Sed0011553 (gene) Chayote v1

BLAST of Sed0011553 vs. NCBI nr
Match: XP_022995763.1 (E4 SUMO-protein ligase PIAL2-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 1230.7 bits (3183), Expect = 0.0e+00
Identity = 655/859 (76.25%), Postives = 713/859 (83.00%), Query Frame = 0

Query: 1   MGAPSPYDLTMNRISLYIDGLTSHVNRIDQIDPAHFCSLCFSLARCIDFAIASNFVPSKV 60
           MG P PY++  NRISLYIDG+TSHVNR DQIDPA+FC+LCFSLARCIDFAIA+NFVPS V
Sbjct: 1   MGTPLPYEMYSNRISLYIDGITSHVNRYDQIDPAYFCNLCFSLARCIDFAIANNFVPSNV 60

Query: 61  HGLPTLLKQMCQKKHSHSHHLKAAIVVVLVSTKSACKARWFSEKEAEELYSLANEIGSDF 120
           HGLP LLKQM QKK  HSH LKAA++V+++STK+ACK RWFSEKEAE+LYSLANEIGSDF
Sbjct: 61  HGLPNLLKQMYQKK--HSHRLKAAVMVLMISTKNACKVRWFSEKEAEDLYSLANEIGSDF 120

Query: 121 FGDSNIGQSNSLTAITTVMERFFPHLKLGQIVASMEVKPGYGVFTIHFNISKTMQYTPQE 180
           FGD+N G +NSLT IT VMERFFPHLKLGQIVA+MEVKPGYGVF   FNISKTMQ++ Q+
Sbjct: 121 FGDTNTGPNNSLTTITAVMERFFPHLKLGQIVAAMEVKPGYGVFATDFNISKTMQFSQQD 180

Query: 181 KIRLFVAQKDNTETSACIVSPPQVNFLVNGIGVNGRTISYMDTGPQLPTNVTHLLKLGSN 240
           KI LFVAQKDNTETSACI+SPPQVNFLVNG GVNGRT  +MDTGPQLPTNVTH+LKLG+N
Sbjct: 181 KILLFVAQKDNTETSACIISPPQVNFLVNGKGVNGRTNIFMDTGPQLPTNVTHMLKLGAN 240

Query: 241 LFQAIGSFNGHYVIAVAIMGTAPLPDSSVLQDHVQPVVSTVDSDSDIIEGPSRISLNCPI 300
           L QAIG+FNGHYVIAVAIMGTAPLPDSSVLQD+VQPVVSTVDSDSDIIEGPSRISLNCPI
Sbjct: 241 LLQAIGNFNGHYVIAVAIMGTAPLPDSSVLQDYVQPVVSTVDSDSDIIEGPSRISLNCPI 300

Query: 301 SYTRIKVPVKGRSCKHLQCFDFYNFIDINSRRPSWRCPHCNQYICFLDIRVDQNMLKVIK 360
           SYTRIKVPVK RSCKHLQCFDFYNFI INSRRPSWRCPHCNQYICFLDIRVDQNM+KVI+
Sbjct: 301 SYTRIKVPVKSRSCKHLQCFDFYNFIGINSRRPSWRCPHCNQYICFLDIRVDQNMMKVIR 360

Query: 361 EVAENVTEVIISADGSWKAILENDDRNGQSLDDSLNHQNEMEQQESTTAPDVVDLTQVDD 420
           EVAENVTEVIISADGSWKAILEND+ +G+ LDDSLN QNE +Q+  +T PDV+DL +VDD
Sbjct: 361 EVAENVTEVIISADGSWKAILENDNGDGRPLDDSLNLQNERDQE--STVPDVLDLIEVDD 420

Query: 421 DMDICDLEIEDTKPCLGNQNQLLSSGLNTPSEMNSNSSNPNFAAVLDDDFWSGIETGEIL 480
           D++ICDLEIED KPCLGN+                     NFAAVLDDDFWSGI+T  IL
Sbjct: 421 DINICDLEIEDEKPCLGNK---------------------NFAAVLDDDFWSGIDTDRIL 480

Query: 481 TSSTRSD--------APNFTGLMQLAGLTDPAIPAL-NHVVGVPGPVTFSSPALYDQNNL 540
           TSS R+D        APNF GLMQ A LT+P  P L NH  GVPG V F SPALYDQNNL
Sbjct: 481 TSSARTDAPIGNNPPAPNFAGLMQSAVLTNPVTPVLNNHGAGVPGHVIFLSPALYDQNNL 540

Query: 541 QIHVLTSNDNNQYGRMASMVRPSFTVSSRTPTAVQALPAQSQASGQPYGSRTPTISSAPQ 600
           Q   L SN+N +YGR  S+ RP     SR PT  QALP  SQASGQ Y SRT TISSA Q
Sbjct: 541 QTQALNSNENTEYGRTTSIARP----LSRMPTTAQALPYPSQASGQQYSSRTTTISSASQ 600

Query: 601 VGQNIPINRDGLNTISLDSERRQQLPRHPGDSPHATNPSPFHHPQTMQNRDPQDRSFTHG 660
           VG +IP NRDGLNTIS DSER QQ PRHPGDS HATN +PFHHP T QNRDP   SFT G
Sbjct: 601 VGPSIPTNRDGLNTISRDSERSQQFPRHPGDSHHATNLAPFHHPPTSQNRDP--HSFTPG 660

Query: 661 QSVQASTALRPSLGILTDFQNPHLQQALNFRMSNFRNPIPS-NARPSSPFSRPMSQV-SG 720
           QSVQASTALRPS  +LTDFQNPHLQQALN RMS  RN  PS N RPS PFSR MSQV  G
Sbjct: 661 QSVQASTALRPSTRLLTDFQNPHLQQALNLRMSQLRNQNPSNNVRPSLPFSRAMSQVGGG 720

Query: 721 YGGSAYAAVT--SQHARMMAISQRAELMRQSSAMPLQNQTPRSAHSLQTTPDGLGI-SAG 780
           Y G +YAAVT  SQ+ARM+A SQRAELMRQSSAM LQNQT RSAHSLQTTPDGL + +AG
Sbjct: 721 YSGPSYAAVTPNSQNARMVA-SQRAELMRQSSAMSLQNQTFRSAHSLQTTPDGLRMPAAG 780

Query: 781 DLRHVGGMSQSVTTAAGSVDLTSEQNWQPSGRMRGSLSGRAYSDAYGHLIIQPTQSSQSA 840
           +LR+VGGMSQSVT AAG VD +SEQNWQPSGRMRGSLSGRA+SDA+GHLII PTQS QSA
Sbjct: 781 ELRNVGGMSQSVTLAAGLVDPSSEQNWQPSGRMRGSLSGRAFSDAHGHLIIHPTQSVQSA 827

Query: 841 QPPSNSTPTQPGALFTQAQ 846
           +PPSN TPTQP A  TQAQ
Sbjct: 841 RPPSNPTPTQPSAPSTQAQ 827

BLAST of Sed0011553 vs. NCBI nr
Match: KAG6579533.1 (E4 SUMO-protein ligase PIAL2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1224.9 bits (3168), Expect = 0.0e+00
Identity = 645/856 (75.35%), Postives = 708/856 (82.71%), Query Frame = 0

Query: 1   MGAPSPYDLTMNRISLYIDGLTSHVNRIDQIDPAHFCSLCFSLARCIDFAIASNFVPSKV 60
           MGA +PY++ ++RIS YID LT +VNR+DQIDP   C++CFSLAR IDFAIA++FVPSK 
Sbjct: 1   MGATTPYEMKLDRISSYIDSLTLYVNRVDQIDPVQLCNICFSLARSIDFAIANDFVPSKA 60

Query: 61  HGLPTLLKQMCQKKHSHSHHLKAAIVVVLVSTKSACKARWFSEKEAEELYSLANEIGSDF 120
            GLP+LLKQ+CQKK  HSHHLKAAI+V++++ K+ACK +WFSEKEAEELYSLANEIGSDF
Sbjct: 61  QGLPSLLKQICQKK--HSHHLKAAIMVLMIAAKNACKVKWFSEKEAEELYSLANEIGSDF 120

Query: 121 FGDSNIGQSNSLTAITTVMERFFPHLKLGQIVASMEVKPGYGVFTIHFNISKTMQYTPQE 180
           F D+N G SNSLT ITTVMERFFP LKLGQIV S EVKPGYGVF   FNISKT+QY PQE
Sbjct: 121 FVDTNTGPSNSLTTITTVMERFFPRLKLGQIVISAEVKPGYGVFAFDFNISKTIQYAPQE 180

Query: 181 KIRLFVAQKDNTETSACIVSPPQVNFLVNGIGVNGRTISYMDTGPQLPTNVTHLLKLGSN 240
           KIRLFVAQKDNTETSACI+SPPQVNFLVNG GVNGRT  YMDTGPQLPTNVTH+LKLGSN
Sbjct: 181 KIRLFVAQKDNTETSACIISPPQVNFLVNGRGVNGRTNIYMDTGPQLPTNVTHMLKLGSN 240

Query: 241 LFQAIGSFNGHYVIAVAIMGTAPLPDSSVLQDHVQPVVSTVDSDSDIIEGPSRISLNCPI 300
           L Q IGSFNGHYVIAVA+MG+AP PDSSVLQDH QPVVSTVDSDSDIIEGPSRISLNCPI
Sbjct: 241 LLQVIGSFNGHYVIAVAVMGSAPSPDSSVLQDHEQPVVSTVDSDSDIIEGPSRISLNCPI 300

Query: 301 SYTRIKVPVKGRSCKHLQCFDFYNFIDINSRRPSWRCPHCNQYICFLDIRVDQNMLKVIK 360
           SYTRIKVPVKGRSCKHLQCFDFYNFIDINSRRPSWRCPHCNQYICFLDI +DQNMLKVI+
Sbjct: 301 SYTRIKVPVKGRSCKHLQCFDFYNFIDINSRRPSWRCPHCNQYICFLDICIDQNMLKVIR 360

Query: 361 EVAENVTEVIISADGSWKAILENDDRNGQSLDDSLNHQNEMEQQESTTAPDVVDLTQVDD 420
           EVAENVTEVIISADGSWKAILEND  +G+ LDDSLN QNE  QQEST  PDV+DLT+VDD
Sbjct: 361 EVAENVTEVIISADGSWKAILENDCGDGRPLDDSLNQQNERAQQESTAPPDVLDLTEVDD 420

Query: 421 DMDICDLEIEDTKPCLGNQNQLLSSGLNTPSEMNSNSSNPNFAAVLDDDFWSGIETGEIL 480
           DM+IC+LE ED KPCLGN+NQ +SS LN  S MN NS N NF+A LDDDFWSG+ T  +L
Sbjct: 421 DMNICNLETEDRKPCLGNKNQPVSSSLNILSGMNRNSLNQNFSAALDDDFWSGMVTDRLL 480

Query: 481 TSSTRSD--------APNFTGLMQLAGLTDPAIPALNHVVGVPGPVTFSSPALYDQNNLQ 540
           TSS RSD        AP+F GL Q AGLTD   P LNH VGVPG V F  PA YDQNN+Q
Sbjct: 481 TSSIRSDAPMGSSTAAPSFAGLTQSAGLTDAVSPVLNHDVGVPGQVNFPFPAFYDQNNVQ 540

Query: 541 IHVLTSNDNNQYGRMASMVRPSFTVSSRTPTAVQALPAQSQASGQPYGSRTPTISSAPQV 600
           + V  SN++NQYGRM S+ RP     SRT  A Q LPAQSQ SGQ Y SRT TISSAPQV
Sbjct: 541 VQVSNSNESNQYGRMTSIARP----VSRT-LAGQVLPAQSQTSGQQYSSRTSTISSAPQV 600

Query: 601 GQNIPINRDGLNTISLDSERRQQLPRHPGDSPHATNPSPFHHPQTMQNRDPQDRSFTHGQ 660
           GQ+IPI+RDGLNTIS DSERRQ  PRH GD  HATN +PF  P  +QNR+PQDRSFT GQ
Sbjct: 601 GQSIPISRDGLNTISRDSERRQPFPRHHGDLHHATNLAPFLRPPIVQNREPQDRSFTPGQ 660

Query: 661 SVQASTALRPSLGILTDFQNPHLQQALNFRMSNFRNPIPSNARPSSPFSRPMSQV-SGYG 720
           SV+ASTA RPS GILTDFQNPHLQQALN R+S+ RN  PS+ RPS PFSRP SQV  GYG
Sbjct: 661 SVRASTAQRPSAGILTDFQNPHLQQALNLRISHLRNQNPSSVRPSLPFSRPTSQVGGGYG 720

Query: 721 GSAYAAVT--SQHARMMAISQRAELMRQSSAMPLQNQTPRSAHSLQTTPDGLGISAGDLR 780
           GSAY AVT  +QHARMM  SQRAE+MRQSSAM LQNQT RS H LQTTPDGL   AGDLR
Sbjct: 721 GSAYPAVTPHNQHARMMVASQRAEMMRQSSAMSLQNQTSRSPHPLQTTPDGLRRPAGDLR 780

