAWADCLELYQTTILHLNKTFSDKN 120

Query: 121 CSDFDIQTWLSSALTNLETCRTGFVDLNIKDYDINYPFLASSNVSKLISNSLAMNNCSGT 180
           CS+FDIQTWLSSALTNL TCR GFVDL IKDY + +PFL ++N++KLISNSLAMNNCS +
Sbjct: 121 CSNFDIQTWLSSALTNLHTCRAGFVDLGIKDYGVVFPFLENNNITKLISNSLAMNNCSES 180

Query: 181 ETG--YHEGFPRWVNAGARRLLQAASPKADLVVALDGSGNYRTVQAAVDAAGKRKGSGRF 240
           + G    EGFP+W++ G RRLLQAA PKADLVVA DGSGNY+TVQAAVDAAGKRKGSGRF
Sbjct: 181 DEGNTSDEGFPKWLHGGDRRLLQAAEPKADLVVAQDGSGNYKTVQAAVDAAGKRKGSGRF 240

Query: 241 VIYVKRGVYKENVVIGNKVKNLMLVGDGMKYTIITGSRSVGGGSTTFNSATVAVTGEGFI 300
           VI VK+GVYKENVVI  KVKNLMLVGDG+KYTIITGSRSVGGGSTTFNSATVAVTGE FI
Sbjct: 241 VIRVKKGVYKENVVI--KVKNLMLVGDGLKYTIITGSRSVGGGSTTFNSATVAVTGERFI 300

Query: 301 ARGITFRNTAGPQNHQAVALRSGSDLSVFYRCGFEGYQDTLYVHSQRQFYRECYIYGTVD 360
           ARGITFRNTAGPQNHQAVALRSG+DLSVF+RCGFEGYQDTLYVHSQRQFYRECYIYGTVD
Sbjct: 301 ARGITFRNTAGPQNHQAVALRSGADLSVFFRCGFEGYQDTLYVHSQRQFYRECYIYGTVD 360

Query: 361 FIFGNAAVVLQNCMIYARKPMKGQQCVVTAQGRTDPNQNTGISIHNSRVMASDDLKPAIS 420
           FIFGN+AVVLQNCMIYARKPM+GQQCVVTAQGRTDPNQNTGISIHNSRVMA+DDLKP I 
Sbjct: 361 FIFGNSAVVLQNCMIYARKPMQGQQCVVTAQGRTDPNQNTGISIHNSRVMATDDLKPVIK 420

Query: 421 SVRTYLGRPWKEYSRTVYLQTFMDSLVNPAGWLEWSGNFALNTLYYGEFKNFGPGSSTAK 480
           SVRTYLGRPWKEYSRTVYLQ+FMDSLVNPAGWLEWSGNFALNTLYYGEFKN GPGSSTA 
Sbjct: 421 SVRTYLGRPWKEYSRTVYLQSFMDSLVNPAGWLEWSGNFALNTLYYGEFKNSGPGSSTAN 480

Query: 481 RVKWKGYRVITSVTEASKFTVGNFVAGSSWLPATGVPFNAGL 516
           RVKWKGYRVITS +EA+KFTVG+F+AG+SWLP TGVPF AGL
Sbjct: 481 RVKWKGYRVITSASEAAKFTVGSFIAGNSWLPGTGVPFTAGL 520

BLAST of Sed0017818 vs. ExPASy TrEMBL
Match: A0A5A7ULB7 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold231G00120 PE=3 SV=1)

HSP 1 Score: 772.3 bits (1993), Expect = 1.3e-219
Identity = 378/450 (84.00%), Postives = 411/450 (91.33%), Query Frame = 0

Query: 68  MERALTAETRTKEHGRKCRSHKEKAAWTDCMELYQNIILQLNQTVSDQCSDFDIQTWLSS 127
           MERA+  ET TK  G+KCR+ KE+AAW DC+ELYQ  IL LN+T SD CS+FDIQTWLSS
Sbjct: 1   MERAIRTETLTKALGQKCRNKKERAAWADCLELYQTTILHLNKTFSDHCSNFDIQTWLSS 60

