Sed0012335 (gene) Chayote v1

Overview
NameSed0012335
Typegene
OrganismSechium edule (Chayote v1)
Descriptionalkane hydroxylase MAH1-like
LocationLG05: 12190102 .. 12191647 (-)
RNA-Seq ExpressionSed0012335
SyntenySed0012335
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAATGTACTTTGTTTGCAGACCTCCCATGGCGTTCTCCACCCTTCTCCCTTTCTTACCCTTCATTACTTTCTCCATTTTCTTTGTTTTTCTCAAATGGAACCCTCGTGGAATTCCCTGGAATTGGCCCTTTCTGGGAATGCTCCCTACCGTTGCTCTCAATATCCATCGAATTCACGACCGTGTTACTGAAATCTTAAACCAAGCTGGCTCCACTTTCTTCTTCAAAGGCCTTTGGTTTTCCGGTCTAGACTTTTTCTTAACCGCGGACCCTTCCAATATACACCACATTTTGAGTACCAATTTCGAACGATATCCGAAAGGGCCTGATTTCAAGTACATCTTTGACGTACTCGGAGATGGGATTTTCAACTCCGACTCTGTTTCGTGGCAGAGCCAACGCAGAACGGCTCATGCTCTGCTTCGTACCCATAGCTTTCTTCAATTCTCGGAAGAAATTACGATCAAGAAAGTCAAGCAAGGGTTGATTCCAATACTTGAAAGTGTCTCTGAACATGGGTCGGTGTTGGATTTGCAAGATCTGTTCCAGAGATTCTCGTTTGATTCAACTTGTATGCTGGTCACTGGCTTCGACCTCTGTAGTTTATCTCTCGAATTCCCTGAAATTCCATTCTCAAAGGCTATGGACGATGTGGAGGAAGTGATTTTCCTCCGCCATTTCTTCCCTAAGAAGCTGTGGGAGTTTCAAAGCAAGTTCCAAATTGGACAACCCAAGAGACTAAAACAAGCTTGGGAAATCATAGATCAAACAATTGCCAAACTTCTAGCTTCGAAAAGAGAGAGCTTGAAGATGATGATGATCATGAAAGAAGAATCAGACATGATCACATCCTACCTCACAGATTATAAACACGACGACGACAAGTTCTTAAGAGACACTATTTTAAATATCACGATTGCTGGTCGGGACACTCTCAGCTCAGCTCTATCTTGGTTTTTCTTTTGCCTCTCAAATAATCCAACCGTAGAAGCAAAGATAAGAGAAGAGCTCAAAACAGCCATCCCAACAAACGAAGGTCAAAATCAGCAATGGATATTTCCAATTGAAGAGGTGAAGAAGCTCGTATATTTTCATGGAGCTTTATGCGAAACACTTAGACTTTATCCACCGGTTCCATTCCAACACAAAGTCCCTTCCCAAACTGACATTCTTCCATCGGGTCATTATGTCAATCCTAAAACAAAGATATTGTTTTCATTACACGCGTTGGGAAGGATGAGCGATGTTTGGGGAAATGATTGCATGGATTTCAAGCCTGAAAGATGGATTTCAGAAAGGGGAAGTATCAAACATGTGCCATCTTACAAATTCTTGGCCTTCAATGCGGGGCCAAGGACTTGTTTGGGAAAGGAAGTGGCTTTTCTTGAGATGAAGATAGTTGCAGCTGCTATTATTCATAACTACAATGTTGAATTAATAGGACATAAGGTTGTTCCAAGTGCTTCTATTATTCTTCACATGAAGCATGGATTCAAAATCAAGATTTCAAGAAGGTGGCCATCTTCAAAACTCAATTAGTCCGTCT

mRNA sequence

AAAATGTACTTTGTTTGCAGACCTCCCATGGCGTTCTCCACCCTTCTCCCTTTCTTACCCTTCATTACTTTCTCCATTTTCTTTGTTTTTCTCAAATGGAACCCTCGTGGAATTCCCTGGAATTGGCCCTTTCTGGGAATGCTCCCTACCGTTGCTCTCAATATCCATCGAATTCACGACCGTGTTACTGAAATCTTAAACCAAGCTGGCTCCACTTTCTTCTTCAAAGGCCTTTGGTTTTCCGGTCTAGACTTTTTCTTAACCGCGGACCCTTCCAATATACACCACATTTTGAGTACCAATTTCGAACGATATCCGAAAGGGCCTGATTTCAAGTACATCTTTGACGTACTCGGAGATGGGATTTTCAACTCCGACTCTGTTTCGTGGCAGAGCCAACGCAGAACGGCTCATGCTCTGCTTCGTACCCATAGCTTTCTTCAATTCTCGGAAGAAATTACGATCAAGAAAGTCAAGCAAGGGTTGATTCCAATACTTGAAAGTGTCTCTGAACATGGGTCGGTGTTGGATTTGCAAGATCTGTTCCAGAGATTCTCGTTTGATTCAACTTGTATGCTGGTCACTGGCTTCGACCTCTGTAGTTTATCTCTCGAATTCCCTGAAATTCCATTCTCAAAGGCTATGGACGATGTGGAGGAAGTGATTTTCCTCCGCCATTTCTTCCCTAAGAAGCTGTGGGAGTTTCAAAGCAAGTTCCAAATTGGACAACCCAAGAGACTAAAACAAGCTTGGGAAATCATAGATCAAACAATTGCCAAACTTCTAGCTTCGAAAAGAGAGAGCTTGAAGATGATGATGATCATGAAAGAAGAATCAGACATGATCACATCCTACCTCACAGATTATAAACACGACGACGACAAGTTCTTAAGAGACACTATTTTAAATATCACGATTGCTGGTCGGGACACTCTCAGCTCAGCTCTATCTTGGTTTTTCTTTTGCCTCTCAAATAATCCAACCGTAGAAGCAAAGATAAGAGAAGAGCTCAAAACAGCCATCCCAACAAACGAAGGTCAAAATCAGCAATGGATATTTCCAATTGAAGAGGTGAAGAAGCTCGTATATTTTCATGGAGCTTTATGCGAAACACTTAGACTTTATCCACCGGTTCCATTCCAACACAAAGTCCCTTCCCAAACTGACATTCTTCCATCGGGTCATTATGTCAATCCTAAAACAAAGATATTGTTTTCATTACACGCGTTGGGAAGGATGAGCGATGTTTGGGGAAATGATTGCATGGATTTCAAGCCTGAAAGATGGATTTCAGAAAGGGGAAGTATCAAACATGTGCCATCTTACAAATTCTTGGCCTTCAATGCGGGGCCAAGGACTTGTTTGGGAAAGGAAGTGGCTTTTCTTGAGATGAAGATAGTTGCAGCTGCTATTATTCATAACTACAATGTTGAATTAATAGGACATAAGGTTGTTCCAAGTGCTTCTATTATTCTTCACATGAAGCATGGATTCAAAATCAAGATTTCAAGAAGGTGGCCATCTTCAAAACTCAATTAGTCCGTCT

Coding sequence (CDS)

ATGTACTTTGTTTGCAGACCTCCCATGGCGTTCTCCACCCTTCTCCCTTTCTTACCCTTCATTACTTTCTCCATTTTCTTTGTTTTTCTCAAATGGAACCCTCGTGGAATTCCCTGGAATTGGCCCTTTCTGGGAATGCTCCCTACCGTTGCTCTCAATATCCATCGAATTCACGACCGTGTTACTGAAATCTTAAACCAAGCTGGCTCCACTTTCTTCTTCAAAGGCCTTTGGTTTTCCGGTCTAGACTTTTTCTTAACCGCGGACCCTTCCAATATACACCACATTTTGAGTACCAATTTCGAACGATATCCGAAAGGGCCTGATTTCAAGTACATCTTTGACGTACTCGGAGATGGGATTTTCAACTCCGACTCTGTTTCGTGGCAGAGCCAACGCAGAACGGCTCATGCTCTGCTTCGTACCCATAGCTTTCTTCAATTCTCGGAAGAAATTACGATCAAGAAAGTCAAGCAAGGGTTGATTCCAATACTTGAAAGTGTCTCTGAACATGGGTCGGTGTTGGATTTGCAAGATCTGTTCCAGAGATTCTCGTTTGATTCAACTTGTATGCTGGTCACTGGCTTCGACCTCTGTAGTTTATCTCTCGAATTCCCTGAAATTCCATTCTCAAAGGCTATGGACGATGTGGAGGAAGTGATTTTCCTCCGCCATTTCTTCCCTAAGAAGCTGTGGGAGTTTCAAAGCAAGTTCCAAATTGGACAACCCAAGAGACTAAAACAAGCTTGGGAAATCATAGATCAAACAATTGCCAAACTTCTAGCTTCGAAAAGAGAGAGCTTGAAGATGATGATGATCATGAAAGAAGAATCAGACATGATCACATCCTACCTCACAGATTATAAACACGACGACGACAAGTTCTTAAGAGACACTATTTTAAATATCACGATTGCTGGTCGGGACACTCTCAGCTCAGCTCTATCTTGGTTTTTCTTTTGCCTCTCAAATAATCCAACCGTAGAAGCAAAGATAAGAGAAGAGCTCAAAACAGCCATCCCAACAAACGAAGGTCAAAATCAGCAATGGATATTTCCAATTGAAGAGGTGAAGAAGCTCGTATATTTTCATGGAGCTTTATGCGAAACACTTAGACTTTATCCACCGGTTCCATTCCAACACAAAGTCCCTTCCCAAACTGACATTCTTCCATCGGGTCATTATGTCAATCCTAAAACAAAGATATTGTTTTCATTACACGCGTTGGGAAGGATGAGCGATGTTTGGGGAAATGATTGCATGGATTTCAAGCCTGAAAGATGGATTTCAGAAAGGGGAAGTATCAAACATGTGCCATCTTACAAATTCTTGGCCTTCAATGCGGGGCCAAGGACTTGTTTGGGAAAGGAAGTGGCTTTTCTTGAGATGAAGATAGTTGCAGCTGCTATTATTCATAACTACAATGTTGAATTAATAGGACATAAGGTTGTTCCAAGTGCTTCTATTATTCTTCACATGAAGCATGGATTCAAAATCAAGATTTCAAGAAGGTGGCCATCTTCAAAACTCAATTAG

Protein sequence

MYFVCRPPMAFSTLLPFLPFITFSIFFVFLKWNPRGIPWNWPFLGMLPTVALNIHRIHDRVTEILNQAGSTFFFKGLWFSGLDFFLTADPSNIHHILSTNFERYPKGPDFKYIFDVLGDGIFNSDSVSWQSQRRTAHALLRTHSFLQFSEEITIKKVKQGLIPILESVSEHGSVLDLQDLFQRFSFDSTCMLVTGFDLCSLSLEFPEIPFSKAMDDVEEVIFLRHFFPKKLWEFQSKFQIGQPKRLKQAWEIIDQTIAKLLASKRESLKMMMIMKEESDMITSYLTDYKHDDDKFLRDTILNITIAGRDTLSSALSWFFFCLSNNPTVEAKIREELKTAIPTNEGQNQQWIFPIEEVKKLVYFHGALCETLRLYPPVPFQHKVPSQTDILPSGHYVNPKTKILFSLHALGRMSDVWGNDCMDFKPERWISERGSIKHVPSYKFLAFNAGPRTCLGKEVAFLEMKIVAAAIIHNYNVELIGHKVVPSASIILHMKHGFKIKISRRWPSSKLN
Homology
BLAST of Sed0012335 vs. NCBI nr
Match: XP_022950281.1 (alkane hydroxylase MAH1-like [Cucurbita moschata])

HSP 1 Score: 778.5 bits (2009), Expect = 3.6e-221
Identity = 373/508 (73.43%), Postives = 434/508 (85.43%), Query Frame = 0

Query: 5   CRPPMA----FSTLLPFLPFITFSIFFVFLKWNPRGIPWNWPFLGMLPTVALNIHRIHDR 64
           CR PMA     S LL F+PF+ F++F + LKWN  G+PWNWPF GM PT+  +IHR+H R
Sbjct: 30  CRAPMASLSFLSILLAFIPFV-FALFVLSLKWNFNGLPWNWPFFGMTPTILFHIHRVHHR 89

