Sed0015954 (gene) Chayote v1

BLAST of Sed0015954 vs. NCBI nr
Match: KAG6590040.1 (Subtilisin-like protease 1.8, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1392.1 bits (3602), Expect = 0.0e+00
Identity = 702/766 (91.64%), Postives = 731/766 (95.43%), Query Frame = 0

Query: 1   MGSMAKFLMAS--LLLLLPCVFINAKKTTYIVHMKHHALPSDYPT-------HLQSLSST 60
           MGSMA+ L+AS  LLLLLPCVF+NAK+ TYIV MKHHALPSDY T       HLQSLSS+
Sbjct: 1   MGSMARLLVASFLLLLLLPCVFVNAKR-TYIVRMKHHALPSDYLTHHDWYSAHLQSLSSS 60

Query: 61  A-SDSLLYTYTSAYHGFAASLDPDEAELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSD 120
           A SDSLLYTYTSAYHGFAASLDPDEAELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSD
Sbjct: 61  ATSDSLLYTYTSAYHGFAASLDPDEAELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSD 120

Query: 121 FGLWEGHNTQDLDQASHDVIIGVLDTGIWPESKSFDDVGMPEIPSRWRGECESGPDFSPS 180
           FGLWEGHNTQDLDQASHDVIIGVLDTGIWPESKSFDD GMPEIPSRWRGECESGPDFSPS
Sbjct: 121 FGLWEGHNTQDLDQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGECESGPDFSPS 180

Query: 181 LCNKKLIGARSFSKGYQMASGGGYFKKPREDQSARDQDGHGTHTASTAAGSHVANASLLG 240
           LCNKKLIGARSFSKGYQMASGGGYF+K RE++S RDQDGHGTHTASTAAGSHVANASLLG
Sbjct: 181 LCNKKLIGARSFSKGYQMASGGGYFRKSRENESPRDQDGHGTHTASTAAGSHVANASLLG 240

Query: 241 YARGIARGMAPQARVAAYKTCWPTGCFGSDILAAMDRAILDGVDVLSLSLGGGSAPYYKD 300
           YARGIARGMAPQARVAAYKTCWPTGCFGSDILA MDRAI DGVDVLSLSLGGGSAPYY+D
Sbjct: 241 YARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRD 300

Query: 301 TIAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVDLGNGRR 360
           TIAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYV LGNG+R
Sbjct: 301 TIAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKR 360

Query: 361 ITGVSLYSGQGMGSKPVGLVYNKGSNSSSNVCLPGSLEPALVRGKVVICDRGVNARVEKG 420
            TGVSLYSGQGMG+KPV LVYNKGSNSSSN+CLPGSLEPA VRGKVVICDRG+NARVEKG
Sbjct: 361 FTGVSLYSGQGMGNKPVALVYNKGSNSSSNMCLPGSLEPAFVRGKVVICDRGINARVEKG 420

Query: 421 GVVRDAGGIGMILANTAASGEELVADSHLLPAVAVGRKVGDLIRQYVRSVSKPMAALSFG 480
           GVVRDAGGIGMILANTAASGEELVADSHLLPAVAVG K GDLIRQYVRSV+KP A LSFG
Sbjct: 421 GVVRDAGGIGMILANTAASGEELVADSHLLPAVAVGAKTGDLIRQYVRSVAKPTAVLSFG 480

Query: 481 GTILNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESVGPTGLDSDKRKTQ 540
           GT+LNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSES+GPTGLD+DKRKTQ
Sbjct: 481 GTVLNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDNDKRKTQ 540

Query: 541 FNIMSGTSMSCPHISGLAALLKAAHSEWSPSAIKSALMTTAYTQDNTNSSLRDAAGGAFS 600
           FNIMSGTSMSCPHISGLAALLKAAH  WSPSAIKSALMTTAYTQDNTNSSLRDAAGGAFS
Sbjct: 541 FNIMSGTSMSCPHISGLAALLKAAHPSWSPSAIKSALMTTAYTQDNTNSSLRDAAGGAFS 600

Query: 601 NPWAHGAGHVDPHKALSPGLVYDISTDDYIDFLCSLDYGIDHVKAIVKRSNITCSRKFAD 660
           NPWAHGAGHVDPHKALSPGLVYDIST+DYI FLCSLDYGIDHV+AI KRSNITC +KFAD
Sbjct: 601 NPWAHGAGHVDPHKALSPGLVYDISTNDYITFLCSLDYGIDHVQAIAKRSNITCLKKFAD 660

Query: 661 PGQLNYPSFSVVFGSKRVVRYTRKVTNVGAAGSVYEVSTTAPSVVNVIVKPSKLVFTKVG 720
           PGQLNYPSFSVVFGSKRVVRYTR VTNVGAAGSVYEV+TTAPSVV V VKPSKLVF+KVG
Sbjct: 661 PGQLNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVYEVATTAPSVVKVTVKPSKLVFSKVG 720

Query: 721 ERKRYTITFVASRDAARTTKFGFGSIAWTNEQHQVKSPVAFAWTRL 757
           ERKRYT+TFVASRDAARTT++GFGSIAW+N+QHQV+SPVAFAWT+L
Sbjct: 721 ERKRYTVTFVASRDAARTTRYGFGSIAWSNDQHQVRSPVAFAWTKL 765

BLAST of Sed0015954 vs. NCBI nr
Match: XP_022960792.1 (subtilisin-like protease SBT1.8 [Cucurbita moschata] >KAG7023704.1 Subtilisin-like protease SBT1.8 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1391.7 bits (3601), Expect = 0.0e+00
Identity = 702/767 (91.53%), Postives = 731/767 (95.31%), Query Frame = 0

Query: 1   MGSMAKFLMAS---LLLLLPCVFINAKKTTYIVHMKHHALPSDYPT-------HLQSLSS 60
           MGSMA+ L+AS   LLLLLPCVF+NAK+ TYIV MKHHALPSDY T       HLQSLSS
Sbjct: 1   MGSMARLLVASFLLLLLLLPCVFVNAKR-TYIVRMKHHALPSDYLTHHDWYSAHLQSLSS 60

Query: 61  TA-SDSLLYTYTSAYHGFAASLDPDEAELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDS 120
           +A SDSLLYTYTSAYHGFAASLDPDEAELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDS
Sbjct: 61  SATSDSLLYTYTSAYHGFAASLDPDEAELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDS 120

Query: 121 DFGLWEGHNTQDLDQASHDVIIGVLDTGIWPESKSFDDVGMPEIPSRWRGECESGPDFSP 180
           DFGLWEGHNTQDLDQASHDVIIGVLDTGIWPESKSFDD GMPEIPSRWRGECESGPDFSP
Sbjct: 121 DFGLWEGHNTQDLDQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGECESGPDFSP 180

Query: 181 SLCNKKLIGARSFSKGYQMASGGGYFKKPREDQSARDQDGHGTHTASTAAGSHVANASLL 240
           SLCNKKLIGARSFSKGYQMASGGGYF+K RE++S RDQDGHGTHTASTAAGSHVANASLL
Sbjct: 181 SLCNKKLIGARSFSKGYQMASGGGYFRKSRENESPRDQDGHGTHTASTAAGSHVANASLL 240

Query: 241 GYARGIARGMAPQARVAAYKTCWPTGCFGSDILAAMDRAILDGVDVLSLSLGGGSAPYYK 300
           GYARGIARGMAPQARVAAYKTCWPTGCFGSDILA MDRAI DGVDVLSLSLGGGSAPYY+
Sbjct: 241 GYARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYR 300

Query: 301 DTIAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVDLGNGR 360
           DTIAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYV LGNG+
Sbjct: 301 DTIAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGK 360

Query: 361 RITGVSLYSGQGMGSKPVGLVYNKGSNSSSNVCLPGSLEPALVRGKVVICDRGVNARVEK 420
           R TGVSLYSGQGMG+KPV LVYNKGSNSSSN+CLPGSLEPA VRGKVVICDRG+NARVEK
Sbjct: 361 RFTGVSLYSGQGMGNKPVALVYNKGSNSSSNMCLPGSLEPAFVRGKVVICDRGINARVEK 420

Query: 421 GGVVRDAGGIGMILANTAASGEELVADSHLLPAVAVGRKVGDLIRQYVRSVSKPMAALSF 480
           GGVVRDAGGIGMILANTAASGEELVADSHLLPAVAVG K GDLIRQYVRSV+KP A LSF
Sbjct: 421 GGVVRDAGGIGMILANTAASGEELVADSHLLPAVAVGAKTGDLIRQYVRSVAKPTAVLSF 480

Query: 481 GGTILNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESVGPTGLDSDKRKT 540
           GGT+LNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSES+GPTGLD+DKRKT
Sbjct: 481 GGTVLNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDNDKRKT 540

Query: 541 QFNIMSGTSMSCPHISGLAALLKAAHSEWSPSAIKSALMTTAYTQDNTNSSLRDAAGGAF 600
           QFNIMSGTSMSCPHISGLAALLKAAH  WSPSAIKSALMTTAYTQDNTNSSLRDAAGGAF
Sbjct: 541 QFNIMSGTSMSCPHISGLAALLKAAHPSWSPSAIKSALMTTAYTQDNTNSSLRDAAGGAF 600

Query: 601 SNPWAHGAGHVDPHKALSPGLVYDISTDDYIDFLCSLDYGIDHVKAIVKRSNITCSRKFA 660
           SNPWAHGAGHVDPHKALSPGLVYDIST+DYI FLCSLDYGIDHV+AI KRSNITC +KFA
Sbjct: 601 SNPWAHGAGHVDPHKALSPGLVYDISTNDYITFLCSLDYGIDHVQAIAKRSNITCLKKFA 660

Query: 661 DPGQLNYPSFSVVFGSKRVVRYTRKVTNVGAAGSVYEVSTTAPSVVNVIVKPSKLVFTKV 720
           DPGQLNYPSFSVVFGSKRVVRYTR VTNVGAAGSVYEV+TTAPSVV V VKPSKLVF+KV
Sbjct: 661 DPGQLNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVYEVATTAPSVVKVTVKPSKLVFSKV 720

Query: 721 GERKRYTITFVASRDAARTTKFGFGSIAWTNEQHQVKSPVAFAWTRL 757
           GERKRYT+TFVASRDAARTT++GFGSIAW+N+QHQV+SPVAFAWT+L
Sbjct: 721 GERKRYTVTFVASRDAARTTRYGFGSIAWSNDQHQVRSPVAFAWTKL 766

BLAST of Sed0015954 vs. NCBI nr
Match: XP_022987083.1 (subtilisin-like protease SBT1.8 [Cucurbita maxima])

HSP 1 Score: 1390.2 bits (3597), Expect = 0.0e+00
Identity = 701/767 (91.40%), Postives = 731/767 (95.31%), Query Frame = 0

Query: 1   MGSMAKFLMAS---LLLLLPCVFINAKKTTYIVHMKHHALPSDYPT-------HLQSLSS 60
           MGSMA+ L+AS   LLLLLPCVF+NAK+ TYIV MKHHALPSDY T       HLQSLSS
Sbjct: 1   MGSMARLLVASFLLLLLLLPCVFVNAKR-TYIVRMKHHALPSDYLTHHDWYSAHLQSLSS 60

Query: 61  TA-SDSLLYTYTSAYHGFAASLDPDEAELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDS 120
           +A SDSLLYTYTSAYHGFAASLDPDEAELLRQSDSVLGVYEDTVY LHTTRTPGFLGLDS
Sbjct: 61  SATSDSLLYTYTSAYHGFAASLDPDEAELLRQSDSVLGVYEDTVYKLHTTRTPGFLGLDS 120

Query: 121 DFGLWEGHNTQDLDQASHDVIIGVLDTGIWPESKSFDDVGMPEIPSRWRGECESGPDFSP 180
           DFGLWEGHNTQDLDQASHDVIIGVLDTGIWPESKSFDD GMPEIPSRWRGECESGPDFSP
Sbjct: 121 DFGLWEGHNTQDLDQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGECESGPDFSP 180

Query: 181 SLCNKKLIGARSFSKGYQMASGGGYFKKPREDQSARDQDGHGTHTASTAAGSHVANASLL 240
           SLCNKKLIGARSFSKGYQMASGGGYF+K RE++S RDQDGHGTHTASTAAGSHVANASLL
Sbjct: 181 SLCNKKLIGARSFSKGYQMASGGGYFRKSRENESPRDQDGHGTHTASTAAGSHVANASLL 240

Query: 241 GYARGIARGMAPQARVAAYKTCWPTGCFGSDILAAMDRAILDGVDVLSLSLGGGSAPYYK 300
           GYARGIARGMAPQARVAAYKTCWPTGCFGSDILA MDRAI DGVDVLSLSLGGGSAPYY+
Sbjct: 241 GYARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYR 300

Query: 301 DTIAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVDLGNGR 360
           DTIAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYV LGNG+
Sbjct: 301 DTIAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGK 360

Query: 361 RITGVSLYSGQGMGSKPVGLVYNKGSNSSSNVCLPGSLEPALVRGKVVICDRGVNARVEK 420
           R TGVSLYSGQGMG+KPV LVYNKGSNSSSN+CLPGSLEPA VRGKVVICDRG+NARVEK
Sbjct: 361 RFTGVSLYSGQGMGNKPVALVYNKGSNSSSNMCLPGSLEPAFVRGKVVICDRGINARVEK 420

Query: 421 GGVVRDAGGIGMILANTAASGEELVADSHLLPAVAVGRKVGDLIRQYVRSVSKPMAALSF 480
           GGVVRDAGGIGMILANTAASGEELVADSHLLPAVAVG K GDLIRQYVRSV+KP A LSF
Sbjct: 421 GGVVRDAGGIGMILANTAASGEELVADSHLLPAVAVGAKTGDLIRQYVRSVAKPTAVLSF 480