Query: 128 ALTNLETCRTGFVDLNIKDYDINYPFLASSNVSKLISNSLAMNNCSGTETG--YHEGFPR 187
           ALTNL+TCR GFVDL IKDY + +PFLA++N++KLISNSLAMNNCS ++ G    EGFP+
Sbjct: 61  ALTNLDTCRAGFVDLGIKDYGVVFPFLANNNITKLISNSLAMNNCSESDEGNTSDEGFPK 120

Query: 188 WVNAGARRLLQAASPKADLVVALDGSGNYRTVQAAVDAAGKRKGSGRFVIYVKRGVYKEN 247
           WV  G RRLLQAA PKADLVVA DGSGNY+TVQAAVDAAGKRKGSGRFVI +K+GVYKEN
Sbjct: 121 WVRGGDRRLLQAAEPKADLVVAQDGSGNYKTVQAAVDAAGKRKGSGRFVIRIKKGVYKEN 180

Query: 248 VVIGNKVKNLMLVGDGMKYTIITGSRSVGGGSTTFNSATVAVTGEGFIARGITFRNTAGP 307
           VVI  KVKNLMLVGDG++YTIITGSRSVGGGSTTFNSATVAVTGE FIARGITFRNTAGP
Sbjct: 181 VVI--KVKNLMLVGDGLRYTIITGSRSVGGGSTTFNSATVAVTGERFIARGITFRNTAGP 240

Query: 308 QNHQAVALRSGSDLSVFYRCGFEGYQDTLYVHSQRQFYRECYIYGTVDFIFGNAAVVLQN 367
           QNHQAVALRSG+DLSVF+RCGFEGYQDTLY HSQRQFYRECYIYGT+DFIFGNAAVVLQN
Sbjct: 241 QNHQAVALRSGADLSVFFRCGFEGYQDTLYAHSQRQFYRECYIYGTIDFIFGNAAVVLQN 300

Query: 368 CMIYARKPMKGQQCVVTAQGRTDPNQNTGISIHNSRVMASDDLKPAISSVRTYLGRPWKE 427
           CMIYARKPM+GQQCVVTAQGRTDPNQNTGISIHNSRVMA+DDLKP I SVRTYLGRPWKE
Sbjct: 301 CMIYARKPMQGQQCVVTAQGRTDPNQNTGISIHNSRVMAADDLKPVIKSVRTYLGRPWKE 360

Query: 428 YSRTVYLQTFMDSLVNPAGWLEWSGNFALNTLYYGEFKNFGPGSSTAKRVKWKGYRVITS 487
           YSRTVYLQ+FM+SLVNPAGWLEWSGNFALNTLYYGEFKN+GPGSSTA RVKWKGYRVITS
Sbjct: 361 YSRTVYLQSFMESLVNPAGWLEWSGNFALNTLYYGEFKNYGPGSSTANRVKWKGYRVITS 420

Query: 488 VTEASKFTVGNFVAGSSWLPATGVPFNAGL 516
             EA+KFTVGNF+AG+SWLP TGVPF AGL
Sbjct: 421 ANEAAKFTVGNFIAGNSWLPGTGVPFTAGL 448

BLAST of Sed0017818 vs. TAIR 10
Match: AT2G45220.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 577.8 bits (1488), Expect = 8.7e-165
Identity = 299/515 (58.06%), Postives = 383/515 (74.37%), Query Frame = 0

Query: 7   FCIIFISLSILSTISPSFSAPNAVDIWCNQTPYPDVCKHFF--KGKFDP-KKLSDFKKSG 66
           + I F+ L IL   + S      V  WC+QTP P  C++F       +P K  S+F K  
Sbjct: 7   YIINFVILCILVASTVSGYNQKDVKAWCSQTPNPKPCEYFLTHNSNNEPIKSESEFLKIS 66