Query: 65  VTEILNQAGSTFFFKGLWFSGLDFFLTADPSNIHHILSTNFERYPKGPDFKYIFDVLGDG 124
           +T IL Q+ STF FKG WF+GLDFFLTADPSNIHHILSTNF+RYPKG DFKY+FDVLGDG
Sbjct: 90  LTRILQQSPSTFLFKGAWFAGLDFFLTADPSNIHHILSTNFDRYPKGTDFKYMFDVLGDG 149

Query: 125 IFNSDSVSWQSQRRTAHALLRTHSFLQFSEEITIKKVKQGLIPILESVSEHGSVLDLQDL 184
           I NSDSVSW++QR TAHAL+R   FL F E I +KKV++GL+ +LESV E+GSVLDLQDL
Sbjct: 150 ISNSDSVSWKTQRTTAHALVRQEKFLHFLERIAMKKVREGLVSVLESVCENGSVLDLQDL 209

Query: 185 FQRFSFDSTCMLVTGFDLCSLSLEFPEIPFSKAMDDVEEVIFLRHFFPKKLWEFQSKFQI 244
           FQRFSFDS+CMLVTG DL SLSLEFP++PFSKAMDD+EEVIFLRH  PKK+WEFQ K QI
Sbjct: 210 FQRFSFDSSCMLVTGLDLHSLSLEFPQVPFSKAMDDIEEVIFLRHLIPKKIWEFQKKLQI 269

Query: 245 GQPKRLKQAWEIIDQTIAKLLASKRESLKMMMIMKEES--DMITSYLTDYKHDDDKFLRD 304
           G PKRL++AWEIIDQTIA L+A+KRESL  +   K++   D+ITSY+ + K +DDK LRD
Sbjct: 270 GHPKRLQEAWEIIDQTIANLIATKRESLNQLREEKDQEGVDLITSYM-ENKENDDKMLRD 329

Query: 305 TILNITIAGRDTLSSALSWFFFCLSNNPTVEAKIREELKTAIPTNEGQNQQWIFPIEEVK 364
           T+LN  IAGRDTLSSALSWFFFCLSNNPTVEAKIREEL+T IPT+E + +  +F I+EV 
Sbjct: 330 TVLNFVIAGRDTLSSALSWFFFCLSNNPTVEAKIREELETTIPTSEPRGRWRMFSIKEVD 389

Query: 365 KLVYFHGALCETLRLYPPVPFQHKVPSQTDILPSGHYVNPKTKILFSLHALGRMSDVWGN 424
           KLVYFHGALCETLRLYPPVPFQHK+PSQ D+LPSGH+VNPKTKI+FSL+ALGRMSDVWG 
Sbjct: 390 KLVYFHGALCETLRLYPPVPFQHKIPSQPDVLPSGHHVNPKTKIIFSLYALGRMSDVWGK 449

Query: 425 DCMDFKPERWISERGSIKHVPSYKFLAFNAGPRTCLGKEVAFLEMKIVAAAIIHNYN-VE 484
           DCM+FKPERWISE G IKHVPSYKFLAFNAGPRTCLGK+VAF+EMKIVAAA+IHNY  V+
Sbjct: 450 DCMEFKPERWISETGMIKHVPSYKFLAFNAGPRTCLGKQVAFIEMKIVAAAMIHNYAIVQ 509

Query: 485 LIGHKVVPSASIILHMKHGFKIKISRRW 506
             G  VVPSASIILHMKHGFK+++++RW
Sbjct: 510 QTGQAVVPSASIILHMKHGFKVRVTKRW 535

BLAST of Sed0012335 vs. NCBI nr
Match: XP_022977942.1 (alkane hydroxylase MAH1-like [Cucurbita maxima])

HSP 1 Score: 775.0 bits (2000), Expect = 4.0e-220
Identity = 369/497 (74.25%), Postives = 430/497 (86.52%), Query Frame = 0

Query: 12  STLLPFLPFITFSIFFVFLKWNPRGIPWNWPFLGMLPTVALNIHRIHDRVTEILNQAGST 71
           S LL F+PF+ F++F + LKWN  G+PWNWPF GM PT+  +IHR+H RVT IL Q+ ST
Sbjct: 8   SILLAFIPFV-FALFVLSLKWNFNGLPWNWPFFGMTPTILFHIHRVHHRVTRILQQSPST 67

Query: 72  FFFKGLWFSGLDFFLTADPSNIHHILSTNFERYPKGPDFKYIFDVLGDGIFNSDSVSWQS 131
           F FKG WF+GLDFFLTADPSNIHHILSTNF+RYPKGPDFKYIFDVLGDGIFNSDSVSW++
Sbjct: 68  FLFKGAWFAGLDFFLTADPSNIHHILSTNFDRYPKGPDFKYIFDVLGDGIFNSDSVSWKT 127

Query: 132 QRRTAHALLRTHSFLQFSEEITIKKVKQGLIPILESVSEHGSVLDLQDLFQRFSFDSTCM 191
           QR TAHAL+R   FL F E+I +KKV++GL+ +LESV E+GSVLDLQDLFQRFSFDSTCM
Sbjct: 128 QRTTAHALVRQEKFLHFLEKIAMKKVREGLVSVLESVCENGSVLDLQDLFQRFSFDSTCM 187

Query: 192 LVTGFDLCSLSLEFPEIPFSKAMDDVEEVIFLRHFFPKKLWEFQSKFQIGQPKRLKQAWE 251
           LVTGFDL SLSLEFP++PFSKAMDD+EEVIFLRHF PKK+WEFQ K QIG P RL++AWE
Sbjct: 188 LVTGFDLHSLSLEFPQLPFSKAMDDIEEVIFLRHFIPKKIWEFQKKLQIGHPMRLQEAWE 247

Query: 252 IIDQTIAKLLASKRESLKMMMIMKEES--DMITSYLTDYKHDDDKFLRDTILNITIAGRD 311
           IIDQTIA L+A+KRESL  +   K++   D+ITSY+ + K +DDK LRDT+LN  IAGRD
Sbjct: 248 IIDQTIANLIATKRESLNQLKEEKDQEGVDLITSYM-ENKENDDKMLRDTVLNFMIAGRD 307

Query: 312 TLSSALSWFFFCLSNNPTVEAKIREELKTAIPTNEGQNQQWIFPIEEVKKLVYFHGALCE 371
           TLSSALSWFFFCLSNNPT EAKIREEL+T IP +E + Q  +F I+EV KLVYFHGALCE
Sbjct: 308 TLSSALSWFFFCLSNNPTAEAKIREELETTIPRSEPRGQWRMFSIKEVDKLVYFHGALCE 367

Query: 372 TLRLYPPVPFQHKVPSQTDILPSGHYVNPKTKILFSLHALGRMSDVWGNDCMDFKPERWI 431
           TLRLYPPVPFQHK+PSQ D+LPSG +VNPKTKI+FSL+ALGRMSDVWG DCM+FKPERWI
Sbjct: 368 TLRLYPPVPFQHKIPSQPDVLPSGLHVNPKTKIIFSLYALGRMSDVWGKDCMEFKPERWI 427

Query: 432 SERGSIKHVPSYKFLAFNAGPRTCLGKEVAFLEMKIVAAAIIHNYN-VELIGHKVVPSAS 491
           +E G IKHVPSYKF+AFNAGPRTC+GK+VAF EMKIVAAA+IHNY  V+  G +VVPSAS
Sbjct: 428 AETGRIKHVPSYKFVAFNAGPRTCIGKQVAFTEMKIVAAAMIHNYAIVQETGQEVVPSAS 487

Query: 492 IILHMKHGFKIKISRRW 506
           IILHMKHGFK+++++RW
Sbjct: 488 IILHMKHGFKVRVTKRW 502

BLAST of Sed0012335 vs. NCBI nr
Match: KAG7034398.1 (Alkane hydroxylase MAH1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 774.6 bits (1999), Expect = 5.2e-220
Identity = 369/497 (74.25%), Postives = 429/497 (86.32%), Query Frame = 0

Query: 12  STLLPFLPFITFSIFFVFLKWNPRGIPWNWPFLGMLPTVALNIHRIHDRVTEILNQAGST 71
           S LL F+PF+ F++F + LKWN  G+PWNWPFLGM PT+  +IHR+H  +T IL Q+ ST
Sbjct: 8   SILLAFIPFV-FALFVLSLKWNFNGLPWNWPFLGMTPTILFHIHRVHHHLTRILQQSPST 67

Query: 72  FFFKGLWFSGLDFFLTADPSNIHHILSTNFERYPKGPDFKYIFDVLGDGIFNSDSVSWQS 131
           F FKG WF+GLDFFLTADPSNIHHILSTNF+RYPKG DFKY+FDVLGDGI NSDSVSW++
Sbjct: 68  FLFKGAWFAGLDFFLTADPSNIHHILSTNFDRYPKGTDFKYMFDVLGDGISNSDSVSWKT 127

Query: 132 QRRTAHALLRTHSFLQFSEEITIKKVKQGLIPILESVSEHGSVLDLQDLFQRFSFDSTCM 191
           QR TAHAL+R   FL F E I +KKV++GL+ +LESV E+GSVLDLQDLFQRFSFDS+CM
Sbjct: 128 QRTTAHALVRQEKFLHFLERIAMKKVREGLVSVLESVCENGSVLDLQDLFQRFSFDSSCM 187

Query: 192 LVTGFDLCSLSLEFPEIPFSKAMDDVEEVIFLRHFFPKKLWEFQSKFQIGQPKRLKQAWE 251
           LVTG DL SLSLEFP++PFSKAMDD+EEVIFLRH  PKK+WEFQ K QIG PKRL++AWE
Sbjct: 188 LVTGLDLHSLSLEFPQVPFSKAMDDIEEVIFLRHLIPKKIWEFQKKLQIGHPKRLQEAWE 247

Query: 252 IIDQTIAKLLASKRESLKMMMIMKEES--DMITSYLTDYKHDDDKFLRDTILNITIAGRD 311
           IIDQTIA L+A+KRESL  +   K++   D+ITSY+ + K +DDK LRDT+LN  IAGRD
Sbjct: 248 IIDQTIANLIATKRESLNQLREEKDQEGVDLITSYM-ENKENDDKMLRDTVLNFVIAGRD 307

Query: 312 TLSSALSWFFFCLSNNPTVEAKIREELKTAIPTNEGQNQQWIFPIEEVKKLVYFHGALCE 371
           TLSSALSWFFFCLSNNPTVEAKIREEL+T IPT+E + Q  +F I+EV KLVYFHGALCE
Sbjct: 308 TLSSALSWFFFCLSNNPTVEAKIREELETTIPTSEPRGQWRMFSIKEVDKLVYFHGALCE 367

Query: 372 TLRLYPPVPFQHKVPSQTDILPSGHYVNPKTKILFSLHALGRMSDVWGNDCMDFKPERWI 431
           TLRLYPPVPFQHK+PSQ D+LPSGH+VNPKTKI+FSL+ALGRMSDVWG DCM+FKPERWI
Sbjct: 368 TLRLYPPVPFQHKIPSQPDVLPSGHHVNPKTKIIFSLYALGRMSDVWGKDCMEFKPERWI 427

Query: 432 SERGSIKHVPSYKFLAFNAGPRTCLGKEVAFLEMKIVAAAIIHNYN-VELIGHKVVPSAS 491
           SE G IKHVPSYKFLAFNAGPRTCLGK+VAF+EMKIVAAA+IHNY  V+  G  VVPSAS
Sbjct: 428 SETGMIKHVPSYKFLAFNAGPRTCLGKQVAFIEMKIVAAAMIHNYAIVQQTGQAVVPSAS 487

Query: 492 IILHMKHGFKIKISRRW 506
           IILHMKHGFK+++++RW
Sbjct: 488 IILHMKHGFKVRVTKRW 502

BLAST of Sed0012335 vs. NCBI nr
Match: XP_023544417.1 (alkane hydroxylase MAH1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 773.5 bits (1996), Expect = 1.2e-219
Identity = 368/497 (74.04%), Postives = 429/497 (86.32%), Query Frame = 0

Query: 12  STLLPFLPFITFSIFFVFLKWNPRGIPWNWPFLGMLPTVALNIHRIHDRVTEILNQAGST 71
           S LL F+PF+ F++  + LKWN  G+PWNWPF GM PT+  +IHR+H R+T IL Q+ ST
Sbjct: 8   SILLAFIPFV-FALVVLSLKWNFNGLPWNWPFFGMTPTILFHIHRVHHRLTHILQQSPST 67

