Sed0012772 (gene) Chayote v1

Overview
NameSed0012772
Typegene
OrganismSechium edule (Chayote v1)
DescriptionLong-chain-alcohol oxidase
LocationLG05: 812552 .. 816137 (+)
RNA-Seq ExpressionSed0012772
SyntenySed0012772
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TCCATTTAATACTAATATCCCAACGGATCAGATGACAGATTCAGCAAAATCCTGAGTTCGTCGGAGTTGAGTCTCAGCCACCGGAGAAGAAATCTGAAGCTCAAAAAAATAGCAAAAATGGCGGATGACGAACAGACTCCTCACCCTCTGTTGAGAAAACGAAGAACAGAGCTCAACCATGGCTTCTCTTCCTCTCAAATGGAAGCCATGGCTGCCTTCTGTGAAGCTCTCATCCCTTCCTTGCCTCTCTCCCAACAAAACCCACTTCACCAATCTCTTCTTTCATTCTACCAATCCTCTGCTTCTCAACCCCCAATTCCAGATGAGGTTATTCATTTTCTCTATTTTGGGCTTAAAATTTGTCGATTCTAAACCAAAAACTTGTTTGTTTGCTTGTTTTTTCATGTGGGTATTTTAGGTTGCTGAGTTAATTGTGGAGAGGGCTCATCCTAAAGCTCTGTTTCTTGTGAAATTGGTTCTGGGAGTTCTCTGTTTCAGACTTGGGACTTTGTTGCTTTGTGGGTGTTTGTGTTTTGATGGGAAATGGCCTTTTGTTCTTAAGTTTTCTGAGATTTCTTTGGAGAATAGGGAAAGGGTTTTGAAGAATTGGTCTGTGGCTCACCATAAATATACTGTGCTTTTGAGAATGGCTTTCATGGTTATTAAAGTTTTCTGCTGCTTTAGATTCTTCTCCAGGGTATGTTTTCTTTGAACACCCTCAGATTTTCCTTGACTTTTGTGTATGTTCTGATTCAAAGTTGCTTAACAATTATCCCTTTTGACTTAATGGTCAATAGTGCATAAGTAATTAAGAGGAGATGAGTTCAATCCATGATAACAACTGATGACAATTGCCTATAAATAATTTCTTACGAATTTCTTTGACATTCAAATATTATAGATTAAAGTATTATGTTATGAAATCAAAACAATAATCAAGATAAAAAAAGAAACAGAGCGTGAGAAATATGGTGATTTATGTGGTTTACGAATTGACTACGTTTATAGATAATGAGATCCGTGATTAAATAATTGTGGGGCATTATACTTCAAGACATACCTCAATATGGTATGTCATGTGAAAATAATCAAGTTGTGCACAAGTTAGCATGGACACTCACAAATTAGAAAAGATAAAAAATCAAAGTTGCTTTAGAAGATTCTGGTTATGATTGATGATAAGATGATATTATTAGGAATATTGTTAGATGCAGTTCATGTTAACAACCTTTCCTGATTTTGTGTAACAACTTATTGTTTAGATCTCATTCATGCCTCTTATAAAAAGGCTCTCTGTATTCTTTATCAATATGCAATACAAGATACAAATTCAAAGTGCAATTTAATTGAAATTCATTTGATATAACCTATGAGCATGAACTGTTACTTTAGTATTCAATGAGTTTTGTTGTGGCATGTGGAAATAGCAACTTGCTTCAATATGAGAGAATATCTTGGTTTTGTTATTAACCAATCGTTCTCCCCCAAATTTTTATCTGTATTGAAATTTATAGCATTACTCTATTTGATCTTTTGTAAGTTAAGGCGTTTAGCTAGTTCACTCAACGAGAAAGATAAATTGGTATTGACCATTAAGAATTATGGGACAAAGGATTATTTTTTGTTTGGTTTCAGGTCGATGAGGAGTCAAAAAATGTAGCATGGGAAGCAATGGGCTACCGAGTAGACACCCAAAAACGGTTGAACAACACCCGAAACGAGCGGCCTCTTGAAAGAGGAGTAGTAGAAACTGGCAAGGAAGATGATTCTACTTTTCTGAAGTCTCTTTCCCTGAAAGGTCTTGAAGCAACTGAAGATCTAAGACATGGTAATGCCTATAAGATCAAATGTGATGTTGCGATTGTTGGCTCGGGTTGCGGTGGCGGAGTTGCTGCTGCGGTTCTTGCAAGATCTGGTTTGAAGGTAGTCGTGCTCGAGAAAGGGAACTATTTTGTGCCTGAAGATTATTCTTCCTTGGAAGGTCCATCCATGAATGAACTATATGAATTAGGTGGACTGAAGTCAACTGTTGATGGGAAGGTTATGCTGTTGGCCGGATCTACTGTGGGTGGAGGCTCTGCTATAAACTGGTCTGCATCGATTAGAACTCCAAAATCTGTTCTTGATGAGTGGTCGACCGATCGAAAGATACCTTTTTTCGGGAGTTTTGAGTACCAATCGGCAATGGATGCAGTGTGCGATAGAATAGGAGTTACCTATGGATGTTCAATAGAAAGCTTCCAAAATCAAGTTTTTCGAAAAGGCTGCGAGAATCTTGGTTTGAAAGTCGATTCGGTTCCGAGGAATTCCTCTGTAAATCATTATTGTGGCTCTTGCTCTTATGGTTGTAGAGTTGGTGACAAGAAAGGGACTGATTCTACTTGGCTGGTTGATGCTGTTGAATCTGGTGCAGTGATCCTCACTGGCTGCAAAGCTGACAAGCTCATTTTGGAGAACACAAATAGCACAAATAGGAACAAGAAATGCTCAGGAGTGGTTGCAACTTTGCTTAGCAAAACCGTCACGAAAAAACTTCATATCGAGGCGAAAGCAACAATCTCGGCCTGTGGTTCGCTCTTGACACCCCCTCTACTGATTGCAAGCGGATTGAATAACAAAAATATAGGCAGGAATCTTCATTTGCACCCTGTTACACTGGCTTGGGGATACTTCCCCGAGCGACTATCCGATTTTACAGGTAAAATACACGAGGGAGGGCTCATTACATCAATCCACAAGGTTGTCTCAGAAGACTCTAATAGCCATGCCATAATCGAAACACCAGCACTTGGACCAGCCTCATTCGCAGCACTGTTTCCATGGACGTCCGGGCGTGACATGAAAGATGCAATGGCAAAGTACGCTCGGACTTCCCACCTTTTCGCTTTGGTAAGAGACCGAGGTGCAGGAGAAGTTAAGGTAAATGCCGATGTCAAGTATCGACTCGACCAAACAGATAAAGAGAATCTAAAAATCGGTTTGAGGCAGGCATTGAGAATCTTGATTGCAGCAGGAGCAACTGAGGTGGGCACGTATAGAAGTGATGGGCAGAGAATCAGGTGTGAAGGGGCAAACAAGGAAGACATCGAGGAGTTTCTCGACACGGTCGAGGCAGTCGGGGGACCGTCGTCGAGGGAAGAGCACTGGACGATGTACTGCTCGGCACATCAGATGAGCAGCTGCAGAATGGGCAGCACAGAGGAAGAAGGAGCAGTGGATGAGAATGGTGAAAGTTGGGAAGCAGAAGGGTTGTTTGTATGTGATGGAAGTGTGCTGCCAACTGCAGTTGGAGTCAATCCAATGATCACAATTCAATCCACTGCTTATTGCATCTCAAACAGGATTGCACACTCTTTCAATAAATAAGAAACTTCATGAAAGATCTTTAAATGTTCAATTAGCTCTTGGGAAAGCTTTAGTTTTTTTAAATATTACTATTTACTTTTGTTAACTTATTGTATCAATGTTGATTTTTTTTTTTATATTATATGTGGTCAAGTTTATTGGTTTTGGAAGTGGGAGATTTGAATATGTACCTCTCTTATTATTAAAGATCTATAAAATACAACTAGAGTTATGATGGTTC

mRNA sequence

TCCATTTAATACTAATATCCCAACGGATCAGATGACAGATTCAGCAAAATCCTGAGTTCGTCGGAGTTGAGTCTCAGCCACCGGAGAAGAAATCTGAAGCTCAAAAAAATAGCAAAAATGGCGGATGACGAACAGACTCCTCACCCTCTGTTGAGAAAACGAAGAACAGAGCTCAACCATGGCTTCTCTTCCTCTCAAATGGAAGCCATGGCTGCCTTCTGTGAAGCTCTCATCCCTTCCTTGCCTCTCTCCCAACAAAACCCACTTCACCAATCTCTTCTTTCATTCTACCAATCCTCTGCTTCTCAACCCCCAATTCCAGATGAGGTTATTCATTTTCTCTATTTTGGGCTTAAAATTTGTCGATTCTAAACCAAAAACTTGTTTGTTTGCTTGTTTTTTCATGTGGGTATTTTAGGTTGCTGAGTTAATTGTGGAGAGGGCTCATCCTAAAGCTCTGTTTCTTGTGAAATTGGTTCTGGGAGTTCTCTGTTTCAGACTTGGGACTTTGTTGCTTTGTGGGTGTTTGTGTTTTGATGGGAAATGGCCTTTTGTTCTTAAGTTTTCTGAGATTTCTTTGGAGAATAGGGAAAGGGTTTTGAAGAATTGGTCTGTGGCTCACCATAAATATACTGTGCTTTTGAGAATGGCTTTCATGGTTATTAAAGTTTTCTGCTGCTTTAGATTCTTCTCCAGGGTCGATGAGGAGTCAAAAAATGTAGCATGGGAAGCAATGGGCTACCGAGTAGACACCCAAAAACGGTTGAACAACACCCGAAACGAGCGGCCTCTTGAAAGAGGAGTAGTAGAAACTGGCAAGGAAGATGATTCTACTTTTCTGAAGTCTCTTTCCCTGAAAGGTCTTGAAGCAACTGAAGATCTAAGACATGGTAATGCCTATAAGATCAAATGTGATGTTGCGATTGTTGGCTCGGGTTGCGGTGGCGGAGTTGCTGCTGCGGTTCTTGCAAGATCTGGTTTGAAGGTAGTCGTGCTCGAGAAAGGGAACTATTTTGTGCCTGAAGATTATTCTTCCTTGGAAGGTCCATCCATGAATGAACTATATGAATTAGGTGGACTGAAGTCAACTGTTGATGGGAAGGTTATGCTGTTGGCCGGATCTACTGTGGGTGGAGGCTCTGCTATAAACTGGTCTGCATCGATTAGAACTCCAAAATCTGTTCTTGATGAGTGGTCGACCGATCGAAAGATACCTTTTTTCGGGAGTTTTGAGTACCAATCGGCAATGGATGCAGTGTGCGATAGAATAGGAGTTACCTATGGATGTTCAATAGAAAGCTTCCAAAATCAAGTTTTTCGAAAAGGCTGCGAGAATCTTGGTTTGAAAGTCGATTCGGTTCCGAGGAATTCCTCTGTAAATCATTATTGTGGCTCTTGCTCTTATGGTTGTAGAGTTGGTGACAAGAAAGGGACTGATTCTACTTGGCTGGTTGATGCTGTTGAATCTGGTGCAGTGATCCTCACTGGCTGCAAAGCTGACAAGCTCATTTTGGAGAACACAAATAGCACAAATAGGAACAAGAAATGCTCAGGAGTGGTTGCAACTTTGCTTAGCAAAACCGTCACGAAAAAACTTCATATCGAGGCGAAAGCAACAATCTCGGCCTGTGGTTCGCTCTTGACACCCCCTCTACTGATTGCAAGCGGATTGAATAACAAAAATATAGGCAGGAATCTTCATTTGCACCCTGTTACACTGGCTTGGGGATACTTCCCCGAGCGACTATCCGATTTTACAGGTAAAATACACGAGGGAGGGCTCATTACATCAATCCACAAGGTTGTCTCAGAAGACTCTAATAGCCATGCCATAATCGAAACACCAGCACTTGGACCAGCCTCATTCGCAGCACTGTTTCCATGGACGTCCGGGCGTGACATGAAAGATGCAATGGCAAAGTACGCTCGGACTTCCCACCTTTTCGCTTTGGTAAGAGACCGAGGTGCAGGAGAAGTTAAGGTAAATGCCGATGTCAAGTATCGACTCGACCAAACAGATAAAGAGAATCTAAAAATCGGTTTGAGGCAGGCATTGAGAATCTTGATTGCAGCAGGAGCAACTGAGGTGGGCACGTATAGAAGTGATGGGCAGAGAATCAGGTGTGAAGGGGCAAACAAGGAAGACATCGAGGAGTTTCTCGACACGGTCGAGGCAGTCGGGGGACCGTCGTCGAGGGAAGAGCACTGGACGATGTACTGCTCGGCACATCAGATGAGCAGCTGCAGAATGGGCAGCACAGAGGAAGAAGGAGCAGTGGATGAGAATGGTGAAAGTTGGGAAGCAGAAGGGTTGTTTGTATGTGATGGAAGTGTGCTGCCAACTGCAGTTGGAGTCAATCCAATGATCACAATTCAATCCACTGCTTATTGCATCTCAAACAGGATTGCACACTCTTTCAATAAATAAGAAACTTCATGAAAGATCTTTAAATGTTCAATTAGCTCTTGGGAAAGCTTTAGTTTTTTTAAATATTACTATTTACTTTTGTTAACTTATTGTATCAATGTTGATTTTTTTTTTTATATTATATGTGGTCAAGTTTATTGGTTTTGGAAGTGGGAGATTTGAATATGTACCTCTCTTATTATTAAAGATCTATAAAATACAACTAGAGTTATGATGGTTC

Coding sequence (CDS)

