Sed0006346 (gene) Chayote v1

Overview


Name	Sed0006346
Type	gene
Organism	Sechium edule (Chayote v1)
Description	tRNA pseudouridine synthase
Location	LG09: 6686082 .. 6690795 (-)
RNA-Seq Expression	Sed0006346
Synteny	Sed0006346

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GCCCTAAGCAAAGATCCATTTTGAATGTTGAATTGAAACAAAAGACTTGGAAGAGAAGGATGAGGAACTGGGTAGCGGGTTCAACAGTTGTTCCTTCCAATCCCATTTCACCGACCTTCACATCTCCAAATCGCTCATCATCTTCCACAGCTCGCAGATTCTCGGTAATGCCCAATTCTTCTCTAATCTCTCCTCATATTTTCATGGCTCAGTTTTCACCAATCCCATATTGATTCTTCAGAAGAATGCACAGGATTCTTGTTATGTTGCAGAGCCTTATTCCCCTGGCTTTAAATGGCGGTTGCTCATTGCTTATGATGGTACTCCATATGCAGGTCCGGGTTTCTTTTTTGTGGCGAATGTATTCAACCATTTTGTATGCTTGTTCTTGGTTTAGCCTTATATGACGACTCTAATTCCTAATTGGATTAAGCTTTAGTCTGCAACGAGTGTGACAGTACTCGAAAGCTTGGGCTTTGAAGGTGTGCTTGCCTCAAAGTCTCAGTTCGAGTCTTACATTAATTTAAAGATACCTGATACTTCCCGTGTCTCTCTCTTTTTCGATTTCTCCCGATATCTGGCCTAGGGACGGGCTTTGAATGTGTGCTCCCCTTAAAGTTTCAAGTTCCAAACTCACCTGTGACATTAATTTGTAGACACCTCTCATGTCTCCATTCTTTTGATGTTTCCCGCTCCCTGACCCTAGGAAGCACGAACACATCTATTTTGACAATGTATCCGAGTTGTACTCGTGTAAAACCAGTGTTTACAAGGCCATCACCTTTCCTCGCCTATGGCCATCGCCTTGCACATAGGCAGGTATTGCGCCTCTAACATAGCTAAGGCGAGCTCCTTCAATCAGGCCCTCGCATTTCCTCGCCATTTTGCGCTTTGCATCGCCTTCGCGTTAGGCCAAGGCGTGCGCCTTGTTAAAGTTTTATTTATTTATTAATATCATAACTTAATATCCACAAAAAGAACCTATAATTCGTTTATGAAGAAGTTCAACAACTATGTGAAGTCTTTATTAAGAATATTTAATTTTTTAATTGCTATTTCAAATATATGTGTGTTTGTTTATTAGAGTTATTTGATGTAAAATCTTTGTACGTTTAGTTAACTTCAATTTTTTTTTATACAAATAATAATTTAATATTATGGTATATCTTATAATAACATAATTTTTGTGATTTATTATTCTTTTATTTTTTACTTTTTAGTATTTTTCCATATGAATTTATAGTTTTATGAGAATAAACATATGTTTTATTATCAATTTATAAATGCGCCTCTCTTCACTCGGGTCTTGCCTTTTTATCGCCTCTCGCTTTGCTTTGAGGTTATCAAGGAGAGTATCGCCTTGTGTTGTGCCTTGCGCTTTGAAAACACTATGTCCAACACAGATATTTCCGGACAATCAACGGACACGTGTCCGACACTCAAAATTTTGTGTTTTCTTTTATTATTATTATTTTCAATTTCAGACACTTCATGGACACCTCAATTAAAACTTATTTGTTTCTAATGGCGGATGACACGACAATGCGTAGTGTTAGTGGAGGCCGTCAATGACGATGTCTATCGAAGAAAGATGAACTAAAATGAAAAAAGGAAGGAAAGAGAGAAAATAAAATGGAGGGAAGGAAAATGAGATAAAAACTAAAATATTTAGATATTATTATGGGTAGGTTTATAACTAGCGAAAATATTTTGGTTTTCATATTTTAATAGTATATTATATAAAATAATAATAAATCACGTGTCCTCGACGTATCTGTGTTCTGCATTTTTAAATAATGGCGTGTCTACGTGTCCGTATAGTGTCGTGTCCGTATCTTGTGTCCGTGCTTCTTAGTCTTGACCTAGGGACGGACATGGTTATCCCGAGTATAGTAGAGCGAAGCTCCGATTTTCCAGTTAAAAAACTGTATATATGTTTGCAGTAGAGAAAGCCACCCTGAGTAACTAAATAAGGGCTATAGATATAATAGATTATGGTTGGGTTAACCTAGTGTAAATAGAGAATTAAGAAAACAAGATGGAATGGATTTAATCTATAATAGAAAGTTAGCTAAGATACATCTTTTTTCTGTTTGACGATTGAGTTGTGAGATTAGTTGAAGTAAGCTGACTCGGACACTATTAATAAAAATGAGGAATTTGAACCCATAACCTTTTAGTTTGTGGGCACATTTATGCCTGCTGAGCTACTGGGGGGTTTAGAAGAAATATGTTCATTCCTTCGATTTAATGCAATCTTATGACTCTTATTCGCCATTTCAATTGCTATGGGAATACATACTTCTTCCCTTTAGCATTTATATGCTATTTAATTGCATGGATCCCATATCATTCTTTAATGATTCATAAATTATCGACGAACCCAAAAGTTTAAGTTGATAGGTTATGATAAATTTAATTATATCAACCAACACTCTTCTCTACTTGTGGACTTCAAAATTATGTCAATCATGTGGAAATTAACATTAATTAAGGAGAAAACGACTTCAAAGGAGTGTGAATATATGATCTCCCCTATGATTCCATGTTAAATCATGTTAAATTATGTTAAATTACGTCTCCAAGGGGTGGCACAGTGGTTGAAGACTTGGAATTTGAGGGTATGCTTCTCTTGAAGTCCCAGGTTCGAAACTTACCTATGACATTACGTGGTTACCTTATTTCAAAAAAAAAATTATATTAAATTTCCACTAATCCCAAAAGCTTAAGTTGATATGTAGGAGTAAATTTAATTATATCAACTAACATAACAGACAATAAATGGAAAATTTTCATACTATCTAGGACTAGAAAATCTCCCATTGATTTAGCTAGCTTTAAGTTATCTTAAAGATACGTGAATTGGGGTCCGGTCACCGGTGTGAATTATGAGTTAGAGAAGTTTTTTCCATTAGTTTGAATTCCTGCGCTCACTACTTCAGTTTTGATGCTCTCTGTCTTTGTTTCAAATATGTTGATATTAAATCAGGCTGGCAATATCAGCAGTCACCGCCTACCATCCAGTGCCTGATTGAAAAAGCCTTGACTCAAGCAACAAAGGTTGAAAGGAAGGATCTCCGTTTAGTTGGTGCGAGCCGAACAGATACAGGAGTTCATGCCTGGGGACAGGTCTGATGTGCTTTTATGGAGTTGGCTTAAACACATTGTTGGAAAACAATGCTTGGTGTTGCTTACAACAAGTTGAGGTTTCAGGTGGCACACTTTGTTACGCCATTTAGCTTCGATAGCTTGGAAACGGTTCATGCTGCTCTGAATGGTCTTCTTCCTGCGGATATTAGAGTTCGGGAGATTAGTCCTGCTGTTCCCGACTTTCATGCGCGATTTTCTACAATAAGCAAGGTTTATCACTACAAGATATATAATGACACCATCTTGGATCCATTTCAGCGCTTCTACGCTTACCAATGCCTTTATAAACTCAATTCTGCAGTCATGAGAGAAGCTGCGAGTTATTTCATTGGAAAGCATGATTTTTCGGCTTTTGCGAATGCAGCGCAGAATGATCGAGTGGGAAATCCAGTGAAGGTCATTTTTCGGTTTGATGTCATAGAAATGGTAAAGTTTTTAGGTTCTATCTGTCAATGGTTTTTTTTTTCTTTCTATTCGGCTTCTCACGAGTTTAATATGGCTAGTCATGTGTTAATCATTCTGTTTTCAGCTTTATACAAAAACTATGTGGTTAAATGCTTAGCATTCGGTTGTTTTCTTTGTTCAGGGACCTCTAATTCAGCTAGAAGTTGAGGGCTCTGGGTTCTTGTATAGACAAGTTCGGAATATGGTAATTATATTTGTTACACTTTTTTTTAGTCCAAGTTATTTTGTCCGACTTTACTTGGTAAATTCTTGTTGCCACAATGACTTGTATTATGAAATAATTGTAAACAACGACCAATAATAAAGAAAGTGTGAGACAAAGAACACCTCGATTTATGTGGTTCACTAATATTGTATTAGGCCAGCTACATTCACGAGTAGAGGAAAACAACTCTTTTATTTAGGAGATTGTAGGTAAAGAGGATAAAGATTTTATCCAAATTACATAAAAGCCTAATACGAAAATATAAAAACATAGTTAAAATATTTTCTAGGTTTCGGGGTGTTGAACCTTCATCGTGTGCTTCACCTTGGACCCCTCGTAATGTGACATAATTAGTTCAAGGCATTTCGAGAGCTTGTTTTTTTTATAATAAGCTTAGATTCAATTTTCTTAAGTCGGGTTTCATCGTGCAACTGTAAGCATTTCTCCATTACTTTTGCAGGTTGGCTTGTTGATTCAAATTGGAAAGGAAGCAATTTCTCCTGATGTTGTTCCTCAGATTTTGGCTTCTCGAGATCGCAAGGAGCTCGCCAAGTACGCTCCCTTCATTCCCCCTCACGGGCTCTGTCTTGTGAGCATTAAATATAACGAGGACCATCTAAAGCTTCCATCTGGTAGCCCTGGGACTAGTTCTGGTAGGTATCATACTGTGAGCAAATGTAAACTACCTTTTTATTGAATTCATGACAGTTTACAGGTCTGTTCTTACACTTTTGCCAATGACCATGAGCATAGTTTCCTGAGGATGAAATATTAGATTGCTAGTATGATTTAGTTAATTTTGGATTAGTTAGTTTGTAATATATATGAATTAGTATTTTAGTGTTAGGTTGTTATTTGTAGTTTTAATATTGCATAACTTGTATTGAGACTTTG