Query: 72  FFFKGLWFSGLDFFLTADPSNIHHILSTNFERYPKGPDFKYIFDVLGDGIFNSDSVSWQS 131
           F FKG WF+GLDFFLT DPSNIHHILSTNF+RYPKGPDFKYIFDVLGDGIFNSDSVSW++
Sbjct: 68  FLFKGAWFAGLDFFLTVDPSNIHHILSTNFDRYPKGPDFKYIFDVLGDGIFNSDSVSWKT 127

Query: 132 QRRTAHALLRTHSFLQFSEEITIKKVKQGLIPILESVSEHGSVLDLQDLFQRFSFDSTCM 191
           QR TAHAL+R   FL F E+I +KKV++ L+ +LESV E+GSVLDLQDLFQRFSFDSTCM
Sbjct: 128 QRTTAHALVRQEKFLHFLEKIAMKKVREELVSVLESVCENGSVLDLQDLFQRFSFDSTCM 187

Query: 192 LVTGFDLCSLSLEFPEIPFSKAMDDVEEVIFLRHFFPKKLWEFQSKFQIGQPKRLKQAWE 251
           LVTGFDL SLSLEFP++PFSKAMDD+EEVIFLRHF PKK+WEFQ K QIG P RL++AWE
Sbjct: 188 LVTGFDLHSLSLEFPQVPFSKAMDDIEEVIFLRHFIPKKIWEFQKKLQIGHPMRLQEAWE 247

Query: 252 IIDQTIAKLLASKRESLKMMMIMKEES--DMITSYLTDYKHDDDKFLRDTILNITIAGRD 311
           IIDQTIA L+A+KRESL  +   K+E   D+ITSY+ + K +DDK LRDT+LN  IAGRD
Sbjct: 248 IIDQTIANLIATKRESLNQLREEKDEEGVDLITSYM-ENKENDDKMLRDTVLNFMIAGRD 307

Query: 312 TLSSALSWFFFCLSNNPTVEAKIREELKTAIPTNEGQNQQWIFPIEEVKKLVYFHGALCE 371
           TLSSALSWFFFCLSNNPTVEAKIREEL+T IPT+E + Q  +F I+EV KLVYFHGALCE
Sbjct: 308 TLSSALSWFFFCLSNNPTVEAKIREELETTIPTSEPRGQWRMFSIKEVDKLVYFHGALCE 367

Query: 372 TLRLYPPVPFQHKVPSQTDILPSGHYVNPKTKILFSLHALGRMSDVWGNDCMDFKPERWI 431
           TLRLYPPVPFQHK+PSQ D+LPSGH+VNPKTKI+FSL+ALGRMSDVWG DCM+FKPERW+
Sbjct: 368 TLRLYPPVPFQHKIPSQPDVLPSGHHVNPKTKIIFSLYALGRMSDVWGKDCMEFKPERWV 427

Query: 432 SERGSIKHVPSYKFLAFNAGPRTCLGKEVAFLEMKIVAAAIIHNYN-VELIGHKVVPSAS 491
           SE+G I HVPS+KFLAFNAGPRTCLGK+VAF EMKIVAAA+IHNY  V+  G +VVPSAS
Sbjct: 428 SEKGRIMHVPSHKFLAFNAGPRTCLGKQVAFTEMKIVAAAMIHNYAIVQETGQEVVPSAS 487

Query: 492 IILHMKHGFKIKISRRW 506
           IILHMKHG K+++++RW
Sbjct: 488 IILHMKHGLKVRVTKRW 502

BLAST of Sed0012335 vs. NCBI nr
Match: XP_038882620.1 (alkane hydroxylase MAH1-like isoform X1 [Benincasa hispida])

HSP 1 Score: 767.7 bits (1981), Expect = 6.3e-218
Identity = 374/498 (75.10%), Postives = 428/498 (85.94%), Query Frame = 0

Query: 17  FLPFITFSIFFVFLKWNPRG-IPWNWPFLGMLPTVALNIHRIHDRVTEILNQAGSTFFFK 76
           F+PF+ F IF    +WN  G +PWNWP +GM PTV  +I+R HDRVTE+L  AGSTFFFK
Sbjct: 14  FIPFL-FIIFSRISRWNFHGVVPWNWPIVGMTPTVVAHINRAHDRVTEVLQAAGSTFFFK 73

Query: 77  GLWFSGLDFFLTADPSNIHHILSTNFERYPKGPDFKYIFDVLGDGIFNSDSVSWQSQRRT 136
           G+WFSG+DF  T DPSNIHHILS+NFERYPKGPDFKYIF+VLGDGIFNSDS  W++QR+T
Sbjct: 74  GVWFSGMDFLFTVDPSNIHHILSSNFERYPKGPDFKYIFEVLGDGIFNSDSDVWKNQRKT 133

Query: 137 AHALLRTHSFLQFSEEITIKKVKQGLIPILESVSEHGSVLDLQDLFQRFSFDSTCMLVTG 196
           AH+L+   +FLQF E+IT+KKVK+GL+PILESV E+GSVLDLQDLFQRFSFDSTCMLVTG
Sbjct: 134 AHSLVHDENFLQFLEKITLKKVKEGLVPILESVCENGSVLDLQDLFQRFSFDSTCMLVTG 193

Query: 197 FDLCSLSLEFPEIPFSKAMDDVEEVIFLRHFFPKKLWEFQSKFQIGQPKRLKQAWEIIDQ 256
           FDL SLSL+ PE+PFSKAMDDVEEVIFLRH FPKK+WEF  K QIGQPKRLKQAWEIID+
Sbjct: 194 FDLNSLSLQLPEVPFSKAMDDVEEVIFLRHVFPKKIWEFHKKLQIGQPKRLKQAWEIIDE 253

Query: 257 TIAKLLASKRESLKMMMIMKEES-------DMITSYLTDYKHDDDKFLRDTILNITIAGR 316
           TIAKL+ASKR+S+K    MKEE+       D+ITSY+T+ K+DD+ FLRDT+LN  IAGR
Sbjct: 254 TIAKLIASKRKSMKNQ--MKEEADEQGIGVDLITSYITNTKNDDE-FLRDTVLNFMIAGR 313

Query: 317 DTLSSALSWFFFCLSNNPTVEAKIREELKTAIPTNEGQNQQWIFPIEEVKKLVYFHGALC 376
           DTLSSALSWFFFCLSNNP V A IREELKT IPTNE ++Q  IF IEEV KLVYFHG+LC
Sbjct: 314 DTLSSALSWFFFCLSNNPIVVANIREELKTTIPTNEVRDQLRIFSIEEVDKLVYFHGSLC 373

Query: 377 ETLRLYPPVPFQHKVPSQTDILPSGHYVNPKTKILFSLHALGRMSDVWGNDCMDFKPERW 436
           E LRLYPPVP QHKV  Q DILP+GH++NPKTKIL SL+ALGRMSDVWG D M+FKPERW
Sbjct: 374 EALRLYPPVPLQHKVSVQHDILPTGHHINPKTKILISLYALGRMSDVWGKDSMEFKPERW 433

Query: 437 ISERGSIKHVPSYKFLAFNAGPRTCLGKEVAFLEMKIVAAAIIHNYN-VELIGHKVVPSA 496
           ISE G IKHVPSYKFLAFNAGPRTCLGK+VAF E+KIVAAAIIHNYN ++  GHKVVP+A
Sbjct: 434 ISENGRIKHVPSYKFLAFNAGPRTCLGKQVAFTELKIVAAAIIHNYNIIQQAGHKVVPNA 493

Query: 497 SIILHMKHGFKIKISRRW 506
           SIILHMKHGFK+K+++RW
Sbjct: 494 SIILHMKHGFKVKVTKRW 507

BLAST of Sed0012335 vs. ExPASy Swiss-Prot
Match: Q9FVS9 (Alkane hydroxylase MAH1 OS=Arabidopsis thaliana OX=3702 GN=CYP96A15 PE=2 SV=1)

HSP 1 Score: 468.0 bits (1203), Expect = 1.4e-130
Identity = 230/499 (46.09%), Postives = 324/499 (64.93%), Query Frame = 0

Query: 17  FLPFITFSIFFVFLKWNPRGIP--WNWPFLGMLPTVALNIHRIHDRVTEILNQAGSTFFF 76
           F+ F+     + FL+  P+G P   NWPFL MLP +   I RI+D   E+L     TF+F
Sbjct: 10  FIFFLVCLFTYFFLQKKPQGQPILKNWPFLRMLPGMLHQIPRIYDWTVEVLEATNLTFYF 69

Query: 77  KGLWFSGLDFFLTADPSNIHHILSTNFERYPKGPDFKYIFDVLGDGIFNSDSVSWQSQRR 136
           KG W SG D   TADP NIHHILS+NF  YPKGP+FK IFDVLG+GI   D   W+  R+
Sbjct: 70  KGPWLSGTDMLFTADPRNIHHILSSNFGNYPKGPEFKKIFDVLGEGILTVDFELWEEMRK 129

Query: 137 TAHALLRTHSFLQFSEEITIKKVKQGLIPILESVSEHGSVLDLQDLFQRFSFDSTCMLVT 196
           + HAL     F++ S      K+K+GL+P L++ ++   +++LQD+FQRF FD++ +L+T
Sbjct: 130 SNHALFHNQDFIELSVSSNKSKLKEGLVPFLDNAAQKNIIIELQDVFQRFMFDTSSILMT 189

Query: 197 GFDLCSLSLEFPEIPFSKAMDDVEEVIFLRHFFPKKLWEFQSKFQIGQPKRLKQAWEIID 256
           G+D  SLS+E  E+ F +A D  EE I+ RHF P  LW  Q+   IG  ++++ A   ++
Sbjct: 190 GYDPMSLSIEMLEVEFGEAADIGEEAIYYRHFKPVILWRLQNWIGIGLERKMRTALATVN 249

Query: 257 QTIAKLLASKR-ESLKMMMIMKEESDMITSYLTD-------YKHDDDKFLRDTILNITIA 316
           +  AK+++S+R E +          D +T Y+          K + DKF+RD I ++ +A
Sbjct: 250 RMFAKIISSRRKEEISRAKTEPYSKDALTYYMNVDTSKYKLLKPNKDKFIRDVIFSLVLA 309

Query: 317 GRDTLSSALSWFFFCLSNNPTVEAKIREELKTAIPTNEGQNQQWIFPIEEVKKLVYFHGA 376
           GRDT SS L+WFF+ LS +P V AK+R E+ T             F  E+++KLVY H A
Sbjct: 310 GRDTTSSVLTWFFWLLSKHPQVMAKLRHEINTK------------FDNEDLEKLVYLHAA 369

Query: 377 LCETLRLYPPVPFQHKVPSQTDILPSGHYVNPKTKILFSLHALGRMSDVWGNDCMDFKPE 436
           L E++RLYPP+PF HK P++ D+LPSGH V+  +KI+  ++ALGRM  VWG D +DFKPE
Sbjct: 370 LSESMRLYPPLPFNHKSPAKPDVLPSGHKVDANSKIVICIYALGRMRSVWGEDALDFKPE 429

Query: 437 RWISERGSIKHVPSYKFLAFNAGPRTCLGKEVAFLEMKIVAAAIIHNYNVELI-GHKVVP 496
           RWIS+ G ++H PSYKF+AFN+GPRTCLGK +A L+MK+VA  II NY+ ++I GHKV P
Sbjct: 430 RWISDNGGLRHEPSYKFMAFNSGPRTCLGKNLALLQMKMVALEIIRNYDFKVIEGHKVEP 489

Query: 497 SASIILHMKHGFKIKISRR 505
             SI+L MKHG K+ ++++
Sbjct: 490 IPSILLRMKHGLKVTVTKK 496

BLAST of Sed0012335 vs. ExPASy Swiss-Prot
Match: A0A140IL90 (Noroxomaritidine synthase OS=Narcissus aff. pseudonarcissus MK-2014 OX=1540222 GN=Cyp96T1 PE=1 SV=1)

HSP 1 Score: 403.7 bits (1036), Expect = 3.2e-111
Identity = 197/497 (39.64%), Postives = 299/497 (60.16%), Query Frame = 0

