Sed0002234 (gene) Chayote v1

Overview
NameSed0002234
Typegene
OrganismSechium edule (Chayote v1)
Descriptionnon-specific phospholipase C6-like
LocationLG08: 97027 .. 99331 (+)
RNA-Seq ExpressionSed0002234
SyntenySed0002234
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CAAGACCAGAAAAAACTTCATCATCATCTTCTTCCTCTTCTTCTCTCTCTATCACAGTCATTTATAAGTGGCATTTGTTTGTAACAATCTCATCCACAACGCTCTCTTACATTTCCACACCATCAATGGTAGGATTTGGCTTCTTTTTGTTGATCTTTCTGAGTCTTTCATGGCTTCCTCAACCCAGTTTCCAGCAGCAACCCATCCAAACCGTCGTCGTTTTGGTGTTGGAGAATCGCTCATTCGATCACATGCTTGGATGGATGAAGAAATACATGGACGACTCTCGAATCAACGGCGTCACCGGCCACGAATGCAACCCTGTTTCCACCAACAATCCAAACCCTCAAACCGTTTGCTTCTCGGACGACGCCGAGTTTGTAGATCCCGATCCTGGCCACTCCTTCGAAGCCGTTCTACACCAGGTCTTTGGCTCTGGCTCCATTCCCTCCATGTCTGGCTTTGTGGAGCAGGCTCTTTCAATCTCACCCAACCTCTCTCAGGCTGTCATGAAAGGCTTCAAACCTCAATCCCTGCCTGTTTATGCTGCTCTTGTTCCTGAATTTGCAGTCTTTGATAGGTGGTTCTCTTCAATTCCCGGCCCAACTCAACCCAATAGGCTCTTTGTTTACTCTGCAACTTCCCACGGTTCAACCAGCCACCTGAAGAAGCAATTAGCAGTGGGGTATCCACAGAAAACCATTTTCGATTCGCTCCACGAGAATGGTCTCGACTTCGGAATCTACTTCCAAAACATACCCACCACTTTGTTTTACAGAAACTTGAGGAAACTGAAGTACATTTTCAAGTTTCACGACTACGATTTGAAGTTCAAGAAGGATGCTACAAATGGGAAGCTCCCGAGCTTAACAGTGATTGAGCCACGCTATTTCGATCTCGTGGGAATGCCTGCAAATGATGACCATCCGTCTCATGATGTTGCCAATGGCCAAAAGCTCGTGAAAGAGGTTTACGAGACTCTGAGGGCTAGCCCACAGTGGAACCAGACACTTCTGATAATCACTTATGATGAGCATGGTGGGTTTTTTGACCATGTCAACACTCCTTTTGTCAATGTCCCTAATCCTGATGGAAACACAGGCCCTGCTCCTTATTTCTTCAAGTTCGATCGCCTTGGAGTCCGTGTGCCTACAATCATGGTATCCCCTTGGATTAAGAAAGGGACTAGTAAGCCAAGTTCTTTCTCGTGACTTACTTTCAGGTTGTCTGTTCCGTGAGAATAATCTAGGTGCATGTAAAAAAAAAAAGAGAAAGAAATGGAAGGCTTTAGTTGTTACAAATGGCCTAAAACTTAATTTATGTGGTTGGTGGGAACCCTCATGCTTCCTCTTGTGGAACAGTTGGTTGGATTTGGGATCCACTCCAGTTGGTTAAATTTAGTGTCATTCTATCAAAACTAGCATCATCTTCACTTCCATATTGTCTCTGGCGTTTTAAAGCACTGGGGTCCTGATCTAATTAAACCAGTTTGAAATAACTTGGATGGTTAGTCATCATTCCAAACCAGTGCTTCAATTAGTATGAACTTAACCGATACAAAGACAAGCGCGGAGACCATTGTCCTAAATCAAATCCAGAGTTTGATTTGAATGTGTTTGTTTTGCAGTAATAAGCAGTCCAAAGGGTCCTACTCCAAACTCTGAGTTTGAACTTTCCTCAATCCCTGCTACCATAAAGAAAATATTCAATCTCTCCTCCAACTTCCTGACTCACAGAGATGCATGGGCTGGGACATTTGAGGACATTGTTGGGCAACTAACCTCGCCAAGAACCGATTGTCCAGGTATCTATCTACCTATCTATCTATCTGTACTGATTGTAACACATCAAAGGTGTAAATGAAAGTTGTTAATTTGGCTACCATTTGTTGTTGTAGTAACCTTGCCTGAAGTGACAGCTTTGAGGAAAACAGAAGCAAACGAAAACAGTGCAATATCTGAGTTTCAGAGTGAAGTAGTTCAGTTAGGTGCAGTTCTGAATGGTGACCATTTCCTTAGTAGCTTCCCTGATGAAATAAGCAAGAAGATGAGCGTAAAAGAAGCACATGAGTACACGAAAGGAGCTGTTTCAAGGTTCATCAGAGCAAGCAAAGAGGCTATCAAGTTGGGTGCAGATGAGTCTGCCATTGTGGATATGAGATCTTCCCTTACCACAAGATCCTCAATGCACAAATGAATGCATACATTATTCTTCCACACTATAAAACCTACTACATTTTAGAATTTGACAGCAATTCTAGTCTGTAACTATGTTTTCATATATATATATATATATATATATATAT

mRNA sequence

CAAGACCAGAAAAAACTTCATCATCATCTTCTTCCTCTTCTTCTCTCTCTATCACAGTCATTTATAAGTGGCATTTGTTTGTAACAATCTCATCCACAACGCTCTCTTACATTTCCACACCATCAATGGTAGGATTTGGCTTCTTTTTGTTGATCTTTCTGAGTCTTTCATGGCTTCCTCAACCCAGTTTCCAGCAGCAACCCATCCAAACCGTCGTCGTTTTGGTGTTGGAGAATCGCTCATTCGATCACATGCTTGGATGGATGAAGAAATACATGGACGACTCTCGAATCAACGGCGTCACCGGCCACGAATGCAACCCTGTTTCCACCAACAATCCAAACCCTCAAACCGTTTGCTTCTCGGACGACGCCGAGTTTGTAGATCCCGATCCTGGCCACTCCTTCGAAGCCGTTCTACACCAGGTCTTTGGCTCTGGCTCCATTCCCTCCATGTCTGGCTTTGTGGAGCAGGCTCTTTCAATCTCACCCAACCTCTCTCAGGCTGTCATGAAAGGCTTCAAACCTCAATCCCTGCCTGTTTATGCTGCTCTTGTTCCTGAATTTGCAGTCTTTGATAGGTGGTTCTCTTCAATTCCCGGCCCAACTCAACCCAATAGGCTCTTTGTTTACTCTGCAACTTCCCACGGTTCAACCAGCCACCTGAAGAAGCAATTAGCAGTGGGGTATCCACAGAAAACCATTTTCGATTCGCTCCACGAGAATGGTCTCGACTTCGGAATCTACTTCCAAAACATACCCACCACTTTGTTTTACAGAAACTTGAGGAAACTGAAGTACATTTTCAAGTTTCACGACTACGATTTGAAGTTCAAGAAGGATGCTACAAATGGGAAGCTCCCGAGCTTAACAGTGATTGAGCCACGCTATTTCGATCTCGTGGGAATGCCTGCAAATGATGACCATCCGTCTCATGATGTTGCCAATGGCCAAAAGCTCGTGAAAGAGGTTTACGAGACTCTGAGGGCTAGCCCACAGTGGAACCAGACACTTCTGATAATCACTTATGATGAGCATGTAATAAGCAGTCCAAAGGGTCCTACTCCAAACTCTGAGTTTGAACTTTCCTCAATCCCTGCTACCATAAAGAAAATATTCAATCTCTCCTCCAACTTCCTGACTCACAGAGATGCATGGGCTGGGACATTTGAGGACATTGTTGGGCAACTAACCTCGCCAAGAACCGATTGTCCAGTAACCTTGCCTGAAGTGACAGCTTTGAGGAAAACAGAAGCAAACGAAAACAGTGCAATATCTGAGTTTCAGAGTGAAGTAGTTCAGTTAGGTGCAGTTCTGAATGGTGACCATTTCCTTAGTAGCTTCCCTGATGAAATAAGCAAGAAGATGAGCGTAAAAGAAGCACATGAGTACACGAAAGGAGCTGTTTCAAGGTTCATCAGAGCAAGCAAAGAGGCTATCAAGTTGGGTGCAGATGAGTCTGCCATTGTGGATATGAGATCTTCCCTTACCACAAGATCCTCAATGCACAAATGAATGCATACATTATTCTTCCACACTATAAAACCTACTACATTTTAGAATTTGACAGCAATTCTAGTCTGTAACTATGTTTTCATATATATATATATATATATATATATAT

Coding sequence (CDS)