ATGGCTTTCATGGTTATTAAAGTTTTCTGCTGCTTTAGATTCTTCTCCAGGGTCGATGAGGAGTCAAAAAATGTAGCATGGGAAGCAATGGGCTACCGAGTAGACACCCAAAAACGGTTGAACAACACCCGAAACGAGCGGCCTCTTGAAAGAGGAGTAGTAGAAACTGGCAAGGAAGATGATTCTACTTTTCTGAAGTCTCTTTCCCTGAAAGGTCTTGAAGCAACTGAAGATCTAAGACATGGTAATGCCTATAAGATCAAATGTGATGTTGCGATTGTTGGCTCGGGTTGCGGTGGCGGAGTTGCTGCTGCGGTTCTTGCAAGATCTGGTTTGAAGGTAGTCGTGCTCGAGAAAGGGAACTATTTTGTGCCTGAAGATTATTCTTCCTTGGAAGGTCCATCCATGAATGAACTATATGAATTAGGTGGACTGAAGTCAACTGTTGATGGGAAGGTTATGCTGTTGGCCGGATCTACTGTGGGTGGAGGCTCTGCTATAAACTGGTCTGCATCGATTAGAACTCCAAAATCTGTTCTTGATGAGTGGTCGACCGATCGAAAGATACCTTTTTTCGGGAGTTTTGAGTACCAATCGGCAATGGATGCAGTGTGCGATAGAATAGGAGTTACCTATGGATGTTCAATAGAAAGCTTCCAAAATCAAGTTTTTCGAAAAGGCTGCGAGAATCTTGGTTTGAAAGTCGATTCGGTTCCGAGGAATTCCTCTGTAAATCATTATTGTGGCTCTTGCTCTTATGGTTGTAGAGTTGGTGACAAGAAAGGGACTGATTCTACTTGGCTGGTTGATGCTGTTGAATCTGGTGCAGTGATCCTCACTGGCTGCAAAGCTGACAAGCTCATTTTGGAGAACACAAATAGCACAAATAGGAACAAGAAATGCTCAGGAGTGGTTGCAACTTTGCTTAGCAAAACCGTCACGAAAAAACTTCATATCGAGGCGAAAGCAACAATCTCGGCCTGTGGTTCGCTCTTGACACCCCCTCTACTGATTGCAAGCGGATTGAATAACAAAAATATAGGCAGGAATCTTCATTTGCACCCTGTTACACTGGCTTGGGGATACTTCCCCGAGCGACTATCCGATTTTACAGGTAAAATACACGAGGGAGGGCTCATTACATCAATCCACAAGGTTGTCTCAGAAGACTCTAATAGCCATGCCATAATCGAAACACCAGCACTTGGACCAGCCTCATTCGCAGCACTGTTTCCATGGACGTCCGGGCGTGACATGAAAGATGCAATGGCAAAGTACGCTCGGACTTCCCACCTTTTCGCTTTGGTAAGAGACCGAGGTGCAGGAGAAGTTAAGGTAAATGCCGATGTCAAGTATCGACTCGACCAAACAGATAAAGAGAATCTAAAAATCGGTTTGAGGCAGGCATTGAGAATCTTGATTGCAGCAGGAGCAACTGAGGTGGGCACGTATAGAAGTGATGGGCAGAGAATCAGGTGTGAAGGGGCAAACAAGGAAGACATCGAGGAGTTTCTCGACACGGTCGAGGCAGTCGGGGGACCGTCGTCGAGGGAAGAGCACTGGACGATGTACTGCTCGGCACATCAGATGAGCAGCTGCAGAATGGGCAGCACAGAGGAAGAAGGAGCAGTGGATGAGAATGGTGAAAGTTGGGAAGCAGAAGGGTTGTTTGTATGTGATGGAAGTGTGCTGCCAACTGCAGTTGGAGTCAATCCAATGATCACAATTCAATCCACTGCTTATTGCATCTCAAACAGGATTGCACACTCTTTCAATAAATAA

Protein sequence

MAFMVIKVFCCFRFFSRVDEESKNVAWEAMGYRVDTQKRLNNTRNERPLERGVVETGKEDDSTFLKSLSLKGLEATEDLRHGNAYKIKCDVAIVGSGCGGGVAAAVLARSGLKVVVLEKGNYFVPEDYSSLEGPSMNELYELGGLKSTVDGKVMLLAGSTVGGGSAINWSASIRTPKSVLDEWSTDRKIPFFGSFEYQSAMDAVCDRIGVTYGCSIESFQNQVFRKGCENLGLKVDSVPRNSSVNHYCGSCSYGCRVGDKKGTDSTWLVDAVESGAVILTGCKADKLILENTNSTNRNKKCSGVVATLLSKTVTKKLHIEAKATISACGSLLTPPLLIASGLNNKNIGRNLHLHPVTLAWGYFPERLSDFTGKIHEGGLITSIHKVVSEDSNSHAIIETPALGPASFAALFPWTSGRDMKDAMAKYARTSHLFALVRDRGAGEVKVNADVKYRLDQTDKENLKIGLRQALRILIAAGATEVGTYRSDGQRIRCEGANKEDIEEFLDTVEAVGGPSSREEHWTMYCSAHQMSSCRMGSTEEEGAVDENGESWEAEGLFVCDGSVLPTAVGVNPMITIQSTAYCISNRIAHSFNK
Homology
BLAST of Sed0012772 vs. NCBI nr
Match: XP_038888257.1 (long-chain-alcohol oxidase FAO2-like [Benincasa hispida])

HSP 1 Score: 1045.8 bits (2703), Expect = 1.4e-301
Identity = 508/592 (85.81%), Postives = 552/592 (93.24%), Query Frame = 0

Query: 1   MAFMVIKVFCCFRFFSRVDEESKNVAWEAMGYRVDTQKRLNNTRNERPLERGVVETGKED 60
           M FM+IK+FCCF+FFSR+D E+KNVAW+AMGY VDT+KRLN+TR ERPLERG++ETG ED
Sbjct: 147 MTFMIIKIFCCFKFFSRIDGETKNVAWKAMGYHVDTRKRLNDTRKERPLERGMIETGNED 206

Query: 61  DSTFLKSLSLKGLEATEDLRHGNAYKIKCDVAIVGSGCGGGVAAAVLARSGLKVVVLEKG 120
           DSTF KSL+LKGLE TED +HGNAYKIKCDV IVGSGCGGGVAAAVLA+SGLKVVVLEKG
Sbjct: 207 DSTFPKSLTLKGLEVTEDKKHGNAYKIKCDVVIVGSGCGGGVAAAVLAKSGLKVVVLEKG 266

Query: 121 NYFVPEDYSSLEGPSMNELYELGGLKSTVDGKVMLLAGSTVGGGSAINWSASIRTPKSVL 180
           NYFV EDYSSLEGPSMNELYE GGLKST+DGKVMLLAG+TVGGGSAINWSASIRTPKSVL
Sbjct: 267 NYFVAEDYSSLEGPSMNELYESGGLKSTLDGKVMLLAGTTVGGGSAINWSASIRTPKSVL 326

Query: 181 DEWSTDRKIPFFGSFEYQSAMDAVCDRIGVTYGCSIESFQNQVFRKGCENLGLKVDSVPR 240
           DEWSTD KIP F S +YQ AMDAVCDRIGV  GCS+ESFQNQV RKGCENLGLKVD V R
Sbjct: 327 DEWSTDHKIPLFRSIDYQLAMDAVCDRIGVIEGCSLESFQNQVLRKGCENLGLKVDLVSR 386

Query: 241 NSSVNHYCGSCSYGCRVGDKKGTDSTWLVDAVESGAVILTGCKADKLILENTNSTNRNKK 300
           NSS +HYCGSC YGCRVGDKKGTDSTWLVDAVESGAVILTGC+ADK ILENTN+T+RNK+
Sbjct: 387 NSSKDHYCGSCCYGCRVGDKKGTDSTWLVDAVESGAVILTGCQADKFILENTNNTSRNKR 446

Query: 301 CSGVVATLLSKTVTKKLHIEAKATISACGSLLTPPLLIASGLNNKNIGRNLHLHPVTLAW 360
           C GV+AT LSK +TKKLHIEAKATISACGSLLTPPLLIASGL NKNIG NLHLHPV LAW
Sbjct: 447 CLGVIATPLSKNITKKLHIEAKATISACGSLLTPPLLIASGLRNKNIGSNLHLHPVILAW 506

Query: 361 GYFPERLSDFTGKIHEGGLITSIHKVVSEDSNSHAIIETPALGPASFAALFPWTSGRDMK 420
           GYFPE LSDFTGKI+EGGLITS+HKVVS+DS+SHAIIETPALG ASFAALFPW SGR+MK
Sbjct: 507 GYFPEELSDFTGKIYEGGLITSLHKVVSKDSHSHAIIETPALGSASFAALFPWISGREMK 566

Query: 421 DAMAKYARTSHLFALVRDRGAGEVKVNADVKYRLDQTDKENLKIGLRQALRILIAAGATE 480
           DAM KYART+HLFALVRD+GAGE+KV A+VKYRL+QTDKENL+IGLRQALRILIAAGATE
Sbjct: 567 DAMVKYARTAHLFALVRDQGAGEIKVKANVKYRLNQTDKENLRIGLRQALRILIAAGATE 626

Query: 481 VGTYRSDGQRIRCEGANKEDIEEFLDTVEAVGGPSSREEHWTMYCSAHQMSSCRMGSTEE 540
           VGTYRSDGQRI+C+G NKED+EEFLD+VEAVGGP SRE+HWTMYCSAHQMSSC+MGST+E
Sbjct: 627 VGTYRSDGQRIKCQGTNKEDLEEFLDSVEAVGGPLSREKHWTMYCSAHQMSSCKMGSTKE 686

Query: 541 EGAVDENGESWEAEGLFVCDGSVLPTAVGVNPMITIQSTAYCISNRIAHSFN 593
           EGAVDENGESWEAEGLFVCDGSVLPTA+GVNPMITIQSTAYCIS +IA S N
Sbjct: 687 EGAVDENGESWEAEGLFVCDGSVLPTAIGVNPMITIQSTAYCISKKIAESLN 738

BLAST of Sed0012772 vs. NCBI nr
Match: XP_022967423.1 (long-chain-alcohol oxidase FAO2-like [Cucurbita maxima] >XP_022967424.1 long-chain-alcohol oxidase FAO2-like [Cucurbita maxima])

HSP 1 Score: 1044.3 bits (2699), Expect = 4.1e-301
Identity = 504/592 (85.14%), Postives = 551/592 (93.07%), Query Frame = 0

Query: 1   MAFMVIKVFCCFRFFSRVDEESKNVAWEAMGYRVDTQKRLNNTRNERPLERGVVETGKED 60
           M FM+IK+FCCF+FFSR+D ESKN+AWEAMGY VDT+KRLN++R ERPLERG++ETGKED
Sbjct: 147 MVFMIIKMFCCFKFFSRIDRESKNIAWEAMGYHVDTRKRLNDSRKERPLERGLIETGKED 206

Query: 61  DSTFLKSLSLKGLEATEDLRHGNAYKIKCDVAIVGSGCGGGVAAAVLARSGLKVVVLEKG 120
           DST L+SLS+KGL  TED +HG+ Y IKCDV IVGSGCGGGVAAAVLA+SGLKVVVLEKG
Sbjct: 207 DSTLLRSLSMKGLAVTEDQKHGSTYTIKCDVVIVGSGCGGGVAAAVLAKSGLKVVVLEKG 266

Query: 121 NYFVPEDYSSLEGPSMNELYELGGLKSTVDGKVMLLAGSTVGGGSAINWSASIRTPKSVL 180
           NYFVPEDYSSLEGPSMNELYE GGLKST+DGKVMLLAG+TVGGGSAINWSASIRTPK VL
Sbjct: 267 NYFVPEDYSSLEGPSMNELYETGGLKSTLDGKVMLLAGTTVGGGSAINWSASIRTPKPVL 326

Query: 181 DEWSTDRKIPFFGSFEYQSAMDAVCDRIGVTYGCSIESFQNQVFRKGCENLGLKVDSVPR 240
           +EWST  KIP FGSF+YQSAMDAVCDRIGVT GC +ESFQNQV RKGCENLGLKVDSVPR
Sbjct: 327 EEWSTIHKIPLFGSFDYQSAMDAVCDRIGVTEGCRLESFQNQVLRKGCENLGLKVDSVPR 386

Query: 241 NSSVNHYCGSCSYGCRVGDKKGTDSTWLVDAVESGAVILTGCKADKLILENTNSTNRNKK 300
           NSS +HYCGSC YGCRVGDKKGTDSTWLVDAVESGAVILTGC+ADKL+LENTN+T+RNK+
Sbjct: 387 NSSKDHYCGSCCYGCRVGDKKGTDSTWLVDAVESGAVILTGCRADKLVLENTNNTSRNKR 446

Query: 301 CSGVVATLLSKTVTKKLHIEAKATISACGSLLTPPLLIASGLNNKNIGRNLHLHPVTLAW 360
           C GV+AT LSK VTKKLHIEAKATISACGSLLTPPLLIASGL NKNIG NLHLHPV LAW
Sbjct: 447 CLGVIATPLSKNVTKKLHIEAKATISACGSLLTPPLLIASGLRNKNIGSNLHLHPVILAW 506

Query: 361 GYFPERLSDFTGKIHEGGLITSIHKVVSEDSNSHAIIETPALGPASFAALFPWTSGRDMK 420
           GYFPE+LSDFTGKI+EGGLITS+H VVS+DSNSHAII+TPALGPASFAA+FPWTSGRDMK
Sbjct: 507 GYFPEQLSDFTGKIYEGGLITSLHNVVSKDSNSHAIIQTPALGPASFAAMFPWTSGRDMK 566

Query: 421 DAMAKYARTSHLFALVRDRGAGEVKVNADVKYRLDQTDKENLKIGLRQALRILIAAGATE 480
           DA+ KYART+HLFALVRDRGAGE+KV A+VKYRLDQTDKENL+IGLRQALRILIAAGATE
Sbjct: 567 DAVVKYARTAHLFALVRDRGAGEIKVKANVKYRLDQTDKENLRIGLRQALRILIAAGATE 626

Query: 481 VGTYRSDGQRIRCEGANKEDIEEFLDTVEAVGGPSSREEHWTMYCSAHQMSSCRMGSTEE 540
           VGTYRSDGQR +  G NKED+EEF D+VEA GGP SRE++W +YCSAHQMSSCRMGSTEE
Sbjct: 627 VGTYRSDGQRFQFHGTNKEDLEEFFDSVEAAGGPFSREKYWNIYCSAHQMSSCRMGSTEE 686

Query: 541 EGAVDENGESWEAEGLFVCDGSVLPTAVGVNPMITIQSTAYCISNRIAHSFN 593
           EGAVDENGESWEA+GLFVCDGSVLPTAVGVNPMITIQSTAYCIS ++A S N
Sbjct: 687 EGAVDENGESWEAKGLFVCDGSVLPTAVGVNPMITIQSTAYCISKKLAESLN 738