Query: 14  LLPFLPFITFSIFFVFLKWNPRGIPWNWPFLGMLPTVALNIHRIHDRVTEILNQAGSTFF 73
           L+    F+ FS+       +   +P+NWP  GMLP +  N ++ +D  T  L + G TF 
Sbjct: 19  LIAIACFLIFSLLLSARSSSEDSLPYNWPIFGMLPAIISN-NQFNDFTTARLRKMGWTFI 78

Query: 74  FKGLWFSGLDFFLTADPSNIHHILSTNFERYPKGPDFKYIFDVLGDGIFNSDSVSWQSQR 133
           FKG W   +D+  T DPSNI+H+ + NFE YPKG +   +FD+ G+ IFN+D   W   R
Sbjct: 79  FKGPWLLDMDYIFTCDPSNINHMFNDNFENYPKG-ELGKVFDIFGNNIFNADGDLWHDHR 138

Query: 134 RTAHALLRTHSFLQFSEEITIKKVKQGLIPILESVSEHGSVLDLQDLFQRFSFDSTCMLV 193
           + A  +L   ++          K+   LIPIL+S +     +DLQD+F RF+FD++C  V
Sbjct: 139 KMAQTILWDGNYRTMQATFIRNKMDNALIPILDSAASKRKPVDLQDVFFRFTFDTSCFSV 198

Query: 194 TGFDLCSLSLEFPEIPFSKAMDDVEEVIFLRHFFPKKLWEFQSKFQIGQPKRLKQAWEII 253
              D  SL++EFP +PFSKA D   +    RH  P+ +W+ +  F +G  + L  AW++I
Sbjct: 199 LAADPESLTMEFPAVPFSKAADQALDAALTRHITPRLIWKLKRFFNVGSERTLAVAWKVI 258

Query: 254 DQTIAKLLASKRESLKMMMIMKEESDMITSYLTDYKHDDDKFLRDTILNITIAGRDTLSS 313
           D  I   +A  +   K++  +    D ++ Y+ ++   DDKFLRD      +A R+T S 
Sbjct: 259 DSYIYDKIAELKAKRKLVGKI-NSYDAVSFYMDNFNIHDDKFLRDNAFTYLLAQRNTQSL 318

Query: 314 ALSWFFFCLSNNPTVEAKIREELKTAIPTNEGQNQQW-----IFPIEEVKKLVYFHGALC 373
            ++W F+ L  NP VE KI  ELK+ +  +E   +++     +F    ++  +Y H  LC
Sbjct: 319 TMTWLFYALFENPKVELKILSELKSIV--DESSERKFNDGFALFDSNMIQSAIYLHATLC 378

Query: 374 ETLRLYPPVPFQHKVPSQTDILPSGHYVNPKTKILFSLHALGRMSDVWGNDCMDFKPERW 433
           E LR+YPPVPF+ K   + D+LPSGH V    KILFS +A+ RM  +WG+DC++FKPERW
Sbjct: 379 EALRIYPPVPFEIKDAHKADVLPSGHKVRAGEKILFSPYAMARMKGIWGDDCLEFKPERW 438

Query: 434 ISERGSIKHVPSYKFLAFNAGPRTCLGKEVAFLEMKIVAAAIIHNYNVELI-GHKVVPSA 493
           I+  G++KH P+YKF AF+AGPR CLGKE++F +MK+V A II+N++++++ GH V  S 
Sbjct: 439 ITGNGTLKHEPAYKFFAFSAGPRICLGKELSFTQMKMVVATIIYNFHLQMVKGHVVEQSN 498

Query: 494 SIILHMKHGFKIKISRR 505
           SI++ MKHG  +++ +R
Sbjct: 499 SILMDMKHGLMVQVRKR 510

BLAST of Sed0012335 vs. ExPASy Swiss-Prot
Match: A0A140IL92 (Noroxomaritidine synthase 3 OS=Narcissus aff. pseudonarcissus MK-2014 OX=1540222 GN=Cyp96T3 PE=2 SV=1)

HSP 1 Score: 402.9 bits (1034), Expect = 5.4e-111
Identity = 197/497 (39.64%), Postives = 298/497 (59.96%), Query Frame = 0

Query: 14  LLPFLPFITFSIFFVFLKWNPRGIPWNWPFLGMLPTVALNIHRIHDRVTEILNQAGSTFF 73
           L+    F+ FS+       +   +P+NWP  GMLP +  N  + +D  T  L + G TF 
Sbjct: 19  LIAIACFLIFSLLLSARSSSEDSLPYNWPIFGMLPAIISN-SQFNDFTTARLRKMGWTFI 78

Query: 74  FKGLWFSGLDFFLTADPSNIHHILSTNFERYPKGPDFKYIFDVLGDGIFNSDSVSWQSQR 133
           FKG W   +D+  T DPSNI+H+ + NFE YPKG +   +FD+ G+ IFN+D   W   R
Sbjct: 79  FKGPWLLDMDYIFTCDPSNINHMFNDNFENYPKG-ELGKVFDIFGNNIFNADGDLWHDHR 138

Query: 134 RTAHALLRTHSFLQFSEEITIKKVKQGLIPILESVSEHGSVLDLQDLFQRFSFDSTCMLV 193
           + A  +L   ++          K+   LIPIL+S +     +DLQD+F RF+FD++C  V
Sbjct: 139 KMAQTILWDGNYRTMQATFIRNKMDNALIPILDSAASKRKPVDLQDVFFRFTFDTSCFSV 198

Query: 194 TGFDLCSLSLEFPEIPFSKAMDDVEEVIFLRHFFPKKLWEFQSKFQIGQPKRLKQAWEII 253
              D  SL++EFP +PFSKA D   +    RH  P+ +W+ +  F +G  + L  AW++I
Sbjct: 199 LAADPESLTMEFPAVPFSKAADQALDAALTRHITPRLIWKLKRFFNVGSERTLAVAWKVI 258

Query: 254 DQTIAKLLASKRESLKMMMIMKEESDMITSYLTDYKHDDDKFLRDTILNITIAGRDTLSS 313
           D  I   +A  +   K++  +    D ++ Y+ ++   DDKFLRD      +A R+T S 
Sbjct: 259 DSYIYDKIAELKAKRKLVGKI-NSYDAVSFYMDNFNIHDDKFLRDNAFTYLLAQRNTQSL 318

Query: 314 ALSWFFFCLSNNPTVEAKIREELKTAIPTNEGQNQQW-----IFPIEEVKKLVYFHGALC 373
            ++W F+ L  NP VE KI  ELK+ +  +E   +++     +F    ++  +Y H  LC
Sbjct: 319 TMAWLFYALFENPKVELKILSELKSIV--DESSERRFNDGFALFDSNMIQSAIYLHATLC 378

Query: 374 ETLRLYPPVPFQHKVPSQTDILPSGHYVNPKTKILFSLHALGRMSDVWGNDCMDFKPERW 433
           E LR+YPPVPF+ K   + D+LPSGH V    KILFS +A+ RM  +WG+DC++FKPERW
Sbjct: 379 EALRIYPPVPFEIKDAHKADVLPSGHKVRAGEKILFSPYAMARMKGIWGDDCLEFKPERW 438

Query: 434 ISERGSIKHVPSYKFLAFNAGPRTCLGKEVAFLEMKIVAAAIIHNYNVELI-GHKVVPSA 493
           I+  G++KH P+YKF AF+AGPR CLGKE++F +MK+V A II+N++++++ GH V  S 
Sbjct: 439 ITGNGTLKHEPAYKFFAFSAGPRICLGKELSFTQMKMVVATIIYNFHLQMVKGHVVEQSN 498

Query: 494 SIILHMKHGFKIKISRR 505
           SI++ MKHG  +++ +R
Sbjct: 499 SILMDMKHGLMVQVRKR 510

BLAST of Sed0012335 vs. ExPASy Swiss-Prot
Match: A0A140IL91 (Noroxomaritidine synthase 2 OS=Narcissus aff. pseudonarcissus MK-2014 OX=1540222 GN=Cyp96T2 PE=2 SV=1)

HSP 1 Score: 401.7 bits (1031), Expect = 1.2e-110
Identity = 197/497 (39.64%), Postives = 299/497 (60.16%), Query Frame = 0

Query: 14  LLPFLPFITFSIFFVFLKWNPRGIPWNWPFLGMLPTVALNIHRIHDRVTEILNQAGSTFF 73
           L+    F+ FS+       +   +P+NWP  GMLP +  N ++ +D  T  L + G TF 
Sbjct: 19  LIAIACFLIFSLLLSARSSSEDSLPYNWPIFGMLPAIISN-NQFNDFTTARLRKMGWTFI 78

Query: 74  FKGLWFSGLDFFLTADPSNIHHILSTNFERYPKGPDFKYIFDVLGDGIFNSDSVSWQSQR 133
           FKG W   +D+  T DPSNI+H+ + NFE YPKG +   +FD+ G+ IFN+D   W   R
Sbjct: 79  FKGPWLLDMDYIFTCDPSNINHMFNDNFENYPKG-ELGKVFDIFGNNIFNADGDLWHDHR 138

Query: 134 RTAHALLRTHSFLQFSEEITIKKVKQGLIPILESVSEHGSVLDLQDLFQRFSFDSTCMLV 193
           + A  +L   ++          K+   LIPIL+S +     +DLQD+  RF+FD++C  V
Sbjct: 139 KMAQTILWDGNYRTMQATFIRNKMDNALIPILDSAACKRKPVDLQDVLLRFTFDTSCFSV 198

Query: 194 TGFDLCSLSLEFPEIPFSKAMDDVEEVIFLRHFFPKKLWEFQSKFQIGQPKRLKQAWEII 253
              D  SL++EFP +PFSKA D   +    RH  P+ +W+ +  F +G  + L  AW++I
Sbjct: 199 LAADPESLTMEFPPVPFSKAADQALDAALTRHITPRLIWKLKRFFNVGSERTLAVAWKVI 258

Query: 254 DQTIAKLLASKRESLKMMMIMKEESDMITSYLTDYKHDDDKFLRDTILNITIAGRDTLSS 313
           D  I   +A  +   K++  +    D ++ Y+ ++   DDKFLRD      +A R+T S 
Sbjct: 259 DSYIYDKIAELKAKRKLVGKI-NSYDAVSFYMDNFNIHDDKFLRDNAFTYLLAQRNTQSL 318

Query: 314 ALSWFFFCLSNNPTVEAKIREELKTAIPTNEGQNQQW-----IFPIEEVKKLVYFHGALC 373
            ++W F+ L  NP VE KI  ELK+ +  +E   +++     +F    ++  +Y H ALC
Sbjct: 319 TMTWLFYALFENPKVELKILSELKSIV--DESSERKFNDGFTLFDSNMIQSAIYLHAALC 378

Query: 374 ETLRLYPPVPFQHKVPSQTDILPSGHYVNPKTKILFSLHALGRMSDVWGNDCMDFKPERW 433
           E LR+YPPVPF+ K   + D+LPSGH V    KILFS +A+ RM  +WG+DC++FKPERW
Sbjct: 379 EALRIYPPVPFEIKDAHKADVLPSGHKVRAGEKILFSPYAMARMKGIWGDDCLEFKPERW 438

Query: 434 ISERGSIKHVPSYKFLAFNAGPRTCLGKEVAFLEMKIVAAAIIHNYNVELI-GHKVVPSA 493
           I+  G++KH P+YKF AF+AGPR CLGKE++F +MK+V A II+N++++++ GH V  S 
Sbjct: 439 ITGNGTLKHEPAYKFFAFSAGPRICLGKELSFTQMKMVVATIIYNFHLQMVKGHVVEQSN 498

Query: 494 SIILHMKHGFKIKISRR 505
           SI++ MKHG  +++ +R
Sbjct: 499 SILMDMKHGLMVQVRKR 510

BLAST of Sed0012335 vs. ExPASy Swiss-Prot
Match: A0A2H5AJ00 (Noroxomaritidine synthase 1 OS=Narcissus pseudonarcissus OX=39639 GN=Cyp96T1 PE=2 SV=1)

HSP 1 Score: 400.2 bits (1027), Expect = 3.5e-110
Identity = 196/497 (39.44%), Postives = 299/497 (60.16%), Query Frame = 0

