Sed0015551 (gene) Chayote v1

Overview
NameSed0015551
Typegene
OrganismSechium edule (Chayote v1)
Description2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein
LocationLG12: 33384393 .. 33386670 (+)
RNA-Seq ExpressionSed0015551
SyntenySed0015551
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTTCAATCACCGTCGCCGATGAAGTTCCGGCGAGATTTATCCATACAGAAAAAAATAGAGGAGTTTTAGAAGCTCCTTTAATGGAAGCTCCTGTAATTGATGTTTCTCTGCTTTCTTCTTCCTCCGATTCCGGGCCGGAGCTCGATAGACTCCGACATGGGCTTCACCACTGGGGCTGCTTTCTGGTTTAATCTCATATTTCTTTCAAAATTTATGTCTACATTTATTACTTAAATTTTTTTAATCACATTTGAAATTTTATATAGAATTACCATCACCGGCACATTTTACCTTTGTATTATATTTGGAGTAAAATTTTATAAATTATGAGTCTATCATTAATCGAATTCTATCGCAAATAAAACTCAATTTAAATTTTTTGGTACAAATGAATTAATTTTGATTTGTGCTGAAAATATTTTGCATTTTTTTTGTTATACATTCAAAATCCCTTATATATTATTTTTATAACATAATAAAAGCAAAAAGGTTTAAGTGTTTCTCAAAATTTATTACGATATAAGAGTCACTAAATTAGAAATTGTTGTAGTTCATATAATGCAATTCGAAGTTCGTATACGTTTAACGTTTTGTACAGTCATTATAGATTGATCAATGATTCAATAAATTCTTGGTGATTAGTGTACTGCATTAGAGTCTGTAAATTCAACATTGTGTTTGTGAGTGATATCAATCGATGACAGTTCATGAGTGGTGCTTGTTCGTGCAAGTGTAGTGTATCTGACATTATGTATTACCGGTATAGATTGTTCATAAGTTGTTCGGTATTGTTCGACCTAATCGATAACAGATGTAGTAGTCATGTATGAGACGAATATGTCAATGACTTTGTTTTCAAGTTGGTCGTTTGTAATTTACATGCTGTTACCAAGTTTTATTTAGCTATTTTATAGAATTTATTAGGATAGATTATATATTTTGTCAAAGTTAATGAAAATGGGGTAACTTAATTAAAGTTTTGTGGGGTGTTACAGGCGATATATCATGGGATGTCAACAGAATTTCTAGAAGAAGTTCGACAAGTAGTGAAACAATTTTTCGCACTTCCAATGGACGACAAACAGAAACACTTGAGAGACGAAAACGGCATCGAAGGATATGGAAACGACATGATTCTATCCGAGAACCAGATTCTGGATTGGAATGATCGTTTGATCCTCACATTGCATCCACAACAGAGTCGCCGTTTGAAGTATTGGCCAACAAAGCCTCCAAGATTCAGGGAAGTTGTTCTTGAGTACACTGAAAATGTGAGGCTCATAAGTGAAGTAATCTTGAAGGCTATGGCCAAGTCATTGGATTTGGATGAGAATTGCTTTCTTAAGCAGTATGGTGAGCAATTTACACTCCCTGCACGGTTCAACTTCTACCCGAAATGTCGAAATCCCAAGCTCGTTCTCGGTCAGAAGCCGCACTCTGATGTATCGGCGATCACGCTTCTGTTGCTGGACAAGGAAGCTGGAGGTCTTCAGTTCTTGAGAGGCAATGAGTGGTTTAACGCACCGGTTGGTTCCGATGATGCTCTTCTTGTCAGCCTCGGAGACCAAGCAGAGGTAACTCAAAAACACTTATTTAGAATTTGGGCATCCAATTTAAGAATAATGAACTCAAAGAGGCACGTACGAATCTCCATTGGATTGTAGTATAGACGAGTTGCTTCGCTTTGCTCTAAAACCTTATTTCTTCCCATCATAATATCTACGAGCTCATTTAATCAAATTCACAGAATGTCTTTGGAACTCTTTTATTTAGGTAGCTTTCGTGTCTTATTTTTCGGGTTCCAAACCCCCCTTTCTCTATAAGGAAAGTACATATCCATGCCTAGGGATATATACAAGAATCATTCTTTAGATAAATCGTATTATCTAATATAATATCAGAGTCTATCAAACTCGAATAGTTATTTTGTCCAATAAAAATGAAATTCTGATTTGATTAAAAAAAGCACCATCTTGAAAAGGCCACTTGAAACGATAGTATATAATTATCAAAATGGGATGAAAATTGGTTTTGCAGGTAACAAGCAATGGGAAATTCAAGAGTCCAGTTCATAGGGTATTGATAAACTCAGAGAGGGAAAGGGTATCTTTAGCAGTGTTTTACCTTCCAGATTCAGAGAAAGAAATAGAGCCACTTGAGGAGCTAATCAATGAAACTCAGCCAAGGATGTACAAGACTGTCATGAACTTGCCTGCCCTTTACTTTCAATACTACCAGCAGGGAAGGAGAACAATAGAGGCTGCAATAATCTAA

mRNA sequence

ATGGCTTCAATCACCGTCGCCGATGAAGTTCCGGCGAGATTTATCCATACAGAAAAAAATAGAGGAGTTTTAGAAGCTCCTTTAATGGAAGCTCCTGTAATTGATGTTTCTCTGCTTTCTTCTTCCTCCGATTCCGGGCCGGAGCTCGATAGACTCCGACATGGGCTTCACCACTGGGGCTGCTTTCTGGCGATATATCATGGGATGTCAACAGAATTTCTAGAAGAAGTTCGACAAGTAGTGAAACAATTTTTCGCACTTCCAATGGACGACAAACAGAAACACTTGAGAGACGAAAACGGCATCGAAGGATATGGAAACGACATGATTCTATCCGAGAACCAGATTCTGGATTGGAATGATCGTTTGATCCTCACATTGCATCCACAACAGAGTCGCCGTTTGAAGGAAGTTGTTCTTGAGTACACTGAAAATGTGAGGCTCATAAGTGAAGTAATCTTGAAGGCTATGGCCAAGTCATTGGATTTGGATGAGAATTGCTTTCTTAAGCAGTATGGTGAGCAATTTACACTCCCTGCACGGTTCAACTTCTACCCGAAATGTCGAAATCCCAAGCTCGTTCTCGGTCAGAAGCCGCACTCTGATGTATCGGCGATCACGCTTCTGTTGCTGGACAAGGAAGCTGGAGGTCTTCAGTTCTTGAGAGGCAATGAGTGGTTTAACGCACCGGTTGGTTCCGATGATGCTCTTCTTGTCAGCCTCGGAGACCAAGCAGAGGTAACAAGCAATGGGAAATTCAAGAGTCCAGTTCATAGGGTATTGATAAACTCAGAGAGGGAAAGGGTATCTTTAGCAGTGTTTTACCTTCCAGATTCAGAGAAAGAAATAGAGCCACTTGAGGAGCTAATCAATGAAACTCAGCCAAGGATGTACAAGACTGTCATGAACTTGCCTGCCCTTTACTTTCAATACTACCAGCAGGGAAGGAGAACAATAGAGGCTGCAATAATCTAA

Coding sequence (CDS)

ATGGCTTCAATCACCGTCGCCGATGAAGTTCCGGCGAGATTTATCCATACAGAAAAAAATAGAGGAGTTTTAGAAGCTCCTTTAATGGAAGCTCCTGTAATTGATGTTTCTCTGCTTTCTTCTTCCTCCGATTCCGGGCCGGAGCTCGATAGACTCCGACATGGGCTTCACCACTGGGGCTGCTTTCTGGCGATATATCATGGGATGTCAACAGAATTTCTAGAAGAAGTTCGACAAGTAGTGAAACAATTTTTCGCACTTCCAATGGACGACAAACAGAAACACTTGAGAGACGAAAACGGCATCGAAGGATATGGAAACGACATGATTCTATCCGAGAACCAGATTCTGGATTGGAATGATCGTTTGATCCTCACATTGCATCCACAACAGAGTCGCCGTTTGAAGGAAGTTGTTCTTGAGTACACTGAAAATGTGAGGCTCATAAGTGAAGTAATCTTGAAGGCTATGGCCAAGTCATTGGATTTGGATGAGAATTGCTTTCTTAAGCAGTATGGTGAGCAATTTACACTCCCTGCACGGTTCAACTTCTACCCGAAATGTCGAAATCCCAAGCTCGTTCTCGGTCAGAAGCCGCACTCTGATGTATCGGCGATCACGCTTCTGTTGCTGGACAAGGAAGCTGGAGGTCTTCAGTTCTTGAGAGGCAATGAGTGGTTTAACGCACCGGTTGGTTCCGATGATGCTCTTCTTGTCAGCCTCGGAGACCAAGCAGAGGTAACAAGCAATGGGAAATTCAAGAGTCCAGTTCATAGGGTATTGATAAACTCAGAGAGGGAAAGGGTATCTTTAGCAGTGTTTTACCTTCCAGATTCAGAGAAAGAAATAGAGCCACTTGAGGAGCTAATCAATGAAACTCAGCCAAGGATGTACAAGACTGTCATGAACTTGCCTGCCCTTTACTTTCAATACTACCAGCAGGGAAGGAGAACAATAGAGGCTGCAATAATCTAA