Query: 67  LKIAMERALTAETRTKEHGRKCRSHKEKAAWTDCMELYQNIILQLNQTVSD--QCSDFDI 126
           +K+ ++RA+ A+T     G KCR  +EKAAW DC++LY   + ++N+T+    +CS  D 
Sbjct: 67  MKLVLDRAILAKTHAFTLGPKCRDTREKAAWEDCIKLYDLTVSKINETMDPNVKCSKLDA 126

Query: 127 QTWLSSALTNLETCRTGFVDLNIKDYDINYPFLASSNVSKLISNSLAMNNCSGTET-GYH 186
           QTWLS+ALTNL+TCR GF++L +   DI  P L S+NVS L+ N+LA+N      T    
Sbjct: 127 QTWLSTALTNLDTCRAGFLELGVT--DIVLP-LMSNNVSNLLCNTLAINKVPFNYTPPEK 186

Query: 187 EGFPRWVNAGARRLLQAASPKADLVVALDGSGNYRTVQAAVDAAGKRKGSGRFVIYVKRG 246
           +GFP WV  G R+LLQ+++PK + VVA DGSGN++T++ A+DAA    GSGRFVIYVK+G
Sbjct: 187 DGFPSWVKPGDRKLLQSSTPKDNAVVAKDGSGNFKTIKEAIDAA---SGSGRFVIYVKQG 246

Query: 247 VYKENVVIGNKVKNLMLVGDGMKYTIITGSRSVGGGSTTFNSATVAVTGEGFIARGITFR 306
           VY EN+ I  + KN+ML GDG+  TIITGS+SVGGG+TTFNSATVA  G+GFIARGITFR
Sbjct: 247 VYSENLEI--RKKNVMLRGDGIGKTIITGSKSVGGGTTTFNSATVAAVGDGFIARGITFR 306

Query: 307 NTAGPQNHQAVALRSGSDLSVFYRCGFEGYQDTLYVHSQRQFYRECYIYGTVDFIFGNAA 366
           NTAG  N QAVALRSGSDLSVFY+C FE YQDTLYVHS RQFYR+C +YGTVDFIFGNAA
Sbjct: 307 NTAGASNEQAVALRSGSDLSVFYQCSFEAYQDTLYVHSNRQFYRDCDVYGTVDFIFGNAA 366

Query: 367 VVLQNCMIYARKPMKGQQCVVTAQGRTDPNQNTGISIHNSRVMASDDLKPAISSVRTYLG 426
            VLQNC I+AR+P + +   +TAQGR+DPNQNTGI IHNSRV A+ DL+P + S +TYLG
Sbjct: 367 AVLQNCNIFARRP-RSKTNTITAQGRSDPNQNTGIIIHNSRVTAASDLRPVLGSTKTYLG 426

Query: 427 RPWKEYSRTVYLQTFMDSLVNPAGWLEWSGNFALNTLYYGEFKNFGPGSSTAKRVKWKGY 486
           RPW++YSRTV+++T +DSL++P GWLEW GNFAL TL+Y EF+N GPG+ST+ RV W G+
Sbjct: 427 RPWRQYSRTVFMKTSLDSLIDPRGWLEWDGNFALKTLFYAEFQNTGPGASTSGRVTWPGF 486

Query: 487 RVITSVTEASKFTVGNFVAGSSWLPATGVPFNAGL 516
           RV+ S +EASKFTVG F+AG SW+P++ VPF +GL
Sbjct: 487 RVLGSASEASKFTVGTFLAGGSWIPSS-VPFTSGL 511

BLAST of Sed0017818 vs. TAIR 10
Match: AT5G51490.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 565.8 bits (1457), Expect = 3.4e-161
Identity = 290/538 (53.90%), Postives = 381/538 (70.82%), Query Frame = 0

Query: 1   MAQKLHFCIIFISLSILSTISP--SFSAPNAVDIWCNQTPYPDVCKHFFK---GKFDPKK 60
           M QKL    + + L +L  + P  + +  N+ D WC++TPYPD CK +FK   G   P +
Sbjct: 4   MMQKLSILFLHLILLVLLCVHPLTTVADRNSTD-WCDKTPYPDPCKCYFKNHNGFQQPTQ 63