Query: 14  LLPFLPFITFSIFFVFLKWNPRGIPWNWPFLGMLPTVALNIHRIHDRVTEILNQAGSTFF 73
           L+     + FS+       +  G+P+NWP  GMLP +  N ++ +D  T  L + G TF 
Sbjct: 19  LIAIACLVVFSLLRSARSSSKGGLPYNWPIFGMLPAIISN-NQFNDFTTARLRKMGWTFI 78

Query: 74  FKGLWFSGLDFFLTADPSNIHHILSTNFERYPKGPDFKYIFDVLGDGIFNSDSVSWQSQR 133
           FKG W   +D+  T DPSNI+H+ + NFE YPKG +   +FD+ G+ IFN+D   W   R
Sbjct: 79  FKGPWLLDMDYIFTCDPSNINHMFNDNFENYPKG-ELGKVFDIFGNNIFNADGDLWHDHR 138

Query: 134 RTAHALLRTHSFLQFSEEITIKKVKQGLIPILESVSEHGSVLDLQDLFQRFSFDSTCMLV 193
           + A  +L   ++          K+   LIPIL+S +   + +DLQD+  RF+FD++C  V
Sbjct: 139 KMAQTILWDGNYRTMQATFIRNKMDNALIPILDSAACKRNPVDLQDVLLRFTFDTSCFSV 198

Query: 194 TGFDLCSLSLEFPEIPFSKAMDDVEEVIFLRHFFPKKLWEFQSKFQIGQPKRLKQAWEII 253
              D  SL++EFP +PFSKA D   +    RH  P+ +W+ +  F +G  + L  AW++I
Sbjct: 199 LAADPESLTMEFPPVPFSKAADQALDAALTRHITPRLIWKLKRFFNVGSERTLAVAWKVI 258

Query: 254 DQTIAKLLASKRESLKMMMIMKEESDMITSYLTDYKHDDDKFLRDTILNITIAGRDTLSS 313
           D  I   +A  +   K++  +    D ++ Y+ ++   DDKFLRD      +A R+T S 
Sbjct: 259 DSYIYDKIAELKAKRKLVGKI-NSYDAVSFYMDNFNIHDDKFLRDNAFTYLLAQRNTQSL 318

Query: 314 ALSWFFFCLSNNPTVEAKIREELKTAIPTNEGQNQQW-----IFPIEEVKKLVYFHGALC 373
            ++W F+ L  NP VE KI  ELK+ +  +E   +++     +F    ++  +Y H  LC
Sbjct: 319 TMTWLFYALFENPKVELKILSELKSIV--DESSERKFNDGFALFDSNMIQSAIYLHATLC 378

Query: 374 ETLRLYPPVPFQHKVPSQTDILPSGHYVNPKTKILFSLHALGRMSDVWGNDCMDFKPERW 433
           E LR+YPPVPF+ K   + D+LPSGH V    KILFS +A+ RM  +WG+DC++FKPERW
Sbjct: 379 EALRIYPPVPFEIKDAHKADVLPSGHKVRAGEKILFSPYAMARMKGIWGDDCLEFKPERW 438

Query: 434 ISERGSIKHVPSYKFLAFNAGPRTCLGKEVAFLEMKIVAAAIIHNYNVELI-GHKVVPSA 493
           I+  G++KH P+YKF AF+AGPR CLGKE++F +MK+V A II+N++++++ GH V  S 
Sbjct: 439 ITGNGTLKHEPAYKFFAFSAGPRICLGKELSFTQMKMVVATIIYNFHLQMVKGHVVEQSN 498

Query: 494 SIILHMKHGFKIKISRR 505
           SI++ MKHG  +++ +R
Sbjct: 499 SILMDMKHGLMVQVRKR 510

BLAST of Sed0012335 vs. ExPASy TrEMBL
Match: A0A6J1GEE6 (alkane hydroxylase MAH1-like OS=Cucurbita moschata OX=3662 GN=LOC111453415 PE=3 SV=1)

HSP 1 Score: 778.5 bits (2009), Expect = 1.7e-221
Identity = 373/508 (73.43%), Postives = 434/508 (85.43%), Query Frame = 0

Query: 5   CRPPMA----FSTLLPFLPFITFSIFFVFLKWNPRGIPWNWPFLGMLPTVALNIHRIHDR 64
           CR PMA     S LL F+PF+ F++F + LKWN  G+PWNWPF GM PT+  +IHR+H R
Sbjct: 30  CRAPMASLSFLSILLAFIPFV-FALFVLSLKWNFNGLPWNWPFFGMTPTILFHIHRVHHR 89

Query: 65  VTEILNQAGSTFFFKGLWFSGLDFFLTADPSNIHHILSTNFERYPKGPDFKYIFDVLGDG 124
           +T IL Q+ STF FKG WF+GLDFFLTADPSNIHHILSTNF+RYPKG DFKY+FDVLGDG
Sbjct: 90  LTRILQQSPSTFLFKGAWFAGLDFFLTADPSNIHHILSTNFDRYPKGTDFKYMFDVLGDG 149

Query: 125 IFNSDSVSWQSQRRTAHALLRTHSFLQFSEEITIKKVKQGLIPILESVSEHGSVLDLQDL 184
           I NSDSVSW++QR TAHAL+R   FL F E I +KKV++GL+ +LESV E+GSVLDLQDL
Sbjct: 150 ISNSDSVSWKTQRTTAHALVRQEKFLHFLERIAMKKVREGLVSVLESVCENGSVLDLQDL 209

Query: 185 FQRFSFDSTCMLVTGFDLCSLSLEFPEIPFSKAMDDVEEVIFLRHFFPKKLWEFQSKFQI 244
           FQRFSFDS+CMLVTG DL SLSLEFP++PFSKAMDD+EEVIFLRH  PKK+WEFQ K QI
Sbjct: 210 FQRFSFDSSCMLVTGLDLHSLSLEFPQVPFSKAMDDIEEVIFLRHLIPKKIWEFQKKLQI 269

Query: 245 GQPKRLKQAWEIIDQTIAKLLASKRESLKMMMIMKEES--DMITSYLTDYKHDDDKFLRD 304
           G PKRL++AWEIIDQTIA L+A+KRESL  +   K++   D+ITSY+ + K +DDK LRD
Sbjct: 270 GHPKRLQEAWEIIDQTIANLIATKRESLNQLREEKDQEGVDLITSYM-ENKENDDKMLRD 329

Query: 305 TILNITIAGRDTLSSALSWFFFCLSNNPTVEAKIREELKTAIPTNEGQNQQWIFPIEEVK 364
           T+LN  IAGRDTLSSALSWFFFCLSNNPTVEAKIREEL+T IPT+E + +  +F I+EV 
Sbjct: 330 TVLNFVIAGRDTLSSALSWFFFCLSNNPTVEAKIREELETTIPTSEPRGRWRMFSIKEVD 389

Query: 365 KLVYFHGALCETLRLYPPVPFQHKVPSQTDILPSGHYVNPKTKILFSLHALGRMSDVWGN 424
           KLVYFHGALCETLRLYPPVPFQHK+PSQ D+LPSGH+VNPKTKI+FSL+ALGRMSDVWG 
Sbjct: 390 KLVYFHGALCETLRLYPPVPFQHKIPSQPDVLPSGHHVNPKTKIIFSLYALGRMSDVWGK 449

Query: 425 DCMDFKPERWISERGSIKHVPSYKFLAFNAGPRTCLGKEVAFLEMKIVAAAIIHNYN-VE 484
           DCM+FKPERWISE G IKHVPSYKFLAFNAGPRTCLGK+VAF+EMKIVAAA+IHNY  V+
Sbjct: 450 DCMEFKPERWISETGMIKHVPSYKFLAFNAGPRTCLGKQVAFIEMKIVAAAMIHNYAIVQ 509

Query: 485 LIGHKVVPSASIILHMKHGFKIKISRRW 506
             G  VVPSASIILHMKHGFK+++++RW
Sbjct: 510 QTGQAVVPSASIILHMKHGFKVRVTKRW 535

BLAST of Sed0012335 vs. ExPASy TrEMBL
Match: A0A6J1IJU0 (alkane hydroxylase MAH1-like OS=Cucurbita maxima OX=3661 GN=LOC111478084 PE=3 SV=1)

HSP 1 Score: 775.0 bits (2000), Expect = 1.9e-220
Identity = 369/497 (74.25%), Postives = 430/497 (86.52%), Query Frame = 0

Query: 12  STLLPFLPFITFSIFFVFLKWNPRGIPWNWPFLGMLPTVALNIHRIHDRVTEILNQAGST 71
           S LL F+PF+ F++F + LKWN  G+PWNWPF GM PT+  +IHR+H RVT IL Q+ ST
Sbjct: 8   SILLAFIPFV-FALFVLSLKWNFNGLPWNWPFFGMTPTILFHIHRVHHRVTRILQQSPST 67

Query: 72  FFFKGLWFSGLDFFLTADPSNIHHILSTNFERYPKGPDFKYIFDVLGDGIFNSDSVSWQS 131
           F FKG WF+GLDFFLTADPSNIHHILSTNF+RYPKGPDFKYIFDVLGDGIFNSDSVSW++
Sbjct: 68  FLFKGAWFAGLDFFLTADPSNIHHILSTNFDRYPKGPDFKYIFDVLGDGIFNSDSVSWKT 127

Query: 132 QRRTAHALLRTHSFLQFSEEITIKKVKQGLIPILESVSEHGSVLDLQDLFQRFSFDSTCM 191
           QR TAHAL+R   FL F E+I +KKV++GL+ +LESV E+GSVLDLQDLFQRFSFDSTCM
Sbjct: 128 QRTTAHALVRQEKFLHFLEKIAMKKVREGLVSVLESVCENGSVLDLQDLFQRFSFDSTCM 187

Query: 192 LVTGFDLCSLSLEFPEIPFSKAMDDVEEVIFLRHFFPKKLWEFQSKFQIGQPKRLKQAWE 251
           LVTGFDL SLSLEFP++PFSKAMDD+EEVIFLRHF PKK+WEFQ K QIG P RL++AWE
Sbjct: 188 LVTGFDLHSLSLEFPQLPFSKAMDDIEEVIFLRHFIPKKIWEFQKKLQIGHPMRLQEAWE 247

Query: 252 IIDQTIAKLLASKRESLKMMMIMKEES--DMITSYLTDYKHDDDKFLRDTILNITIAGRD 311
           IIDQTIA L+A+KRESL  +   K++   D+ITSY+ + K +DDK LRDT+LN  IAGRD
Sbjct: 248 IIDQTIANLIATKRESLNQLKEEKDQEGVDLITSYM-ENKENDDKMLRDTVLNFMIAGRD 307

Query: 312 TLSSALSWFFFCLSNNPTVEAKIREELKTAIPTNEGQNQQWIFPIEEVKKLVYFHGALCE 371
           TLSSALSWFFFCLSNNPT EAKIREEL+T IP +E + Q  +F I+EV KLVYFHGALCE
Sbjct: 308 TLSSALSWFFFCLSNNPTAEAKIREELETTIPRSEPRGQWRMFSIKEVDKLVYFHGALCE 367

Query: 372 TLRLYPPVPFQHKVPSQTDILPSGHYVNPKTKILFSLHALGRMSDVWGNDCMDFKPERWI 431
           TLRLYPPVPFQHK+PSQ D+LPSG +VNPKTKI+FSL+ALGRMSDVWG DCM+FKPERWI
Sbjct: 368 TLRLYPPVPFQHKIPSQPDVLPSGLHVNPKTKIIFSLYALGRMSDVWGKDCMEFKPERWI 427

Query: 432 SERGSIKHVPSYKFLAFNAGPRTCLGKEVAFLEMKIVAAAIIHNYN-VELIGHKVVPSAS 491
           +E G IKHVPSYKF+AFNAGPRTC+GK+VAF EMKIVAAA+IHNY  V+  G +VVPSAS
Sbjct: 428 AETGRIKHVPSYKFVAFNAGPRTCIGKQVAFTEMKIVAAAMIHNYAIVQETGQEVVPSAS 487

Query: 492 IILHMKHGFKIKISRRW 506
           IILHMKHGFK+++++RW
Sbjct: 488 IILHMKHGFKVRVTKRW 502

BLAST of Sed0012335 vs. ExPASy TrEMBL
Match: A0A6J1BWK7 (alkane hydroxylase MAH1-like OS=Momordica charantia OX=3673 GN=LOC111005984 PE=3 SV=1)