Protein sequence

MASITVADEVPARFIHTEKNRGVLEAPLMEAPVIDVSLLSSSSDSGPELDRLRHGLHHWGCFLAIYHGMSTEFLEEVRQVVKQFFALPMDDKQKHLRDENGIEGYGNDMILSENQILDWNDRLILTLHPQQSRRLKEVVLEYTENVRLISEVILKAMAKSLDLDENCFLKQYGEQFTLPARFNFYPKCRNPKLVLGQKPHSDVSAITLLLLDKEAGGLQFLRGNEWFNAPVGSDDALLVSLGDQAEVTSNGKFKSPVHRVLINSERERVSLAVFYLPDSEKEIEPLEELINETQPRMYKTVMNLPALYFQYYQQGRRTIEAAII
Homology
BLAST of Sed0015551 vs. NCBI nr
Match: KAA0050082.1 (protein SRG1-like [Cucumis melo var. makuwa])

HSP 1 Score: 457.6 bits (1176), Expect = 8.9e-125
Identity = 226/326 (69.33%), Postives = 266/326 (81.60%), Query Frame = 0

Query: 9   EVPARFIHTEKNRGVLEAPLMEAPVIDVSLLSSSSDSGPELDRLRHGLHHWGCFLAIYHG 68
           EVP R+IH E +RG   APLM APVID++LLSSSS SGPEL++LRHGL  WGCF AI HG
Sbjct: 37  EVPERYIHKESDRGARNAPLMAAPVIDIALLSSSSKSGPELEKLRHGLQSWGCFQAINHG 96

Query: 69  MSTEFLEEVRQVVKQFFALPMDDKQKHLRDENGIEGYGNDMILSENQILDWNDRLILTLH 128
           M++E+L+EVRQ+ KQFF L M++K K+L++E+ +EGYGNDMILS  QILDW DRL LT++
Sbjct: 97  MTSEYLDEVRQLTKQFFGLSMEEKLKYLKEEHEMEGYGNDMILSNQQILDWTDRLYLTVY 156

Query: 129 PQQSRRLK----------EVVLEYTENVRLISEVILKAMAKSLDLDENCFLKQYGEQFTL 188
           P QSRR K          EVV EYT NV+LISE ILKAMA+SLDLDE+ FL QYGEQ  L
Sbjct: 157 PHQSRRFKYWPTNPQRFREVVGEYTTNVKLISEKILKAMARSLDLDESSFLNQYGEQVKL 216

Query: 189 PARFNFYPKCRNPKLVLGQKPHSDVSAITLLLLDKEAGGLQFLRGNEWFNAPVGSDDALL 248
            ARFNFYP+CRNP LVLG KPH+D SAIT+LL DKE  GLQF++ NEW+NAP+   DALL
Sbjct: 217 DARFNFYPRCRNPDLVLGVKPHADGSAITILLQDKEVEGLQFMKDNEWYNAPI-VPDALL 276

Query: 249 VSLGDQAEVTSNGKFKSPVHRVLINSERERVSLAVFYLPDSEKEIEPLEELINETQPRMY 308
           V++GDQ E+TSNG FKSPVHRVL NSERER+SLAVFYLPDSEKEIEPLEELINE+QPR+Y
Sbjct: 277 VNVGDQVEITSNGIFKSPVHRVLTNSERERISLAVFYLPDSEKEIEPLEELINESQPRLY 336

Query: 309 KTVMNLPALYFQYYQQGRRTIEAAII 325
           K+V N   LYF+YYQQG+R +EAA I
Sbjct: 337 KSVKNFVGLYFEYYQQGQRPMEAARI 361

BLAST of Sed0015551 vs. NCBI nr
Match: TYK10346.1 (protein SRG1-like [Cucumis melo var. makuwa])

HSP 1 Score: 457.2 bits (1175), Expect = 1.2e-124
Identity = 226/326 (69.33%), Postives = 266/326 (81.60%), Query Frame = 0

Query: 9   EVPARFIHTEKNRGVLEAPLMEAPVIDVSLLSSSSDSGPELDRLRHGLHHWGCFLAIYHG 68
           EVP R+IH E +RG   APLM APVID++LLSSSS SGPEL++LRHGL  WGCF AI HG
Sbjct: 37  EVPERYIHKESDRGARNAPLMAAPVIDIALLSSSSKSGPELEKLRHGLQSWGCFQAINHG 96

Query: 69  MSTEFLEEVRQVVKQFFALPMDDKQKHLRDENGIEGYGNDMILSENQILDWNDRLILTLH 128
           M++E+L+EVRQ+ KQFF L M++K K+L++E+ +EGYGNDMILS  QILDW DRL LT++
Sbjct: 97  MTSEYLDEVRQLTKQFFGLSMEEKLKYLKEEHEMEGYGNDMILSNQQILDWTDRLYLTVY 156

Query: 129 PQQSRRLK----------EVVLEYTENVRLISEVILKAMAKSLDLDENCFLKQYGEQFTL 188
           P QSRR K          EVV EYT NV+LISE ILKAMA+SLDLDE+ FL QYGEQ  L
Sbjct: 157 PHQSRRFKYWPINPQRFREVVGEYTTNVKLISEKILKAMARSLDLDESSFLNQYGEQVKL 216

Query: 189 PARFNFYPKCRNPKLVLGQKPHSDVSAITLLLLDKEAGGLQFLRGNEWFNAPVGSDDALL 248
            ARFNFYP+CRNP LVLG KPH+D SAIT+LL DKE  GLQF++ NEW+NAP+ + DALL
Sbjct: 217 DARFNFYPRCRNPDLVLGVKPHADGSAITILLQDKEVEGLQFMKDNEWYNAPIVT-DALL 276

Query: 249 VSLGDQAEVTSNGKFKSPVHRVLINSERERVSLAVFYLPDSEKEIEPLEELINETQPRMY 308
           V++GDQ E+TSNG FKSPVHRVL NSERER+SLAVFYLPDSEKEIEPLEELINE+QPR+Y
Sbjct: 277 VNVGDQVEITSNGIFKSPVHRVLTNSERERISLAVFYLPDSEKEIEPLEELINESQPRLY 336

Query: 309 KTVMNLPALYFQYYQQGRRTIEAAII 325
           K+V N   LYF+YYQQG R +EAA I
Sbjct: 337 KSVKNFVGLYFEYYQQGERPMEAARI 361

BLAST of Sed0015551 vs. NCBI nr
Match: XP_008465535.1 (PREDICTED: protein SRG1-like [Cucumis melo] >XP_016903407.1 PREDICTED: protein SRG1-like [Cucumis melo])

HSP 1 Score: 455.7 bits (1171), Expect = 3.4e-124
Identity = 225/326 (69.02%), Postives = 266/326 (81.60%), Query Frame = 0

Query: 9   EVPARFIHTEKNRGVLEAPLMEAPVIDVSLLSSSSDSGPELDRLRHGLHHWGCFLAIYHG 68
           EVP R+IH E +RG   APLM APVID++LLSSSS SGPEL++LRHGL  WGCF AI HG
Sbjct: 37  EVPERYIHKESDRGARNAPLMAAPVIDIALLSSSSKSGPELEKLRHGLQSWGCFQAINHG 96

Query: 69  MSTEFLEEVRQVVKQFFALPMDDKQKHLRDENGIEGYGNDMILSENQILDWNDRLILTLH 128
           M++E+L+EVRQ+ KQFF L M++K K+L++E+ +EGYGNDMILS  QILDW DRL LT++
Sbjct: 97  MTSEYLDEVRQLTKQFFGLSMEEKLKYLKEEHEMEGYGNDMILSNQQILDWTDRLYLTVY 156

Query: 129 PQQSRRLK----------EVVLEYTENVRLISEVILKAMAKSLDLDENCFLKQYGEQFTL 188
           P QSRR K          EVV EYT NV+LISE ILKAMA+SLDLDE+ FL QYGEQ  L
Sbjct: 157 PHQSRRFKYWPTNPQRFREVVGEYTTNVKLISEKILKAMARSLDLDESSFLSQYGEQVKL 216

Query: 189 PARFNFYPKCRNPKLVLGQKPHSDVSAITLLLLDKEAGGLQFLRGNEWFNAPVGSDDALL 248
            ARFNFYP+CRNP LVLG KPH+D SAIT+LL DK+  GLQF++ NEW+NAP+ + DALL
Sbjct: 217 DARFNFYPRCRNPDLVLGVKPHADGSAITILLQDKKVEGLQFMKDNEWYNAPIVT-DALL 276

Query: 249 VSLGDQAEVTSNGKFKSPVHRVLINSERERVSLAVFYLPDSEKEIEPLEELINETQPRMY 308
           V++GDQ E+TSNG FKSPVHRVL NSERER+SLAVFYLPDSEKEIEPLEELINE+QPR+Y
Sbjct: 277 VNVGDQVEITSNGIFKSPVHRVLTNSERERISLAVFYLPDSEKEIEPLEELINESQPRLY 336

Query: 309 KTVMNLPALYFQYYQQGRRTIEAAII 325
           K+V N   LYF+YYQQG R +EAA I
Sbjct: 337 KSVKNFVGLYFEYYQQGERPMEAARI 361

BLAST of Sed0015551 vs. NCBI nr
Match: XP_022134811.1 (uncharacterized protein LOC111006992 [Momordica charantia])

HSP 1 Score: 453.4 bits (1165), Expect = 1.7e-123
Identity = 226/326 (69.33%), Postives = 263/326 (80.67%), Query Frame = 0

Query: 9   EVPARFIHTEKNRGVLEAPLMEAPVIDVSLLSSSSDSGPELDRLRHGLHHWGCFLAIYHG 68
           EVP R+IH E +RG L+APLMEAPVID+ LLSS S++GPEL++LRHGLH WGCF AI HG
Sbjct: 18  EVPERYIHKECDRGALDAPLMEAPVIDIGLLSSPSNTGPELEKLRHGLHSWGCFQAINHG 77

Query: 69  MSTEFLEEVRQVVKQFFALPMDDKQKHLRDENGIEGYGNDMILSENQILDWNDRLILTLH 128
           MS EFLEEVRQV K FFALPM+DK KH R+E+ +EGYGNDMI S  QILDW DRL LT+ 
Sbjct: 78  MSPEFLEEVRQVAKLFFALPMEDKLKHSREEDKMEGYGNDMIFSNQQILDWTDRLYLTVC 137

Query: 129 PQQSRRLK----------EVVLEYTENVRLISEVILKAMAKSLDLDENCFLKQYGEQFTL 188
           P++SRR K          EV+ EYT NV+L+SE ILKAMA+SLDLDEN FL QYG++  L
Sbjct: 138 PEESRRFKYWPTNPERFREVLHEYTANVKLLSEKILKAMARSLDLDENSFLNQYGKRVQL 197

Query: 189 PARFNFYPKCRNPKLVLGQKPHSDVSAITLLLLDKEAGGLQFLRGNEWFNAPVGSDDALL 248
            ARFNFY +CRNP LVLG KPH+D SAIT+LL DKE  GLQFL+GNEW+NAP+   DALL
Sbjct: 198 DARFNFYLRCRNPNLVLGVKPHADGSAITILLQDKEVEGLQFLKGNEWWNAPI-IPDALL 257

Query: 249 VSLGDQAEVTSNGKFKSPVHRVLINSERERVSLAVFYLPDSEKEIEPLEELINETQPRMY 308
           V++GDQ E+TSNG FKS VHRVL NSERER+SLAVFYLPD +KEIEPLE+LINET PR+Y
Sbjct: 258 VNVGDQGEITSNGIFKSRVHRVLTNSERERISLAVFYLPDPQKEIEPLEKLINETHPRLY 317

Query: 309 KTVMNLPALYFQYYQQGRRTIEAAII 325
           +TV N   L+FQYYQQG+R  EAA I
Sbjct: 318 RTVKNFVGLFFQYYQQGQRPREAAKI 342

BLAST of Sed0015551 vs. NCBI nr
Match: XP_022987763.1 (probable 2-oxoglutarate-dependent dioxygenase ANS isoform X2 [Cucurbita maxima])

HSP 1 Score: 452.2 bits (1162), Expect = 3.7e-123
Identity = 223/326 (68.40%), Postives = 265/326 (81.29%), Query Frame = 0

Query: 9   EVPARFIHTEKNRGVLEAPLMEAPVIDVSLLSSSSDSGPELDRLRHGLHHWGCFLAIYHG 68
           EVP R+IH E +RG L+APLM APVID++LLSS S SGPEL++LRHGL  WGCF  + HG
Sbjct: 18  EVPERYIHIESDRGALDAPLMAAPVIDMALLSSPSKSGPELEKLRHGLQSWGCFQVVNHG 77

Query: 69  MSTEFLEEVRQVVKQFFALPMDDKQKHLRDENGIEGYGNDMILSENQILDWNDRLILTLH 128
           MS EFL+EVR++ KQFF LPM++K K+ R+EN IEGYGNDMILS  QILDW DRL LT++
Sbjct: 78  MSAEFLDEVRRLAKQFFDLPMEEKSKYSREENEIEGYGNDMILSNQQILDWTDRLYLTVY 137

Query: 129 PQQSRRLK----------EVVLEYTENVRLISEVILKAMAKSLDLDENCFLKQYGEQFTL 188
           P++S R K          EV+ EYT NV+L+SE ILKAMA SLDL+E+ F+KQYGE+  L
Sbjct: 138 PKESHRFKYWPTNPERFREVLHEYTANVKLLSEKILKAMAISLDLNEDSFIKQYGEEVKL 197

Query: 189 PARFNFYPKCRNPKLVLGQKPHSDVSAITLLLLDKEAGGLQFLRGNEWFNAPVGSDDALL 248
            ARFNFYP+CRNP LVLG KPH+D SAIT+LL D+E  GLQFL GNEWFNAP+    ALL
Sbjct: 198 DARFNFYPRCRNPDLVLGVKPHADGSAITILLQDEEVEGLQFLNGNEWFNAPI-VPGALL 257

Query: 249 VSLGDQAEVTSNGKFKSPVHRVLINSERERVSLAVFYLPDSEKEIEPLEELINETQPRMY 308
           V++GDQAE+TSNG FKSPVHRVL NSERER+SLAVFYLPDSEKEIEPLE+LI+ET+PR+Y
Sbjct: 258 VNVGDQAEITSNGIFKSPVHRVLTNSERERISLAVFYLPDSEKEIEPLEKLIDETRPRLY 317

Query: 309 KTVMNLPALYFQYYQQGRRTIEAAII 325
           K+V N   LYFQYYQQG+R +EAA I
Sbjct: 318 KSVKNFVGLYFQYYQQGQRPMEAARI 342

BLAST of Sed0015551 vs. ExPASy Swiss-Prot
Match: O80449 (Jasmonate-induced oxygenase 4 OS=Arabidopsis thaliana OX=3702 GN=JOX4 PE=1 SV=1)

HSP 1 Score: 189.5 bits (480), Expect = 5.9e-47
Identity = 108/313 (34.50%), Postives = 173/313 (55.27%), Query Frame = 0

Query: 3   SITVADEVPARFIHTEKNRGVLEAPLMEAPVIDVSLLSSSSDSG-PE-LDRLRHGLHHWG 62
           S T    VP R++     R V      +A  I++ +L  +   G PE L  +R     WG
Sbjct: 16  SQTGVPTVPNRYVKPAHQRPVFNTTQSDAG-IEIPVLDMNDVWGKPEGLRLVRSACEEWG 75

Query: 63  CFLAIYHGMSTEFLEEVRQVVKQFFALPMDDKQKHLRDENGIEGYGNDMILSENQILDWN 122
            F  + HG++   +E VR   ++FF LP+++K+K+    +  EGYG+ + + ++  LDW+
Sbjct: 76  FFQMVNHGVTHSLMERVRGAWREFFELPLEEKRKYANSPDTYEGYGSRLGVVKDAKLDWS 135

Query: 123 DRLIL----------TLHPQQSRRLKEVVLEYTENVRLISEVILKAMAKSLDLDENCFLK 182
           D   L          +  P Q  +++E++ +Y E VR + E + + +++SL L  N  ++
Sbjct: 136 DYFFLNYLPSSIRNPSKWPSQPPKIRELIEKYGEEVRKLCERLTETLSESLGLKPNKLMQ 195

Query: 183 QY--GEQFTLPARFNFYPKCRNPKLVLGQKPHSDVSAITLLLLDKEAGGLQFLRGNEWFN 242
               G++     R NFYPKC  P+L LG   HSD   IT+LL D++  GLQ  RG+ W  
Sbjct: 196 ALGGGDKVGASLRTNFYPKCPQPQLTLGLSSHSDPGGITILLPDEKVAGLQVRRGDGWVT 255

Query: 243 APVGSDDALLVSLGDQAEVTSNGKFKSPVHRVLINSERERVSLAVFYLPDSEKEIEPLEE 302
                 +AL+V++GDQ ++ SNG +KS  H+V++NS  ERVSLA FY P S+  + P+EE
Sbjct: 256 IK-SVPNALIVNIGDQLQILSNGIYKSVEHQVIVNSGMERVSLAFFYNPRSDIPVGPIEE 315

BLAST of Sed0015551 vs. ExPASy Swiss-Prot
Match: Q94LP4 (2-oxoglutarate-dependent dioxygenase 11 OS=Oryza sativa subsp. japonica OX=39947 GN=2ODD11 PE=1 SV=1)

HSP 1 Score: 181.8 bits (460), Expect = 1.2e-44
Identity = 106/330 (32.12%), Postives = 170/330 (51.52%), Query Frame = 0

Query: 8   DEVPARFIHTEKNRGVLEAPL---MEAPVIDVSLLSSSSDSGPELDRLRHGLHHWGCFLA 67
           + +P R+I  E +   +       M  P+ID+  L     S  E  +LR    +WG FL 
Sbjct: 27  EHIPERYIRPEASSEEVINNYQGDMAIPIIDLKKLLCPQSSEEECVKLRSACQYWGFFLL 86

Query: 68  IYHGMSTEFLEEVRQVVKQFFALPMDDKQKHLRDENGIEGYGNDMILSENQILDWNDRLI 127
           I HG+  E +  +++ +  FF+ P+D K+++ +  N +EGYG   + SE+Q LDW D L 
Sbjct: 87  INHGVPDEVIANLKRDIVDFFSQPLDTKKEYTQLPNSLEGYGQSFVFSEDQKLDWADMLY 146

Query: 128 LTLHPQQSRRL----------KEVVLEYTENVRLISEVILKAMAKSLDLDENCFLKQYGE 187
           L +HP  SR L          ++ +  Y+   + ++  + + MAK++       L  + E
Sbjct: 147 LHVHPSDSRDLRFWPTSPASFRQSIDAYSSETKSLALCLFEFMAKAVGAKPESLLDLFEE 206

Query: 188 QFTLPARFNFYPKCRNPKLVLGQKPHSDVSAITLLLLDKEAGGLQFLRGNEWF--NAPVG 247
           Q     R  +YP CR    V+G  PHSD   +TLLL      GLQ  +  +WF  +AP G
Sbjct: 207 Q-PRGLRMAYYPPCRQADKVMGLSPHSDAGGLTLLLEINNVQGLQIKKDGKWFSIDAPNG 266

Query: 248 SDDALLVSLGDQAEVTSNGKFKSPVHRVLINSERERVSLAVFYLPDSEKEIEPLEELINE 307
              AL+ ++GD  E+ SNGKF+S  HR +IN  +ER+S A+F+ P     I PL E + +
Sbjct: 267 ---ALIANIGDTLEILSNGKFRSVEHRAVINPNKERISAALFHYPSENMVISPLPEFVKD 326

Query: 308 TQPRMYKTVMNLPALYFQYYQQ--GRRTIE 321
            + + Y+++  L  +   + QQ  G+  +E
Sbjct: 327 GKVK-YRSISYLDFMKQIFTQQLDGKNRVE 351

BLAST of Sed0015551 vs. ExPASy Swiss-Prot
Match: D4N501 (Probable 2-oxoglutarate/Fe(II)-dependent dioxygenase OS=Papaver somniferum OX=3469 GN=DIOX2 PE=2 SV=1)

HSP 1 Score: 179.5 bits (454), Expect = 6.1e-44
Identity = 105/323 (32.51%), Postives = 173/323 (53.56%), Query Frame = 0

Query: 1   MASITVADEVPARFIHTEKNRGVLEAPL--------MEAPVIDVSLLSSSS--DSGPELD 60
           +A +T+A E+P+R+I T +N   L+ P+           PVID+  L SS       ELD
Sbjct: 21  LAKLTLA-EIPSRYICTVEN---LQLPVGASVIDDHETVPVIDIENLISSEPVTEKLELD 80

Query: 61  RLRHGLHHWGCFLAIYHGMSTEFLEEVRQVVKQFFALPMDDKQKHLRDENGIEGYGNDMI 120
           RL      WG F  + HG+ T  ++ V+  ++ FF L M++K K+ + +  +EG+G   +
Sbjct: 81  RLHSACKEWGFFQVVNHGVDTSLVDNVKSDIQGFFNLSMNEKIKYGQKDGDVEGFGQAFV 140

Query: 121 LSENQILDWNDRLILTLHPQQSRR----------LKEVVLEYTENVRLISEVILKAMAKS 180
            SE+Q LDW D  ++   P   R+          L+E +  Y+  ++ +S V+ + M K+
Sbjct: 141 ASEDQTLDWADIFMILTLPLHLRKPHLFSKLPLPLRETIESYSSEMKKLSMVLFEKMEKA 200

Query: 181 LDLDENCFLKQYGEQF---TLPARFNFYPKCRNPKLVLGQKPHSDVSAITLLLLDKEAGG 240
           L + +   +K+  E F   T   R N+YP C  P+L +G  PHSD   +T+LL   E  G
Sbjct: 201 LQV-QAVEIKEISEVFKDMTQVMRMNYYPPCPQPELAIGLTPHSDFGGLTILLQLNEVEG 260

Query: 241 LQFLRGNEWFNAPVGSDDALLVSLGDQAEVTSNGKFKSPVHRVLINSERERVSLAVFYLP 300
           LQ      W +      +A +V++GD  E+ +NG ++S  HR ++NS +ER+S+A F+ P
Sbjct: 261 LQIKNEGRWISVK-PLPNAFVVNVGDVLEIMTNGMYRSVDHRAVVNSTKERLSIATFHDP 320

BLAST of Sed0015551 vs. ExPASy Swiss-Prot
Match: Q9FFF6 (Jasmonate-induced oxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=JOX2 PE=1 SV=1)

HSP 1 Score: 176.8 bits (447), Expect = 4.0e-43
Identity = 99/300 (33.00%), Postives = 160/300 (53.33%), Query Frame = 0

Query: 32  PVIDVSLLSSSSDSGPE--LDRLRHGLHHWGCFLAIYHGMSTEFLEEVRQVVKQFFALPM 91
           P+ID+  L S      +  + R+      WG F  + HG+  E ++  R+  ++FF +P+
Sbjct: 63  PIIDLEGLFSEEGLSDDVIMARISEACRGWGFFQVVNHGVKPELMDAARENWREFFHMPV 122

Query: 92  DDKQKHLRDENGIEGYGNDMILSENQILDWNDRLILTLHPQQSR----------RLKEVV 151
           + K+ +       EGYG+ + + +   LDW+D   L L P   +           ++EV+
Sbjct: 123 NAKETYSNSPRTYEGYGSRLGVEKGASLDWSDYYFLHLLPHHLKDFNKWPSFPPTIREVI 182

Query: 152 LEYTENVRLISEVILKAMAKSLDLDENCFLKQY-GEQFTLPARFNFYPKCRNPKLVLGQK 211
            EY E +  +S  I++ ++ +L L E+ F + + GE      R N+YPKC  P+L LG  
Sbjct: 183 DEYGEELVKLSGRIMRVLSTNLGLKEDKFQEAFGGENIGACLRVNYYPKCPRPELALGLS 242

Query: 212 PHSDVSAITLLLLDKEAGGLQFLRGNEWFNAPVGSDDALLVSLGDQAEVTSNGKFKSPVH 271
           PHSD   +T+LL D +  GLQ  + + W         A +V++GDQ ++ SN  +KS  H
Sbjct: 243 PHSDPGGMTILLPDDQVFGLQVRKDDTWITVK-PHPHAFIVNIGDQIQILSNSTYKSVEH 302