Query: 481 GGTILNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESVGPTGLDSDKRKT 540
           GGT+LNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSES+GPTGLD+DKRKT
Sbjct: 481 GGTVLNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDNDKRKT 540

Query: 541 QFNIMSGTSMSCPHISGLAALLKAAHSEWSPSAIKSALMTTAYTQDNTNSSLRDAAGGAF 600
           QFNIMSGTSMSCPHISGLAALLKAAH  WSPSAIKSALMTTAYTQDNTNSSLRDAAGG F
Sbjct: 541 QFNIMSGTSMSCPHISGLAALLKAAHPSWSPSAIKSALMTTAYTQDNTNSSLRDAAGGMF 600

Query: 601 SNPWAHGAGHVDPHKALSPGLVYDISTDDYIDFLCSLDYGIDHVKAIVKRSNITCSRKFA 660
           SNPWAHGAGHVDPHKALSPGL+YDIST+DYI FLCSLDYGIDHV+AIVKRSNITCS+KFA
Sbjct: 601 SNPWAHGAGHVDPHKALSPGLLYDISTNDYITFLCSLDYGIDHVQAIVKRSNITCSKKFA 660

Query: 661 DPGQLNYPSFSVVFGSKRVVRYTRKVTNVGAAGSVYEVSTTAPSVVNVIVKPSKLVFTKV 720
           DPGQLNYPSFSVVFGSKRVVRYTR VTNVGAAGSVYEV+TTAPSVV V VKPSKLVF+KV
Sbjct: 661 DPGQLNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVYEVATTAPSVVKVTVKPSKLVFSKV 720

Query: 721 GERKRYTITFVASRDAARTTKFGFGSIAWTNEQHQVKSPVAFAWTRL 757
           GERKRYT+TFVASRDAARTT++GFGSIAW+N+QHQV+SPVAFAWT+L
Sbjct: 721 GERKRYTVTFVASRDAARTTRYGFGSIAWSNDQHQVRSPVAFAWTKL 766

BLAST of Sed0015954 vs. NCBI nr
Match: XP_023516159.1 (subtilisin-like protease SBT1.8 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1389.4 bits (3595), Expect = 0.0e+00
Identity = 701/766 (91.51%), Postives = 729/766 (95.17%), Query Frame = 0

Query: 1   MGSMAKFLMA--SLLLLLPCVFINAKKTTYIVHMKHHALPSDYPT-------HLQSLSST 60
           MGSMA  L+A   LLLLLPCVF+NAK+ TYIV MKHHALPSDY T       HLQSLSS+
Sbjct: 1   MGSMASLLVAYFLLLLLLPCVFVNAKR-TYIVRMKHHALPSDYLTHHDWYSAHLQSLSSS 60

Query: 61  A-SDSLLYTYTSAYHGFAASLDPDEAELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSD 120
           A SDSLLYTYTSAYHGFAASLDPDEAELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSD
Sbjct: 61  ATSDSLLYTYTSAYHGFAASLDPDEAELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSD 120

Query: 121 FGLWEGHNTQDLDQASHDVIIGVLDTGIWPESKSFDDVGMPEIPSRWRGECESGPDFSPS 180
           FGLWEGHNTQDLDQASHDVIIGVLDTGIWPESKSFDD GMPEIPSRWRGECESGPDFSPS
Sbjct: 121 FGLWEGHNTQDLDQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGECESGPDFSPS 180

Query: 181 LCNKKLIGARSFSKGYQMASGGGYFKKPREDQSARDQDGHGTHTASTAAGSHVANASLLG 240
           LCNKKLIGARSFSKGYQMASGGGYF+K RE++S RDQDGHGTHTASTAAGSHVANASLLG
Sbjct: 181 LCNKKLIGARSFSKGYQMASGGGYFRKSRENESPRDQDGHGTHTASTAAGSHVANASLLG 240

Query: 241 YARGIARGMAPQARVAAYKTCWPTGCFGSDILAAMDRAILDGVDVLSLSLGGGSAPYYKD 300
           YARGIARGMAPQARVAAYKTCWPTGCFGSDILA MDRAI DGVDVLSLSLGGGSAPYY+D
Sbjct: 241 YARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRD 300

Query: 301 TIAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVDLGNGRR 360
           TIAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYV LGNG+R
Sbjct: 301 TIAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKR 360

Query: 361 ITGVSLYSGQGMGSKPVGLVYNKGSNSSSNVCLPGSLEPALVRGKVVICDRGVNARVEKG 420
            TGVSLYSGQGMG+KPV LVYNKGSNSSSN+CLPGSLEPA VRGKVVICDRG+NARVEKG
Sbjct: 361 FTGVSLYSGQGMGNKPVALVYNKGSNSSSNMCLPGSLEPAFVRGKVVICDRGINARVEKG 420

Query: 421 GVVRDAGGIGMILANTAASGEELVADSHLLPAVAVGRKVGDLIRQYVRSVSKPMAALSFG 480
           GVVRDAGGIGMILANTAASGEELVADSHLLPAVAVG K GDLIRQYVRSV+KP A LSFG
Sbjct: 421 GVVRDAGGIGMILANTAASGEELVADSHLLPAVAVGAKTGDLIRQYVRSVAKPTAVLSFG 480

Query: 481 GTILNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESVGPTGLDSDKRKTQ 540
           GT+LNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSES+GPTGLD+DKRKTQ
Sbjct: 481 GTVLNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDNDKRKTQ 540

Query: 541 FNIMSGTSMSCPHISGLAALLKAAHSEWSPSAIKSALMTTAYTQDNTNSSLRDAAGGAFS 600
           FNIMSGTSMSCPHISGLAALLKAAH  WSPSAIKSALMTTAYTQDNTNSSLRDAAGG FS
Sbjct: 541 FNIMSGTSMSCPHISGLAALLKAAHPSWSPSAIKSALMTTAYTQDNTNSSLRDAAGGMFS 600

Query: 601 NPWAHGAGHVDPHKALSPGLVYDISTDDYIDFLCSLDYGIDHVKAIVKRSNITCSRKFAD 660
           NPWAHGAGHVDPHKALSPGLVYDIST+DYI FLCSLDYGIDHV+AI KRSNITCS+KFAD
Sbjct: 601 NPWAHGAGHVDPHKALSPGLVYDISTNDYITFLCSLDYGIDHVQAIAKRSNITCSKKFAD 660

Query: 661 PGQLNYPSFSVVFGSKRVVRYTRKVTNVGAAGSVYEVSTTAPSVVNVIVKPSKLVFTKVG 720
           PGQLNYPSFSVVFGSKRVVRYTR VTNVGAAGSVYEV+TTAPSVV V VKPSKLVF+KVG
Sbjct: 661 PGQLNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVYEVATTAPSVVKVTVKPSKLVFSKVG 720

Query: 721 ERKRYTITFVASRDAARTTKFGFGSIAWTNEQHQVKSPVAFAWTRL 757
           ERKRYT+TFVASRDAARTT++GFGSIAW+N+QHQV+SPVAFAWT+L
Sbjct: 721 ERKRYTVTFVASRDAARTTRYGFGSIAWSNDQHQVRSPVAFAWTKL 765

BLAST of Sed0015954 vs. NCBI nr
Match: XP_038879224.1 (subtilisin-like protease SBT1.8 [Benincasa hispida])

HSP 1 Score: 1375.1 bits (3558), Expect = 0.0e+00
Identity = 694/764 (90.84%), Postives = 726/764 (95.03%), Query Frame = 0

Query: 1   MGSMAKFLMASLLLLLPCVFINAKKTTYIVHMKHHALPSDYPT-------HLQSLSSTA- 60
           MGSMA+ L+A LLLLLPCVF+NAKK TYIV MKH+ALPS+Y T       HLQSLSS++ 
Sbjct: 1   MGSMARLLIAFLLLLLPCVFVNAKK-TYIVRMKHNALPSEYLTHHDWYSAHLQSLSSSST 60

Query: 61  SDSLLYTYTSAYHGFAASLDPDEAELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSDFG 120
           SDSLLYTYTSAYHGFAASLD +EAELLRQSDSVLGVYED+VY LHTTRTPGFLGLDSDFG
Sbjct: 61  SDSLLYTYTSAYHGFAASLDSNEAELLRQSDSVLGVYEDSVYTLHTTRTPGFLGLDSDFG 120

Query: 121 LWEGHNTQDLDQASHDVIIGVLDTGIWPESKSFDDVGMPEIPSRWRGECESGPDFSPSLC 180
           LWEGHNTQDL+QASHDVIIGVLDTGIWPESKSFDD GMPEIPSRWRGECESG DFSPSLC
Sbjct: 121 LWEGHNTQDLNQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGECESGADFSPSLC 180

Query: 181 NKKLIGARSFSKGYQMASGGGYFKKPREDQSARDQDGHGTHTASTAAGSHVANASLLGYA 240
           NKKLIGARSFSKGYQMASGGGYF+KPRE++S RDQDGHGTHTASTAAGSHVANASLLGYA
Sbjct: 181 NKKLIGARSFSKGYQMASGGGYFRKPRENESPRDQDGHGTHTASTAAGSHVANASLLGYA 240

Query: 241 RGIARGMAPQARVAAYKTCWPTGCFGSDILAAMDRAILDGVDVLSLSLGGGSAPYYKDTI 300
           RGIARGMAPQARVAAYKTCWPTGCFGSDILA MDRAI DGVDVLSLSLGGGSAPYY+DTI
Sbjct: 241 RGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRDTI 300

Query: 301 AIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVDLGNGRRIT 360
           AIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYV +GNG+R T
Sbjct: 301 AIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQIGNGKRFT 360

Query: 361 GVSLYSGQGMGSKPVGLVYNKGSNSSSNVCLPGSLEPALVRGKVVICDRGVNARVEKGGV 420
           GVSLYSGQGMGSK V LVYNKGSN+SSN+CLPGSLEPA+VRGKVV+CDRG+NARVEKGGV
Sbjct: 361 GVSLYSGQGMGSKAVELVYNKGSNTSSNMCLPGSLEPAVVRGKVVVCDRGINARVEKGGV 420

Query: 421 VRDAGGIGMILANTAASGEELVADSHLLPAVAVGRKVGDLIRQYVRSVSKPMAALSFGGT 480
           VRDAGGIGMILANTAASGEELVADSHLLPAVAVGRK GDLIRQYVRS S P A LSFGGT
Sbjct: 421 VRDAGGIGMILANTAASGEELVADSHLLPAVAVGRKTGDLIRQYVRSDSNPTAVLSFGGT 480

Query: 481 ILNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESVGPTGLDSDKRKTQFN 540
           ILNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSES+GPTGLD+DKRKTQFN
Sbjct: 481 ILNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDNDKRKTQFN 540

Query: 541 IMSGTSMSCPHISGLAALLKAAHSEWSPSAIKSALMTTAYTQDNTNSSLRDAAGGAFSNP 600
           IMSGTSMSCPHISGLAALLKAAH +WSPSAIKSALMTTAYTQDNTNSSLRDAAGG FSNP
Sbjct: 541 IMSGTSMSCPHISGLAALLKAAHPQWSPSAIKSALMTTAYTQDNTNSSLRDAAGGGFSNP 600

Query: 601 WAHGAGHVDPHKALSPGLVYDISTDDYIDFLCSLDYGIDHVKAIVKRSNITCSRKFADPG 660
           WAHGAGHVDPHKALSPGL+YDIST+DYI FLCSLDYGIDHV+AIVKRSNITCSRKFADPG
Sbjct: 601 WAHGAGHVDPHKALSPGLLYDISTNDYIAFLCSLDYGIDHVQAIVKRSNITCSRKFADPG 660

Query: 661 QLNYPSFSVVFGSKRVVRYTRKVTNVGAAGSVYEVSTTAPSVVNVIVKPSKLVFTKVGER 720
           QLNYPSFSVVFGSKRVVRYTR VTNVGAAGSVYEV+TTAPSVV V VKPSKLVFTKVGER
Sbjct: 661 QLNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVYEVATTAPSVVKVTVKPSKLVFTKVGER 720

Query: 721 KRYTITFVASRDAARTTKFGFGSIAWTNEQHQVKSPVAFAWTRL 757
           KRYT+TFVASRDAA+T +FGFGSIAW N QHQV+SPVAFAWTRL
Sbjct: 721 KRYTVTFVASRDAAQTMRFGFGSIAWNNYQHQVRSPVAFAWTRL 763

BLAST of Sed0015954 vs. ExPASy Swiss-Prot
Match: Q9ZUF6 (Subtilisin-like protease SBT1.8 OS=Arabidopsis thaliana OX=3702 GN=SBT1.8 PE=1 SV=1)

HSP 1 Score: 1073.5 bits (2775), Expect = 7.6e-313
Identity = 530/734 (72.21%), Postives = 620/734 (84.47%), Query Frame = 0

Query: 25  KTTYIVHMKHHALPSDYPTHLQSLSS--TASDSLLYTYTSAYHGFAASLDPDEAE-LLRQ 84
           K TYI+ + H   P  + TH    +S   +  SLLYTYT+++HGF+A LD  EA+ LL  
Sbjct: 27  KKTYIIRVNHSDKPESFLTHHDWYTSQLNSESSLLYTYTTSFHGFSAYLDSTEADSLLSS 86