HSP 1 Score: 760.8 bits (1963), Expect = 3.8e-216
Identity = 369/510 (72.35%), Postives = 428/510 (83.92%), Query Frame = 0

Query: 7   PPMAFSTLLPFLPFITFSIFFVFLKWNPRGIPWNWPFLGMLPTVALNIHRIHDRVTEILN 66
           P    S LLP LP I   IFF+ L WNP GIPWNWP LGM+PT+  N+HRIHDRVTEIL+
Sbjct: 3   PISILSILLPSLPLIFILIFFISLNWNPHGIPWNWPILGMIPTIFSNLHRIHDRVTEILH 62

Query: 67  QAGSTFFFKGLWFSGLDFFLTADPSNIHHILSTNFERYPKGPDFKYIFDVLGDGIFNSDS 126
           Q+ STFFFK +WF+ +DF LTADPSNIHHILS NF+RYPKGPDF YIFDVLGDGIFN+DS
Sbjct: 63  QSSSTFFFKAIWFANIDFLLTADPSNIHHILSANFQRYPKGPDFNYIFDVLGDGIFNADS 122

Query: 127 VSWQSQRRTAHALLRTHSFLQFSEEITIKKVKQGLIPILESVSEHGSVLDLQDLFQRFSF 186
            SW+ QR+ AH+L+    FL+F E+ T+KKVK+G++ +LESV E+GSVLDLQDLFQRFSF
Sbjct: 123 DSWKYQRKIAHSLVHRDEFLRFLEKNTMKKVKEGVVSVLESVCENGSVLDLQDLFQRFSF 182

Query: 187 DSTCMLVTGFDLCSLSLEFPEIPFSKAMDDVEEVIFLRHFFPKKLWEFQSKFQIGQPKRL 246
           DSTCMLVTGFDL SLSL+FP++PFSKAMDDVEE+IFLRHFFPK LWE  +K QIGQP+R 
Sbjct: 183 DSTCMLVTGFDLNSLSLDFPQVPFSKAMDDVEEIIFLRHFFPKALWELLNKLQIGQPRRQ 242

Query: 247 KQAWEIIDQTIAKLLASKRESLKMMMIMKEE----SDMITSYLTDYKHDD------DKFL 306
           KQA +IIDQTIA+L+A KRESL+     KEE    +D+ITSY+ D ++ D      + FL
Sbjct: 243 KQARQIIDQTIAELIALKRESLQ-----KEEAAQGADLITSYMIDEEYQDGNFEKNENFL 302

Query: 307 RDTILNITIAGRDTLSSALSWFFFCLSNNPTVEAKIREELKTAIPTNEGQNQQWIFPIEE 366
           RDT+LN  IAGRDTLSSALSWFFFCLSNNPTVEAKIREELK+ IP NE  +Q  IF IEE
Sbjct: 303 RDTVLNFMIAGRDTLSSALSWFFFCLSNNPTVEAKIREELKSIIPENEDHDQWRIFSIEE 362

Query: 367 VKKLVYFHGALCETLRLYPPVPFQHKVPSQTDILPSGHYVNPKTKILFSLHALGRMSDVW 426
           + KLVYFHGALCETLRLYPPVPFQHKV  + D+LPSG  V+P TKILFSL+ALGRMS+VW
Sbjct: 363 LNKLVYFHGALCETLRLYPPVPFQHKVXVEADVLPSGXKVDPTTKILFSLYALGRMSEVW 422

Query: 427 GNDCMDFKPERWISERGSIKHVPSYKFLAFNAGPRTCLGKEVAFLEMKIVAAAIIHNYN- 486
           G DC +FKPERWISE+GSIKHVPSYKFLAFNAGPRTCLGK+VAF E+K+VAAAIIHNYN 
Sbjct: 423 GKDCEEFKPERWISEKGSIKHVPSYKFLAFNAGPRTCLGKQVAFTEIKMVAAAIIHNYNI 482

Query: 487 VELIGHKVVPSASIILHMKHGFKIKISRRW 506
           ++ IGHKVVP+ SIILHMKHGFK+KI++RW
Sbjct: 483 IQEIGHKVVPNPSIILHMKHGFKVKITKRW 507

BLAST of Sed0012335 vs. ExPASy TrEMBL
Match: A0A0A0KJ78 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G487530 PE=3 SV=1)

HSP 1 Score: 760.4 bits (1962), Expect = 4.9e-216
Identity = 371/503 (73.76%), Postives = 425/503 (84.49%), Query Frame = 0

Query: 11  FSTLLPFLPFITFSIFFVFLKWNPRG-IPWNWPFLGMLPTVALNIHRIHDRVTEILNQAG 70
           F+ ++PFL FI  S  +   +W+  G IPWNWP +GM PTV  +IHR HDRVTE+L Q  
Sbjct: 11  FAFIIPFLVFIILSRIW---RWDFHGIIPWNWPIVGMTPTVIAHIHRPHDRVTEVLQQTA 70

Query: 71  STFFFKGLWFSGLDFFLTADPSNIHHILSTNFERYPKGPDFKYIFDVLGDGIFNSDSVSW 130
            TFFFKG+WFSG+DF  T DPSNIHHILS NFERYPKGPDFKYIF+VLGDGIFNSDS  W
Sbjct: 71  FTFFFKGIWFSGMDFLFTVDPSNIHHILSANFERYPKGPDFKYIFEVLGDGIFNSDSDVW 130

Query: 131 QSQRRTAHALLRTHSFLQFSEEITIKKVKQGLIPILESVSEHGSVLDLQDLFQRFSFDST 190
           ++QR+TAH L+R H+FLQF E+IT+ KVK GL+PIL+SV E+GSVLDLQDLFQRFSFDST
Sbjct: 131 KNQRKTAHYLVRHHNFLQFLEKITLNKVKTGLVPILDSVCENGSVLDLQDLFQRFSFDST 190

Query: 191 CMLVTGFDLCSLSLEFPEIPFSKAMDDVEEVIFLRHFFPKKLWEFQSKFQIGQPKRLKQA 250
           CM+VTGFDL SLSLEFPE+PFSKAMDD EEVIF+RHFFPK +WEFQ K QIGQPKRLKQA
Sbjct: 191 CMMVTGFDLNSLSLEFPEVPFSKAMDDAEEVIFVRHFFPKTIWEFQKKLQIGQPKRLKQA 250

Query: 251 WEIIDQTIAKLLASKRESLKMMMIM-----KEESDMITSYLTDYKHDDDKFLRDTILNIT 310
           WEIID+TIAKL+ASKR+SLK  M       KE  D+ITSY+T+ ++DD+ FLRDT+LN  
Sbjct: 251 WEIIDETIAKLIASKRKSLKNEMKEEDDNGKEGVDLITSYITNIENDDE-FLRDTVLNFM 310

Query: 311 IAGRDTLSSALSWFFFCLSNNPTVEAKIREELKTAIPTNEGQNQQWIFPIEEVKKLVYFH 370
           IAGRDTLSSALSWFFFCLSN+PTV  KIREEL+T IPTNE  +Q+ IF IEEV  LVYFH
Sbjct: 311 IAGRDTLSSALSWFFFCLSNHPTVVEKIREELRTTIPTNEAYDQRRIFSIEEVDNLVYFH 370

Query: 371 GALCETLRLYPPVPFQHKVPSQTDILPSGHYVNPKTKILFSLHALGRMSDVWGNDCMDFK 430
           G LCE LRLYPPVP QHKV  Q DILPSGH++ PKTKILFSL+ALGRMS+VWG DC++FK
Sbjct: 371 GTLCEALRLYPPVPLQHKVSIQHDILPSGHHIKPKTKILFSLYALGRMSEVWGKDCLEFK 430

Query: 431 PERWI-SERGSIKHVPSYKFLAFNAGPRTCLGKEVAFLEMKIVAAAIIHNYN-VELIGHK 490
           PERWI SE G IKHVPSYKFLAFNAGPRTCLGK VAF E+KIVAAAIIHNYN ++  GH+
Sbjct: 431 PERWINSENGKIKHVPSYKFLAFNAGPRTCLGKHVAFTELKIVAAAIIHNYNIIQQAGHE 490

Query: 491 VVPSASIILHMKHGFKIKISRRW 506
           VVPSASIILHMKHGFK+K+++RW
Sbjct: 491 VVPSASIILHMKHGFKVKVTKRW 509

BLAST of Sed0012335 vs. ExPASy TrEMBL
Match: A0A6J1BTU4 (alkane hydroxylase MAH1-like OS=Momordica charantia OX=3673 GN=LOC111005299 PE=3 SV=1)

HSP 1 Score: 759.2 bits (1959), Expect = 1.1e-215
Identity = 372/507 (73.37%), Postives = 424/507 (83.63%), Query Frame = 0

Query: 8   PMAFSTLLPFLPFITFSIFFVFLKWNPRG-IPWNWPFLGMLPTVALNIHRIHDRVTEILN 67
           P     L  F+PF+   IFF+ LKWN  G +PWNWP +GM PTVA +IHR HDR+TE+LN
Sbjct: 8   PENLFALFNFIPFL-LMIFFLILKWNFDGFVPWNWPLVGMTPTVACHIHRAHDRITEVLN 67

Query: 68  QAGSTFFFKGLWFSGLDFFLTADPSNIHHILSTNFERYPKGPDFKYIFDVLGDGIFNSDS 127
           Q G +F FKG+WF+ LDF LTADPSNIHHILS NFERYPKGPDFKYIFDVLGDGIFNSDS
Sbjct: 68  QTGCSFLFKGIWFTDLDFLLTADPSNIHHILSANFERYPKGPDFKYIFDVLGDGIFNSDS 127

Query: 128 VSWQSQRRTAHALLRTHSFLQFSEEITIKKVKQGLIPILESVSEHGSVLDLQDLFQRFSF 187
            SW+ QRR AH+L+R   FLQF EE T+KKVK+G++ +LESV E GSVLDLQDLFQRFSF
Sbjct: 128 DSWREQRRIAHSLVRHDKFLQFLEETTMKKVKEGVVLVLESVCEDGSVLDLQDLFQRFSF 187

Query: 188 DSTCMLVTGFDLCSLSLEFPEIPFSKAMDDVEEVIFLRHFFPKKLWEFQSKFQIGQPKRL 247
           DSTCMLVTGFD  SLS++FP++PFSKAMDDVEEVI LRHF PK LWE Q K QIGQPK+L
Sbjct: 188 DSTCMLVTGFDFNSLSIDFPQVPFSKAMDDVEEVILLRHFIPKSLWELQKKLQIGQPKKL 247

Query: 248 KQAWEIIDQTIAKLLASKRESLKMMMIMKEE----SDMITSYLT---DYKHDDDKFLRDT 307
           K+AWEIIDQTIAKL+A KRESL+     KEE    +D+ITSY+    D    ++ FLRDT
Sbjct: 248 KEAWEIIDQTIAKLIALKRESLQ-----KEEPAQGADLITSYMVNEEDGFEKNENFLRDT 307

Query: 308 ILNITIAGRDTLSSALSWFFFCLSNNPTVEAKIREELKTAIPTNEGQNQQWIFPIEEVKK 367
           +LN  IAGRDTLSSALSWFFFCLSNNPTVEAKIREELK+ IP NE  +Q  IF IEE+ K
Sbjct: 308 VLNFMIAGRDTLSSALSWFFFCLSNNPTVEAKIREELKSIIPENEDHDQWRIFSIEELNK 367

Query: 368 LVYFHGALCETLRLYPPVPFQHKVPSQTDILPSGHYVNPKTKILFSLHALGRMSDVWGND 427
           LVYFHGALCETLRLYPPVPFQHKV  + D+LPSGH V+P TKILFSL+ALGRMS+VWG D
Sbjct: 368 LVYFHGALCETLRLYPPVPFQHKVTVEADVLPSGHKVDPTTKILFSLYALGRMSEVWGKD 427

Query: 428 CMDFKPERWISERGSIKHVPSYKFLAFNAGPRTCLGKEVAFLEMKIVAAAIIHNYN-VEL 487
           C +FKPERWISE+GSIKHVPSYKFLAFNAGPRTCLGK+VAF E+K+VAAAIIHNYN ++ 
Sbjct: 428 CEEFKPERWISEKGSIKHVPSYKFLAFNAGPRTCLGKQVAFTEIKMVAAAIIHNYNIIQE 487