mRNA sequence

GCCCTAAGCAAAGATCCATTTTGAATGTTGAATTGAAACAAAAGACTTGGAAGAGAAGGATGAGGAACTGGGTAGCGGGTTCAACAGTTGTTCCTTCCAATCCCATTTCACCGACCTTCACATCTCCAAATCGCTCATCATCTTCCACAGCTCGCAGATTCTCGGATTCTTGTTATGTTGCAGAGCCTTATTCCCCTGGCTTTAAATGGCGGTTGCTCATTGCTTATGATGGTACTCCATATGCAGTCACCGCCTACCATCCAGTGCCTGATTGAAAAAGCCTTGACTCAAGCAACAAAGGTTGAAAGGAAGGATCTCCGTTTAGTTGGTGCGAGCCGAACAGATACAGGAGTTCATGCCTGGGGACAGGTGGCACACTTTGTTACGCCATTTAGCTTCGATAGCTTGGAAACGGTTCATGCTGCTCTGAATGGTCTTCTTCCTGCGGATATTAGAGTTCGGGAGATTAGTCCTGCTGTTCCCGACTTTCATGCGCGATTTTCTACAATAAGCAAGGTTTATCACTACAAGATATATAATGACACCATCTTGGATCCATTTCAGCGCTTCTACGCTTACCAATGCCTTTATAAACTCAATTCTGCAGTCATGAGAGAAGCTGCGAGTTATTTCATTGGAAAGCATGATTTTTCGGCTTTTGCGAATGCAGCGCAGAATGATCGAGTGGGAAATCCAGTGAAGGTCATTTTTCGGTTTGATGTCATAGAAATGGGACCTCTAATTCAGCTAGAAGTTGAGGGCTCTGGGTTCTTGTATAGACAAGTTCGGAATATGGTTGGCTTGTTGATTCAAATTGGAAAGGAAGCAATTTCTCCTGATGTTGTTCCTCAGATTTTGGCTTCTCGAGATCGCAAGGAGCTCGCCAAGTACGCTCCCTTCATTCCCCCTCACGGGCTCTGTCTTGTGAGCATTAAATATAACGAGGACCATCTAAAGCTTCCATCTGGTAGCCCTGGGACTAGTTCTGGTAGGTATCATACTGTGAGCAAATGTAAACTACCTTTTTATTGAATTCATGACAGTTTACAGGTCTGTTCTTACACTTTTGCCAATGACCATGAGCATAGTTTCCTGAGGATGAAATATTAGATTGCTAGTATGATTTAGTTAATTTTGGATTAGTTAGTTTGTAATATATATGAATTAGTATTTTAGTGTTAGGTTGTTATTTGTAGTTTTAATATTGCATAACTTGTATTGAGACTTTG

Coding sequence (CDS)

ATGTTGCAGAGCCTTATTCCCCTGGCTTTAAATGGCGGTTGCTCATTGCTTATGATGGTACTCCATATGCAGTCACCGCCTACCATCCAGTGCCTGATTGAAAAAGCCTTGACTCAAGCAACAAAGGTTGAAAGGAAGGATCTCCGTTTAGTTGGTGCGAGCCGAACAGATACAGGAGTTCATGCCTGGGGACAGGTGGCACACTTTGTTACGCCATTTAGCTTCGATAGCTTGGAAACGGTTCATGCTGCTCTGAATGGTCTTCTTCCTGCGGATATTAGAGTTCGGGAGATTAGTCCTGCTGTTCCCGACTTTCATGCGCGATTTTCTACAATAAGCAAGGTTTATCACTACAAGATATATAATGACACCATCTTGGATCCATTTCAGCGCTTCTACGCTTACCAATGCCTTTATAAACTCAATTCTGCAGTCATGAGAGAAGCTGCGAGTTATTTCATTGGAAAGCATGATTTTTCGGCTTTTGCGAATGCAGCGCAGAATGATCGAGTGGGAAATCCAGTGAAGGTCATTTTTCGGTTTGATGTCATAGAAATGGGACCTCTAATTCAGCTAGAAGTTGAGGGCTCTGGGTTCTTGTATAGACAAGTTCGGAATATGGTTGGCTTGTTGATTCAAATTGGAAAGGAAGCAATTTCTCCTGATGTTGTTCCTCAGATTTTGGCTTCTCGAGATCGCAAGGAGCTCGCCAAGTACGCTCCCTTCATTCCCCCTCACGGGCTCTGTCTTGTGAGCATTAAATATAACGAGGACCATCTAAAGCTTCCATCTGGTAGCCCTGGGACTAGTTCTGGTAGGTATCATACTGTGAGCAAATGTAAACTACCTTTTTATTGA