Query: 272 RVLINSERERVSLAVFYLPDSEKEIEPLEELINETQPRMYKTVMNLPALYFQYYQQGRRT 319
           RV++NS++ERVSLA FY P S+  I+PL+EL++   P +Y      P + F  Y+   RT
Sbjct: 303 RVIVNSDKERVSLAFFYNPKSDIPIQPLQELVSTHNPPLY------PPMTFDQYRLFIRT 355

BLAST of Sed0015551 vs. ExPASy Swiss-Prot
Match: Q39224 (Protein SRG1 OS=Arabidopsis thaliana OX=3702 GN=SRG1 PE=2 SV=1)

HSP 1 Score: 176.0 bits (445), Expect = 6.8e-43
Identity = 90/305 (29.51%), Postives = 173/305 (56.72%), Query Frame = 0

Query: 10  VPARFIHTEKNRGVLEAPL---MEAPVIDVSLLSSSSDSGPELDRLRHGLHHWGCFLAIY 69
           VP R++ +++++  ++      +E P+ID+  L SS+    E+++L      WG F  + 
Sbjct: 29  VPPRYVRSDQDKTEVDDDFDVKIEIPIIDMKRLCSSTTMDSEVEKLDFACKEWGFFQLVN 88

Query: 70  HGMSTEFLEEVRQVVKQFFALPMDDKQKHLRDENGIEGYGNDMILSENQILDWNDRLILT 129
           HG+ + FL++V+  ++ FF LPM++K+K  +  + IEG+G   ++SE+Q LDW D    T
Sbjct: 89  HGIDSSFLDKVKSEIQDFFNLPMEEKKKFWQRPDEIEGFGQAFVVSEDQKLDWADLFFHT 148

Query: 130 LHPQQSRR----------LKEVVLEYTENVRLISEVILKAMAKSLDLDENCFLKQYGEQF 189
           + P + R+           ++ +  Y+  V+ ++++++  MA++L++      K + +  
Sbjct: 149 VQPVELRKPHLFPKLPLPFRDTLEMYSSEVQSVAKILIAKMARALEIKPEELEKLFDDVD 208

Query: 190 TLPA-RFNFYPKCRNPKLVLGQKPHSDVSAITLLLLDKEAGGLQFLRGNEWFNAPVGS-D 249
           ++ + R N+YP C  P  V+G  PHSD   +T+L+   +  GLQ  +  +W   PV    
Sbjct: 209 SVQSMRMNYYPPCPQPDQVIGLTPHSDSVGLTVLMQVNDVEGLQIKKDGKW--VPVKPLP 268

Query: 250 DALLVSLGDQAEVTSNGKFKSPVHRVLINSERERVSLAVFYLPDSEKEIEPLEELINETQ 300
           +A +V++GD  E+ +NG ++S  HR ++NSE+ER+S+A F+     KE+ P + L+   +
Sbjct: 269 NAFIVNIGDVLEIITNGTYRSIEHRGVVNSEKERLSIATFHNVGMYKEVGPAKSLVERQK 328

BLAST of Sed0015551 vs. ExPASy TrEMBL
Match: A0A5A7U2M0 (Protein SRG1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold675G00660 PE=3 SV=1)

HSP 1 Score: 457.6 bits (1176), Expect = 4.3e-125
Identity = 226/326 (69.33%), Postives = 266/326 (81.60%), Query Frame = 0

Query: 9   EVPARFIHTEKNRGVLEAPLMEAPVIDVSLLSSSSDSGPELDRLRHGLHHWGCFLAIYHG 68
           EVP R+IH E +RG   APLM APVID++LLSSSS SGPEL++LRHGL  WGCF AI HG
Sbjct: 37  EVPERYIHKESDRGARNAPLMAAPVIDIALLSSSSKSGPELEKLRHGLQSWGCFQAINHG 96

Query: 69  MSTEFLEEVRQVVKQFFALPMDDKQKHLRDENGIEGYGNDMILSENQILDWNDRLILTLH 128
           M++E+L+EVRQ+ KQFF L M++K K+L++E+ +EGYGNDMILS  QILDW DRL LT++
Sbjct: 97  MTSEYLDEVRQLTKQFFGLSMEEKLKYLKEEHEMEGYGNDMILSNQQILDWTDRLYLTVY 156

Query: 129 PQQSRRLK----------EVVLEYTENVRLISEVILKAMAKSLDLDENCFLKQYGEQFTL 188
           P QSRR K          EVV EYT NV+LISE ILKAMA+SLDLDE+ FL QYGEQ  L
Sbjct: 157 PHQSRRFKYWPTNPQRFREVVGEYTTNVKLISEKILKAMARSLDLDESSFLNQYGEQVKL 216

Query: 189 PARFNFYPKCRNPKLVLGQKPHSDVSAITLLLLDKEAGGLQFLRGNEWFNAPVGSDDALL 248
            ARFNFYP+CRNP LVLG KPH+D SAIT+LL DKE  GLQF++ NEW+NAP+   DALL
Sbjct: 217 DARFNFYPRCRNPDLVLGVKPHADGSAITILLQDKEVEGLQFMKDNEWYNAPI-VPDALL 276

Query: 249 VSLGDQAEVTSNGKFKSPVHRVLINSERERVSLAVFYLPDSEKEIEPLEELINETQPRMY 308
           V++GDQ E+TSNG FKSPVHRVL NSERER+SLAVFYLPDSEKEIEPLEELINE+QPR+Y
Sbjct: 277 VNVGDQVEITSNGIFKSPVHRVLTNSERERISLAVFYLPDSEKEIEPLEELINESQPRLY 336

Query: 309 KTVMNLPALYFQYYQQGRRTIEAAII 325
           K+V N   LYF+YYQQG+R +EAA I
Sbjct: 337 KSVKNFVGLYFEYYQQGQRPMEAARI 361

BLAST of Sed0015551 vs. ExPASy TrEMBL
Match: A0A5D3CIZ6 (Protein SRG1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold367G00230 PE=3 SV=1)

HSP 1 Score: 457.2 bits (1175), Expect = 5.6e-125
Identity = 226/326 (69.33%), Postives = 266/326 (81.60%), Query Frame = 0

Query: 9   EVPARFIHTEKNRGVLEAPLMEAPVIDVSLLSSSSDSGPELDRLRHGLHHWGCFLAIYHG 68
           EVP R+IH E +RG   APLM APVID++LLSSSS SGPEL++LRHGL  WGCF AI HG
Sbjct: 37  EVPERYIHKESDRGARNAPLMAAPVIDIALLSSSSKSGPELEKLRHGLQSWGCFQAINHG 96

Query: 69  MSTEFLEEVRQVVKQFFALPMDDKQKHLRDENGIEGYGNDMILSENQILDWNDRLILTLH 128
           M++E+L+EVRQ+ KQFF L M++K K+L++E+ +EGYGNDMILS  QILDW DRL LT++
Sbjct: 97  MTSEYLDEVRQLTKQFFGLSMEEKLKYLKEEHEMEGYGNDMILSNQQILDWTDRLYLTVY 156

Query: 129 PQQSRRLK----------EVVLEYTENVRLISEVILKAMAKSLDLDENCFLKQYGEQFTL 188
           P QSRR K          EVV EYT NV+LISE ILKAMA+SLDLDE+ FL QYGEQ  L
Sbjct: 157 PHQSRRFKYWPINPQRFREVVGEYTTNVKLISEKILKAMARSLDLDESSFLNQYGEQVKL 216

Query: 189 PARFNFYPKCRNPKLVLGQKPHSDVSAITLLLLDKEAGGLQFLRGNEWFNAPVGSDDALL 248
            ARFNFYP+CRNP LVLG KPH+D SAIT+LL DKE  GLQF++ NEW+NAP+ + DALL
Sbjct: 217 DARFNFYPRCRNPDLVLGVKPHADGSAITILLQDKEVEGLQFMKDNEWYNAPIVT-DALL 276

Query: 249 VSLGDQAEVTSNGKFKSPVHRVLINSERERVSLAVFYLPDSEKEIEPLEELINETQPRMY 308
           V++GDQ E+TSNG FKSPVHRVL NSERER+SLAVFYLPDSEKEIEPLEELINE+QPR+Y
Sbjct: 277 VNVGDQVEITSNGIFKSPVHRVLTNSERERISLAVFYLPDSEKEIEPLEELINESQPRLY 336

Query: 309 KTVMNLPALYFQYYQQGRRTIEAAII 325
           K+V N   LYF+YYQQG R +EAA I
Sbjct: 337 KSVKNFVGLYFEYYQQGERPMEAARI 361

BLAST of Sed0015551 vs. ExPASy TrEMBL
Match: A0A1S3CP33 (protein SRG1-like OS=Cucumis melo OX=3656 GN=LOC103503160 PE=3 SV=1)

HSP 1 Score: 455.7 bits (1171), Expect = 1.6e-124
Identity = 225/326 (69.02%), Postives = 266/326 (81.60%), Query Frame = 0

Query: 9   EVPARFIHTEKNRGVLEAPLMEAPVIDVSLLSSSSDSGPELDRLRHGLHHWGCFLAIYHG 68
           EVP R+IH E +RG   APLM APVID++LLSSSS SGPEL++LRHGL  WGCF AI HG
Sbjct: 37  EVPERYIHKESDRGARNAPLMAAPVIDIALLSSSSKSGPELEKLRHGLQSWGCFQAINHG 96

Query: 69  MSTEFLEEVRQVVKQFFALPMDDKQKHLRDENGIEGYGNDMILSENQILDWNDRLILTLH 128
           M++E+L+EVRQ+ KQFF L M++K K+L++E+ +EGYGNDMILS  QILDW DRL LT++
Sbjct: 97  MTSEYLDEVRQLTKQFFGLSMEEKLKYLKEEHEMEGYGNDMILSNQQILDWTDRLYLTVY 156

Query: 129 PQQSRRLK----------EVVLEYTENVRLISEVILKAMAKSLDLDENCFLKQYGEQFTL 188
           P QSRR K          EVV EYT NV+LISE ILKAMA+SLDLDE+ FL QYGEQ  L
Sbjct: 157 PHQSRRFKYWPTNPQRFREVVGEYTTNVKLISEKILKAMARSLDLDESSFLSQYGEQVKL 216

Query: 189 PARFNFYPKCRNPKLVLGQKPHSDVSAITLLLLDKEAGGLQFLRGNEWFNAPVGSDDALL 248
            ARFNFYP+CRNP LVLG KPH+D SAIT+LL DK+  GLQF++ NEW+NAP+ + DALL
Sbjct: 217 DARFNFYPRCRNPDLVLGVKPHADGSAITILLQDKKVEGLQFMKDNEWYNAPIVT-DALL 276

Query: 249 VSLGDQAEVTSNGKFKSPVHRVLINSERERVSLAVFYLPDSEKEIEPLEELINETQPRMY 308
           V++GDQ E+TSNG FKSPVHRVL NSERER+SLAVFYLPDSEKEIEPLEELINE+QPR+Y
Sbjct: 277 VNVGDQVEITSNGIFKSPVHRVLTNSERERISLAVFYLPDSEKEIEPLEELINESQPRLY 336

Query: 309 KTVMNLPALYFQYYQQGRRTIEAAII 325
           K+V N   LYF+YYQQG R +EAA I
Sbjct: 337 KSVKNFVGLYFEYYQQGERPMEAARI 361

BLAST of Sed0015551 vs. ExPASy TrEMBL
Match: A0A6J1BYU1 (uncharacterized protein LOC111006992 OS=Momordica charantia OX=3673 GN=LOC111006992 PE=4 SV=1)

HSP 1 Score: 453.4 bits (1165), Expect = 8.1e-124
Identity = 226/326 (69.33%), Postives = 263/326 (80.67%), Query Frame = 0

Query: 9   EVPARFIHTEKNRGVLEAPLMEAPVIDVSLLSSSSDSGPELDRLRHGLHHWGCFLAIYHG 68
           EVP R+IH E +RG L+APLMEAPVID+ LLSS S++GPEL++LRHGLH WGCF AI HG
Sbjct: 18  EVPERYIHKECDRGALDAPLMEAPVIDIGLLSSPSNTGPELEKLRHGLHSWGCFQAINHG 77

Query: 69  MSTEFLEEVRQVVKQFFALPMDDKQKHLRDENGIEGYGNDMILSENQILDWNDRLILTLH 128
           MS EFLEEVRQV K FFALPM+DK KH R+E+ +EGYGNDMI S  QILDW DRL LT+ 
Sbjct: 78  MSPEFLEEVRQVAKLFFALPMEDKLKHSREEDKMEGYGNDMIFSNQQILDWTDRLYLTVC 137

Query: 129 PQQSRRLK----------EVVLEYTENVRLISEVILKAMAKSLDLDENCFLKQYGEQFTL 188
           P++SRR K          EV+ EYT NV+L+SE ILKAMA+SLDLDEN FL QYG++  L
Sbjct: 138 PEESRRFKYWPTNPERFREVLHEYTANVKLLSEKILKAMARSLDLDENSFLNQYGKRVQL 197

Query: 189 PARFNFYPKCRNPKLVLGQKPHSDVSAITLLLLDKEAGGLQFLRGNEWFNAPVGSDDALL 248
            ARFNFY +CRNP LVLG KPH+D SAIT+LL DKE  GLQFL+GNEW+NAP+   DALL
Sbjct: 198 DARFNFYLRCRNPNLVLGVKPHADGSAITILLQDKEVEGLQFLKGNEWWNAPI-IPDALL 257

Query: 249 VSLGDQAEVTSNGKFKSPVHRVLINSERERVSLAVFYLPDSEKEIEPLEELINETQPRMY 308
           V++GDQ E+TSNG FKS VHRVL NSERER+SLAVFYLPD +KEIEPLE+LINET PR+Y
Sbjct: 258 VNVGDQGEITSNGIFKSRVHRVLTNSERERISLAVFYLPDPQKEIEPLEKLINETHPRLY 317

Query: 309 KTVMNLPALYFQYYQQGRRTIEAAII 325
           +TV N   L+FQYYQQG+R  EAA I
Sbjct: 318 RTVKNFVGLFFQYYQQGQRPREAAKI 342

BLAST of Sed0015551 vs. ExPASy TrEMBL
Match: A0A6J1JKD6 (probable 2-oxoglutarate-dependent dioxygenase ANS isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111485215 PE=3 SV=1)

HSP 1 Score: 452.2 bits (1162), Expect = 1.8e-123
Identity = 223/326 (68.40%), Postives = 265/326 (81.29%), Query Frame = 0

Query: 9   EVPARFIHTEKNRGVLEAPLMEAPVIDVSLLSSSSDSGPELDRLRHGLHHWGCFLAIYHG 68
           EVP R+IH E +RG L+APLM APVID++LLSS S SGPEL++LRHGL  WGCF  + HG
Sbjct: 18  EVPERYIHIESDRGALDAPLMAAPVIDMALLSSPSKSGPELEKLRHGLQSWGCFQVVNHG 77

Query: 69  MSTEFLEEVRQVVKQFFALPMDDKQKHLRDENGIEGYGNDMILSENQILDWNDRLILTLH 128
           MS EFL+EVR++ KQFF LPM++K K+ R+EN IEGYGNDMILS  QILDW DRL LT++
Sbjct: 78  MSAEFLDEVRRLAKQFFDLPMEEKSKYSREENEIEGYGNDMILSNQQILDWTDRLYLTVY 137

Query: 129 PQQSRRLK----------EVVLEYTENVRLISEVILKAMAKSLDLDENCFLKQYGEQFTL 188
           P++S R K          EV+ EYT NV+L+SE ILKAMA SLDL+E+ F+KQYGE+  L
Sbjct: 138 PKESHRFKYWPTNPERFREVLHEYTANVKLLSEKILKAMAISLDLNEDSFIKQYGEEVKL 197

Query: 189 PARFNFYPKCRNPKLVLGQKPHSDVSAITLLLLDKEAGGLQFLRGNEWFNAPVGSDDALL 248
            ARFNFYP+CRNP LVLG KPH+D SAIT+LL D+E  GLQFL GNEWFNAP+    ALL
Sbjct: 198 DARFNFYPRCRNPDLVLGVKPHADGSAITILLQDEEVEGLQFLNGNEWFNAPI-VPGALL 257

Query: 249 VSLGDQAEVTSNGKFKSPVHRVLINSERERVSLAVFYLPDSEKEIEPLEELINETQPRMY 308
           V++GDQAE+TSNG FKSPVHRVL NSERER+SLAVFYLPDSEKEIEPLE+LI+ET+PR+Y
Sbjct: 258 VNVGDQAEITSNGIFKSPVHRVLTNSERERISLAVFYLPDSEKEIEPLEKLIDETRPRLY 317

Query: 309 KTVMNLPALYFQYYQQGRRTIEAAII 325
           K+V N   LYFQYYQQG+R +EAA I
Sbjct: 318 KSVKNFVGLYFQYYQQGQRPMEAARI 342

BLAST of Sed0015551 vs. TAIR 10
Match: AT5G20400.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 326.6 bits (836), Expect = 2.2e-89
Identity = 159/337 (47.18%), Postives = 229/337 (67.95%), Query Frame = 0

Query: 4   ITVADEVPARFIHTEKNRGVLE-----APLMEAPVIDVSLLSSSSDSG-PELDRLRHGLH 63
           +   + +P R++H     G ++      P M+ P ID++LL SSS++G  EL +L   L 
Sbjct: 13  VAAGEGLPERYLHAPTGDGEVQPLNAAVPEMDIPAIDLNLLLSSSEAGQQELSKLHSALS 72

Query: 64  HWGCFLAIYHGMSTEFLEEVRQVVKQFFALPMDDKQKHLRDENGIEGYGNDMILSENQIL 123
            WG    + HG++  FL+++ ++ K+FFALP ++KQK  R+ + I+GYGNDMIL ++Q+L
Sbjct: 73  TWGVVQVMNHGITKAFLDKIYKLTKEFFALPTEEKQKCAREIDSIQGYGNDMILWDDQVL 132

Query: 124 DWNDRLILTLHPQQSRRL----------KEVVLEYTENVRLISEVILKAMAKSLDLDENC 183
           DW DRL +T +P+  R+L          +E + EYT   R++ E   KAMA+SL+L+EN 
Sbjct: 133 DWIDRLYITTYPEDQRQLNFWPEVPLGFRETLHEYTMKQRIVIEQFFKAMARSLELEENS 192

Query: 184 FLKQYGEQFTLPARFNFYPKCRNPKLVLGQKPHSDVSAITLLLLDKEAGGLQFLRGNEWF 243
           FL  YGE  TL  RFN YP C +P  V+G KPH+D SAITLLL DK+ GGLQF +  +W+
Sbjct: 193 FLDMYGESATLDTRFNMYPPCPSPDKVIGVKPHADGSAITLLLPDKDVGGLQFQKDGKWY 252

Query: 244 NAPVGSDDALLVSLGDQAEVTSNGKFKSPVHRVLINSERERVSLAVFYLPDSEKEIEPLE 303
            AP+   D +L+++GDQ E+ SNG +KSPVHRV+ N E+ER+S+A F +P ++KEI+P+ 
Sbjct: 253 KAPI-VPDTILINVGDQMEIMSNGIYKSPVHRVVTNREKERISVATFCIPGADKEIQPVN 312

Query: 304 ELINETQPRMYKTVMNLPALYFQYYQQGRRTIEAAII 325
           EL++E +PR+YKTV     LYF+YYQQGRR IEAA+I
Sbjct: 313 ELVSEARPRLYKTVKKYVELYFKYYQQGRRPIEAALI 348

BLAST of Sed0015551 vs. TAIR 10
Match: AT1G49390.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 324.7 bits (831), Expect = 8.4e-89
Identity = 161/337 (47.77%), Postives = 223/337 (66.17%), Query Frame = 0

Query: 4   ITVADEVPARFIHTEKNRGVLE-----APLMEAPVIDVSLL-SSSSDSGPELDRLRHGLH 63
           +     +P R++H     G  +      P M+ P ID+SLL SSS D   E+ +L   L 
Sbjct: 13  VAAGQGLPERYLHAPTGEGESQPLNGAVPEMDIPAIDLSLLFSSSVDGQEEMKKLHSALS 72

Query: 64  HWGCFLAIYHGMSTEFLEEVRQVVKQFFALPMDDKQKHLRDENGIEGYGNDMILSENQIL 123
            WG    + HG++  FL+++ ++ KQFFALP ++K K  R+   I+GYGNDMILS+NQ+L
Sbjct: 73  TWGVVQVMNHGITEAFLDKIYKLTKQFFALPTEEKHKCARETGNIQGYGNDMILSDNQVL 132

Query: 124 DWNDRLILTLHPQQSRRLK----------EVVLEYTENVRLISEVILKAMAKSLDLDENC 183
           DW DRL LT +P+  R+LK          E + EYT   R++ E   KAMA+SL+L+ENC
Sbjct: 133 DWIDRLFLTTYPEDKRQLKFWPQVPVGFSETLDEYTMKQRVLIEKFFKAMARSLELEENC 192

Query: 184 FLKQYGEQFTLPARFNFYPKCRNPKLVLGQKPHSDVSAITLLLLDKEAGGLQFLRGNEWF 243
           FL+ YGE   + +RFNF+P C  P  V+G KPH+D SAITLLL DK+  GLQFL+  +W+
Sbjct: 193 FLEMYGENAVMNSRFNFFPPCPRPDKVIGIKPHADGSAITLLLPDKDVEGLQFLKDGKWY 252

Query: 244 NAPVGSDDALLVSLGDQAEVTSNGKFKSPVHRVLINSERERVSLAVFYLPDSEKEIEPLE 303
            AP+   D +L++LGDQ E+ SNG +KSPVHRV+ N E+ER+S+A F +P  +KEI P +
Sbjct: 253 KAPI-VPDTILITLGDQMEIMSNGIYKSPVHRVVTNREKERISVATFCVPGLDKEIHPAD 312

Query: 304 ELINETQPRMYKTVMNLPALYFQYYQQGRRTIEAAII 325
            L+ E +PR+YKTV     L+++YYQQGRRTIEAA+I
Sbjct: 313 GLVTEARPRLYKTVTKYVDLHYKYYQQGRRTIEAALI 348

BLAST of Sed0015551 vs. TAIR 10
Match: AT5G20550.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 302.8 bits (774), Expect = 3.4e-82
Identity = 151/337 (44.81%), Postives = 219/337 (64.99%), Query Frame = 0

Query: 4   ITVADEVPARFIH---TEKNRGVLEA--PLMEAPVIDVSLLSSSSDSG-PELDRLRHGLH 63
           +   + +P R++     + N   L A  P+M+ P ID+SLL S SD G  EL +L   L 
Sbjct: 13  VAAGEGIPERYLQPPAVDDNGQHLNAAVPVMDIPAIDLSLLLSPSDDGREELSKLHSALS 72

Query: 64  HWGCFLAIYHGMSTEFLEEVRQVVKQFFALPMDDKQKHLRDENGIEGYGNDMILSENQIL 123
            WG    I HG++   L+++ ++ K+F ALP ++KQK+ R+   I+GYGNDMIL ++Q+L
Sbjct: 73  TWGVVQVINHGITKALLDKIYKLTKEFCALPSEEKQKYAREIGSIQGYGNDMILWDDQVL 132

Query: 124 DWNDRLILTLHPQQSRRLK----------EVVLEYTENVRLISEVILKAMAKSLDLDENC 183
           DW DRL +T +P+  R+LK          E + EYT    L+   + KAMA SL+L+ENC
Sbjct: 133 DWIDRLYITTYPEDQRQLKFWPDVPVGFRETLHEYTMKQHLVFNQVFKAMAISLELEENC 192

Query: 184 FLKQYGEQFTLPARFNFYPKCRNPKLVLGQKPHSDVSAITLLLLDKEAGGLQFLRGNEWF 243
           FL   GE  T+  RFN YP C  P  V+G +PH+D SA TLLL DK   GLQFL+  +W+
Sbjct: 193 FLDMCGENATMDTRFNMYPPCPRPDKVIGVRPHADKSAFTLLLPDKNVEGLQFLKDGKWY 252

Query: 244 NAPVGSDDALLVSLGDQAEVTSNGKFKSPVHRVLINSERERVSLAVFYLPDSEKEIEPLE 303
            APV + D +L+++GDQ E+ SNG +KSPVHRV+ N+E+ER+S+A F +P ++KEI+P++
Sbjct: 253 KAPVVASDTILINVGDQMEIMSNGIYKSPVHRVVTNTEKERISVATFCIPGADKEIQPVD 312

Query: 304 ELINETQPRMYKTVMNLPALYFQYYQQGRRTIEAAII 325
            L++E +PR+YK V N   L  +YY QG+R I A++I
Sbjct: 313 GLVSEARPRLYKPVKNYVDLLNKYYIQGQRPIAASLI 349

BLAST of Sed0015551 vs. TAIR 10
Match: AT5G54000.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 299.7 bits (766), Expect = 2.9e-81
Identity = 149/338 (44.08%), Postives = 216/338 (63.91%), Query Frame = 0

Query: 4   ITVADEVPARFIHTEKNRGVLEAPL------MEAPVIDVSLLSSSSDSG-PELDRLRHGL 63
           +   +++P R+++T    G  + P       M+  +ID++LL SSSD G  EL +L   +
Sbjct: 13  VAAGEKLPERYLYTPTGDGEGDQPFNGLLPEMKISIIDLNLLFSSSDDGREELSKLHSAI 72

Query: 64  HHWGCFLAIYHGMSTEFLEEVRQVVKQFFALPMDDKQKHLRDENGIEGYGNDMILSENQI 123
             WG    + HG+S   L+++ ++ KQFF LP  +KQK+ R+ +  +G+GNDMILS++Q+
Sbjct: 73  STWGVVQVMNHGISEALLDKIHELTKQFFVLPTKEKQKYAREISSFQGFGNDMILSDDQV 132

Query: 124 LDWNDRLILTLHPQQSRRLK----------EVVLEYTENVRLISEVILKAMAKSLDLDEN 183
           LDW DRL L  +P+  R+LK          E + EYT   +L+ E   KA+A+SL+L++N
Sbjct: 133 LDWVDRLYLITYPEDQRQLKFWPENPSGFRETLHEYTMKQQLVVEKFFKALARSLELEDN 192

Query: 184 CFLKQYGEQFTLPARFNFYPKCRNPKLVLGQKPHSDVSAITLLLLDKEAGGLQFLRGNEW 243
           CFL+ +GE  TL  RFN YP C  P  VLG KPHSD SA TL+L DK   GLQFL+  +W
Sbjct: 193 CFLEMHGENATLETRFNIYPPCPRPDKVLGLKPHSDGSAFTLILPDKNVEGLQFLKDGKW 252

Query: 244 FNAPVGSDDALLVSLGDQAEVTSNGKFKSPVHRVLINSERERVSLAVFYLPDSEKEIEPL 303
           + A +     +L+++GD  EV SNG +KSPVHRV++N ++ER+ +A F   D +KEI+PL
Sbjct: 253 YKASI-LPHTILINVGDTMEVMSNGIYKSPVHRVVLNGKKERIYVATFCNADEDKEIQPL 312

Query: 304 EELINETQPRMYKTVMNLPALYFQYYQQGRRTIEAAII 325
             L++E +PR+YK V      +F YYQQGRR IEAA+I
Sbjct: 313 NGLVSEARPRLYKAVKKSEKNFFDYYQQGRRPIEAAMI 349

BLAST of Sed0015551 vs. TAIR 10
Match: AT3G21420.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 210.7 bits (535), Expect = 1.8e-54
Identity = 127/340 (37.35%), Postives = 193/340 (56.76%), Query Frame = 0

Query: 8   DEVPARFIHTEKNRGVLEAPLM------EAPVIDVSLLS--SSSDSGPELDRLRHGLHHW 67
           ++VP RFI  E  RGV+ + L       + PVID+S LS   + D   E+ +L      W
Sbjct: 26  NKVPERFIREEYERGVVVSSLKTHHLHHQIPVIDLSKLSKPDNDDFFFEILKLSQACEDW 85

Query: 68  GCFLAIYHGMSTEFLEEVRQVVKQFFALPMDDKQKHLRDENGIEGYGNDMILSENQILDW 127
           G F  I HG+  E +E++ +V  +FF +P+++K+K+  +   ++GYG   I SE+Q LDW
Sbjct: 86  GFFQVINHGIEVEVVEDIEEVASEFFDMPLEEKKKYPMEPGTVQGYGQAFIFSEDQKLDW 145

Query: 128 NDRLILTLHPQQSR----------RLKEVVLEYTENVRLISEVILKAMAKSLDLDENCFL 187
            +   L +HP Q R          R  E +  Y++ +R + + +LK +A SL L E  F 
Sbjct: 146 CNMFALGVHPPQIRNPKLWPSKPARFSESLEGYSKEIRELCKRLLKYIAISLGLKEERFE 205

Query: 188 KQYGEQFTLPARFNFYPKCRNPKLVLGQKPHSDVSAITLLLLDKEA-GGLQFLRGNEWFN 247
           + +GE      R N+YP C +P LVLG  PHSD SA+T+L   K +  GLQ L+ N W  
Sbjct: 206 EMFGEA-VQAVRMNYYPPCSSPDLVLGLSPHSDGSALTVLQQSKNSCVGLQILKDNTW-- 265

Query: 248 APVGS-DDALLVSLGDQAEVTSNGKFKSPVHRVLINSERERVSLAVFYLPDSEKEIEPLE 307
            PV    +AL++++GD  EV SNGK+KS  HR + N E+ER+++  FY P+ E EIEP+ 
Sbjct: 266 VPVKPLPNALVINIGDTIEVLSNGKYKSVEHRAVTNREKERLTIVTFYAPNYEVEIEPMS 325

Query: 308 ELI-NETQPRMYKTVM--NLPALYFQYYQQGRRTIEAAII 325
           EL+ +ET P  Y++    +    Y     QG+++++ A I
Sbjct: 326 ELVDDETNPCKYRSYNHGDYSYHYVSNKLQGKKSLDFAKI 362

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAA0050082.18.9e-12569.33protein SRG1-like [Cucumis melo var. makuwa][more]
TYK10346.11.2e-12469.33protein SRG1-like [Cucumis melo var. makuwa][more]
XP_008465535.13.4e-12469.02PREDICTED: protein SRG1-like [Cucumis melo] >XP_016903407.1 PREDICTED: protein S... [more]
XP_022134811.11.7e-12369.33uncharacterized protein LOC111006992 [Momordica charantia][more]
XP_022987763.13.7e-12368.40probable 2-oxoglutarate-dependent dioxygenase ANS isoform X2 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
O804495.9e-4734.50Jasmonate-induced oxygenase 4 OS=Arabidopsis thaliana OX=3702 GN=JOX4 PE=1 SV=1[more]
Q94LP41.2e-4432.122-oxoglutarate-dependent dioxygenase 11 OS=Oryza sativa subsp. japonica OX=39947... [more]
D4N5016.1e-4432.51Probable 2-oxoglutarate/Fe(II)-dependent dioxygenase OS=Papaver somniferum OX=34... [more]
Q9FFF64.0e-4333.00Jasmonate-induced oxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=JOX2 PE=1 SV=1[more]
Q392246.8e-4329.51Protein SRG1 OS=Arabidopsis thaliana OX=3702 GN=SRG1 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A5A7U2M04.3e-12569.33Protein SRG1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold675G00... [more]
A0A5D3CIZ65.6e-12569.33Protein SRG1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold367G00... [more]
A0A1S3CP331.6e-12469.02protein SRG1-like OS=Cucumis melo OX=3656 GN=LOC103503160 PE=3 SV=1[more]
A0A6J1BYU18.1e-12469.33uncharacterized protein LOC111006992 OS=Momordica charantia OX=3673 GN=LOC111006... [more]
A0A6J1JKD61.8e-12368.40probable 2-oxoglutarate-dependent dioxygenase ANS isoform X2 OS=Cucurbita maxima... [more]
Match NameE-valueIdentityDescription
AT5G20400.12.2e-8947.182-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT1G49390.18.4e-8947.772-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT5G20550.13.4e-8244.812-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT5G54000.12.9e-8144.082-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT3G21420.11.8e-5437.352-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Chayote (edule) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR026992Non-haem dioxygenase N-terminal domainPFAMPF14226DIOX_Ncoord: 32..132
e-value: 1.1E-19
score: 71.3
IPR027443Isopenicillin N synthase-like superfamilyGENE3D2.60.120.330coord: 3..314
e-value: 1.3E-74
score: 253.5
IPR044861Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domainPFAMPF031712OG-FeII_Oxycoord: 182..276
e-value: 1.7E-17
score: 63.7
NoneNo IPR availablePANTHERPTHR47991:SF632-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEINcoord: 5..320
NoneNo IPR availablePANTHERPTHR47991OXOGLUTARATE/IRON-DEPENDENT DIOXYGENASEcoord: 5..320
NoneNo IPR availableSUPERFAMILY51197Clavaminate synthase-likecoord: 8..302
IPR005123Oxoglutarate/iron-dependent dioxygenasePROSITEPS51471FE2OG_OXYcoord: 176..277
score: 11.827957

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sed0015551.1Sed0015551.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0051213 dioxygenase activity
molecular_function GO:0046872 metal ion binding