Query: 85  SDSVLGVYEDTVYNLHTTRTPGFLGLDSDFGLWEGHNTQDLDQASHDVIIGVLDTGIWPE 144
           S+S+L ++ED +Y LHTTRTP FLGL+S+FG+       DL  +S+ VIIGVLDTG+WPE
Sbjct: 87  SNSILDIFEDPLYTLHTTRTPEFLGLNSEFGV------HDLGSSSNGVIIGVLDTGVWPE 146

Query: 145 SKSFDDVGMPEIPSRWRGECESGPDFSPSLCNKKLIGARSFSKGYQMASGGGYFKKPRED 204
           S+SFDD  MPEIPS+W+GECESG DF   LCNKKLIGARSFSKG+QMASGGG+  K RE 
Sbjct: 147 SRSFDDTDMPEIPSKWKGECESGSDFDSKLCNKKLIGARSFSKGFQMASGGGFSSK-RES 206

Query: 205 QSARDQDGHGTHTASTAAGSHVANASLLGYARGIARGMAPQARVAAYKTCWPTGCFGSDI 264
            S RD DGHGTHT++TAAGS V NAS LGYA G ARGMA +ARVA YK CW TGCFGSDI
Sbjct: 207 VSPRDVDGHGTHTSTTAAGSAVRNASFLGYAAGTARGMATRARVATYKVCWSTGCFGSDI 266

Query: 265 LAAMDRAILDGVDVLSLSLGGGSAPYYKDTIAIGAFAAMEKGVFVSCSAGNSGPNKASLA 324
           LAAMDRAILDGVDVLSLSLGGGSAPYY+DTIAIGAF+AME+GVFVSCSAGNSGP +AS+A
Sbjct: 267 LAAMDRAILDGVDVLSLSLGGGSAPYYRDTIAIGAFSAMERGVFVSCSAGNSGPTRASVA 326

Query: 325 NVAPWIMTVGAGTLDRDFPAYVDLGNGRRITGVSLYSGQGMGSKPVGLVYNKGSNSSSNV 384
           NVAPW+MTVGAGTLDRDFPA+ +LGNG+R+TGVSLYSG GMG+KP+ LVYNKG++SSSN+
Sbjct: 327 NVAPWVMTVGAGTLDRDFPAFANLGNGKRLTGVSLYSGVGMGTKPLELVYNKGNSSSSNL 386

Query: 385 CLPGSLEPALVRGKVVICDRGVNARVEKGGVVRDAGGIGMILANTAASGEELVADSHLLP 444
           CLPGSL+ ++VRGK+V+CDRGVNARVEKG VVRDAGG+GMI+ANTAASGEELVADSHLLP
Sbjct: 387 CLPGSLDSSIVRGKIVVCDRGVNARVEKGAVVRDAGGLGMIMANTAASGEELVADSHLLP 446

Query: 445 AVAVGRKVGDLIRQYVRSVSKPMAALSFGGTILNVRPSPVVAAFSSRGPNLVTPQILKPD 504
           A+AVG+K GDL+R+YV+S SKP A L F GT+L+V+PSPVVAAFSSRGPN VTP+ILKPD
Sbjct: 447 AIAVGKKTGDLLREYVKSDSKPTALLVFKGTVLDVKPSPVVAAFSSRGPNTVTPEILKPD 506

Query: 505 VIGPGVNILAAWSESVGPTGLDSDKRKTQFNIMSGTSMSCPHISGLAALLKAAHSEWSPS 564
           VIGPGVNILA WS+++GPTGLD D R+TQFNIMSGTSMSCPHISGLA LLKAAH EWSPS
Sbjct: 507 VIGPGVNILAGWSDAIGPTGLDKDSRRTQFNIMSGTSMSCPHISGLAGLLKAAHPEWSPS 566

Query: 565 AIKSALMTTAYTQDNTNSSLRDAAGGAFSNPWAHGAGHVDPHKALSPGLVYDISTDDYID 624
           AIKSALMTTAY  DNTN+ L DAA  + SNP+AHG+GHVDP KALSPGLVYDIST++YI 
Sbjct: 567 AIKSALMTTAYVLDNTNAPLHDAADNSLSNPYAHGSGHVDPQKALSPGLVYDISTEEYIR 626

Query: 625 FLCSLDYGIDHVKAIVKRSNITCSRKFADPGQLNYPSFSVVFGSKRVVRYTRKVTNVGAA 684
           FLCSLDY +DH+ AIVKR ++ CS+KF+DPGQLNYPSFSV+FG KRVVRYTR+VTNVGAA
Sbjct: 627 FLCSLDYTVDHIVAIVKRPSVNCSKKFSDPGQLNYPSFSVLFGGKRVVRYTREVTNVGAA 686

Query: 685 GSVYEVSTTAPSVVNVIVKPSKLVFTKVGERKRYTITFVASRDAARTTKFGFGSIAWTNE 744
            SVY+V+      V + VKPSKL F  VGE+KRYT+TFV+ +  + T K  FGSI W+N 
Sbjct: 687 SSVYKVTVNGAPSVGISVKPSKLSFKSVGEKKRYTVTFVSKKGVSMTNKAEFGSITWSNP 746

Query: 745 QHQVKSPVAFAWTR 756
           QH+V+SPVAF+W R
Sbjct: 747 QHEVRSPVAFSWNR 753

BLAST of Sed0015954 vs. ExPASy Swiss-Prot
Match: O65351 (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 SV=1)

HSP 1 Score: 781.9 bits (2018), Expect = 6.3e-225
Identity = 416/769 (54.10%), Postives = 535/769 (69.57%), Query Frame = 0

Query: 5   AKFLMASLLLLLPCVFI------NAKKTTYIVHMKHHALPSDYPTH-------LQSLSST 64
           + FL ++   LL C+        ++ + TYIVHM    +PS +  H       L+S+S +
Sbjct: 3   SSFLSSTAFFLLLCLGFCHVSSSSSDQGTYIVHMAKSQMPSSFDLHSNWYDSSLRSISDS 62

Query: 65  ASDSLLYTYTSAYHGFAASLDPDEAELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSDF 124
           A   LLYTY +A HGF+  L  +EA+ L     V+ V  +  Y LHTTRTP FLGLD   
Sbjct: 63  A--ELLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLD--- 122

Query: 125 GLWEGHNTQDL--DQASH-DVIIGVLDTGIWPESKSFDDVGMPEIPSRWRGECESGPDFS 184
                 +T DL  +  S+ DV++GVLDTG+WPESKS+ D G   IPS W+G CE+G +F+
Sbjct: 123 -----EHTADLFPEAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFT 182

Query: 185 PSLCNKKLIGARSFSKGYQMASGGGYFKKPREDQSARDQDGHGTHTASTAAGSHVANASL 244
            SLCN+KLIGAR F++GY+  S  G   + +E +S RD DGHGTHT+STAAGS V  ASL
Sbjct: 183 ASLCNRKLIGARFFARGYE--STMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASL 242

Query: 245 LGYARGIARGMAPQARVAAYKTCWPTGCFGSDILAAMDRAILDGVDVLSLSLGGGSAPYY 304
           LGYA G ARGMAP+ARVA YK CW  GCF SDILAA+D+AI D V+VLS+SLGGG + YY
Sbjct: 243 LGYASGTARGMAPRARVAVYKVCWLGGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYY 302

Query: 305 KDTIAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVDLGNG 364
           +D +AIGAFAAME+G+ VSCSAGN+GP+ +SL+NVAPWI TVGAGTLDRDFPA   LGNG
Sbjct: 303 RDGVAIGAFAAMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNG 362

Query: 365 RRITGVSLYSGQGMGSKPVGLVY--NKGSNSSSNVCLPGSLEPALVRGKVVICDRGVNAR 424
           +  TGVSL+ G+ +  K +  +Y  N  + ++ N+C+ G+L P  V+GK+V+CDRG+NAR
Sbjct: 363 KNFTGVSLFKGEALPDKLLPFIYAGNASNATNGNLCMTGTLIPEKVKGKIVMCDRGINAR 422

Query: 425 VEKGGVVRDAGGIGMILANTAASGEELVADSHLLPAVAVGRKVGDLIRQYVRSVSKPMAA 484
           V+KG VV+ AGG+GMILANTAA+GEELVAD+HLLPA  VG K GD+IR YV +   P A+
Sbjct: 423 VQKGDVVKAAGGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTAS 482

Query: 485 LSFGGTILNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESVGPTGLDSDK 544
           +S  GT++ V+PSPVVAAFSSRGPN +TP ILKPD+I PGVNILAAW+ + GPTGL SD 
Sbjct: 483 ISILGTVVGVKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDS 542

Query: 545 RKTQFNIMSGTSMSCPHISGLAALLKAAHSEWSPSAIKSALMTTAYTQDNTNSSLRDAAG 604
           R+ +FNI+SGTSMSCPH+SGLAALLK+ H EWSP+AI+SALMTTAY        L D A 
Sbjct: 543 RRVEFNIISGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIAT 602

Query: 605 GAFSNPWAHGAGHVDPHKALSPGLVYDISTDDYIDFLCSLDYGIDHVKAIVKRSNITCS- 664
           G  S P+ HGAGHV P  A +PGL+YD++T+DY+ FLC+L+Y    +++ V R N TC  
Sbjct: 603 GKPSTPFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRS-VSRRNYTCDP 662

Query: 665 RKFADPGQLNYPSFSVVFGSKRVVRYTRKVTNVGAAGSVYEVSTTAPSVVNVIVKPSKLV 724
            K      LNYPSF+V        +YTR VT+VG AG+     T+  + V + V+P+ L 
Sbjct: 663 SKSYSVADLNYPSFAVNVDGVGAYKYTRTVTSVGGAGTYSVKVTSETTGVKISVEPAVLN 722

Query: 725 FTKVGERKRYTITFVASRDAARTTKFGFGSIAWTNEQHQVKSPVAFAWT 755
           F +  E+K YT+TF         +   FGSI W++ +H V SPVA +WT
Sbjct: 723 FKEANEKKSYTVTFTVDSSKPSGSN-SFGSIEWSDGKHVVGSPVAISWT 757

BLAST of Sed0015954 vs. ExPASy Swiss-Prot
Match: Q9LVJ1 (Subtilisin-like protease SBT1.4 OS=Arabidopsis thaliana OX=3702 GN=SBT1.4 PE=2 SV=1)

HSP 1 Score: 743.8 bits (1919), Expect = 1.9e-213
Identity = 412/777 (53.02%), Postives = 511/777 (65.77%), Query Frame = 0

Query: 4   MAKFLMASLLLLLP---CVFINAKKT-----TYIVHMKHHALPSDYPTH-------LQSL 63
           MAK  ++S+  + P   C F  +  +     +YIVH++    PS + +H       L+SL
Sbjct: 1   MAKLSLSSIFFVFPLLLCFFSPSSSSSDGLESYIVHVQRSHKPSLFSSHNNWHVSLLRSL 60

Query: 64  -SSTASDSLLYTYTSAYHGFAASLDPDEAELLRQSDSVLGVYEDTVYNLHTTRTPGFLGL 123
            SS    +LLY+Y+ A HGF+A L P +   LR+  SV+ V  D    +HTT TP FLG 
Sbjct: 61  PSSPQPATLLYSYSRAVHGFSARLSPIQTAALRRHPSVISVIPDQAREIHTTHTPAFLGF 120

Query: 124 DSDFGLWEGHNTQDLDQASHDVIIGVLDTGIWPESKSFDDVGMPEIPSRWRGECESGPDF 183
             + GLW   N         DVI+GVLDTGIWPE  SF D G+  IPS W+GECE GPDF
Sbjct: 121 SQNSGLWSNSN------YGEDVIVGVLDTGIWPEHPSFSDSGLGPIPSTWKGECEIGPDF 180

Query: 184 SPSLCNKKLIGARSFSKGYQMASGGGYFKKPREDQSARDQDGHGTHTASTAAGSHVANAS 243
             S CN+KLIGAR+F +GY     G      +E +S RD +GHGTHTASTAAGS VANAS
Sbjct: 181 PASSCNRKLIGARAFYRGYLTQRNGTKKHAAKESRSPRDTEGHGTHTASTAAGSVVANAS 240

Query: 244 LLGYARGIARGMAPQARVAAYKTCWPTGCFGSDILAAMDRAILDGVDVLSLSLG-GGSAP 303
           L  YARG A GMA +AR+AAYK CW  GC+ SDILAAMD+A+ DGV V+SLS+G  GSAP
Sbjct: 241 LYQYARGTATGMASKARIAAYKICWTGGCYDSDILAAMDQAVADGVHVISLSVGASGSAP 300

Query: 304 -YYKDTIAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVDL 363
            Y+ D+IAIGAF A   G+ VSCSAGNSGPN  +  N+APWI+TVGA T+DR+F A    
Sbjct: 301 EYHTDSIAIGAFGATRHGIVVSCSAGNSGPNPETATNIAPWILTVGASTVDREFAANAIT 360

Query: 364 GNGRRITGVSLYSGQGMGSKPVGLVYNKGSNSSSNVCLPGSLEPALVRGKVVICDRGVNA 423
           G+G+  TG SLY+G+ +    + LVY+   +  S +C PG L  +LV GK+V+CDRG NA
Sbjct: 361 GDGKVFTGTSLYAGESLPDSQLSLVYS--GDCGSRLCYPGKLNSSLVEGKIVLCDRGGNA 420

Query: 424 RVEKGGVVRDAGGIGMILANTAASGEELVADSHLLPAVAVGRKVGDLIRQYVRSVSKPMA 483
           RVEKG  V+ AGG GMILANTA SGEEL ADSHL+PA  VG K GD IR Y+++   P A
Sbjct: 421 RVEKGSAVKLAGGAGMILANTAESGEELTADSHLVPATMVGAKAGDQIRDYIKTSDSPTA 480