Protein sequence

MLQSLIPLALNGGCSLLMMVLHMQSPPTIQCLIEKALTQATKVERKDLRLVGASRTDTGVHAWGQVAHFVTPFSFDSLETVHAALNGLLPADIRVREISPAVPDFHARFSTISKVYHYKIYNDTILDPFQRFYAYQCLYKLNSAVMREAASYFIGKHDFSAFANAAQNDRVGNPVKVIFRFDVIEMGPLIQLEVEGSGFLYRQVRNMVGLLIQIGKEAISPDVVPQILASRDRKELAKYAPFIPPHGLCLVSIKYNEDHLKLPSGSPGTSSGRYHTVSKCKLPFY

Homology

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BLAST of Sed0006346 vs. NCBI nr
Match: XP_008458280.1 (PREDICTED: tRNA pseudouridine synthase A 1 isoform X2 [Cucumis melo])

HSP 1 Score: 459.1 bits (1180), Expect = 2.7e-125
Identity = 226/284 (79.58%), Postives = 248/284 (87.32%), Query Frame = 0


Query: 3   QSLIPLALNGGCSLLMMVLHMQ-SPPTIQCLIEKALTQATKVERKDLRLVGASRTDTGVH 62
           QS +  A NGGCS LM VL MQ SPPTIQCLIE+AL  ATK+ERKDL LVGASRTD GVH
Sbjct: 21  QSPLSPASNGGCSSLMTVLDMQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVH 80

Query: 63  AWGQVAHFVTPFSFDSLETVHAALNGLLPADIRVREISPAVPDFHARFSTISKVYHYKIY 122
           AWGQVAHFVTPF++DSLE+VHAALNGLLP DIRVRE+SPA PDFHARFST+SKVYHYKIY
Sbjct: 81  AWGQVAHFVTPFNYDSLESVHAALNGLLPVDIRVREMSPAAPDFHARFSTLSKVYHYKIY 140

Query: 123 NDTILDPFQRFYAYQCLYKLNSAVMREAASYFIGKHDFSAFANAAQNDRVGNPVKVIFRF 182
           N TI+DPFQR Y Y C+YKLN A M+EAA+YF+GKHDFSAF NAA N+RV NPVKVIFRF
Sbjct: 141 NGTIMDPFQRCYTYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNNRVVNPVKVIFRF 200

Query: 183 DVIEMGPLIQLEVEGSGFLYRQVRNMVGLLIQIGKEAISPDVVPQILASRDRKELAKYAP 242
           DVIEMG L+QLEVEGSGFLYRQVRNMVGLL+QIGKEA+ P+VVP+ILASRDRKELAKYA 
Sbjct: 201 DVIEMGALVQLEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRKELAKYAS 260

Query: 243 FIPPHGLCLVSIKYNEDHLKLPSGSPGTSSGRYHTVSKCKLPFY 286
            +PP GLCLVSI Y EDHLKLPS  PGTS GR  +V KCK+PFY
Sbjct: 261 GVPPQGLCLVSINYKEDHLKLPSDCPGTSLGRSRSVGKCKVPFY 304

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BLAST of Sed0006346 vs. NCBI nr
Match: XP_023549334.1 (uncharacterized protein LOC111807720 isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 458.4 bits (1178), Expect = 4.6e-125
Identity = 219/264 (82.95%), Postives = 239/264 (90.53%), Query Frame = 0


Query: 22  HMQSPPTIQCLIEKALTQATKVERKDLRLVGASRTDTGVHAWGQVAHFVTPFSFDSLETV 81
           + +SPPTIQCLIE+ALT AT++ERKDL LVGASRTDTGVHAWGQVAHF+TPF++DSL+ +
Sbjct: 72  YQRSPPTIQCLIEEALTGATRLERKDLHLVGASRTDTGVHAWGQVAHFITPFNYDSLDRI 131

Query: 82  HAALNGLLPADIRVREISPAVPDFHARFSTISKVYHYKIYNDTILDPFQRFYAYQCLYKL 141
           HAALNGLLPADIRVREISPAVPDFHARFST+SKVYHYKIYND ILDPFQR Y Y C+YKL
Sbjct: 132 HAALNGLLPADIRVREISPAVPDFHARFSTLSKVYHYKIYNDAILDPFQRCYTYHCVYKL 191

Query: 142 NSAVMREAASYFIGKHDFSAFANAAQNDRVGNPVKVIFRFDVIEMGPLIQLEVEGSGFLY 201
           N A M+EAA+YF+GKHDFSAFANAA NDRV NPVKV+FRFDVIEMG LIQLEVEGSGFLY
Sbjct: 192 NYAAMKEAANYFVGKHDFSAFANAAHNDRVVNPVKVVFRFDVIEMGALIQLEVEGSGFLY 251

Query: 202 RQVRNMVGLLIQIGKEAISPDVVPQILASRDRKELAKYAPFIPPHGLCLVSIKYNEDHLK 261
           RQVRNMV LL+QIGKEAI PDVVP+ILA+RDRKELAKY   IPPHGLCLV I Y EDHLK
Sbjct: 252 RQVRNMVALLLQIGKEAIPPDVVPKILATRDRKELAKYTTCIPPHGLCLVRINYKEDHLK 311

Query: 262 LPSGSPGTSSGRYHTVSKCKLPFY 286
           LPS  PGTS GR H+VSKCKLPFY
Sbjct: 312 LPSDCPGTSFGRSHSVSKCKLPFY 335

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BLAST of Sed0006346 vs. NCBI nr
Match: XP_023549333.1 (uncharacterized protein LOC111807720 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 458.4 bits (1178), Expect = 4.6e-125
Identity = 219/264 (82.95%), Postives = 239/264 (90.53%), Query Frame = 0


Query: 22  HMQSPPTIQCLIEKALTQATKVERKDLRLVGASRTDTGVHAWGQVAHFVTPFSFDSLETV 81
           + +SPPTIQCLIE+ALT AT++ERKDL LVGASRTDTGVHAWGQVAHF+TPF++DSL+ +
Sbjct: 74  YQRSPPTIQCLIEEALTGATRLERKDLHLVGASRTDTGVHAWGQVAHFITPFNYDSLDRI 133

Query: 82  HAALNGLLPADIRVREISPAVPDFHARFSTISKVYHYKIYNDTILDPFQRFYAYQCLYKL 141
           HAALNGLLPADIRVREISPAVPDFHARFST+SKVYHYKIYND ILDPFQR Y Y C+YKL
Sbjct: 134 HAALNGLLPADIRVREISPAVPDFHARFSTLSKVYHYKIYNDAILDPFQRCYTYHCVYKL 193

Query: 142 NSAVMREAASYFIGKHDFSAFANAAQNDRVGNPVKVIFRFDVIEMGPLIQLEVEGSGFLY 201
           N A M+EAA+YF+GKHDFSAFANAA NDRV NPVKV+FRFDVIEMG LIQLEVEGSGFLY
Sbjct: 194 NYAAMKEAANYFVGKHDFSAFANAAHNDRVVNPVKVVFRFDVIEMGALIQLEVEGSGFLY 253

Query: 202 RQVRNMVGLLIQIGKEAISPDVVPQILASRDRKELAKYAPFIPPHGLCLVSIKYNEDHLK 261
           RQVRNMV LL+QIGKEAI PDVVP+ILA+RDRKELAKY   IPPHGLCLV I Y EDHLK
Sbjct: 254 RQVRNMVALLLQIGKEAIPPDVVPKILATRDRKELAKYTTCIPPHGLCLVRINYKEDHLK 313