Query: 61  LSDFKKSGLKIAMERALTAETRTKEHGRKCRSHKEKAAWTDCMELYQNIILQLNQTV--- 120
           LS+F+   ++ AM+RA++A       G+ C   K++A   DC++LY + I+QLN+T+   
Sbjct: 64  LSEFRVMLVEAAMDRAISARAELTNSGKNCTDSKKQAVLADCIDLYGDTIMQLNRTLHGV 123

Query: 121 ------SDQCSDFDIQTWLSSALTNLETCRTGFVDLNIKDYDINYPFLASSNVSKLISNS 180
                 +  C+DFD QTWLS+ALTN ETCR G  DLN+ D+    P ++++ +S LISN 
Sbjct: 124 SPKAGAAKSCTDFDAQTWLSTALTNTETCRRGSSDLNVTDFIT--PIVSNTKISHLISNC 183

Query: 181 LAMNNC------SGTETGYHEGFPRWVNAGARRLLQAASPKADLVVALDGSGNYRTVQAA 240
           LA+N         G  T   +GFP W++   +RLL+A   +A+LVVA DGSG++ TVQAA
Sbjct: 184 LAVNGALLTAGNKGNTTANQKGFPTWLSRKDKRLLRAV--RANLVVAKDGSGHFNTVQAA 243

Query: 241 VDAAGKRK-GSGRFVIYVKRGVYKENVVIGNKVKNLMLVGDGMKYTIITGSRSVGGGSTT 300
           +D AG+RK  SGRFVIYVKRG+Y+EN+ +     ++MLVGDGM+ TIITG RSV GG TT
Sbjct: 244 IDVAGRRKVTSGRFVIYVKRGIYQENINVRLNNDDIMLVGDGMRSTIITGGRSVQGGYTT 303

Query: 301 FNSATVAVTGEGFIARGITFRNTAGPQNHQAVALRSGSDLSVFYRCGFEGYQDTLYVHSQ 360
           +NSAT  + G  FIA+GITFRNTAGP   QAVALRS SDLS+FY+C  EGYQDTL VHSQ
Sbjct: 304 YNSATAGIEGLHFIAKGITFRNTAGPAKGQAVALRSSSDLSIFYKCSIEGYQDTLMVHSQ 363

Query: 361 RQFYRECYIYGTVDFIFGNAAVVLQNCMIYARKPMKGQQCVVTAQGRTDPNQNTGISIHN 420
           RQFYRECYIYGTVDFIFGNAA V QNC+I  R+P+KGQ  V+TAQGR DP QNTGISIHN
Sbjct: 364 RQFYRECYIYGTVDFIFGNAAAVFQNCLILPRRPLKGQANVITAQGRADPFQNTGISIHN 423

Query: 421 SRVMASDDLKPAISSVRTYLGRPWKEYSRTVYLQTFMDSLVNPAGWLEW--SGNFALNTL 480
           SR++ + DLKP + +V+TY+GRPW ++SRTV LQT++D++V+P GW  W     F L+TL
Sbjct: 424 SRILPAPDLKPVVGTVKTYMGRPWMKFSRTVVLQTYLDNVVSPVGWSPWIEGSVFGLDTL 483

Query: 481 YYGEFKNFGPGSSTAKRVKWKGYRVITSVTEASKFTVGNFVAGSSWLPATGVPFNAGL 516
           +Y E+KN GP SST  RV WKG+ V+   ++AS FTVG F+AG++WLP TG+PF +GL
Sbjct: 484 FYAEYKNTGPASSTRWRVSWKGFHVLGRASDASAFTVGKFIAGTAWLPRTGIPFTSGL 536

BLAST of Sed0017818 vs. TAIR 10
Match: AT5G51500.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 556.2 bits (1432), Expect = 2.7e-158
Identity = 284/538 (52.79%), Postives = 380/538 (70.63%), Query Frame = 0

Query: 1   MAQKLHFCIIFISLSILSTISPSFSAPNA----VDIWCNQTPYPDVCKHFF---KGKFDP 60
           M Q + F  + + L +L  + P  +  +     +D WC++TPYP  CK +F    G   P
Sbjct: 5   MVQNISFLSLHLLLILLLCLRPLTTVADGNSTNIDGWCDKTPYPYPCKRYFIKHSGFRLP 64