Query: 484 ALSFGGTILNVR-PSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESVGPTGLDS 543
            +SF GT++    PSP VAAFSSRGPN +TP ILKPDVI PGVNILA W+  VGPT LD 
Sbjct: 481 KISFLGTLIGPSPPSPRVAAFSSRGPNHLTPVILKPDVIAPGVNILAGWTGMVGPTDLDI 540

Query: 544 DKRKTQFNIMSGTSMSCPHISGLAALLKAAHSEWSPSAIKSALMTTAYTQDNTNSSLRDA 603
           D R+ QFNI+SGTSMSCPH+SGLAALL+ AH +WSP+AIKSAL+TTAY  +N+   + D 
Sbjct: 541 DPRRVQFNIISGTSMSCPHVSGLAALLRKAHPDWSPAAIKSALVTTAYDVENSGEPIEDL 600

Query: 604 AGGAFSNPWAHGAGHVDPHKALSPGLVYDISTDDYIDFLCSLDYGIDHVKAIVKRSNI-- 663
           A G  SN + HGAGHVDP+KAL+PGLVYDI   +Y+ FLC++ Y    +   ++   +  
Sbjct: 601 ATGKSSNSFIHGAGHVDPNKALNPGLVYDIEVKEYVAFLCAVGYEFPGILVFLQDPTLYD 660

Query: 664 TC-SRKFADPGQLNYPSFSVVFGSK-RVVRYTRKVTNVGA-AGSVYEVSTTAPSVVNVIV 723
            C + K    G LNYPSFSVVF S   VV+Y R V NVG+   +VYEV   +P+ V + V
Sbjct: 661 ACETSKLRTAGDLNYPSFSVVFASTGEVVKYKRVVKNVGSNVDAVYEVGVKSPANVEIDV 720

Query: 724 KPSKLVFTKVGERKRYTITF---VASRDAARTTKFGFGSIAWTNEQHQVKSPVAFAW 754
            PSKL F+K      Y +TF   V            FGSI WT+ +H VKSPVA  W
Sbjct: 721 SPSKLAFSKEKSVLEYEVTFKSVVLGGGVGSVPGHEFGSIEWTDGEHVVKSPVAVQW 769

BLAST of Sed0015954 vs. ExPASy Swiss-Prot
Match: Q9FLI4 (Subtilisin-like protease SBT1.3 OS=Arabidopsis thaliana OX=3702 GN=SBT1.3 PE=2 SV=1)

HSP 1 Score: 732.6 bits (1890), Expect = 4.4e-210
Identity = 391/772 (50.65%), Postives = 507/772 (65.67%), Query Frame = 0

Query: 12  LLLLLPCVFINAK-------KTTYIVHMKHHALPSDYPTHLQSLSSTA------------ 71
           ++L +  +F+ A+       K TY++HM   A+P  Y  HLQ  SS              
Sbjct: 14  IILSINLIFLQAETTTQISTKKTYVIHMDKSAMPLPYTNHLQWYSSKINSVTQHKSQEEE 73

Query: 72  --SDSLLYTYTSAYHGFAASLDPDEAELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSD 131
             ++ +LYTY +A+HG AA L  +EAE L + D V+ V  +T Y LHTTR+P FLGL+  
Sbjct: 74  GNNNRILYTYQTAFHGLAAQLTQEEAERLEEEDGVVAVIPETRYELHTTRSPTFLGLERQ 133

Query: 132 FGLWEGHNTQDLDQASHDVIIGVLDTGIWPESKSFDDVGMPEIPSRWRGECESGPDFSPS 191
               E           HDV++GVLDTGIWPES+SF+D GM  +P+ WRG CE+G  F   
Sbjct: 134 ----ESERVWAERVTDHDVVVGVLDTGIWPESESFNDTGMSPVPATWRGACETGKRFLKR 193

Query: 192 LCNKKLIGARSFSKGYQMASGGGYFKKPREDQSARDQDGHGTHTASTAAGSHVANASLLG 251
            CN+K++GAR F +GY+ A+  G   +  E +S RD+DGHGTHTA+T AGS V  A+L G
Sbjct: 194 NCNRKIVGARVFYRGYEAAT--GKIDEELEYKSPRDRDGHGTHTAATVAGSPVKGANLFG 253

Query: 252 YARGIARGMAPQARVAAYKTCWPTGCFGSDILAAMDRAILDGVDVLSLSLGGGSAPYYKD 311
           +A G ARGMA +ARVAAYK CW  GCF SDIL+A+D+A+ DGV VLS+SLGGG + Y +D
Sbjct: 254 FAYGTARGMAQKARVAAYKVCWVGGCFSSDILSAVDQAVADGVQVLSISLGGGVSTYSRD 313

Query: 312 TIAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVDLGNGRR 371
           +++I  F AME GVFVSCSAGN GP+  SL NV+PWI TVGA T+DRDFPA V +G  R 
Sbjct: 314 SLSIATFGAMEMGVFVSCSAGNGGPDPISLTNVSPWITTVGASTMDRDFPATVKIGTMRT 373

Query: 372 ITGVSLYSGQGM--GSKPVGLVY---NKGSNSSSNVCLPGSLEPALVRGKVVICDRGVNA 431
             GVSLY G+ +   +K   LVY   N  S   ++ CL G+L+   V GK+VICDRGV  
Sbjct: 374 FKGVSLYKGRTVLPKNKQYPLVYLGRNASSPDPTSFCLDGALDRRHVAGKIVICDRGVTP 433

Query: 432 RVEKGGVVRDAGGIGMILANTAASGEELVADSHLLPAVAVGRKVGDLIRQYVRSVSKPMA 491
           RV+KG VV+ AGGIGM+L NTA +GEELVADSH+LPAVAVG K G LI+QY  +  K  A
Sbjct: 434 RVQKGQVVKRAGGIGMVLTNTATNGEELVADSHMLPAVAVGEKEGKLIKQYAMTSKKATA 493

Query: 492 ALSFGGTILNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESVGPTGLDSD 551
           +L   GT + ++PSPVVAAFSSRGPN ++ +ILKPD++ PGVNILAAW+  + P+ L SD
Sbjct: 494 SLEILGTRIGIKPSPVVAAFSSRGPNFLSLEILKPDLLAPGVNILAAWTGDMAPSSLSSD 553

Query: 552 KRKTQFNIMSGTSMSCPHISGLAALLKAAHSEWSPSAIKSALMTTAYTQDNTNSSLRDAA 611
            R+ +FNI+SGTSMSCPH+SG+AAL+K+ H +WSP+AIKSALMTTAY  DN    L DA+
Sbjct: 554 PRRVKFNILSGTSMSCPHVSGVAALIKSRHPDWSPAAIKSALMTTAYVHDNMFKPLTDAS 613

Query: 612 GGAFSNPWAHGAGHVDPHKALSPGLVYDISTDDYIDFLCSLDYGIDHVKAIVKRSNITCS 671
           G A S+P+ HGAGH+DP +A  PGLVYDI   +Y +FLC+ D     +K   K SN TC 
Sbjct: 614 GAAPSSPYDHGAGHIDPLRATDPGLVYDIGPQEYFEFLCTQDLSPSQLKVFTKHSNRTCK 673

Query: 672 RKFA-DPGQLNYPSFSVVFGSK---RVVRYTRKVTNVGAAGSVYEVSTTAPSVVNVIVKP 731
              A +PG LNYP+ S +F      + +   R VTNVG   S Y+VS +     +V V+P
Sbjct: 674 HTLAKNPGNLNYPAISALFPENTHVKAMTLRRTVTNVGPHISSYKVSVSPFKGASVTVQP 733

Query: 732 SKLVFTKVGERKRYTITFVASRDAARTTKFGFGSIAWTNEQHQVKSPVAFAW 754
             L FT   ++  YT+TF   R   R  +  FG + W +  H+V+SPV   W
Sbjct: 734 KTLNFTSKHQKLSYTVTF---RTRFRMKRPEFGGLVWKSTTHKVRSPVIITW 776

BLAST of Sed0015954 vs. ExPASy Swiss-Prot
Match: O49607 (Subtilisin-like protease SBT1.6 OS=Arabidopsis thaliana OX=3702 GN=SBT1.6 PE=2 SV=1)

HSP 1 Score: 718.8 bits (1854), Expect = 6.6e-206
Identity = 386/757 (50.99%), Postives = 519/757 (68.56%), Query Frame = 0

Query: 12  LLLLLPCVFINAKKT----TYIVHMKHHALPSDYPTHLQSLSSTASDS--LLYTYTSAYH 71
           LLL L   FI+   +    T+I  +   ++PS +PTH    S+  ++   +++ Y + +H
Sbjct: 8   LLLFLSFPFISFAASQAAKTFIFRIDGGSMPSIFPTHYHWYSTEFAEESRIVHVYHTVFH 67

Query: 72  GFAASLDPDEAELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSDFGLWEGHNTQDLDQA 131
           GF+A + PDEA+ LR   +VL V+ED    LHTTR+P FLGL +  GLW      + D  
Sbjct: 68  GFSAVVTPDEADNLRNHPAVLAVFEDRRRELHTTRSPQFLGLQNQKGLW-----SESDYG 127

Query: 132 SHDVIIGVLDTGIWPESKSFDDVGMPEIPSRWRGECESGPDFSPSLCNKKLIGARSFSKG 191
           S DVIIGV DTGIWPE +SF D+ +  IP RWRG CESG  FSP  CN+K+IGAR F+KG
Sbjct: 128 S-DVIIGVFDTGIWPERRSFSDLNLGPIPKRWRGVCESGARFSPRNCNRKIIGARFFAKG 187

Query: 192 YQMASGGGYFKKPREDQSARDQDGHGTHTASTAAGSHVANASLLGYARGIARGMAPQARV 251
            Q A  GG   K  E  S RD DGHGTHT+STAAG H   AS+ GYA G+A+G+AP+AR+
Sbjct: 188 QQAAVIGG-INKTVEFLSPRDADGHGTHTSSTAAGRHAFKASMSGYASGVAKGVAPKARI 247

Query: 252 AAYKTCW-PTGCFGSDILAAMDRAILDGVDVLSLSLGGG---SAPYYKDTIAIGAFAAME 311
           AAYK CW  +GC  SDILAA D A+ DGVDV+S+S+GGG   ++PYY D IAIG++ A  
Sbjct: 248 AAYKVCWKDSGCLDSDILAAFDAAVRDGVDVISISIGGGDGITSPYYLDPIAIGSYGAAS 307

Query: 312 KGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVDLGNGRRITGVSLYSGQG 371
           KG+FVS SAGN GPN  S+ N+APW+ TVGA T+DR+FPA   LG+G R+ GVSLY+G  
Sbjct: 308 KGIFVSSSAGNEGPNGMSVTNLAPWVTTVGASTIDRNFPADAILGDGHRLRGVSLYAGVP 367

Query: 372 MGSKPVGLVY-NKGSNSSSNVCLPGSLEPALVRGKVVICDRGVNARVEKGGVVRDAGGIG 431
           +  +   +VY  K   SS+++C+  +L+P  VRGK+VICDRG + RV KG VV+ AGG+G
Sbjct: 368 LNGRMFPVVYPGKSGMSSASLCMENTLDPKQVRGKIVICDRGSSPRVAKGLVVKKAGGVG 427

Query: 432 MILANTAASGEELVADSHLLPAVAVGRKVGDLIRQYVRSVSKPMAALSFGGTILNVRPSP 491
           MILAN A++GE LV D+HL+PA AVG   GD I+ Y  S   P+A++ F GTI+ ++P+P
Sbjct: 428 MILANGASNGEGLVGDAHLIPACAVGSNEGDRIKAYASSHPNPIASIDFRGTIVGIKPAP 487

Query: 492 VVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESVGPTGLDSDKRKTQFNIMSGTSMS 551
           V+A+FS RGPN ++P+ILKPD+I PGVNILAAW+++VGPTGL SD RKT+FNI+SGTSM+
Sbjct: 488 VIASFSGRGPNGLSPEILKPDLIAPGVNILAAWTDAVGPTGLPSDPRKTEFNILSGTSMA 547

Query: 552 CPHISGLAALLKAAHSEWSPSAIKSALMTTAYTQDNTNSSLRDAAGGAFSNPWAHGAGHV 611
           CPH+SG AALLK+AH +WSP+ I+SA+MTT    DN+N SL D + G  + P+ +G+GH+
Sbjct: 548 CPHVSGAAALLKSAHPDWSPAVIRSAMMTTTNLVDNSNRSLIDESTGKSATPYDYGSGHL 607

Query: 612 DPHKALSPGLVYDISTDDYIDFLCSLDYGIDHVKAIVKRSNITC-SRKFADPGQLNYPSF 671
           +  +A++PGLVYDI+ DDYI FLCS+ YG   ++ ++ R+ + C + +   PG LNYPS 
Sbjct: 608 NLGRAMNPGLVYDITNDDYITFLCSIGYGPKTIQ-VITRTPVRCPTTRKPSPGNLNYPSI 667

Query: 672 SVVFGSKR--VVRYT--RKVTNVGAAGSVYEVSTTAPSVVNVIVKPSKLVFTKVGERKRY 731
           + VF + R  +V  T  R  TNVG A +VY     +P  V V VKP +LVFT   +R+ Y
Sbjct: 668 TAVFPTNRRGLVSKTVIRTATNVGQAEAVYRARIESPRGVTVTVKPPRLVFTSAVKRRSY 727