Query: 262 LPSGSPGTSSGRYHTVSKCKLPFY 286
           LPS  PGTS GR H+VSKCKLPFY
Sbjct: 314 LPSDCPGTSFGRSHSVSKCKLPFY 337

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BLAST of Sed0006346 vs. NCBI nr
Match: XP_023549335.1 (uncharacterized protein LOC111807720 isoform X3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 458.4 bits (1178), Expect = 4.6e-125
Identity = 219/264 (82.95%), Postives = 239/264 (90.53%), Query Frame = 0


Query: 22  HMQSPPTIQCLIEKALTQATKVERKDLRLVGASRTDTGVHAWGQVAHFVTPFSFDSLETV 81
           + +SPPTIQCLIE+ALT AT++ERKDL LVGASRTDTGVHAWGQVAHF+TPF++DSL+ +
Sbjct: 72  YQRSPPTIQCLIEEALTGATRLERKDLHLVGASRTDTGVHAWGQVAHFITPFNYDSLDRI 131

Query: 82  HAALNGLLPADIRVREISPAVPDFHARFSTISKVYHYKIYNDTILDPFQRFYAYQCLYKL 141
           HAALNGLLPADIRVREISPAVPDFHARFST+SKVYHYKIYND ILDPFQR Y Y C+YKL
Sbjct: 132 HAALNGLLPADIRVREISPAVPDFHARFSTLSKVYHYKIYNDAILDPFQRCYTYHCVYKL 191

Query: 142 NSAVMREAASYFIGKHDFSAFANAAQNDRVGNPVKVIFRFDVIEMGPLIQLEVEGSGFLY 201
           N A M+EAA+YF+GKHDFSAFANAA NDRV NPVKV+FRFDVIEMG LIQLEVEGSGFLY
Sbjct: 192 NYAAMKEAANYFVGKHDFSAFANAAHNDRVVNPVKVVFRFDVIEMGALIQLEVEGSGFLY 251

Query: 202 RQVRNMVGLLIQIGKEAISPDVVPQILASRDRKELAKYAPFIPPHGLCLVSIKYNEDHLK 261
           RQVRNMV LL+QIGKEAI PDVVP+ILA+RDRKELAKY   IPPHGLCLV I Y EDHLK
Sbjct: 252 RQVRNMVALLLQIGKEAIPPDVVPKILATRDRKELAKYTTCIPPHGLCLVRINYKEDHLK 311

Query: 262 LPSGSPGTSSGRYHTVSKCKLPFY 286
           LPS  PGTS GR H+VSKCKLPFY
Sbjct: 312 LPSDCPGTSFGRSHSVSKCKLPFY 335

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BLAST of Sed0006346 vs. NCBI nr
Match: XP_023549337.1 (uncharacterized protein LOC111807720 isoform X4 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 458.4 bits (1178), Expect = 4.6e-125
Identity = 219/264 (82.95%), Postives = 239/264 (90.53%), Query Frame = 0


Query: 22  HMQSPPTIQCLIEKALTQATKVERKDLRLVGASRTDTGVHAWGQVAHFVTPFSFDSLETV 81
           + +SPPTIQCLIE+ALT AT++ERKDL LVGASRTDTGVHAWGQVAHF+TPF++DSL+ +
Sbjct: 70  YQRSPPTIQCLIEEALTGATRLERKDLHLVGASRTDTGVHAWGQVAHFITPFNYDSLDRI 129

Query: 82  HAALNGLLPADIRVREISPAVPDFHARFSTISKVYHYKIYNDTILDPFQRFYAYQCLYKL 141
           HAALNGLLPADIRVREISPAVPDFHARFST+SKVYHYKIYND ILDPFQR Y Y C+YKL
Sbjct: 130 HAALNGLLPADIRVREISPAVPDFHARFSTLSKVYHYKIYNDAILDPFQRCYTYHCVYKL 189

Query: 142 NSAVMREAASYFIGKHDFSAFANAAQNDRVGNPVKVIFRFDVIEMGPLIQLEVEGSGFLY 201
           N A M+EAA+YF+GKHDFSAFANAA NDRV NPVKV+FRFDVIEMG LIQLEVEGSGFLY
Sbjct: 190 NYAAMKEAANYFVGKHDFSAFANAAHNDRVVNPVKVVFRFDVIEMGALIQLEVEGSGFLY 249

Query: 202 RQVRNMVGLLIQIGKEAISPDVVPQILASRDRKELAKYAPFIPPHGLCLVSIKYNEDHLK 261
           RQVRNMV LL+QIGKEAI PDVVP+ILA+RDRKELAKY   IPPHGLCLV I Y EDHLK
Sbjct: 250 RQVRNMVALLLQIGKEAIPPDVVPKILATRDRKELAKYTTCIPPHGLCLVRINYKEDHLK 309

Query: 262 LPSGSPGTSSGRYHTVSKCKLPFY 286
           LPS  PGTS GR H+VSKCKLPFY
Sbjct: 310 LPSDCPGTSFGRSHSVSKCKLPFY 333

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BLAST of Sed0006346 vs. ExPASy Swiss-Prot
Match: B1I1B5 (tRNA pseudouridine synthase A OS=Desulforudis audaxviator (strain MP104C) OX=477974 GN=truA PE=3 SV=1)

HSP 1 Score: 180.3 bits (456), Expect = 3.2e-44
Identity = 96/230 (41.74%), Postives = 135/230 (58.70%), Query Frame = 0


Query: 27  PTIQCLIEKALTQATKVERKDLRLVGASRTDTGVHAWGQVAHFVTPFSFDSLETVHAALN 86
           PTIQ  +E+ L + +      L++ GASRTD GVHA GQV +FVT       E + AALN
Sbjct: 27  PTIQETLERCLAELSGAA---LKVTGASRTDAGVHARGQVVNFVTGRWGIPTERIPAALN 86

Query: 87  GLLPADIRVREISPAVPDFHARFSTISKVYHYKIYNDTILDPFQRFYAYQCLYKLNSAVM 146
           G+LP DI   +      DFHAR+S ++K Y Y +YN  +  PFQR Y+      L+   M
Sbjct: 87  GVLPGDIAALDAREVPADFHARYSAVAKTYSYTLYNHPVRSPFQRLYSLHVPRALDLRAM 146

Query: 147 REAASYFIGKHDFSAFANAAQNDRVGNPVKVIFRFDVIEMGPLIQLEVEGSGFLYRQVRN 206
           R AA + +G+HDFS F  A +   V + V+ + R D+    PL++L     GFLY  VR 
Sbjct: 147 RLAARHLMGRHDFSTFQAAGR--PVQSAVRTLTRADIEAQAPLVRLVFRADGFLYNMVRI 206

Query: 207 MVGLLIQIGKEAISPDVVPQILASRDRKELAKYAPFIPPHGLCLVSIKYN 257
           M G L+++G E + P+ +P I+AS DR   A+  P +PPHGLCL  ++Y+
Sbjct: 207 MAGTLLEVGLERLDPEALPDIIASGDR---ARAGPTVPPHGLCLEEVEYD 248

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BLAST of Sed0006346 vs. ExPASy Swiss-Prot
Match: Q6MEE7 (tRNA pseudouridine synthase A 1 OS=Protochlamydia amoebophila (strain UWE25) OX=264201 GN=truA1 PE=3 SV=1)

HSP 1 Score: 176.8 bits (447), Expect = 3.5e-43
Identity = 93/233 (39.91%), Postives = 145/233 (62.23%), Query Frame = 0


Query: 28  TIQCLIEKALTQATKVERKDLRLVGASRTDTGVHAWGQVAHFVTPFSFDSLETVHAALNG 87
           +IQ  I++AL    K E+  + LVG+ RTD GVHA GQVAHF      D L  +H +LNG
Sbjct: 26  SIQQKIQEALCILLKKEK--VVLVGSGRTDAGVHAKGQVAHFHFQDYID-LSRLHVSLNG 85

Query: 88  LLPADIRVREISPAVPDFHARFSTISKVYHYKIYNDTILDPFQRFYAYQCLYKLNSAVMR 147
           LLP DIR++ + P  P FH+++S I K YHY ++ + ++DPFQR Y++    K++  +++
Sbjct: 86  LLPRDIRIKAVEPVSPRFHSQYSAIRKEYHYYLHLNKVMDPFQRLYSWHFQRKIDVNILK 145

Query: 148 EAASYFIGKHDFSAFANAAQNDRVG-NPVKVIFRFDVIEMGPLIQLEVEGSGFLYRQVRN 207
           +AA  F G HDF++FAN A       NPV+ ++R D+      ++LE EG GFLY+ VRN
Sbjct: 146 KAAILFTGTHDFTSFANEAHRGTAAKNPVRTLYRLDIKPNEGGLRLEFEGDGFLYKMVRN 205

Query: 208 MVGLLIQIGKEAISPDVVPQILASRDRKELAKYAPFIPPHGLCLVSIKYNEDH 260
           +VG L+ +     + + + QI A+++R++ +  A   PP GL L+ + Y  ++
Sbjct: 206 IVGTLMDVASHKRAIEEINQIFAAKNRRQASLAA---PPEGLFLIQVFYENEN 252

[Close]

BLAST of Sed0006346 vs. ExPASy Swiss-Prot
Match: A4J151 (tRNA pseudouridine synthase A OS=Desulfotomaculum reducens (strain MI-1) OX=349161 GN=truA PE=3 SV=1)

HSP 1 Score: 166.0 bits (419), Expect = 6.2e-40
Identity = 91/228 (39.91%), Postives = 135/228 (59.21%), Query Frame = 0


Query: 28  TIQCLIEKALTQATKVERKDLRLVGASRTDTGVHAWGQVAHFVTPFSFDSLETVHAALNG 87
           TIQ  +EKAL   + + +  ++++GA RTD GVHA GQV +F +       E    ALN 
Sbjct: 28  TIQEALEKAL---STIAQTPIQVIGAGRTDAGVHAQGQVVNFRSEKWPVPAEKAPLALNV 87

Query: 88  LLPADIRVREISPAVPDFHARFSTISKVYHYKIYNDTILDPFQRFYAYQCLYKLNSAVMR 147
           LLP DI+V +      DFHARFS ++K Y Y IY+  ++ PF R+Y Y    +L+   M+
Sbjct: 88  LLPGDIKVVKAEEVPMDFHARFSAVAKTYRYSIYHHRVMSPFHRYYCYHEPRRLDVNAMQ 147

Query: 148 EAASYFIGKHDFSAFANAAQNDRVGNPVKVIFRFDVIEMGPLIQLEVEGSGFLYRQVRNM 207
           E A+Y +G +DF +F   AQ   V + ++ I+R D+IE  P+I L + G+GFLY  VR +
Sbjct: 148 EGAAYLLGTYDFKSF--QAQGTPVKDTIRTIYRADIIEDAPVINLYLRGNGFLYNMVRII 207

Query: 208 VGLLIQIGKEAISPDVVPQILASRDRKELAKYAPFIPPHGLCLVSIKY 256
            G L+ IG   I P+ + +I+ S++R      A   PP GLCL+ ++Y
Sbjct: 208 TGTLLNIGFGKIKPEDMVKIIESKNRTLAGTTA---PPQGLCLMEVEY 247

[Close]

BLAST of Sed0006346 vs. ExPASy Swiss-Prot
Match: P70973 (tRNA pseudouridine synthase A OS=Bacillus subtilis (strain 168) OX=224308 GN=truA PE=3 SV=2)

HSP 1 Score: 163.3 bits (412), Expect = 4.0e-39
Identity = 97/229 (42.36%), Postives = 131/229 (57.21%), Query Frame = 0


Query: 28  TIQCLIEKALTQATKVERKDLRLVGASRTDTGVHAWGQVAHFVTPFSFDSLETVHAALNG 87
           T+Q  +EKAL    K  +  + +V + RTD+GVHA GQV HF TP S  + E    ALN 
Sbjct: 25  TVQDELEKALAVLHK-SKDRIPVVSSGRTDSGVHAAGQVIHFDTPLSIPA-ERWPYALNA 84

Query: 88  LLPADIRVREISPAVPDFHARFSTISKVYHYKIYNDTILDPFQRFYAYQCLYKLNSAVMR 147
           LLP DI V++   A   FHARFS + K Y Y +Y +   D F+R YAY   Y+LN   MR
Sbjct: 85  LLPDDIAVKQAEIADDGFHARFSAVKKEYRYFVYTEKHPDVFKRHYAYHFSYRLNVQDMR 144

Query: 148 EAASYFIGKHDFSAFANAAQNDRVGNPVKVIFRFDVIEMGPLIQLEVEGSGFLYRQVRNM 207
           EAA + IG HDF++F   A    V + V+ I+  D  E    +Q+ + GSGFLY  VR +
Sbjct: 145 EAAKHLIGTHDFTSF--CAAKTEVQDKVRTIYELDWTETADGLQMRITGSGFLYNMVRII 204

Query: 208 VGLLIQIGKEAISPDVVPQILASRDRKELAKYAPFIPPHGLCLVSIKYN 257
            G L+  G   ISPD V  +L ++DR+   + A   P HGL L ++ Y+
Sbjct: 205 AGTLLDAGIGKISPDEVKSMLEAKDREAAGRTA---PGHGLYLWNVYYD 246

[Close]

BLAST of Sed0006346 vs. ExPASy Swiss-Prot
Match: A8F9B7 (tRNA pseudouridine synthase A OS=Bacillus pumilus (strain SAFR-032) OX=315750 GN=truA PE=3 SV=1)

HSP 1 Score: 158.7 bits (400), Expect = 9.9e-38
Identity = 94/229 (41.05%), Postives = 132/229 (57.64%), Query Frame = 0


Query: 28  TIQCLIEKALTQATKVERKDLRLVGASRTDTGVHAWGQVAHFVTPFSFDSLETVHAALNG 87
           T+Q  IE AL +  K +   + +V + RTD+GVHA GQV HF TP S   +E    ALN 
Sbjct: 25  TVQTEIESALAKMHKQDEL-VPIVASGRTDSGVHAKGQVIHFDTPLSI-PMERWPFALNS 84

Query: 88  LLPADIRVREISPAVPDFHARFSTISKVYHYKIYNDTILDPFQRFYAYQCLYKLNSAVMR 147
           LLP DIRV +       FHARFS +SK Y YK+  +T  + F R YA    Y+L++  MR
Sbjct: 85  LLPDDIRVLKAEEVDESFHARFSVVSKEYRYKVSTETHQNVFTRQYACHFPYRLDADKMR 144

Query: 148 EAASYFIGKHDFSAFANAAQNDRVGNPVKVIFRFDVIEMGPLIQLEVEGSGFLYRQVRNM 207
           EAA Y IG HDF++F   A N  V + V+ I+  +   +   +++ V G+GFLY  VR +
Sbjct: 145 EAAGYLIGTHDFTSF--CAANTEVQDKVREIYTLEWKNVSDGLEMRVRGNGFLYNMVRII 204

Query: 208 VGLLIQIGKEAISPDVVPQILASRDRKELAKYAPFIPPHGLCLVSIKYN 257
            G L+++G     PD +  +LA+R+R+   K A   P HGL L  + Y+
Sbjct: 205 AGTLLEVGSGKFHPDEIKAMLAARNREAAGKTA---PSHGLYLWEVFYD 246

[Close]

BLAST of Sed0006346 vs. ExPASy TrEMBL
Match: A0A1S3C7H4 (tRNA pseudouridine synthase OS=Cucumis melo OX=3656 GN=LOC103497746 PE=3 SV=1)

HSP 1 Score: 459.1 bits (1180), Expect = 1.3e-125
Identity = 226/284 (79.58%), Postives = 248/284 (87.32%), Query Frame = 0


Query: 3   QSLIPLALNGGCSLLMMVLHMQ-SPPTIQCLIEKALTQATKVERKDLRLVGASRTDTGVH 62
           QS +  A NGGCS LM VL MQ SPPTIQCLIE+AL  ATK+ERKDL LVGASRTD GVH
Sbjct: 21  QSPLSPASNGGCSSLMTVLDMQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVH 80

Query: 63  AWGQVAHFVTPFSFDSLETVHAALNGLLPADIRVREISPAVPDFHARFSTISKVYHYKIY 122
           AWGQVAHFVTPF++DSLE+VHAALNGLLP DIRVRE+SPA PDFHARFST+SKVYHYKIY
Sbjct: 81  AWGQVAHFVTPFNYDSLESVHAALNGLLPVDIRVREMSPAAPDFHARFSTLSKVYHYKIY 140

Query: 123 NDTILDPFQRFYAYQCLYKLNSAVMREAASYFIGKHDFSAFANAAQNDRVGNPVKVIFRF 182
           N TI+DPFQR Y Y C+YKLN A M+EAA+YF+GKHDFSAF NAA N+RV NPVKVIFRF
Sbjct: 141 NGTIMDPFQRCYTYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNNRVVNPVKVIFRF 200

Query: 183 DVIEMGPLIQLEVEGSGFLYRQVRNMVGLLIQIGKEAISPDVVPQILASRDRKELAKYAP 242
           DVIEMG L+QLEVEGSGFLYRQVRNMVGLL+QIGKEA+ P+VVP+ILASRDRKELAKYA 
Sbjct: 201 DVIEMGALVQLEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRKELAKYAS 260

Query: 243 FIPPHGLCLVSIKYNEDHLKLPSGSPGTSSGRYHTVSKCKLPFY 286
            +PP GLCLVSI Y EDHLKLPS  PGTS GR  +V KCK+PFY
Sbjct: 261 GVPPQGLCLVSINYKEDHLKLPSDCPGTSLGRSRSVGKCKVPFY 304

[Close]

BLAST of Sed0006346 vs. ExPASy TrEMBL
Match: A0A6J1C9Y0 (tRNA pseudouridine synthase OS=Momordica charantia OX=3673 GN=LOC111009557 PE=3 SV=1)

HSP 1 Score: 453.4 bits (1165), Expect = 7.2e-124
Identity = 219/264 (82.95%), Postives = 235/264 (89.02%), Query Frame = 0


Query: 22  HMQSPPTIQCLIEKALTQATKVERKDLRLVGASRTDTGVHAWGQVAHFVTPFSFDSLETV 81
           + +SPPTIQCL+EKALT+ATK+ERKDL LVGASRTD GVHAWGQVAHF+TPF++ SLE V
Sbjct: 78  YQESPPTIQCLVEKALTRATKLERKDLHLVGASRTDRGVHAWGQVAHFITPFNYHSLENV 137

Query: 82  HAALNGLLPADIRVREISPAVPDFHARFSTISKVYHYKIYNDTILDPFQRFYAYQCLYKL 141
           HAALNGLLPADIRVREISPA+P FHARFSTISKVYHYKIYNDT LDPF R Y Y C YKL
Sbjct: 138 HAALNGLLPADIRVREISPAMPHFHARFSTISKVYHYKIYNDTFLDPFHRCYTYHCAYKL 197

Query: 142 NSAVMREAASYFIGKHDFSAFANAAQNDRVGNPVKVIFRFDVIEMGPLIQLEVEGSGFLY 201
           N AVMREAA+YFIGKHDFSAFANAA NDRVGNPVK+IFRFDVIEMG L+QLEVEGSGFLY
Sbjct: 198 NYAVMREAANYFIGKHDFSAFANAAHNDRVGNPVKIIFRFDVIEMGALVQLEVEGSGFLY 257

Query: 202 RQVRNMVGLLIQIGKEAISPDVVPQILASRDRKELAKYAPFIPPHGLCLVSIKYNEDHLK 261
           RQVRNMV LL+QIGKEAI PDVVP+ILASRDRKELAKYA   PP GLCL++I YNEDHLK
Sbjct: 258 RQVRNMVALLLQIGKEAIPPDVVPKILASRDRKELAKYASGAPPQGLCLMTINYNEDHLK 317

Query: 262 LPSGSPGTSSGRYHTVSKCKLPFY 286
            PS  P TS GR H VSKCKLPFY
Sbjct: 318 FPSDCPETSFGRRHHVSKCKLPFY 341

[Close]

BLAST of Sed0006346 vs. ExPASy TrEMBL
Match: A0A6J1H621 (tRNA pseudouridine synthase OS=Cucurbita moschata OX=3662 GN=LOC111460439 PE=3 SV=1)

HSP 1 Score: 452.2 bits (1162), Expect = 1.6e-123
Identity = 215/264 (81.44%), Postives = 237/264 (89.77%), Query Frame = 0


Query: 22  HMQSPPTIQCLIEKALTQATKVERKDLRLVGASRTDTGVHAWGQVAHFVTPFSFDSLETV 81
           + +SPPTIQCL+E+ALT AT++ERKDL LVGASRTDTGVHAWGQVAHF+TPF++DSL+ +
Sbjct: 71  YQRSPPTIQCLVEEALTGATRLERKDLHLVGASRTDTGVHAWGQVAHFITPFNYDSLDRI 130

Query: 82  HAALNGLLPADIRVREISPAVPDFHARFSTISKVYHYKIYNDTILDPFQRFYAYQCLYKL 141
           HAALNGLLPADIRVRE+SPAVPDFHARFST+SKVYHYKIYND ILDPFQR Y Y C+YKL
Sbjct: 131 HAALNGLLPADIRVREMSPAVPDFHARFSTLSKVYHYKIYNDAILDPFQRCYTYHCVYKL 190

Query: 142 NSAVMREAASYFIGKHDFSAFANAAQNDRVGNPVKVIFRFDVIEMGPLIQLEVEGSGFLY 201
           N A M+EAA+YF+GKHDFSAFAN A NDRV NPVKVIFRFDVIEMG LIQLEVEGSGFLY
Sbjct: 191 NYAAMKEAANYFVGKHDFSAFANTAHNDRVVNPVKVIFRFDVIEMGALIQLEVEGSGFLY 250

Query: 202 RQVRNMVGLLIQIGKEAISPDVVPQILASRDRKELAKYAPFIPPHGLCLVSIKYNEDHLK 261
           RQVRNMV LL+QIGKEAI PD+VP+ILA+RDRKELAKY   IPP GLCLV I Y EDHLK
Sbjct: 251 RQVRNMVALLLQIGKEAIPPDIVPKILATRDRKELAKYTTCIPPQGLCLVRINYKEDHLK 310

Query: 262 LPSGSPGTSSGRYHTVSKCKLPFY 286
           LPS  PGTS GR H+VSKCKLPFY
Sbjct: 311 LPSDCPGTSFGRSHSVSKCKLPFY 334

[Close]

BLAST of Sed0006346 vs. ExPASy TrEMBL
Match: A0A6J1H4M7 (tRNA pseudouridine synthase OS=Cucurbita moschata OX=3662 GN=LOC111460439 PE=3 SV=1)

HSP 1 Score: 452.2 bits (1162), Expect = 1.6e-123
Identity = 215/264 (81.44%), Postives = 237/264 (89.77%), Query Frame = 0


Query: 22  HMQSPPTIQCLIEKALTQATKVERKDLRLVGASRTDTGVHAWGQVAHFVTPFSFDSLETV 81
           + +SPPTIQCL+E+ALT AT++ERKDL LVGASRTDTGVHAWGQVAHF+TPF++DSL+ +
Sbjct: 73  YQRSPPTIQCLVEEALTGATRLERKDLHLVGASRTDTGVHAWGQVAHFITPFNYDSLDRI 132

Query: 82  HAALNGLLPADIRVREISPAVPDFHARFSTISKVYHYKIYNDTILDPFQRFYAYQCLYKL 141
           HAALNGLLPADIRVRE+SPAVPDFHARFST+SKVYHYKIYND ILDPFQR Y Y C+YKL
Sbjct: 133 HAALNGLLPADIRVREMSPAVPDFHARFSTLSKVYHYKIYNDAILDPFQRCYTYHCVYKL 192

Query: 142 NSAVMREAASYFIGKHDFSAFANAAQNDRVGNPVKVIFRFDVIEMGPLIQLEVEGSGFLY 201
           N A M+EAA+YF+GKHDFSAFAN A NDRV NPVKVIFRFDVIEMG LIQLEVEGSGFLY
Sbjct: 193 NYAAMKEAANYFVGKHDFSAFANTAHNDRVVNPVKVIFRFDVIEMGALIQLEVEGSGFLY 252

Query: 202 RQVRNMVGLLIQIGKEAISPDVVPQILASRDRKELAKYAPFIPPHGLCLVSIKYNEDHLK 261
           RQVRNMV LL+QIGKEAI PD+VP+ILA+RDRKELAKY   IPP GLCLV I Y EDHLK
Sbjct: 253 RQVRNMVALLLQIGKEAIPPDIVPKILATRDRKELAKYTTCIPPQGLCLVRINYKEDHLK 312

Query: 262 LPSGSPGTSSGRYHTVSKCKLPFY 286
           LPS  PGTS GR H+VSKCKLPFY
Sbjct: 313 LPSDCPGTSFGRSHSVSKCKLPFY 336

[Close]

BLAST of Sed0006346 vs. ExPASy TrEMBL
Match: A0A6J1L0L1 (tRNA pseudouridine synthase OS=Cucurbita maxima OX=3661 GN=LOC111499282 PE=3 SV=1)

HSP 1 Score: 450.3 bits (1157), Expect = 6.1e-123
Identity = 217/264 (82.20%), Postives = 235/264 (89.02%), Query Frame = 0


Query: 22  HMQSPPTIQCLIEKALTQATKVERKDLRLVGASRTDTGVHAWGQVAHFVTPFSFDSLETV 81
           + +SPPTIQCLIE+ALT AT++ERKDL LVGASRTD GVHAWGQVAHF+TPF++DSL+ +
Sbjct: 70  YQRSPPTIQCLIEEALTGATRLERKDLHLVGASRTDKGVHAWGQVAHFITPFNYDSLDRI 129

Query: 82  HAALNGLLPADIRVREISPAVPDFHARFSTISKVYHYKIYNDTILDPFQRFYAYQCLYKL 141
           HAALNGLLPADIRVREISPAVPDFHARFST+SKVYHYKIYND ILDPFQR Y Y C+YKL
Sbjct: 130 HAALNGLLPADIRVREISPAVPDFHARFSTLSKVYHYKIYNDAILDPFQRCYTYHCVYKL 189

Query: 142 NSAVMREAASYFIGKHDFSAFANAAQNDRVGNPVKVIFRFDVIEMGPLIQLEVEGSGFLY 201
           N A M+EAA+YF+GKHDFSAFAN A NDRV NPVKVIFRFDVIEMG LIQLEVEGSGFLY
Sbjct: 190 NYAAMKEAANYFVGKHDFSAFANTAHNDRVVNPVKVIFRFDVIEMGALIQLEVEGSGFLY 249

Query: 202 RQVRNMVGLLIQIGKEAISPDVVPQILASRDRKELAKYAPFIPPHGLCLVSIKYNEDHLK 261
           RQVRNMV LL+QIGKEAI PDVVP+ILASRDRK LAKY   IPP GLCLV I Y EDHLK
Sbjct: 250 RQVRNMVALLLQIGKEAIPPDVVPKILASRDRKVLAKYTTCIPPQGLCLVRINYKEDHLK 309

Query: 262 LPSGSPGTSSGRYHTVSKCKLPFY 286
           LPS  PGTS GR H+VSKCKLPFY
Sbjct: 310 LPSDCPGTSFGRSHSVSKCKLPFY 333

[Close]

BLAST of Sed0006346 vs. TAIR 10
Match: AT3G06950.1 (Pseudouridine synthase family protein )

HSP 1 Score: 390.2 bits (1001), Expect = 1.4e-108
Identity = 185/265 (69.81%), Postives = 221/265 (83.40%), Query Frame = 0


Query: 22  HMQSPPTIQCLIEKALTQATKVERKDLRLVGASRTDTGVHAWGQVAHFVTPFSFDSLETV 81
           + +SPPTIQ ++EKAL Q T++ RK+L+L+GA RTD GVHAWGQVAHFVTPF++ SL++ 
Sbjct: 59  YQESPPTIQSMLEKALIQITELGRKELQLIGAGRTDAGVHAWGQVAHFVTPFNYTSLDSF 118

Query: 82  HAALNGLLPADIRVREISPAVPDFHARFSTISKVYHYKIYNDTILDPFQRFYAYQCLYKL 141
           HAALNGLLP DIRVRE+S AVP+FHARFS  SKVY Y+IYNDT +DPFQR +AY C YKL
Sbjct: 119 HAALNGLLPKDIRVRELSAAVPEFHARFSASSKVYRYQIYNDTFMDPFQRHWAYHCAYKL 178

Query: 142 NSAVMREAASYFIGKHDFSAFANAAQNDRVGNPVKVIFRFDVIEMGPLIQLEVEGSGFLY 201
           N++ MREAA+ F+GKHDFSAFANA + D V +P+K I RFDVI+MG L+QLEVEGSGFLY
Sbjct: 179 NASKMREAANLFVGKHDFSAFANATREDGVPDPLKTISRFDVIQMGSLLQLEVEGSGFLY 238