Query: 781 HVGGMSQSVTTAAGSVDLTSEQNWQPSGRMRGSLSGRAYSDAYGHLIIQPTQSSQSAQPP 840
           +VGGM+QSVT A+  +D + EQN QP GRMRGSLSGRAYSDAYG +IIQPTQ  QSA+PP
Sbjct: 781 NVGGMTQSVTMASDLLDPSVEQNRQPIGRMRGSLSGRAYSDAYG-VIIQPTQPVQSARPP 840

Query: 841 SNSTPTQPGALFTQAQ 846
           SN T TQ  A  T AQ
Sbjct: 841 SNLTTTQSSAPSTHAQ 848

BLAST of Sed0011553 vs. NCBI nr
Match: XP_022958575.1 (E4 SUMO-protein ligase PIAL2-like isoform X4 [Cucurbita moschata])

HSP 1 Score: 1223.8 bits (3165), Expect = 0.0e+00
Identity = 652/858 (75.99%), Postives = 710/858 (82.75%), Query Frame = 0

Query: 1   MGAPSPYDLTMNRISLYIDGLTSHVNRIDQIDPAHFCSLCFSLARCIDFAIASNFVPSKV 60
           MG P PY++ +NRISLYIDG+TS VNR DQIDPA+FC+LCFSLARCIDFAIA+NFVPS V
Sbjct: 1   MGTPLPYEMYLNRISLYIDGITSLVNRNDQIDPAYFCNLCFSLARCIDFAIANNFVPSNV 60

Query: 61  HGLPTLLKQMCQKKHSHSHHLKAAIVVVLVSTKSACKARWFSEKEAEELYSLANEIGSDF 120
           HGLP+LLKQM QKK  HSH LKAA++V+++STK+ACK RWFSEKEAEELYSLANEIGSDF
Sbjct: 61  HGLPSLLKQMYQKK--HSHRLKAAVMVLMISTKNACKVRWFSEKEAEELYSLANEIGSDF 120

Query: 121 FGDSNIGQSNSLTAITTVMERFFPHLKLGQIVASMEVKPGYGVFTIHFNISKTMQYTPQE 180
           FGD+N G +NSLT IT VMERFFPHLKLGQIVA MEVKPGYGVF   FNISKTMQ + QE
Sbjct: 121 FGDTNTGPNNSLTTITAVMERFFPHLKLGQIVADMEVKPGYGVFATDFNISKTMQLSQQE 180

Query: 181 KIRLFVAQKDNTETSACIVSPPQVNFLVNGIGVNGRTISYMDTGPQLPTNVTHLLKLGSN 240
           KI LFVAQKDNTETSACI+SPPQVNFLVNG GVNGRT  +MDTGPQLPTNVTH+LKLG+N
Sbjct: 181 KILLFVAQKDNTETSACIISPPQVNFLVNGKGVNGRTNIFMDTGPQLPTNVTHMLKLGAN 240

Query: 241 LFQAIGSFNGHYVIAVAIMGTAPLPDSSVLQDHVQPVVSTVDSDSDIIEGPSRISLNCPI 300
           L QAIG+FNGHYVIAVAIMGTAPLPDSSVLQD+VQ VVSTVD DSDIIEGPSRISLNCPI
Sbjct: 241 LLQAIGNFNGHYVIAVAIMGTAPLPDSSVLQDYVQTVVSTVDPDSDIIEGPSRISLNCPI 300

Query: 301 SYTRIKVPVKGRSCKHLQCFDFYNFIDINSRRPSWRCPHCNQYICFLDIRVDQNMLKVIK 360
           SYTRIKVPVK RSCKHLQCFDFYNFI INSRRPSWRCPHCNQYICFLDIRVDQNMLKVI+
Sbjct: 301 SYTRIKVPVKSRSCKHLQCFDFYNFIGINSRRPSWRCPHCNQYICFLDIRVDQNMLKVIR 360

Query: 361 EVAENVTEVIISADGSWKAILENDDRNGQSLDDSLNHQNEMEQQESTTAPDVVDLTQVDD 420
           EVAENVTEVIISADGSWKAIL+N D  G+ LDDSLN QNE +Q+  +T P V+DL +VDD
Sbjct: 361 EVAENVTEVIISADGSWKAILDNGD--GRPLDDSLNLQNERDQE--STVPVVLDLIEVDD 420

Query: 421 DMDICDLEIEDTKPCLGNQNQLLSSGLNTPSEMNSNSSNPNFAAVLDDDFWSGIETGEIL 480
           D++ICDLEIED KPC+GN+                     NFAAVLDDDFWSGI+T  IL
Sbjct: 421 DINICDLEIEDEKPCIGNK---------------------NFAAVLDDDFWSGIDTDRIL 480

Query: 481 TSSTRSD--------APNFTGLMQLAGLTDPAIPAL-NHVVGVPGPVTFSSPALYDQNNL 540
           TSS R+D        APNF GLMQ A LT+P  P L NH  GVPG V FSSPALYDQNNL
Sbjct: 481 TSSARTDAPIGNNPPAPNFAGLMQSAVLTNPVTPVLNNHGAGVPGHVIFSSPALYDQNNL 540

Query: 541 QIHVLTSNDNNQYGRMASMVRPSFTVSSRTPTAVQALPAQSQASGQPYGSRTPTISSAPQ 600
           Q   L SN+N++YGR  S+ RP     SR PT  QALP+ SQASGQ Y SRT TISSAPQ
Sbjct: 541 QSQALNSNENSEYGRTTSIARP----LSRMPTTAQALPSLSQASGQQYSSRTTTISSAPQ 600

Query: 601 VGQNIPINRDGLNTISLDSERRQQLPRHPGDSPHATNPSPFHHPQTMQNRDPQDRSFTHG 660
           VG +IP NRDGLNTIS DSER QQ PR PGDS HATN +PFHHP T+QN DP D SFT G
Sbjct: 601 VGPSIPTNRDGLNTISRDSERSQQFPRRPGDSHHATNLAPFHHPPTLQNWDPLDHSFTPG 660

Query: 661 QSVQASTALRPSLGILTDFQNPHLQQALNFRMSNFRNPIPS-NARPSSPFSRPMSQV-SG 720
           QSVQASTALRPS G+LTDFQNPHLQQALN RMS  RN  PS N RPS PFSR MSQV  G
Sbjct: 661 QSVQASTALRPSRGLLTDFQNPHLQQALNLRMSQLRNQNPSNNVRPSLPFSRAMSQVGGG 720

Query: 721 YGGSAYAAVT--SQHARMMAISQRAELMRQSSAMPLQNQTPRSAHSLQTTPDGLGI-SAG 780
           Y G +YAAVT  SQ+ARMM  SQRAELMRQSSAM LQNQT RSAHSLQTTPDGL + +AG
Sbjct: 721 YSGPSYAAVTPNSQNARMMVASQRAELMRQSSAMSLQNQTFRSAHSLQTTPDGLRMPTAG 780

Query: 781 DLRHVGGMSQSVTTAAGSVDLTSEQNWQPSGRMRGSLSGRAYSDAYGHLIIQPTQSSQSA 840
           +LR+VGGMSQSVT AAG V+ +SEQNWQPSGRMRGSLSGRA+SDA+GHLII PTQ  QSA
Sbjct: 781 ELRNVGGMSQSVTPAAGLVEPSSEQNWQPSGRMRGSLSGRAFSDAHGHLIIHPTQPVQSA 827

Query: 841 QPPSNSTPTQPGALFTQA 845
           +PPSNSTPTQP A  TQA
Sbjct: 841 RPPSNSTPTQPSAPSTQA 827

BLAST of Sed0011553 vs. NCBI nr
Match: XP_023533603.1 (E4 SUMO-protein ligase PIAL2-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1223.0 bits (3163), Expect = 0.0e+00
Identity = 651/859 (75.79%), Postives = 708/859 (82.42%), Query Frame = 0

Query: 1   MGAPSPYDLTMNRISLYIDGLTSHVNRIDQIDPAHFCSLCFSLARCIDFAIASNFVPSKV 60
           MG P PY++ +NRISLYIDG+TSHVNR DQIDP +FC+LCFSLARCIDFAIA+NFVPS V
Sbjct: 1   MGTPLPYEMYLNRISLYIDGITSHVNRTDQIDPVYFCNLCFSLARCIDFAIANNFVPSNV 60

Query: 61  HGLPTLLKQMCQKKHSHSHHLKAAIVVVLVSTKSACKARWFSEKEAEELYSLANEIGSDF 120
           HGLP+LLKQM QKK  HSH LKAA++V+++STK+ACK RWFSEKEAEELYSLANEIGSDF
Sbjct: 61  HGLPSLLKQMYQKK--HSHRLKAAVMVLMISTKNACKVRWFSEKEAEELYSLANEIGSDF 120

Query: 121 FGDSNIGQSNSLTAITTVMERFFPHLKLGQIVASMEVKPGYGVFTIHFNISKTMQYTPQE 180
           FGD+N G +NSLT IT VMERFFPHLKLGQIVA MEVKPGYGVF   FNISKTMQ + QE
Sbjct: 121 FGDTNTGPNNSLTTITAVMERFFPHLKLGQIVADMEVKPGYGVFATDFNISKTMQLSQQE 180

Query: 181 KIRLFVAQKDNTETSACIVSPPQVNFLVNGIGVNGRTISYMDTGPQLPTNVTHLLKLGSN 240
           KI LFVAQKDNTETSACI+SPPQVNFLVNG GVNGRT  +MDTGPQLPTNVTH+LKLG+N
Sbjct: 181 KILLFVAQKDNTETSACIISPPQVNFLVNGKGVNGRTNIFMDTGPQLPTNVTHMLKLGAN 240

Query: 241 LFQAIGSFNGHYVIAVAIMGTAPLPDSSVLQDHVQPVVSTVDSDSDIIEGPSRISLNCPI 300
           L QAIG+FNGHYVIAVAIMGTAPLPDSSVLQD+VQ VVSTVD DSDIIEGPSRISLNCPI
Sbjct: 241 LLQAIGNFNGHYVIAVAIMGTAPLPDSSVLQDYVQTVVSTVDPDSDIIEGPSRISLNCPI 300

Query: 301 SYTRIKVPVKGRSCKHLQCFDFYNFIDINSRRPSWRCPHCNQYICFLDIRVDQNMLKVIK 360
           SYTRIKVPVK RSCKHLQCFDFYNFI INSRRPSWRCPHCNQYICFLDIRVDQNMLKVI+
Sbjct: 301 SYTRIKVPVKSRSCKHLQCFDFYNFIGINSRRPSWRCPHCNQYICFLDIRVDQNMLKVIR 360

Query: 361 EVAENVTEVIISADGSWKAILENDDRNGQSLDDSLNHQNEMEQQESTTAPDVVDLTQVDD 420
           EVAENVTEVIISADGSWKAIL+N D  G+ LDDSLN QNE +Q+  +T P V+DL +VDD
Sbjct: 361 EVAENVTEVIISADGSWKAILDNGD--GRPLDDSLNLQNERDQE--STVPVVLDLIEVDD 420

Query: 421 DMDICDLEIEDTKPCLGNQNQLLSSGLNTPSEMNSNSSNPNFAAVLDDDFWSGIETGEIL 480
           D++ICDLEIED KPCLGN+                     NFAAVLDDDFWSGI+T  IL
Sbjct: 421 DINICDLEIEDEKPCLGNK---------------------NFAAVLDDDFWSGIDTDRIL 480

Query: 481 TSSTRSD--------APNFTGLMQLAGLTDPAIPAL-NHVVGVPGPVTFSSPALYDQNNL 540
           TSS R+D        APNF G MQ A LT+P  P L NH  GVPG V FSSPALYDQNNL
Sbjct: 481 TSSARTDAPIGNNPPAPNFAGHMQSAVLTNPVTPVLNNHGAGVPGHVIFSSPALYDQNNL 540

Query: 541 QIHVLTSNDNNQYGRMASMVRPSFTVSSRTPTAVQALPAQSQASGQPYGSRTPTISSAPQ 600
           Q   L SN+N++YGR  S+ RP     SR PT  QA P+ SQASGQ Y SRT TISSAPQ
Sbjct: 541 QSQALNSNENSEYGRTTSIARP----LSRMPTTAQAFPSPSQASGQQYSSRTTTISSAPQ 600

Query: 601 VGQNIPINRDGLNTISLDSERRQQLPRHPGDSPHATNPSPFHHPQTMQNRDPQDRSFTHG 660
           VG +IP NRD LNTIS DSER QQ PR PGDS HATN +PFHHP T+QNRDP D SFT G
Sbjct: 601 VGPSIPTNRDVLNTISRDSERSQQFPRRPGDSHHATNLAPFHHPPTLQNRDPLDHSFTPG 660

Query: 661 QSVQASTALRPSLGILTDFQNPHLQQALNFRMSNFRNPIPS-NARPSSPFSRPMSQV-SG 720
           QSVQASTALRPS G+LTDFQNPHLQQALN RMS  RN  PS N RPS PF R MSQV  G
Sbjct: 661 QSVQASTALRPSRGLLTDFQNPHLQQALNLRMSQLRNQNPSNNVRPSLPFLRAMSQVGGG 720