Query: 488 IGHKVVPSASIILHMKHGFKIKISRRW 506
           IGHKVVP+ SIILHMKHGFK+KI++RW
Sbjct: 488 IGHKVVPNPSIILHMKHGFKVKITKRW 508

BLAST of Sed0012335 vs. TAIR 10
Match: AT4G39490.1 (cytochrome P450, family 96, subfamily A, polypeptide 10 )

HSP 1 Score: 500.0 bits (1286), Expect = 2.3e-141
Identity = 244/511 (47.75%), Postives = 344/511 (67.32%), Query Frame = 0

Query: 11  FSTLLPFLPFITFSIFFVFLKWNPRGIPWNWPFLGMLPTVALNIHRIHDRVTEILNQAGS 70
           F   + F  F+ F  F +  K + R  P NWPF GM+P + + IHR++D +TEIL     
Sbjct: 7   FEISIAFFCFLLFRHFLINKKTH-RLCPTNWPFFGMIPGLLVEIHRVYDFITEILEVTNL 66

Query: 71  TFFFKGLWFSGLDFFLTADPSNIHHILSTNFERYPKGPDFKYIFDVLGDGIFNSDSVSWQ 130
           T+   G  F+ LD  +T DP+NIHHI+S+NF  YPKGP+FK +FD+LGDGIFN+DS  W+
Sbjct: 67  TYPCTGPCFANLDMLVTVDPANIHHIMSSNFANYPKGPEFKKLFDILGDGIFNADSELWK 126

Query: 131 SQRRTAHALLRTHSFLQFSEEITIKKVKQGLIPILESVSEHGSVLDLQDLFQRFSFDSTC 190
             R++A +++    F +FS   ++KK+++GL+P+L+ V++    +DLQD+FQRF+FD+T 
Sbjct: 127 DLRKSAQSMMMNPEFQKFSLATSLKKLEKGLVPLLDHVAKEKLAVDLQDMFQRFTFDTTF 186

Query: 191 MLVTGFDLCSLSLEFPEIPFSKAMDDVEEVIFLRHFFPKKLWEFQSKFQIGQPKRLKQAW 250
           +L TG+D   LS+E PE+ F++A+DD EE IF RH  P+  W  Q    +G  K++ +A 
Sbjct: 187 VLATGYDPGCLSVEMPEVEFARALDDAEEAIFFRHVKPEIFWRLQGLLGLGDEKKMTKAR 246

Query: 251 EIIDQTIAKLLASKRESLK--MMMIMKEESDMITSYL----TDYK---HDDDKFLRDTIL 310
             +D+  +K +A KR+ +      +     D++TSY+    T YK     D++FLRDTIL
Sbjct: 247 STLDRVCSKYIAIKRDEVSRGTNNVDSHSKDLLTSYMNLDTTKYKLLNPSDERFLRDTIL 306

Query: 311 NITIAGRDTLSSALSWFFFCLSNNPTVEAKIREELKTAI-------PTNEGQNQQWIFPI 370
              +AGRDT  S L+WFF+ L  NP V AKIR+E+ TA+             N    F  
Sbjct: 307 TFMLAGRDTTGSGLTWFFWLLIKNPEVIAKIRQEINTALFQRSKVDDDASNNNDSDSFSP 366

Query: 371 EEVKKLVYFHGALCETLRLYPPVPFQHKVPSQTDILPSGHYVNPKTKILFSLHALGRMSD 430
           +E+KKLVY HGA+CE+LRLYPPVPFQHK P++ D+LPSGH V+  +KILF L++LGRM  
Sbjct: 367 QELKKLVYLHGAICESLRLYPPVPFQHKSPTKPDVLPSGHKVDANSKILFCLYSLGRMKS 426

Query: 431 VWGNDCMDFKPERWISERGSIKHVPSYKFLAFNAGPRTCLGKEVAFLEMKIVAAAIIHNY 490
           VWG D ++FKPERWISE G+  H PSYKFL+FNAGPRTCLGKEVA ++MK VA  II NY
Sbjct: 427 VWGEDALEFKPERWISESGNSVHEPSYKFLSFNAGPRTCLGKEVAMMQMKSVAVKIIQNY 486

Query: 491 NVELI-GHKVVPSASIILHMKHGFKIKISRR 505
            ++++ G ++ P+ S+ILHMKHG K+ +++R
Sbjct: 487 EMKIVEGQQIEPAPSVILHMKHGLKVTVTKR 516

BLAST of Sed0012335 vs. TAIR 10
Match: AT4G39480.1 (cytochrome P450, family 96, subfamily A, polypeptide 9 )

HSP 1 Score: 497.3 bits (1279), Expect = 1.5e-140
Identity = 250/513 (48.73%), Postives = 343/513 (66.86%), Query Frame = 0

Query: 9   MAFSTLLPF-LPFITFSIFFVFL--KWNPRGIPWNWPFLGMLPTVALNIHRIHDRVTEIL 68
           MA  +LL   + F  F IF +FL  K   R    NWP LGMLP +   + R++D +TE+L
Sbjct: 1   MALVSLLEISIAFFCFLIFRIFLISKKPHRSFLTNWPLLGMLPGLLTVLPRVYDFITEVL 60

Query: 69  NQAGSTFFFKGLWFSGLDFFLTADPSNIHHILSTNFERYPKGPDFKYIFDVLGDGIFNSD 128
                 + F G +  G+D   T DP+NIHHI+S+NF  YPKG +FK +FDVLGDGIFN+D
Sbjct: 61  EDGNLNYLFIGPFLGGIDMLFTVDPANIHHIMSSNFANYPKGTEFKKLFDVLGDGIFNAD 120

Query: 129 SVSWQSQRRTAHALLRTHSFLQFSEEITIKKVKQGLIPILESVSEHGSVLDLQDLFQRFS 188
           S  W+  R+++ +++    F +FS   ++ K+++GL+P+L+ V++   V+DLQD+FQRF+
Sbjct: 121 SDLWKDLRKSSQSMMNHPDFQRFSLATSLSKLEKGLVPLLDHVAKEKLVVDLQDVFQRFT 180

Query: 189 FDSTCMLVTGFDLCSLSLEFPEIPFSKAMDDVEEVIFLRHFFPKKLWEFQSKFQIGQPKR 248
           FD+T +L TG+D   LS+E PEI F++A+DD EE IF RHF P+ +W+ Q    +G   +
Sbjct: 181 FDTTFVLATGYDPGCLSVEMPEIEFARALDDAEEAIFYRHFKPEVVWKMQRLIGVGAELK 240

Query: 249 LKQAWEIIDQTIAKLLASKRESLKMMMIMKEESDMITSYL----TDYK---HDDDKFLRD 308
           LK+A  I D+  +K +ASKR+ +   +      D++ S +    T YK     DD+FLRD
Sbjct: 241 LKRAHAIFDRVCSKCIASKRDEISQGIDSSSSKDLLMSSINVDTTKYKLLNPSDDRFLRD 300

Query: 309 TILNITIAGRDTLSSALSWFFFCLSNNPTVEAKIREELKTAI-PTNEGQN-----QQWIF 368
           TIL+  +AGRDT  SAL+WFF+ L NN     KIR+E+ T + P N+  +         F
Sbjct: 301 TILSFMLAGRDTTGSALTWFFWLLCNNQEAMTKIRQEINTNLFPRNKTDDGSVSYDSDSF 360

Query: 369 PIEEVKKLVYFHGALCETLRLYPPVPFQHKVPSQTDILPSGHYVNPKTKILFSLHALGRM 428
             +EVKKLVY HGA+CE LRLYPPVPF HK P++ D+LPSGH V   ++ILF L++LGRM
Sbjct: 361 NPQEVKKLVYLHGAVCEALRLYPPVPFNHKSPAKPDVLPSGHKVKANSRILFCLYSLGRM 420

Query: 429 SDVWGNDCMDFKPERWISERGSIKHVPSYKFLAFNAGPRTCLGKEVAFLEMKIVAAAIIH 488
             VWG D M+FKPERWISE G   H PSYKFL+FNAGPRTCLGKEVA  +MK VA  II 
Sbjct: 421 KSVWGEDAMEFKPERWISESGRSVHEPSYKFLSFNAGPRTCLGKEVAMTQMKTVAVKIIQ 480

Query: 489 NYNVELI-GHKVVPSASIILHMKHGFKIKISRR 505
           NY++ ++ GHK+ P+ S+ILHMKHG K+ +S+R
Sbjct: 481 NYDINVVEGHKIKPAPSVILHMKHGLKVTVSKR 513

BLAST of Sed0012335 vs. TAIR 10
Match: AT2G23180.1 (cytochrome P450, family 96, subfamily A, polypeptide 1 )

HSP 1 Score: 495.7 bits (1275), Expect = 4.3e-140
Identity = 254/516 (49.22%), Postives = 347/516 (67.25%), Query Frame = 0

Query: 9   MAFSTLLPF---LPFITFSI-FFVFLKWNPRGIPWNWPFLGMLPTVALNIHRIHDRVTEI 68
           MA  TLL     L F +F   +F+  K   R    NWPFLGMLP + + I R++D VTE+
Sbjct: 1   MALITLLEVSISLLFFSFLYGYFLISKKPHRSFLTNWPFLGMLPGLLVEIPRVYDFVTEL 60

Query: 69  LNQAGSTFFFKGLWFSGLDFFLTADPSNIHHILSTNFERYPKGPDFKYIFDVLGDGIFNS 128
           L  +  T+ FKG  F GLD  +T DP+NIHHI+S+NF  YPKG +FK IFDVLGDGIFN+
Sbjct: 61  LEASNLTYPFKGPCFGGLDMLITVDPANIHHIMSSNFANYPKGTEFKKIFDVLGDGIFNA 120

Query: 129 DSVSWQSQRRTAHALLRTHSFLQFSEEITIKKVKQGLIPILESVSEHGSVLDLQDLFQRF 188
           DS  W+  R++A +++    F +F+    + K+++GL+P+L+ V+E   V+DLQD+FQRF
Sbjct: 121 DSELWKDLRKSAQSMMTHQDFQRFTLRTIMSKLEKGLVPLLDYVAEKKQVVDLQDVFQRF 180

Query: 189 SFDSTCMLVTGFDLCSLSLEFPEIPFSKAMDDVEEVIFLRHFFPKKLWEFQSKFQIGQPK 248
           +FD++ +L TG D   LS E P+I F++A+D+ EE IF RH  P+ +W+ Q     G   
Sbjct: 181 TFDTSFVLATGVDPGCLSTEMPQIEFARALDEAEEAIFFRHVKPEIVWKMQRFIGFGDEL 240

Query: 249 RLKQAWEIIDQTIAKLLASKRESLK--MMMIMKEESDMITSYL--------TDYK---HD 308
           ++K+A    D+  +K +ASKR+ +   ++ I     D++  Y+        T YK     
Sbjct: 241 KMKKAHSTFDRVCSKCIASKRDEITNGVINIDSSSKDLLMCYMNVDTICHTTKYKLLNPS 300

Query: 309 DDKFLRDTILNITIAGRDTLSSALSWFFFCLSNNPTVEAKIREELKTAIP--TNEGQNQQ 368
           DDKFLRD IL+  +AGRDT SSAL+WFF+ LS NP    KIR+E+ T +   TN+  +  
Sbjct: 301 DDKFLRDMILSFMLAGRDTTSSALTWFFWLLSKNPKAITKIRQEINTQLSPRTNDFDS-- 360

Query: 369 WIFPIEEVKKLVYFHGALCETLRLYPPVPFQHKVPSQTDILPSGHYVNPKTKILFSLHAL 428
             F  +E+ KLVY HGALCE LRLYPPVPFQHK P+++D+LPSGH V+  +KI+F L++L
Sbjct: 361 --FNAQELNKLVYVHGALCEALRLYPPVPFQHKSPTKSDVLPSGHRVDASSKIVFCLYSL 420

Query: 429 GRMSDVWGNDCMDFKPERWISERGSIKHVPSYKFLAFNAGPRTCLGKEVAFLEMKIVAAA 488
           GRM  VWG D  +FKPERWISE G + HVPS+KFL+FNAGPRTCLGKEVA  +MK VA  
Sbjct: 421 GRMKSVWGEDASEFKPERWISESGRLIHVPSFKFLSFNAGPRTCLGKEVAMTQMKTVAVK 480