Query: 732 TITFVASRDAARTTKFG--FGSIAW-TNEQHQVKSPV 750
            +T   +       + G  FGS+ W    +H V+SP+
Sbjct: 728 AVTVTVNTRNVVLGETGAVFGSVTWFDGGKHVVRSPI 756

BLAST of Sed0015954 vs. ExPASy TrEMBL
Match: A0A6J1H8F1 (subtilisin-like protease SBT1.8 OS=Cucurbita moschata OX=3662 GN=LOC111461488 PE=3 SV=1)

HSP 1 Score: 1391.7 bits (3601), Expect = 0.0e+00
Identity = 702/767 (91.53%), Postives = 731/767 (95.31%), Query Frame = 0

Query: 1   MGSMAKFLMAS---LLLLLPCVFINAKKTTYIVHMKHHALPSDYPT-------HLQSLSS 60
           MGSMA+ L+AS   LLLLLPCVF+NAK+ TYIV MKHHALPSDY T       HLQSLSS
Sbjct: 1   MGSMARLLVASFLLLLLLLPCVFVNAKR-TYIVRMKHHALPSDYLTHHDWYSAHLQSLSS 60

Query: 61  TA-SDSLLYTYTSAYHGFAASLDPDEAELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDS 120
           +A SDSLLYTYTSAYHGFAASLDPDEAELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDS
Sbjct: 61  SATSDSLLYTYTSAYHGFAASLDPDEAELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDS 120

Query: 121 DFGLWEGHNTQDLDQASHDVIIGVLDTGIWPESKSFDDVGMPEIPSRWRGECESGPDFSP 180
           DFGLWEGHNTQDLDQASHDVIIGVLDTGIWPESKSFDD GMPEIPSRWRGECESGPDFSP
Sbjct: 121 DFGLWEGHNTQDLDQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGECESGPDFSP 180

Query: 181 SLCNKKLIGARSFSKGYQMASGGGYFKKPREDQSARDQDGHGTHTASTAAGSHVANASLL 240
           SLCNKKLIGARSFSKGYQMASGGGYF+K RE++S RDQDGHGTHTASTAAGSHVANASLL
Sbjct: 181 SLCNKKLIGARSFSKGYQMASGGGYFRKSRENESPRDQDGHGTHTASTAAGSHVANASLL 240

Query: 241 GYARGIARGMAPQARVAAYKTCWPTGCFGSDILAAMDRAILDGVDVLSLSLGGGSAPYYK 300
           GYARGIARGMAPQARVAAYKTCWPTGCFGSDILA MDRAI DGVDVLSLSLGGGSAPYY+
Sbjct: 241 GYARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYR 300

Query: 301 DTIAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVDLGNGR 360
           DTIAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYV LGNG+
Sbjct: 301 DTIAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGK 360

Query: 361 RITGVSLYSGQGMGSKPVGLVYNKGSNSSSNVCLPGSLEPALVRGKVVICDRGVNARVEK 420
           R TGVSLYSGQGMG+KPV LVYNKGSNSSSN+CLPGSLEPA VRGKVVICDRG+NARVEK
Sbjct: 361 RFTGVSLYSGQGMGNKPVALVYNKGSNSSSNMCLPGSLEPAFVRGKVVICDRGINARVEK 420

Query: 421 GGVVRDAGGIGMILANTAASGEELVADSHLLPAVAVGRKVGDLIRQYVRSVSKPMAALSF 480
           GGVVRDAGGIGMILANTAASGEELVADSHLLPAVAVG K GDLIRQYVRSV+KP A LSF
Sbjct: 421 GGVVRDAGGIGMILANTAASGEELVADSHLLPAVAVGAKTGDLIRQYVRSVAKPTAVLSF 480

Query: 481 GGTILNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESVGPTGLDSDKRKT 540
           GGT+LNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSES+GPTGLD+DKRKT
Sbjct: 481 GGTVLNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDNDKRKT 540

Query: 541 QFNIMSGTSMSCPHISGLAALLKAAHSEWSPSAIKSALMTTAYTQDNTNSSLRDAAGGAF 600
           QFNIMSGTSMSCPHISGLAALLKAAH  WSPSAIKSALMTTAYTQDNTNSSLRDAAGGAF
Sbjct: 541 QFNIMSGTSMSCPHISGLAALLKAAHPSWSPSAIKSALMTTAYTQDNTNSSLRDAAGGAF 600

Query: 601 SNPWAHGAGHVDPHKALSPGLVYDISTDDYIDFLCSLDYGIDHVKAIVKRSNITCSRKFA 660
           SNPWAHGAGHVDPHKALSPGLVYDIST+DYI FLCSLDYGIDHV+AI KRSNITC +KFA
Sbjct: 601 SNPWAHGAGHVDPHKALSPGLVYDISTNDYITFLCSLDYGIDHVQAIAKRSNITCLKKFA 660

Query: 661 DPGQLNYPSFSVVFGSKRVVRYTRKVTNVGAAGSVYEVSTTAPSVVNVIVKPSKLVFTKV 720
           DPGQLNYPSFSVVFGSKRVVRYTR VTNVGAAGSVYEV+TTAPSVV V VKPSKLVF+KV
Sbjct: 661 DPGQLNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVYEVATTAPSVVKVTVKPSKLVFSKV 720

Query: 721 GERKRYTITFVASRDAARTTKFGFGSIAWTNEQHQVKSPVAFAWTRL 757
           GERKRYT+TFVASRDAARTT++GFGSIAW+N+QHQV+SPVAFAWT+L
Sbjct: 721 GERKRYTVTFVASRDAARTTRYGFGSIAWSNDQHQVRSPVAFAWTKL 766

BLAST of Sed0015954 vs. ExPASy TrEMBL
Match: A0A6J1JIF3 (subtilisin-like protease SBT1.8 OS=Cucurbita maxima OX=3661 GN=LOC111484737 PE=3 SV=1)

HSP 1 Score: 1390.2 bits (3597), Expect = 0.0e+00
Identity = 701/767 (91.40%), Postives = 731/767 (95.31%), Query Frame = 0

Query: 1   MGSMAKFLMAS---LLLLLPCVFINAKKTTYIVHMKHHALPSDYPT-------HLQSLSS 60
           MGSMA+ L+AS   LLLLLPCVF+NAK+ TYIV MKHHALPSDY T       HLQSLSS
Sbjct: 1   MGSMARLLVASFLLLLLLLPCVFVNAKR-TYIVRMKHHALPSDYLTHHDWYSAHLQSLSS 60

Query: 61  TA-SDSLLYTYTSAYHGFAASLDPDEAELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDS 120
           +A SDSLLYTYTSAYHGFAASLDPDEAELLRQSDSVLGVYEDTVY LHTTRTPGFLGLDS
Sbjct: 61  SATSDSLLYTYTSAYHGFAASLDPDEAELLRQSDSVLGVYEDTVYKLHTTRTPGFLGLDS 120

Query: 121 DFGLWEGHNTQDLDQASHDVIIGVLDTGIWPESKSFDDVGMPEIPSRWRGECESGPDFSP 180
           DFGLWEGHNTQDLDQASHDVIIGVLDTGIWPESKSFDD GMPEIPSRWRGECESGPDFSP
Sbjct: 121 DFGLWEGHNTQDLDQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGECESGPDFSP 180

Query: 181 SLCNKKLIGARSFSKGYQMASGGGYFKKPREDQSARDQDGHGTHTASTAAGSHVANASLL 240
           SLCNKKLIGARSFSKGYQMASGGGYF+K RE++S RDQDGHGTHTASTAAGSHVANASLL
Sbjct: 181 SLCNKKLIGARSFSKGYQMASGGGYFRKSRENESPRDQDGHGTHTASTAAGSHVANASLL 240

Query: 241 GYARGIARGMAPQARVAAYKTCWPTGCFGSDILAAMDRAILDGVDVLSLSLGGGSAPYYK 300
           GYARGIARGMAPQARVAAYKTCWPTGCFGSDILA MDRAI DGVDVLSLSLGGGSAPYY+
Sbjct: 241 GYARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYR 300

Query: 301 DTIAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVDLGNGR 360
           DTIAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYV LGNG+
Sbjct: 301 DTIAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGK 360

Query: 361 RITGVSLYSGQGMGSKPVGLVYNKGSNSSSNVCLPGSLEPALVRGKVVICDRGVNARVEK 420
           R TGVSLYSGQGMG+KPV LVYNKGSNSSSN+CLPGSLEPA VRGKVVICDRG+NARVEK
Sbjct: 361 RFTGVSLYSGQGMGNKPVALVYNKGSNSSSNMCLPGSLEPAFVRGKVVICDRGINARVEK 420

Query: 421 GGVVRDAGGIGMILANTAASGEELVADSHLLPAVAVGRKVGDLIRQYVRSVSKPMAALSF 480
           GGVVRDAGGIGMILANTAASGEELVADSHLLPAVAVG K GDLIRQYVRSV+KP A LSF
Sbjct: 421 GGVVRDAGGIGMILANTAASGEELVADSHLLPAVAVGAKTGDLIRQYVRSVAKPTAVLSF 480

Query: 481 GGTILNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESVGPTGLDSDKRKT 540
           GGT+LNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSES+GPTGLD+DKRKT
Sbjct: 481 GGTVLNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDNDKRKT 540

Query: 541 QFNIMSGTSMSCPHISGLAALLKAAHSEWSPSAIKSALMTTAYTQDNTNSSLRDAAGGAF 600
           QFNIMSGTSMSCPHISGLAALLKAAH  WSPSAIKSALMTTAYTQDNTNSSLRDAAGG F
Sbjct: 541 QFNIMSGTSMSCPHISGLAALLKAAHPSWSPSAIKSALMTTAYTQDNTNSSLRDAAGGMF 600

Query: 601 SNPWAHGAGHVDPHKALSPGLVYDISTDDYIDFLCSLDYGIDHVKAIVKRSNITCSRKFA 660
           SNPWAHGAGHVDPHKALSPGL+YDIST+DYI FLCSLDYGIDHV+AIVKRSNITCS+KFA
Sbjct: 601 SNPWAHGAGHVDPHKALSPGLLYDISTNDYITFLCSLDYGIDHVQAIVKRSNITCSKKFA 660

Query: 661 DPGQLNYPSFSVVFGSKRVVRYTRKVTNVGAAGSVYEVSTTAPSVVNVIVKPSKLVFTKV 720
           DPGQLNYPSFSVVFGSKRVVRYTR VTNVGAAGSVYEV+TTAPSVV V VKPSKLVF+KV
Sbjct: 661 DPGQLNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVYEVATTAPSVVKVTVKPSKLVFSKV 720

Query: 721 GERKRYTITFVASRDAARTTKFGFGSIAWTNEQHQVKSPVAFAWTRL 757
           GERKRYT+TFVASRDAARTT++GFGSIAW+N+QHQV+SPVAFAWT+L
Sbjct: 721 GERKRYTVTFVASRDAARTTRYGFGSIAWSNDQHQVRSPVAFAWTKL 766

BLAST of Sed0015954 vs. ExPASy TrEMBL
Match: A0A5A7UKV3 (Subtilisin-like protease SBT1.8 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold181G001560 PE=3 SV=1)

HSP 1 Score: 1362.8 bits (3526), Expect = 0.0e+00
Identity = 687/766 (89.69%), Postives = 724/766 (94.52%), Query Frame = 0

Query: 1   MGSMAKFLM--ASLLLLLPCVFINAKKTTYIVHMKHHALPSDYPTH-------LQSL-SS 60
           M SM +FL+    LLLLL CVFINAKK TYIVHMKHHALPS+Y TH       LQSL SS
Sbjct: 1   MDSMPRFLIPFLLLLLLLSCVFINAKK-TYIVHMKHHALPSEYLTHHDWYSASLQSLSSS 60

Query: 61  TASDSLLYTYTSAYHGFAASLDPDEAELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSD 120
           ++SDSLLYTYTS++HGFAA LD +E ELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSD
Sbjct: 61  SSSDSLLYTYTSSFHGFAAFLDSEEVELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSD 120

Query: 121 FGLWEGHNTQDLDQASHDVIIGVLDTGIWPESKSFDDVGMPEIPSRWRGECESGPDFSPS 180
           FGLWEGH TQDL+QASHDVIIGVLDTGIWPESKSFDD GMPEIPSRWRG CE+GPDFSPS
Sbjct: 121 FGLWEGHTTQDLNQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGGCEAGPDFSPS 180

Query: 181 LCNKKLIGARSFSKGYQMASGGGYFKKPREDQSARDQDGHGTHTASTAAGSHVANASLLG 240
           LCNKKLIGARSFSKGYQMASGGGYF+KPRE+QSARDQDGHGTHTASTAAGSHV NASLLG
Sbjct: 181 LCNKKLIGARSFSKGYQMASGGGYFRKPRENQSARDQDGHGTHTASTAAGSHVPNASLLG 240

Query: 241 YARGIARGMAPQARVAAYKTCWPTGCFGSDILAAMDRAILDGVDVLSLSLGGGSAPYYKD 300
           YARGIARGMAPQARVAAYKTCWPTGCFGSDILA MDRAI DGVDVLSLSLGGGSAPYY+D
Sbjct: 241 YARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRD 300

Query: 301 TIAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVDLGNGRR 360
           TIAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYV LGNG+R
Sbjct: 301 TIAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKR 360

Query: 361 ITGVSLYSGQGMGSKPVGLVYNKGSNSSSNVCLPGSLEPALVRGKVVICDRGVNARVEKG 420
            TGVSLYSGQGMG+K V LVYNKGSN+SSN+CLPGSL+PA+VRGKVV+CDRG+NARVEKG
Sbjct: 361 FTGVSLYSGQGMGNKAVALVYNKGSNTSSNMCLPGSLDPAVVRGKVVVCDRGINARVEKG 420