Query: 721 YGGSAYAAVT--SQHARMMAISQRAELMRQSSAMPLQNQTPRSAHSLQTTPDGLGI-SAG 780
           Y G +YAAVT  SQ+ARMM  SQRAELMRQSSAM LQNQT RSAHSLQTTPDGL + +AG
Sbjct: 721 YSGPSYAAVTPNSQNARMMVASQRAELMRQSSAMSLQNQTFRSAHSLQTTPDGLRMPAAG 780

Query: 781 DLRHVGGMSQSVTTAAGSVDLTSEQNWQPSGRMRGSLSGRAYSDAYGHLIIQPTQSSQSA 840
           +LR+VGGMSQSVT AAG VD +S+ NWQPSGRMRGSLSGRA+SDA+GHLII PTQS QSA
Sbjct: 781 ELRNVGGMSQSVTPAAGLVDPSSDLNWQPSGRMRGSLSGRAFSDAHGHLIIHPTQSVQSA 828

Query: 841 QPPSNSTPTQPGALFTQAQ 846
           +PPSNSTPTQP A  TQAQ
Sbjct: 841 RPPSNSTPTQPSAPSTQAQ 828

BLAST of Sed0011553 vs. NCBI nr
Match: XP_022995762.1 (E4 SUMO-protein ligase PIAL2-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 1222.6 bits (3162), Expect = 0.0e+00
Identity = 655/869 (75.37%), Postives = 713/869 (82.05%), Query Frame = 0

Query: 1   MGAPSPYDLTMNRISLYIDGLTSHVNRIDQIDPAHFCSLCFSLARCIDFAIASNFVPSKV 60
           MG P PY++  NRISLYIDG+TSHVNR DQIDPA+FC+LCFSLARCIDFAIA+NFVPS V
Sbjct: 1   MGTPLPYEMYSNRISLYIDGITSHVNRYDQIDPAYFCNLCFSLARCIDFAIANNFVPSNV 60

Query: 61  HGLPTLLKQMCQKKHSHSHHLKAAIVVVLVSTKSACKARWFSEKEAEELYSLANEIGSDF 120
           HGLP LLKQM QKK  HSH LKAA++V+++STK+ACK RWFSEKEAE+LYSLANEIGSDF
Sbjct: 61  HGLPNLLKQMYQKK--HSHRLKAAVMVLMISTKNACKVRWFSEKEAEDLYSLANEIGSDF 120

Query: 121 FGDSNIGQSNSLTAITTVMERFFPHLKLGQIVASMEVKPGYGVFTIHFNISKTMQYTPQE 180
           FGD+N G +NSLT IT VMERFFPHLKLGQIVA+MEVKPGYGVF   FNISKTMQ++ Q+
Sbjct: 121 FGDTNTGPNNSLTTITAVMERFFPHLKLGQIVAAMEVKPGYGVFATDFNISKTMQFSQQD 180

Query: 181 KIRLFVAQKDNTETSACIVSPPQVNFLVNGIGVNGRTISYMDTGPQLPTNVTHLLKLGSN 240
           KI LFVAQKDNTETSACI+SPPQVNFLVNG GVNGRT  +MDTGPQLPTNVTH+LKLG+N
Sbjct: 181 KILLFVAQKDNTETSACIISPPQVNFLVNGKGVNGRTNIFMDTGPQLPTNVTHMLKLGAN 240

Query: 241 LFQAIGSFNGHYVIAVAIMGTAPLPDSSVLQDHVQPVVSTVDSDSDIIEGPSRISLNCPI 300
           L QAIG+FNGHYVIAVAIMGTAPLPDSSVLQD+VQPVVSTVDSDSDIIEGPSRISLNCPI
Sbjct: 241 LLQAIGNFNGHYVIAVAIMGTAPLPDSSVLQDYVQPVVSTVDSDSDIIEGPSRISLNCPI 300

Query: 301 SYTRIKVPVKGRSCKHLQ----------CFDFYNFIDINSRRPSWRCPHCNQYICFLDIR 360
           SYTRIKVPVK RSCKHLQ          CFDFYNFI INSRRPSWRCPHCNQYICFLDIR
Sbjct: 301 SYTRIKVPVKSRSCKHLQVSISCHGIWKCFDFYNFIGINSRRPSWRCPHCNQYICFLDIR 360

Query: 361 VDQNMLKVIKEVAENVTEVIISADGSWKAILENDDRNGQSLDDSLNHQNEMEQQESTTAP 420
           VDQNM+KVI+EVAENVTEVIISADGSWKAILEND+ +G+ LDDSLN QNE +Q+  +T P
Sbjct: 361 VDQNMMKVIREVAENVTEVIISADGSWKAILENDNGDGRPLDDSLNLQNERDQE--STVP 420

Query: 421 DVVDLTQVDDDMDICDLEIEDTKPCLGNQNQLLSSGLNTPSEMNSNSSNPNFAAVLDDDF 480
           DV+DL +VDDD++ICDLEIED KPCLGN+                     NFAAVLDDDF
Sbjct: 421 DVLDLIEVDDDINICDLEIEDEKPCLGNK---------------------NFAAVLDDDF 480

Query: 481 WSGIETGEILTSSTRSD--------APNFTGLMQLAGLTDPAIPAL-NHVVGVPGPVTFS 540
           WSGI+T  ILTSS R+D        APNF GLMQ A LT+P  P L NH  GVPG V F 
Sbjct: 481 WSGIDTDRILTSSARTDAPIGNNPPAPNFAGLMQSAVLTNPVTPVLNNHGAGVPGHVIFL 540

Query: 541 SPALYDQNNLQIHVLTSNDNNQYGRMASMVRPSFTVSSRTPTAVQALPAQSQASGQPYGS 600
           SPALYDQNNLQ   L SN+N +YGR  S+ RP     SR PT  QALP  SQASGQ Y S
Sbjct: 541 SPALYDQNNLQTQALNSNENTEYGRTTSIARP----LSRMPTTAQALPYPSQASGQQYSS 600

Query: 601 RTPTISSAPQVGQNIPINRDGLNTISLDSERRQQLPRHPGDSPHATNPSPFHHPQTMQNR 660
           RT TISSA QVG +IP NRDGLNTIS DSER QQ PRHPGDS HATN +PFHHP T QNR
Sbjct: 601 RTTTISSASQVGPSIPTNRDGLNTISRDSERSQQFPRHPGDSHHATNLAPFHHPPTSQNR 660

Query: 661 DPQDRSFTHGQSVQASTALRPSLGILTDFQNPHLQQALNFRMSNFRNPIPS-NARPSSPF 720
           DP   SFT GQSVQASTALRPS  +LTDFQNPHLQQALN RMS  RN  PS N RPS PF
Sbjct: 661 DP--HSFTPGQSVQASTALRPSTRLLTDFQNPHLQQALNLRMSQLRNQNPSNNVRPSLPF 720

Query: 721 SRPMSQV-SGYGGSAYAAVT--SQHARMMAISQRAELMRQSSAMPLQNQTPRSAHSLQTT 780
           SR MSQV  GY G +YAAVT  SQ+ARM+A SQRAELMRQSSAM LQNQT RSAHSLQTT
Sbjct: 721 SRAMSQVGGGYSGPSYAAVTPNSQNARMVA-SQRAELMRQSSAMSLQNQTFRSAHSLQTT 780

Query: 781 PDGLGI-SAGDLRHVGGMSQSVTTAAGSVDLTSEQNWQPSGRMRGSLSGRAYSDAYGHLI 840
           PDGL + +AG+LR+VGGMSQSVT AAG VD +SEQNWQPSGRMRGSLSGRA+SDA+GHLI
Sbjct: 781 PDGLRMPAAGELRNVGGMSQSVTLAAGLVDPSSEQNWQPSGRMRGSLSGRAFSDAHGHLI 837

Query: 841 IQPTQSSQSAQPPSNSTPTQPGALFTQAQ 846
           I PTQS QSA+PPSN TPTQP A  TQAQ
Sbjct: 841 IHPTQSVQSARPPSNPTPTQPSAPSTQAQ 837

BLAST of Sed0011553 vs. ExPASy Swiss-Prot
Match: P22503 (Endoglucanase OS=Phaseolus vulgaris OX=3885 PE=2 SV=2)

HSP 1 Score: 603.2 bits (1554), Expect = 6.5e-171
Identity = 288/395 (72.91%), Postives = 335/395 (84.81%), Query Frame = 0

Query: 837  PGALFTQAQSWTVVEYEKEISSVNQLEHLRNSIRWGADFILRAHVSATTLYTQVGDGNGD 896
            P A  T   SW  VEYE EISSVNQL +L+++IRWGADF+LRAH S TTLYTQVGDGN D
Sbjct: 100  PMAFSTSLLSWAAVEYESEISSVNQLGYLQSAIRWGADFMLRAHTSPTTLYTQVGDGNAD 159

Query: 897  HQCWERPEDMDTPRTLYKITSASPGTEAAAEAAAALAATSIVFNHVDTNYSRTLLQHSIS 956
            H CWERPEDMDTPRT+YKI + SPGTE AAE AAAL+A SIVF  +D  YS TLL HS S
Sbjct: 160  HNCWERPEDMDTPRTVYKIDANSPGTEVAAEYAAALSAASIVFKKIDAKYSSTLLSHSKS 219

Query: 957  LFQFADQFRGSYSASCPFYCSFSGYQDELLWAAAWLYKATGTNKYLSYVISNQGWSQAVS 1016
            LF FAD+ RGSYS SCPFYCS+SGYQDELLWAAAWLYKA+G +KYLSY+ISNQGWSQ VS
Sbjct: 220  LFDFADKNRGSYSGSCPFYCSYSGYQDELLWAAAWLYKASGESKYLSYIISNQGWSQTVS 279

Query: 1017 EFSWDNKFVGAQTLLAKEFYKGMKELGKFKSDVESFICALMPGSGSTQIRTTPGGLLFVR 1076
            EFSWDNKFVGAQTLL +EFY G K+L K K+D ESFICA+MPGS S QI+TTPGGLLF R
Sbjct: 280  EFSWDNKFVGAQTLLTEEFYGGKKDLAKIKTDAESFICAVMPGSNSRQIKTTPGGLLFTR 339

Query: 1077 DSSNLQYVSSSSMVLFMYSKLLNQARVDGVHCRSKIFSSSQIKSFAKSQVDYILGNNPLK 1136
            DSSNLQY +SS+MVLF++S++LN+  ++G++C S  F++SQI+ FAK+QV+YILG NP+K
Sbjct: 340  DSSNLQYTTSSTMVLFIFSRILNRNHINGINCGSSHFTASQIRGFAKTQVEYILGKNPMK 399

Query: 1137 MSYMVGFGSKYPLQLHHRASSIPSIRTYVTKVGCSDGYSKYYNSNNPNPNIHVGAIVGGP 1196
            MSYMVGFGSKYP QLHHR SSIPSI+ +  KVGC+ G S YYNS NPNPN HVGAIVGGP
Sbjct: 400  MSYMVGFGSKYPKQLHHRGSSIPSIKVHPAKVGCNAGLSDYYNSANPNPNTHVGAIVGGP 459

Query: 1197 DSNDRFSDTRSDHTHSEPTTYMNAAFVGSVAALVA 1232
            DSNDRF+D RSD++H+EPTTY+NAAFV S++AL+A
Sbjct: 460  DSNDRFNDARSDYSHAEPTTYINAAFVASISALLA 494

BLAST of Sed0011553 vs. ExPASy Swiss-Prot
Match: Q9SUS0 (Endoglucanase 20 OS=Arabidopsis thaliana OX=3702 GN=At4g23560 PE=2 SV=1)

HSP 1 Score: 572.0 bits (1473), Expect = 1.6e-161
Identity = 271/395 (68.61%), Postives = 329/395 (83.29%), Query Frame = 0

Query: 837  PGALFTQAQSWTVVEYEKEISSVNQLEHLRNSIRWGADFILRAHVSATTLYTQVGDGNGD 896
            P +  T   SW  +EY+ EISSVNQL +LR++I+WG DFILRAH S   LYTQVGDGN D
Sbjct: 83   PMSFTTTLLSWAAIEYQNEISSVNQLGYLRSTIKWGTDFILRAHTSPNMLYTQVGDGNSD 142

Query: 897  HQCWERPEDMDTPRTLYKITSASPGTEAAAEAAAALAATSIVFNHVDTNYSRTLLQHSIS 956
            H CWERPEDMDT RTLY I+S+SPG+EAA EAAAALAA S+VF  VD+ YS TLL H+ +
Sbjct: 143  HSCWERPEDMDTSRTLYSISSSSPGSEAAGEAAAALAAASLVFKSVDSTYSSTLLNHAKT 202

Query: 957  LFQFADQFRGSYSASCPFYCSFSGYQDELLWAAAWLYKATGTNKYLSYVISNQGWSQAVS 1016
            LF+FAD++RGSY ASCPFYCS+SGYQDELLWAAAWLYKATG   Y++YVISN+ WSQAV+
Sbjct: 203  LFEFADKYRGSYQASCPFYCSYSGYQDELLWAAAWLYKATGDKIYINYVISNKDWSQAVN 262