BLAST of Sed0012772 vs. NCBI nr
Match: XP_022963812.1 (long-chain-alcohol oxidase FAO2 [Cucurbita moschata] >XP_022963813.1 long-chain-alcohol oxidase FAO2 [Cucurbita moschata])

HSP 1 Score: 1041.2 bits (2691), Expect = 3.5e-300
Identity = 502/592 (84.80%), Postives = 551/592 (93.07%), Query Frame = 0

Query: 1   MAFMVIKVFCCFRFFSRVDEESKNVAWEAMGYRVDTQKRLNNTRNERPLERGVVETGKED 60
           M FM+IK+FCCF+FFS +D ESKN+AWEAMGYRVDT+KRLN+TR ERPLERGV+E GKED
Sbjct: 147 MVFMIIKMFCCFKFFSGIDRESKNIAWEAMGYRVDTRKRLNDTRKERPLERGVIEAGKED 206

Query: 61  DSTFLKSLSLKGLEATEDLRHGNAYKIKCDVAIVGSGCGGGVAAAVLARSGLKVVVLEKG 120
           DST L+SLS+KGL  TED +HG+ Y IKCDV IVGSGCGGGVAAAVLA+SGLKVVVLEKG
Sbjct: 207 DSTLLQSLSMKGLAVTEDPKHGSTYTIKCDVVIVGSGCGGGVAAAVLAKSGLKVVVLEKG 266

Query: 121 NYFVPEDYSSLEGPSMNELYELGGLKSTVDGKVMLLAGSTVGGGSAINWSASIRTPKSVL 180
           NYFVPEDYSSLEGPSMNELYE GGLKST+DGKVMLLAG+TVGGGSAINWSASIRTPK VL
Sbjct: 267 NYFVPEDYSSLEGPSMNELYETGGLKSTIDGKVMLLAGTTVGGGSAINWSASIRTPKPVL 326

Query: 181 DEWSTDRKIPFFGSFEYQSAMDAVCDRIGVTYGCSIESFQNQVFRKGCENLGLKVDSVPR 240
           +EWST  KIP FGSF+Y+SAMDAVCDRIGVT GC +ESFQNQV RKGCENLGLKVDSVPR
Sbjct: 327 EEWSTIHKIPLFGSFDYRSAMDAVCDRIGVTEGCRLESFQNQVLRKGCENLGLKVDSVPR 386

Query: 241 NSSVNHYCGSCSYGCRVGDKKGTDSTWLVDAVESGAVILTGCKADKLILENTNSTNRNKK 300
           NSS +HYCGSC YGCRVGDKKGTD+TWLVDAVESGAVILTGC+ADKL+LENTN+T+RNK+
Sbjct: 387 NSSKDHYCGSCCYGCRVGDKKGTDATWLVDAVESGAVILTGCRADKLVLENTNNTSRNKR 446

Query: 301 CSGVVATLLSKTVTKKLHIEAKATISACGSLLTPPLLIASGLNNKNIGRNLHLHPVTLAW 360
           C GV+AT+LSK VTKKL+IEAKATISACGSLLTPPLLIASGL NKNIG NLHLHPV LAW
Sbjct: 447 CLGVIATMLSKNVTKKLYIEAKATISACGSLLTPPLLIASGLRNKNIGSNLHLHPVILAW 506

Query: 361 GYFPERLSDFTGKIHEGGLITSIHKVVSEDSNSHAIIETPALGPASFAALFPWTSGRDMK 420
           GYFPE+LSDFTGKI+EGGLITS+H VVS+DSNSHAII+TPALGPASFAA+FPWTSGRDMK
Sbjct: 507 GYFPEQLSDFTGKIYEGGLITSLHNVVSKDSNSHAIIQTPALGPASFAAMFPWTSGRDMK 566

Query: 421 DAMAKYARTSHLFALVRDRGAGEVKVNADVKYRLDQTDKENLKIGLRQALRILIAAGATE 480
           DAM KYART+HLFALVRD+GAGE+K  A+VKYRLDQTDKENL+IGLRQALRILIAAGATE
Sbjct: 567 DAMVKYARTAHLFALVRDKGAGEIKAKANVKYRLDQTDKENLRIGLRQALRILIAAGATE 626

Query: 481 VGTYRSDGQRIRCEGANKEDIEEFLDTVEAVGGPSSREEHWTMYCSAHQMSSCRMGSTEE 540
           VGTYRS+GQR R  G NKED+EEFLD+VEA GGP SRE++W +YCSAHQMSSCRMGSTEE
Sbjct: 627 VGTYRSNGQRFRFHGTNKEDLEEFLDSVEAAGGPFSREKYWNIYCSAHQMSSCRMGSTEE 686

Query: 541 EGAVDENGESWEAEGLFVCDGSVLPTAVGVNPMITIQSTAYCISNRIAHSFN 593
           EGAVDENGESWEA+GLFVCDGSVLPTAVGVNPMITIQSTAYCIS ++A S N
Sbjct: 687 EGAVDENGESWEAKGLFVCDGSVLPTAVGVNPMITIQSTAYCISKKLAESLN 738

BLAST of Sed0012772 vs. NCBI nr
Match: KAG7011279.1 (Long-chain-alcohol oxidase FAO2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1041.2 bits (2691), Expect = 3.5e-300
Identity = 501/590 (84.92%), Postives = 550/590 (93.22%), Query Frame = 0

Query: 1   MAFMVIKVFCCFRFFSRVDEESKNVAWEAMGYRVDTQKRLNNTRNERPLERGVVETGKED 60
           M FM+IK+FCCF+FFSR+D ESKN+AWEAMGYRVDT+KRLN+TR ERPLERG++E GKED
Sbjct: 150 MVFMIIKMFCCFKFFSRIDGESKNIAWEAMGYRVDTRKRLNDTRKERPLERGLIEAGKED 209

Query: 61  DSTFLKSLSLKGLEATEDLRHGNAYKIKCDVAIVGSGCGGGVAAAVLARSGLKVVVLEKG 120
           DST L+SLS+KGL  TED +HG+ Y IKCDV I+GSGCGGGVAAA+LA+SGLKVVVLEKG
Sbjct: 210 DSTLLQSLSMKGLAVTEDQKHGSTYTIKCDVVIIGSGCGGGVAAAILAKSGLKVVVLEKG 269

Query: 121 NYFVPEDYSSLEGPSMNELYELGGLKSTVDGKVMLLAGSTVGGGSAINWSASIRTPKSVL 180
           NYFVPEDYSSLEGPSMNELYE GGLKST+DGKVMLLAG+TVGGGSAINWSASIRTPK VL
Sbjct: 270 NYFVPEDYSSLEGPSMNELYETGGLKSTIDGKVMLLAGTTVGGGSAINWSASIRTPKPVL 329

Query: 181 DEWSTDRKIPFFGSFEYQSAMDAVCDRIGVTYGCSIESFQNQVFRKGCENLGLKVDSVPR 240
           +EWST  KIP FGSF+Y+SAMDAVCDRIGVT GC +ESFQNQV RKGCENLGLKVDSVPR
Sbjct: 330 EEWSTIHKIPLFGSFDYRSAMDAVCDRIGVTEGCRLESFQNQVLRKGCENLGLKVDSVPR 389

Query: 241 NSSVNHYCGSCSYGCRVGDKKGTDSTWLVDAVESGAVILTGCKADKLILENTNSTNRNKK 300
           NSS +HYCGSC YGCRVGDKKGTDSTWLVDAVESGAVILTGC+ADKL+LENTN+T+RNK+
Sbjct: 390 NSSKDHYCGSCCYGCRVGDKKGTDSTWLVDAVESGAVILTGCRADKLVLENTNNTSRNKR 449

Query: 301 CSGVVATLLSKTVTKKLHIEAKATISACGSLLTPPLLIASGLNNKNIGRNLHLHPVTLAW 360
           C GV+AT LSK VTKKL+IEAKATISACGSLLTPPLLIASGL NKNIG NLHLHPV LAW
Sbjct: 450 CLGVIATTLSKNVTKKLYIEAKATISACGSLLTPPLLIASGLRNKNIGSNLHLHPVILAW 509

Query: 361 GYFPERLSDFTGKIHEGGLITSIHKVVSEDSNSHAIIETPALGPASFAALFPWTSGRDMK 420
           GYFPE+LSDFTGKI+EGGLITS+H VVS+DSNSHAII+TPALGPASFAA+FPWTSGRDMK
Sbjct: 510 GYFPEQLSDFTGKIYEGGLITSLHNVVSKDSNSHAIIQTPALGPASFAAMFPWTSGRDMK 569

Query: 421 DAMAKYARTSHLFALVRDRGAGEVKVNADVKYRLDQTDKENLKIGLRQALRILIAAGATE 480
           DAM KYART+HLFALVRD+GAGE+K  A+VKYRLDQTDKENL+IGLRQALRILIAAGATE
Sbjct: 570 DAMVKYARTAHLFALVRDKGAGEIKAKANVKYRLDQTDKENLRIGLRQALRILIAAGATE 629

Query: 481 VGTYRSDGQRIRCEGANKEDIEEFLDTVEAVGGPSSREEHWTMYCSAHQMSSCRMGSTEE 540
           VGTYRSDGQR R  G NKED+EEFLD+VEA GGP SRE++W +YCSAHQMSSCRMGSTEE
Sbjct: 630 VGTYRSDGQRFRFHGTNKEDLEEFLDSVEAAGGPFSREKYWNIYCSAHQMSSCRMGSTEE 689

Query: 541 EGAVDENGESWEAEGLFVCDGSVLPTAVGVNPMITIQSTAYCISNRIAHS 591
           EGAVDENGESWEA+GLFVCDGSVLPTAVGVNPMITIQSTAYCIS ++A S
Sbjct: 690 EGAVDENGESWEAKGLFVCDGSVLPTAVGVNPMITIQSTAYCISKKLAES 739

BLAST of Sed0012772 vs. NCBI nr
Match: XP_023553773.1 (long-chain-alcohol oxidase FAO2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1033.5 bits (2671), Expect = 7.2e-298
Identity = 499/588 (84.86%), Postives = 548/588 (93.20%), Query Frame = 0

Query: 1   MAFMVIKVFCCFRFFSRVDEESKNVAWEAMGYRVDTQKRLNNTRNERPLERGVVETGKED 60
           M FM+IK+FCCF+FFSR+D ESKN+AWEAMGYRVDT+KRLN+TR ERPLERG++ETGKE 
Sbjct: 150 MVFMIIKMFCCFKFFSRIDGESKNIAWEAMGYRVDTRKRLNDTRKERPLERGLIETGKEH 209

Query: 61  DSTFLKSLSLKGLEATEDLRHGNAYKIKCDVAIVGSGCGGGVAAAVLARSGLKVVVLEKG 120
           DST L+SLS+KGL  TED +HG+ Y IKCDV IVGSGCGGGVAAA+LA+SGLKVVVLEKG
Sbjct: 210 DSTLLRSLSMKGLAVTEDQKHGSTYTIKCDVVIVGSGCGGGVAAAILAKSGLKVVVLEKG 269

Query: 121 NYFVPEDYSSLEGPSMNELYELGGLKSTVDGKVMLLAGSTVGGGSAINWSASIRTPKSVL 180
           NYFVPEDYSSLEGPSM+ELYE GGLKST+DGKVMLLAG+TVGGGSAINWSASIRTPK VL
Sbjct: 270 NYFVPEDYSSLEGPSMDELYETGGLKSTLDGKVMLLAGTTVGGGSAINWSASIRTPKPVL 329

Query: 181 DEWSTDRKIPFFGSFEYQSAMDAVCDRIGVTYGCSIESFQNQVFRKGCENLGLKVDSVPR 240
           +EWST  KIP FGSF+Y+SAMDAVCDRIGVT GC +ESFQNQV RKGCENLGLKVDSVPR
Sbjct: 330 EEWSTIHKIPLFGSFDYRSAMDAVCDRIGVTEGCRLESFQNQVLRKGCENLGLKVDSVPR 389

Query: 241 NSSVNHYCGSCSYGCRVGDKKGTDSTWLVDAVESGAVILTGCKADKLILENTNSTNRNKK 300
           NSS +HYCGSC YGCRVGDKKGTDSTWLVDAVESGAVILTGC+ADKL+LENTN+T+RNK+
Sbjct: 390 NSSKDHYCGSCCYGCRVGDKKGTDSTWLVDAVESGAVILTGCRADKLVLENTNNTSRNKR 449

Query: 301 CSGVVATLLSKTVTKKLHIEAKATISACGSLLTPPLLIASGLNNKNIGRNLHLHPVTLAW 360
           C GV+AT LSK VTKKL+IEAKATISACGSLLTPPLLIASGL NKNIG NLHLHPV LAW
Sbjct: 450 CLGVIATPLSKNVTKKLYIEAKATISACGSLLTPPLLIASGLRNKNIGNNLHLHPVILAW 509

Query: 361 GYFPERLSDFTGKIHEGGLITSIHKVVSEDSNSHAIIETPALGPASFAALFPWTSGRDMK 420
           GYFPE+LSDFTGKI+EGGLITS+H VVS+DSNSHAII+TPALGPASFAA+FPWTSGRDMK
Sbjct: 510 GYFPEQLSDFTGKIYEGGLITSLHNVVSQDSNSHAIIQTPALGPASFAAMFPWTSGRDMK 569

Query: 421 DAMAKYARTSHLFALVRDRGAGEVKVNADVKYRLDQTDKENLKIGLRQALRILIAAGATE 480
           DAM KYART+HLFALVRD+GAGE+K  A+VKYRLDQTDKENL+IGLRQALRILIAAGATE
Sbjct: 570 DAMVKYARTAHLFALVRDKGAGEIKGKANVKYRLDQTDKENLRIGLRQALRILIAAGATE 629

Query: 481 VGTYRSDGQRIRCEGANKEDIEEFLDTVEAVGGPSSREEHWTMYCSAHQMSSCRMGSTEE 540
           VGTYRSDGQR R  G NKED+EEFLD+VEA GGP SRE++W +YCSAHQMSSCRMGSTEE
Sbjct: 630 VGTYRSDGQRFRFHGTNKEDLEEFLDSVEAAGGPFSREKYWNIYCSAHQMSSCRMGSTEE 689

Query: 541 EGAVDENGESWEAEGLFVCDGSVLPTAVGVNPMITIQSTAYCISNRIA 589
           EGAVDENGESWEA+ LFVCDGSVLPTAVGVNPMITIQSTAYCIS ++A
Sbjct: 690 EGAVDENGESWEAKSLFVCDGSVLPTAVGVNPMITIQSTAYCISKKLA 737