Query: 421 GVVRDAGGIGMILANTAASGEELVADSHLLPAVAVGRKVGDLIRQYVRSVSKPMAALSFG 480
           GVVRDAGGIGMILANTAASGEELVADSHLLPAVAVGRK GDLIRQYVRS S P A LSFG
Sbjct: 421 GVVRDAGGIGMILANTAASGEELVADSHLLPAVAVGRKTGDLIRQYVRSDSNPTAVLSFG 480

Query: 481 GTILNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESVGPTGLDSDKRKTQ 540
           GTILNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSES+GPTGL++DKRKTQ
Sbjct: 481 GTILNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLENDKRKTQ 540

Query: 541 FNIMSGTSMSCPHISGLAALLKAAHSEWSPSAIKSALMTTAYTQDNTNSSLRDAAGGAFS 600
           FNIMSGTSMSCPHISGLAALLKAAH +WSPSAIKSALMTTAYTQDNTNSSLRDAAGG FS
Sbjct: 541 FNIMSGTSMSCPHISGLAALLKAAHPQWSPSAIKSALMTTAYTQDNTNSSLRDAAGGGFS 600

Query: 601 NPWAHGAGHVDPHKALSPGLVYDISTDDYIDFLCSLDYGIDHVKAIVKRSNITCSRKFAD 660
           NPWAHGAGHVDPHKALSPGL+YDIST+DYI FLCSLDYGIDHV+AIVKRSNITCSRKFAD
Sbjct: 601 NPWAHGAGHVDPHKALSPGLLYDISTNDYIAFLCSLDYGIDHVQAIVKRSNITCSRKFAD 660

Query: 661 PGQLNYPSFSVVFGSKRVVRYTRKVTNVGAAGSVYEVSTTAPSVVNVIVKPSKLVFTKVG 720
           PGQLNYPSFSVVFGSKRVVRYTR VTNVGAAGSVY+V+TTAPSVV V VKPSKLVFTKVG
Sbjct: 661 PGQLNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVYDVATTAPSVVKVTVKPSKLVFTKVG 720

Query: 721 ERKRYTITFVASRDAARTTKFGFGSIAWTNEQHQVKSPVAFAWTRL 757
           ERKRYT+TFVASRDAA+TT+FGFGSI W+N+QHQV+SPV+FAWTRL
Sbjct: 721 ERKRYTVTFVASRDAAQTTRFGFGSIVWSNDQHQVRSPVSFAWTRL 765

BLAST of Sed0015954 vs. ExPASy TrEMBL
Match: A0A1S3BQE8 (subtilisin-like protease SBT1.8 OS=Cucumis melo OX=3656 GN=LOC103492374 PE=3 SV=1)

HSP 1 Score: 1362.8 bits (3526), Expect = 0.0e+00
Identity = 687/766 (89.69%), Postives = 724/766 (94.52%), Query Frame = 0

Query: 1   MGSMAKFLM--ASLLLLLPCVFINAKKTTYIVHMKHHALPSDYPTH-------LQSL-SS 60
           M SM +FL+    LLLLL CVFINAKK TYIVHMKHHALPS+Y TH       LQSL SS
Sbjct: 1   MDSMPRFLIPFLLLLLLLSCVFINAKK-TYIVHMKHHALPSEYLTHHDWYSASLQSLSSS 60

Query: 61  TASDSLLYTYTSAYHGFAASLDPDEAELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSD 120
           ++SDSLLYTYTS++HGFAA LD +E ELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSD
Sbjct: 61  SSSDSLLYTYTSSFHGFAAFLDSEEVELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSD 120

Query: 121 FGLWEGHNTQDLDQASHDVIIGVLDTGIWPESKSFDDVGMPEIPSRWRGECESGPDFSPS 180
           FGLWEGH TQDL+QASHDVIIGVLDTGIWPESKSFDD GMPEIPSRWRG CE+GPDFSPS
Sbjct: 121 FGLWEGHTTQDLNQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGGCEAGPDFSPS 180

Query: 181 LCNKKLIGARSFSKGYQMASGGGYFKKPREDQSARDQDGHGTHTASTAAGSHVANASLLG 240
           LCNKKLIGARSFSKGYQMASGGGYF+KPRE+QSARDQDGHGTHTASTAAGSHV NASLLG
Sbjct: 181 LCNKKLIGARSFSKGYQMASGGGYFRKPRENQSARDQDGHGTHTASTAAGSHVPNASLLG 240

Query: 241 YARGIARGMAPQARVAAYKTCWPTGCFGSDILAAMDRAILDGVDVLSLSLGGGSAPYYKD 300
           YARGIARGMAPQARVAAYKTCWPTGCFGSDILA MDRAI DGVDVLSLSLGGGSAPYY+D
Sbjct: 241 YARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRD 300

Query: 301 TIAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVDLGNGRR 360
           TIAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYV LGNG+R
Sbjct: 301 TIAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKR 360

Query: 361 ITGVSLYSGQGMGSKPVGLVYNKGSNSSSNVCLPGSLEPALVRGKVVICDRGVNARVEKG 420
            TGVSLYSGQGMG+K V LVYNKGSN+SSN+CLPGSL+PA+VRGKVV+CDRG+NARVEKG
Sbjct: 361 FTGVSLYSGQGMGNKAVALVYNKGSNTSSNMCLPGSLDPAVVRGKVVVCDRGINARVEKG 420

Query: 421 GVVRDAGGIGMILANTAASGEELVADSHLLPAVAVGRKVGDLIRQYVRSVSKPMAALSFG 480
           GVVRDAGGIGMILANTAASGEELVADSHLLPAVAVGRK GDLIRQYVRS S P A LSFG
Sbjct: 421 GVVRDAGGIGMILANTAASGEELVADSHLLPAVAVGRKTGDLIRQYVRSDSNPTAVLSFG 480

Query: 481 GTILNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESVGPTGLDSDKRKTQ 540
           GTILNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSES+GPTGL++DKRKTQ
Sbjct: 481 GTILNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLENDKRKTQ 540

Query: 541 FNIMSGTSMSCPHISGLAALLKAAHSEWSPSAIKSALMTTAYTQDNTNSSLRDAAGGAFS 600
           FNIMSGTSMSCPHISGLAALLKAAH +WSPSAIKSALMTTAYTQDNTNSSLRDAAGG FS
Sbjct: 541 FNIMSGTSMSCPHISGLAALLKAAHPQWSPSAIKSALMTTAYTQDNTNSSLRDAAGGGFS 600

Query: 601 NPWAHGAGHVDPHKALSPGLVYDISTDDYIDFLCSLDYGIDHVKAIVKRSNITCSRKFAD 660
           NPWAHGAGHVDPHKALSPGL+YDIST+DYI FLCSLDYGIDHV+AIVKRSNITCSRKFAD
Sbjct: 601 NPWAHGAGHVDPHKALSPGLLYDISTNDYIAFLCSLDYGIDHVQAIVKRSNITCSRKFAD 660

Query: 661 PGQLNYPSFSVVFGSKRVVRYTRKVTNVGAAGSVYEVSTTAPSVVNVIVKPSKLVFTKVG 720
           PGQLNYPSFSVVFGSKRVVRYTR VTNVGAAGSVY+V+TTAPSVV V VKPSKLVFTKVG
Sbjct: 661 PGQLNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVYDVATTAPSVVKVTVKPSKLVFTKVG 720

Query: 721 ERKRYTITFVASRDAARTTKFGFGSIAWTNEQHQVKSPVAFAWTRL 757
           ERKRYT+TFVASRDAA+TT+FGFGSI W+N+QHQV+SPV+FAWTRL
Sbjct: 721 ERKRYTVTFVASRDAAQTTRFGFGSIVWSNDQHQVRSPVSFAWTRL 765

BLAST of Sed0015954 vs. ExPASy TrEMBL
Match: A0A5D3CDV9 (Subtilisin-like protease SBT1.8 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G001530 PE=3 SV=1)

HSP 1 Score: 1362.1 bits (3524), Expect = 0.0e+00
Identity = 686/763 (89.91%), Postives = 723/763 (94.76%), Query Frame = 0

Query: 1   MGSMAKFLMA-SLLLLLPCVFINAKKTTYIVHMKHHALPSDYPTH-------LQSL-SST 60
           M SM +FL+   LLLLL CVFINAKK TYIVHMKHHALPS+Y TH       LQSL SS+
Sbjct: 1   MDSMPRFLIPFLLLLLLSCVFINAKK-TYIVHMKHHALPSEYLTHHDWYSASLQSLSSSS 60

Query: 61  ASDSLLYTYTSAYHGFAASLDPDEAELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSDF 120
           +SDSLLYTYTS++HGFAA LD +E ELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSDF
Sbjct: 61  SSDSLLYTYTSSFHGFAAFLDSEEVELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSDF 120

Query: 121 GLWEGHNTQDLDQASHDVIIGVLDTGIWPESKSFDDVGMPEIPSRWRGECESGPDFSPSL 180
           GLWEGH TQDL+QASHDVIIGVLDTGIWPESKSFDD GMPEIPSRWRGECE+GPDFSPSL
Sbjct: 121 GLWEGHTTQDLNQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGECEAGPDFSPSL 180

Query: 181 CNKKLIGARSFSKGYQMASGGGYFKKPREDQSARDQDGHGTHTASTAAGSHVANASLLGY 240
           CNKKLIGARSFSKGYQMASGGGYF+KPRE+QSARDQDGHGTHTASTAAGSHV NASLLGY
Sbjct: 181 CNKKLIGARSFSKGYQMASGGGYFRKPRENQSARDQDGHGTHTASTAAGSHVPNASLLGY 240

Query: 241 ARGIARGMAPQARVAAYKTCWPTGCFGSDILAAMDRAILDGVDVLSLSLGGGSAPYYKDT 300
           ARGIARGMAPQARVAAYKTCWPTGCFGSDILA MDRAI DGVDVLSLSLGGGSAPYY+DT
Sbjct: 241 ARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRDT 300

Query: 301 IAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVDLGNGRRI 360
           IAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYV LGNG+R 
Sbjct: 301 IAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRF 360

Query: 361 TGVSLYSGQGMGSKPVGLVYNKGSNSSSNVCLPGSLEPALVRGKVVICDRGVNARVEKGG 420
           TGVSLYSGQGMG+K V LVYNKGSN+SSN+CLPGSL+PA+VRGKVV+CDRG+NARVEKGG
Sbjct: 361 TGVSLYSGQGMGNKAVALVYNKGSNTSSNMCLPGSLDPAVVRGKVVVCDRGINARVEKGG 420

Query: 421 VVRDAGGIGMILANTAASGEELVADSHLLPAVAVGRKVGDLIRQYVRSVSKPMAALSFGG 480
           VVRDAGGIGMILANTAASGEELVADSHLLPAVAVGRK GDLIRQYVRS S P A LSFGG
Sbjct: 421 VVRDAGGIGMILANTAASGEELVADSHLLPAVAVGRKTGDLIRQYVRSDSNPTAVLSFGG 480

Query: 481 TILNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESVGPTGLDSDKRKTQF 540
           TILNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSES+GPTGL++DKRKTQF
Sbjct: 481 TILNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLENDKRKTQF 540

Query: 541 NIMSGTSMSCPHISGLAALLKAAHSEWSPSAIKSALMTTAYTQDNTNSSLRDAAGGAFSN 600
           NIMSGTSMSCPHISGLAALLKAAH +WSPSAIKSALMTTAYTQDNTNSSLRDAAGG FSN
Sbjct: 541 NIMSGTSMSCPHISGLAALLKAAHPQWSPSAIKSALMTTAYTQDNTNSSLRDAAGGGFSN 600

Query: 601 PWAHGAGHVDPHKALSPGLVYDISTDDYIDFLCSLDYGIDHVKAIVKRSNITCSRKFADP 660
           PWAHGAGHVDPHKALSPGL+YDIST+DYI FLCSLDYGIDHV+AIVKRSNITCSRKFADP
Sbjct: 601 PWAHGAGHVDPHKALSPGLLYDISTNDYIAFLCSLDYGIDHVQAIVKRSNITCSRKFADP 660

Query: 661 GQLNYPSFSVVFGSKRVVRYTRKVTNVGAAGSVYEVSTTAPSVVNVIVKPSKLVFTKVGE 720
           GQLNYPSFSVVFGSKRVVRYTR VTNVGAAGSVY+V+TTAPSVV V VKPSKLVFTKVGE
Sbjct: 661 GQLNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVYDVATTAPSVVKVTVKPSKLVFTKVGE 720

Query: 721 RKRYTITFVASRDAARTTKFGFGSIAWTNEQHQVKSPVAFAWT 755
           RKRYT+TFVASRDAA+TT+FGFGSI W+N+QHQV+SPV+FAWT
Sbjct: 721 RKRYTVTFVASRDAAQTTRFGFGSIVWSNDQHQVRSPVSFAWT 762

BLAST of Sed0015954 vs. TAIR 10
Match: AT2G05920.1 (Subtilase family protein )

HSP 1 Score: 1073.5 bits (2775), Expect = 5.4e-314
Identity = 530/734 (72.21%), Postives = 620/734 (84.47%), Query Frame = 0

Query: 25  KTTYIVHMKHHALPSDYPTHLQSLSS--TASDSLLYTYTSAYHGFAASLDPDEAE-LLRQ 84
           K TYI+ + H   P  + TH    +S   +  SLLYTYT+++HGF+A LD  EA+ LL  
Sbjct: 27  KKTYIIRVNHSDKPESFLTHHDWYTSQLNSESSLLYTYTTSFHGFSAYLDSTEADSLLSS 86