Query: 1017 EFSWDNKFVGAQTLLAKEFYKGMKELGKFKSDVESFICALMPGSGSTQIRTTPGGLLFVR 1076
            EFSWDNKFVGAQ LL  EFY G  +L KFKSDVESF+CA+MPGS S QI+ TPGGLLF+R
Sbjct: 263  EFSWDNKFVGAQALLVSEFYNGANDLAKFKSDVESFVCAMMPGSSSQQIKPTPGGLLFIR 322

Query: 1077 DSSNLQYVSSSSMVLFMYSKLLNQARVDGVHCRSKIFSSSQIKSFAKSQVDYILGNNPLK 1136
            DSSNLQYV++++ VLF YSK L +A V  + C S  F+ SQI++FAKSQVDYILGNNP+K
Sbjct: 323  DSSNLQYVTTATTVLFHYSKTLTKAGVGSIQCGSTKFTVSQIRNFAKSQVDYILGNNPMK 382

Query: 1137 MSYMVGFGSKYPLQLHHRASSIPSIRTYVTKVGCSDGYSKYYNSNNPNPNIHVGAIVGGP 1196
            MSYMVGFG+KYP Q HHR SS+PSI++   K+ C+ GYS YYNS+ PNPN+H+GAIVGGP
Sbjct: 383  MSYMVGFGTKYPTQPHHRGSSLPSIQSKPEKIDCNGGYS-YYNSDTPNPNVHIGAIVGGP 442

Query: 1197 DSNDRFSDTRSDHTHSEPTTYMNAAFVGSVAALVA 1232
            +S+D++SD +SD++H+EPTTY+NAAF+G VAAL++
Sbjct: 443  NSSDQYSDKKSDYSHAEPTTYINAAFIGPVAALIS 476

BLAST of Sed0011553 vs. ExPASy Swiss-Prot
Match: Q9SZ90 (Endoglucanase 18 OS=Arabidopsis thaliana OX=3702 GN=At4g09740 PE=3 SV=2)

HSP 1 Score: 557.4 bits (1435), Expect = 4.1e-157
Identity = 264/395 (66.84%), Postives = 325/395 (82.28%), Query Frame = 0

Query: 837  PGALFTQAQSWTVVEYEKEISSVNQLEHLRNSIRWGADFILRAHVSATTLYTQVGDGNGD 896
            P +  T   SW  +EY+ EI+ VNQL +LR++I+WG +FILRAH S   LYTQVGDGN D
Sbjct: 83   PMSFTTTLLSWAALEYQNEITFVNQLGYLRSTIKWGTNFILRAHTSTNMLYTQVGDGNSD 142

Query: 897  HQCWERPEDMDTPRTLYKITSASPGTEAAAEAAAALAATSIVFNHVDTNYSRTLLQHSIS 956
            H CWERPEDMDTPRTLY I+S+SPG+EAA EAAAALAA S+VF  VD+ YS  LL ++ S
Sbjct: 143  HSCWERPEDMDTPRTLYSISSSSPGSEAAGEAAAALAAASLVFKLVDSTYSSKLLNNAKS 202

Query: 957  LFQFADQFRGSYSASCPFYCSFSGYQDELLWAAAWLYKATGTNKYLSYVISNQGWSQAVS 1016
            LF+FAD++RGSY ASCPFYCS SGYQDELLWAAAWLYKATG   YL+YVISN+ WS+A++
Sbjct: 203  LFEFADKYRGSYQASCPFYCSHSGYQDELLWAAAWLYKATGEKSYLNYVISNKDWSKAIN 262

Query: 1017 EFSWDNKFVGAQTLLAKEFYKGMKELGKFKSDVESFICALMPGSGSTQIRTTPGGLLFVR 1076
            EFSWDNKF G Q LLA EFY G  +L KFK+DVESF+CALMPGS S QI+ TPGG+LF+R
Sbjct: 263  EFSWDNKFAGVQALLASEFYNGANDLEKFKTDVESFVCALMPGSSSQQIKPTPGGILFIR 322

Query: 1077 DSSNLQYVSSSSMVLFMYSKLLNQARVDGVHCRSKIFSSSQIKSFAKSQVDYILGNNPLK 1136
            DSSNLQYV++++ +LF YSK L +A V  + C S  F+ SQI++FAKSQVDYILGNNPLK
Sbjct: 323  DSSNLQYVTTATTILFYYSKTLTKAGVGSIQCGSTQFTVSQIRNFAKSQVDYILGNNPLK 382

Query: 1137 MSYMVGFGSKYPLQLHHRASSIPSIRTYVTKVGCSDGYSKYYNSNNPNPNIHVGAIVGGP 1196
            MSYMVGFG+KYP Q HHR SS+PSI++   K+ C+ G+S YYN + PNPN+H GAIVGGP
Sbjct: 383  MSYMVGFGTKYPTQPHHRGSSLPSIQSKPEKIDCNGGFS-YYNFDTPNPNVHTGAIVGGP 442

Query: 1197 DSNDRFSDTRSDHTHSEPTTYMNAAFVGSVAALVA 1232
            +S+D++SD R+D++H+EPTTY+NAAF+GSVAAL++
Sbjct: 443  NSSDQYSDKRTDYSHAEPTTYINAAFIGSVAALIS 476

BLAST of Sed0011553 vs. ExPASy Swiss-Prot
Match: Q6ZA06 (Endoglucanase 20 OS=Oryza sativa subsp. japonica OX=39947 GN=GLU15 PE=2 SV=1)

HSP 1 Score: 496.9 bits (1278), Expect = 6.6e-139
Identity = 240/393 (61.07%), Postives = 302/393 (76.84%), Query Frame = 0

Query: 837  PGALFTQAQSWTVVEYEKEISSVNQLEHLRNSIRWGADFILRAHVSATTLYTQVGDGNGD 896
            P A       W+ VEY   +++  +L +LR +IRWGADF+LRAH S TTLYTQVGDGN D
Sbjct: 100  PMAFTVTLLGWSAVEYGAAVAAAGELGNLRAAIRWGADFLLRAHASPTTLYTQVGDGNAD 159

Query: 897  HQCWERPEDMDTPRTLYKITSASPGTEAAAEAAAALAATSIVF-NHVDTNYSRTLLQHSI 956
            HQCWERPEDMDTPRTLYKIT+ SPG+EAAAEA+AALAA  +   +  DT +S  LL  S 
Sbjct: 160  HQCWERPEDMDTPRTLYKITADSPGSEAAAEASAALAAAYVALKDDGDTAFSSRLLAASR 219

Query: 957  SLFQFADQFRGSYSASCPFYCSFSGYQDELLWAAAWLYKATGTNKYLSYVISNQGWSQAV 1016
            SLF FA+ +RGS+ +SCPFYCS+SG+QDELLWA+AWL+KAT   KYL ++ +NQG S  V
Sbjct: 220  SLFDFANNYRGSFQSSCPFYCSYSGFQDELLWASAWLFKATRDAKYLDFLTNNQGSSNPV 279

Query: 1017 SEFSWDNKFVGAQTLLAKEFYKGMKELGKFKSDVESFICALMPGSGSTQIRTTPGGLLFV 1076
            +EFSWDNK+ GAQ L A+E+  G  +L ++K +++SF+CALMP SG+ QIRTTPGGLLF 
Sbjct: 280  NEFSWDNKYAGAQMLAAQEYLGGRTQLARYKDNLDSFVCALMPNSGNVQIRTTPGGLLFT 339

Query: 1077 RDSSNLQYVSSSSMVLFMYSKLLNQARVDGVHCRSKIFSSSQIKSFAKSQVDYILGNNPL 1136
            RDS NLQY +++++VL +YSK+L  +   GV C +  FS +QI SFA SQVDYILG NPL
Sbjct: 340  RDSVNLQYTTTATLVLSIYSKVLKSSGSRGVRCSAATFSPNQISSFATSQVDYILGKNPL 399

Query: 1137 KMSYMVGFGSKYPLQLHHRASSIPSIRTYVTKVGCSDGYSKYYNSNNPNPNIHVGAIVGG 1196
             MSYMVGF +K+P ++HHR SSIPSI+    KV C +G+S +  +++PNPNIHVGAIVGG
Sbjct: 400  GMSYMVGFSTKFPRRIHHRGSSIPSIKVLSRKVTCKEGFSSWLPTSDPNPNIHVGAIVGG 459

Query: 1197 PDSNDRFSDTRSDHTHSEPTTYMNAAFVGSVAA 1229
            PD ND+FSD R D +HSEP TY+NAAFVG+ AA
Sbjct: 460  PDGNDQFSDNRGDSSHSEPATYINAAFVGACAA 492

BLAST of Sed0011553 vs. ExPASy Swiss-Prot
Match: Q9SRX3 (Endoglucanase 1 OS=Arabidopsis thaliana OX=3702 GN=CEL2 PE=2 SV=1)

HSP 1 Score: 403.3 bits (1035), Expect = 9.9e-111
Identity = 206/402 (51.24%), Postives = 280/402 (69.65%), Query Frame = 0

Query: 837  PGALFTQAQSWTVVEYEKEISSVNQLEHLRNSIRWGADFILRAHVSATTLYTQVGDGNGD 896
            P A  T   SW+++E+   + S  +L + +++IRW  DF+L+A     T+Y QVGD N D
Sbjct: 103  PMAFTTTMLSWSLIEFGGLMKS--ELPNAKDAIRWATDFLLKATSHPDTIYVQVGDPNMD 162

Query: 897  HQCWERPEDMDTPRTLYKITSASPGTEAAAEAAAALAATSIVFNHVDTNYSRTLLQHSIS 956
            H CWERPEDMDTPR+++K+   +PG++ A E AAALAA SIVF   D +YS  LLQ +I+
Sbjct: 163  HACWERPEDMDTPRSVFKVDKNNPGSDIAGEIAAALAAASIVFRKCDPSYSNHLLQRAIT 222

Query: 957  LFQFADQFRGSYSAS-----CPFYCSFSGYQDELLWAAAWLYKATGTNKYLSYVISN--- 1016
            +F FAD++RG YSA      CPFYCS+SGYQDELLW AAWL KAT    YL+Y+ +N   
Sbjct: 223  VFTFADKYRGPYSAGLAPEVCPFYCSYSGYQDELLWGAAWLQKATNNPTYLNYIKANGQI 282

Query: 1017 QGWSQAVSEFSWDNKFVGAQTLLAKEF-YKGMKELGKFKSDVESFICALMPGSGSTQIRT 1076
             G  +  + FSWDNK VGA+ LL+KEF  + +K L ++K   +SFIC+++PG+ S+Q   
Sbjct: 283  LGADEFDNMFSWDNKHVGARILLSKEFLIQKVKSLEEYKEHADSFICSVLPGASSSQY-- 342

Query: 1077 TPGGLLFVRDSSNLQYVSSSSMVLFMYSKLLNQARVDGVHCRSKIFSSSQIKSFAKSQVD 1136
            TPGGLLF    SN+QYV+S+S +L  Y+K L  AR    +C   + + ++++S AK QVD
Sbjct: 343  TPGGLLFKMGESNMQYVTSTSFLLLTYAKYLTSART-VAYCGGSVVTPARLRSIAKKQVD 402

Query: 1137 YILGNNPLKMSYMVGFGSKYPLQLHHRASSIPSIRTYVTKVGCSDGYSKYYNSNNPNPNI 1196
            Y+LG NPLKMSYMVG+G KYP ++HHR SS+PS+  + T++ C DG+S  + S +PNPN 
Sbjct: 403  YLLGGNPLKMSYMVGYGLKYPRRIHHRGSSLPSVAVHPTRIQCHDGFS-LFTSQSPNPND 462

Query: 1197 HVGAIVGGPDSNDRFSDTRSDHTHSEPTTYMNAAFVGSVAAL 1230
             VGA+VGGPD ND+F D RSD+  SEP TY+NA  VG++A L
Sbjct: 463  LVGAVVGGPDQNDQFPDERSDYGRSEPATYINAPLVGALAYL 498

BLAST of Sed0011553 vs. ExPASy TrEMBL
Match: A0A6J1K8Y8 (E4 SUMO-protein ligase PIAL2-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111491198 PE=4 SV=1)

HSP 1 Score: 1230.7 bits (3183), Expect = 0.0e+00
Identity = 655/859 (76.25%), Postives = 713/859 (83.00%), Query Frame = 0

Query: 1   MGAPSPYDLTMNRISLYIDGLTSHVNRIDQIDPAHFCSLCFSLARCIDFAIASNFVPSKV 60
           MG P PY++  NRISLYIDG+TSHVNR DQIDPA+FC+LCFSLARCIDFAIA+NFVPS V
Sbjct: 1   MGTPLPYEMYSNRISLYIDGITSHVNRYDQIDPAYFCNLCFSLARCIDFAIANNFVPSNV 60

Query: 61  HGLPTLLKQMCQKKHSHSHHLKAAIVVVLVSTKSACKARWFSEKEAEELYSLANEIGSDF 120
           HGLP LLKQM QKK  HSH LKAA++V+++STK+ACK RWFSEKEAE+LYSLANEIGSDF
Sbjct: 61  HGLPNLLKQMYQKK--HSHRLKAAVMVLMISTKNACKVRWFSEKEAEDLYSLANEIGSDF 120

Query: 121 FGDSNIGQSNSLTAITTVMERFFPHLKLGQIVASMEVKPGYGVFTIHFNISKTMQYTPQE 180
           FGD+N G +NSLT IT VMERFFPHLKLGQIVA+MEVKPGYGVF   FNISKTMQ++ Q+
Sbjct: 121 FGDTNTGPNNSLTTITAVMERFFPHLKLGQIVAAMEVKPGYGVFATDFNISKTMQFSQQD 180

Query: 181 KIRLFVAQKDNTETSACIVSPPQVNFLVNGIGVNGRTISYMDTGPQLPTNVTHLLKLGSN 240
           KI LFVAQKDNTETSACI+SPPQVNFLVNG GVNGRT  +MDTGPQLPTNVTH+LKLG+N
Sbjct: 181 KILLFVAQKDNTETSACIISPPQVNFLVNGKGVNGRTNIFMDTGPQLPTNVTHMLKLGAN 240

Query: 241 LFQAIGSFNGHYVIAVAIMGTAPLPDSSVLQDHVQPVVSTVDSDSDIIEGPSRISLNCPI 300
           L QAIG+FNGHYVIAVAIMGTAPLPDSSVLQD+VQPVVSTVDSDSDIIEGPSRISLNCPI
Sbjct: 241 LLQAIGNFNGHYVIAVAIMGTAPLPDSSVLQDYVQPVVSTVDSDSDIIEGPSRISLNCPI 300

Query: 301 SYTRIKVPVKGRSCKHLQCFDFYNFIDINSRRPSWRCPHCNQYICFLDIRVDQNMLKVIK 360
           SYTRIKVPVK RSCKHLQCFDFYNFI INSRRPSWRCPHCNQYICFLDIRVDQNM+KVI+
Sbjct: 301 SYTRIKVPVKSRSCKHLQCFDFYNFIGINSRRPSWRCPHCNQYICFLDIRVDQNMMKVIR 360

Query: 361 EVAENVTEVIISADGSWKAILENDDRNGQSLDDSLNHQNEMEQQESTTAPDVVDLTQVDD 420
           EVAENVTEVIISADGSWKAILEND+ +G+ LDDSLN QNE +Q+  +T PDV+DL +VDD
Sbjct: 361 EVAENVTEVIISADGSWKAILENDNGDGRPLDDSLNLQNERDQE--STVPDVLDLIEVDD 420

Query: 421 DMDICDLEIEDTKPCLGNQNQLLSSGLNTPSEMNSNSSNPNFAAVLDDDFWSGIETGEIL 480
           D++ICDLEIED KPCLGN+                     NFAAVLDDDFWSGI+T  IL
Sbjct: 421 DINICDLEIEDEKPCLGNK---------------------NFAAVLDDDFWSGIDTDRIL 480

Query: 481 TSSTRSD--------APNFTGLMQLAGLTDPAIPAL-NHVVGVPGPVTFSSPALYDQNNL 540
           TSS R+D        APNF GLMQ A LT+P  P L NH  GVPG V F SPALYDQNNL
Sbjct: 481 TSSARTDAPIGNNPPAPNFAGLMQSAVLTNPVTPVLNNHGAGVPGHVIFLSPALYDQNNL 540

Query: 541 QIHVLTSNDNNQYGRMASMVRPSFTVSSRTPTAVQALPAQSQASGQPYGSRTPTISSAPQ 600
           Q   L SN+N +YGR  S+ RP     SR PT  QALP  SQASGQ Y SRT TISSA Q
Sbjct: 541 QTQALNSNENTEYGRTTSIARP----LSRMPTTAQALPYPSQASGQQYSSRTTTISSASQ 600

Query: 601 VGQNIPINRDGLNTISLDSERRQQLPRHPGDSPHATNPSPFHHPQTMQNRDPQDRSFTHG 660
           VG +IP NRDGLNTIS DSER QQ PRHPGDS HATN +PFHHP T QNRDP   SFT G
Sbjct: 601 VGPSIPTNRDGLNTISRDSERSQQFPRHPGDSHHATNLAPFHHPPTSQNRDP--HSFTPG 660

Query: 661 QSVQASTALRPSLGILTDFQNPHLQQALNFRMSNFRNPIPS-NARPSSPFSRPMSQV-SG 720
           QSVQASTALRPS  +LTDFQNPHLQQALN RMS  RN  PS N RPS PFSR MSQV  G
Sbjct: 661 QSVQASTALRPSTRLLTDFQNPHLQQALNLRMSQLRNQNPSNNVRPSLPFSRAMSQVGGG 720

Query: 721 YGGSAYAAVT--SQHARMMAISQRAELMRQSSAMPLQNQTPRSAHSLQTTPDGLGI-SAG 780
           Y G +YAAVT  SQ+ARM+A SQRAELMRQSSAM LQNQT RSAHSLQTTPDGL + +AG
Sbjct: 721 YSGPSYAAVTPNSQNARMVA-SQRAELMRQSSAMSLQNQTFRSAHSLQTTPDGLRMPAAG 780

Query: 781 DLRHVGGMSQSVTTAAGSVDLTSEQNWQPSGRMRGSLSGRAYSDAYGHLIIQPTQSSQSA 840
           +LR+VGGMSQSVT AAG VD +SEQNWQPSGRMRGSLSGRA+SDA+GHLII PTQS QSA
Sbjct: 781 ELRNVGGMSQSVTLAAGLVDPSSEQNWQPSGRMRGSLSGRAFSDAHGHLIIHPTQSVQSA 827

Query: 841 QPPSNSTPTQPGALFTQAQ 846
           +PPSN TPTQP A  TQAQ
Sbjct: 841 RPPSNPTPTQPSAPSTQAQ 827

BLAST of Sed0011553 vs. ExPASy TrEMBL
Match: A0A6J1H3W3 (E4 SUMO-protein ligase PIAL2-like isoform X4 OS=Cucurbita moschata OX=3662 GN=LOC111459766 PE=4 SV=1)

HSP 1 Score: 1223.8 bits (3165), Expect = 0.0e+00
Identity = 652/858 (75.99%), Postives = 710/858 (82.75%), Query Frame = 0

Query: 1   MGAPSPYDLTMNRISLYIDGLTSHVNRIDQIDPAHFCSLCFSLARCIDFAIASNFVPSKV 60
           MG P PY++ +NRISLYIDG+TS VNR DQIDPA+FC+LCFSLARCIDFAIA+NFVPS V
Sbjct: 1   MGTPLPYEMYLNRISLYIDGITSLVNRNDQIDPAYFCNLCFSLARCIDFAIANNFVPSNV 60

Query: 61  HGLPTLLKQMCQKKHSHSHHLKAAIVVVLVSTKSACKARWFSEKEAEELYSLANEIGSDF 120
           HGLP+LLKQM QKK  HSH LKAA++V+++STK+ACK RWFSEKEAEELYSLANEIGSDF
Sbjct: 61  HGLPSLLKQMYQKK--HSHRLKAAVMVLMISTKNACKVRWFSEKEAEELYSLANEIGSDF 120

Query: 121 FGDSNIGQSNSLTAITTVMERFFPHLKLGQIVASMEVKPGYGVFTIHFNISKTMQYTPQE 180
           FGD+N G +NSLT IT VMERFFPHLKLGQIVA MEVKPGYGVF   FNISKTMQ + QE
Sbjct: 121 FGDTNTGPNNSLTTITAVMERFFPHLKLGQIVADMEVKPGYGVFATDFNISKTMQLSQQE 180

Query: 181 KIRLFVAQKDNTETSACIVSPPQVNFLVNGIGVNGRTISYMDTGPQLPTNVTHLLKLGSN 240
           KI LFVAQKDNTETSACI+SPPQVNFLVNG GVNGRT  +MDTGPQLPTNVTH+LKLG+N
Sbjct: 181 KILLFVAQKDNTETSACIISPPQVNFLVNGKGVNGRTNIFMDTGPQLPTNVTHMLKLGAN 240

Query: 241 LFQAIGSFNGHYVIAVAIMGTAPLPDSSVLQDHVQPVVSTVDSDSDIIEGPSRISLNCPI 300
           L QAIG+FNGHYVIAVAIMGTAPLPDSSVLQD+VQ VVSTVD DSDIIEGPSRISLNCPI
Sbjct: 241 LLQAIGNFNGHYVIAVAIMGTAPLPDSSVLQDYVQTVVSTVDPDSDIIEGPSRISLNCPI 300

Query: 301 SYTRIKVPVKGRSCKHLQCFDFYNFIDINSRRPSWRCPHCNQYICFLDIRVDQNMLKVIK 360
           SYTRIKVPVK RSCKHLQCFDFYNFI INSRRPSWRCPHCNQYICFLDIRVDQNMLKVI+
Sbjct: 301 SYTRIKVPVKSRSCKHLQCFDFYNFIGINSRRPSWRCPHCNQYICFLDIRVDQNMLKVIR 360

Query: 361 EVAENVTEVIISADGSWKAILENDDRNGQSLDDSLNHQNEMEQQESTTAPDVVDLTQVDD 420
           EVAENVTEVIISADGSWKAIL+N D  G+ LDDSLN QNE +Q+  +T P V+DL +VDD
Sbjct: 361 EVAENVTEVIISADGSWKAILDNGD--GRPLDDSLNLQNERDQE--STVPVVLDLIEVDD 420

Query: 421 DMDICDLEIEDTKPCLGNQNQLLSSGLNTPSEMNSNSSNPNFAAVLDDDFWSGIETGEIL 480
           D++ICDLEIED KPC+GN+                     NFAAVLDDDFWSGI+T  IL
Sbjct: 421 DINICDLEIEDEKPCIGNK---------------------NFAAVLDDDFWSGIDTDRIL 480

Query: 481 TSSTRSD--------APNFTGLMQLAGLTDPAIPAL-NHVVGVPGPVTFSSPALYDQNNL 540
           TSS R+D        APNF GLMQ A LT+P  P L NH  GVPG V FSSPALYDQNNL
Sbjct: 481 TSSARTDAPIGNNPPAPNFAGLMQSAVLTNPVTPVLNNHGAGVPGHVIFSSPALYDQNNL 540

Query: 541 QIHVLTSNDNNQYGRMASMVRPSFTVSSRTPTAVQALPAQSQASGQPYGSRTPTISSAPQ 600
           Q   L SN+N++YGR  S+ RP     SR PT  QALP+ SQASGQ Y SRT TISSAPQ
Sbjct: 541 QSQALNSNENSEYGRTTSIARP----LSRMPTTAQALPSLSQASGQQYSSRTTTISSAPQ 600

Query: 601 VGQNIPINRDGLNTISLDSERRQQLPRHPGDSPHATNPSPFHHPQTMQNRDPQDRSFTHG 660
           VG +IP NRDGLNTIS DSER QQ PR PGDS HATN +PFHHP T+QN DP D SFT G
Sbjct: 601 VGPSIPTNRDGLNTISRDSERSQQFPRRPGDSHHATNLAPFHHPPTLQNWDPLDHSFTPG 660

Query: 661 QSVQASTALRPSLGILTDFQNPHLQQALNFRMSNFRNPIPS-NARPSSPFSRPMSQV-SG 720
           QSVQASTALRPS G+LTDFQNPHLQQALN RMS  RN  PS N RPS PFSR MSQV  G
Sbjct: 661 QSVQASTALRPSRGLLTDFQNPHLQQALNLRMSQLRNQNPSNNVRPSLPFSRAMSQVGGG 720

Query: 721 YGGSAYAAVT--SQHARMMAISQRAELMRQSSAMPLQNQTPRSAHSLQTTPDGLGI-SAG 780
           Y G +YAAVT  SQ+ARMM  SQRAELMRQSSAM LQNQT RSAHSLQTTPDGL + +AG
Sbjct: 721 YSGPSYAAVTPNSQNARMMVASQRAELMRQSSAMSLQNQTFRSAHSLQTTPDGLRMPTAG 780

Query: 781 DLRHVGGMSQSVTTAAGSVDLTSEQNWQPSGRMRGSLSGRAYSDAYGHLIIQPTQSSQSA 840
           +LR+VGGMSQSVT AAG V+ +SEQNWQPSGRMRGSLSGRA+SDA+GHLII PTQ  QSA
Sbjct: 781 ELRNVGGMSQSVTPAAGLVEPSSEQNWQPSGRMRGSLSGRAFSDAHGHLIIHPTQPVQSA 827

Query: 841 QPPSNSTPTQPGALFTQA 845
           +PPSNSTPTQP A  TQA
Sbjct: 841 RPPSNSTPTQPSAPSTQA 827

BLAST of Sed0011553 vs. ExPASy TrEMBL
Match: A0A6J1JZV7 (E4 SUMO-protein ligase PIAL2-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111491198 PE=4 SV=1)

HSP 1 Score: 1222.6 bits (3162), Expect = 0.0e+00
Identity = 655/869 (75.37%), Postives = 713/869 (82.05%), Query Frame = 0

Query: 1   MGAPSPYDLTMNRISLYIDGLTSHVNRIDQIDPAHFCSLCFSLARCIDFAIASNFVPSKV 60
           MG P PY++  NRISLYIDG+TSHVNR DQIDPA+FC+LCFSLARCIDFAIA+NFVPS V
Sbjct: 1   MGTPLPYEMYSNRISLYIDGITSHVNRYDQIDPAYFCNLCFSLARCIDFAIANNFVPSNV 60

Query: 61  HGLPTLLKQMCQKKHSHSHHLKAAIVVVLVSTKSACKARWFSEKEAEELYSLANEIGSDF 120
           HGLP LLKQM QKK  HSH LKAA++V+++STK+ACK RWFSEKEAE+LYSLANEIGSDF
Sbjct: 61  HGLPNLLKQMYQKK--HSHRLKAAVMVLMISTKNACKVRWFSEKEAEDLYSLANEIGSDF 120

Query: 121 FGDSNIGQSNSLTAITTVMERFFPHLKLGQIVASMEVKPGYGVFTIHFNISKTMQYTPQE 180
           FGD+N G +NSLT IT VMERFFPHLKLGQIVA+MEVKPGYGVF   FNISKTMQ++ Q+
Sbjct: 121 FGDTNTGPNNSLTTITAVMERFFPHLKLGQIVAAMEVKPGYGVFATDFNISKTMQFSQQD 180

Query: 181 KIRLFVAQKDNTETSACIVSPPQVNFLVNGIGVNGRTISYMDTGPQLPTNVTHLLKLGSN 240
           KI LFVAQKDNTETSACI+SPPQVNFLVNG GVNGRT  +MDTGPQLPTNVTH+LKLG+N
Sbjct: 181 KILLFVAQKDNTETSACIISPPQVNFLVNGKGVNGRTNIFMDTGPQLPTNVTHMLKLGAN 240

Query: 241 LFQAIGSFNGHYVIAVAIMGTAPLPDSSVLQDHVQPVVSTVDSDSDIIEGPSRISLNCPI 300
           L QAIG+FNGHYVIAVAIMGTAPLPDSSVLQD+VQPVVSTVDSDSDIIEGPSRISLNCPI
Sbjct: 241 LLQAIGNFNGHYVIAVAIMGTAPLPDSSVLQDYVQPVVSTVDSDSDIIEGPSRISLNCPI 300

Query: 301 SYTRIKVPVKGRSCKHLQ----------CFDFYNFIDINSRRPSWRCPHCNQYICFLDIR 360
           SYTRIKVPVK RSCKHLQ          CFDFYNFI INSRRPSWRCPHCNQYICFLDIR
Sbjct: 301 SYTRIKVPVKSRSCKHLQVSISCHGIWKCFDFYNFIGINSRRPSWRCPHCNQYICFLDIR 360

Query: 361 VDQNMLKVIKEVAENVTEVIISADGSWKAILENDDRNGQSLDDSLNHQNEMEQQESTTAP 420
           VDQNM+KVI+EVAENVTEVIISADGSWKAILEND+ +G+ LDDSLN QNE +Q+  +T P
Sbjct: 361 VDQNMMKVIREVAENVTEVIISADGSWKAILENDNGDGRPLDDSLNLQNERDQE--STVP 420

Query: 421 DVVDLTQVDDDMDICDLEIEDTKPCLGNQNQLLSSGLNTPSEMNSNSSNPNFAAVLDDDF 480
           DV+DL +VDDD++ICDLEIED KPCLGN+                     NFAAVLDDDF
Sbjct: 421 DVLDLIEVDDDINICDLEIEDEKPCLGNK---------------------NFAAVLDDDF 480

Query: 481 WSGIETGEILTSSTRSD--------APNFTGLMQLAGLTDPAIPAL-NHVVGVPGPVTFS 540
           WSGI+T  ILTSS R+D        APNF GLMQ A LT+P  P L NH  GVPG V F 
Sbjct: 481 WSGIDTDRILTSSARTDAPIGNNPPAPNFAGLMQSAVLTNPVTPVLNNHGAGVPGHVIFL 540

Query: 541 SPALYDQNNLQIHVLTSNDNNQYGRMASMVRPSFTVSSRTPTAVQALPAQSQASGQPYGS 600
           SPALYDQNNLQ   L SN+N +YGR  S+ RP     SR PT  QALP  SQASGQ Y S
Sbjct: 541 SPALYDQNNLQTQALNSNENTEYGRTTSIARP----LSRMPTTAQALPYPSQASGQQYSS 600

Query: 601 RTPTISSAPQVGQNIPINRDGLNTISLDSERRQQLPRHPGDSPHATNPSPFHHPQTMQNR 660
           RT TISSA QVG +IP NRDGLNTIS DSER QQ PRHPGDS HATN +PFHHP T QNR
Sbjct: 601 RTTTISSASQVGPSIPTNRDGLNTISRDSERSQQFPRHPGDSHHATNLAPFHHPPTSQNR 660

Query: 661 DPQDRSFTHGQSVQASTALRPSLGILTDFQNPHLQQALNFRMSNFRNPIPS-NARPSSPF 720
           DP   SFT GQSVQASTALRPS  +LTDFQNPHLQQALN RMS  RN  PS N RPS PF
Sbjct: 661 DP--HSFTPGQSVQASTALRPSTRLLTDFQNPHLQQALNLRMSQLRNQNPSNNVRPSLPF 720

Query: 721 SRPMSQV-SGYGGSAYAAVT--SQHARMMAISQRAELMRQSSAMPLQNQTPRSAHSLQTT 780
           SR MSQV  GY G +YAAVT  SQ+ARM+A SQRAELMRQSSAM LQNQT RSAHSLQTT
Sbjct: 721 SRAMSQVGGGYSGPSYAAVTPNSQNARMVA-SQRAELMRQSSAMSLQNQTFRSAHSLQTT 780

Query: 781 PDGLGI-SAGDLRHVGGMSQSVTTAAGSVDLTSEQNWQPSGRMRGSLSGRAYSDAYGHLI 840
           PDGL + +AG+LR+VGGMSQSVT AAG VD +SEQNWQPSGRMRGSLSGRA+SDA+GHLI
Sbjct: 781 PDGLRMPAAGELRNVGGMSQSVTLAAGLVDPSSEQNWQPSGRMRGSLSGRAFSDAHGHLI 837

Query: 841 IQPTQSSQSAQPPSNSTPTQPGALFTQAQ 846
           I PTQS QSA+PPSN TPTQP A  TQAQ
Sbjct: 841 IHPTQSVQSARPPSNPTPTQPSAPSTQAQ 837

BLAST of Sed0011553 vs. ExPASy TrEMBL
Match: A0A6J1ESZ6 (E4 SUMO-protein ligase PIAL2-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111435722 PE=4 SV=1)

HSP 1 Score: 1219.9 bits (3155), Expect = 0.0e+00
Identity = 642/856 (75.00%), Postives = 706/856 (82.48%), Query Frame = 0

Query: 1   MGAPSPYDLTMNRISLYIDGLTSHVNRIDQIDPAHFCSLCFSLARCIDFAIASNFVPSKV 60
           MGA +PY++ ++RIS YID LT +VNR+DQIDP   C++CFSLAR IDFAIA++FVPSK 
Sbjct: 1   MGATTPYEMKLDRISSYIDSLTLYVNRVDQIDPVQLCNICFSLARSIDFAIANDFVPSKA 60

Query: 61  HGLPTLLKQMCQKKHSHSHHLKAAIVVVLVSTKSACKARWFSEKEAEELYSLANEIGSDF 120
            GLP+LLKQ+CQKK  HSHHLKAAI+V++++ K+ACK +WFSEKEAEELYSLANEIGSDF
Sbjct: 61  QGLPSLLKQICQKK--HSHHLKAAIMVLMIAAKNACKVKWFSEKEAEELYSLANEIGSDF 120

Query: 121 FGDSNIGQSNSLTAITTVMERFFPHLKLGQIVASMEVKPGYGVFTIHFNISKTMQYTPQE 180
           F D+N G SNSLT ITTVMERFFP LKLGQIV S EVKPGYGVF   FNISKT+QY PQE
Sbjct: 121 FVDTNTGPSNSLTTITTVMERFFPRLKLGQIVISAEVKPGYGVFAFDFNISKTIQYAPQE 180

Query: 181 KIRLFVAQKDNTETSACIVSPPQVNFLVNGIGVNGRTISYMDTGPQLPTNVTHLLKLGSN 240
           KIRLFVAQKDNTETSACI+SPPQVNFLVNG GVNGRT  YMDTGPQLPTNVTH+LKLGSN
Sbjct: 181 KIRLFVAQKDNTETSACIISPPQVNFLVNGRGVNGRTNIYMDTGPQLPTNVTHMLKLGSN 240

Query: 241 LFQAIGSFNGHYVIAVAIMGTAPLPDSSVLQDHVQPVVSTVDSDSDIIEGPSRISLNCPI 300
           L Q IGSFNGHYVIAVA+MG+AP PDSSVLQDH QPVVSTVDSDSDIIEGPSRISLNCPI
Sbjct: 241 LLQVIGSFNGHYVIAVAVMGSAPSPDSSVLQDHEQPVVSTVDSDSDIIEGPSRISLNCPI 300

Query: 301 SYTRIKVPVKGRSCKHLQCFDFYNFIDINSRRPSWRCPHCNQYICFLDIRVDQNMLKVIK 360
           SYTRIKVPVKGRSCKHLQCFDFYNFIDINSRRPSWRCPHCNQYICFLDI +DQNMLKVI+
Sbjct: 301 SYTRIKVPVKGRSCKHLQCFDFYNFIDINSRRPSWRCPHCNQYICFLDICIDQNMLKVIR 360

Query: 361 EVAENVTEVIISADGSWKAILENDDRNGQSLDDSLNHQNEMEQQESTTAPDVVDLTQVDD 420
           EVAENVTEVIISADGSWKAILEND  +G+ LDDSLN QNE  QQEST  PDV+DLT+VDD
Sbjct: 361 EVAENVTEVIISADGSWKAILENDCGDGRPLDDSLNQQNERAQQESTAPPDVLDLTEVDD 420

Query: 421 DMDICDLEIEDTKPCLGNQNQLLSSGLNTPSEMNSNSSNPNFAAVLDDDFWSGIETGEIL 480
           DM+IC+LE ED KPCLGN+NQ +SS LN  S MN NS N NF+A LDDDFWSG+ T  +L
Sbjct: 421 DMNICNLETEDRKPCLGNKNQPVSSSLNILSGMNRNSLNQNFSAALDDDFWSGMVTDRLL 480

Query: 481 TSSTRSD--------APNFTGLMQLAGLTDPAIPALNHVVGVPGPVTFSSPALYDQNNLQ 540
            SS RSD        AP+F GL Q AGLTD   P LNH VGVPG V F  PA YDQNN+Q
Sbjct: 481 ISSIRSDAPMGSSTAAPSFAGLTQSAGLTDAVSPVLNHDVGVPGQVNFPFPAFYDQNNVQ 540

Query: 541 IHVLTSNDNNQYGRMASMVRPSFTVSSRTPTAVQALPAQSQASGQPYGSRTPTISSAPQV 600
           + V  SN++NQYGRM S+ RP     SRT  A Q LPAQSQ SGQ Y SRT TISSAPQV
Sbjct: 541 VQVSNSNESNQYGRMTSIARP----VSRT-LAGQVLPAQSQTSGQQYSSRTSTISSAPQV 600

Query: 601 GQNIPINRDGLNTISLDSERRQQLPRHPGDSPHATNPSPFHHPQTMQNRDPQDRSFTHGQ 660
           GQ+IPI+RDGLNTIS DSERRQ  PRH GD  HATN +PF  P  +QNR+PQDRSFT GQ
Sbjct: 601 GQSIPISRDGLNTISRDSERRQPFPRHHGDLHHATNLAPFLRPPIVQNREPQDRSFTPGQ 660

Query: 661 SVQASTALRPSLGILTDFQNPHLQQALNFRMSNFRNPIPSNARPSSPFSRPMSQV-SGYG 720
           SV+ASTA RPS GILTDFQNPHLQQ+LN R+S+ RN  PS+ RPS PFSRP SQV  GYG
Sbjct: 661 SVRASTAQRPSAGILTDFQNPHLQQSLNLRISHLRNQNPSSVRPSLPFSRPTSQVGGGYG 720

Query: 721 GSAYAAVT--SQHARMMAISQRAELMRQSSAMPLQNQTPRSAHSLQTTPDGLGISAGDLR 780
           GSAY AVT  +QHARMM  SQRAE+MRQSSAM LQNQT RS H LQTTPDGL   AGDLR
Sbjct: 721 GSAYPAVTPHNQHARMMVASQRAEMMRQSSAMSLQNQTSRSPHPLQTTPDGLRRPAGDLR 780

Query: 781 HVGGMSQSVTTAAGSVDLTSEQNWQPSGRMRGSLSGRAYSDAYGHLIIQPTQSSQSAQPP 840
           +VGGM+QSVT A+  +D + EQN QP GRMRGSLSGRAYSDAYG +IIQPTQ  QS +PP
Sbjct: 781 NVGGMTQSVTMASDLLDPSVEQNRQPIGRMRGSLSGRAYSDAYG-VIIQPTQPVQSTRPP 840

Query: 841 SNSTPTQPGALFTQAQ 846
           SN T TQ  A  T AQ
Sbjct: 841 SNLTTTQSNAPSTHAQ 848

BLAST of Sed0011553 vs. ExPASy TrEMBL
Match: A0A6J1H5H8 (E4 SUMO-protein ligase PIAL2-like isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111459766 PE=4 SV=1)

HSP 1 Score: 1218.0 bits (3150), Expect = 0.0e+00
Identity = 652/862 (75.64%), Postives = 710/862 (82.37%), Query Frame = 0

Query: 1   MGAPSPYDLTMNRISLYIDGLTSHVNRIDQIDPAHFCSLCFSLARCIDFAIASNFVPSKV 60
           MG P PY++ +NRISLYIDG+TS VNR DQIDPA+FC+LCFSLARCIDFAIA+NFVPS V
Sbjct: 1   MGTPLPYEMYLNRISLYIDGITSLVNRNDQIDPAYFCNLCFSLARCIDFAIANNFVPSNV 60

Query: 61  HGLPTLLKQMCQKKHSHSHHLKAAIVVVLVSTKSACKARWFSEKEAEELYSLANEIGSDF 120
           HGLP+LLKQM QKK  HSH LKAA++V+++STK+ACK RWFSEKEAEELYSLANEIGSDF
Sbjct: 61  HGLPSLLKQMYQKK--HSHRLKAAVMVLMISTKNACKVRWFSEKEAEELYSLANEIGSDF 120

Query: 121 FGDSNIGQSNSLTAITTVMERFFPHLKLGQIVASMEVKPGYGVFTIHFNISKTMQYTPQE 180
           FGD+N G +NSLT IT VMERFFPHLKLGQIVA MEVKPGYGVF   FNISKTMQ + QE
Sbjct: 121 FGDTNTGPNNSLTTITAVMERFFPHLKLGQIVADMEVKPGYGVFATDFNISKTMQLSQQE 180

Query: 181 KIRLFVAQKDNTETSACIVSPPQVNFLVNGIGVNGRTISYMDTGPQLPTNVTHLLKLGSN 240
           KI LFVAQKDNTETSACI+SPPQVNFLVNG GVNGRT  +MDTGPQLPTNVTH+LKLG+N
Sbjct: 181 KILLFVAQKDNTETSACIISPPQVNFLVNGKGVNGRTNIFMDTGPQLPTNVTHMLKLGAN 240

Query: 241 LFQAIGSFNGHYVIAVAIMGTAPLPDSSVLQDHVQPVVSTVDSDSDIIEGPSRISLNCPI 300
           L QAIG+FNGHYVIAVAIMGTAPLPDSSVLQD+VQ VVSTVD DSDIIEGPSRISLNCPI
Sbjct: 241 LLQAIGNFNGHYVIAVAIMGTAPLPDSSVLQDYVQTVVSTVDPDSDIIEGPSRISLNCPI 300

Query: 301 SYTRIKVPVKGRSCKHLQCFDFYNFIDINSRRPSWRCPHCNQYICFLDIRVDQNMLK--- 360
           SYTRIKVPVK RSCKHLQCFDFYNFI INSRRPSWRCPHCNQYICFLDIRVDQNMLK   
Sbjct: 301 SYTRIKVPVKSRSCKHLQCFDFYNFIGINSRRPSWRCPHCNQYICFLDIRVDQNMLKASI 360

Query: 361 -VIKEVAENVTEVIISADGSWKAILENDDRNGQSLDDSLNHQNEMEQQESTTAPDVVDLT 420
            VI+EVAENVTEVIISADGSWKAIL+N D  G+ LDDSLN QNE +Q+  +T P V+DL 
Sbjct: 361 VVIREVAENVTEVIISADGSWKAILDNGD--GRPLDDSLNLQNERDQE--STVPVVLDLI 420

Query: 421 QVDDDMDICDLEIEDTKPCLGNQNQLLSSGLNTPSEMNSNSSNPNFAAVLDDDFWSGIET 480
           +VDDD++ICDLEIED KPC+GN+                     NFAAVLDDDFWSGI+T
Sbjct: 421 EVDDDINICDLEIEDEKPCIGNK---------------------NFAAVLDDDFWSGIDT 480

Query: 481 GEILTSSTRSD--------APNFTGLMQLAGLTDPAIPAL-NHVVGVPGPVTFSSPALYD 540
             ILTSS R+D        APNF GLMQ A LT+P  P L NH  GVPG V FSSPALYD
Sbjct: 481 DRILTSSARTDAPIGNNPPAPNFAGLMQSAVLTNPVTPVLNNHGAGVPGHVIFSSPALYD 540

Query: 541 QNNLQIHVLTSNDNNQYGRMASMVRPSFTVSSRTPTAVQALPAQSQASGQPYGSRTPTIS 600
           QNNLQ   L SN+N++YGR  S+ RP     SR PT  QALP+ SQASGQ Y SRT TIS
Sbjct: 541 QNNLQSQALNSNENSEYGRTTSIARP----LSRMPTTAQALPSLSQASGQQYSSRTTTIS 600

Query: 601 SAPQVGQNIPINRDGLNTISLDSERRQQLPRHPGDSPHATNPSPFHHPQTMQNRDPQDRS 660
           SAPQVG +IP NRDGLNTIS DSER QQ PR PGDS HATN +PFHHP T+QN DP D S
Sbjct: 601 SAPQVGPSIPTNRDGLNTISRDSERSQQFPRRPGDSHHATNLAPFHHPPTLQNWDPLDHS 660

Query: 661 FTHGQSVQASTALRPSLGILTDFQNPHLQQALNFRMSNFRNPIPS-NARPSSPFSRPMSQ 720
           FT GQSVQASTALRPS G+LTDFQNPHLQQALN RMS  RN  PS N RPS PFSR MSQ
Sbjct: 661 FTPGQSVQASTALRPSRGLLTDFQNPHLQQALNLRMSQLRNQNPSNNVRPSLPFSRAMSQ 720

Query: 721 V-SGYGGSAYAAVT--SQHARMMAISQRAELMRQSSAMPLQNQTPRSAHSLQTTPDGLGI 780
           V  GY G +YAAVT  SQ+ARMM  SQRAELMRQSSAM LQNQT RSAHSLQTTPDGL +
Sbjct: 721 VGGGYSGPSYAAVTPNSQNARMMVASQRAELMRQSSAMSLQNQTFRSAHSLQTTPDGLRM 780

Query: 781 -SAGDLRHVGGMSQSVTTAAGSVDLTSEQNWQPSGRMRGSLSGRAYSDAYGHLIIQPTQS 840
            +AG+LR+VGGMSQSVT AAG V+ +SEQNWQPSGRMRGSLSGRA+SDA+GHLII PTQ 
Sbjct: 781 PTAGELRNVGGMSQSVTPAAGLVEPSSEQNWQPSGRMRGSLSGRAFSDAHGHLIIHPTQP 831

Query: 841 SQSAQPPSNSTPTQPGALFTQA 845
            QSA+PPSNSTPTQP A  TQA
Sbjct: 841 VQSARPPSNSTPTQPSAPSTQA 831

BLAST of Sed0011553 vs. TAIR 10
Match: AT4G23560.1 (glycosyl hydrolase 9B15 )

HSP 1 Score: 572.0 bits (1473), Expect = 1.1e-162
Identity = 271/395 (68.61%), Postives = 329/395 (83.29%), Query Frame = 0

Query: 837  PGALFTQAQSWTVVEYEKEISSVNQLEHLRNSIRWGADFILRAHVSATTLYTQVGDGNGD 896
            P +  T   SW  +EY+ EISSVNQL +LR++I+WG DFILRAH S   LYTQVGDGN D
Sbjct: 83   PMSFTTTLLSWAAIEYQNEISSVNQLGYLRSTIKWGTDFILRAHTSPNMLYTQVGDGNSD 142

Query: 897  HQCWERPEDMDTPRTLYKITSASPGTEAAAEAAAALAATSIVFNHVDTNYSRTLLQHSIS 956
            H CWERPEDMDT RTLY I+S+SPG+EAA EAAAALAA S+VF  VD+ YS TLL H+ +
Sbjct: 143  HSCWERPEDMDTSRTLYSISSSSPGSEAAGEAAAALAAASLVFKSVDSTYSSTLLNHAKT 202

Query: 957  LFQFADQFRGSYSASCPFYCSFSGYQDELLWAAAWLYKATGTNKYLSYVISNQGWSQAVS 1016
            LF+FAD++RGSY ASCPFYCS+SGYQDELLWAAAWLYKATG   Y++YVISN+ WSQAV+
Sbjct: 203  LFEFADKYRGSYQASCPFYCSYSGYQDELLWAAAWLYKATGDKIYINYVISNKDWSQAVN 262

Query: 1017 EFSWDNKFVGAQTLLAKEFYKGMKELGKFKSDVESFICALMPGSGSTQIRTTPGGLLFVR 1076
            EFSWDNKFVGAQ LL  EFY G  +L KFKSDVESF+CA+MPGS S QI+ TPGGLLF+R
Sbjct: 263  EFSWDNKFVGAQALLVSEFYNGANDLAKFKSDVESFVCAMMPGSSSQQIKPTPGGLLFIR 322

Query: 1077 DSSNLQYVSSSSMVLFMYSKLLNQARVDGVHCRSKIFSSSQIKSFAKSQVDYILGNNPLK 1136
            DSSNLQYV++++ VLF YSK L +A V  + C S  F+ SQI++FAKSQVDYILGNNP+K
Sbjct: 323  DSSNLQYVTTATTVLFHYSKTLTKAGVGSIQCGSTKFTVSQIRNFAKSQVDYILGNNPMK 382

Query: 1137 MSYMVGFGSKYPLQLHHRASSIPSIRTYVTKVGCSDGYSKYYNSNNPNPNIHVGAIVGGP 1196
            MSYMVGFG+KYP Q HHR SS+PSI++   K+ C+ GYS YYNS+ PNPN+H+GAIVGGP
Sbjct: 383  MSYMVGFGTKYPTQPHHRGSSLPSIQSKPEKIDCNGGYS-YYNSDTPNPNVHIGAIVGGP 442

Query: 1197 DSNDRFSDTRSDHTHSEPTTYMNAAFVGSVAALVA 1232
            +S+D++SD +SD++H+EPTTY+NAAF+G VAAL++
Sbjct: 443  NSSDQYSDKKSDYSHAEPTTYINAAFIGPVAALIS 476

BLAST of Sed0011553 vs. TAIR 10
Match: AT4G09740.1 (glycosyl hydrolase 9B14 )

HSP 1 Score: 557.4 bits (1435), Expect = 2.9e-158
Identity = 264/395 (66.84%), Postives = 325/395 (82.28%), Query Frame = 0

Query: 837  PGALFTQAQSWTVVEYEKEISSVNQLEHLRNSIRWGADFILRAHVSATTLYTQVGDGNGD 896
            P +  T   SW  +EY+ EI+ VNQL +LR++I+WG +FILRAH S   LYTQVGDGN D
Sbjct: 83   PMSFTTTLLSWAALEYQNEITFVNQLGYLRSTIKWGTNFILRAHTSTNMLYTQVGDGNSD 142

Query: 897  HQCWERPEDMDTPRTLYKITSASPGTEAAAEAAAALAATSIVFNHVDTNYSRTLLQHSIS 956
            H CWERPEDMDTPRTLY I+S+SPG+EAA EAAAALAA S+VF  VD+ YS  LL ++ S
Sbjct: 143  HSCWERPEDMDTPRTLYSISSSSPGSEAAGEAAAALAAASLVFKLVDSTYSSKLLNNAKS 202

Query: 957  LFQFADQFRGSYSASCPFYCSFSGYQDELLWAAAWLYKATGTNKYLSYVISNQGWSQAVS 1016
            LF+FAD++RGSY ASCPFYCS SGYQDELLWAAAWLYKATG   YL+YVISN+ WS+A++
Sbjct: 203  LFEFADKYRGSYQASCPFYCSHSGYQDELLWAAAWLYKATGEKSYLNYVISNKDWSKAIN 262

Query: 1017 EFSWDNKFVGAQTLLAKEFYKGMKELGKFKSDVESFICALMPGSGSTQIRTTPGGLLFVR 1076
            EFSWDNKF G Q LLA EFY G  +L KFK+DVESF+CALMPGS S QI+ TPGG+LF+R
Sbjct: 263  EFSWDNKFAGVQALLASEFYNGANDLEKFKTDVESFVCALMPGSSSQQIKPTPGGILFIR 322

Query: 1077 DSSNLQYVSSSSMVLFMYSKLLNQARVDGVHCRSKIFSSSQIKSFAKSQVDYILGNNPLK 1136
            DSSNLQYV++++ +LF YSK L +A V  + C S  F+ SQI++FAKSQVDYILGNNPLK
Sbjct: 323  DSSNLQYVTTATTILFYYSKTLTKAGVGSIQCGSTQFTVSQIRNFAKSQVDYILGNNPLK 382

Query: 1137 MSYMVGFGSKYPLQLHHRASSIPSIRTYVTKVGCSDGYSKYYNSNNPNPNIHVGAIVGGP 1196
            MSYMVGFG+KYP Q HHR SS+PSI++   K+ C+ G+S YYN + PNPN+H GAIVGGP
Sbjct: 383  MSYMVGFGTKYPTQPHHRGSSLPSIQSKPEKIDCNGGFS-YYNFDTPNPNVHTGAIVGGP 442

Query: 1197 DSNDRFSDTRSDHTHSEPTTYMNAAFVGSVAALVA 1232
            +S+D++SD R+D++H+EPTTY+NAAF+GSVAAL++
Sbjct: 443  NSSDQYSDKRTDYSHAEPTTYINAAFIGSVAALIS 476

BLAST of Sed0011553 vs. TAIR 10
Match: AT1G02800.1 (cellulase 2 )

HSP 1 Score: 403.3 bits (1035), Expect = 7.1e-112
Identity = 206/402 (51.24%), Postives = 280/402 (69.65%), Query Frame = 0

Query: 837  PGALFTQAQSWTVVEYEKEISSVNQLEHLRNSIRWGADFILRAHVSATTLYTQVGDGNGD 896
            P A  T   SW+++E+   + S  +L + +++IRW  DF+L+A     T+Y QVGD N D
Sbjct: 103  PMAFTTTMLSWSLIEFGGLMKS--ELPNAKDAIRWATDFLLKATSHPDTIYVQVGDPNMD 162

Query: 897  HQCWERPEDMDTPRTLYKITSASPGTEAAAEAAAALAATSIVFNHVDTNYSRTLLQHSIS 956
            H CWERPEDMDTPR+++K+   +PG++ A E AAALAA SIVF   D +YS  LLQ +I+
Sbjct: 163  HACWERPEDMDTPRSVFKVDKNNPGSDIAGEIAAALAAASIVFRKCDPSYSNHLLQRAIT 222

Query: 957  LFQFADQFRGSYSAS-----CPFYCSFSGYQDELLWAAAWLYKATGTNKYLSYVISN--- 1016
            +F FAD++RG YSA      CPFYCS+SGYQDELLW AAWL KAT    YL+Y+ +N   
Sbjct: 223  VFTFADKYRGPYSAGLAPEVCPFYCSYSGYQDELLWGAAWLQKATNNPTYLNYIKANGQI 282

Query: 1017 QGWSQAVSEFSWDNKFVGAQTLLAKEF-YKGMKELGKFKSDVESFICALMPGSGSTQIRT 1076
             G  +  + FSWDNK VGA+ LL+KEF  + +K L ++K   +SFIC+++PG+ S+Q   
Sbjct: 283  LGADEFDNMFSWDNKHVGARILLSKEFLIQKVKSLEEYKEHADSFICSVLPGASSSQY-- 342

Query: 1077 TPGGLLFVRDSSNLQYVSSSSMVLFMYSKLLNQARVDGVHCRSKIFSSSQIKSFAKSQVD 1136
            TPGGLLF    SN+QYV+S+S +L  Y+K L  AR    +C   + + ++++S AK QVD
Sbjct: 343  TPGGLLFKMGESNMQYVTSTSFLLLTYAKYLTSART-VAYCGGSVVTPARLRSIAKKQVD 402

Query: 1137 YILGNNPLKMSYMVGFGSKYPLQLHHRASSIPSIRTYVTKVGCSDGYSKYYNSNNPNPNI 1196
            Y+LG NPLKMSYMVG+G KYP ++HHR SS+PS+  + T++ C DG+S  + S +PNPN 
Sbjct: 403  YLLGGNPLKMSYMVGYGLKYPRRIHHRGSSLPSVAVHPTRIQCHDGFS-LFTSQSPNPND 462

Query: 1197 HVGAIVGGPDSNDRFSDTRSDHTHSEPTTYMNAAFVGSVAAL 1230
             VGA+VGGPD ND+F D RSD+  SEP TY+NA  VG++A L
Sbjct: 463  LVGAVVGGPDQNDQFPDERSDYGRSEPATYINAPLVGALAYL 498

BLAST of Sed0011553 vs. TAIR 10
Match: AT5G41580.1 (RING/U-box superfamily protein )

HSP 1 Score: 402.5 bits (1033), Expect = 1.2e-111
Identity = 245/605 (40.50%), Postives = 352/605 (58.18%), Query Frame = 0

Query: 13  RISLYIDGLTSHVNRIDQIDPAHFCSLCFSLARCIDFAIASNFVPSKVHGLPTLLKQMCQ 72
           R++     L  H+    ++DP  F   C S A+ IDFAIA+N +P KV   P LLKQ+C 
Sbjct: 28  RLASVTQRLRYHIQDGAKVDPKEFQICCISFAKGIDFAIANNDIPKKVEEFPWLLKQLC- 87

Query: 73  KKHSHSHHLKAAIVVVLVSTKSACKARWFSEKEAEELYSLANEIGSDF--FGDSNIGQSN 132
            +H    + K A++V+++S K AC   WFS+ E++EL +LA+EI + F   G ++ G  +
Sbjct: 88  -RHGTDVYTKTALMVLMISVKHACHLGWFSDSESQELIALADEIRTCFGSSGSTSPGIKS 147

Query: 133 SLTAITTVMERFFPHLKLGQIVASMEVKPGYGVFTIHFNISKTMQYTPQEKIRLFVAQKD 192
             +  + +MERF+P +KLG ++ S EVK GY +    F ISK M ++ QEKIRLFVAQ D
Sbjct: 148 PGSTFSQIMERFYPFVKLGHVLVSFEVKAGYTMLAHDFYISKNMPHSLQEKIRLFVAQTD 207

Query: 193 NTETSACIVSPPQVNFLVNGIGVNGRTISYMDTGPQLPTNVTHLLKLGSNLFQAIGSFNG 252
           N +TSACI +PP+V+FL+NG GV  R    MDTGPQLPTNVT  LK G+NL Q +G+F G
Sbjct: 208 NIDTSACISNPPEVSFLLNGKGVEKRVNIAMDTGPQLPTNVTAQLKYGTNLLQVMGNFKG 267

Query: 253 HYVIAVAIMGTAPLPDSSVLQDHVQPVVSTVDSDSDIIEGPSRISLNCPISYTRIKVPVK 312
           +Y+I +A  G    P+  VL+D++Q  V     DSDIIEGPSR+SL+CPIS  RIK+PVK
Sbjct: 268 NYIIIIAFTGLVVPPEKPVLKDYLQSGVIEASPDSDIIEGPSRVSLSCPISRKRIKLPVK 327

Query: 313 GRSCKHLQCFDFYNFIDINSRRPSWRCPHCNQYICFLDIRVDQNMLKVIKEVAENVTEVI 372
           G+ CKHLQCFDF N++ IN R P+WRCPHCNQ +C+ DIR+DQNM K++K+V  N  +VI
Sbjct: 328 GQLCKHLQCFDFSNYVHINMRNPTWRCPHCNQPVCYPDIRLDQNMAKILKDVEHNAADVI 387

Query: 373 ISADGSWKAILENDDRNGQSLDDSLNHQNEMEQQESTTAPDVVDLTQVDDDMDI---CDL 432
           I A G+WK + +N     + + + + H  E       + P V DLT  DDD ++    D 
Sbjct: 388 IDAGGTWK-VTKNTGETPEPVREII-HDLEDPMSLLNSGPVVFDLTG-DDDAELEVFGDN 447

Query: 433 EIEDTKPCLGNQNQLLSSGLNTPSEMNSNSSNPNFAAVLDDDFWSGIETGEILT------ 492
           ++ED KPC+ +     + G +  +  N + SN +++++ D      ++  EIL+      
Sbjct: 448 KVEDRKPCMSD-----AQGQSNNNNTNKHPSNDDYSSIFDISDVIALDP-EILSALGNTA 507

Query: 493 -----SSTRSDAPNFTGLMQLAGLTDPAIPALNHVVGVPGPVTFS-SPALYDQNNLQIHV 552
                +S       ++ L Q+    DP          +P PV FS +P+  D+      V
Sbjct: 508 PQPHQASNTGTGQQYSNLSQIPMSIDP----------MPVPVPFSQTPSPRDRPATTSTV 567

Query: 553 LT-SNDNNQYGRM-ASMVRPSFTVSSRT--PTAVQALPAQSQASGQPYGSRTPTISSAPQ 597
            T  N + QY ++ AS V P+ T   RT  P   Q   +Q+     PY SR  ++    Q
Sbjct: 568 FTIPNPSPQYSQVHASPVTPTGTYLGRTTSPRWNQTYQSQAPPMTTPYTSRKVSVPVTSQ 611

HSP 2 Score: 38.1 bits (87), Expect = 6.0e-02
Identity = 53/208 (25.48%), Postives = 87/208 (41.83%), Query Frame = 0

Query: 672 NPHLQQALNFRMSNFRNPIPS----NARPSSPFSRPMSQVSG------YGGSAYAAVTSQ 731
           +P  + A    +    NP P     +A P +P    + + +       Y   A    T  
Sbjct: 535 SPRDRPATTSTVFTIPNPSPQYSQVHASPVTPTGTYLGRTTSPRWNQTYQSQAPPMTTPY 594

Query: 732 HARMMAISQRAELMRQSSAMPLQNQTPRSAHSLQTTPDGLGISAGDLRHV--------GG 791
            +R +++   ++     S+       PR    + + P+  G+      H         G 
Sbjct: 595 TSRKVSVPVTSQSPANVSSFVQSQHVPR----VLSQPNNYGVRGLTSSHASTSRQHPSGP 654

Query: 792 MSQSVTTAAGSVD----LTSEQNWQPSGRMRGSLSGRAYSDAYGHLIIQPTQSSQSAQPP 851
             QSV+  +  VD    +    NW+P  RMRGSL   ++S A  H+II+P+Q SQ++   
Sbjct: 655 TVQSVSRLSDLVDVDLTVPDTSNWRP--RMRGSLVPGSHSTALDHMIIRPSQQSQTSTRL 714

Query: 852 SNSTPTQ-PGALFTQAQS-WTVVEYEKE 856
           ++S P Q P    +QAQS +T   Y  E
Sbjct: 715 NSSQPVQTPSVQTSQAQSPFTTAAYRTE 736

BLAST of Sed0011553 vs. TAIR 10
Match: AT4G02290.1 (glycosyl hydrolase 9B13 )

HSP 1 Score: 390.6 bits (1002), Expect = 4.7e-108
Identity = 194/400 (48.50%), Postives = 276/400 (69.00%), Query Frame = 0

Query: 837  PGALFTQAQSWTVVEYEKEISSVNQLEHLRNSIRWGADFILRAHVSATTLYTQVGDGNGD 896
            P A  T   SW+V+E+   + S  +L++ + +IRW  D++L+A     T+Y QVGD N D
Sbjct: 112  PMAFTTTMLSWSVIEFGGLMKS--ELQNAKIAIRWATDYLLKATSQPDTIYVQVGDANKD 171

Query: 897  HQCWERPEDMDTPRTLYKITSASPGTEAAAEAAAALAATSIVFNHVDTNYSRTLLQHSIS 956
            H CWERPEDMDT R+++K+    PG++ AAE AAALAA +IVF   D +YS+ LL+ +IS
Sbjct: 172  HSCWERPEDMDTVRSVFKVDKNIPGSDVAAETAAALAAAAIVFRKSDPSYSKVLLKRAIS 231

Query: 957  LFQFADQFRGSYSAS-----CPFYCSFSGYQDELLWAAAWLYKATGTNKYLSYVISN--- 1016
            +F FAD++RG+YSA      CPFYCS+SGYQDELLW AAWL KAT   KYL+Y+  N   
Sbjct: 232  VFAFADKYRGTYSAGLKPDVCPFYCSYSGYQDELLWGAAWLQKATKNIKYLNYIKINGQI 291

Query: 1017 QGWSQAVSEFSWDNKFVGAQTLLAKEF-YKGMKELGKFKSDVESFICALMPGSGSTQIRT 1076
             G ++  + F WDNK  GA+ LL K F  + +K L ++K   ++FIC+++PG+  +  + 
Sbjct: 292  LGAAEYDNTFGWDNKHAGARILLTKAFLVQNVKTLHEYKGHADNFICSVIPGAPFSSTQY 351

Query: 1077 TPGGLLFVRDSSNLQYVSSSSMVLFMYSKLLNQARVDGVHCRSKIFSSSQIKSFAKSQVD 1136
            TPGGLLF    +N+QYV+S+S +L  Y+K L  A+   VHC   +++  +++S AK QVD
Sbjct: 352  TPGGLLFKMADANMQYVTSTSFLLLTYAKYLTSAKT-VVHCGGSVYTPGRLRSIAKRQVD 411

Query: 1137 YILGNNPLKMSYMVGFGSKYPLQLHHRASSIPSIRTYVTKVGCSDGYSKYYNSNNPNPNI 1196
            Y+LG+NPL+MSYMVG+G K+P ++HHR SS+P + ++  K+ C  G++   NS +PNPN 
Sbjct: 412  YLLGDNPLRMSYMVGYGPKFPRRIHHRGSSLPCVASHPAKIQCHQGFA-IMNSQSPNPNF 471

Query: 1197 HVGAIVGGPDSNDRFSDTRSDHTHSEPTTYMNAAFVGSVA 1228
             VGA+VGGPD +DRF D RSD+  SEP TY+N+  VG++A
Sbjct: 472  LVGAVVGGPDQHDRFPDERSDYEQSEPATYINSPLVGALA 507