BLAST of Sed0012772 vs. ExPASy Swiss-Prot
Match: B5WWZ9 (Long-chain-alcohol oxidase FAO2 OS=Lotus japonicus OX=34305 GN=FAO2 PE=2 SV=1)

HSP 1 Score: 793.1 bits (2047), Expect = 2.1e-228
Identity = 392/598 (65.55%), Postives = 475/598 (79.43%), Query Frame = 0

Query: 1   MAFMVIKVFCCFRFFSRVDEESKNVAWEAMGYRVDTQKRLNNTRNERPLERGVVETGKED 60
           + F++ K+ C +  FSR D    N  W+A+GY+VDT+++L  T+ +RPL+ G++ET  E 
Sbjct: 154 LVFVLTKLVCFYNLFSRADVNGHNPIWKAIGYQVDTREKL--TQKKRPLQEGLIETMYET 213

Query: 61  DSTFLKSLSLKGLEATEDLRHGNAYKIKCDVAIVGSGCGGGVAAAVLARSGLKVVVLEKG 120
           DST ++SL+ KGLE TEDL   N YKIKCD  IVGSGCGGGVAAAVLA SG KV++LEKG
Sbjct: 214 DSTLIQSLTEKGLEVTEDLEQ-NMYKIKCDAVIVGSGCGGGVAAAVLANSGHKVIILEKG 273

Query: 121 NYFVPEDYSSLEGPSMNELYELGGLKSTVDGKVMLLAGSTVGGGSAINWSASIRTPKSVL 180
            YFV  DYSSLEGPSMNELYE GG+  ++DGK+M+LAGST+GG SAINWSA IRTP SVL
Sbjct: 274 EYFVSHDYSSLEGPSMNELYESGGILPSLDGKMMILAGSTLGGXSAINWSACIRTPDSVL 333

Query: 181 DEWSTDRKIPFFGSFEYQSAMDAVCDRIGVTYGCSIESFQNQVFRKGCENLGLKVDSVPR 240
            EWS   KIP F S +YQSAMD VC RIGVT  C+ ESFQNQ+ R+GC  +G KV+ V  
Sbjct: 334 REWSEKHKIPLFASPDYQSAMDTVCRRIGVTENCNKESFQNQILRQGCAKIGFKVEPVAI 393

Query: 241 NSSVNHYCGSCSYGCRVGDKKGTDSTWLVDAVESGAVILTGCKADKL--ILENTNSTNRN 300
           NSS +HYCGSC YGCR GDKKGT+STWLVDAV +GAVILTGCKA+KL   L++ ++  + 
Sbjct: 394 NSSADHYCGSCCYGCRTGDKKGTESTWLVDAVGNGAVILTGCKAEKLNFTLKDGDNGTKR 453

Query: 301 KKCSGVVATLLSKT-VTKKLHIEAKATISACGSLLTPPLLIASGLNNKNIGRNLHLHPVT 360
           K CSGV+A+   ++ VTKKL IE+K TISACGSL TPPL+I+SGL N NIG+NLHLHP  
Sbjct: 454 KTCSGVIASATWRSKVTKKLQIESKVTISACGSLSTPPLMISSGLKNPNIGKNLHLHPCQ 513

Query: 361 LAWGYFPERLSDFTGKIHEGGLITSIHKVVSEDSNS--HAIIETPALGPASFAALFPWTS 420
            AWGYFPE +++F+G  +EGG+ITSIHKV  EDS S    IIE PALGP SF+AL PW S
Sbjct: 514 FAWGYFPEDMTNFSGNNYEGGIITSIHKVFEEDSTSTPRIIIEAPALGPGSFSALVPWVS 573

Query: 421 GRDMKDAMAKYARTSHLFALVRDRGAGEVKVNADVKYRLDQTDKENLKIGLRQALRILIA 480
           G D+K+ M KYART++LFALVRD G+GEVK    + Y+LD+ D+E+L+ GLR+ALRIL+A
Sbjct: 574 GLDVKERMVKYARTANLFALVRDHGSGEVKAEGRISYKLDKIDRESLQTGLRKALRILVA 633

Query: 481 AGATEVGTYRSDGQRIRCEGANKEDIEEFLDTVEAVGGPSSREEHWTMYCSAHQMSSCRM 540
           AGA EVGTYRSDGQRI+C G  + D+EEFLD+V  VGGPSSR E WT++ SAHQM+SCRM
Sbjct: 634 AGAVEVGTYRSDGQRIKCRGIKESDLEEFLDSVRVVGGPSSRNEVWTVFTSAHQMTSCRM 693

Query: 541 GSTEEEGAVDENGESWEAEGLFVCDGSVLPTAVGVNPMITIQSTAYCISNRIAHSFNK 594
            +TEEEGAVDENGESWEA+GL+VCDGSVLP+AVGVNPMITIQSTAYCI++ IA S  K
Sbjct: 694 SATEEEGAVDENGESWEAKGLYVCDGSVLPSAVGVNPMITIQSTAYCIASNIAESLKK 748

BLAST of Sed0012772 vs. ExPASy Swiss-Prot
Match: B5WWZ8 (Long-chain-alcohol oxidase FAO1 OS=Lotus japonicus OX=34305 GN=FAO1 PE=1 SV=1)

HSP 1 Score: 758.4 bits (1957), Expect = 5.9e-218
Identity = 372/592 (62.84%), Postives = 455/592 (76.86%), Query Frame = 0

Query: 1   MAFMVIKVFCCFRFFSRVDEESKNVAWEAMGYRVDTQKRLNNTRNERPLERGVVETGKED 60
           +AF  +KV C F +F+ VDE   N AW+A+GY V   ++L N+ N R LE+G+VET  E 
Sbjct: 150 LAFAYLKVLCLFAYFTWVDENGDNPAWKAIGYEVSADEKLPNSSNGRLLEKGIVETMHET 209

Query: 61  DSTFLKSLSLKGLEATEDLRHGNAYKIKCDVAIVGSGCGGGVAAAVLARSGLKVVVLEKG 120
           +ST  +SL+ KGL  T D R+ N  K+KCD  +VGSGCGGGVAA+VL+ +G KVVVLEKG
Sbjct: 210 NSTLQQSLTEKGLNVTLDSRN-NILKVKCDAIVVGSGCGGGVAASVLSGAGHKVVVLEKG 269

Query: 121 NYFVPEDYSSLEGPSMNELYELGGLKSTVDGKVMLLAGSTVGGGSAINWSASIRTPKSVL 180
           NYF P DYSSLEGPSM++LYE GG+ ++VD +++LLAGSTVGGGSA+NWSA I+TP  VL
Sbjct: 270 NYFAPRDYSSLEGPSMDQLYETGGILASVDSRILLLAGSTVGGGSAVNWSACIKTPHEVL 329

Query: 181 DEWSTDRKIPFFGSFEYQSAMDAVCDRIGVTYGCSIESFQNQVFRKGCENLGLKVDSVPR 240
            EWS +  +  F + EY SAM+ VC+RIGVT  C+ E FQNQV RKGC+NLGLKVD VPR
Sbjct: 330 KEWSENHNLSLFSTSEYLSAMETVCERIGVTESCTHEGFQNQVLRKGCQNLGLKVDYVPR 389

Query: 241 NSSVNHYCGSCSYGCRVGDKKGTDSTWLVDAVESGAVILTGCKADKLILENT-NSTNRNK 300
           NSS NH+CGSC YGC  G+K+GT  TWLVDAVE+GAVI+TGCKA++ +LEN  N + R K
Sbjct: 390 NSSGNHFCGSCGYGCPKGEKQGTQDTWLVDAVENGAVIITGCKAERFLLENNRNGSGRKK 449

Query: 301 KCSGVVATLLSKTVTKKLHIEAKATISACGSLLTPPLLIASGLNNKNIGRNLHLHPVTLA 360
           KC GV+A  LS  VT KL IEAK TISA G+LLTPPL+I+SGL NKNIG+NLHLHPV + 
Sbjct: 450 KCLGVLAKALSSRVTMKLQIEAKVTISAGGALLTPPLMISSGLKNKNIGKNLHLHPVLMT 509

Query: 361 WGYFPERLSDFTGKIHEGGLITSIHKVVSEDSNSH----AIIETPALGPASFAALFPWTS 420
           WGYFPE  S+  GKI EGG+ITS+HKV S DSNS+    AIIETP+LGP SFA+L PW S
Sbjct: 510 WGYFPESTSELKGKIFEGGIITSVHKVPSRDSNSYSDTRAIIETPSLGPGSFASLCPWES 569

Query: 421 GRDMKDAMAKYARTSHLFALVRDRGAGEVKVNADVKYRLDQTDKENLKIGLRQALRILIA 480
           G D K+ M  Y RT+HL  ++RD+  G+V     V Y+L   DKEN+K GL+QALRIL A
Sbjct: 570 GLDFKERMLNYPRTAHLITIIRDKACGQVTTEGRVSYKLSAFDKENMKCGLQQALRILKA 629

Query: 481 AGATEVGTYRSDGQRIRCEGANKEDIEEFLDTVEAVGGPSSREEHWTMYCSAHQMSSCRM 540
           AGA EVGT+RSDGQR++C   ++ ++ EF+D+V  + G  S  E+W +Y SAHQM SCRM
Sbjct: 630 AGAVEVGTHRSDGQRVKCGEVSENEMAEFIDSVCPMEGALSPGENWNIYTSAHQMGSCRM 689

Query: 541 GSTEEEGAVDENGESWEAEGLFVCDGSVLPTAVGVNPMITIQSTAYCISNRI 588
           G  E+EGAVDENGESWEA+GLFVCD S+LPTAVGVNPMITIQSTAYCISNR+
Sbjct: 690 GMNEKEGAVDENGESWEAQGLFVCDASLLPTAVGVNPMITIQSTAYCISNRV 740

BLAST of Sed0012772 vs. ExPASy Swiss-Prot
Match: Q9LW56 (Long-chain-alcohol oxidase FAO3 OS=Arabidopsis thaliana OX=3702 GN=FAO3 PE=1 SV=1)

HSP 1 Score: 748.4 bits (1931), Expect = 6.1e-215
Identity = 371/595 (62.35%), Postives = 456/595 (76.64%), Query Frame = 0

Query: 2   AFMVIKVFCCFRFFSRVDEESKNVAWEAMGYRVD-TQKRLNNTRNERPLERGVVETGKED 61
           AF+ IKV   F FFSRV+   +N AWEA+GYRV+  + + + T NERPLE+G+VET +E 
Sbjct: 146 AFVYIKVAFLFCFFSRVNPNGENPAWEAIGYRVNPDENKPSETHNERPLEKGIVETMEET 205

Query: 62  DSTFLKSLSLKGLEATEDLRHGNAYKIKCDVAIVGSGCGGGVAAAVLARSGLKVVVLEKG 121
           + T L+SL+ KGLEA  D  H +A +IKCDV +VGSG GGGVAA+VLA+SGLKVVVLEKG
Sbjct: 206 EQTLLESLAHKGLEAVLDTEH-DAIRIKCDVVVVGSGSGGGVAASVLAKSGLKVVVLEKG 265

Query: 122 NYFVPEDYSSLEGPSMNELYELGGLKSTVDGKVMLLAGSTVGGGSAINWSASIRTPKSVL 181
           +YF P ++   EGP +++LYE GG+  +VDG  M+LAG+TVGGGSA+NWSA I+TPKSVL
Sbjct: 266 SYFTPSEHRPFEGPGLDKLYENGGILPSVDGSFMVLAGATVGGGSAVNWSACIKTPKSVL 325

Query: 182 DEWSTDRKIPFFGSFEYQSAMDAVCDRIGVTYGCSIESFQNQVFRKGCENLGLKVDSVPR 241
            EWS D+ IP FG+ EY +AM+ V  R+GVT  C +ESFQNQ+ RKGCENLG  V++VPR
Sbjct: 326 QEWSEDQNIPLFGTKEYLTAMEVVWKRMGVTEKCELESFQNQILRKGCENLGFNVENVPR 385

Query: 242 NSSVNHYCGSCSYGCRVGDKKGTDSTWLVDAVESGAVILTGCKADKLILENTNSTNRNK- 301
           NSS +HYCGSC YGCR GDKKG+D TWLVDAV  GAVILTGCKA++ ILE   S    K 
Sbjct: 386 NSSESHYCGSCGYGCRQGDKKGSDRTWLVDAVGHGAVILTGCKAERFILEKNGSNKGGKQ 445

Query: 302 -KCSGVVATLLSKTVTKKLHIEAKATISACGSLLTPPLLIASGLNNKNIGRNLHLHPVTL 361
            KC GV+A  L+  + K L IEAK T+SA G+LLTPPL+I+SGL N+NIG+NLHLHPV +
Sbjct: 446 MKCLGVMAKSLNGNIAKMLKIEAKVTVSAGGALLTPPLMISSGLRNRNIGKNLHLHPVLM 505

Query: 362 AWGYFPERLS---DFTGKIHEGGLITSIHKVVSEDSNSHAIIETPALGPASFAALFPWTS 421
           AWGYFP++ S    F G  +EGG+ITS+ KV+SEDS   AIIETP LGP SF+ L PWTS
Sbjct: 506 AWGYFPDKESSNISFKGNSYEGGIITSVSKVLSEDSEVRAIIETPQLGPGSFSVLTPWTS 565

Query: 422 GRDMKDAMAKYARTSHLFALVRDRGAGEVKVNADVKYRLDQTDKENLKIGLRQALRILIA 481
           G DMK  MA+Y+RT+ L  +VRDRG+GEVK    + Y +D+TD++NLK GLR++LRILIA
Sbjct: 566 GLDMKKRMARYSRTASLITIVRDRGSGEVKTEGRINYTVDKTDRDNLKAGLRESLRILIA 625

Query: 482 AGATEVGTYRSDGQRIRCEGANKEDIEEFLDTVEAVGGPSSREEHWTMYCSAHQMSSCRM 541
           AGA EVGT+RSDGQR+ C+G N+  I+EFLD+V    G     E W +Y SAHQM SCR+
Sbjct: 626 AGAEEVGTHRSDGQRLICKGVNENSIQEFLDSVSTEEGAKGMTEKWNVYSSAHQMGSCRI 685

Query: 542 GSTEEEGAVDENGESWEAEGLFVCDGSVLPTAVGVNPMITIQSTAYCISNRIAHS 591
           G  E+EGA+D NGESWEAE LFVCD S LP+AVGVNPMIT+ STAYCIS RIA S
Sbjct: 686 GENEKEGAIDLNGESWEAEKLFVCDASALPSAVGVNPMITVMSTAYCISTRIAKS 739

BLAST of Sed0012772 vs. ExPASy Swiss-Prot
Match: Q94BP3 (Long-chain-alcohol oxidase FAO4B OS=Arabidopsis thaliana OX=3702 GN=FAO4B PE=2 SV=2)

HSP 1 Score: 743.0 bits (1917), Expect = 2.5e-213
Identity = 372/597 (62.31%), Postives = 454/597 (76.05%), Query Frame = 0

Query: 1   MAFMVIKVFCCFRFFSRVDEESKNVAWEAMGYRVDTQKRLNNTRNE-----RPLERGVVE 60
           + F + K +  F FFS+ DE  KN A EA+GY +D  +R +N ++E     RPLE+G++E
Sbjct: 146 ITFFLAKFYTLFYFFSQTDENLKNPALEAIGYCIDGTERSSNKKSEADEKRRPLEKGIIE 205

Query: 61  TGKEDDSTFLKSLSLKGLEATEDLRHGNAYKIKCDVAIVGSGCGGGVAAAVLARSGLKVV 120
           T  E D T  +SL+ KG+    D    N Y+I+CD  +VGSG GGGVAAA LA++GLKV+
Sbjct: 206 TMHESDVTITQSLTEKGVHVARD-DGDNVYRIRCDAVVVGSGSGGGVAAANLAKAGLKVL 265

Query: 121 VLEKGNYFVPEDYSSLEGPSMNELYELGGLKSTVDGKVMLLAGSTVGGGSAINWSASIRT 180
           VLEKGNYF   DYS LE PSM ELYE GGL +TVDGK MLLAGS VGGG+A+NWSASIRT
Sbjct: 266 VLEKGNYFTAHDYSGLEVPSMLELYEKGGLLTTVDGKFMLLAGSAVGGGTAVNWSASIRT 325

Query: 181 PKSVLDEWSTDRKIPFFGSFEYQSAMDAVCDRIGVTYGCSIESFQNQVFRKGCENLGLKV 240
           P  VL EWS   KI FFGS EYQSAMD V  RIGVT  C    FQNQV RKGCE LGL+V
Sbjct: 326 PDHVLQEWSEGSKIKFFGSQEYQSAMDEVTIRIGVTERCVKHGFQNQVLRKGCERLGLQV 385

Query: 241 DSVPRNSSVNHYCGSCSYGCRVGDKKGTDSTWLVDAVESGAVILTGCKADKLIL-ENTNS 300
           +SVPRNS  +HYCG C YGCR G K GTD TWLVDAVE+GAVILTG KA++ +L +NT+S
Sbjct: 386 ESVPRNSPEDHYCGLCGYGCRAGAKNGTDQTWLVDAVENGAVILTGIKAERFVLVDNTSS 445

Query: 301 TN-RNKKCSGVVATLLSKTVTKKLHIEAKATISACGSLLTPPLLIASGLNNKNIGRNLHL 360
           +N R K+C GV A+ +   + KK  IEA+ T+S+ GSLLTPPL+++SGL N NIGRNL L
Sbjct: 446 SNERKKRCVGVFASSVGGKIGKKFIIEARVTVSSAGSLLTPPLMLSSGLKNPNIGRNLKL 505

Query: 361 HPVTLAWGYFPERLSDFTGKIHEGGLITSIHKVVSEDSNSHAIIETPALGPASFAALFPW 420
           HPV + WGYFPE+ S+F+GK++EGG+ITS+H +   +S   AI+E P +GPAS+A L PW
Sbjct: 506 HPVLMTWGYFPEKDSEFSGKMYEGGIITSVHHMNDTESGCKAILENPLIGPASYAGLSPW 565

Query: 421 TSGRDMKDAMAKYARTSHLFALVRDRGAGEVKVNADVKYRLDQTDKENLKIGLRQALRIL 480
            SG D+K+ M KY RT+HLFALVRD G+GEV +  +V YR  + D+ENL+ GLRQALR+ 
Sbjct: 566 VSGPDLKERMIKYGRTAHLFALVRDLGSGEVMMENEVTYRTTKKDRENLRAGLRQALRVS 625

Query: 481 IAAGATEVGTYRSDGQRIRCEGANKEDIEEFLDTVEAVGGPSSREEHWTMYCSAHQMSSC 540
           +AAGA EVGTYRSDGQ+++CE   KE +EEFLD V+AVGG  ++ E+WT Y SAHQM SC
Sbjct: 626 VAAGAVEVGTYRSDGQKMKCEAITKEAMEEFLDEVDAVGGVGTKGEYWTTYFSAHQMGSC 685

Query: 541 RMGSTEEEGAVDENGESWEAEGLFVCDGSVLPTAVGVNPMITIQSTAYCISNRIAHS 591
           RMG T EEGA+DENGESWEAEGLFVCDGS+LP+AVGVNPMITIQSTAYCIS++I  S
Sbjct: 686 RMGVTAEEGALDENGESWEAEGLFVCDGSILPSAVGVNPMITIQSTAYCISSKIVDS 741

BLAST of Sed0012772 vs. ExPASy Swiss-Prot
Match: Q9ZWB9 (Long-chain-alcohol oxidase FAO1 OS=Arabidopsis thaliana OX=3702 GN=FAO1 PE=3 SV=2)

HSP 1 Score: 709.5 bits (1830), Expect = 3.1e-203
Identity = 351/599 (58.60%), Postives = 444/599 (74.12%), Query Frame = 0

Query: 1   MAFMVIKVFCCFRFFSRVDEESKNVAWEAMGYRVDTQKRLNNTRNERPLERGVVETGKED 60
           + FM+IK    F +F+  +E S+N  W+A+ Y V+  +  +  + ERPL+ G++ET KED
Sbjct: 159 IGFMMIKAIFLFYYFTWTNENSENPVWDAINYSVEIGENEDMEQKERPLDEGIIETAKED 218

Query: 61  DSTFLKSLSLKGLEATEDLRHGNAYKIKCDVAIVGSGCGGGVAAAVLARSGLKVVVLEKG 120
           + T  + +  KGL+ TED R  + YKI+CD  +VGSGCGGGVAAA+LA+SGL+VVV+EKG
Sbjct: 219 EMTIKQRMINKGLKVTED-RERDTYKIECDAVVVGSGCGGGVAAAILAKSGLRVVVIEKG 278

Query: 121 NYFVPEDYSSLEGPSMNELYELGGLKSTVDGKVMLLAGSTVGGGSAINWSASIRTPKSVL 180
           NYF P DYS+LEGPSM EL+E   L  T DG+   +AGSTVGGGS +NW+AS++TP +++
Sbjct: 279 NYFAPRDYSALEGPSMFELFESNSLMMTHDGRFRFMAGSTVGGGSVVNWAASLKTPDAII 338

Query: 181 DEWSTDRKIPFFGSFEYQSAMDAVCDRIGVTYGCSIESFQNQVFRKGCENLGLKVDSVPR 240
           +EWS  R I  + S +Y++AM  VC R+GVT     E FQNQ+ RKGCE LGL V  VPR
Sbjct: 339 EEWSVHRGISIYSSEKYKAAMGIVCKRLGVTEKIIREGFQNQILRKGCEKLGLDVTIVPR 398

Query: 241 NSSVNHYCGSCSYGCRVGDKKGTDSTWLVDAVESGAVILTGCKADKLILENTNS-----T 300
           NS+  HYCGSCSYGC  G+K+GTDSTWLVDAV + AVILT CKA+KLIL + ++     +
Sbjct: 399 NSTEKHYCGSCSYGCPTGEKRGTDSTWLVDAVNNNAVILTQCKAEKLILADNDANKREES 458

Query: 301 NRNKKCSGVVATLLSKTVTKKLHIEAKATISACGSLLTPPLLIASGLNNKNIGRNLHLHP 360
            R K+C GV A+L  +T  KKL I AK TI ACGSL TP LL +SGL N NI R LH+HP
Sbjct: 459 GRRKRCLGVAASLSHQT-RKKLQINAKVTIVACGSLKTPGLLASSGLKNSNISRGLHIHP 518

Query: 361 VTLAWGYFPERLSDFTGKIHEGGLITSIHKVVSEDSNSHAI-IETPALGPASFAALFPWT 420
           + +AWGYFPE+ S+  G  HEG ++TS+H V   DS +  I +ETPA+GP +FAAL PW 
Sbjct: 519 IMMAWGYFPEKNSELEGAAHEGEIVTSLHYVHPMDSTTPNITLETPAIGPGTFAALTPWV 578

Query: 421 SGRDMKDAMAKYARTSHLFALVRDRGAGEVKVNADVKYRLDQTDKENLKIGLRQALRILI 480
           SG DMK+ MAKYART+H+FA+VRD G GEVK +  VKYRL + D+ENL IGL+QALRIL+
Sbjct: 579 SGSDMKERMAKYARTAHIFAMVRDEGVGEVKGDI-VKYRLTKADEENLTIGLKQALRILV 638

Query: 481 AAGATEVGTYRSDGQRIRCEGANKEDIEEFLDTVEAVGGPSSREEHWTMYCSAHQMSSCR 540
           AAGA EVGTYRSDGQR++C+G  ++D+E FLDTV A  G  S  +HWT   +AHQ+  CR
Sbjct: 639 AAGAAEVGTYRSDGQRMKCDGIKQKDLEAFLDTVNAPPGVVSMSKHWTQSFTAHQIGCCR 698

Query: 541 MGSTEEEGAVDENGESWEAEGLFVCDGSVLPTAVGVNPMITIQSTAYCISNRIAHSFNK 594
           MG+TE+EGA+D  GESWEAE L+VCD SVLPTA+GVNPMIT+QSTAYCISNRIA    K
Sbjct: 699 MGATEKEGAIDGKGESWEAEDLYVCDASVLPTALGVNPMITVQSTAYCISNRIAELMKK 754

BLAST of Sed0012772 vs. ExPASy TrEMBL
Match: A0A6J1HWN7 (Long-chain-alcohol oxidase OS=Cucurbita maxima OX=3661 GN=LOC111466966 PE=3 SV=1)

HSP 1 Score: 1044.3 bits (2699), Expect = 2.0e-301
Identity = 504/592 (85.14%), Postives = 551/592 (93.07%), Query Frame = 0

Query: 1   MAFMVIKVFCCFRFFSRVDEESKNVAWEAMGYRVDTQKRLNNTRNERPLERGVVETGKED 60
           M FM+IK+FCCF+FFSR+D ESKN+AWEAMGY VDT+KRLN++R ERPLERG++ETGKED
Sbjct: 147 MVFMIIKMFCCFKFFSRIDRESKNIAWEAMGYHVDTRKRLNDSRKERPLERGLIETGKED 206

Query: 61  DSTFLKSLSLKGLEATEDLRHGNAYKIKCDVAIVGSGCGGGVAAAVLARSGLKVVVLEKG 120
           DST L+SLS+KGL  TED +HG+ Y IKCDV IVGSGCGGGVAAAVLA+SGLKVVVLEKG
Sbjct: 207 DSTLLRSLSMKGLAVTEDQKHGSTYTIKCDVVIVGSGCGGGVAAAVLAKSGLKVVVLEKG 266

Query: 121 NYFVPEDYSSLEGPSMNELYELGGLKSTVDGKVMLLAGSTVGGGSAINWSASIRTPKSVL 180
           NYFVPEDYSSLEGPSMNELYE GGLKST+DGKVMLLAG+TVGGGSAINWSASIRTPK VL
Sbjct: 267 NYFVPEDYSSLEGPSMNELYETGGLKSTLDGKVMLLAGTTVGGGSAINWSASIRTPKPVL 326

Query: 181 DEWSTDRKIPFFGSFEYQSAMDAVCDRIGVTYGCSIESFQNQVFRKGCENLGLKVDSVPR 240
           +EWST  KIP FGSF+YQSAMDAVCDRIGVT GC +ESFQNQV RKGCENLGLKVDSVPR
Sbjct: 327 EEWSTIHKIPLFGSFDYQSAMDAVCDRIGVTEGCRLESFQNQVLRKGCENLGLKVDSVPR 386

Query: 241 NSSVNHYCGSCSYGCRVGDKKGTDSTWLVDAVESGAVILTGCKADKLILENTNSTNRNKK 300
           NSS +HYCGSC YGCRVGDKKGTDSTWLVDAVESGAVILTGC+ADKL+LENTN+T+RNK+
Sbjct: 387 NSSKDHYCGSCCYGCRVGDKKGTDSTWLVDAVESGAVILTGCRADKLVLENTNNTSRNKR 446

Query: 301 CSGVVATLLSKTVTKKLHIEAKATISACGSLLTPPLLIASGLNNKNIGRNLHLHPVTLAW 360
           C GV+AT LSK VTKKLHIEAKATISACGSLLTPPLLIASGL NKNIG NLHLHPV LAW
Sbjct: 447 CLGVIATPLSKNVTKKLHIEAKATISACGSLLTPPLLIASGLRNKNIGSNLHLHPVILAW 506

Query: 361 GYFPERLSDFTGKIHEGGLITSIHKVVSEDSNSHAIIETPALGPASFAALFPWTSGRDMK 420
           GYFPE+LSDFTGKI+EGGLITS+H VVS+DSNSHAII+TPALGPASFAA+FPWTSGRDMK
Sbjct: 507 GYFPEQLSDFTGKIYEGGLITSLHNVVSKDSNSHAIIQTPALGPASFAAMFPWTSGRDMK 566

Query: 421 DAMAKYARTSHLFALVRDRGAGEVKVNADVKYRLDQTDKENLKIGLRQALRILIAAGATE 480
           DA+ KYART+HLFALVRDRGAGE+KV A+VKYRLDQTDKENL+IGLRQALRILIAAGATE
Sbjct: 567 DAVVKYARTAHLFALVRDRGAGEIKVKANVKYRLDQTDKENLRIGLRQALRILIAAGATE 626

Query: 481 VGTYRSDGQRIRCEGANKEDIEEFLDTVEAVGGPSSREEHWTMYCSAHQMSSCRMGSTEE 540
           VGTYRSDGQR +  G NKED+EEF D+VEA GGP SRE++W +YCSAHQMSSCRMGSTEE
Sbjct: 627 VGTYRSDGQRFQFHGTNKEDLEEFFDSVEAAGGPFSREKYWNIYCSAHQMSSCRMGSTEE 686