ATGGTAGGATTTGGCTTCTTTTTGTTGATCTTTCTGAGTCTTTCATGGCTTCCTCAACCCAGTTTCCAGCAGCAACCCATCCAAACCGTCGTCGTTTTGGTGTTGGAGAATCGCTCATTCGATCACATGCTTGGATGGATGAAGAAATACATGGACGACTCTCGAATCAACGGCGTCACCGGCCACGAATGCAACCCTGTTTCCACCAACAATCCAAACCCTCAAACCGTTTGCTTCTCGGACGACGCCGAGTTTGTAGATCCCGATCCTGGCCACTCCTTCGAAGCCGTTCTACACCAGGTCTTTGGCTCTGGCTCCATTCCCTCCATGTCTGGCTTTGTGGAGCAGGCTCTTTCAATCTCACCCAACCTCTCTCAGGCTGTCATGAAAGGCTTCAAACCTCAATCCCTGCCTGTTTATGCTGCTCTTGTTCCTGAATTTGCAGTCTTTGATAGGTGGTTCTCTTCAATTCCCGGCCCAACTCAACCCAATAGGCTCTTTGTTTACTCTGCAACTTCCCACGGTTCAACCAGCCACCTGAAGAAGCAATTAGCAGTGGGGTATCCACAGAAAACCATTTTCGATTCGCTCCACGAGAATGGTCTCGACTTCGGAATCTACTTCCAAAACATACCCACCACTTTGTTTTACAGAAACTTGAGGAAACTGAAGTACATTTTCAAGTTTCACGACTACGATTTGAAGTTCAAGAAGGATGCTACAAATGGGAAGCTCCCGAGCTTAACAGTGATTGAGCCACGCTATTTCGATCTCGTGGGAATGCCTGCAAATGATGACCATCCGTCTCATGATGTTGCCAATGGCCAAAAGCTCGTGAAAGAGGTTTACGAGACTCTGAGGGCTAGCCCACAGTGGAACCAGACACTTCTGATAATCACTTATGATGAGCATGTAATAAGCAGTCCAAAGGGTCCTACTCCAAACTCTGAGTTTGAACTTTCCTCAATCCCTGCTACCATAAAGAAAATATTCAATCTCTCCTCCAACTTCCTGACTCACAGAGATGCATGGGCTGGGACATTTGAGGACATTGTTGGGCAACTAACCTCGCCAAGAACCGATTGTCCAGTAACCTTGCCTGAAGTGACAGCTTTGAGGAAAACAGAAGCAAACGAAAACAGTGCAATATCTGAGTTTCAGAGTGAAGTAGTTCAGTTAGGTGCAGTTCTGAATGGTGACCATTTCCTTAGTAGCTTCCCTGATGAAATAAGCAAGAAGATGAGCGTAAAAGAAGCACATGAGTACACGAAAGGAGCTGTTTCAAGGTTCATCAGAGCAAGCAAAGAGGCTATCAAGTTGGGTGCAGATGAGTCTGCCATTGTGGATATGAGATCTTCCCTTACCACAAGATCCTCAATGCACAAATGA

Protein sequence

MVGFGFFLLIFLSLSWLPQPSFQQQPIQTVVVLVLENRSFDHMLGWMKKYMDDSRINGVTGHECNPVSTNNPNPQTVCFSDDAEFVDPDPGHSFEAVLHQVFGSGSIPSMSGFVEQALSISPNLSQAVMKGFKPQSLPVYAALVPEFAVFDRWFSSIPGPTQPNRLFVYSATSHGSTSHLKKQLAVGYPQKTIFDSLHENGLDFGIYFQNIPTTLFYRNLRKLKYIFKFHDYDLKFKKDATNGKLPSLTVIEPRYFDLVGMPANDDHPSHDVANGQKLVKEVYETLRASPQWNQTLLIITYDEHVISSPKGPTPNSEFELSSIPATIKKIFNLSSNFLTHRDAWAGTFEDIVGQLTSPRTDCPVTLPEVTALRKTEANENSAISEFQSEVVQLGAVLNGDHFLSSFPDEISKKMSVKEAHEYTKGAVSRFIRASKEAIKLGADESAIVDMRSSLTTRSSMHK
Homology
BLAST of Sed0002234 vs. NCBI nr
Match: XP_022921419.1 (non-specific phospholipase C6-like [Cucurbita moschata])

HSP 1 Score: 812.4 bits (2097), Expect = 2.0e-231
Identity = 412/508 (81.10%), Postives = 437/508 (86.02%), Query Frame = 0

Query: 4   FGFFLLIFLSLSWLPQPSFQQQPIQTVVVLVLENRSFDHMLGWMKKYMDDSRINGVTGHE 63
           F FFLL  L+LSW+PQ +FQQQPI+TVVVLV+ENRSFDHM+GWMKKY+ D RINGVTG E
Sbjct: 14  FIFFLL--LTLSWIPQRTFQQQPIKTVVVLVMENRSFDHMIGWMKKYI-DPRINGVTGDE 73

Query: 64  CNPVSTNNPNPQTVCFSDDAEFVDPDPGHSFEAVLHQVFGSGSIPSMSGFVEQALSISPN 123
           CNPVST NPNP+T+CFSDDAEFVDPDPGHSFE VL QVFGS SIPSMSGFVEQALS+S N
Sbjct: 74  CNPVSTKNPNPETICFSDDAEFVDPDPGHSFEDVLQQVFGSNSIPSMSGFVEQALSMSSN 133

Query: 124 LSQAVMKGFKPQSLPVYAALVPEFAVFDRWFSSIPGPTQPNRLFVYSATSHGSTSHLKKQ 183
           LS+ VMKGFKP+++P+Y ALV EFAVFDRWFSSIPGPTQPNRLFVYSATSHGSTSH+KKQ
Sbjct: 134 LSETVMKGFKPEAVPIYGALVREFAVFDRWFSSIPGPTQPNRLFVYSATSHGSTSHVKKQ 193

Query: 184 LAVGYPQKTIFDSLHENGLDFGIYFQNIPTTLFYRNLRKLKYIFKFHDYDLKFKKDATNG 243
           LA+GYPQKTIFDSLHENGLDFG+YFQNIPTTLFYRNLRKLKYIFKFH YDL+FKKDA NG
Sbjct: 194 LALGYPQKTIFDSLHENGLDFGVYFQNIPTTLFYRNLRKLKYIFKFHPYDLQFKKDARNG 253

Query: 244 KLPSLTVIEPRYFDLVGMPANDDHPSHDVANGQKLVKEVYETLRASPQWNQTLLIITYDE 303
           KLPSLTVIEPRYFDLVGMPANDDHPSHDVANGQKLVKEVYETLRASPQWNQTLLIITYDE
Sbjct: 254 KLPSLTVIEPRYFDLVGMPANDDHPSHDVANGQKLVKEVYETLRASPQWNQTLLIITYDE 313

Query: 304 H--------------------------------------------------VISSPKGPT 363
           H                                                  VISSPKGPT
Sbjct: 314 HGGFFDHVRTPFVNVPNPDGNTGPAPYFFKFDRLGVRVPTIMVSPWIKKGTVISSPKGPT 373

Query: 364 PNSEFELSSIPATIKKIFNLSSNFLTHRDAWAGTFEDIVGQLTSPRTDCPVTLPEVTALR 423
           PNSEFE SSIPATIKKIFNLSSNFLTHRDAWAGTFEDIVGQLTSPRTDCPVTLPEVT LR
Sbjct: 374 PNSEFEHSSIPATIKKIFNLSSNFLTHRDAWAGTFEDIVGQLTSPRTDCPVTLPEVTPLR 433

Query: 424 KTEANENSAISEFQSEVVQLGAVLNGDHFLSSFPDEISKKMSVKEAHEYTKGAVSRFIRA 462
           KTEANENS +SEFQSEVVQL AVLNGDHFLSSFP+EIS+KM+VKEAH+YT+GAVSRFIRA
Sbjct: 434 KTEANENSGVSEFQSEVVQLAAVLNGDHFLSSFPNEISEKMTVKEAHDYTRGAVSRFIRA 493

BLAST of Sed0002234 vs. NCBI nr
Match: XP_023515458.1 (non-specific phospholipase C6-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 811.6 bits (2095), Expect = 3.5e-231
Identity = 412/508 (81.10%), Postives = 437/508 (86.02%), Query Frame = 0

Query: 4   FGFFLLIFLSLSWLPQPSFQQQPIQTVVVLVLENRSFDHMLGWMKKYMDDSRINGVTGHE 63
           F FFLL  L+LSW+PQ +FQQQPI+TVVVLV+ENRSFDHM+GWMKKY+ D RINGVTG E
Sbjct: 14  FIFFLL--LTLSWIPQRTFQQQPIKTVVVLVMENRSFDHMIGWMKKYI-DPRINGVTGDE 73

Query: 64  CNPVSTNNPNPQTVCFSDDAEFVDPDPGHSFEAVLHQVFGSGSIPSMSGFVEQALSISPN 123
           CNPVST NPNP+T+CFSDDAEFVDPDPGHSFE VL QVFGS SIPSMSGFVEQALS+S N
Sbjct: 74  CNPVSTKNPNPETICFSDDAEFVDPDPGHSFEDVLQQVFGSNSIPSMSGFVEQALSMSSN 133

Query: 124 LSQAVMKGFKPQSLPVYAALVPEFAVFDRWFSSIPGPTQPNRLFVYSATSHGSTSHLKKQ 183
           LS+ VMKGFKP+++P+Y ALV EFAVFDRWFSSIPGPTQPNRLFVYSATSHGSTSH+KKQ
Sbjct: 134 LSETVMKGFKPEAVPIYGALVREFAVFDRWFSSIPGPTQPNRLFVYSATSHGSTSHVKKQ 193

Query: 184 LAVGYPQKTIFDSLHENGLDFGIYFQNIPTTLFYRNLRKLKYIFKFHDYDLKFKKDATNG 243
           LA+GYPQKTIFDSLHENGLDFG+YFQNIPTTLFYRNLRKLKYIFKFH YDL+FKKDA NG
Sbjct: 194 LALGYPQKTIFDSLHENGLDFGVYFQNIPTTLFYRNLRKLKYIFKFHPYDLQFKKDARNG 253

Query: 244 KLPSLTVIEPRYFDLVGMPANDDHPSHDVANGQKLVKEVYETLRASPQWNQTLLIITYDE 303
           KLPSLTVIEPRYFDLVGMPANDDHPSHDVANGQKLVKEVYETLRASPQWNQTLLIITYDE
Sbjct: 254 KLPSLTVIEPRYFDLVGMPANDDHPSHDVANGQKLVKEVYETLRASPQWNQTLLIITYDE 313

Query: 304 H--------------------------------------------------VISSPKGPT 363
           H                                                  VISSPKGPT
Sbjct: 314 HGGFFDHVKTPFVNVPNPDGNTGPAPYFFKFDRLGVRVPTIMVSPWIKKGTVISSPKGPT 373

Query: 364 PNSEFELSSIPATIKKIFNLSSNFLTHRDAWAGTFEDIVGQLTSPRTDCPVTLPEVTALR 423
           PNSEFE SSIPATIKKIFNLSSNFLTHRDAWAGTFEDIVGQLTSPRTDCPVTLPEVT LR
Sbjct: 374 PNSEFEHSSIPATIKKIFNLSSNFLTHRDAWAGTFEDIVGQLTSPRTDCPVTLPEVTPLR 433

Query: 424 KTEANENSAISEFQSEVVQLGAVLNGDHFLSSFPDEISKKMSVKEAHEYTKGAVSRFIRA 462
           KTEANENS +SEFQSEVVQL AVLNGDHFLSSFP+EIS+KM+VKEAH+YT+GAVSRFIRA
Sbjct: 434 KTEANENSRLSEFQSEVVQLAAVLNGDHFLSSFPNEISEKMTVKEAHDYTRGAVSRFIRA 493

BLAST of Sed0002234 vs. NCBI nr
Match: XP_022987132.1 (non-specific phospholipase C6-like [Cucurbita maxima])

HSP 1 Score: 809.7 bits (2090), Expect = 1.3e-230
Identity = 411/508 (80.91%), Postives = 436/508 (85.83%), Query Frame = 0

Query: 4   FGFFLLIFLSLSWLPQPSFQQQPIQTVVVLVLENRSFDHMLGWMKKYMDDSRINGVTGHE 63
           F FFLL  L+LSW+PQ +FQQQPI+TVVVLV+ENRSFDHM+GWMKKY+ D RINGVTG E
Sbjct: 14  FIFFLL--LTLSWIPQRTFQQQPIKTVVVLVMENRSFDHMIGWMKKYI-DPRINGVTGDE 73

Query: 64  CNPVSTNNPNPQTVCFSDDAEFVDPDPGHSFEAVLHQVFGSGSIPSMSGFVEQALSISPN 123
           CNPVST NPNP+T+CFSDDAEFVDPDPGHSFE VL QVFGS SIPSMSGFVEQALS+S N
Sbjct: 74  CNPVSTKNPNPETICFSDDAEFVDPDPGHSFEDVLQQVFGSNSIPSMSGFVEQALSMSSN 133

Query: 124 LSQAVMKGFKPQSLPVYAALVPEFAVFDRWFSSIPGPTQPNRLFVYSATSHGSTSHLKKQ 183
           LS+ VMKGFKP+++P+Y ALV EFAVFDRWFSSIPGPTQPNRLFVYSATSHGSTSH+KKQ
Sbjct: 134 LSETVMKGFKPEAVPIYGALVREFAVFDRWFSSIPGPTQPNRLFVYSATSHGSTSHVKKQ 193

Query: 184 LAVGYPQKTIFDSLHENGLDFGIYFQNIPTTLFYRNLRKLKYIFKFHDYDLKFKKDATNG 243
           LA+GYPQKTIFDSLHENGLDFG+YFQNIPTTLFYRNLRKLKYIFKFH YDL+FKKDA NG
Sbjct: 194 LALGYPQKTIFDSLHENGLDFGVYFQNIPTTLFYRNLRKLKYIFKFHPYDLQFKKDARNG 253

Query: 244 KLPSLTVIEPRYFDLVGMPANDDHPSHDVANGQKLVKEVYETLRASPQWNQTLLIITYDE 303
           KLPSLTVIEPRYFDLVGMPANDDHPSHDVANGQKLVKEVYETLRASPQWNQTLLIITYDE
Sbjct: 254 KLPSLTVIEPRYFDLVGMPANDDHPSHDVANGQKLVKEVYETLRASPQWNQTLLIITYDE 313

Query: 304 H--------------------------------------------------VISSPKGPT 363
           H                                                  VISSPKGPT
Sbjct: 314 HGGFFDHVKTPFVNVPNPDGNTGPAPYFFKFDRLGVRVPTIMVSPWIKKGTVISSPKGPT 373

Query: 364 PNSEFELSSIPATIKKIFNLSSNFLTHRDAWAGTFEDIVGQLTSPRTDCPVTLPEVTALR 423
           PNSEFE SSIPATIKKIFNLSSNFLTHRDAWAGTFEDIVGQLTSPRTDCPVTLPEVT LR
Sbjct: 374 PNSEFEHSSIPATIKKIFNLSSNFLTHRDAWAGTFEDIVGQLTSPRTDCPVTLPEVTPLR 433

Query: 424 KTEANENSAISEFQSEVVQLGAVLNGDHFLSSFPDEISKKMSVKEAHEYTKGAVSRFIRA 462
           KTEA ENS +SEFQSEVVQL AVLNGDHFLSSFP+EIS+KM+VKEAH+YT+GAVSRFIRA
Sbjct: 434 KTEAKENSGLSEFQSEVVQLAAVLNGDHFLSSFPNEISEKMTVKEAHDYTRGAVSRFIRA 493

BLAST of Sed0002234 vs. NCBI nr
Match: KAG6589378.1 (Non-specific phospholipase C6, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 807.4 bits (2084), Expect = 6.5e-230
Identity = 411/508 (80.91%), Postives = 436/508 (85.83%), Query Frame = 0

Query: 4   FGFFLLIFLSLSWLPQPSFQQQPIQTVVVLVLENRSFDHMLGWMKKYMDDSRINGVTGHE 63
           F FFLL  L+LS +PQ +FQQQPI+TVVVLV+ENRSFDHM+GWMKKY+ D RINGVTG E
Sbjct: 14  FIFFLL--LTLSCIPQRTFQQQPIKTVVVLVMENRSFDHMIGWMKKYI-DPRINGVTGDE 73

Query: 64  CNPVSTNNPNPQTVCFSDDAEFVDPDPGHSFEAVLHQVFGSGSIPSMSGFVEQALSISPN 123
           CNPVST NPNP+T+CFSDDAEFVDPDPGHSFE VL QVFGS SIPSMSGFVEQALS+S N
Sbjct: 74  CNPVSTKNPNPETICFSDDAEFVDPDPGHSFEDVLQQVFGSNSIPSMSGFVEQALSMSSN 133

Query: 124 LSQAVMKGFKPQSLPVYAALVPEFAVFDRWFSSIPGPTQPNRLFVYSATSHGSTSHLKKQ 183
           LS+ VMKGFKP+++P+Y ALV EFAVFDRWFSSIPGPTQPNRLFVYSATSHGSTSH+KKQ
Sbjct: 134 LSETVMKGFKPEAVPIYGALVREFAVFDRWFSSIPGPTQPNRLFVYSATSHGSTSHVKKQ 193

Query: 184 LAVGYPQKTIFDSLHENGLDFGIYFQNIPTTLFYRNLRKLKYIFKFHDYDLKFKKDATNG 243
           LA+GYPQKTIFDSLHENGLDFG+YFQNIPTTLFYRNLRKLKYIFKFH YDL+FKKDA NG
Sbjct: 194 LALGYPQKTIFDSLHENGLDFGVYFQNIPTTLFYRNLRKLKYIFKFHPYDLQFKKDARNG 253

Query: 244 KLPSLTVIEPRYFDLVGMPANDDHPSHDVANGQKLVKEVYETLRASPQWNQTLLIITYDE 303
           KLPSLTVIEPRYFDLVGMPANDDHPSHDVANGQKLVKEVYETLRASPQWNQTLLIITYDE
Sbjct: 254 KLPSLTVIEPRYFDLVGMPANDDHPSHDVANGQKLVKEVYETLRASPQWNQTLLIITYDE 313

Query: 304 H--------------------------------------------------VISSPKGPT 363
           H                                                  VISSPKGPT
Sbjct: 314 HGGFFDHVRTPFVNVPNPDGNTGPAPYFFKFDRLGVRVPTIMVSPWIKKGTVISSPKGPT 373

Query: 364 PNSEFELSSIPATIKKIFNLSSNFLTHRDAWAGTFEDIVGQLTSPRTDCPVTLPEVTALR 423
           PNSEFE SSIPATIKKIFNLSSNFLTHRDAWAGTFEDIVGQLTSPRTDCPVTLPEVT LR
Sbjct: 374 PNSEFEHSSIPATIKKIFNLSSNFLTHRDAWAGTFEDIVGQLTSPRTDCPVTLPEVTPLR 433

Query: 424 KTEANENSAISEFQSEVVQLGAVLNGDHFLSSFPDEISKKMSVKEAHEYTKGAVSRFIRA 462
           KTEANENS +SEFQSEVVQL AVLNGDHFLSSFP+EIS+KM+VKEAH+YT+GAVSRFIRA
Sbjct: 434 KTEANENSGVSEFQSEVVQLAAVLNGDHFLSSFPNEISEKMTVKEAHDYTRGAVSRFIRA 493

BLAST of Sed0002234 vs. NCBI nr
Match: XP_023531289.1 (non-specific phospholipase C6-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 806.6 bits (2082), Expect = 1.1e-229
Identity = 407/503 (80.91%), Postives = 430/503 (85.49%), Query Frame = 0

Query: 9   LIFLSLSWLPQPSFQQQPIQTVVVLVLENRSFDHMLGWMKKYMDDSRINGVTGHECNPVS 68
           L+FL+LSWLPQPSFQQQPI+TVVVLV+ENRSFDHM+GWMKK++ + +INGVTG ECNP+S
Sbjct: 17  LLFLTLSWLPQPSFQQQPIKTVVVLVMENRSFDHMIGWMKKHI-NPQINGVTGDECNPLS 76

Query: 69  TNNPNPQTVCFSDDAEFVDPDPGHSFEAVLHQVFGSGSIPSMSGFVEQALSISPNLSQAV 128
           T NPNPQT+CFSDDA+FVDPDPGHSFEAVLHQVFGS SIPSMSGFVEQALS+SPNLSQ V
Sbjct: 77  TKNPNPQTICFSDDAQFVDPDPGHSFEAVLHQVFGSNSIPSMSGFVEQALSMSPNLSQTV 136

Query: 129 MKGFKPQSLPVYAALVPEFAVFDRWFSSIPGPTQPNRLFVYSATSHGSTSHLKKQLAVGY 188
           MKGF P+++PVY ALV EFAVFDRWFSSIPGPTQPNRLFVYSATSHGSTSH+KKQLA GY
Sbjct: 137 MKGFNPEAVPVYGALVREFAVFDRWFSSIPGPTQPNRLFVYSATSHGSTSHVKKQLAQGY 196

Query: 189 PQKTIFDSLHENGLDFGIYFQNIPTTLFYRNLRKLKYIFKFHDYDLKFKKDATNGKLPSL 248
           PQKTIFDSLHENGL+FGIYFQNIPTTLFYRNLRKLKYIFKFH YDLKFKKDA NGKLPSL
Sbjct: 197 PQKTIFDSLHENGLNFGIYFQNIPTTLFYRNLRKLKYIFKFHQYDLKFKKDARNGKLPSL 256

Query: 249 TVIEPRYFDLVGMPANDDHPSHDVANGQKLVKEVYETLRASPQWNQTLLIITYDEH---- 308
           TVIEPRYFDLVGMPANDDHPSHDVANGQKLVKEVYETLRASPQWNQTL IITYDEH    
Sbjct: 257 TVIEPRYFDLVGMPANDDHPSHDVANGQKLVKEVYETLRASPQWNQTLFIITYDEHGGFY 316

Query: 309 ----------------------------------------------VISSPKGPTPNSEF 368
                                                         VISSPKGPTPNSEF
Sbjct: 317 DHVNTPFVNVPNPDGNTGPPPYFFKFDRLGVRVPTIMVSPWIKKGTVISSPKGPTPNSEF 376

Query: 369 ELSSIPATIKKIFNLSSNFLTHRDAWAGTFEDIVGQLTSPRTDCPVTLPEVTALRKTEAN 428
           E SSIPATIKKIFNLSSNFLTHRDAWA TFED+VGQL SPRTDCPVTLPEVT LRKTEAN
Sbjct: 377 EHSSIPATIKKIFNLSSNFLTHRDAWAATFEDVVGQLASPRTDCPVTLPEVTPLRKTEAN 436

Query: 429 ENSAISEFQSEVVQLGAVLNGDHFLSSFPDEISKKMSVKEAHEYTKGAVSRFIRASKEAI 462
           E S +SEFQSEVVQL AVL GDHFLSSFP+ IS+KMSVKEAHEY+KGAVSRFIRASKEAI
Sbjct: 437 EKSGLSEFQSEVVQLAAVLKGDHFLSSFPNGISEKMSVKEAHEYSKGAVSRFIRASKEAI 496

BLAST of Sed0002234 vs. ExPASy Swiss-Prot
Match: Q8H965 (Non-specific phospholipase C6 OS=Arabidopsis thaliana OX=3702 GN=NPC6 PE=2 SV=1)

HSP 1 Score: 686.4 bits (1770), Expect = 2.2e-196
Identity = 355/509 (69.74%), Postives = 397/509 (78.00%), Query Frame = 0

Query: 4   FGFFLLIF--LSLSWLPQPSFQ-QQPIQTVVVLVLENRSFDHMLGWMKKYMDDSRINGVT 63
           F  FL ++  L+ + + Q S Q Q PI+TVVVLVLENRSFDH+LGWMK  ++ + INGVT
Sbjct: 13  FSHFLTLYCLLTQTHVAQGSHQWQSPIKTVVVLVLENRSFDHLLGWMKNSVNPT-INGVT 72

Query: 64  GHECNPVSTNNPNPQTVCFSDDAEFVDPDPGHSFEAVLHQVFGS--GSIPSMSGFVEQAL 123
           G ECNPV  +    QT+CF+ DAEFVDPDPGHSFEAV  QVFGS  G IPSM GFVEQAL
Sbjct: 73  GQECNPVPNST---QTICFTSDAEFVDPDPGHSFEAVEQQVFGSGPGQIPSMMGFVEQAL 132

Query: 124 SISPNLSQAVMKGFKPQSLPVYAALVPEFAVFDRWFSSIPGPTQPNRLFVYSATSHGSTS 183
           S+  NLS+ VMKGF+P+++PVYA LV EFAVFDRWFSSIPGPTQPNRLFVYSATSHGSTS
Sbjct: 133 SMPGNLSETVMKGFRPEAVPVYAELVKEFAVFDRWFSSIPGPTQPNRLFVYSATSHGSTS 192

Query: 184 HLKKQLAVGYPQKTIFDSLHENGLDFGIYFQNIPTTLFYRNLRKLKYIFKFHDYDLKFKK 243
           H+KKQLA GYPQKTIFDSLH N +DFGIYFQNIPTTLFYRNLR+LKYIF  H YDLKFKK
Sbjct: 193 HVKKQLAQGYPQKTIFDSLHSNDIDFGIYFQNIPTTLFYRNLRQLKYIFNLHQYDLKFKK 252

Query: 244 DATNGKLPSLTVIEPRYFDLVGMPANDDHPSHDVANGQKLVKEVYETLRASPQWNQTLLI 303
           DA  GKLPSLTVIEPRYFDL G+PANDDHPSHDVANGQKLVKEVYE LR+SPQWN+TLL+
Sbjct: 253 DAAKGKLPSLTVIEPRYFDLKGLPANDDHPSHDVANGQKLVKEVYEALRSSPQWNETLLV 312

Query: 304 ITYDEH--------------------------------------------------VISS 363
           ITYDEH                                                  V+S 
Sbjct: 313 ITYDEHGGFYDHVKTPYVGIPNPDGNTGPAPGFFKFDRLGVRVPTIMVSPWIQKGTVVSE 372

Query: 364 PKGPTPNSEFELSSIPATIKKIFNLSSNFLTHRDAWAGTFEDIVGQLTSPRTDCPVTLPE 423
            KGPT +SE+E SSIPATIKK+FNLSSNFLTHRDAWA TFED+V  LT+PRTDCP+TLPE
Sbjct: 373 AKGPTESSEYEHSSIPATIKKLFNLSSNFLTHRDAWAATFEDVVSHLTTPRTDCPMTLPE 432

Query: 424 VTALRKTEANENSAISEFQSEVVQLGAVLNGDHFLSSFPDEISKKMSVKEAHEYTKGAVS 458
           V  +R TE  E++A+SEFQ EVVQL AVLNGDHFLSSFP+EI KKM+VK+AHEY KGA S
Sbjct: 433 VAPMRATEPKEDAALSEFQGEVVQLAAVLNGDHFLSSFPEEIGKKMTVKQAHEYVKGATS 492

BLAST of Sed0002234 vs. ExPASy Swiss-Prot
Match: Q8L7Y9 (Non-specific phospholipase C1 OS=Arabidopsis thaliana OX=3702 GN=NPC1 PE=2 SV=1)

HSP 1 Score: 506.5 bits (1303), Expect = 3.1e-142
Identity = 261/495 (52.73%), Postives = 335/495 (67.68%), Query Frame = 0

Query: 19  QPSFQQQPIQTVVVLVLENRSFDHMLGWMKKYMDDSRINGVTGHECNPVSTNNPNPQTVC 78
           +P   Q PI+T+VV+V+ENRSFDH+LGW+K    +  I+G+TG E NP++ ++PN + + 
Sbjct: 30  KPHKIQGPIKTIVVVVMENRSFDHILGWLKSTRPE--IDGLTGKESNPLNVSDPNSKKIF 89

Query: 79  FSDDAEFVDPDPGHSFEAVLHQVFGSGSI---PSMSGFVEQALSISPNLSQAVMKGFKPQ 138
            SDDA FVD DPGHSF+A+  Q+FGS      P M+GF +Q+ S+ P +++ VM GFKP+
Sbjct: 90  VSDDAVFVDMDPGHSFQAIREQIFGSNDTSGDPKMNGFAQQSESMEPGMAKNVMSGFKPE 149