Query: 85  SDSVLGVYEDTVYNLHTTRTPGFLGLDSDFGLWEGHNTQDLDQASHDVIIGVLDTGIWPE 144
           S+S+L ++ED +Y LHTTRTP FLGL+S+FG+       DL  +S+ VIIGVLDTG+WPE
Sbjct: 87  SNSILDIFEDPLYTLHTTRTPEFLGLNSEFGV------HDLGSSSNGVIIGVLDTGVWPE 146

Query: 145 SKSFDDVGMPEIPSRWRGECESGPDFSPSLCNKKLIGARSFSKGYQMASGGGYFKKPRED 204
           S+SFDD  MPEIPS+W+GECESG DF   LCNKKLIGARSFSKG+QMASGGG+  K RE 
Sbjct: 147 SRSFDDTDMPEIPSKWKGECESGSDFDSKLCNKKLIGARSFSKGFQMASGGGFSSK-RES 206

Query: 205 QSARDQDGHGTHTASTAAGSHVANASLLGYARGIARGMAPQARVAAYKTCWPTGCFGSDI 264
            S RD DGHGTHT++TAAGS V NAS LGYA G ARGMA +ARVA YK CW TGCFGSDI
Sbjct: 207 VSPRDVDGHGTHTSTTAAGSAVRNASFLGYAAGTARGMATRARVATYKVCWSTGCFGSDI 266

Query: 265 LAAMDRAILDGVDVLSLSLGGGSAPYYKDTIAIGAFAAMEKGVFVSCSAGNSGPNKASLA 324
           LAAMDRAILDGVDVLSLSLGGGSAPYY+DTIAIGAF+AME+GVFVSCSAGNSGP +AS+A
Sbjct: 267 LAAMDRAILDGVDVLSLSLGGGSAPYYRDTIAIGAFSAMERGVFVSCSAGNSGPTRASVA 326

Query: 325 NVAPWIMTVGAGTLDRDFPAYVDLGNGRRITGVSLYSGQGMGSKPVGLVYNKGSNSSSNV 384
           NVAPW+MTVGAGTLDRDFPA+ +LGNG+R+TGVSLYSG GMG+KP+ LVYNKG++SSSN+
Sbjct: 327 NVAPWVMTVGAGTLDRDFPAFANLGNGKRLTGVSLYSGVGMGTKPLELVYNKGNSSSSNL 386

Query: 385 CLPGSLEPALVRGKVVICDRGVNARVEKGGVVRDAGGIGMILANTAASGEELVADSHLLP 444
           CLPGSL+ ++VRGK+V+CDRGVNARVEKG VVRDAGG+GMI+ANTAASGEELVADSHLLP
Sbjct: 387 CLPGSLDSSIVRGKIVVCDRGVNARVEKGAVVRDAGGLGMIMANTAASGEELVADSHLLP 446

Query: 445 AVAVGRKVGDLIRQYVRSVSKPMAALSFGGTILNVRPSPVVAAFSSRGPNLVTPQILKPD 504
           A+AVG+K GDL+R+YV+S SKP A L F GT+L+V+PSPVVAAFSSRGPN VTP+ILKPD
Sbjct: 447 AIAVGKKTGDLLREYVKSDSKPTALLVFKGTVLDVKPSPVVAAFSSRGPNTVTPEILKPD 506

Query: 505 VIGPGVNILAAWSESVGPTGLDSDKRKTQFNIMSGTSMSCPHISGLAALLKAAHSEWSPS 564
           VIGPGVNILA WS+++GPTGLD D R+TQFNIMSGTSMSCPHISGLA LLKAAH EWSPS
Sbjct: 507 VIGPGVNILAGWSDAIGPTGLDKDSRRTQFNIMSGTSMSCPHISGLAGLLKAAHPEWSPS 566

Query: 565 AIKSALMTTAYTQDNTNSSLRDAAGGAFSNPWAHGAGHVDPHKALSPGLVYDISTDDYID 624
           AIKSALMTTAY  DNTN+ L DAA  + SNP+AHG+GHVDP KALSPGLVYDIST++YI 
Sbjct: 567 AIKSALMTTAYVLDNTNAPLHDAADNSLSNPYAHGSGHVDPQKALSPGLVYDISTEEYIR 626

Query: 625 FLCSLDYGIDHVKAIVKRSNITCSRKFADPGQLNYPSFSVVFGSKRVVRYTRKVTNVGAA 684
           FLCSLDY +DH+ AIVKR ++ CS+KF+DPGQLNYPSFSV+FG KRVVRYTR+VTNVGAA
Sbjct: 627 FLCSLDYTVDHIVAIVKRPSVNCSKKFSDPGQLNYPSFSVLFGGKRVVRYTREVTNVGAA 686

Query: 685 GSVYEVSTTAPSVVNVIVKPSKLVFTKVGERKRYTITFVASRDAARTTKFGFGSIAWTNE 744
            SVY+V+      V + VKPSKL F  VGE+KRYT+TFV+ +  + T K  FGSI W+N 
Sbjct: 687 SSVYKVTVNGAPSVGISVKPSKLSFKSVGEKKRYTVTFVSKKGVSMTNKAEFGSITWSNP 746

Query: 745 QHQVKSPVAFAWTR 756
           QH+V+SPVAF+W R
Sbjct: 747 QHEVRSPVAFSWNR 753

BLAST of Sed0015954 vs. TAIR 10
Match: AT5G67360.1 (Subtilase family protein )

HSP 1 Score: 781.9 bits (2018), Expect = 4.5e-226
Identity = 416/769 (54.10%), Postives = 535/769 (69.57%), Query Frame = 0

Query: 5   AKFLMASLLLLLPCVFI------NAKKTTYIVHMKHHALPSDYPTH-------LQSLSST 64
           + FL ++   LL C+        ++ + TYIVHM    +PS +  H       L+S+S +
Sbjct: 3   SSFLSSTAFFLLLCLGFCHVSSSSSDQGTYIVHMAKSQMPSSFDLHSNWYDSSLRSISDS 62

Query: 65  ASDSLLYTYTSAYHGFAASLDPDEAELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSDF 124
           A   LLYTY +A HGF+  L  +EA+ L     V+ V  +  Y LHTTRTP FLGLD   
Sbjct: 63  A--ELLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLD--- 122

Query: 125 GLWEGHNTQDL--DQASH-DVIIGVLDTGIWPESKSFDDVGMPEIPSRWRGECESGPDFS 184
                 +T DL  +  S+ DV++GVLDTG+WPESKS+ D G   IPS W+G CE+G +F+
Sbjct: 123 -----EHTADLFPEAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFT 182

Query: 185 PSLCNKKLIGARSFSKGYQMASGGGYFKKPREDQSARDQDGHGTHTASTAAGSHVANASL 244
            SLCN+KLIGAR F++GY+  S  G   + +E +S RD DGHGTHT+STAAGS V  ASL
Sbjct: 183 ASLCNRKLIGARFFARGYE--STMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASL 242

Query: 245 LGYARGIARGMAPQARVAAYKTCWPTGCFGSDILAAMDRAILDGVDVLSLSLGGGSAPYY 304
           LGYA G ARGMAP+ARVA YK CW  GCF SDILAA+D+AI D V+VLS+SLGGG + YY
Sbjct: 243 LGYASGTARGMAPRARVAVYKVCWLGGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYY 302

Query: 305 KDTIAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVDLGNG 364
           +D +AIGAFAAME+G+ VSCSAGN+GP+ +SL+NVAPWI TVGAGTLDRDFPA   LGNG
Sbjct: 303 RDGVAIGAFAAMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNG 362

Query: 365 RRITGVSLYSGQGMGSKPVGLVY--NKGSNSSSNVCLPGSLEPALVRGKVVICDRGVNAR 424
           +  TGVSL+ G+ +  K +  +Y  N  + ++ N+C+ G+L P  V+GK+V+CDRG+NAR
Sbjct: 363 KNFTGVSLFKGEALPDKLLPFIYAGNASNATNGNLCMTGTLIPEKVKGKIVMCDRGINAR 422

Query: 425 VEKGGVVRDAGGIGMILANTAASGEELVADSHLLPAVAVGRKVGDLIRQYVRSVSKPMAA 484
           V+KG VV+ AGG+GMILANTAA+GEELVAD+HLLPA  VG K GD+IR YV +   P A+
Sbjct: 423 VQKGDVVKAAGGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTAS 482

Query: 485 LSFGGTILNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESVGPTGLDSDK 544
           +S  GT++ V+PSPVVAAFSSRGPN +TP ILKPD+I PGVNILAAW+ + GPTGL SD 
Sbjct: 483 ISILGTVVGVKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDS 542

Query: 545 RKTQFNIMSGTSMSCPHISGLAALLKAAHSEWSPSAIKSALMTTAYTQDNTNSSLRDAAG 604
           R+ +FNI+SGTSMSCPH+SGLAALLK+ H EWSP+AI+SALMTTAY        L D A 
Sbjct: 543 RRVEFNIISGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIAT 602

Query: 605 GAFSNPWAHGAGHVDPHKALSPGLVYDISTDDYIDFLCSLDYGIDHVKAIVKRSNITCS- 664
           G  S P+ HGAGHV P  A +PGL+YD++T+DY+ FLC+L+Y    +++ V R N TC  
Sbjct: 603 GKPSTPFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRS-VSRRNYTCDP 662

Query: 665 RKFADPGQLNYPSFSVVFGSKRVVRYTRKVTNVGAAGSVYEVSTTAPSVVNVIVKPSKLV 724
            K      LNYPSF+V        +YTR VT+VG AG+     T+  + V + V+P+ L 
Sbjct: 663 SKSYSVADLNYPSFAVNVDGVGAYKYTRTVTSVGGAGTYSVKVTSETTGVKISVEPAVLN 722

Query: 725 FTKVGERKRYTITFVASRDAARTTKFGFGSIAWTNEQHQVKSPVAFAWT 755
           F +  E+K YT+TF         +   FGSI W++ +H V SPVA +WT
Sbjct: 723 FKEANEKKSYTVTFTVDSSKPSGSN-SFGSIEWSDGKHVVGSPVAISWT 757

BLAST of Sed0015954 vs. TAIR 10
Match: AT3G14067.1 (Subtilase family protein )

HSP 1 Score: 743.8 bits (1919), Expect = 1.4e-214
Identity = 412/777 (53.02%), Postives = 511/777 (65.77%), Query Frame = 0

Query: 4   MAKFLMASLLLLLP---CVFINAKKT-----TYIVHMKHHALPSDYPTH-------LQSL 63
           MAK  ++S+  + P   C F  +  +     +YIVH++    PS + +H       L+SL
Sbjct: 1   MAKLSLSSIFFVFPLLLCFFSPSSSSSDGLESYIVHVQRSHKPSLFSSHNNWHVSLLRSL 60

Query: 64  -SSTASDSLLYTYTSAYHGFAASLDPDEAELLRQSDSVLGVYEDTVYNLHTTRTPGFLGL 123
            SS    +LLY+Y+ A HGF+A L P +   LR+  SV+ V  D    +HTT TP FLG 
Sbjct: 61  PSSPQPATLLYSYSRAVHGFSARLSPIQTAALRRHPSVISVIPDQAREIHTTHTPAFLGF 120

Query: 124 DSDFGLWEGHNTQDLDQASHDVIIGVLDTGIWPESKSFDDVGMPEIPSRWRGECESGPDF 183
             + GLW   N         DVI+GVLDTGIWPE  SF D G+  IPS W+GECE GPDF
Sbjct: 121 SQNSGLWSNSN------YGEDVIVGVLDTGIWPEHPSFSDSGLGPIPSTWKGECEIGPDF 180

Query: 184 SPSLCNKKLIGARSFSKGYQMASGGGYFKKPREDQSARDQDGHGTHTASTAAGSHVANAS 243
             S CN+KLIGAR+F +GY     G      +E +S RD +GHGTHTASTAAGS VANAS
Sbjct: 181 PASSCNRKLIGARAFYRGYLTQRNGTKKHAAKESRSPRDTEGHGTHTASTAAGSVVANAS 240

Query: 244 LLGYARGIARGMAPQARVAAYKTCWPTGCFGSDILAAMDRAILDGVDVLSLSLG-GGSAP 303
           L  YARG A GMA +AR+AAYK CW  GC+ SDILAAMD+A+ DGV V+SLS+G  GSAP
Sbjct: 241 LYQYARGTATGMASKARIAAYKICWTGGCYDSDILAAMDQAVADGVHVISLSVGASGSAP 300

Query: 304 -YYKDTIAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVDL 363
            Y+ D+IAIGAF A   G+ VSCSAGNSGPN  +  N+APWI+TVGA T+DR+F A    
Sbjct: 301 EYHTDSIAIGAFGATRHGIVVSCSAGNSGPNPETATNIAPWILTVGASTVDREFAANAIT 360

Query: 364 GNGRRITGVSLYSGQGMGSKPVGLVYNKGSNSSSNVCLPGSLEPALVRGKVVICDRGVNA 423
           G+G+  TG SLY+G+ +    + LVY+   +  S +C PG L  +LV GK+V+CDRG NA
Sbjct: 361 GDGKVFTGTSLYAGESLPDSQLSLVYS--GDCGSRLCYPGKLNSSLVEGKIVLCDRGGNA 420

Query: 424 RVEKGGVVRDAGGIGMILANTAASGEELVADSHLLPAVAVGRKVGDLIRQYVRSVSKPMA 483
           RVEKG  V+ AGG GMILANTA SGEEL ADSHL+PA  VG K GD IR Y+++   P A
Sbjct: 421 RVEKGSAVKLAGGAGMILANTAESGEELTADSHLVPATMVGAKAGDQIRDYIKTSDSPTA 480