Query: 61  KKLSDFKKSGLKIAMERALTAETRTKEHGRKCRSHKEKAAWTDCMELYQNIILQLNQTVS 120
            ++S+F+   ++ AM+RA++A  +     + C   K++A   DC+ LY + ++QLN+T+ 
Sbjct: 65  TQISEFRVLLVEAAMDRAVSAWDKLTNSSKNCTDFKKQAVLADCINLYGDTVMQLNRTLQ 124

Query: 121 D-------QCSDFDIQTWLSSALTNLETCRTGFVDLNIKDYDINYPFLASSNVSKLISNS 180
                   +C+DFD QTWLS+ALTN ETCR G  DLN+ D+    P ++++ +S LISN 
Sbjct: 125 GVSSKTGRRCTDFDAQTWLSTALTNTETCRRGSSDLNVSDF--TTPIVSNTKISHLISNC 184

Query: 181 LAMNNC------SGTETGYHEGFPRWVNAGARRLLQAASPKADLVVALDGSGNYRTVQAA 240
           LA+N        + + TG  +GFP WV+   RRLLQ  S +A+LVVA DGSG+++TVQAA
Sbjct: 185 LAVNGALLTAGKNDSTTGDSKGFPTWVSRKERRLLQLQSVRANLVVAKDGSGHFKTVQAA 244

Query: 241 VDAAGKRK-GSGRFVIYVKRGVYKENVVIGNKVKNLMLVGDGMKYTIITGSRSVGGGSTT 300
           +D AG+RK  SGRFVIYVKRG+Y+EN+ +     N+MLVGDGM+YTIITG RSV GG TT
Sbjct: 245 IDVAGRRKVTSGRFVIYVKRGIYQENLNVRLNNDNIMLVGDGMRYTIITGGRSVKGGYTT 304

Query: 301 FNSATVAVTGEGFIARGITFRNTAGPQNHQAVALRSGSDLSVFYRCGFEGYQDTLYVHSQ 360
           ++SAT  + G  FIA+GI F+NTAGP   QAVALRS SDLS+FYRC  EGYQDTL VHSQ
Sbjct: 305 YSSATAGIEGLHFIAKGIAFQNTAGPAKGQAVALRSSSDLSIFYRCSIEGYQDTLMVHSQ 364

Query: 361 RQFYRECYIYGTVDFIFGNAAVVLQNCMIYARKPMKGQQCVVTAQGRTDPNQNTGISIHN 420
           RQFYRECYIYGTVDFIFGNAAVV QNC+I  R P+KGQ  V+TAQGRTD  QNTGISIHN
Sbjct: 365 RQFYRECYIYGTVDFIFGNAAVVFQNCIILPRLPLKGQANVITAQGRTDLFQNTGISIHN 424

Query: 421 SRVMASDDLKPAISSVRTYLGRPWKEYSRTVYLQTFMDSLVNPAGWLEWS--GNFALNTL 480
           S ++ + DLKP + SV+TY+GRPW  YSRTV L+T++DS+V+P GW  W+    + L+TL
Sbjct: 425 SIIIPAPDLKPVVRSVKTYMGRPWMMYSRTVVLKTYIDSVVSPVGWSPWTKGSTYGLDTL 484

Query: 481 YYGEFKNFGPGSSTAKRVKWKGYRVITSVTEASKFTVGNFVAGSSWLPATGVPFNAGL 516
           +Y E+KN GP SST  RV+WKG+ V++  ++AS F+VG F+AG++WLP +G+PF + L
Sbjct: 485 FYAEYKNIGPASSTRWRVRWKGFHVLSKASDASAFSVGKFIAGTAWLPGSGIPFTSEL 540

BLAST of Sed0017818 vs. TAIR 10
Match: AT3G47400.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 536.6 bits (1381), Expect = 2.2e-152
Identity = 268/510 (52.55%), Postives = 363/510 (71.18%), Query Frame = 0

Query: 33  WCNQTPYPDVCKHFFKGKFD-------PKKLSDFKKSGLKIAMERALTAETRTKEHGRKC 92
           WCN+TP+ + C ++F+           P+  S+F +  +K+A+++A+   ++T + G  C
Sbjct: 87  WCNKTPHAETCNYYFRKSSQNNINLRPPRFRSEFLRMLVKVALDQAVITHSQTVKFGPSC 146

Query: 93  RSHKEKAAWTDCMELYQNIILQLNQTV--------SD-QCSDFDIQTWLSSALTNLETCR 152
            +++ KAAW+DC+ L+QN + QLN+T+        SD +C+DFD QTWLS+A TN+ETCR
Sbjct: 147 TNNQRKAAWSDCVNLFQNTVAQLNRTLKGLNPAASSDVKCTDFDAQTWLSTAQTNIETCR 206

Query: 153 TGFVDLNIKDYDINYPFLASSNVSKLISNSLAMNNC------SGTETGYHEGFPRWVNAG 212
           +G  DLN+ D+ +  P +++ N+S LI N LA+N          T   + E FP WV+  
Sbjct: 207 SGSEDLNVSDFVM--PVISNKNLSDLIGNCLAVNGVLMKQHDHTTTANHKEYFPSWVSRH 266

Query: 213 ARRLLQAAS---PKADLVVALDGSGNYRTVQAAVDAAGKRKGSGRFVIYVKRGVYKENVV 272
            RRLL +AS       LVVA D SG++R++QAA++ A +R+   RFVIYVK+GVY+EN+ 
Sbjct: 267 ERRLLVSASLAKSSPHLVVAQDRSGHFRSIQAAINFAARRRFKSRFVIYVKKGVYRENID 326

Query: 273 IGNKVKNLMLVGDGMKYTIITGSRSVGGGSTTFNSATVAVTGEGFIARGITFRNTAGPQN 332
           +GN   N+MLVGDG + TIIT  RSV  G TT+NSAT    G+ F+A+ +TF NTAGP  
Sbjct: 327 VGNDNHNIMLVGDGERKTIITSGRSVQHGYTTYNSATGGFGGQRFVAKDMTFINTAGPLR 386

Query: 333 HQAVALRSGSDLSVFYRCGFEGYQDTLYVHSQRQFYRECYIYGTVDFIFGNAAVVLQNCM 392
            QAVA+RS SDLSVFYR G  G+QDTLY+HSQRQF+RECYI GT+DFIFGNAAVV QNCM
Sbjct: 387 GQAVAVRSSSDLSVFYRVGIHGFQDTLYIHSQRQFFRECYISGTIDFIFGNAAVVFQNCM 446

Query: 393 IYARKPMKGQQCVVTAQGRTDPNQNTGISIHNSRVMASDDLKPAISSVRTYLGRPWKEYS 452
           I  R+P+ GQ  ++TAQGR DP QNTGI+IH+SR++A+ DLKP I + +TYLGRPW+ YS
Sbjct: 447 ILVRRPLHGQANIITAQGRGDPFQNTGITIHSSRIIAASDLKPVIRAYKTYLGRPWQAYS 506

Query: 453 RTVYLQTFMDSLVNPAGWLEW--SGNFALNTLYYGEFKNFGPGSSTAKRVKWKGYRVITS 512
           R   ++T++D+ ++P GW  W    NFALNT++YGE+KNFGPGSST  RV+WKG+  ITS
Sbjct: 507 RVTIMKTYIDNSISPLGWSPWLRGSNFALNTVFYGEYKNFGPGSSTRWRVRWKGFHAITS 566

Query: 513 VTEASKFTVGNFVAGSSWLPATGVPFNAGL 516
              AS+FTVG+ +AG SWLPATGVPF +GL
Sbjct: 567 TAVASRFTVGSLIAGGSWLPATGVPFKSGL 594

BLAST of Sed0017818 vs. TAIR 10
Match: AT1G23200.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 449.1 bits (1154), Expect = 4.7e-126
Identity = 253/528 (47.92%), Postives = 326/528 (61.74%), Query Frame = 0

Query: 34  CNQTPYPDVCKHFFKGKFDPKKLSDFKKSG-------LKIAMERALTAETRTKEHGRKCR 93
           C QTPYP VC H       P K  D +  G       +   M++A+          +   
Sbjct: 38  CKQTPYPSVCDHHMSN--SPLKTLDDQTDGFTFHDLVVSSTMDQAVQLHRLVSSLKQHHS 97

Query: 94  SHKE-KAAWTDCMELYQNIILQLN---QTVSDQCSDFDIQTWLSSALTNLETCRTGFVDL 153
            HK   +A  DC+ELY++ I QLN   ++     S  D QT LS+A+ N +TCR GF D 
Sbjct: 98  LHKHATSALFDCLELYEDTIDQLNHSRRSYGQYSSPHDRQTSLSAAIANQDTCRNGFRDF 157

Query: 154 NI-KDYDINYPFLASSNVSKLISNSLAMNNCSG---------TETGY------------- 213
            +   Y   +P     N++K ISNSLA+   +            TG+             
Sbjct: 158 KLTSSYSKYFPVQFHRNLTKSISNSLAVTKAAAEAEAVAEKYPSTGFTKFSKQRSSAGGG 217

Query: 214 ---------HEGFPRWVNAGARRLLQ--AASPKADLVVALDGSGNYRTVQAAVDAAGK-R 273
                     E FP W     R+LL+    + KADLVVA DGSG+Y ++Q AV+AA K  
Sbjct: 218 SHRRLLLFSDEKFPSWFPLSDRKLLEDSKTTAKADLVVAKDGSGHYTSIQQAVNAAAKLP 277

Query: 274 KGSGRFVIYVKRGVYKENVVIGNKVKNLMLVGDGMKYTIITGSRSVGGGSTTFNSATVAV 333
           + + R VIYVK GVY+ENVVI   +KN+M++GDG+  TI+TG+R+V  G+TTF SAT AV
Sbjct: 278 RRNQRLVIYVKAGVYRENVVIKKSIKNVMVIGDGIDSTIVTGNRNVQDGTTTFRSATFAV 337

Query: 334 TGEGFIARGITFRNTAGPQNHQAVALRSGSDLSVFYRCGFEGYQDTLYVHSQRQFYRECY 393
           +G GFIA+GITF NTAGP+ HQAVALRS SD SVFY C F+GYQDTLY+HS RQF R C 
Sbjct: 338 SGNGFIAQGITFENTAGPEKHQAVALRSSSDFSVFYACSFKGYQDTLYLHSSRQFLRNCN 397

Query: 394 IYGTVDFIFGNAAVVLQNCMIYARKPMKGQQCVVTAQGRTDPNQNTGISIHNSRVMASDD 453
           IYGTVDFIFG+A  +LQNC IYARKPM GQ+  +TAQ R +P++ TG  I +S V  + +
Sbjct: 398 IYGTVDFIFGDATAILQNCNIYARKPMSGQKNTITAQSRKEPDETTGFVIQSSTVATASE 457

Query: 454 LKPAISSVRTYLGRPWKEYSRTVYLQTFMDSLVNPAGWLEWSGNFALNTLYYGEFKNFGP 513
                    TYLGRPW+ +SRTV+++  + +LV+PAGWL WSG+FAL+TLYYGE+ N G 
Sbjct: 458 ---------TYLGRPWRSHSRTVFMKCNLGALVSPAGWLPWSGSFALSTLYYGEYGNTGA 517

Query: 514 GSSTAKRVKWKGYRVITSVTEASKFTVGNFVAGSSWLPATGVPFNAGL 516
           G+S + RVKW GY VI +VTEA KFTV NF+ G+ W+ ATGVP N GL
Sbjct: 518 GASVSGRVKWPGYHVIKTVTEAEKFTVENFLDGNYWITATGVPVNDGL 554

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022996028.14.8e-25885.69pectinesterase-like [Cucurbita maxima][more]
XP_023534348.17.0e-25785.11pectinesterase-like [Cucurbita pepo subsp. pepo][more]
XP_022957920.11.6e-25685.30pectinesterase-like [Cucurbita moschata][more]
KAG6605649.14.2e-25486.31Pectinesterase 2, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_038875884.11.4e-25283.69pectinesterase 2-like [Benincasa hispida][more]
Match NameE-valueIdentityDescription
O048871.0e-17862.77Pectinesterase 2 OS=Citrus sinensis OX=2711 GN=PECS-2.1 PE=2 SV=1[more]
O221491.2e-16358.06Probable pectinesterase/pectinesterase inhibitor 17 OS=Arabidopsis thaliana OX=3... [more]
Q9FHN54.8e-16053.90Probable pectinesterase/pectinesterase inhibitor 59 OS=Arabidopsis thaliana OX=3... [more]
Q9FHN43.8e-15752.79Probable pectinesterase/pectinesterase inhibitor 60 OS=Arabidopsis thaliana OX=3... [more]
Q9STY33.1e-15152.55Probable pectinesterase/pectinesterase inhibitor 33 OS=Arabidopsis thaliana OX=3... [more]
Match NameE-valueIdentityDescription
A0A6J1K9P32.3e-25885.69Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111491356 PE=3 SV=1[more]
A0A6J1H1Z97.5e-25785.30Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111459307 PE=3 SV=1[more]
A0A1S3ASJ44.7e-24380.61Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103482536 PE=3 SV=1[more]
A0A0A0KPG93.4e-24180.65Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_5G167190 PE=3 SV=1[more]
A0A5A7ULB71.3e-21984.00Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold231G00120... [more]
Match NameE-valueIdentityDescription
AT2G45220.18.7e-16558.06Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT5G51490.13.4e-16153.90Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT5G51500.12.7e-15852.79Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT3G47400.12.2e-15252.55Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT1G23200.14.7e-12647.92Plant invertase/pectin methylesterase inhibitor superfamily [more]
InterPro
Analysis Name: InterPro Annotations of Chayote (edule) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006501Pectinesterase inhibitor domainSMARTSM00856PMEI_2coord: 25..169
e-value: 3.2E-26
score: 103.1
IPR006501Pectinesterase inhibitor domainTIGRFAMTIGR01614TIGR01614coord: 6..171
e-value: 5.4E-18
score: 62.9
IPR006501Pectinesterase inhibitor domainPFAMPF04043PMEIcoord: 29..169
e-value: 5.2E-24
score: 85.3
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 197..515
e-value: 4.8E-127
score: 425.7
IPR000070Pectinesterase, catalyticPFAMPF01095Pectinesterasecoord: 203..500
e-value: 4.7E-145
score: 481.9
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilyGENE3D1.20.140.40Invertase/pectin methylesterase inhibitor family proteincoord: 26..172
e-value: 6.6E-26
score: 93.1
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilySUPERFAMILY101148Plant invertase/pectin methylesterase inhibitorcoord: 28..172
NoneNo IPR availablePANTHERPTHR31707:SF181PECTINESTERASEcoord: 7..514
NoneNo IPR availablePANTHERPTHR31707PECTINESTERASEcoord: 7..514
NoneNo IPR availableCDDcd15798PMEI-like_3coord: 33..171
e-value: 2.94758E-31
score: 116.384
IPR018040Pectinesterase, Tyr active sitePROSITEPS00800PECTINESTERASE_1coord: 229..248
IPR033131Pectinesterase, Asp active sitePROSITEPS00503PECTINESTERASE_2coord: 348..357
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 199..514

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sed0017818.1Sed0017818.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042545 cell wall modification
biological_process GO:0043086 negative regulation of catalytic activity
biological_process GO:0045490 pectin catabolic process
cellular_component GO:0005576 extracellular region
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0045330 aspartyl esterase activity
molecular_function GO:0030599 pectinesterase activity
molecular_function GO:0046910 pectinesterase inhibitor activity
molecular_function GO:0004857 enzyme inhibitor activity