Query: 139 SLPVYAALVPEFAVFDRWFSSIPGPTQPNRLFVYSATSHGSTSHLKKQLAVGYPQKTIFD 198
            LPVY  L  EF VFDRWF+S+P  TQPNR +V+SATSHG +S++KK L  G+PQKTIFD
Sbjct: 150 VLPVYTELANEFGVFDRWFASVPTSTQPNRFYVHSATSHGCSSNVKKDLVKGFPQKTIFD 209

Query: 199 SLHENGLDFGIYFQNIPTTLFYRNLRKLKYIFKFHDYDLKFKKDATNGKLPSLTVIEPRY 258
           SL ENGL FGIY+QNIP T F+++LR+LK++ KFH Y LKFK DA  GKLP+ +V+E RY
Sbjct: 210 SLDENGLSFGIYYQNIPATFFFKSLRRLKHLVKFHSYALKFKLDAKLGKLPNYSVVEQRY 269

Query: 259 FDLVGMPANDDHPSHDVANGQKLVKEVYETLRASPQWNQTLLIITYDEH----------- 318
           FD+   PANDDHPSHDVA GQ+ VKEVYETLR+SPQW +  L+ITYDEH           
Sbjct: 270 FDIDLFPANDDHPSHDVAAGQRFVKEVYETLRSSPQWKEMALLITYDEHGGFYDHVPTPV 329

Query: 319 ---------------------------------------VISSPKGPTPNSEFELSSIPA 378
                                                  VI  P+GPTP+S+FE SSIPA
Sbjct: 330 KGVPNPDGIIGPDPFYFGFDRLGVRVPTFLISPWIEKGTVIHEPEGPTPHSQFEHSSIPA 389

Query: 379 TIKKIFNLSSNFLTHRDAWAGTFEDIVGQLTSPRTDCPVTLPEV-TALRKTEANENSAIS 438
           T+KK+FNL S+FLT RDAWAGTFE       SPR DCP  LPEV  +LR   A E+S +S
Sbjct: 390 TVKKLFNLKSHFLTKRDAWAGTFEKYFRIRDSPRQDCPEKLPEVKLSLRPWGAKEDSKLS 449

Query: 439 EFQSEVVQLGAVLNGDHFLSSFPDEISKKMSVKEAHEYTKGAVSRFIRASKEAIKLGADE 460
           EFQ E++QL + L GDH L+S+PD I K M+V E ++Y + AV +F+ A   A++ GADE
Sbjct: 450 EFQVELIQLASQLVGDHLLNSYPD-IGKNMTVSEGNKYAEDAVQKFLEAGMAALEAGADE 509

BLAST of Sed0002234 vs. ExPASy Swiss-Prot
Match: O81020 (Non-specific phospholipase C2 OS=Arabidopsis thaliana OX=3702 GN=NPC2 PE=2 SV=1)

HSP 1 Score: 500.7 bits (1288), Expect = 1.7e-140
Identity = 265/509 (52.06%), Postives = 334/509 (65.62%), Query Frame = 0

Query: 6   FFLLIFLSLSWLPQPSFQQQPIQTVVVLVLENRSFDHMLGWMKKYMDDSRINGVTGHECN 65
           F L+  LS++ L        PI+T+VV+V+ENRSFDHMLGWMKK   +  INGV G E N
Sbjct: 6   FALIQLLSVTILYNHVHATSPIKTIVVVVMENRSFDHMLGWMKKL--NPEINGVDGSESN 65

Query: 66  PVSTNNPNPQTVCFSDDAEFVDPDPGHSFEAVLHQVFGSGSI----PSMSGFVEQALSIS 125
           PVS ++P+ + + F   + +VDPDPGHSF+A+  QVFGS       P M+GFV+QA S  
Sbjct: 66  PVSVSDPSSRKIKFGSGSHYVDPDPGHSFQAIREQVFGSNDTSMDPPPMNGFVQQAYSED 125

Query: 126 P--NLSQAVMKGFKPQSLPVYAALVPEFAVFDRWFSSIPGPTQPNRLFVYSATSHGSTSH 185
           P  N+S +VM GF+P  +PVY +LV EFAVFDRWF+S+P  TQPNR+FV+S TS G+TS+
Sbjct: 126 PSGNMSASVMNGFEPDKVPVYKSLVSEFAVFDRWFASVPSSTQPNRMFVHSGTSAGATSN 185

Query: 186 LKKQLAVGYPQKTIFDSLHENGLDFGIYFQNIPTTLFYRNLRKLKYIFKFHDYDLKFKKD 245
               LA GYPQ+TIFD+L +    FGIY+QNIP  LFY++LRKLKY+FKFH Y   FK  
Sbjct: 186 NPISLAKGYPQRTIFDNLDDEEFSFGIYYQNIPAVLFYQSLRKLKYVFKFHSYGNSFKDH 245

Query: 246 ATNGKLPSLTVIEPRYFDLVGMPANDDHPSHDVANGQKLVKEVYETLRASPQWNQTLLII 305
           A  GKLP+ TVIE RY D +  PA+DDHPSHDV  GQK +KEVYETLRASPQWN+TLLII
Sbjct: 246 AKQGKLPAYTVIEQRYMDTLLEPASDDHPSHDVYQGQKFIKEVYETLRASPQWNETLLII 305

Query: 306 TYDEH--------------------------------------------------VISSP 365
           TYDEH                                                  V+  P
Sbjct: 306 TYDEHGGYFDHVPTPVRNVPSPDGIVGPDPFLFQFNRLGIRVPTIAVSPWIEKGTVVHGP 365

Query: 366 KG-PTPNSEFELSSIPATIKKIFNLSSNFLTHRDAWAGTFEDIVGQLTSPRTDCPVTLPE 425
            G P P+SE+E SSIPAT+KK+FNLSS FLT RD WAGTFE+I+     PRTDCP TLPE
Sbjct: 366 NGSPFPSSEYEHSSIPATVKKLFNLSSPFLTKRDEWAGTFENILQIRKEPRTDCPETLPE 425

Query: 426 VTALRKTEANENSAISEFQSEVVQLGAVLNGDHFLSSFPDEISKKMSVKEAHEYTKGAVS 458
              +R  EANE + ++EFQ E+VQL AVL GD+ L++FP EISK M+V E   Y + A+ 
Sbjct: 426 PVKIRMGEANEKALLTEFQQELVQLAAVLKGDNMLTTFPKEISKGMTVIEGKRYMEDAMK 485

BLAST of Sed0002234 vs. ExPASy Swiss-Prot
Match: Q9SRQ6 (Non-specific phospholipase C3 OS=Arabidopsis thaliana OX=3702 GN=NPC3 PE=1 SV=1)

HSP 1 Score: 435.3 bits (1118), Expect = 8.9e-121
Identity = 232/497 (46.68%), Postives = 308/497 (61.97%), Query Frame = 0

Query: 26  PIQTVVVLVLENRSFDHMLGWMKKYMDDSRINGVTGHE--CNPVSTNNPNPQTVCFSDDA 85
           PI+T+VVLV ENRSFDHMLGW K+   +  I+GV+  E   NP+ST++PN   + F  ++
Sbjct: 15  PIKTIVVLVQENRSFDHMLGWFKEL--NPEIDGVSESEPRSNPLSTSDPNSAQIFFGKES 74

Query: 86  EFVDPDPGHSFEAVLHQVFGSG-------SIPSMSGFVEQALSISPNLSQ-AVMKGFKPQ 145
           + +DPDPGHSF+A+  QVFG           P M+GFV+ A +I+  +S+  VM+GF P+
Sbjct: 75  QNIDPDPGHSFQAIYEQVFGKPFSDESPYPDPKMNGFVQNAEAITKGMSEKVVMQGFPPE 134

Query: 146 SLPVYAALVPEFAVFDRWFSSIPGPTQPNRLFVYSATSHGSTSHLKKQLAVGYPQKTIFD 205
            LPV+  LV EFAV DRWFSS+P  TQPNRL+V++ATS+G+ S+    L  G+PQ+T+F+
Sbjct: 135 KLPVFKELVQEFAVCDRWFSSLPSSTQPNRLYVHAATSNGAFSNDTNTLVRGFPQRTVFE 194

Query: 206 SLHENGLDFGIYFQNIPTTLFYRNLRKLKYIFKFHDYDLKFKKDATNGKLPSLTVIEPRY 265
           SL E+G  FGIY+Q+ P  LFYRN+RKLKY+  FH Y L FK+    GKLP+  VIEPRY
Sbjct: 195 SLEESGFTFGIYYQSFPNCLFYRNMRKLKYVDNFHQYHLSFKRHCKEGKLPNYVVIEPRY 254

Query: 266 FDLVGMPANDDHPSHDVANGQKLVKEVYETLRASPQWNQTLLIITYDEH----------- 325
           F ++  PANDDHP +DV  GQ LVKE+YE LRASPQWN+ L ++ YDEH           
Sbjct: 255 FKILSAPANDDHPKNDVVEGQNLVKEIYEALRASPQWNEILFVVVYDEHGGYYDHVPTPV 314

Query: 326 ---------------------------------------VISSPKGPTPNSEFELSSIPA 385
                                                  V+  P GP P S+FE SSIPA
Sbjct: 315 IGVPNPDGLVGPEPYNFKFDRLGVRVPALLISPWIEPGTVLHEPNGPEPTSQFEHSSIPA 374

Query: 386 TIKKIFNLSSNFLTHRDAWAGTFEDIVGQLTSPRTDCPVTLPEVTALRKTE---ANENSA 445
           T+KKIFNL S FLT RD WAGT + ++ + TSPRTDCPVTLPE+   R  +     E+  
Sbjct: 375 TLKKIFNLKS-FLTKRDEWAGTLDAVINR-TSPRTDCPVTLPELPRARDIDIGTQEEDED 434

Query: 446 ISEFQSEVVQLGAVLNGDHFLSSFPDEISKKMSVKEAHEYTKGAVSRFIRASKEAIKLGA 460
           +++FQ E++Q  AVL GDH    +P +++ KM V +A  Y + A +RF   SK+A + G 
Sbjct: 435 LTDFQIELIQAAAVLKGDHIKDIYPFKLADKMKVLDAARYVEEAFTRFHGESKKAKEEGR 494

BLAST of Sed0002234 vs. ExPASy Swiss-Prot
Match: Q9SRQ7 (Non-specific phospholipase C4 OS=Arabidopsis thaliana OX=3702 GN=NPC4 PE=1 SV=1)

HSP 1 Score: 427.9 bits (1099), Expect = 1.4e-118
Identity = 232/485 (47.84%), Postives = 298/485 (61.44%), Query Frame = 0

Query: 26  PIQTVVVLVLENRSFDHMLGWMKKYMDDSRINGVTGHE--CNPVSTNNPNPQTVCFSDDA 85
           PI+T+VVLV ENRSFDH LGW K+   +  I+GVT  +   N VS+++ N   V F D +
Sbjct: 13  PIKTIVVLVQENRSFDHTLGWFKEL--NREIDGVTKSDPKSNTVSSSDTNSLRVVFGDQS 72

Query: 86  EFVDPDPGHSFEAVLHQVFG----------SGSIPSMSGFVEQALSISPNLSQAVMKGFK 145
           ++V+PDPGHS + +  QVFG          +   P+MSGF + A      +S AVM GFK
Sbjct: 73  QYVNPDPGHSIQDIYEQVFGKPWDSGKPDPNPGHPNMSGFAQNAERNKKGMSSAVMNGFK 132

Query: 146 PQSLPVYAALVPEFAVFDRWFSSIPGPTQPNRLFVYSATSHGSTSHLKKQLAVGYPQKTI 205
           P +LPVY  LV  FA+ DRWF+S+P  TQPNRL+V+SATSHG+TS+ KK L  G+PQKTI
Sbjct: 133 PNALPVYKELVQNFAICDRWFASVPASTQPNRLYVHSATSHGATSNDKKLLLEGFPQKTI 192

Query: 206 FDSLHENGLDFGIYFQNIPTTLFYRNLRKLKYIFKFHDYDLKFKKDATNGKLPSLTVIEP 265
           F+SL E G  FGIY+Q  P+TLFYRNLRKLKY+  FH Y ++FKKD   GKLP+  V+E 
Sbjct: 193 FESLDEAGFSFGIYYQFPPSTLFYRNLRKLKYLTHFHQYGIQFKKDCKEGKLPNYVVVEQ 252

Query: 266 RYFDLVGMPANDDHPSHDVANGQKLVKEVYETLRASPQWNQTLLIITYDEH--------- 325
           R+FDL+  PANDDHPSHDV+ GQKLVKEVYE LR+SPQWN+ L IITYDEH         
Sbjct: 253 RWFDLLSTPANDDHPSHDVSEGQKLVKEVYEALRSSPQWNEILFIITYDEHGGFYDHVPT 312

Query: 326 -----------------------------------------VISSPKGPTPNSEFELSSI 385
                                                    VI  P GP P S++E SSI
Sbjct: 313 PVDGVPNPDGILGPPPYNFEFNRLGVRVPTFFISPWIEPGTVIHGPNGPYPRSQYEHSSI 372

Query: 386 PATIKKIFNLSSNFLTHRDAWAGTFEDIVGQLTSPRTDCPVTLPEVTALRKTEANENSAI 445
           PAT+K IF L  +FL+ RD+WAGTFE ++ +  SPR DCP TL     LR T A EN+ +
Sbjct: 373 PATVKTIFKL-KDFLSKRDSWAGTFESVITR-DSPRQDCPETLSTPIKLRGTMAKENAQL 432

Query: 446 SEFQSEVVQLGAVLNGDHFLSSFPDEISKKMSVKEAHEYTKGAVSRFIRASKEAIKLGAD 449
           SEFQ ++V + A L GD+       ++ K+  V +A +Y   A  +F+  S++A   G D
Sbjct: 433 SEFQEDLVIMAAGLKGDYKNEELIHKLCKETCVADASKYVTNAFEKFLEESRKARDRGCD 492

BLAST of Sed0002234 vs. ExPASy TrEMBL
Match: A0A6J1E0F1 (non-specific phospholipase C6-like OS=Cucurbita moschata OX=3662 GN=LOC111429704 PE=4 SV=1)

HSP 1 Score: 812.4 bits (2097), Expect = 9.8e-232
Identity = 412/508 (81.10%), Postives = 437/508 (86.02%), Query Frame = 0

Query: 4   FGFFLLIFLSLSWLPQPSFQQQPIQTVVVLVLENRSFDHMLGWMKKYMDDSRINGVTGHE 63
           F FFLL  L+LSW+PQ +FQQQPI+TVVVLV+ENRSFDHM+GWMKKY+ D RINGVTG E
Sbjct: 14  FIFFLL--LTLSWIPQRTFQQQPIKTVVVLVMENRSFDHMIGWMKKYI-DPRINGVTGDE 73

Query: 64  CNPVSTNNPNPQTVCFSDDAEFVDPDPGHSFEAVLHQVFGSGSIPSMSGFVEQALSISPN 123
           CNPVST NPNP+T+CFSDDAEFVDPDPGHSFE VL QVFGS SIPSMSGFVEQALS+S N
Sbjct: 74  CNPVSTKNPNPETICFSDDAEFVDPDPGHSFEDVLQQVFGSNSIPSMSGFVEQALSMSSN 133

Query: 124 LSQAVMKGFKPQSLPVYAALVPEFAVFDRWFSSIPGPTQPNRLFVYSATSHGSTSHLKKQ 183
           LS+ VMKGFKP+++P+Y ALV EFAVFDRWFSSIPGPTQPNRLFVYSATSHGSTSH+KKQ
Sbjct: 134 LSETVMKGFKPEAVPIYGALVREFAVFDRWFSSIPGPTQPNRLFVYSATSHGSTSHVKKQ 193

Query: 184 LAVGYPQKTIFDSLHENGLDFGIYFQNIPTTLFYRNLRKLKYIFKFHDYDLKFKKDATNG 243
           LA+GYPQKTIFDSLHENGLDFG+YFQNIPTTLFYRNLRKLKYIFKFH YDL+FKKDA NG
Sbjct: 194 LALGYPQKTIFDSLHENGLDFGVYFQNIPTTLFYRNLRKLKYIFKFHPYDLQFKKDARNG 253

Query: 244 KLPSLTVIEPRYFDLVGMPANDDHPSHDVANGQKLVKEVYETLRASPQWNQTLLIITYDE 303
           KLPSLTVIEPRYFDLVGMPANDDHPSHDVANGQKLVKEVYETLRASPQWNQTLLIITYDE
Sbjct: 254 KLPSLTVIEPRYFDLVGMPANDDHPSHDVANGQKLVKEVYETLRASPQWNQTLLIITYDE 313

Query: 304 H--------------------------------------------------VISSPKGPT 363
           H                                                  VISSPKGPT
Sbjct: 314 HGGFFDHVRTPFVNVPNPDGNTGPAPYFFKFDRLGVRVPTIMVSPWIKKGTVISSPKGPT 373

Query: 364 PNSEFELSSIPATIKKIFNLSSNFLTHRDAWAGTFEDIVGQLTSPRTDCPVTLPEVTALR 423
           PNSEFE SSIPATIKKIFNLSSNFLTHRDAWAGTFEDIVGQLTSPRTDCPVTLPEVT LR
Sbjct: 374 PNSEFEHSSIPATIKKIFNLSSNFLTHRDAWAGTFEDIVGQLTSPRTDCPVTLPEVTPLR 433

Query: 424 KTEANENSAISEFQSEVVQLGAVLNGDHFLSSFPDEISKKMSVKEAHEYTKGAVSRFIRA 462
           KTEANENS +SEFQSEVVQL AVLNGDHFLSSFP+EIS+KM+VKEAH+YT+GAVSRFIRA
Sbjct: 434 KTEANENSGVSEFQSEVVQLAAVLNGDHFLSSFPNEISEKMTVKEAHDYTRGAVSRFIRA 493

BLAST of Sed0002234 vs. ExPASy TrEMBL
Match: A0A6J1J9I6 (non-specific phospholipase C6-like OS=Cucurbita maxima OX=3661 GN=LOC111484775 PE=4 SV=1)

HSP 1 Score: 809.7 bits (2090), Expect = 6.4e-231
Identity = 411/508 (80.91%), Postives = 436/508 (85.83%), Query Frame = 0

Query: 4   FGFFLLIFLSLSWLPQPSFQQQPIQTVVVLVLENRSFDHMLGWMKKYMDDSRINGVTGHE 63
           F FFLL  L+LSW+PQ +FQQQPI+TVVVLV+ENRSFDHM+GWMKKY+ D RINGVTG E
Sbjct: 14  FIFFLL--LTLSWIPQRTFQQQPIKTVVVLVMENRSFDHMIGWMKKYI-DPRINGVTGDE 73

Query: 64  CNPVSTNNPNPQTVCFSDDAEFVDPDPGHSFEAVLHQVFGSGSIPSMSGFVEQALSISPN 123
           CNPVST NPNP+T+CFSDDAEFVDPDPGHSFE VL QVFGS SIPSMSGFVEQALS+S N
Sbjct: 74  CNPVSTKNPNPETICFSDDAEFVDPDPGHSFEDVLQQVFGSNSIPSMSGFVEQALSMSSN 133

Query: 124 LSQAVMKGFKPQSLPVYAALVPEFAVFDRWFSSIPGPTQPNRLFVYSATSHGSTSHLKKQ 183
           LS+ VMKGFKP+++P+Y ALV EFAVFDRWFSSIPGPTQPNRLFVYSATSHGSTSH+KKQ
Sbjct: 134 LSETVMKGFKPEAVPIYGALVREFAVFDRWFSSIPGPTQPNRLFVYSATSHGSTSHVKKQ 193

Query: 184 LAVGYPQKTIFDSLHENGLDFGIYFQNIPTTLFYRNLRKLKYIFKFHDYDLKFKKDATNG 243
           LA+GYPQKTIFDSLHENGLDFG+YFQNIPTTLFYRNLRKLKYIFKFH YDL+FKKDA NG
Sbjct: 194 LALGYPQKTIFDSLHENGLDFGVYFQNIPTTLFYRNLRKLKYIFKFHPYDLQFKKDARNG 253

Query: 244 KLPSLTVIEPRYFDLVGMPANDDHPSHDVANGQKLVKEVYETLRASPQWNQTLLIITYDE 303
           KLPSLTVIEPRYFDLVGMPANDDHPSHDVANGQKLVKEVYETLRASPQWNQTLLIITYDE
Sbjct: 254 KLPSLTVIEPRYFDLVGMPANDDHPSHDVANGQKLVKEVYETLRASPQWNQTLLIITYDE 313

Query: 304 H--------------------------------------------------VISSPKGPT 363
           H                                                  VISSPKGPT
Sbjct: 314 HGGFFDHVKTPFVNVPNPDGNTGPAPYFFKFDRLGVRVPTIMVSPWIKKGTVISSPKGPT 373

Query: 364 PNSEFELSSIPATIKKIFNLSSNFLTHRDAWAGTFEDIVGQLTSPRTDCPVTLPEVTALR 423
           PNSEFE SSIPATIKKIFNLSSNFLTHRDAWAGTFEDIVGQLTSPRTDCPVTLPEVT LR
Sbjct: 374 PNSEFEHSSIPATIKKIFNLSSNFLTHRDAWAGTFEDIVGQLTSPRTDCPVTLPEVTPLR 433

Query: 424 KTEANENSAISEFQSEVVQLGAVLNGDHFLSSFPDEISKKMSVKEAHEYTKGAVSRFIRA 462
           KTEA ENS +SEFQSEVVQL AVLNGDHFLSSFP+EIS+KM+VKEAH+YT+GAVSRFIRA
Sbjct: 434 KTEAKENSGLSEFQSEVVQLAAVLNGDHFLSSFPNEISEKMTVKEAHDYTRGAVSRFIRA 493

BLAST of Sed0002234 vs. ExPASy TrEMBL
Match: A0A6J1L5I1 (non-specific phospholipase C6-like OS=Cucurbita maxima OX=3661 GN=LOC111500200 PE=4 SV=1)

HSP 1 Score: 805.4 bits (2079), Expect = 1.2e-229
Identity = 405/503 (80.52%), Postives = 431/503 (85.69%), Query Frame = 0

Query: 9   LIFLSLSWLPQPSFQQQPIQTVVVLVLENRSFDHMLGWMKKYMDDSRINGVTGHECNPVS 68
           L+FL+LSWLPQP+FQQQPI+TVVVLV+ENRSFDHM+GWMKK++ + +INGVTG ECNP+S
Sbjct: 17  LLFLTLSWLPQPTFQQQPIKTVVVLVMENRSFDHMIGWMKKHI-NPQINGVTGDECNPLS 76

Query: 69  TNNPNPQTVCFSDDAEFVDPDPGHSFEAVLHQVFGSGSIPSMSGFVEQALSISPNLSQAV 128
           T NPNPQT+CFSDDA+FVDPDPGHSFEAVLHQVFGS SIPSMSGFVEQALS+SPNLSQ V
Sbjct: 77  TKNPNPQTICFSDDAQFVDPDPGHSFEAVLHQVFGSNSIPSMSGFVEQALSMSPNLSQTV 136

Query: 129 MKGFKPQSLPVYAALVPEFAVFDRWFSSIPGPTQPNRLFVYSATSHGSTSHLKKQLAVGY 188
           MKGF P+++PVY ALV EFAVFDRWFSSIPGPTQPNRLFVYSATSHGSTSH+KKQLA GY
Sbjct: 137 MKGFNPEAVPVYGALVREFAVFDRWFSSIPGPTQPNRLFVYSATSHGSTSHVKKQLAQGY 196

Query: 189 PQKTIFDSLHENGLDFGIYFQNIPTTLFYRNLRKLKYIFKFHDYDLKFKKDATNGKLPSL 248
           PQKTIFDSLHENGL+FGIYFQNIPTTLFYRNLRKLKYIFKFH YDLKFKKDA NGKLPSL
Sbjct: 197 PQKTIFDSLHENGLNFGIYFQNIPTTLFYRNLRKLKYIFKFHQYDLKFKKDARNGKLPSL 256

Query: 249 TVIEPRYFDLVGMPANDDHPSHDVANGQKLVKEVYETLRASPQWNQTLLIITYDEH---- 308
           TVIEPRYFDLVGMPANDDHPSHDVANGQKLVKEVYETLR+SPQWNQTL IITYDEH    
Sbjct: 257 TVIEPRYFDLVGMPANDDHPSHDVANGQKLVKEVYETLRSSPQWNQTLFIITYDEHGGFF 316

Query: 309 ----------------------------------------------VISSPKGPTPNSEF 368
                                                         V+SSPKGPTPNSEF
Sbjct: 317 DHVNTPFVNVPNPDGNTGPPPYFFKFDRLGVRVPTIMVSPWIKKGTVLSSPKGPTPNSEF 376

Query: 369 ELSSIPATIKKIFNLSSNFLTHRDAWAGTFEDIVGQLTSPRTDCPVTLPEVTALRKTEAN 428
           E SSIPATIKKIFNLSSNFLTHRDAWA TFED+VGQL SPRTDCPVTLPEVT LRKTEAN
Sbjct: 377 EHSSIPATIKKIFNLSSNFLTHRDAWAATFEDVVGQLGSPRTDCPVTLPEVTPLRKTEAN 436

Query: 429 ENSAISEFQSEVVQLGAVLNGDHFLSSFPDEISKKMSVKEAHEYTKGAVSRFIRASKEAI 462
           E S +SEFQSEVVQL AVL GDHFLSSFP+EIS+KMSVKEAHEY+KGAVSRFIRASKEAI
Sbjct: 437 ERSGLSEFQSEVVQLAAVLKGDHFLSSFPNEISEKMSVKEAHEYSKGAVSRFIRASKEAI 496

BLAST of Sed0002234 vs. ExPASy TrEMBL
Match: A0A6J1EP58 (non-specific phospholipase C6-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111436442 PE=4 SV=1)

HSP 1 Score: 804.3 bits (2076), Expect = 2.7e-229
Identity = 405/503 (80.52%), Postives = 430/503 (85.49%), Query Frame = 0

Query: 9   LIFLSLSWLPQPSFQQQPIQTVVVLVLENRSFDHMLGWMKKYMDDSRINGVTGHECNPVS 68
           L+FL+LSWLPQPSFQQQPI+TVVVLV+ENRSFDHM+GWMKK++ + +INGVTG ECNP+S
Sbjct: 17  LLFLTLSWLPQPSFQQQPIKTVVVLVMENRSFDHMIGWMKKHI-NPQINGVTGDECNPLS 76

Query: 69  TNNPNPQTVCFSDDAEFVDPDPGHSFEAVLHQVFGSGSIPSMSGFVEQALSISPNLSQAV 128
           T NPNPQT+CFSDDA+FVDPDPGHSFEAVLHQVFGS SIPSMSGFVEQALS+SPNLSQ V
Sbjct: 77  TKNPNPQTICFSDDAQFVDPDPGHSFEAVLHQVFGSNSIPSMSGFVEQALSMSPNLSQTV 136

Query: 129 MKGFKPQSLPVYAALVPEFAVFDRWFSSIPGPTQPNRLFVYSATSHGSTSHLKKQLAVGY 188
           MKGF P+++PVY ALV EFAVFDRWFSSIPGPTQPNRLFVYSATSHGSTSH+KKQLA GY
Sbjct: 137 MKGFNPEAVPVYGALVREFAVFDRWFSSIPGPTQPNRLFVYSATSHGSTSHVKKQLAQGY 196