Query: 484 ALSFGGTILNVR-PSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESVGPTGLDS 543
            +SF GT++    PSP VAAFSSRGPN +TP ILKPDVI PGVNILA W+  VGPT LD 
Sbjct: 481 KISFLGTLIGPSPPSPRVAAFSSRGPNHLTPVILKPDVIAPGVNILAGWTGMVGPTDLDI 540

Query: 544 DKRKTQFNIMSGTSMSCPHISGLAALLKAAHSEWSPSAIKSALMTTAYTQDNTNSSLRDA 603
           D R+ QFNI+SGTSMSCPH+SGLAALL+ AH +WSP+AIKSAL+TTAY  +N+   + D 
Sbjct: 541 DPRRVQFNIISGTSMSCPHVSGLAALLRKAHPDWSPAAIKSALVTTAYDVENSGEPIEDL 600

Query: 604 AGGAFSNPWAHGAGHVDPHKALSPGLVYDISTDDYIDFLCSLDYGIDHVKAIVKRSNI-- 663
           A G  SN + HGAGHVDP+KAL+PGLVYDI   +Y+ FLC++ Y    +   ++   +  
Sbjct: 601 ATGKSSNSFIHGAGHVDPNKALNPGLVYDIEVKEYVAFLCAVGYEFPGILVFLQDPTLYD 660

Query: 664 TC-SRKFADPGQLNYPSFSVVFGSK-RVVRYTRKVTNVGA-AGSVYEVSTTAPSVVNVIV 723
            C + K    G LNYPSFSVVF S   VV+Y R V NVG+   +VYEV   +P+ V + V
Sbjct: 661 ACETSKLRTAGDLNYPSFSVVFASTGEVVKYKRVVKNVGSNVDAVYEVGVKSPANVEIDV 720

Query: 724 KPSKLVFTKVGERKRYTITF---VASRDAARTTKFGFGSIAWTNEQHQVKSPVAFAW 754
            PSKL F+K      Y +TF   V            FGSI WT+ +H VKSPVA  W
Sbjct: 721 SPSKLAFSKEKSVLEYEVTFKSVVLGGGVGSVPGHEFGSIEWTDGEHVVKSPVAVQW 769

BLAST of Sed0015954 vs. TAIR 10
Match: AT5G51750.1 (subtilase 1.3 )

HSP 1 Score: 732.6 bits (1890), Expect = 3.1e-211
Identity = 391/772 (50.65%), Postives = 507/772 (65.67%), Query Frame = 0

Query: 12  LLLLLPCVFINAK-------KTTYIVHMKHHALPSDYPTHLQSLSSTA------------ 71
           ++L +  +F+ A+       K TY++HM   A+P  Y  HLQ  SS              
Sbjct: 14  IILSINLIFLQAETTTQISTKKTYVIHMDKSAMPLPYTNHLQWYSSKINSVTQHKSQEEE 73

Query: 72  --SDSLLYTYTSAYHGFAASLDPDEAELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSD 131
             ++ +LYTY +A+HG AA L  +EAE L + D V+ V  +T Y LHTTR+P FLGL+  
Sbjct: 74  GNNNRILYTYQTAFHGLAAQLTQEEAERLEEEDGVVAVIPETRYELHTTRSPTFLGLERQ 133

Query: 132 FGLWEGHNTQDLDQASHDVIIGVLDTGIWPESKSFDDVGMPEIPSRWRGECESGPDFSPS 191
               E           HDV++GVLDTGIWPES+SF+D GM  +P+ WRG CE+G  F   
Sbjct: 134 ----ESERVWAERVTDHDVVVGVLDTGIWPESESFNDTGMSPVPATWRGACETGKRFLKR 193

Query: 192 LCNKKLIGARSFSKGYQMASGGGYFKKPREDQSARDQDGHGTHTASTAAGSHVANASLLG 251
            CN+K++GAR F +GY+ A+  G   +  E +S RD+DGHGTHTA+T AGS V  A+L G
Sbjct: 194 NCNRKIVGARVFYRGYEAAT--GKIDEELEYKSPRDRDGHGTHTAATVAGSPVKGANLFG 253

Query: 252 YARGIARGMAPQARVAAYKTCWPTGCFGSDILAAMDRAILDGVDVLSLSLGGGSAPYYKD 311
           +A G ARGMA +ARVAAYK CW  GCF SDIL+A+D+A+ DGV VLS+SLGGG + Y +D
Sbjct: 254 FAYGTARGMAQKARVAAYKVCWVGGCFSSDILSAVDQAVADGVQVLSISLGGGVSTYSRD 313

Query: 312 TIAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVDLGNGRR 371
           +++I  F AME GVFVSCSAGN GP+  SL NV+PWI TVGA T+DRDFPA V +G  R 
Sbjct: 314 SLSIATFGAMEMGVFVSCSAGNGGPDPISLTNVSPWITTVGASTMDRDFPATVKIGTMRT 373

Query: 372 ITGVSLYSGQGM--GSKPVGLVY---NKGSNSSSNVCLPGSLEPALVRGKVVICDRGVNA 431
             GVSLY G+ +   +K   LVY   N  S   ++ CL G+L+   V GK+VICDRGV  
Sbjct: 374 FKGVSLYKGRTVLPKNKQYPLVYLGRNASSPDPTSFCLDGALDRRHVAGKIVICDRGVTP 433

Query: 432 RVEKGGVVRDAGGIGMILANTAASGEELVADSHLLPAVAVGRKVGDLIRQYVRSVSKPMA 491
           RV+KG VV+ AGGIGM+L NTA +GEELVADSH+LPAVAVG K G LI+QY  +  K  A
Sbjct: 434 RVQKGQVVKRAGGIGMVLTNTATNGEELVADSHMLPAVAVGEKEGKLIKQYAMTSKKATA 493

Query: 492 ALSFGGTILNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESVGPTGLDSD 551
           +L   GT + ++PSPVVAAFSSRGPN ++ +ILKPD++ PGVNILAAW+  + P+ L SD
Sbjct: 494 SLEILGTRIGIKPSPVVAAFSSRGPNFLSLEILKPDLLAPGVNILAAWTGDMAPSSLSSD 553

Query: 552 KRKTQFNIMSGTSMSCPHISGLAALLKAAHSEWSPSAIKSALMTTAYTQDNTNSSLRDAA 611
            R+ +FNI+SGTSMSCPH+SG+AAL+K+ H +WSP+AIKSALMTTAY  DN    L DA+
Sbjct: 554 PRRVKFNILSGTSMSCPHVSGVAALIKSRHPDWSPAAIKSALMTTAYVHDNMFKPLTDAS 613

Query: 612 GGAFSNPWAHGAGHVDPHKALSPGLVYDISTDDYIDFLCSLDYGIDHVKAIVKRSNITCS 671
           G A S+P+ HGAGH+DP +A  PGLVYDI   +Y +FLC+ D     +K   K SN TC 
Sbjct: 614 GAAPSSPYDHGAGHIDPLRATDPGLVYDIGPQEYFEFLCTQDLSPSQLKVFTKHSNRTCK 673

Query: 672 RKFA-DPGQLNYPSFSVVFGSK---RVVRYTRKVTNVGAAGSVYEVSTTAPSVVNVIVKP 731
              A +PG LNYP+ S +F      + +   R VTNVG   S Y+VS +     +V V+P
Sbjct: 674 HTLAKNPGNLNYPAISALFPENTHVKAMTLRRTVTNVGPHISSYKVSVSPFKGASVTVQP 733

Query: 732 SKLVFTKVGERKRYTITFVASRDAARTTKFGFGSIAWTNEQHQVKSPVAFAW 754
             L FT   ++  YT+TF   R   R  +  FG + W +  H+V+SPV   W
Sbjct: 734 KTLNFTSKHQKLSYTVTF---RTRFRMKRPEFGGLVWKSTTHKVRSPVIITW 776

BLAST of Sed0015954 vs. TAIR 10
Match: AT4G34980.1 (subtilisin-like serine protease 2 )

HSP 1 Score: 718.8 bits (1854), Expect = 4.7e-207
Identity = 386/757 (50.99%), Postives = 519/757 (68.56%), Query Frame = 0

Query: 12  LLLLLPCVFINAKKT----TYIVHMKHHALPSDYPTHLQSLSSTASDS--LLYTYTSAYH 71
           LLL L   FI+   +    T+I  +   ++PS +PTH    S+  ++   +++ Y + +H
Sbjct: 8   LLLFLSFPFISFAASQAAKTFIFRIDGGSMPSIFPTHYHWYSTEFAEESRIVHVYHTVFH 67

Query: 72  GFAASLDPDEAELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSDFGLWEGHNTQDLDQA 131
           GF+A + PDEA+ LR   +VL V+ED    LHTTR+P FLGL +  GLW      + D  
Sbjct: 68  GFSAVVTPDEADNLRNHPAVLAVFEDRRRELHTTRSPQFLGLQNQKGLW-----SESDYG 127

Query: 132 SHDVIIGVLDTGIWPESKSFDDVGMPEIPSRWRGECESGPDFSPSLCNKKLIGARSFSKG 191
           S DVIIGV DTGIWPE +SF D+ +  IP RWRG CESG  FSP  CN+K+IGAR F+KG
Sbjct: 128 S-DVIIGVFDTGIWPERRSFSDLNLGPIPKRWRGVCESGARFSPRNCNRKIIGARFFAKG 187

Query: 192 YQMASGGGYFKKPREDQSARDQDGHGTHTASTAAGSHVANASLLGYARGIARGMAPQARV 251
            Q A  GG   K  E  S RD DGHGTHT+STAAG H   AS+ GYA G+A+G+AP+AR+
Sbjct: 188 QQAAVIGG-INKTVEFLSPRDADGHGTHTSSTAAGRHAFKASMSGYASGVAKGVAPKARI 247

Query: 252 AAYKTCW-PTGCFGSDILAAMDRAILDGVDVLSLSLGGG---SAPYYKDTIAIGAFAAME 311
           AAYK CW  +GC  SDILAA D A+ DGVDV+S+S+GGG   ++PYY D IAIG++ A  
Sbjct: 248 AAYKVCWKDSGCLDSDILAAFDAAVRDGVDVISISIGGGDGITSPYYLDPIAIGSYGAAS 307

Query: 312 KGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVDLGNGRRITGVSLYSGQG 371
           KG+FVS SAGN GPN  S+ N+APW+ TVGA T+DR+FPA   LG+G R+ GVSLY+G  
Sbjct: 308 KGIFVSSSAGNEGPNGMSVTNLAPWVTTVGASTIDRNFPADAILGDGHRLRGVSLYAGVP 367

Query: 372 MGSKPVGLVY-NKGSNSSSNVCLPGSLEPALVRGKVVICDRGVNARVEKGGVVRDAGGIG 431
           +  +   +VY  K   SS+++C+  +L+P  VRGK+VICDRG + RV KG VV+ AGG+G
Sbjct: 368 LNGRMFPVVYPGKSGMSSASLCMENTLDPKQVRGKIVICDRGSSPRVAKGLVVKKAGGVG 427

Query: 432 MILANTAASGEELVADSHLLPAVAVGRKVGDLIRQYVRSVSKPMAALSFGGTILNVRPSP 491
           MILAN A++GE LV D+HL+PA AVG   GD I+ Y  S   P+A++ F GTI+ ++P+P
Sbjct: 428 MILANGASNGEGLVGDAHLIPACAVGSNEGDRIKAYASSHPNPIASIDFRGTIVGIKPAP 487

Query: 492 VVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESVGPTGLDSDKRKTQFNIMSGTSMS 551
           V+A+FS RGPN ++P+ILKPD+I PGVNILAAW+++VGPTGL SD RKT+FNI+SGTSM+
Sbjct: 488 VIASFSGRGPNGLSPEILKPDLIAPGVNILAAWTDAVGPTGLPSDPRKTEFNILSGTSMA 547

Query: 552 CPHISGLAALLKAAHSEWSPSAIKSALMTTAYTQDNTNSSLRDAAGGAFSNPWAHGAGHV 611
           CPH+SG AALLK+AH +WSP+ I+SA+MTT    DN+N SL D + G  + P+ +G+GH+
Sbjct: 548 CPHVSGAAALLKSAHPDWSPAVIRSAMMTTTNLVDNSNRSLIDESTGKSATPYDYGSGHL 607

Query: 612 DPHKALSPGLVYDISTDDYIDFLCSLDYGIDHVKAIVKRSNITC-SRKFADPGQLNYPSF 671
           +  +A++PGLVYDI+ DDYI FLCS+ YG   ++ ++ R+ + C + +   PG LNYPS 
Sbjct: 608 NLGRAMNPGLVYDITNDDYITFLCSIGYGPKTIQ-VITRTPVRCPTTRKPSPGNLNYPSI 667

Query: 672 SVVFGSKR--VVRYT--RKVTNVGAAGSVYEVSTTAPSVVNVIVKPSKLVFTKVGERKRY 731
           + VF + R  +V  T  R  TNVG A +VY     +P  V V VKP +LVFT   +R+ Y
Sbjct: 668 TAVFPTNRRGLVSKTVIRTATNVGQAEAVYRARIESPRGVTVTVKPPRLVFTSAVKRRSY 727

Query: 732 TITFVASRDAARTTKFG--FGSIAW-TNEQHQVKSPV 750
            +T   +       + G  FGS+ W    +H V+SP+
Sbjct: 728 AVTVTVNTRNVVLGETGAVFGSVTWFDGGKHVVRSPI 756