Query: 489 IIHNYNVELI-GHKVVPSASIILHMKHGFKIKISRR 505
           II NY ++++ GHK+ P  SIILHMKHG K+ +++R
Sbjct: 481 IIQNYEIKVVEGHKIEPVPSIILHMKHGLKVTVTKR 512

BLAST of Sed0012335 vs. TAIR 10
Match: AT1G65340.1 (cytochrome P450, family 96, subfamily A, polypeptide 3 )

HSP 1 Score: 481.5 bits (1238), Expect = 8.5e-136
Identity = 240/508 (47.24%), Postives = 332/508 (65.35%), Query Frame = 0

Query: 14  LLPFLPFITFSIFFVFL------KWNPRGIPWNWPFLGMLPTVALNIHRIHDRVTEILNQ 73
           ++  L   T  +FF+F       K  P+ +  NWP LGMLP + L + RI+D +TE+L  
Sbjct: 4   VIGLLEIFTAFVFFIFFQCLLLHKKTPKPLLTNWPALGMLPGLLLQVPRIYDWITEVLEA 63

Query: 74  AGSTFFFKGLWFSGLDFFLTADPSNIHHILSTNFERYPKGPDFKYIFDVLGDGIFNSDSV 133
              TF FKG   SG+D  LT DP NIH+ILS+NF  YPKG +FK IF+V+GD IFN DS 
Sbjct: 64  TDMTFCFKGPCLSGMDILLTVDPVNIHYILSSNFANYPKGMEFKKIFEVVGDSIFNVDSG 123

Query: 134 SWQSQRRTAHALLRTHSFLQFSEEITIKKVKQGLIPILESVSEHGSVLDLQDLFQRFSFD 193
            W+  R ++HA+     F  F    +++K++QGL+PILE+ ++   ++DLQDLFQRF FD
Sbjct: 124 LWEDMRNSSHAIFSNQDFQMFWVSTSVRKLRQGLVPILENAADKNILVDLQDLFQRFLFD 183

Query: 194 STCMLVTGFDLCSLSLEFPEIPFSKAMDDVEEVIFLRHFFPKKLWEFQSKFQIGQPKRLK 253
           ++ +L+TG+D   LS+E P++ F  A+D V + +F RH  P  LW  Q    +G  KRLK
Sbjct: 184 TSLILMTGYDPKCLSVEMPKVEFGDAVDGVSDGVFYRHVKPVFLWRLQYLIGVGVEKRLK 243

Query: 254 QAWEIIDQTIAKLLASKRESLKM----MMIMKEESDMITSYL----TDYKH---DDDKFL 313
           +   + DQ + K++ +KRE +           E  D++T Y+    T YK+    DD+F+
Sbjct: 244 RGLAVFDQLLEKIITAKREEINSHGTHHPSRGEAIDVLTYYMTMDTTKYKYLEPSDDRFI 303

Query: 314 RDTILNITIAGRDTLSSALSWFFFCLSNNPTVEAKIREELKTAIPTNEGQNQQWIFPIEE 373
           +DTIL   IA RDT SSAL+WFF+ +S NP    KIR+E+   +P          F   +
Sbjct: 304 KDTILGFLIAARDTTSSALTWFFWLMSKNPEAINKIRQEVNKKMPR---------FDPAD 363

Query: 374 VKKLVYFHGALCETLRLYPPVPFQHKVPSQTDILPSGHYVNPKTKILFSLHALGRMSDVW 433
           ++KLVY HGA+CETLRLYPPVPF HK P++ D+LPSGH V+ K KI+ S++ALGRM  VW
Sbjct: 364 LEKLVYLHGAVCETLRLYPPVPFNHKSPAKPDVLPSGHRVDEKWKIVISMYALGRMKSVW 423

Query: 434 GNDCMDFKPERWISERGSIKHVPSYKFLAFNAGPRTCLGKEVAFLEMKIVAAAIIHNYNV 493
           G+D  DF+PERWIS+ G +KH PSYKFLAFNAGPR CLGK++ FL+MK VAA II NY++
Sbjct: 424 GDDAEDFRPERWISDSGRLKHEPSYKFLAFNAGPRACLGKKLTFLQMKTVAAEIIRNYDI 483

Query: 494 ELI-GHKVVPSASIILHMKHGFKIKISR 504
           +++ GHK  P  S++  M+HG K+ I+R
Sbjct: 484 KVVEGHKTEPVPSVLFRMQHGLKVNITR 502

BLAST of Sed0012335 vs. TAIR 10
Match: AT2G21910.1 (cytochrome P450, family 96, subfamily A, polypeptide 5 )

HSP 1 Score: 473.0 bits (1216), Expect = 3.0e-133
Identity = 236/502 (47.01%), Postives = 334/502 (66.53%), Query Frame = 0

Query: 17  FLPFITFSIF-FVFLKWNPRGIPWNWPFLGMLPTVALNIHRIHDRVTEILNQAGSTFFFK 76
           F+  + F  F F+  K + +  P NWP LGMLP V + +HRI+D V EIL  +  TF FK
Sbjct: 10  FIALLVFFFFHFLIHKKSHQITPRNWPVLGMLPGVLVMLHRINDYVAEILEVSNLTFAFK 69

Query: 77  GLWFSGLDFFLTADPSNIHHILSTNFERYPKGPDFKYIFDVLGDGIFNSDSVSWQSQRRT 136
           G WFSG++  +TADPSNI H+ S+NF  Y KGP+FK +FD LG+GIF +DS  W+  R++
Sbjct: 70  GPWFSGMNMLITADPSNIQHVFSSNFSNYDKGPEFKEMFDFLGNGIFTADSKLWEDMRKS 129

Query: 137 AHALLRTHSFLQFSEEITIKKVKQGLIPILESVSEHGSVLDLQDLFQRFSFDSTCMLVTG 196
           A  +L    F  FS      K+K GL+P+L+  +E  +V DLQD+FQR +FD T  LVTG
Sbjct: 130 ALVVLSHQGFQSFSLRTITCKIKNGLVPVLDHFAEANTVFDLQDVFQRLAFDVTLTLVTG 189

Query: 197 FDLCSLSLEFPEIPFSKAMDDVEEVIFLRHFFPKKLWEFQSKFQIGQPKRLKQAWEIIDQ 256
            D  SLS+E P+  ++KAMDD EEV+  RH  P  LW+ Q+   +G+ K++K+A    D+
Sbjct: 190 CDSSSLSIEMPKNEYAKAMDDAEEVVVYRHVKPVVLWKLQNWIGLGEEKKMKEANAAFDR 249

Query: 257 TIAKLLASKRESL----KMMMIMKEESDMITSY----LTDYK---HDDDKFLRDTILNIT 316
           + AK +++KRE +      +       D+++ Y    ++ Y+    +DD FL+D I +  
Sbjct: 250 SCAKYISAKREEIISHHSNIGGEAHAEDLLSVYMNLDISKYELLNPNDDNFLKDIIKSFM 309

Query: 317 IAGRDTLSSALSWFFFCLSNNPTVEAKIREELKTAIPTNEGQNQQWIFPIEEVKKLVYFH 376
           +AGRD +++ L+WFF+ LS NP    KIR+E+ T +P   G +       +++ K+VY H
Sbjct: 310 LAGRDAIATTLTWFFWLLSKNPEAVTKIRQEINTNLP-GSGMS----LDADKLNKMVYLH 369

Query: 377 GALCETLRLYPPVPFQHKVPSQTDILPSGHYVNPKTKILFSLHALGRMSDVWGNDCMDFK 436
           GALCE+LRLY P+PF+ K P + D+LPSGH V+   KILFS++ALGRM  VWG D  +FK
Sbjct: 370 GALCESLRLYAPIPFERKTPIKQDVLPSGHMVDKNWKILFSVYALGRMRSVWGQDASEFK 429

Query: 437 PERWISER-GSIKHVPSYKFLAFNAGPRTCLGKEVAFLEMKIVAAAIIHNYNVELI-GHK 496
           PERWISER G +KH PS+KF  FN+GPR CLGK ++FL+MK VA  II NY+++++ GHK
Sbjct: 430 PERWISERNGGLKHEPSFKFFVFNSGPRNCLGKNLSFLQMKTVAVEIIRNYDIKVVEGHK 489

Query: 497 VVPSASIILHMKHGFKIKISRR 505
           + P++SIILHMKHG K+ +S+R
Sbjct: 490 IEPASSIILHMKHGLKVTVSKR 506

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022950281.13.6e-22173.43alkane hydroxylase MAH1-like [Cucurbita moschata][more]
XP_022977942.14.0e-22074.25alkane hydroxylase MAH1-like [Cucurbita maxima][more]
KAG7034398.15.2e-22074.25Alkane hydroxylase MAH1, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_023544417.11.2e-21974.04alkane hydroxylase MAH1-like [Cucurbita pepo subsp. pepo][more]
XP_038882620.16.3e-21875.10alkane hydroxylase MAH1-like isoform X1 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
Q9FVS91.4e-13046.09Alkane hydroxylase MAH1 OS=Arabidopsis thaliana OX=3702 GN=CYP96A15 PE=2 SV=1[more]
A0A140IL903.2e-11139.64Noroxomaritidine synthase OS=Narcissus aff. pseudonarcissus MK-2014 OX=1540222 G... [more]
A0A140IL925.4e-11139.64Noroxomaritidine synthase 3 OS=Narcissus aff. pseudonarcissus MK-2014 OX=1540222... [more]
A0A140IL911.2e-11039.64Noroxomaritidine synthase 2 OS=Narcissus aff. pseudonarcissus MK-2014 OX=1540222... [more]
A0A2H5AJ003.5e-11039.44Noroxomaritidine synthase 1 OS=Narcissus pseudonarcissus OX=39639 GN=Cyp96T1 PE=... [more]
Match NameE-valueIdentityDescription
A0A6J1GEE61.7e-22173.43alkane hydroxylase MAH1-like OS=Cucurbita moschata OX=3662 GN=LOC111453415 PE=3 ... [more]
A0A6J1IJU01.9e-22074.25alkane hydroxylase MAH1-like OS=Cucurbita maxima OX=3661 GN=LOC111478084 PE=3 SV... [more]
A0A6J1BWK73.8e-21672.35alkane hydroxylase MAH1-like OS=Momordica charantia OX=3673 GN=LOC111005984 PE=3... [more]
A0A0A0KJ784.9e-21673.76Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G487530 PE=3 SV=1[more]
A0A6J1BTU41.1e-21573.37alkane hydroxylase MAH1-like OS=Momordica charantia OX=3673 GN=LOC111005299 PE=3... [more]
Match NameE-valueIdentityDescription
AT4G39490.12.3e-14147.75cytochrome P450, family 96, subfamily A, polypeptide 10 [more]
AT4G39480.11.5e-14048.73cytochrome P450, family 96, subfamily A, polypeptide 9 [more]
AT2G23180.14.3e-14049.22cytochrome P450, family 96, subfamily A, polypeptide 1 [more]
AT1G65340.18.5e-13647.24cytochrome P450, family 96, subfamily A, polypeptide 3 [more]
AT2G21910.13.0e-13347.01cytochrome P450, family 96, subfamily A, polypeptide 5 [more]
InterPro
Analysis Name: InterPro Annotations of Chayote (edule) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 453..476
score: 23.76
coord: 295..312
score: 30.83
coord: 315..341
score: 30.64
coord: 443..453
score: 47.71
IPR001128Cytochrome P450PRINTSPR00385P450coord: 306..323
score: 38.09
coord: 444..453
score: 51.2
coord: 365..376
score: 38.96
coord: 453..464
score: 39.82
IPR001128Cytochrome P450PFAMPF00067p450coord: 36..485
e-value: 6.0E-66
score: 223.1
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 26..506
e-value: 5.2E-93
score: 314.2
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 29..504
NoneNo IPR availablePANTHERPTHR24296CYTOCHROME P450coord: 19..504
NoneNo IPR availablePANTHERPTHR24296:SF193ALKANE HYDROXYLASE MAH1-LIKEcoord: 19..504
IPR017972Cytochrome P450, conserved sitePROSITEPS00086CYTOCHROME_P450coord: 446..455

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sed0012335.1Sed0012335.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen