Sed0005150 (gene) Chayote v1

Overview


Name	Sed0005150
Type	gene
Organism	Sechium edule (Chayote v1)
Description	tRNA pseudouridine synthase
Location	LG12: 31967732 .. 31973909 (+)
RNA-Seq Expression	Sed0005150
Synteny	Sed0005150

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGATACCAAATTCAAGGGCTAGAATTTTATCATCATCAGGTGCCAGCTTCGGTTTCAAGGCTCTGATGGAGAATCAAGCTCCGAAAAAAGACTATCTCCACTATAACCACACAGATCGCTGCAGATATGCTAGATGGACTTCCAGGTTTTTCTTTCGTACATTTTCATTTGAATTTTTTATCTTCATCGTAGTTTTTGGAATCAAAATCTGTAGCTGTGAGAGCTTGTATTTGGTTGTTGTAGCAACTCAACCTGAATCTAAGAATGTAGACAATTAGGAGGTGTTTTGATGTGTAATTGCATGAATGATTATTTTGATTGATTAGAATGAGTAGTTAGTGATTATAAGTTGTGGTTCTAGTAATATCTTGTTTGGATGTGGATTTTTGTAGAGATTTTGGGAGAGGGGTTACAAAAATGAGTTATTTATTTATTATTTTAAATTTATTATTATTATTATTATTTTTTTGATATCCGTGACTGTTCGGGCCAGCTTGCGCGCACCTCGACTATTGTCATGGGGCATACCGTCTGACCCTATAACATTTGGATGTCAAGGGAAACCTGTAGGAAATTAATTCTTAGGTAGGCGGCCACCATGGGGATTGAACCCATCACCTTATTCACCCCAAAACCCTTTTGACCAATTTAGCCAATGGTTAATGAGTGTTTTTTAATGAGAGATACTTTTTAGTAATTAGAAATGAGTGGTTATAATAGCCAGTTTGTATATAACTTTTTTGAAGAGGGTTCGTAAAAGCTCATTTGTAGTAGCTGGTTAAATAATAAAAACAAACACCCTCCTTAAGCTGTTGTTTTATGCTTTTGGCAGGGAAAGCTACCAACTCATGTATGGAAGGCAGTGGCAAGAAGTGCTTGATTTTTACTCTCATGCTGTTAATGGAAGATTCTCACTGTCCTCGTTGTTTGGAACTGAGGTTAGAACCCAATATCGACCTTTTTTTTTAACTTATTTTCACTTGCAATCTTCTTTCCGAAACTTCTTTATGATTCTTAATGGAATTGTGAACTAGGAATCTTTTTCCCTCAATTGCGAGCTTGTGAATCTTTCTCTCTGTTTCTCTCGTGATCTAACTCTAAGAGCGATCTTTATATACGAATGCGAATTTTATCATAATGTTGTATCAATGTTGGGTAGTGTTTAGCTACCTCTTGATAGATATTTATGGTTACTTTCTTTTGCTTCCATCAATGGAGTCCTAAGAGTAGTTAGAAACATTTTTTTTTATAGAAAACAAAACCTTCATTGATGAATGAAAAGATTGTCCTATCGAAACATATAAAAAAACTATGCCATTAGGATAATATTGTAGATAGATCATAGTTAGAAAAGGGAGGGGTTAAAAACGATTATGAATCCTCCTCCTGCAACATAAACTAGTAGGGAAAATAGCAAGGATACTAAATTTGCTGTACGTCTTCCTAAATCCTAAGTAACTAGATTTCTTTTTGTTCAGGTCTTTGGTCCTTAAAACAATGACTCCCGTCGTAAATTGCCAATTGGTTTAACATTTTCATGTTGATGTTCTACATTTTGCTTGGATCTCAATTCTAGAGATGATTTTTTATCAAATCATGGCTATCGGTTATGTTTTTATTTGTTGATCATGTTCATGTTTTTTTTTTATAAAAAAAGTATTTTATATATTCCATTGGTTGATTACAATTAGAAAATACAAAAGGATGAGATTTTGTTTCTTTAATTTCCTCATGATTATCATCGACTGGATTTTGCAACTTTTTCATTAATCAATGTACCTGTTTCCTATATAAAAAAAAAGAAAATTTTGTTCTTTGATTATCTTTCTGTACGATTCTACAAGATATCATTGATTTATTTATTCTATATAGTACTTTATCTGCAGACAGTTCATCATGATGATGCTGCTGAGATTCAACCGATTTGCAACCCTGCAGAGTTAAGCAGAGCTTCAGTTAAAGATCGGACTGGGAGGTGGGCAAGGACAACATTTAAGATTGTTCTTTCCTACCATGGAAATTCTTTTGATGGGTGGCAAAAGCAGCCAGGGTTGGATACTGTTCAGGGGTAAGTATGTTTCAGGAGAGTGTGAACCATAGGCTCAAGTAATACACATATGTAATTGGGAGATTTTGAAACTTGGGATGTTATTATCATTATAAAGTCTTTTAAATTAAAGAATTGTTAAGATTTTGTTAGCTTTAGATAATGTTTGAGAAGTTAGTTCTAGAGATACAATTGGCGAATAGTTTTACAGAGAACTGAGTTATAGAGATATAATTGAAAATCCAATGATTCCTCTTCATGCGAACTTTCCTCTTGAGGTCAATGTCCAAACAATTTTGGGAATAGTTTGTTATAACTTTTGATTTATAAATAGTTGGATTTCATTCTAGATATGGAAAACACTCCTGTATATTAGAGGATTATTTCTACTTGTATCCTTGGGCAGTGTGGTAGAAAGATCTCTTGGGCAATTTGTAGATGAAAAGAAAGCTCAGCAGCTGAAAGATAGATGCTTACCGTTAGAAGGATGTGCTGCGGTTGCTGGGCGCACAGACAAAGGGGTGACAGCTATGCGACAAGTTTGTGCATTCTGTATGTTAGTTTTTTTAATCTCTGTTGACATGGTTTTTCTTTTTTCTTGTTTAGATATTTAATTCTTTAACTCTGCTTTTATTTTTCTAGGATTCTATCTTATTTAAAAAATGATAGTTGATAGTTGTTTGATGGGGCTAGCCATTGATTGGTGCATCAGTTACATAACAACTCTACTTATGCAGTGATTCTCTAAAACTCTCAAATAAATAACTCTATCTCATACCAACTGCTCTTGGAAGGGCATGCCAAAAGACTTGTTAAAAAAAGCAAGTAGTACTAAAACAGTAATGGGCATGACAGGCAGTAATCATCTCGTGAGTCATTACTGAAAAGATGGAATCTATGTTTTTCAATTCTCATTAAAAAGACCTATAGCTAAGGCTATTTCTTTCCTTCTTTGGTTTTGAAATCCCTTGGAGGGAAACAATGTATCTCTTTCTGACTTCACCCACTTAATTATTATACGTTTTATTTTAGAGAATCAATATCTGACTGAATTACCATCACTAGATACTTGGCGCACAGATGTTAAACCTCAAGACATTGAAGATTCCATCAATAATGCAGCTCCCGGAAAACTCAGAGTCTTATCTGTTTCTCAGGTAGTATTAAGGTATTTTATGCAAATGTATCATTATAAATGAACGTGGTTCCCCCAATATCTCTTTCAGCACATTATACATGTAACTTTTCATATCACTTACTGCTGTAGGTGTCTCGTATGTTCCATCCAAACTTCTCTGCTAAATGGAGGCGATATTTGTATATTTTTCCTCTGAATGACAGAGAGAACATGGAGCCAGATTTTGCAATTTTGGAAGAAAAATGTAGAGAAGACAATAGTTGTGATGAAATAGAAGTTGCAAATGGTGAATGCAAGGATGAGGTTCTGGATAATTTGGTCCTCAGTCGGGTTGAGGAGGAGCCTTTTTCAACTGATAAGCCTCAAACATTTAGTATAAGGAGGGTTAACCAACTACTAAGGAAATTAGAAGGAAAGTTATTGTCATACAAGATGTTTGCGCGTGACACCAAAGCATCAAGAAATGAGTGAGAATCGTGAATTGAATCAATTATTGGTTATTTTCTTTTGTTGTTATTCACTTATTCTAGCTTCTAGTTTCAGTACGATTATTCATTGTTTTTGCTATAGTTTCATAATTTCTGCTCATAAAGACAAATGCCATTTTTCTTTTTATTCTACTCATAAAACTATGAACATTTTTCTTCTTATTCTGTTAGTCCCTTGTTTGATTCTTTTTTAAGCTATTTATTATAGGAATATGAAAATGGATGAATCCAGTCTGTATGCACTTTTGTCAGATCTGTGGATAGAAAGGAACATCAGTTTTTTTCCTCATAAAATTAAGGAAAAGCAAGAGAGTTGGACAATGGAAATGTTTAAGTATCAACATTAAATTATTTGGTCCGTCCTAAGTTTAATTGTCCACCTTTTGTAATTGACTCTTCCTTGAATAGATTTAGGTCCTTCATTTGGTGGTTCTGGTTCCCCTTTTGTATTTCTCATCCTATTGAGTTCTTTTAGGGATTTGGCTTGAGTTCTTTTGAGTGGCGAAAAATGATTCAATTGAAGATAATAGATGGCTAATCTAAAAACTGACTCAATAAAGTATTGGAATGATGACTGTCCAAGTGCTTATAAAAAAAATTCTTTTCTATCACTTAAGCCATTTTTTAAGCTCTTGGAAAGTCATTTCTAACAGACGCTTAGTTAAGTTTATCATATGAGAACTTTTTGTTTGTCATTTTAGATTAATGTGCTTGTAGATATGAAGAGAAAGCTAGTCTGCTGACTGACCAAACTAAGTTAGTCAGTTTATCGGTCAAGAGCTTTTTGTTTTTGTCTGTCAACATGAGATTGATGTGCTACCCCTTGTACCTTTATTGTTCCCAAATGTTTTCTCATTAAGATAAGATGACATTTGCAACCTTTTGACATGTCCTAATTCCGACTTATCCTCCTTTTCAGAGGTCCGCCTACGGAATGTTTTGTATATCATGCTCGAGCCATGGAAGCTAGATTACCGTGCTTGGTATTTTCCTACATCCTACTTGTGGTCTTTTCGTTCTTTTGTCCTTTTCTTTGATTATATTTTTCCTTTTACACTTCTCTCCTTTTGAAATATCTTTGATAGGAGTTGTATCTGTAAATCATTCTATTCTATCCTCACTTAAACTAAAAAAAAGTTGCATTATGTTCTCTACTGGTTCAAAACATAGAGAATGGAAGATGAATATGTTGAAAAGGACTTTTAAAGCCAACTAGTCCTAGCATGAGCTTTATTCAATGCAAATTTAAAATTTATTGATAAGGGGGGTTGCATCTTCTCTTTATTTTTTTGTAGATCTTTGTTTCTTAAAATGAATACCCTTTACTTTGTTCACTTTTTCTTTTAATATCCATGAACGTTCGGGTTAACTTATGCATATCTCCACTATTCTAATGGGATGAACTACTTGACCCTAGTACATTTGGTTTTCAAGGAAATTTGTAGGAATTTAATTTCTAGGTGATGACCATAGTCACCACACCACCATGAGTTTAACTCATAACCTCTTAAATCCTCAAGACCCCCTTAGATCACTGATTCCCTAGGTTAATAATACATGATGGTTTTGTTAATTTTTTAGTTTTATTTTGCATAATAGTAAAAGCATGGTTACTAATTGTGTCCAGATGCCATTTGATATTTTCAATATTGAAGCGACTTTATTTTTTGGAATTAGAAACATTTCATTCATAAATGATAAAGGAGAAAACTTCAAGCACCTTAAGGTGAATGCAAGAAAGAGCTCTAGACGGAGAATAAAAACTAAGAAACAAAAGCCACAAATTCGTATTCATACATTAGGGCTCTTTGTGAAATCTTTTGTCTAGAGTTTCCTTATTTGTTTATCCATGTCTTAACACTCCAACAAAACTAGTGATCTGACTATGAGGTTGTATTGTATTGACAGGACCAAGGAGTAGGAAGAAGTGTAATGTGTGTTGAACTGGTTGCTAACCGTTTCTTGCGGAAGGTATCATCTCCAGGGTTACTTATGCTTAAAGATTATTATCATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTTTATAATTGGGAATTAGAGTTTTGCCACCATGCACCCGTATCCGGCTTAAAGATTATATTGTAATTTGTAACGAAAAATTGGTCTATTTAGATTAAAAAAAAAAAAAAAACATTTCCAACTGCTCAAACTTGAGAGTTACATCTTATAGCAAGGGAATTTCCCCCCGGTTTCAGCAATATGATGCAACCATATGTTTAAAGCAGTTCAAACTTAATTATAAAGGAATTTAACATGACATTGCAGATGGTGCGGGTGCTCGTTGCAACAGCAATTAGAGAGGCAGCTGCTGGTGCTGGAGAAGATGCATTACTAAAGCTGATGGATGCCACATGTCGACGTGCCACTGCACCTCCTGCGCCTCCTGATGGCCTCAGTCTTGTTGACGTAGGTTATACAGAATTTGATTCACGAAATTGCTTCATTTTAAATGACTAA

mRNA sequence

ATGATACCAAATTCAAGGGCTAGAATTTTATCATCATCAGGTGCCAGCTTCGGTTTCAAGGCTCTGATGGAGAATCAAGCTCCGAAAAAAGACTATCTCCACTATAACCACACAGATCGCTGCAGATATGCTAGATGGACTTCCAGGGAAAGCTACCAACTCATGTATGGAAGGCAGTGGCAAGAAGTGCTTGATTTTTACTCTCATGCTGTTAATGGAAGATTCTCACTGTCCTCGTTGTTTGGAACTGAGACAGTTCATCATGATGATGCTGCTGAGATTCAACCGATTTGCAACCCTGCAGAGTTAAGCAGAGCTTCAGTTAAAGATCGGACTGGGAGGTGGGCAAGGACAACATTTAAGATTGTTCTTTCCTACCATGGAAATTCTTTTGATGGGTGGCAAAAGCAGCCAGGGTTGGATACTGTTCAGGGTGTGGTAGAAAGATCTCTTGGGCAATTTGTAGATGAAAAGAAAGCTCAGCAGCTGAAAGATAGATGCTTACCGTTAGAAGGATGTGCTGCGGTTGCTGGGCGCACAGACAAAGGGGTGACAGCTATGCGACAAGTTTGTGCATTCTATACTTGGCGCACAGATGTTAAACCTCAAGACATTGAAGATTCCATCAATAATGCAGCTCCCGGAAAACTCAGAGTCTTATCTGTTTCTCAGGTGTCTCGTATGTTCCATCCAAACTTCTCTGCTAAATGGAGGCGATATTTGTATATTTTTCCTCTGAATGACAGAGAGAACATGGAGCCAGATTTTGCAATTTTGGAAGAAAAATGTAGAGAAGACAATAGTTGTGATGAAATAGAAGTTGCAAATGGTGAATGCAAGGATGAGGTTCTGGATAATTTGGTCCTCAGTCGGGTTGAGGAGGAGCCTTTTTCAACTGATAAGCCTCAAACATTTAGTATAAGGAGGGTTAACCAACTACTAAGGAAATTAGAAGGAAAGTTATTGTCATACAAGATGTTTGCGCGTGACACCAAAGCATCAAGAAATGAAGGTCCGCCTACGGAATGTTTTGTATATCATGCTCGAGCCATGGAAGCTAGATTACCGTGCTTGGACCAAGGAGTAGGAAGAAGTGTAATGTGTGTTGAACTGGTTGCTAACCGTTTCTTGCGGAAGATGGTGCGGGTGCTCGTTGCAACAGCAATTAGAGAGGCAGCTGCTGGTGCTGGAGAAGATGCATTACTAAAGCTGATGGATGCCACATGTCGACGTGCCACTGCACCTCCTGCGCCTCCTGATGGCCTCAGTCTTGTTGACGTAGGTTATACAGAATTTGATTCACGAAATTGCTTCATTTTAAATGACTAA

Coding sequence (CDS)

ATGATACCAAATTCAAGGGCTAGAATTTTATCATCATCAGGTGCCAGCTTCGGTTTCAAGGCTCTGATGGAGAATCAAGCTCCGAAAAAAGACTATCTCCACTATAACCACACAGATCGCTGCAGATATGCTAGATGGACTTCCAGGGAAAGCTACCAACTCATGTATGGAAGGCAGTGGCAAGAAGTGCTTGATTTTTACTCTCATGCTGTTAATGGAAGATTCTCACTGTCCTCGTTGTTTGGAACTGAGACAGTTCATCATGATGATGCTGCTGAGATTCAACCGATTTGCAACCCTGCAGAGTTAAGCAGAGCTTCAGTTAAAGATCGGACTGGGAGGTGGGCAAGGACAACATTTAAGATTGTTCTTTCCTACCATGGAAATTCTTTTGATGGGTGGCAAAAGCAGCCAGGGTTGGATACTGTTCAGGGTGTGGTAGAAAGATCTCTTGGGCAATTTGTAGATGAAAAGAAAGCTCAGCAGCTGAAAGATAGATGCTTACCGTTAGAAGGATGTGCTGCGGTTGCTGGGCGCACAGACAAAGGGGTGACAGCTATGCGACAAGTTTGTGCATTCTATACTTGGCGCACAGATGTTAAACCTCAAGACATTGAAGATTCCATCAATAATGCAGCTCCCGGAAAACTCAGAGTCTTATCTGTTTCTCAGGTGTCTCGTATGTTCCATCCAAACTTCTCTGCTAAATGGAGGCGATATTTGTATATTTTTCCTCTGAATGACAGAGAGAACATGGAGCCAGATTTTGCAATTTTGGAAGAAAAATGTAGAGAAGACAATAGTTGTGATGAAATAGAAGTTGCAAATGGTGAATGCAAGGATGAGGTTCTGGATAATTTGGTCCTCAGTCGGGTTGAGGAGGAGCCTTTTTCAACTGATAAGCCTCAAACATTTAGTATAAGGAGGGTTAACCAACTACTAAGGAAATTAGAAGGAAAGTTATTGTCATACAAGATGTTTGCGCGTGACACCAAAGCATCAAGAAATGAAGGTCCGCCTACGGAATGTTTTGTATATCATGCTCGAGCCATGGAAGCTAGATTACCGTGCTTGGACCAAGGAGTAGGAAGAAGTGTAATGTGTGTTGAACTGGTTGCTAACCGTTTCTTGCGGAAGATGGTGCGGGTGCTCGTTGCAACAGCAATTAGAGAGGCAGCTGCTGGTGCTGGAGAAGATGCATTACTAAAGCTGATGGATGCCACATGTCGACGTGCCACTGCACCTCCTGCGCCTCCTGATGGCCTCAGTCTTGTTGACGTAGGTTATACAGAATTTGATTCACGAAATTGCTTCATTTTAAATGACTAA

Protein sequence

MIPNSRARILSSSGASFGFKALMENQAPKKDYLHYNHTDRCRYARWTSRESYQLMYGRQWQEVLDFYSHAVNGRFSLSSLFGTETVHHDDAAEIQPICNPAELSRASVKDRTGRWARTTFKIVLSYHGNSFDGWQKQPGLDTVQGVVERSLGQFVDEKKAQQLKDRCLPLEGCAAVAGRTDKGVTAMRQVCAFYTWRTDVKPQDIEDSINNAAPGKLRVLSVSQVSRMFHPNFSAKWRRYLYIFPLNDRENMEPDFAILEEKCREDNSCDEIEVANGECKDEVLDNLVLSRVEEEPFSTDKPQTFSIRRVNQLLRKLEGKLLSYKMFARDTKASRNEGPPTECFVYHARAMEARLPCLDQGVGRSVMCVELVANRFLRKMVRVLVATAIREAAAGAGEDALLKLMDATCRRATAPPAPPDGLSLVDVGYTEFDSRNCFILND

Homology

[Close]

BLAST of Sed0005150 vs. NCBI nr
Match: XP_023006334.1 (uncharacterized protein LOC111499102 isoform X1 [Cucurbita maxima])

HSP 1 Score: 768.1 bits (1982), Expect = 4.2e-218
Identity = 384/450 (85.33%), Postives = 406/450 (90.22%), Query Frame = 0


Query: 1   MIPNSRARILSSSGASFGF------KALMENQAPKKDYLHYNHTDRCRYARWTSRESYQL 60
           M+PN RAR+LS SGA FGF      K LMEN+A KKDYLHYNHTDRCR ARWTSRESYQL
Sbjct: 43  MLPNPRARLLSPSGAGFGFGFGFGIKCLMENRAQKKDYLHYNHTDRCRNARWTSRESYQL 102

Query: 61  MYGRQWQEVLDFYSHAVNGRFSLSSLFGTETVHHDDAAEIQPICNPAELSRASVKDRTGR 120
           MY R WQEVLDFYSHAVNGR SLSSL  TETV   D  EIQP+CN AEL+RA VKD TGR
Sbjct: 103 MYERPWQEVLDFYSHAVNGRLSLSSL-RTETVRDHDDTEIQPVCNTAELNRAPVKDGTGR 162

Query: 121 WARTTFKIVLSYHGNSFDGWQKQPGLDTVQGVVERSLGQFVDEKKAQQLKDRCLPLEGCA 180
           WAR TFKIVLSYHGNSFDGWQKQP L+TVQGVVE+SLG+FVDEKKAQQLKDRCLP+EGCA
Sbjct: 163 WARATFKIVLSYHGNSFDGWQKQPELNTVQGVVEKSLGKFVDEKKAQQLKDRCLPIEGCA 222

Query: 181 AVAGRTDKGVTAMRQVCAFYTWRTDVKPQDIEDSINNAAPGKLRVLSVSQVSRMFHPNFS 240
            VAGRTDKGVTA+RQVC+FYTWRTDVKPQDIE SINNAAPGKLRVLSVSQVSRMFHPNFS
Sbjct: 223 VVAGRTDKGVTALRQVCSFYTWRTDVKPQDIEGSINNAAPGKLRVLSVSQVSRMFHPNFS 282

Query: 241 AKWRRYLYIFPLNDRENMEPDFAILE--EKCREDNSCDEIEVANGECKDEVLDNLVLSRV 300
           AKWRRYLYIFPL D ENMEP+FAILE  E  RE+   D+IEV +GECKDE+L+NL+LS+ 
Sbjct: 283 AKWRRYLYIFPLKDGENMEPNFAILEDVENSREEEYRDKIEVLHGECKDEILNNLILSQD 342

Query: 301 EEEPFSTDKPQTFSIRRVNQLLRKLEGKLLSYKMFARDTKASRNEGPPTECFVYHARAME 360
           +EEP S +KPQTFSI RVNQLLRKLEGKLLSYKMFARDTKASRNEGPPTECFVYHARAME
Sbjct: 343 DEEPASANKPQTFSIMRVNQLLRKLEGKLLSYKMFARDTKASRNEGPPTECFVYHARAME 402

Query: 361 ARLPCLDQGVGRSVMCVELVANRFLRKMVRVLVATAIREAAAGAGEDALLKLMDATCRRA 420
           ARLPCLDQGVGRSVMCVELVANRFLRKMVRVLVATAIREAAAGAGEDALLKLMDATCRRA
Sbjct: 403 ARLPCLDQGVGRSVMCVELVANRFLRKMVRVLVATAIREAAAGAGEDALLKLMDATCRRA 462

Query: 421 TAPPAPPDGLSLVDVGYTEFDSRNCFILND 443
           TAPPAPPDGLSLVDVGYTEFD +NCFILND
Sbjct: 463 TAPPAPPDGLSLVDVGYTEFDPQNCFILND 491

[Close]

BLAST of Sed0005150 vs. NCBI nr
Match: XP_022933289.1 (uncharacterized protein LOC111440432 isoform X1 [Cucurbita moschata])

HSP 1 Score: 765.0 bits (1974), Expect = 3.6e-217
Identity = 383/446 (85.87%), Postives = 403/446 (90.36%), Query Frame = 0


Query: 1   MIPNSRARILSSSGASFGF--KALMENQAPKKDYLHYNHTDRCRYARWTSRESYQLMYGR 60
           MIPN RA +LSSSGA FGF  K LMEN+A KKDYLHYNHTDRCR ARWTSRESYQ MY R
Sbjct: 1   MIPNPRAILLSSSGAGFGFGIKCLMENRAQKKDYLHYNHTDRCRNARWTSRESYQFMYER 60

Query: 61  QWQEVLDFYSHAVNGRFSLSSLFGTETVHHDDAAEIQPICNPAELSRASVKDRTGRWART 120
            WQEVLDFYSHAVNGR SLSSL GTETV   D  EIQP+CN AEL+RA  KD TGRWAR 
Sbjct: 61  PWQEVLDFYSHAVNGRLSLSSL-GTETVRDHDDTEIQPVCNTAELNRAPGKDGTGRWARA 120

Query: 121 TFKIVLSYHGNSFDGWQKQPGLDTVQGVVERSLGQFVDEKKAQQLKDRCLPLEGCAAVAG 180
           TFKIVLSYHGNSFDGWQKQP L+TVQGVVE+SLG+FVDEKKAQQLKDRCLP+EGCA VAG
Sbjct: 121 TFKIVLSYHGNSFDGWQKQPELNTVQGVVEKSLGKFVDEKKAQQLKDRCLPIEGCAVVAG 180

Query: 181 RTDKGVTAMRQVCAFYTWRTDVKPQDIEDSINNAAPGKLRVLSVSQVSRMFHPNFSAKWR 240
           RTDKGVTA+RQVC+FYTWRTDVKPQDIE SINNAAPGKLRVLSVSQVSRMFHPNFSAKWR
Sbjct: 181 RTDKGVTALRQVCSFYTWRTDVKPQDIEGSINNAAPGKLRVLSVSQVSRMFHPNFSAKWR 240

Query: 241 RYLYIFPLNDRENMEPDFAILE--EKCREDNSCDEIEVANGECKDEVLDNLVLSRVEEEP 300
           RYLYIFPL D ENMEP+FAI E  E  RE+   D+IE  +GECKDE+L+NLVLS+ +EEP
Sbjct: 241 RYLYIFPLKDGENMEPNFAIWEDVENIREEEHRDKIEALHGECKDEILNNLVLSQDDEEP 300

Query: 301 FSTDKPQTFSIRRVNQLLRKLEGKLLSYKMFARDTKASRNEGPPTECFVYHARAMEARLP 360
            S +KPQTFSI RVNQLLRKLEGKLLSYKMFARDTKASRNEGPPTECFVYHARAMEARLP
Sbjct: 301 ASANKPQTFSIMRVNQLLRKLEGKLLSYKMFARDTKASRNEGPPTECFVYHARAMEARLP 360

Query: 361 CLDQGVGRSVMCVELVANRFLRKMVRVLVATAIREAAAGAGEDALLKLMDATCRRATAPP 420
           CLDQGVGRSVMCVELVANRFLRKMVRVLVATAIREAAAGAGEDALLKLMDATCRRATAPP
Sbjct: 361 CLDQGVGRSVMCVELVANRFLRKMVRVLVATAIREAAAGAGEDALLKLMDATCRRATAPP 420

Query: 421 APPDGLSLVDVGYTEFDSRNCFILND 443
           APPDGLSLVDVGYTEFD +NCFILND
Sbjct: 421 APPDGLSLVDVGYTEFDPQNCFILND 445

[Close]

BLAST of Sed0005150 vs. NCBI nr
Match: XP_023531012.1 (uncharacterized protein LOC111793396 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 763.8 bits (1971), Expect = 7.9e-217
Identity = 384/446 (86.10%), Postives = 404/446 (90.58%), Query Frame = 0


Query: 1   MIPNSRARILSSSGASFGF--KALMENQAPKKDYLHYNHTDRCRYARWTSRESYQLMYGR 60
           MIPN RAR+LS SGA FGF  K LMEN+A KKDYLHYNHTDRCR ARWTSRESYQ MY R
Sbjct: 1   MIPNPRARLLSPSGAGFGFGIKCLMENRAQKKDYLHYNHTDRCRNARWTSRESYQFMYER 60

Query: 61  QWQEVLDFYSHAVNGRFSLSSLFGTETVHHDDAAEIQPICNPAELSRASVKDRTGRWART 120
            WQEVLDFYSHAVNGR SLSSL GTETV   D  EIQP+CN AEL+RA VKD TGRWAR 
Sbjct: 61  PWQEVLDFYSHAVNGRLSLSSL-GTETVRDHDDTEIQPVCNTAELNRAPVKDGTGRWARA 120

Query: 121 TFKIVLSYHGNSFDGWQKQPGLDTVQGVVERSLGQFVDEKKAQQLKDRCLPLEGCAAVAG 180
           TFKIVLSYHGNSFDGWQKQP L TVQGVVE+SLG+FVDEKKAQQLKDRCLP+EGCA VAG
Sbjct: 121 TFKIVLSYHGNSFDGWQKQPELITVQGVVEKSLGKFVDEKKAQQLKDRCLPIEGCAVVAG 180

Query: 181 RTDKGVTAMRQVCAFYTWRTDVKPQDIEDSINNAAPGKLRVLSVSQVSRMFHPNFSAKWR 240
           RTDKGVTA+RQVC+FYTWRTDVKPQDIE SINNAAPGKLRVLSVSQVSRMFHPNFSAKWR
Sbjct: 181 RTDKGVTALRQVCSFYTWRTDVKPQDIEGSINNAAPGKLRVLSVSQVSRMFHPNFSAKWR 240

Query: 241 RYLYIFPLNDRENMEPDFAILE--EKCREDNSCDEIEVANGECKDEVLDNLVLSRVEEEP 300
           RYLYIFPL D ENMEP+F I E  E  RE++  D+IEV +GECKDE+L+NLVLS+ +EEP
Sbjct: 241 RYLYIFPLKDGENMEPNFPIWEDVENSREEDR-DKIEVLHGECKDEILNNLVLSQDDEEP 300

Query: 301 FSTDKPQTFSIRRVNQLLRKLEGKLLSYKMFARDTKASRNEGPPTECFVYHARAMEARLP 360
            S +KPQTFSI RVNQLLRKLEGKLLSYKMFARDTKASRNEGPPTECFVYHARAMEARLP
Sbjct: 301 ASANKPQTFSIMRVNQLLRKLEGKLLSYKMFARDTKASRNEGPPTECFVYHARAMEARLP 360

Query: 361 CLDQGVGRSVMCVELVANRFLRKMVRVLVATAIREAAAGAGEDALLKLMDATCRRATAPP 420
           CLDQGVGRSVMCVELVANRFLRKMVRVLVATAIREAAAGAGEDALLKLMDATCRRATAPP
Sbjct: 361 CLDQGVGRSVMCVELVANRFLRKMVRVLVATAIREAAAGAGEDALLKLMDATCRRATAPP 420

Query: 421 APPDGLSLVDVGYTEFDSRNCFILND 443
           APPDGLSLVDVGYTEFD +NCFILND
Sbjct: 421 APPDGLSLVDVGYTEFDPQNCFILND 444

[Close]

BLAST of Sed0005150 vs. NCBI nr
Match: XP_038878348.1 (tRNA pseudouridine synthase A [Benincasa hispida])

HSP 1 Score: 763.1 bits (1969), Expect = 1.4e-216
Identity = 376/445 (84.49%), Postives = 404/445 (90.79%), Query Frame = 0


Query: 1   MIPNSRARILSSSGASFGFKALMENQAPKKDYLHYNHTDRCRYARWTSRESYQLMYGRQW 60
           MIPN RAR++S SGA FGFK LMEN+A +KDYLHYNHTDRCR ARWT+RESYQLMYGR W
Sbjct: 1   MIPNPRARLVSPSGAGFGFKILMENRAQQKDYLHYNHTDRCRNARWTARESYQLMYGRPW 60

Query: 61  QEVLDFYSHAVNGRFSLSSLFGTETVHHDDAAEIQPICNPAELSRASVKDRTGRWARTTF 120
           QEVLDFY HAVNGRFSLSSLFGTE  H  D AEI  I N AEL+R SVKD+TGRWAR TF
Sbjct: 61  QEVLDFYFHAVNGRFSLSSLFGTEIGHDHDDAEIPLIYNTAELNRVSVKDKTGRWARATF 120

Query: 121 KIVLSYHGNSFDGWQKQPGLDTVQGVVERSLGQFVDEKKAQQLKDRCLPLEGCAAVAGRT 180
           KIVLSYHGNSFDGWQKQPGL+TVQG+VE+SLG+FVD+KKAQ LKD+CLP+EGCA VAGRT
Sbjct: 121 KIVLSYHGNSFDGWQKQPGLNTVQGLVEKSLGKFVDDKKAQMLKDKCLPIEGCAVVAGRT 180

Query: 181 DKGVTAMRQVCAFYTWRTDVKPQDIEDSINNAAPGKLRVLSVSQVSRMFHPNFSAKWRRY 240
           DKGVTA+RQVC+FYTWRTDV+PQDIEDSIN AAPGKLR LSVSQVSR+FHPNFSAKWRRY
Sbjct: 181 DKGVTALRQVCSFYTWRTDVQPQDIEDSINRAAPGKLRTLSVSQVSRIFHPNFSAKWRRY 240

Query: 241 LYIFPLNDRENMEPDFAILE--EKCREDNSCDEIEVANG-ECKDEVLDNLVLSRVEEEPF 300
           LYIFPLND ENMEPD+AI E  E CR++ +  +IEV NG ECKDE+ DNLV    +EE  
Sbjct: 241 LYIFPLNDGENMEPDYAIWEDVEDCRKEENYGKIEVLNGEECKDEIHDNLVFGPNDEELV 300

Query: 301 STDKPQTFSIRRVNQLLRKLEGKLLSYKMFARDTKASRNEGPPTECFVYHARAMEARLPC 360
           S DKPQTFSIRRVNQLLRKLEGKLLSYKMFARDTKASRNEGPPTECFVYHARA+EARLPC
Sbjct: 301 SADKPQTFSIRRVNQLLRKLEGKLLSYKMFARDTKASRNEGPPTECFVYHARAVEARLPC 360

Query: 361 LDQGVGRSVMCVELVANRFLRKMVRVLVATAIREAAAGAGEDALLKLMDATCRRATAPPA 420
           LDQGVGRSVMCVELVANRFLRKMVRVLVATAIREAAAGAGEDALL+LMDATCRRATAPPA
Sbjct: 361 LDQGVGRSVMCVELVANRFLRKMVRVLVATAIREAAAGAGEDALLELMDATCRRATAPPA 420

Query: 421 PPDGLSLVDVGYTEFDSRNCFILND 443
           PPDGLSLVDVGYTEFDS+NCFILND
Sbjct: 421 PPDGLSLVDVGYTEFDSQNCFILND 445

[Close]

BLAST of Sed0005150 vs. NCBI nr
Match: KAG7021669.1 (truA, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 763.1 bits (1969), Expect = 1.4e-216
Identity = 382/446 (85.65%), Postives = 401/446 (89.91%), Query Frame = 0


Query: 1   MIPNSRARILSSSGASFGF--KALMENQAPKKDYLHYNHTDRCRYARWTSRESYQLMYGR 60
           MIPN RA +LSSSGA FGF  K LMEN+A KKDYLHYNHTDRCR ARWTSRESYQ MY R
Sbjct: 43  MIPNPRAILLSSSGAGFGFGIKCLMENRAQKKDYLHYNHTDRCRNARWTSRESYQFMYER 102

Query: 61  QWQEVLDFYSHAVNGRFSLSSLFGTETVHHDDAAEIQPICNPAELSRASVKDRTGRWART 120
            WQEVLDFYSHAVNGR SLSSL GTETV   D  EIQP+CN AEL RA VKD TGRWAR 
Sbjct: 103 PWQEVLDFYSHAVNGRLSLSSL-GTETVRDHDDTEIQPVCNTAELDRAPVKDGTGRWARA 162

Query: 121 TFKIVLSYHGNSFDGWQKQPGLDTVQGVVERSLGQFVDEKKAQQLKDRCLPLEGCAAVAG 180
           TFKIVLSYHGNSFDGWQKQP L+TVQGVVE+SLG+FVDEKKAQQLKDRCLP+EGCA VAG
Sbjct: 163 TFKIVLSYHGNSFDGWQKQPDLNTVQGVVEKSLGKFVDEKKAQQLKDRCLPIEGCAVVAG 222

Query: 181 RTDKGVTAMRQVCAFYTWRTDVKPQDIEDSINNAAPGKLRVLSVSQVSRMFHPNFSAKWR 240
           RTDKGVTA+RQVC+FYTWRTDVKPQDIE SINNAAPGKLRVLSVSQVSRMFHPNFSAKWR
Sbjct: 223 RTDKGVTALRQVCSFYTWRTDVKPQDIEGSINNAAPGKLRVLSVSQVSRMFHPNFSAKWR 282

Query: 241 RYLYIFPLNDRENMEPDFAILE--EKCREDNSCDEIEVANGECKDEVLDNLVLSRVEEEP 300
           RYLYIFPL D E MEP+FAI E  E  RE+   D+IE  +GECKDE+L+NLVLS+ +EEP
Sbjct: 283 RYLYIFPLKDGEKMEPNFAIWEDVENSREEEHRDKIEALHGECKDEILNNLVLSQDDEEP 342

Query: 301 FSTDKPQTFSIRRVNQLLRKLEGKLLSYKMFARDTKASRNEGPPTECFVYHARAMEARLP 360
              +KPQTFSI RVNQLLRKLEGKLLSYKMFARDTKASRNEGPPTECFVYHARAMEARLP
Sbjct: 343 ALANKPQTFSIMRVNQLLRKLEGKLLSYKMFARDTKASRNEGPPTECFVYHARAMEARLP 402

Query: 361 CLDQGVGRSVMCVELVANRFLRKMVRVLVATAIREAAAGAGEDALLKLMDATCRRATAPP 420
           CLDQGVGRSVMCVELVANRFLRKMVRVLVATAIREAAAGAGEDALLKLMDATCRRATAPP
Sbjct: 403 CLDQGVGRSVMCVELVANRFLRKMVRVLVATAIREAAAGAGEDALLKLMDATCRRATAPP 462

Query: 421 APPDGLSLVDVGYTEFDSRNCFILND 443
           APPDGLSLVDVGYTEFD +NCFILND
Sbjct: 463 APPDGLSLVDVGYTEFDPQNCFILND 487

[Close]

BLAST of Sed0005150 vs. ExPASy Swiss-Prot
Match: Q01WL4 (tRNA pseudouridine synthase A OS=Solibacter usitatus (strain Ellin6076) OX=234267 GN=truA PE=3 SV=1)

HSP 1 Score: 75.1 bits (183), Expect = 2.2e-12
Identity = 81/309 (26.21%), Postives = 115/309 (37.22%), Query Frame = 0


Query: 121 KIVLSYHGNSFDGWQKQPGLDTVQGVVERSLGQFVDEKKAQQLKDRCLPLEGCAAVAGRT 180
           +I L+Y G  F GWQ QPGL T+QG++E  +G    +           P+    A +GRT
Sbjct: 5   RIYLAYDGGPFHGWQVQPGLATIQGILEEIVGGMEGK-----------PVH--VAGSGRT 64

Query: 181 DKGVTAMRQVCAFYTWRTDVKPQDIEDSINNAAPGKLRVLSVSQVSRMFHPNFSAKWRRY 240
           D GV A+ QV AF +    +  +++  ++N   P  +R+L   +V   FHP F A  + Y
Sbjct: 65  DAGVHALAQVAAF-SIENPIPVENLRRAVNRLLPPAIRILCAEEVQADFHPRFDAVAKTY 124

Query: 241 LYIFPLNDRENMEPDFAILEEKCREDNSCDEIEVANGECKDEVLDNLVLSRVEEEPFSTD 300
            Y                                     +DEV        V   P+  D
Sbjct: 125 EYRM----------------------------------FRDEVCSPFEWPYVYHHPYPLD 184

Query: 301 KPQTFSIRRVNQLLRKLEGKLLSYKMFARDTKASRNEGPPTECFVYHARAMEARLPCLDQ 360
           +       R+  L R  EG+       A D + +  +      F         RL    +
Sbjct: 185 E------ARMATLARAFEGEHDFTPFAASDARDAEGKSKVRTIFSSTLDRTPQRLVYRVR 240

Query: 361 GVGRSVMCVELVANRFLRKMVRVLVATAIREAAAGAGEDALLKLMDATCRRATAPPAPPD 420
           G G            FL+ MVR +V T I    AG G  A L  + A     +   AP  
Sbjct: 245 GSG------------FLKHMVRNIVGTLIE---AGRGNIADLNSLPA----RSGATAPAK 240

Query: 421 GLSLVDVGY 430
           GL  V V Y
Sbjct: 305 GLFQVSVEY 240

[Close]

BLAST of Sed0005150 vs. ExPASy Swiss-Prot
Match: B1LB83 (tRNA pseudouridine synthase A OS=Thermotoga sp. (strain RQ2) OX=126740 GN=truA PE=3 SV=1)

HSP 1 Score: 75.1 bits (183), Expect = 2.2e-12
Identity = 44/130 (33.85%), Postives = 68/130 (52.31%), Query Frame = 0


Query: 123 VLSYHGNSFDGWQKQPGLDTVQGVVERSLGQFVDEKKAQQLKDRCLPLEGCAAVAGRTDK 182
           V+ Y G++F G+Q QP + TVQGV+E +L +   ++   Q              AGRTD 
Sbjct: 7   VIEYDGSNFFGYQGQPDVRTVQGVIEDALERIFKQRIYTQ-------------AAGRTDA 66

Query: 183 GVTAMRQVCAFYTWRTDVKPQDIEDSINNAAPGKLRVLSVSQVSRMFHPNFSAKWRRYLY 242
           GV A  Q+ AF      +  +DI++++N   P  + V  V +VS+ FHP F  K R Y Y
Sbjct: 67  GVHANGQLIAFNCPNDRMTTEDIKNAMNANLPDDVYVKEVFEVSKNFHPRFDVKKRIYHY 123

Query: 243 IFPLNDRENM 253
               + ++N+
Sbjct: 127 FILTSKQKNV 123

[Close]

BLAST of Sed0005150 vs. ExPASy Swiss-Prot
Match: Q73KE3 (tRNA pseudouridine synthase A OS=Treponema denticola (strain ATCC 35405 / DSM 14222 / CIP 103919 / JCM 8153 / KCTC 15104) OX=243275 GN=truA PE=3 SV=1)

HSP 1 Score: 75.1 bits (183), Expect = 2.2e-12
Identity = 79/322 (24.53%), Postives = 124/322 (38.51%), Query Frame = 0


Query: 122 IVLSYHGNSFDGWQKQ-----PGLDTVQGVVERSLGQFVDEKKAQQLKDRCLPLEGCAAV 181
           + +SY G +F GWQKQ         TVQG +E++L            K    P+E     
Sbjct: 12  LTISYDGTNFCGWQKQTKEGAETFRTVQGELEKAL-----------TKIHKHPIE--TNG 71

Query: 182 AGRTDKGVTAMRQVCAFYTWRTDVKPQDIEDSINNAAPGKLRVLSVSQVSRMFHPNFSAK 241
           +GRTD GV A RQ   F+     ++  +   ++N+  P  +RV+  ++VS + H  F+A 
Sbjct: 72  SGRTDSGVHAARQAVNFFCDIKSMRASNFLPALNSILPKDIRVMDAAEVSPLLHARFNAL 131

Query: 242 WRRYLYIFPLNDRENMEPDFAILEEKCREDNSCDEIEVANGECKDEVLDNLVLSRVEEEP 301
            R Y Y                 + KC                              +  
Sbjct: 132 SRTYRY-----------------KIKC-----------------------------GKTI 191

Query: 302 FSTDKPQTFSIRR------VNQLLRKLEGKL-LSYKMFARDTKASRNEGPPTECFVYHAR 361
           F+ ++P T+ IRR      +N++   L G+L  +    A D   S++       F     
Sbjct: 192 FAHEQPYTWHIRRYPDIAALNEMASCLSGELDCTAFSAAGDQSISKSRYIKKAVFF---- 251

Query: 362 AMEARLPCLDQGVGRSVMCVELVANRFLRKMVRVLVATAIREAAAGAGEDALLKLMDATC 421
                       +    +  E+ AN FL KMVR +V T +     GA +     ++++  
Sbjct: 252 ------------IENDYLIFEICANAFLWKMVRSIVGTLLHLDEIGASKKDFKDILESKM 258

Query: 422 RRATAPPAPPDGLSLVDVGYTE 432
           R    P APP GL L  + Y E
Sbjct: 312 REKAGPTAPPQGLFLWSIEYPE 258

[Close]

BLAST of Sed0005150 vs. ExPASy Swiss-Prot
Match: B9K8Q0 (tRNA pseudouridine synthase A OS=Thermotoga neapolitana (strain ATCC 49049 / DSM 4359 / NBRC 107923 / NS-E) OX=309803 GN=truA PE=3 SV=1)

HSP 1 Score: 73.6 bits (179), Expect = 6.5e-12
Identity = 71/300 (23.67%), Postives = 119/300 (39.67%), Query Frame = 0


Query: 123 VLSYHGNSFDGWQKQPGLDTVQGVVERSLGQFVDEKKAQQLKDRCLPLEGCAAVAGRTDK 182
           ++ Y G++F G+Q QP + TVQGV+E +L +   ++   Q              AGRTD 
Sbjct: 7   IVEYDGSNFFGYQGQPDVRTVQGVIEDALERIFKQRIYTQ-------------AAGRTDA 66

Query: 183 GVTAMRQVCAFYTWRTDVKPQDIEDSINNAAPGKLRVLSVSQVSRMFHPNFSAKWRRYLY 242
           GV A  Q+ AF      +  +DI++++N   P  + V  V +V + FHP F  K R Y Y
Sbjct: 67  GVHANGQLIAFNCPNDRMTTEDIKNAMNANLPDDVYVKKVFEVPKNFHPRFDVKKRIYHY 126

Query: 243 IFPLNDRENMEPDFAILEEKCREDNSCDEIEVANGECKDEVLDNLVLSRVEEEPFSTDKP 302
               +  +N                                        V    ++   P
Sbjct: 127 FIHTSREKN----------------------------------------VFLRKYAWWFP 186

Query: 303 QTFSIRRVNQLLRKLEGKLLSYKMFARDTKASRNEGPPTECFVYHARAMEARLPCLDQGV 362
               +  + +  + LEG    +  F    K   +E  P    +Y  R +          +
Sbjct: 187 YELDLEAMRKAAKYLEG-THDFTSF----KTGSDERDPVRT-IYRIRIL---------SL 238

Query: 363 GRSVMCVELVANRFLRKMVRVLVATAIREAAAGAGEDALLKLMDATCRRATAPPAPPDGL 422
           G+ ++ + +    FLR+MVR +VA  ++        + L ++++A  R A A  AP  GL
Sbjct: 247 GKDMILIRVEGRSFLRRMVRNIVAALVKVGLRQWEPEKLKEVLEARDRSAAAGTAPAHGL 238

[Close]

BLAST of Sed0005150 vs. ExPASy Swiss-Prot
Match: B7K222 (tRNA pseudouridine synthase A OS=Rippkaea orientalis (strain PCC 8801) OX=41431 GN=truA PE=3 SV=1)

HSP 1 Score: 72.8 bits (177), Expect = 1.1e-11
Identity = 44/131 (33.59%), Postives = 63/131 (48.09%), Query Frame = 0


Query: 122 IVLSYHGNSFDGWQKQPGLDTVQGVVERSLGQFVDEKKAQQLKDRCLPLEGCAAVAGRTD 181
           +V+ Y G+ F GWQ QP   TVQ  +E ++ + + E          + L G    AGRTD
Sbjct: 14  LVIQYLGSRFHGWQWQPNQRTVQAEIENAIAEVLGE---------TVTLHG----AGRTD 73

Query: 182 KGVTAMRQVCAFYTWRTDVKPQDIEDSINNAAPGKLRVLSVSQVSRMFHPNFSAKWRRYL 241
            GV A  QV A +   + +      D +N   P  + +   +QV   +H  FSA+WRRY 
Sbjct: 74  AGVHAAAQV-AHFDMVSPIPASRWVDILNARLPEDILIRGSAQVPLTWHARFSAQWRRYR 130

Query: 242 YIFPLNDRENM 253
           Y F    R N+
Sbjct: 134 YTFYTEQRPNL 130

[Close]

BLAST of Sed0005150 vs. ExPASy TrEMBL
Match: A0A6J1L1W3 (tRNA pseudouridine synthase OS=Cucurbita maxima OX=3661 GN=LOC111499102 PE=3 SV=1)

HSP 1 Score: 768.1 bits (1982), Expect = 2.0e-218
Identity = 384/450 (85.33%), Postives = 406/450 (90.22%), Query Frame = 0


Query: 1   MIPNSRARILSSSGASFGF------KALMENQAPKKDYLHYNHTDRCRYARWTSRESYQL 60
           M+PN RAR+LS SGA FGF      K LMEN+A KKDYLHYNHTDRCR ARWTSRESYQL
Sbjct: 43  MLPNPRARLLSPSGAGFGFGFGFGIKCLMENRAQKKDYLHYNHTDRCRNARWTSRESYQL 102

Query: 61  MYGRQWQEVLDFYSHAVNGRFSLSSLFGTETVHHDDAAEIQPICNPAELSRASVKDRTGR 120
           MY R WQEVLDFYSHAVNGR SLSSL  TETV   D  EIQP+CN AEL+RA VKD TGR
Sbjct: 103 MYERPWQEVLDFYSHAVNGRLSLSSL-RTETVRDHDDTEIQPVCNTAELNRAPVKDGTGR 162

Query: 121 WARTTFKIVLSYHGNSFDGWQKQPGLDTVQGVVERSLGQFVDEKKAQQLKDRCLPLEGCA 180
           WAR TFKIVLSYHGNSFDGWQKQP L+TVQGVVE+SLG+FVDEKKAQQLKDRCLP+EGCA
Sbjct: 163 WARATFKIVLSYHGNSFDGWQKQPELNTVQGVVEKSLGKFVDEKKAQQLKDRCLPIEGCA 222

Query: 181 AVAGRTDKGVTAMRQVCAFYTWRTDVKPQDIEDSINNAAPGKLRVLSVSQVSRMFHPNFS 240
            VAGRTDKGVTA+RQVC+FYTWRTDVKPQDIE SINNAAPGKLRVLSVSQVSRMFHPNFS
Sbjct: 223 VVAGRTDKGVTALRQVCSFYTWRTDVKPQDIEGSINNAAPGKLRVLSVSQVSRMFHPNFS 282

Query: 241 AKWRRYLYIFPLNDRENMEPDFAILE--EKCREDNSCDEIEVANGECKDEVLDNLVLSRV 300
           AKWRRYLYIFPL D ENMEP+FAILE  E  RE+   D+IEV +GECKDE+L+NL+LS+ 
Sbjct: 283 AKWRRYLYIFPLKDGENMEPNFAILEDVENSREEEYRDKIEVLHGECKDEILNNLILSQD 342

Query: 301 EEEPFSTDKPQTFSIRRVNQLLRKLEGKLLSYKMFARDTKASRNEGPPTECFVYHARAME 360
           +EEP S +KPQTFSI RVNQLLRKLEGKLLSYKMFARDTKASRNEGPPTECFVYHARAME
Sbjct: 343 DEEPASANKPQTFSIMRVNQLLRKLEGKLLSYKMFARDTKASRNEGPPTECFVYHARAME 402

Query: 361 ARLPCLDQGVGRSVMCVELVANRFLRKMVRVLVATAIREAAAGAGEDALLKLMDATCRRA 420
           ARLPCLDQGVGRSVMCVELVANRFLRKMVRVLVATAIREAAAGAGEDALLKLMDATCRRA
Sbjct: 403 ARLPCLDQGVGRSVMCVELVANRFLRKMVRVLVATAIREAAAGAGEDALLKLMDATCRRA 462

Query: 421 TAPPAPPDGLSLVDVGYTEFDSRNCFILND 443
           TAPPAPPDGLSLVDVGYTEFD +NCFILND
Sbjct: 463 TAPPAPPDGLSLVDVGYTEFDPQNCFILND 491

[Close]

BLAST of Sed0005150 vs. ExPASy TrEMBL
Match: A0A6J1EZC2 (tRNA pseudouridine synthase OS=Cucurbita moschata OX=3662 GN=LOC111440432 PE=3 SV=1)

HSP 1 Score: 765.0 bits (1974), Expect = 1.7e-217
Identity = 383/446 (85.87%), Postives = 403/446 (90.36%), Query Frame = 0


Query: 1   MIPNSRARILSSSGASFGF--KALMENQAPKKDYLHYNHTDRCRYARWTSRESYQLMYGR 60
           MIPN RA +LSSSGA FGF  K LMEN+A KKDYLHYNHTDRCR ARWTSRESYQ MY R
Sbjct: 1   MIPNPRAILLSSSGAGFGFGIKCLMENRAQKKDYLHYNHTDRCRNARWTSRESYQFMYER 60

Query: 61  QWQEVLDFYSHAVNGRFSLSSLFGTETVHHDDAAEIQPICNPAELSRASVKDRTGRWART 120
            WQEVLDFYSHAVNGR SLSSL GTETV   D  EIQP+CN AEL+RA  KD TGRWAR 
Sbjct: 61  PWQEVLDFYSHAVNGRLSLSSL-GTETVRDHDDTEIQPVCNTAELNRAPGKDGTGRWARA 120

Query: 121 TFKIVLSYHGNSFDGWQKQPGLDTVQGVVERSLGQFVDEKKAQQLKDRCLPLEGCAAVAG 180
           TFKIVLSYHGNSFDGWQKQP L+TVQGVVE+SLG+FVDEKKAQQLKDRCLP+EGCA VAG
Sbjct: 121 TFKIVLSYHGNSFDGWQKQPELNTVQGVVEKSLGKFVDEKKAQQLKDRCLPIEGCAVVAG 180

Query: 181 RTDKGVTAMRQVCAFYTWRTDVKPQDIEDSINNAAPGKLRVLSVSQVSRMFHPNFSAKWR 240
           RTDKGVTA+RQVC+FYTWRTDVKPQDIE SINNAAPGKLRVLSVSQVSRMFHPNFSAKWR
Sbjct: 181 RTDKGVTALRQVCSFYTWRTDVKPQDIEGSINNAAPGKLRVLSVSQVSRMFHPNFSAKWR 240

Query: 241 RYLYIFPLNDRENMEPDFAILE--EKCREDNSCDEIEVANGECKDEVLDNLVLSRVEEEP 300
           RYLYIFPL D ENMEP+FAI E  E  RE+   D+IE  +GECKDE+L+NLVLS+ +EEP
Sbjct: 241 RYLYIFPLKDGENMEPNFAIWEDVENIREEEHRDKIEALHGECKDEILNNLVLSQDDEEP 300

Query: 301 FSTDKPQTFSIRRVNQLLRKLEGKLLSYKMFARDTKASRNEGPPTECFVYHARAMEARLP 360
            S +KPQTFSI RVNQLLRKLEGKLLSYKMFARDTKASRNEGPPTECFVYHARAMEARLP
Sbjct: 301 ASANKPQTFSIMRVNQLLRKLEGKLLSYKMFARDTKASRNEGPPTECFVYHARAMEARLP 360

Query: 361 CLDQGVGRSVMCVELVANRFLRKMVRVLVATAIREAAAGAGEDALLKLMDATCRRATAPP 420
           CLDQGVGRSVMCVELVANRFLRKMVRVLVATAIREAAAGAGEDALLKLMDATCRRATAPP
Sbjct: 361 CLDQGVGRSVMCVELVANRFLRKMVRVLVATAIREAAAGAGEDALLKLMDATCRRATAPP 420

Query: 421 APPDGLSLVDVGYTEFDSRNCFILND 443
           APPDGLSLVDVGYTEFD +NCFILND
Sbjct: 421 APPDGLSLVDVGYTEFDPQNCFILND 445

[Close]

BLAST of Sed0005150 vs. ExPASy TrEMBL
Match: A0A6J1C0W1 (tRNA pseudouridine synthase OS=Momordica charantia OX=3673 GN=LOC111007251 PE=3 SV=1)

HSP 1 Score: 744.2 bits (1920), Expect = 3.1e-211
Identity = 374/446 (83.86%), Postives = 403/446 (90.36%), Query Frame = 0


Query: 1   MIPNSRARILSSSGASFGFKALMENQAPKKDYLHYNHTDRCRYARWTSRESYQLMYGRQW 60
           MIPN RAR++SS  A FG K LMEN+AP+KDYLHYNHTDRCR ARWT+RESYQLMYGR W
Sbjct: 1   MIPNPRARLVSS--ADFGCKFLMENRAPQKDYLHYNHTDRCRNARWTARESYQLMYGRPW 60

Query: 61  QEVLDFYSHAVNGRFSLSSLFGTE--TVHHDDAAEIQPICNPAELSRASVKDRTGRWART 120
           QEVLDFY HAVNGRFS SSLFGTE  TVH  + AEI  I N +EL+RASVKD+TGRWAR 
Sbjct: 61  QEVLDFYFHAVNGRFSPSSLFGTEVKTVHDHNDAEIPLIYNTSELNRASVKDKTGRWARA 120

Query: 121 TFKIVLSYHGNSFDGWQKQPGLDTVQGVVERSLGQFVDEKKAQQLKDRCLPLEGCAAVAG 180
           TFKIVLSYHGNSFDGWQKQPGL+TVQG+VE+SLG+FVDEKKAQ LKDRCLPLEGCA VAG
Sbjct: 121 TFKIVLSYHGNSFDGWQKQPGLNTVQGLVEKSLGKFVDEKKAQLLKDRCLPLEGCAVVAG 180

Query: 181 RTDKGVTAMRQVCAFYTWRTDVKPQDIEDSINNAAPGKLRVLSVSQVSRMFHPNFSAKWR 240
           RTDKGVTA+RQVC+FYTWRTDVKP DIEDSIN+AAP KLRVLSVSQVSR FHPNFSAKWR
Sbjct: 181 RTDKGVTALRQVCSFYTWRTDVKPLDIEDSINSAAPRKLRVLSVSQVSRNFHPNFSAKWR 240

Query: 241 RYLYIFPLNDRENMEPDFAILE--EKCREDNSCDEIEVANGECKDEVLDNLVLSRVEEEP 300
           RYLYIFPL D ENMEPDFAI E  E CRE+ + ++IE  NGE KDE+LDNLVLS+  EE 
Sbjct: 241 RYLYIFPLKDGENMEPDFAIGEDAENCREEENYNKIEALNGEFKDEILDNLVLSQ-NEEL 300

Query: 301 FSTDKPQTFSIRRVNQLLRKLEGKLLSYKMFARDTKASRNEGPPTECFVYHARAMEARLP 360
            S DKPQTFS+RRVN+LLRKLEGKLLSYKMFARDTKASRNEGPPTECF+YHARA+EARLP
Sbjct: 301 ASDDKPQTFSVRRVNRLLRKLEGKLLSYKMFARDTKASRNEGPPTECFLYHARAVEARLP 360

Query: 361 CLDQGVGRSVMCVELVANRFLRKMVRVLVATAIREAAAGAGEDALLKLMDATCRRATAPP 420
           CLDQGVGRSVMCVELVANRFLRKMVRVLVATAIRE AAGAGEDALL+LMDATCRRATAPP
Sbjct: 361 CLDQGVGRSVMCVELVANRFLRKMVRVLVATAIREVAAGAGEDALLRLMDATCRRATAPP 420

Query: 421 APPDGLSLVDVGYTEFDSRNCFILND 443
           APPDGLSLVDVGY+EFD +NCFILND
Sbjct: 421 APPDGLSLVDVGYSEFDPQNCFILND 443

[Close]

BLAST of Sed0005150 vs. ExPASy TrEMBL
Match: A0A6J1L1X2 (tRNA pseudouridine synthase OS=Cucurbita maxima OX=3661 GN=LOC111499102 PE=3 SV=1)

HSP 1 Score: 738.0 bits (1904), Expect = 2.3e-209
Identity = 373/442 (84.39%), Postives = 394/442 (89.14%), Query Frame = 0


Query: 1   MIPNSRARILSSSGASFGF------KALMENQAPKKDYLHYNHTDRCRYARWTSRESYQL 60
           M+PN RAR+LS SGA FGF      K LMEN+A KKDYLHYNHTDRCR ARWTSRESYQL
Sbjct: 43  MLPNPRARLLSPSGAGFGFGFGFGIKCLMENRAQKKDYLHYNHTDRCRNARWTSRESYQL 102

Query: 61  MYGRQWQEVLDFYSHAVNGRFSLSSLFGTETVHHDDAAEIQPICNPAELSRASVKDRTGR 120
           MY R WQEVLDFYSHAVNGR SLSSL  TETV   D  EIQP+CN AEL+RA VKD TGR
Sbjct: 103 MYERPWQEVLDFYSHAVNGRLSLSSL-RTETVRDHDDTEIQPVCNTAELNRAPVKDGTGR 162

Query: 121 WARTTFKIVLSYHGNSFDGWQKQPGLDTVQGVVERSLGQFVDEKKAQQLKDRCLPLEGCA 180
           WAR TFKIVLSYHGNSFDGWQKQP L+TVQGVVE+SLG+FVDEKKAQQLKDRCLP+EGCA
Sbjct: 163 WARATFKIVLSYHGNSFDGWQKQPELNTVQGVVEKSLGKFVDEKKAQQLKDRCLPIEGCA 222

Query: 181 AVAGRTDKGVTAMRQVCAFYTWRTDVKPQDIEDSINNAAPGKLRVLSVSQVSRMFHPNFS 240
            VAGRTDKGVTA+RQVC+FYTWRTDVKPQDIE SINNAAPGKLRVLSVSQVSRMFHPNFS
Sbjct: 223 VVAGRTDKGVTALRQVCSFYTWRTDVKPQDIEGSINNAAPGKLRVLSVSQVSRMFHPNFS 282

Query: 241 AKWRRYLYIFPLNDRENMEPDFAILE--EKCREDNSCDEIEVANGECKDEVLDNLVLSRV 300
           AKWRRYLYIFPL D ENMEP+FAILE  E  RE+   D+IEV +GECKDE+L+NL+LS+ 
Sbjct: 283 AKWRRYLYIFPLKDGENMEPNFAILEDVENSREEEYRDKIEVLHGECKDEILNNLILSQD 342

Query: 301 EEEPFSTDKPQTFSIRRVNQLLRKLEGKLLSYKMFARDTKASRNEGPPTECFVYHARAME 360
           +EEP S +KPQTFSI RVNQLLRKLEGKLLSYKMFARDTKASRNEGPPTECFVYHARAME
Sbjct: 343 DEEPASANKPQTFSIMRVNQLLRKLEGKLLSYKMFARDTKASRNEGPPTECFVYHARAME 402

Query: 361 ARLPCLDQGVGRSVMCVELVANRFLRKMVRVLVATAIREAAAGAGEDALLKLMDATCRRA 420
           ARLPCLDQGVGRSVMCVELVANRFLRKMVRVLVATAIREAAAGAGEDALLKLMDATCRRA
Sbjct: 403 ARLPCLDQGVGRSVMCVELVANRFLRKMVRVLVATAIREAAAGAGEDALLKLMDATCRRA 462

Query: 421 TAPPAPPDGLSLVDVGYTEFDS 435
           TAPPAPPDGLSLVD    EF S
Sbjct: 463 TAPPAPPDGLSLVDKVGWEFPS 483

[Close]

BLAST of Sed0005150 vs. ExPASy TrEMBL
Match: A0A6J1L1X8 (tRNA pseudouridine synthase OS=Cucurbita maxima OX=3661 GN=LOC111499102 PE=3 SV=1)

HSP 1 Score: 736.1 bits (1899), Expect = 8.6e-209
Identity = 370/434 (85.25%), Postives = 391/434 (90.09%), Query Frame = 0


Query: 1   MIPNSRARILSSSGASFGF------KALMENQAPKKDYLHYNHTDRCRYARWTSRESYQL 60
           M+PN RAR+LS SGA FGF      K LMEN+A KKDYLHYNHTDRCR ARWTSRESYQL
Sbjct: 43  MLPNPRARLLSPSGAGFGFGFGFGIKCLMENRAQKKDYLHYNHTDRCRNARWTSRESYQL 102

Query: 61  MYGRQWQEVLDFYSHAVNGRFSLSSLFGTETVHHDDAAEIQPICNPAELSRASVKDRTGR 120
           MY R WQEVLDFYSHAVNGR SLSSL  TETV   D  EIQP+CN AEL+RA VKD TGR
Sbjct: 103 MYERPWQEVLDFYSHAVNGRLSLSSL-RTETVRDHDDTEIQPVCNTAELNRAPVKDGTGR 162

Query: 121 WARTTFKIVLSYHGNSFDGWQKQPGLDTVQGVVERSLGQFVDEKKAQQLKDRCLPLEGCA 180
           WAR TFKIVLSYHGNSFDGWQKQP L+TVQGVVE+SLG+FVDEKKAQQLKDRCLP+EGCA
Sbjct: 163 WARATFKIVLSYHGNSFDGWQKQPELNTVQGVVEKSLGKFVDEKKAQQLKDRCLPIEGCA 222

Query: 181 AVAGRTDKGVTAMRQVCAFYTWRTDVKPQDIEDSINNAAPGKLRVLSVSQVSRMFHPNFS 240
            VAGRTDKGVTA+RQVC+FYTWRTDVKPQDIE SINNAAPGKLRVLSVSQVSRMFHPNFS
Sbjct: 223 VVAGRTDKGVTALRQVCSFYTWRTDVKPQDIEGSINNAAPGKLRVLSVSQVSRMFHPNFS 282

Query: 241 AKWRRYLYIFPLNDRENMEPDFAILE--EKCREDNSCDEIEVANGECKDEVLDNLVLSRV 300
           AKWRRYLYIFPL D ENMEP+FAILE  E  RE+   D+IEV +GECKDE+L+NL+LS+ 
Sbjct: 283 AKWRRYLYIFPLKDGENMEPNFAILEDVENSREEEYRDKIEVLHGECKDEILNNLILSQD 342

Query: 301 EEEPFSTDKPQTFSIRRVNQLLRKLEGKLLSYKMFARDTKASRNEGPPTECFVYHARAME 360
           +EEP S +KPQTFSI RVNQLLRKLEGKLLSYKMFARDTKASRNEGPPTECFVYHARAME
Sbjct: 343 DEEPASANKPQTFSIMRVNQLLRKLEGKLLSYKMFARDTKASRNEGPPTECFVYHARAME 402

Query: 361 ARLPCLDQGVGRSVMCVELVANRFLRKMVRVLVATAIREAAAGAGEDALLKLMDATCRRA 420
           ARLPCLDQGVGRSVMCVELVANRFLRKMVRVLVATAIREAAAGAGEDALLKLMDATCRRA
Sbjct: 403 ARLPCLDQGVGRSVMCVELVANRFLRKMVRVLVATAIREAAAGAGEDALLKLMDATCRRA 462

Query: 421 TAPPAPPDGLSLVD 427
           TAPPAPPDGLSLVD
Sbjct: 463 TAPPAPPDGLSLVD 475

[Close]

BLAST of Sed0005150 vs. TAIR 10
Match: AT5G35400.1 (Pseudouridine synthase family protein )

HSP 1 Score: 479.6 bits (1233), Expect = 2.8e-135
Identity = 244/417 (58.51%), Postives = 301/417 (72.18%), Query Frame = 0


Query: 32  YLHYNHTDRCRYARWTSRESYQLMYGRQWQEVLDFYSHAVNGRFSLSSLF-GTETVHHDD 91
           Y+HY+H D C  ARW +RE+++ MY R WQ VLDFYS+AV G  S+ SLF   +++ HDD
Sbjct: 6   YVHYHHADACSKARWNARETFRFMYDRPWQHVLDFYSNAVEGNLSVLSLFQPKKSLVHDD 65

Query: 92  AAEIQPICNPAELSRASVKDRTGRWARTTFKIVLSYHGNSFDGWQKQPGLDTVQGVVERS 151
               + +      +      R+GRW R  FKIVLSY+G SFDGWQKQP L TVQGVVE+S
Sbjct: 66  GITEEMLLESEPETSTRKDGRSGRWERVNFKIVLSYNGASFDGWQKQPDLHTVQGVVEKS 125

Query: 152 LGQFVDEKKAQQLKDRCLPLEGCAAVAGRTDKGVTAMRQVCAFYTWRTDVKPQDIEDSIN 211
           LG FVDE+KAQ LK +C PLEG   VAGRTDKGV+A+ QVC+FYTWR D++P  IED+IN
Sbjct: 126 LGGFVDERKAQLLKKKCKPLEGRVLVAGRTDKGVSALNQVCSFYTWRKDIEPIAIEDAIN 185

Query: 212 NAAPGKLRVLSVSQVSRMFHPNFSAKWRRYLYIFPLNDRENMEPDFAILEEKCREDNSCD 271
             A GKLRV+S+S+VSR FHPNFSAKWRRYLYIFPL+D E +       E    ++N   
Sbjct: 186 KDASGKLRVVSISKVSRSFHPNFSAKWRRYLYIFPLDDAEPLRKSGENHENFIFDENKKK 245

Query: 272 EIEVANGECKDEVLDNLVLSRVEEEPFS--------TDKPQTFSIRRVNQLLRKLEGKLL 331
           +    NG   +E+ + +++S  +E             +KP  FS+ +V+QLL+ L+GK+L
Sbjct: 246 Q---RNGLLSEEIAEEVIMSEDDELEIEETSNALEVVEKPSDFSVSKVDQLLQHLQGKVL 305

Query: 332 SYKMFARDTKASRNEGPPTECFVYHARAMEARLPCLDQGVGRSVMCVELVANRFLRKMVR 391
           SYKMFARDTKA+RNEGPPTECF+YHARA E RL   D   GR VMCVELVANRFLRKMVR
Sbjct: 306 SYKMFARDTKAARNEGPPTECFMYHARAAEIRLSSSDCVEGRRVMCVELVANRFLRKMVR 365

Query: 392 VLVATAIREAAAGAGEDALLKLMDATCRRATAPPAPPDGLSLVDVGYTEFDSRNCFI 440
           VLVAT+IREAAAGA +DALLKL++A+CRRATAPPAP +GL L DVGYT+FD +   I
Sbjct: 366 VLVATSIREAAAGAEDDALLKLLEASCRRATAPPAPSEGLCLFDVGYTDFDPQTSLI 419

[Close]

BLAST of Sed0005150 vs. TAIR 10
Match: AT5G35400.2 (Pseudouridine synthase family protein )

HSP 1 Score: 456.1 bits (1172), Expect = 3.3e-128
Identity = 244/468 (52.14%), Postives = 301/468 (64.32%), Query Frame = 0


Query: 32  YLHYNHTDRCRYARWTSRESYQLMYGRQWQEVLDFYSHAVNGRFSLSSLF-GTETVHHDD 91
           Y+HY+H D C  ARW +RE+++ MY R WQ VLDFYS+AV G  S+ SLF   +++ HDD
Sbjct: 6   YVHYHHADACSKARWNARETFRFMYDRPWQHVLDFYSNAVEGNLSVLSLFQPKKSLVHDD 65

Query: 92  AAEIQPICNPAELSRASVKDRTGRWARTTFKIVLSYHGNSFDGWQKQPGLDTVQGVVERS 151
               + +      +      R+GRW R  FKIVLSY+G SFDGWQKQP L TVQGVVE+S
Sbjct: 66  GITEEMLLESEPETSTRKDGRSGRWERVNFKIVLSYNGASFDGWQKQPDLHTVQGVVEKS 125

Query: 152 LGQFVDEKKAQQLKDRCLPLEGCAAVAGRTDKGVTAMRQVCAFYTWRTDVKPQDIEDSIN 211
           LG FVDE+KAQ LK +C PLEG   VAGRTDKGV+A+ QVC+FYTWR D++P  IED+IN
Sbjct: 126 LGGFVDERKAQLLKKKCKPLEGRVLVAGRTDKGVSALNQVCSFYTWRKDIEPIAIEDAIN 185

Query: 212 NAAPGKLRVLSVSQVSRMFHPNFSAKWRRYLYIFPLNDRENMEPDFAILEEKCREDNSCD 271
             A GKLRV+S+S+VSR FHPNFSAKWRRYLYIFPL+D E +       E    ++N   
Sbjct: 186 KDASGKLRVVSISKVSRSFHPNFSAKWRRYLYIFPLDDAEPLRKSGENHENFIFDENKKK 245

Query: 272 EIEVANGECKDEVLDNLVLSRVEEEPFS--------TDKPQTFSIRRVNQLLRKLEGKLL 331
           +    NG   +E+ + +++S  +E             +KP  FS+ +V+QLL+ L+GK+L
Sbjct: 246 Q---RNGLLSEEIAEEVIMSEDDELEIEETSNALEVVEKPSDFSVSKVDQLLQHLQGKVL 305

Query: 332 SYKMFARDTKASRNEGPPTECFVYHARAMEARLP-------------------------- 391
           SYKMFARDTKA+RNEGPPTECF+YHARA E RL                           
Sbjct: 306 SYKMFARDTKAARNEGPPTECFMYHARAAEIRLSSSVVHFVAWVFGGSIVLVILATEGST 365

Query: 392 -------------------------CLDQGVGRSVMCVELVANRFLRKMVRVLVATAIRE 440
                                      D   GR VMCVELVANRFLRKMVRVLVAT+IRE
Sbjct: 366 LDLTDTKLYIASWFLIKRSCEFLFIASDCVEGRRVMCVELVANRFLRKMVRVLVATSIRE 425

[Close]

BLAST of Sed0005150 vs. TAIR 10
Match: AT3G06950.1 (Pseudouridine synthase family protein )

HSP 1 Score: 67.8 bits (164), Expect = 2.5e-11
Identity = 77/316 (24.37%), Postives = 120/316 (37.97%), Query Frame = 0


Query: 120 FKIVLSYHGNSFDGWQKQPGLDTVQGVVERSLGQFVDEKKAQQLKDRCLPLEGCAAVAGR 179
           +++V++Y G  F GWQ Q    T+Q ++E++L Q        +L  + L L G    AGR
Sbjct: 43  WRLVIAYDGTRFAGWQYQESPPTIQSMLEKALIQIT------ELGRKELQLIG----AGR 102

Query: 180 TDKGVTAMRQVCAFYTWRTDVKPQDIEDSINNAAPGKLRVLSVSQVSRMFHPNFSAKWRR 239
           TD GV A  QV  F T            ++N   P  +RV  +S     FH  FSA  + 
Sbjct: 103 TDAGVHAWGQVAHFVTPFNYTSLDSFHAALNGLLPKDIRVRELSAAVPEFHARFSASSKV 162

Query: 240 YLYIFPLNDRENMEPDFAILEEKCREDNSCDEIEVANGECKDEVLDNLVLSRVEEEPFST 299
           Y Y                              ++ N    D  +D          PF  
Sbjct: 163 YRY------------------------------QIYN----DTFMD----------PFQR 222

Query: 300 DKPQTFSIRRVNQLLRKLEGKLLSYKMFARDTKASRNEGPPTECFVYHARAMEARLPCLD 359
                 + +     +R+     +    F+    A+R +G P            +R   + 
Sbjct: 223 HWAYHCAYKLNASKMREAANLFVGKHDFSAFANATREDGVPDPLKTI------SRFDVIQ 282

Query: 360 QGVGRSVMCVELVANRFLRKMVRVLVATAIREAAAGAGEDALLKLMDATCRRA----TAP 419
            G   S++ +E+  + FL + VR +VA  I+        D +  +++   RR     T+ 
Sbjct: 283 MG---SLLQLEVEGSGFLYRQVRNMVALLIQIGKEALDSDIVPMILETKDRRVLAKYTSL 295

Query: 420 PAPPDGLSLVDVGYTE 432
           PA P GL LV V Y E
Sbjct: 343 PASPHGLCLVSVKYKE 295

[Close]

BLAST of Sed0005150 vs. TAIR 10
Match: AT1G20370.1 (Pseudouridine synthase family protein )

HSP 1 Score: 63.5 bits (153), Expect = 4.8e-10
Identity = 42/136 (30.88%), Postives = 65/136 (47.79%), Query Frame = 0


Query: 111 RTGRWARTTFKIVLSYHGNSFDGWQKQPGLDTVQGVVERSLGQFVDEKKAQQLKDRCLPL 170
           R  ++ R    IV ++ G  + G QK PG  T++G +E +L        A    DR  P 
Sbjct: 51  RAPKYRRRKVAIVFAFCGVGYQGMQKNPGAKTIEGELEEAL----FHAGAVPDADRNKPR 110

Query: 171 EGCAAVAGRTDKGVTAMRQVCAFYTWRTDVKPQDIEDSINNAAPGKLRVLSVSQVSRMFH 230
               A + RTDKGV+A+ QV    + R  V P    + +N+  P ++RV    +V+  F 
Sbjct: 111 NYEWARSARTDKGVSAVGQV---VSGRFYVDPPGFVERLNSKLPDQIRVFGYKRVAPSFS 170

Query: 231 PNFSAKWRRYLYIFPL 247
                  RRY+Y+ P+
Sbjct: 171 SKKFCDRRRYVYLIPV 179

[Close]

BLAST of Sed0005150 vs. TAIR 10
Match: AT1G76120.1 (Pseudouridine synthase family protein )

HSP 1 Score: 57.4 bits (137), Expect = 3.4e-08
Identity = 43/154 (27.92%), Postives = 70/154 (45.45%), Query Frame = 0


Query: 122 IVLSYHGNSFDGWQKQPGLDTVQGVVERSLGQFVDEKKAQQLKDRCLPLEGCAAVAGRTD 181
           I+ ++ G  + G QK PG  T++G +E +L       ++ + K +        A + RTD
Sbjct: 26  IIFAFCGVGYQGMQKNPGAKTIEGELEEALFHAGAVPESIRGKPKLYDF----ARSARTD 85

Query: 182 KGVTAMRQVCAFYTWRTDVKPQDIEDSINNAAPGKLRVLSVSQVSRMFHPNFSAKWRRYL 241
           KGV+A+ QV    + R  V P    + +N+  P ++R+     V+  F        RRY+
Sbjct: 86  KGVSAVGQV---VSGRFIVDPLGFVNRLNSNLPNQIRIFGYKHVTPSFSSKKFCDRRRYV 145

Query: 242 YIFPL--------NDRENMEPDFAILEE--KCRE 266
           Y+ P+         DRE +       EE  KC E
Sbjct: 146 YLLPVFALDPISHRDRETVMASLGPGEEYVKCFE 172

The following BLAST results are available for this feature:

BLAST of Sed0005150 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023006334.1	4.2e-218	85.33	uncharacterized protein LOC111499102 isoform X1 [Cucurbita maxima]	[more]
XP_022933289.1	3.6e-217	85.87	uncharacterized protein LOC111440432 isoform X1 [Cucurbita moschata]	[more]
XP_023531012.1	7.9e-217	86.10	uncharacterized protein LOC111793396 isoform X1 [Cucurbita pepo subsp. pepo]	[more]
XP_038878348.1	1.4e-216	84.49	tRNA pseudouridine synthase A [Benincasa hispida]	[more]
KAG7021669.1	1.4e-216	85.65	truA, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]

BLAST of Sed0005150 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q01WL4	2.2e-12	26.21	tRNA pseudouridine synthase A OS=Solibacter usitatus (strain Ellin6076) OX=23426...	[more]
B1LB83	2.2e-12	33.85	tRNA pseudouridine synthase A OS=Thermotoga sp. (strain RQ2) OX=126740 GN=truA P...	[more]
Q73KE3	2.2e-12	24.53	tRNA pseudouridine synthase A OS=Treponema denticola (strain ATCC 35405 / DSM 14...	[more]
B9K8Q0	6.5e-12	23.67	tRNA pseudouridine synthase A OS=Thermotoga neapolitana (strain ATCC 49049 / DSM...	[more]
B7K222	1.1e-11	33.59	tRNA pseudouridine synthase A OS=Rippkaea orientalis (strain PCC 8801) OX=41431 ...	[more]

BLAST of Sed0005150 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1L1W3	2.0e-218	85.33	tRNA pseudouridine synthase OS=Cucurbita maxima OX=3661 GN=LOC111499102 PE=3 SV=...	[more]
A0A6J1EZC2	1.7e-217	85.87	tRNA pseudouridine synthase OS=Cucurbita moschata OX=3662 GN=LOC111440432 PE=3 S...	[more]
A0A6J1C0W1	3.1e-211	83.86	tRNA pseudouridine synthase OS=Momordica charantia OX=3673 GN=LOC111007251 PE=3 ...	[more]
A0A6J1L1X2	2.3e-209	84.39	tRNA pseudouridine synthase OS=Cucurbita maxima OX=3661 GN=LOC111499102 PE=3 SV=...	[more]
A0A6J1L1X8	8.6e-209	85.25	tRNA pseudouridine synthase OS=Cucurbita maxima OX=3661 GN=LOC111499102 PE=3 SV=...	[more]

BLAST of Sed0005150 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G35400.1	2.8e-135	58.51	Pseudouridine synthase family protein	[more]
AT5G35400.2	3.3e-128	52.14	Pseudouridine synthase family protein	[more]
AT3G06950.1	2.5e-11	24.37	Pseudouridine synthase family protein	[more]
AT1G20370.1	4.8e-10	30.88	Pseudouridine synthase family protein	[more]
AT1G76120.1	3.4e-08	27.92	Pseudouridine synthase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Chayote (edule) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR020097	Pseudouridine synthase I, TruA, alpha/beta domain	PFAM	PF01416	PseudoU_synth_1	coord: 341..429 e-value: 1.5E-6 score: 28.6
IPR020094	Pseudouridine synthase TruA/RsuA/RluB/E/F, N-terminal	GENE3D	3.30.70.580		coord: 116..235 e-value: 1.6E-24 score: 88.2
IPR020095	Pseudouridine synthase I, TruA, C-terminal	GENE3D	3.30.70.660		coord: 283..427 e-value: 8.3E-15 score: 56.8
None	No IPR available	PANTHER	PTHR11142:SF10	TRNA PSEUDOURIDINE SYNTHASE	coord: 40..439
IPR001406	Pseudouridine synthase I, TruA	PANTHER	PTHR11142	PSEUDOURIDYLATE SYNTHASE	coord: 40..439
IPR020103	Pseudouridine synthase, catalytic domain superfamily	SUPERFAMILY	55120	Pseudouridine synthase	coord: 118..435

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Sed0005150.1	Sed0005150.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0031119	tRNA pseudouridine synthesis
biological_process	GO:0001522	pseudouridine synthesis
biological_process	GO:0009451	RNA modification
molecular_function	GO:0003723	RNA binding
molecular_function	GO:0106029	tRNA pseudouridine synthase activity
molecular_function	GO:0009982	pseudouridine synthase activity