Query: 202 RQVRNMVGLLIQIGKEAISPDVVPQILASRDRKELAKYAPF-IPPHGLCLVSIKYNEDHL 261
           RQVRNMV LLIQIGKEA+  D+VP IL ++DR+ LAKY      PHGLCLVS+KY EDHL
Sbjct: 239 RQVRNMVALLIQIGKEALDSDIVPMILETKDRRVLAKYTSLPASPHGLCLVSVKYKEDHL 298

Query: 262 KLPSGSPGTSSGRYHTVSKCKLPFY 286
           KLP   P TSSGR+HT++KCKLPFY
Sbjct: 299 KLPLDCPVTSSGRHHTITKCKLPFY 323

[Close]

BLAST of Sed0006346 vs. TAIR 10
Match: AT1G34150.1 (Pseudouridine synthase family protein )

HSP 1 Score: 75.1 bits (183), Expect = 1.0e-13
Identity = 74/245 (30.20%), Postives = 100/245 (40.82%), Query Frame = 0


Query: 24  QSPPTIQCLIEKAL--TQATKVERKDLRLVGASRTDTGVHAWGQV------AHFVTPFSF 83
           Q  P+I+  + KAL  T+    ++KD       RTD GV + GQV      +   +P   
Sbjct: 111 QMEPSIESEVFKALERTRLLVGDKKDSCYSRCGRTDKGVSSTGQVIALFLRSRLKSP-PG 170

Query: 84  DSLETVHA------------ALNGLLPADIRVREISPAVPDFHARFSTISKVYHYKIYND 143
           DS   V+              LN  LP DIRV   SPA  DFHARFS  ++ Y Y  +  
Sbjct: 171 DSKAQVNGRTGERPEYDYVRVLNRALPDDIRVIGWSPAPIDFHARFSCYAREYKYFFWRQ 230

Query: 144 TILDPFQRFYAYQCLYKLNSAVMREAASYFIGKHDFSAFANAAQNDRVGNPVKVIFRFDV 203
                            LN + M  A   FIG+HDF  F        V N      R   
Sbjct: 231 ----------------NLNLSAMDFAGKKFIGEHDFRNFCKM----DVANVHCYTRRVTF 290

Query: 204 IEMGP---------LIQLEVEGSGFLYRQVRNMVGLLIQIGKEAISPDVVPQILASRDRK 240
            E+ P         L    + GS FL+ Q+R MV +L  IG+   S DV+  +L ++   
Sbjct: 291 FEVSPCQNSHEGDQLCTFTMRGSAFLWHQIRCMVAVLFMIGQGVESVDVIDTLLDTKKTP 334

The following BLAST results are available for this feature:

BLAST of Sed0006346 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008458280.1	2.7e-125	79.58	PREDICTED: tRNA pseudouridine synthase A 1 isoform X2 [Cucumis melo]	[more]
XP_023549334.1	4.6e-125	82.95	uncharacterized protein LOC111807720 isoform X2 [Cucurbita pepo subsp. pepo]	[more]
XP_023549333.1	4.6e-125	82.95	uncharacterized protein LOC111807720 isoform X1 [Cucurbita pepo subsp. pepo]	[more]
XP_023549335.1	4.6e-125	82.95	uncharacterized protein LOC111807720 isoform X3 [Cucurbita pepo subsp. pepo]	[more]
XP_023549337.1	4.6e-125	82.95	uncharacterized protein LOC111807720 isoform X4 [Cucurbita pepo subsp. pepo]	[more]

BLAST of Sed0006346 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
B1I1B5	3.2e-44	41.74	tRNA pseudouridine synthase A OS=Desulforudis audaxviator (strain MP104C) OX=477...	[more]
Q6MEE7	3.5e-43	39.91	tRNA pseudouridine synthase A 1 OS=Protochlamydia amoebophila (strain UWE25) OX=...	[more]
A4J151	6.2e-40	39.91	tRNA pseudouridine synthase A OS=Desulfotomaculum reducens (strain MI-1) OX=3491...	[more]
P70973	4.0e-39	42.36	tRNA pseudouridine synthase A OS=Bacillus subtilis (strain 168) OX=224308 GN=tru...	[more]
A8F9B7	9.9e-38	41.05	tRNA pseudouridine synthase A OS=Bacillus pumilus (strain SAFR-032) OX=315750 GN...	[more]

BLAST of Sed0006346 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3C7H4	1.3e-125	79.58	tRNA pseudouridine synthase OS=Cucumis melo OX=3656 GN=LOC103497746 PE=3 SV=1	[more]
A0A6J1C9Y0	7.2e-124	82.95	tRNA pseudouridine synthase OS=Momordica charantia OX=3673 GN=LOC111009557 PE=3 ...	[more]
A0A6J1H621	1.6e-123	81.44	tRNA pseudouridine synthase OS=Cucurbita moschata OX=3662 GN=LOC111460439 PE=3 S...	[more]
A0A6J1H4M7	1.6e-123	81.44	tRNA pseudouridine synthase OS=Cucurbita moschata OX=3662 GN=LOC111460439 PE=3 S...	[more]
A0A6J1L0L1	6.1e-123	82.20	tRNA pseudouridine synthase OS=Cucurbita maxima OX=3661 GN=LOC111499282 PE=3 SV=...	[more]

BLAST of Sed0006346 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G06950.1	1.4e-108	69.81	Pseudouridine synthase family protein	[more]
AT1G34150.1	1.0e-13	30.20	Pseudouridine synthase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Chayote (edule) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001406	Pseudouridine synthase I, TruA	PIRSF	PIRSF001430	PSU-A	coord: 10..213 e-value: 1.1E-53 score: 180.2
IPR001406	Pseudouridine synthase I, TruA	PANTHER	PTHR11142	PSEUDOURIDYLATE SYNTHASE	coord: 12..212
IPR001406	Pseudouridine synthase I, TruA	CDD	cd02570	PseudoU_synth_EcTruA	coord: 12..202 e-value: 1.19849E-79 score: 236.986
IPR020094	Pseudouridine synthase TruA/RsuA/RluB/E/F, N-terminal	GENE3D	3.30.70.580		coord: 9..58 e-value: 2.1E-5 score: 26.7
IPR020095	Pseudouridine synthase I, TruA, C-terminal	GENE3D	3.30.70.660		coord: 59..200 e-value: 9.6E-39 score: 134.3
IPR020097	Pseudouridine synthase I, TruA, alpha/beta domain	PFAM	PF01416	PseudoU_synth_1	coord: 96..203 e-value: 5.7E-27 score: 94.3
None	No IPR available	PANTHER	PTHR11142:SF0	TRNA PSEUDOURIDINE SYNTHASE-LIKE 1	coord: 12..212
IPR020103	Pseudouridine synthase, catalytic domain superfamily	SUPERFAMILY	55120	Pseudouridine synthase	coord: 11..207

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Sed0006346.1	Sed0006346.1	mRNA
Sed0006346.2	Sed0006346.2	mRNA
Sed0006346.3	Sed0006346.3	mRNA
Sed0006346.4	Sed0006346.4	mRNA
Sed0006346.5	Sed0006346.5	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0034196	acylglycerol transport
biological_process	GO:1990052	ER to chloroplast lipid transport
biological_process	GO:0001522	pseudouridine synthesis
biological_process	GO:0008033	tRNA processing
biological_process	GO:0009451	RNA modification
molecular_function	GO:0070300	phosphatidic acid binding
molecular_function	GO:0003723	RNA binding
molecular_function	GO:0106029	tRNA pseudouridine synthase activity
molecular_function	GO:0009982	pseudouridine synthase activity