Query: 541 EGAVDENGESWEAEGLFVCDGSVLPTAVGVNPMITIQSTAYCISNRIAHSFN 593
           EGAVDENGESWEA+GLFVCDGSVLPTAVGVNPMITIQSTAYCIS ++A S N
Sbjct: 687 EGAVDENGESWEAKGLFVCDGSVLPTAVGVNPMITIQSTAYCISKKLAESLN 738

BLAST of Sed0012772 vs. ExPASy TrEMBL
Match: A0A6J1HG83 (Long-chain-alcohol oxidase OS=Cucurbita moschata OX=3662 GN=LOC111464000 PE=3 SV=1)

HSP 1 Score: 1041.2 bits (2691), Expect = 1.7e-300
Identity = 502/592 (84.80%), Postives = 551/592 (93.07%), Query Frame = 0

Query: 1   MAFMVIKVFCCFRFFSRVDEESKNVAWEAMGYRVDTQKRLNNTRNERPLERGVVETGKED 60
           M FM+IK+FCCF+FFS +D ESKN+AWEAMGYRVDT+KRLN+TR ERPLERGV+E GKED
Sbjct: 147 MVFMIIKMFCCFKFFSGIDRESKNIAWEAMGYRVDTRKRLNDTRKERPLERGVIEAGKED 206

Query: 61  DSTFLKSLSLKGLEATEDLRHGNAYKIKCDVAIVGSGCGGGVAAAVLARSGLKVVVLEKG 120
           DST L+SLS+KGL  TED +HG+ Y IKCDV IVGSGCGGGVAAAVLA+SGLKVVVLEKG
Sbjct: 207 DSTLLQSLSMKGLAVTEDPKHGSTYTIKCDVVIVGSGCGGGVAAAVLAKSGLKVVVLEKG 266

Query: 121 NYFVPEDYSSLEGPSMNELYELGGLKSTVDGKVMLLAGSTVGGGSAINWSASIRTPKSVL 180
           NYFVPEDYSSLEGPSMNELYE GGLKST+DGKVMLLAG+TVGGGSAINWSASIRTPK VL
Sbjct: 267 NYFVPEDYSSLEGPSMNELYETGGLKSTIDGKVMLLAGTTVGGGSAINWSASIRTPKPVL 326

Query: 181 DEWSTDRKIPFFGSFEYQSAMDAVCDRIGVTYGCSIESFQNQVFRKGCENLGLKVDSVPR 240
           +EWST  KIP FGSF+Y+SAMDAVCDRIGVT GC +ESFQNQV RKGCENLGLKVDSVPR
Sbjct: 327 EEWSTIHKIPLFGSFDYRSAMDAVCDRIGVTEGCRLESFQNQVLRKGCENLGLKVDSVPR 386

Query: 241 NSSVNHYCGSCSYGCRVGDKKGTDSTWLVDAVESGAVILTGCKADKLILENTNSTNRNKK 300
           NSS +HYCGSC YGCRVGDKKGTD+TWLVDAVESGAVILTGC+ADKL+LENTN+T+RNK+
Sbjct: 387 NSSKDHYCGSCCYGCRVGDKKGTDATWLVDAVESGAVILTGCRADKLVLENTNNTSRNKR 446

Query: 301 CSGVVATLLSKTVTKKLHIEAKATISACGSLLTPPLLIASGLNNKNIGRNLHLHPVTLAW 360
           C GV+AT+LSK VTKKL+IEAKATISACGSLLTPPLLIASGL NKNIG NLHLHPV LAW
Sbjct: 447 CLGVIATMLSKNVTKKLYIEAKATISACGSLLTPPLLIASGLRNKNIGSNLHLHPVILAW 506

Query: 361 GYFPERLSDFTGKIHEGGLITSIHKVVSEDSNSHAIIETPALGPASFAALFPWTSGRDMK 420
           GYFPE+LSDFTGKI+EGGLITS+H VVS+DSNSHAII+TPALGPASFAA+FPWTSGRDMK
Sbjct: 507 GYFPEQLSDFTGKIYEGGLITSLHNVVSKDSNSHAIIQTPALGPASFAAMFPWTSGRDMK 566

Query: 421 DAMAKYARTSHLFALVRDRGAGEVKVNADVKYRLDQTDKENLKIGLRQALRILIAAGATE 480
           DAM KYART+HLFALVRD+GAGE+K  A+VKYRLDQTDKENL+IGLRQALRILIAAGATE
Sbjct: 567 DAMVKYARTAHLFALVRDKGAGEIKAKANVKYRLDQTDKENLRIGLRQALRILIAAGATE 626

Query: 481 VGTYRSDGQRIRCEGANKEDIEEFLDTVEAVGGPSSREEHWTMYCSAHQMSSCRMGSTEE 540
           VGTYRS+GQR R  G NKED+EEFLD+VEA GGP SRE++W +YCSAHQMSSCRMGSTEE
Sbjct: 627 VGTYRSNGQRFRFHGTNKEDLEEFLDSVEAAGGPFSREKYWNIYCSAHQMSSCRMGSTEE 686

Query: 541 EGAVDENGESWEAEGLFVCDGSVLPTAVGVNPMITIQSTAYCISNRIAHSFN 593
           EGAVDENGESWEA+GLFVCDGSVLPTAVGVNPMITIQSTAYCIS ++A S N
Sbjct: 687 EGAVDENGESWEAKGLFVCDGSVLPTAVGVNPMITIQSTAYCISKKLAESLN 738

BLAST of Sed0012772 vs. ExPASy TrEMBL
Match: A0A5A7T3R3 (Long-chain-alcohol oxidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold36G001660 PE=3 SV=1)

HSP 1 Score: 1026.5 bits (2653), Expect = 4.3e-296
Identity = 497/592 (83.95%), Postives = 545/592 (92.06%), Query Frame = 0

Query: 1   MAFMVIKVFCCFRFFSRVDEESKNVAWEAMGYRVDTQKRLNNTRNERPLERGVVETGKED 60
           M FM+IK+FCCF+FFSR+DE +KNVAW+AMGY VDT+K+LN+T+ ERPLERG++ETG ED
Sbjct: 147 MTFMIIKIFCCFKFFSRIDEGTKNVAWKAMGYEVDTRKQLNHTQKERPLERGMIETGNED 206

Query: 61  DSTFLKSLSLKGLEATEDLRHGNAYKIKCDVAIVGSGCGGGVAAAVLARSGLKVVVLEKG 120
           DST  +SLS KGLE  ED +HGNAYKIKCDV IVGSGCGGGVAAAVLA+SGLKVVVLEKG
Sbjct: 207 DSTLFRSLSQKGLEVIEDKKHGNAYKIKCDVVIVGSGCGGGVAAAVLAKSGLKVVVLEKG 266

Query: 121 NYFVPEDYSSLEGPSMNELYELGGLKSTVDGKVMLLAGSTVGGGSAINWSASIRTPKSVL 180
           NYFV EDYSSLEGPSMNELYE GGLKST+DGKVMLLAG+TVGGGSAINWSASIRTPKSVL
Sbjct: 267 NYFVAEDYSSLEGPSMNELYESGGLKSTLDGKVMLLAGTTVGGGSAINWSASIRTPKSVL 326

Query: 181 DEWSTDRKIPFFGSFEYQSAMDAVCDRIGVTYGCSIESFQNQVFRKGCENLGLKVDSVPR 240
           DEWSTD KIP +GS +YQ AMDAVCDRIGVT GC +ESFQNQV RKGCENLGLKVDSV R
Sbjct: 327 DEWSTDHKIPLYGSIDYQLAMDAVCDRIGVTRGCRVESFQNQVLRKGCENLGLKVDSVSR 386

Query: 241 NSSVNHYCGSCSYGCRVGDKKGTDSTWLVDAVESGAVILTGCKADKLILENTNSTNRNKK 300
           NSS +HYCGSC YGCRVGDKKGTD+TWLVDAV+ GAVILTGC ADK ILENTN+T++ KK
Sbjct: 387 NSSEDHYCGSCCYGCRVGDKKGTDTTWLVDAVKCGAVILTGCHADKFILENTNNTSKKKK 446

Query: 301 CSGVVATLLSKTVTKKLHIEAKATISACGSLLTPPLLIASGLNNKNIGRNLHLHPVTLAW 360
           C GV+AT LSK VTKKLHIEAKATISACGSLLTPPLLIASGL NKNIG NLHLHPV+L W
Sbjct: 447 CIGVIATPLSKNVTKKLHIEAKATISACGSLLTPPLLIASGLRNKNIGTNLHLHPVSLVW 506

Query: 361 GYFPERLSDFTGKIHEGGLITSIHKVVSEDSNSHAIIETPALGPASFAALFPWTSGRDMK 420
           GYFPE LSDF GKI+EGGLIT +H VVS+ SNSHAIIETPA+GPASFAALFPWTSGR+MK
Sbjct: 507 GYFPEELSDFKGKIYEGGLITCLHNVVSKYSNSHAIIETPAVGPASFAALFPWTSGREMK 566

Query: 421 DAMAKYARTSHLFALVRDRGAGEVKVNADVKYRLDQTDKENLKIGLRQALRILIAAGATE 480
           DAM KY+RT+HLFALVRD+GAGE+KV A+VKYRL+QTDKENLKIGLRQALRILIAAGATE
Sbjct: 567 DAMVKYSRTAHLFALVRDQGAGEIKVKANVKYRLNQTDKENLKIGLRQALRILIAAGATE 626

Query: 481 VGTYRSDGQRIRCEGANKEDIEEFLDTVEAVGGPSSREEHWTMYCSAHQMSSCRMGSTEE 540
           +GTYRSDGQRIRC+G  KED+EEFLD+VEAVGGP SR+++WTMYCSAHQMSSCRMGST+E
Sbjct: 627 IGTYRSDGQRIRCQGTKKEDLEEFLDSVEAVGGPWSRQKYWTMYCSAHQMSSCRMGSTKE 686

Query: 541 EGAVDENGESWEAEGLFVCDGSVLPTAVGVNPMITIQSTAYCISNRIAHSFN 593
           EGAVDENGESWEAEGLFVCDGSVLPTAVGVNPMITIQSTAYCIS +IA S N
Sbjct: 687 EGAVDENGESWEAEGLFVCDGSVLPTAVGVNPMITIQSTAYCISKKIAESLN 738

BLAST of Sed0012772 vs. ExPASy TrEMBL
Match: A0A1S3BHB1 (Long-chain-alcohol oxidase OS=Cucumis melo OX=3656 GN=LOC103489850 PE=3 SV=1)

HSP 1 Score: 1024.2 bits (2647), Expect = 2.1e-295
Identity = 496/592 (83.78%), Postives = 544/592 (91.89%), Query Frame = 0

Query: 1   MAFMVIKVFCCFRFFSRVDEESKNVAWEAMGYRVDTQKRLNNTRNERPLERGVVETGKED 60
           M FM+IK+FCCF+FFSR+DE +KNVAW+AMGY VDT+K+LN+T+ ERPLERG++ETG ED
Sbjct: 147 MTFMIIKIFCCFKFFSRIDEGTKNVAWKAMGYEVDTRKQLNHTQKERPLERGMIETGNED 206

Query: 61  DSTFLKSLSLKGLEATEDLRHGNAYKIKCDVAIVGSGCGGGVAAAVLARSGLKVVVLEKG 120
           DST  +SLS KGLE  ED +HGNAYKIKCDV IVGSGCGGGVAAAVLA+SGLKVVVLEKG
Sbjct: 207 DSTLFRSLSQKGLEVIEDKKHGNAYKIKCDVVIVGSGCGGGVAAAVLAKSGLKVVVLEKG 266

Query: 121 NYFVPEDYSSLEGPSMNELYELGGLKSTVDGKVMLLAGSTVGGGSAINWSASIRTPKSVL 180
           NYFV EDYSSLEGPSMNELYE GGLKST+DGKVMLLAG+TVGGGSAINWSASIRTPKSVL
Sbjct: 267 NYFVAEDYSSLEGPSMNELYESGGLKSTLDGKVMLLAGTTVGGGSAINWSASIRTPKSVL 326

Query: 181 DEWSTDRKIPFFGSFEYQSAMDAVCDRIGVTYGCSIESFQNQVFRKGCENLGLKVDSVPR 240
           DEWSTD KIP +GS +YQ AMDAVCDRIGVT GC +ESFQNQV RKGCENLGLKVDSV R
Sbjct: 327 DEWSTDHKIPLYGSIDYQLAMDAVCDRIGVTRGCRVESFQNQVLRKGCENLGLKVDSVSR 386

Query: 241 NSSVNHYCGSCSYGCRVGDKKGTDSTWLVDAVESGAVILTGCKADKLILENTNSTNRNKK 300
           NSS +HYCGSC YGCRVGDKKGTD+TWLVDAV+ GAVILTGC ADK ILENTN+T++  K
Sbjct: 387 NSSEDHYCGSCCYGCRVGDKKGTDTTWLVDAVKCGAVILTGCHADKFILENTNNTSKKXK 446

Query: 301 CSGVVATLLSKTVTKKLHIEAKATISACGSLLTPPLLIASGLNNKNIGRNLHLHPVTLAW 360
           C GV+AT LSK VTKKLHIEAKATISACGSLLTPPLLIASGL NKNIG NLHLHPV+L W
Sbjct: 447 CIGVIATPLSKNVTKKLHIEAKATISACGSLLTPPLLIASGLRNKNIGTNLHLHPVSLVW 506

Query: 361 GYFPERLSDFTGKIHEGGLITSIHKVVSEDSNSHAIIETPALGPASFAALFPWTSGRDMK 420
           GYFPE LSDF GKI+EGGLIT +H VVS+ SNSHAIIETPA+GPASFAALFPWTSGR+MK
Sbjct: 507 GYFPEELSDFKGKIYEGGLITCLHNVVSKYSNSHAIIETPAVGPASFAALFPWTSGREMK 566

Query: 421 DAMAKYARTSHLFALVRDRGAGEVKVNADVKYRLDQTDKENLKIGLRQALRILIAAGATE 480
           DAM KY+RT+HLFALVRD+GAGE+KV A+VKYRL+QTDKENLKIGLRQALRILIAAGATE
Sbjct: 567 DAMVKYSRTAHLFALVRDQGAGEIKVKANVKYRLNQTDKENLKIGLRQALRILIAAGATE 626

Query: 481 VGTYRSDGQRIRCEGANKEDIEEFLDTVEAVGGPSSREEHWTMYCSAHQMSSCRMGSTEE 540
           +GTYRSDGQRIRC+G  KED+EEFLD+VEAVGGP SR+++WTMYCSAHQMSSCRMGST+E
Sbjct: 627 IGTYRSDGQRIRCQGTKKEDLEEFLDSVEAVGGPWSRQKYWTMYCSAHQMSSCRMGSTKE 686

Query: 541 EGAVDENGESWEAEGLFVCDGSVLPTAVGVNPMITIQSTAYCISNRIAHSFN 593
           EGAVDENGESWEAEGLFVCDGSVLPTAVGVNPMITIQSTAYCIS +IA S N
Sbjct: 687 EGAVDENGESWEAEGLFVCDGSVLPTAVGVNPMITIQSTAYCISKKIAESLN 738

BLAST of Sed0012772 vs. ExPASy TrEMBL
Match: A0A0A0LBV7 (Long-chain-alcohol oxidase OS=Cucumis sativus OX=3659 GN=Csa_3G575330 PE=3 SV=1)

HSP 1 Score: 1022.7 bits (2643), Expect = 6.2e-295
Identity = 492/592 (83.11%), Postives = 548/592 (92.57%), Query Frame = 0

Query: 1   MAFMVIKVFCCFRFFSRVDEESKNVAWEAMGYRVDTQKRLNNTRNERPLERGVVETGKED 60
           M FM+IK+FCCF+FFSR+DE +KNVAW+ MGY+VDT+K+LN+T+ ERPLERG++ETG ED
Sbjct: 147 MTFMIIKIFCCFKFFSRIDEGTKNVAWKGMGYKVDTRKQLNHTQKERPLERGMIETGNED 206

Query: 61  DSTFLKSLSLKGLEATEDLRHGNAYKIKCDVAIVGSGCGGGVAAAVLARSGLKVVVLEKG 120
           DST L+SLS KGLE  ED +HGNAYKIKCDV IVGSGCGGGVAAAVLA+SGLKVVVLEKG
Sbjct: 207 DSTLLRSLSQKGLEVIEDKKHGNAYKIKCDVVIVGSGCGGGVAAAVLAKSGLKVVVLEKG 266

Query: 121 NYFVPEDYSSLEGPSMNELYELGGLKSTVDGKVMLLAGSTVGGGSAINWSASIRTPKSVL 180
           NYFV EDYSSLEGPSMNELYE GGLKST+DGKVMLLAG+TVGGGSAINWSASI+TPKS+L
Sbjct: 267 NYFVAEDYSSLEGPSMNELYESGGLKSTLDGKVMLLAGTTVGGGSAINWSASIKTPKSIL 326

Query: 181 DEWSTDRKIPFFGSFEYQSAMDAVCDRIGVTYGCSIESFQNQVFRKGCENLGLKVDSVPR 240
           DEWSTD KIP +GS +YQ AMDAVCDRIGVT  C +ESFQNQV RKGCENLGLKVDSV R
Sbjct: 327 DEWSTDYKIPLYGSIDYQLAMDAVCDRIGVTEECPVESFQNQVLRKGCENLGLKVDSVSR 386

Query: 241 NSSVNHYCGSCSYGCRVGDKKGTDSTWLVDAVESGAVILTGCKADKLILENTNSTNRNKK 300
           NSS +HYCGSC YGCRVGDKKGTD+TWLVDAVE GAVILTGC+ADK IL+NTN+T++ K+
Sbjct: 387 NSSKDHYCGSCCYGCRVGDKKGTDTTWLVDAVEYGAVILTGCQADKFILDNTNNTSKRKR 446

Query: 301 CSGVVATLLSKTVTKKLHIEAKATISACGSLLTPPLLIASGLNNKNIGRNLHLHPVTLAW 360
           C GV+AT  SK +TKKLHIEAKATISACGSLLTPPLLIASGL NKNIG NLHLHPV+L W
Sbjct: 447 CIGVIATPRSKNITKKLHIEAKATISACGSLLTPPLLIASGLRNKNIGTNLHLHPVSLVW 506

Query: 361 GYFPERLSDFTGKIHEGGLITSIHKVVSEDSNSHAIIETPALGPASFAALFPWTSGRDMK 420
           GYFPE LSDF GKI+EGGLITS+HKVVS+ SNSHAIIETPA+GPASFAALFPWTSGR+MK
Sbjct: 507 GYFPEELSDFKGKIYEGGLITSLHKVVSKYSNSHAIIETPAVGPASFAALFPWTSGREMK 566

Query: 421 DAMAKYARTSHLFALVRDRGAGEVKVNADVKYRLDQTDKENLKIGLRQALRILIAAGATE 480
           DAM KY+RT+HLFAL+RD+GAGE+KV A+VKYRL+QTDKENL+IGLRQALRILIAAGATE
Sbjct: 567 DAMVKYSRTAHLFALIRDQGAGEIKVKANVKYRLNQTDKENLRIGLRQALRILIAAGATE 626

Query: 481 VGTYRSDGQRIRCEGANKEDIEEFLDTVEAVGGPSSREEHWTMYCSAHQMSSCRMGSTEE 540
           VGTYRSDGQRIRC+G NKE++EEFLD+VEAVGGP SR+++WTMYCSAHQMSSCRMGST+E
Sbjct: 627 VGTYRSDGQRIRCQGTNKEELEEFLDSVEAVGGPLSRQKYWTMYCSAHQMSSCRMGSTKE 686

Query: 541 EGAVDENGESWEAEGLFVCDGSVLPTAVGVNPMITIQSTAYCISNRIAHSFN 593
           EGAVDENGESWEAEGLFVCDGSVLPTAVGVNPMITIQSTAYCIS +IA S N
Sbjct: 687 EGAVDENGESWEAEGLFVCDGSVLPTAVGVNPMITIQSTAYCISKKIAESLN 738

BLAST of Sed0012772 vs. TAIR 10
Match: AT3G23410.1 (fatty alcohol oxidase 3 )

HSP 1 Score: 748.4 bits (1931), Expect = 4.3e-216
Identity = 371/595 (62.35%), Postives = 456/595 (76.64%), Query Frame = 0

Query: 2   AFMVIKVFCCFRFFSRVDEESKNVAWEAMGYRVD-TQKRLNNTRNERPLERGVVETGKED 61
           AF+ IKV   F FFSRV+   +N AWEA+GYRV+  + + + T NERPLE+G+VET +E 
Sbjct: 146 AFVYIKVAFLFCFFSRVNPNGENPAWEAIGYRVNPDENKPSETHNERPLEKGIVETMEET 205

Query: 62  DSTFLKSLSLKGLEATEDLRHGNAYKIKCDVAIVGSGCGGGVAAAVLARSGLKVVVLEKG 121
           + T L+SL+ KGLEA  D  H +A +IKCDV +VGSG GGGVAA+VLA+SGLKVVVLEKG
Sbjct: 206 EQTLLESLAHKGLEAVLDTEH-DAIRIKCDVVVVGSGSGGGVAASVLAKSGLKVVVLEKG 265

Query: 122 NYFVPEDYSSLEGPSMNELYELGGLKSTVDGKVMLLAGSTVGGGSAINWSASIRTPKSVL 181
           +YF P ++   EGP +++LYE GG+  +VDG  M+LAG+TVGGGSA+NWSA I+TPKSVL
Sbjct: 266 SYFTPSEHRPFEGPGLDKLYENGGILPSVDGSFMVLAGATVGGGSAVNWSACIKTPKSVL 325

Query: 182 DEWSTDRKIPFFGSFEYQSAMDAVCDRIGVTYGCSIESFQNQVFRKGCENLGLKVDSVPR 241
            EWS D+ IP FG+ EY +AM+ V  R+GVT  C +ESFQNQ+ RKGCENLG  V++VPR
Sbjct: 326 QEWSEDQNIPLFGTKEYLTAMEVVWKRMGVTEKCELESFQNQILRKGCENLGFNVENVPR 385

Query: 242 NSSVNHYCGSCSYGCRVGDKKGTDSTWLVDAVESGAVILTGCKADKLILENTNSTNRNK- 301
           NSS +HYCGSC YGCR GDKKG+D TWLVDAV  GAVILTGCKA++ ILE   S    K 
Sbjct: 386 NSSESHYCGSCGYGCRQGDKKGSDRTWLVDAVGHGAVILTGCKAERFILEKNGSNKGGKQ 445

Query: 302 -KCSGVVATLLSKTVTKKLHIEAKATISACGSLLTPPLLIASGLNNKNIGRNLHLHPVTL 361
            KC GV+A  L+  + K L IEAK T+SA G+LLTPPL+I+SGL N+NIG+NLHLHPV +
Sbjct: 446 MKCLGVMAKSLNGNIAKMLKIEAKVTVSAGGALLTPPLMISSGLRNRNIGKNLHLHPVLM 505

Query: 362 AWGYFPERLS---DFTGKIHEGGLITSIHKVVSEDSNSHAIIETPALGPASFAALFPWTS 421
           AWGYFP++ S    F G  +EGG+ITS+ KV+SEDS   AIIETP LGP SF+ L PWTS
Sbjct: 506 AWGYFPDKESSNISFKGNSYEGGIITSVSKVLSEDSEVRAIIETPQLGPGSFSVLTPWTS 565

Query: 422 GRDMKDAMAKYARTSHLFALVRDRGAGEVKVNADVKYRLDQTDKENLKIGLRQALRILIA 481
           G DMK  MA+Y+RT+ L  +VRDRG+GEVK    + Y +D+TD++NLK GLR++LRILIA
Sbjct: 566 GLDMKKRMARYSRTASLITIVRDRGSGEVKTEGRINYTVDKTDRDNLKAGLRESLRILIA 625

Query: 482 AGATEVGTYRSDGQRIRCEGANKEDIEEFLDTVEAVGGPSSREEHWTMYCSAHQMSSCRM 541
           AGA EVGT+RSDGQR+ C+G N+  I+EFLD+V    G     E W +Y SAHQM SCR+
Sbjct: 626 AGAEEVGTHRSDGQRLICKGVNENSIQEFLDSVSTEEGAKGMTEKWNVYSSAHQMGSCRI 685

Query: 542 GSTEEEGAVDENGESWEAEGLFVCDGSVLPTAVGVNPMITIQSTAYCISNRIAHS 591
           G  E+EGA+D NGESWEAE LFVCD S LP+AVGVNPMIT+ STAYCIS RIA S
Sbjct: 686 GENEKEGAIDLNGESWEAEKLFVCDASALPSAVGVNPMITVMSTAYCISTRIAKS 739

BLAST of Sed0012772 vs. TAIR 10
Match: AT4G28570.1 (Long-chain fatty alcohol dehydrogenase family protein )

HSP 1 Score: 743.0 bits (1917), Expect = 1.8e-214
Identity = 372/597 (62.31%), Postives = 454/597 (76.05%), Query Frame = 0

Query: 1   MAFMVIKVFCCFRFFSRVDEESKNVAWEAMGYRVDTQKRLNNTRNE-----RPLERGVVE 60
           + F + K +  F FFS+ DE  KN A EA+GY +D  +R +N ++E     RPLE+G++E
Sbjct: 146 ITFFLAKFYTLFYFFSQTDENLKNPALEAIGYCIDGTERSSNKKSEADEKRRPLEKGIIE 205

Query: 61  TGKEDDSTFLKSLSLKGLEATEDLRHGNAYKIKCDVAIVGSGCGGGVAAAVLARSGLKVV 120
           T  E D T  +SL+ KG+    D    N Y+I+CD  +VGSG GGGVAAA LA++GLKV+
Sbjct: 206 TMHESDVTITQSLTEKGVHVARD-DGDNVYRIRCDAVVVGSGSGGGVAAANLAKAGLKVL 265

Query: 121 VLEKGNYFVPEDYSSLEGPSMNELYELGGLKSTVDGKVMLLAGSTVGGGSAINWSASIRT 180
           VLEKGNYF   DYS LE PSM ELYE GGL +TVDGK MLLAGS VGGG+A+NWSASIRT
Sbjct: 266 VLEKGNYFTAHDYSGLEVPSMLELYEKGGLLTTVDGKFMLLAGSAVGGGTAVNWSASIRT 325

Query: 181 PKSVLDEWSTDRKIPFFGSFEYQSAMDAVCDRIGVTYGCSIESFQNQVFRKGCENLGLKV 240
           P  VL EWS   KI FFGS EYQSAMD V  RIGVT  C    FQNQV RKGCE LGL+V
Sbjct: 326 PDHVLQEWSEGSKIKFFGSQEYQSAMDEVTIRIGVTERCVKHGFQNQVLRKGCERLGLQV 385

Query: 241 DSVPRNSSVNHYCGSCSYGCRVGDKKGTDSTWLVDAVESGAVILTGCKADKLIL-ENTNS 300
           +SVPRNS  +HYCG C YGCR G K GTD TWLVDAVE+GAVILTG KA++ +L +NT+S
Sbjct: 386 ESVPRNSPEDHYCGLCGYGCRAGAKNGTDQTWLVDAVENGAVILTGIKAERFVLVDNTSS 445

Query: 301 TN-RNKKCSGVVATLLSKTVTKKLHIEAKATISACGSLLTPPLLIASGLNNKNIGRNLHL 360
           +N R K+C GV A+ +   + KK  IEA+ T+S+ GSLLTPPL+++SGL N NIGRNL L
Sbjct: 446 SNERKKRCVGVFASSVGGKIGKKFIIEARVTVSSAGSLLTPPLMLSSGLKNPNIGRNLKL 505

Query: 361 HPVTLAWGYFPERLSDFTGKIHEGGLITSIHKVVSEDSNSHAIIETPALGPASFAALFPW 420
           HPV + WGYFPE+ S+F+GK++EGG+ITS+H +   +S   AI+E P +GPAS+A L PW
Sbjct: 506 HPVLMTWGYFPEKDSEFSGKMYEGGIITSVHHMNDTESGCKAILENPLIGPASYAGLSPW 565

Query: 421 TSGRDMKDAMAKYARTSHLFALVRDRGAGEVKVNADVKYRLDQTDKENLKIGLRQALRIL 480
            SG D+K+ M KY RT+HLFALVRD G+GEV +  +V YR  + D+ENL+ GLRQALR+ 
Sbjct: 566 VSGPDLKERMIKYGRTAHLFALVRDLGSGEVMMENEVTYRTTKKDRENLRAGLRQALRVS 625

Query: 481 IAAGATEVGTYRSDGQRIRCEGANKEDIEEFLDTVEAVGGPSSREEHWTMYCSAHQMSSC 540
           +AAGA EVGTYRSDGQ+++CE   KE +EEFLD V+AVGG  ++ E+WT Y SAHQM SC
Sbjct: 626 VAAGAVEVGTYRSDGQKMKCEAITKEAMEEFLDEVDAVGGVGTKGEYWTTYFSAHQMGSC 685

Query: 541 RMGSTEEEGAVDENGESWEAEGLFVCDGSVLPTAVGVNPMITIQSTAYCISNRIAHS 591
           RMG T EEGA+DENGESWEAEGLFVCDGS+LP+AVGVNPMITIQSTAYCIS++I  S
Sbjct: 686 RMGVTAEEGALDENGESWEAEGLFVCDGSILPSAVGVNPMITIQSTAYCISSKIVDS 741

BLAST of Sed0012772 vs. TAIR 10
Match: AT1G03990.1 (Long-chain fatty alcohol dehydrogenase family protein )

HSP 1 Score: 709.5 bits (1830), Expect = 2.2e-204
Identity = 351/599 (58.60%), Postives = 444/599 (74.12%), Query Frame = 0

Query: 1   MAFMVIKVFCCFRFFSRVDEESKNVAWEAMGYRVDTQKRLNNTRNERPLERGVVETGKED 60
           + FM+IK    F +F+  +E S+N  W+A+ Y V+  +  +  + ERPL+ G++ET KED
Sbjct: 159 IGFMMIKAIFLFYYFTWTNENSENPVWDAINYSVEIGENEDMEQKERPLDEGIIETAKED 218

Query: 61  DSTFLKSLSLKGLEATEDLRHGNAYKIKCDVAIVGSGCGGGVAAAVLARSGLKVVVLEKG 120
           + T  + +  KGL+ TED R  + YKI+CD  +VGSGCGGGVAAA+LA+SGL+VVV+EKG
Sbjct: 219 EMTIKQRMINKGLKVTED-RERDTYKIECDAVVVGSGCGGGVAAAILAKSGLRVVVIEKG 278

Query: 121 NYFVPEDYSSLEGPSMNELYELGGLKSTVDGKVMLLAGSTVGGGSAINWSASIRTPKSVL 180
           NYF P DYS+LEGPSM EL+E   L  T DG+   +AGSTVGGGS +NW+AS++TP +++
Sbjct: 279 NYFAPRDYSALEGPSMFELFESNSLMMTHDGRFRFMAGSTVGGGSVVNWAASLKTPDAII 338

Query: 181 DEWSTDRKIPFFGSFEYQSAMDAVCDRIGVTYGCSIESFQNQVFRKGCENLGLKVDSVPR 240
           +EWS  R I  + S +Y++AM  VC R+GVT     E FQNQ+ RKGCE LGL V  VPR
Sbjct: 339 EEWSVHRGISIYSSEKYKAAMGIVCKRLGVTEKIIREGFQNQILRKGCEKLGLDVTIVPR 398

Query: 241 NSSVNHYCGSCSYGCRVGDKKGTDSTWLVDAVESGAVILTGCKADKLILENTNS-----T 300
           NS+  HYCGSCSYGC  G+K+GTDSTWLVDAV + AVILT CKA+KLIL + ++     +
Sbjct: 399 NSTEKHYCGSCSYGCPTGEKRGTDSTWLVDAVNNNAVILTQCKAEKLILADNDANKREES 458

Query: 301 NRNKKCSGVVATLLSKTVTKKLHIEAKATISACGSLLTPPLLIASGLNNKNIGRNLHLHP 360
            R K+C GV A+L  +T  KKL I AK TI ACGSL TP LL +SGL N NI R LH+HP
Sbjct: 459 GRRKRCLGVAASLSHQT-RKKLQINAKVTIVACGSLKTPGLLASSGLKNSNISRGLHIHP 518

Query: 361 VTLAWGYFPERLSDFTGKIHEGGLITSIHKVVSEDSNSHAI-IETPALGPASFAALFPWT 420
           + +AWGYFPE+ S+  G  HEG ++TS+H V   DS +  I +ETPA+GP +FAAL PW 
Sbjct: 519 IMMAWGYFPEKNSELEGAAHEGEIVTSLHYVHPMDSTTPNITLETPAIGPGTFAALTPWV 578

Query: 421 SGRDMKDAMAKYARTSHLFALVRDRGAGEVKVNADVKYRLDQTDKENLKIGLRQALRILI 480
           SG DMK+ MAKYART+H+FA+VRD G GEVK +  VKYRL + D+ENL IGL+QALRIL+
Sbjct: 579 SGSDMKERMAKYARTAHIFAMVRDEGVGEVKGDI-VKYRLTKADEENLTIGLKQALRILV 638

Query: 481 AAGATEVGTYRSDGQRIRCEGANKEDIEEFLDTVEAVGGPSSREEHWTMYCSAHQMSSCR 540
           AAGA EVGTYRSDGQR++C+G  ++D+E FLDTV A  G  S  +HWT   +AHQ+  CR
Sbjct: 639 AAGAAEVGTYRSDGQRMKCDGIKQKDLEAFLDTVNAPPGVVSMSKHWTQSFTAHQIGCCR 698

Query: 541 MGSTEEEGAVDENGESWEAEGLFVCDGSVLPTAVGVNPMITIQSTAYCISNRIAHSFNK 594
           MG+TE+EGA+D  GESWEAE L+VCD SVLPTA+GVNPMIT+QSTAYCISNRIA    K
Sbjct: 699 MGATEKEGAIDGKGESWEAEDLYVCDASVLPTALGVNPMITVQSTAYCISNRIAELMKK 754

BLAST of Sed0012772 vs. TAIR 10
Match: AT4G19380.1 (Long-chain fatty alcohol dehydrogenase family protein )

HSP 1 Score: 508.8 bits (1309), Expect = 5.7e-144
Identity = 276/619 (44.59%), Postives = 381/619 (61.55%), Query Frame = 0

Query: 1   MAFMVIKVFCCFRFFSRVDEESKNVAWEAMGY------RVDTQKRLNNTRNER------- 60
           M F  IK+     FF++VDE+ +N+AW+A+GY        D +  LN  + ++       
Sbjct: 117 MLFRTIKLISALVFFTQVDEKGRNLAWKAIGYNGPSPDHSDHEVELNEEKKKKKPEEIFG 176

Query: 61  PLERGVVETGKEDDSTFLKSLSLKGLEATEDLRHGN-------AYKIKCDVAIVGSGCGG 120
           PL  G+V+  K       K L+ +G   +   R+ N         KI+CD  +VGSG GG
Sbjct: 177 PLYNGIVDL-KSPREAVEKKLAGRGFAVSNQKRNTNGSSISDPVMKIQCDAVVVGSGSGG 236

Query: 121 GVAAAVLARSGLKVVVLEKGNYFVPEDYSSLEGPSMNELYELGGLKSTVDGKVMLLAGST 180
           GVAA VLA++G KV+V+E GNY+     S LEG +M+++Y  GGL +T D  V++LAGST
Sbjct: 237 GVAAGVLAKAGYKVLVIESGNYYARSKLSLLEGQAMDDMYLSGGLLATSDTNVVILAGST 296

Query: 181 VGGGSAINWSASIRTPKSVLDEWSTDRKIPFFGSFEYQSAMDAVCDRIGVTYGCSIESFQ 240
           VGGGS INWSASI+TP+ V+ EW+   K+  FGS  Y+ AMD VC R+GV  G   E F 
Sbjct: 297 VGGGSTINWSASIKTPEHVMKEWAEKSKLEMFGSDLYREAMDVVCKRMGVQCGFVEEGFN 356

Query: 241 NQVFRKGCENLGLKVDSVPRNSSVNHYCGSCSYGCRVGDKKGTDSTWLVDAVES-GAVIL 300
           N+V RKGCE LGL V ++PRN+  +HYCG C  GC+ G K+GT  TWLVD VES   +IL
Sbjct: 357 NEVLRKGCEKLGLPVKNIPRNAPSDHYCGFCCLGCKKGQKQGTSETWLVDLVESDNGLIL 416

Query: 301 TGCKADKLILENTNSTNRNKKCSGVVATLLSKTVTKKLHIEAKATISACGSLLTPPLLIA 360
            GC+A +++ +      + KK +GV      +       +E++ TI ACG+L TP LL  
Sbjct: 417 PGCQATEVMYD--CEQGKKKKATGVAFAFGEEIYV----VESRVTIVACGALRTPHLLKR 476

Query: 361 SGLNNKNIGRNLHLHPVTLAWGYFPE--RLSDFTGKIHEGGLITSIHKVVSEDSNS---H 420
           SGL N NIGRNL LHPV +AWG+FPE  +  +   K +EGG++T++  VV E+++S    
Sbjct: 477 SGLKNSNIGRNLCLHPVVMAWGWFPEEDKWPEKKKKSYEGGIMTAMSSVVIEETHSSYGE 536

Query: 421 AIIETPALGPASFAALFPWTSGRDMKDAMAKYARTSHLFALVRDRGAGEVKVNADVKYRL 480
            +I+TPAL P  F+ + PWTS +D K  M K++RT+H+FAL+RD+G G +     + Y L
Sbjct: 537 MVIQTPALHPGMFSGIIPWTSSKDFKTRMLKFSRTAHIFALLRDKGTGTIDSKTYIDYNL 596

Query: 481 DQTDKENLKIGLRQALRILIAAGATEVGTYRSDGQRIRCEGANKEDIEEFLDTVEAVGGP 540
           +  D+E+LK GL + L+IL AAGA E+GT+ S+G+ +    A+  +IE F+    +    
Sbjct: 597 NDEDEESLKNGLERVLKILAAAGAEEIGTHHSEGRSLNVRTASSLEIERFVREESS---- 656

Query: 541 SSREEHWTMYCSAHQMSSCRMGSTEEEGAVDENGESWEAEGLFVCDGSVLPTAVGVNPMI 594
              ++     CSAHQM SCRMG   EE AV   GE+WE E LFV D SV PTA+GVNPM+
Sbjct: 657 KPLKDLSGQICSAHQMGSCRMGIRPEESAVRPTGETWEVERLFVADTSVFPTALGVNPMV 716

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038888257.11.4e-30185.81long-chain-alcohol oxidase FAO2-like [Benincasa hispida][more]
XP_022967423.14.1e-30185.14long-chain-alcohol oxidase FAO2-like [Cucurbita maxima] >XP_022967424.1 long-cha... [more]
XP_022963812.13.5e-30084.80long-chain-alcohol oxidase FAO2 [Cucurbita moschata] >XP_022963813.1 long-chain-... [more]
KAG7011279.13.5e-30084.92Long-chain-alcohol oxidase FAO2, partial [Cucurbita argyrosperma subsp. argyrosp... [more]
XP_023553773.17.2e-29884.86long-chain-alcohol oxidase FAO2-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
B5WWZ92.1e-22865.55Long-chain-alcohol oxidase FAO2 OS=Lotus japonicus OX=34305 GN=FAO2 PE=2 SV=1[more]
B5WWZ85.9e-21862.84Long-chain-alcohol oxidase FAO1 OS=Lotus japonicus OX=34305 GN=FAO1 PE=1 SV=1[more]
Q9LW566.1e-21562.35Long-chain-alcohol oxidase FAO3 OS=Arabidopsis thaliana OX=3702 GN=FAO3 PE=1 SV=... [more]
Q94BP32.5e-21362.31Long-chain-alcohol oxidase FAO4B OS=Arabidopsis thaliana OX=3702 GN=FAO4B PE=2 S... [more]
Q9ZWB93.1e-20358.60Long-chain-alcohol oxidase FAO1 OS=Arabidopsis thaliana OX=3702 GN=FAO1 PE=3 SV=... [more]
Match NameE-valueIdentityDescription
A0A6J1HWN72.0e-30185.14Long-chain-alcohol oxidase OS=Cucurbita maxima OX=3661 GN=LOC111466966 PE=3 SV=1[more]
A0A6J1HG831.7e-30084.80Long-chain-alcohol oxidase OS=Cucurbita moschata OX=3662 GN=LOC111464000 PE=3 SV... [more]
A0A5A7T3R34.3e-29683.95Long-chain-alcohol oxidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaff... [more]
A0A1S3BHB12.1e-29583.78Long-chain-alcohol oxidase OS=Cucumis melo OX=3656 GN=LOC103489850 PE=3 SV=1[more]
A0A0A0LBV76.2e-29583.11Long-chain-alcohol oxidase OS=Cucumis sativus OX=3659 GN=Csa_3G575330 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT3G23410.14.3e-21662.35fatty alcohol oxidase 3 [more]
AT4G28570.11.8e-21462.31Long-chain fatty alcohol dehydrogenase family protein [more]
AT1G03990.12.2e-20458.60Long-chain fatty alcohol dehydrogenase family protein [more]
AT4G19380.15.7e-14444.59Long-chain fatty alcohol dehydrogenase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Chayote (edule) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000172Glucose-methanol-choline oxidoreductase, N-terminalPFAMPF00732GMC_oxred_Ncoord: 99..356
e-value: 6.9E-65
score: 219.3
IPR012400Long-chain-alcohol oxidasePIRSFPIRSF028937Lg_Ch_AOcoord: 1..593
e-value: 2.3E-281
score: 933.3
IPR007867Glucose-methanol-choline oxidoreductase, C-terminalPFAMPF05199GMC_oxred_Ccoord: 447..582
e-value: 4.4E-24
score: 85.6
IPR036188FAD/NAD(P)-binding domain superfamilyGENE3D3.50.50.60coord: 420..590
e-value: 1.9E-15
score: 59.0
IPR036188FAD/NAD(P)-binding domain superfamilyGENE3D3.50.50.60coord: 78..396
e-value: 2.4E-27
score: 97.8
IPR036188FAD/NAD(P)-binding domain superfamilySUPERFAMILY51905FAD/NAD(P)-binding domaincoord: 87..590
NoneNo IPR availablePANTHERPTHR46056LONG-CHAIN-ALCOHOL OXIDASEcoord: 2..593
NoneNo IPR availablePANTHERPTHR46056:SF12LONG-CHAIN-ALCOHOL OXIDASE FAO4Bcoord: 2..593

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sed0012772.1Sed0012772.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0050660 flavin adenine dinucleotide binding
molecular_function GO:0046577 long-chain-alcohol oxidase activity
molecular_function GO:0046593 mandelonitrile lyase activity
molecular_function GO:0016614 oxidoreductase activity, acting on CH-OH group of donors