Query: 189 PQKTIFDSLHENGLDFGIYFQNIPTTLFYRNLRKLKYIFKFHDYDLKFKKDATNGKLPSL 248
           PQKTIFDSLHENGL+FGIYFQNIPTTLFYRNLRKLKYIFKFH YDLKFKKDA NGKLPSL
Sbjct: 197 PQKTIFDSLHENGLNFGIYFQNIPTTLFYRNLRKLKYIFKFHQYDLKFKKDARNGKLPSL 256

Query: 249 TVIEPRYFDLVGMPANDDHPSHDVANGQKLVKEVYETLRASPQWNQTLLIITYDEH---- 308
           TVIEPRYFDLVGMPANDDHPSHDVANGQKLVKEVYETLRASPQWNQTL IITYDEH    
Sbjct: 257 TVIEPRYFDLVGMPANDDHPSHDVANGQKLVKEVYETLRASPQWNQTLFIITYDEHGGFF 316

Query: 309 ----------------------------------------------VISSPKGPTPNSEF 368
                                                         VISSPKGPTPNSEF
Sbjct: 317 DHVNTPFVNVPNPDGNTGPPPYFFKFDRLGVRVPTIMVSPWIKKGTVISSPKGPTPNSEF 376

Query: 369 ELSSIPATIKKIFNLSSNFLTHRDAWAGTFEDIVGQLTSPRTDCPVTLPEVTALRKTEAN 428
           E SSIPATIKKIFNLSSNFLTHRDAWA TFED+VGQL SPRTDCPVTLPEVTALRKTEAN
Sbjct: 377 EHSSIPATIKKIFNLSSNFLTHRDAWAATFEDVVGQLASPRTDCPVTLPEVTALRKTEAN 436

Query: 429 ENSAISEFQSEVVQLGAVLNGDHFLSSFPDEISKKMSVKEAHEYTKGAVSRFIRASKEAI 462
           E S +SEFQSE+VQL AVL GDHFLSSFP+ IS+KMSV+ AHEY+KGAVSRFIRASKEAI
Sbjct: 437 EKSGLSEFQSEIVQLAAVLKGDHFLSSFPNGISEKMSVRGAHEYSKGAVSRFIRASKEAI 496

BLAST of Sed0002234 vs. ExPASy TrEMBL
Match: A0A5D3BH49 (Non-specific phospholipase C6 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold350G00480 PE=4 SV=1)

HSP 1 Score: 803.9 bits (2075), Expect = 3.5e-229
Identity = 406/506 (80.24%), Postives = 435/506 (85.97%), Query Frame = 0

Query: 6   FFLLIFLSLSWLPQPSFQQQPIQTVVVLVLENRSFDHMLGWMKKYMDDSRINGVTGHECN 65
           FFLL  L+LSWLPQ SFQQQPI+TVVVLV+ENRSFDHM+GWMKKY+ + +INGVTG ECN
Sbjct: 16  FFLL--LTLSWLPQRSFQQQPIKTVVVLVMENRSFDHMIGWMKKYV-NPKINGVTGDECN 75

Query: 66  PVSTNNPNPQTVCFSDDAEFVDPDPGHSFEAVLHQVFGSGSIPSMSGFVEQALSISPNLS 125
           PVST NPNP+T+CF+DDAEFVDPDPGHSFE VL QVFGS SIPSMSGFVEQALS+SPNLS
Sbjct: 76  PVSTKNPNPETICFTDDAEFVDPDPGHSFEDVLQQVFGSNSIPSMSGFVEQALSMSPNLS 135

Query: 126 QAVMKGFKPQSLPVYAALVPEFAVFDRWFSSIPGPTQPNRLFVYSATSHGSTSHLKKQLA 185
           + VMKGFKP+++P+Y ALV EFAVFDRWFSSIPGPTQPNRLFVYSATSHGSTSH+KKQLA
Sbjct: 136 ETVMKGFKPEAVPIYGALVREFAVFDRWFSSIPGPTQPNRLFVYSATSHGSTSHVKKQLA 195

Query: 186 VGYPQKTIFDSLHENGLDFGIYFQNIPTTLFYRNLRKLKYIFKFHDYDLKFKKDATNGKL 245
           +GYPQKTIFDSLHENG++FGIYFQNIPTTLFYRNLRKLKYIFKFH YDLKFKKDA NGKL
Sbjct: 196 MGYPQKTIFDSLHENGINFGIYFQNIPTTLFYRNLRKLKYIFKFHQYDLKFKKDARNGKL 255

Query: 246 PSLTVIEPRYFDLVGMPANDDHPSHDVANGQKLVKEVYETLRASPQWNQTLLIITYDEH- 305
           PSLTVIEPRYFDLVGMPANDDHPSHDVANGQKLVKEVYETLRASPQWN+TLLIITYDEH 
Sbjct: 256 PSLTVIEPRYFDLVGMPANDDHPSHDVANGQKLVKEVYETLRASPQWNETLLIITYDEHG 315

Query: 306 -------------------------------------------------VISSPKGPTPN 365
                                                            VISSPKGPTPN
Sbjct: 316 GFFDHVKTPFVNVPNPDGNTGPAPYFFKFDRLGVRVPTIMVSPWIKKGTVISSPKGPTPN 375

Query: 366 SEFELSSIPATIKKIFNLSSNFLTHRDAWAGTFEDIVGQLTSPRTDCPVTLPEVTALRKT 425
           SEFE SSIPATIKKIFN+ SNFLTHRDAWAGTFEDIV QLTSPRTDCPVTLPEVT LRKT
Sbjct: 376 SEFEHSSIPATIKKIFNIPSNFLTHRDAWAGTFEDIVDQLTSPRTDCPVTLPEVTPLRKT 435

Query: 426 EANENSAISEFQSEVVQLGAVLNGDHFLSSFPDEISKKMSVKEAHEYTKGAVSRFIRASK 462
           EANENS +SEFQSEVVQL AVLNGDHFLSSFP+EIS+KM+VKEAH+YT+GAVSRFIRASK
Sbjct: 436 EANENSGLSEFQSEVVQLAAVLNGDHFLSSFPNEISEKMTVKEAHDYTRGAVSRFIRASK 495

BLAST of Sed0002234 vs. TAIR 10
Match: AT3G48610.1 (non-specific phospholipase C6 )

HSP 1 Score: 686.4 bits (1770), Expect = 1.6e-197
Identity = 355/509 (69.74%), Postives = 397/509 (78.00%), Query Frame = 0

Query: 4   FGFFLLIF--LSLSWLPQPSFQ-QQPIQTVVVLVLENRSFDHMLGWMKKYMDDSRINGVT 63
           F  FL ++  L+ + + Q S Q Q PI+TVVVLVLENRSFDH+LGWMK  ++ + INGVT
Sbjct: 13  FSHFLTLYCLLTQTHVAQGSHQWQSPIKTVVVLVLENRSFDHLLGWMKNSVNPT-INGVT 72

Query: 64  GHECNPVSTNNPNPQTVCFSDDAEFVDPDPGHSFEAVLHQVFGS--GSIPSMSGFVEQAL 123
           G ECNPV  +    QT+CF+ DAEFVDPDPGHSFEAV  QVFGS  G IPSM GFVEQAL
Sbjct: 73  GQECNPVPNST---QTICFTSDAEFVDPDPGHSFEAVEQQVFGSGPGQIPSMMGFVEQAL 132

Query: 124 SISPNLSQAVMKGFKPQSLPVYAALVPEFAVFDRWFSSIPGPTQPNRLFVYSATSHGSTS 183
           S+  NLS+ VMKGF+P+++PVYA LV EFAVFDRWFSSIPGPTQPNRLFVYSATSHGSTS
Sbjct: 133 SMPGNLSETVMKGFRPEAVPVYAELVKEFAVFDRWFSSIPGPTQPNRLFVYSATSHGSTS 192

Query: 184 HLKKQLAVGYPQKTIFDSLHENGLDFGIYFQNIPTTLFYRNLRKLKYIFKFHDYDLKFKK 243
           H+KKQLA GYPQKTIFDSLH N +DFGIYFQNIPTTLFYRNLR+LKYIF  H YDLKFKK
Sbjct: 193 HVKKQLAQGYPQKTIFDSLHSNDIDFGIYFQNIPTTLFYRNLRQLKYIFNLHQYDLKFKK 252

Query: 244 DATNGKLPSLTVIEPRYFDLVGMPANDDHPSHDVANGQKLVKEVYETLRASPQWNQTLLI 303
           DA  GKLPSLTVIEPRYFDL G+PANDDHPSHDVANGQKLVKEVYE LR+SPQWN+TLL+
Sbjct: 253 DAAKGKLPSLTVIEPRYFDLKGLPANDDHPSHDVANGQKLVKEVYEALRSSPQWNETLLV 312

Query: 304 ITYDEH--------------------------------------------------VISS 363
           ITYDEH                                                  V+S 
Sbjct: 313 ITYDEHGGFYDHVKTPYVGIPNPDGNTGPAPGFFKFDRLGVRVPTIMVSPWIQKGTVVSE 372

Query: 364 PKGPTPNSEFELSSIPATIKKIFNLSSNFLTHRDAWAGTFEDIVGQLTSPRTDCPVTLPE 423
            KGPT +SE+E SSIPATIKK+FNLSSNFLTHRDAWA TFED+V  LT+PRTDCP+TLPE
Sbjct: 373 AKGPTESSEYEHSSIPATIKKLFNLSSNFLTHRDAWAATFEDVVSHLTTPRTDCPMTLPE 432

Query: 424 VTALRKTEANENSAISEFQSEVVQLGAVLNGDHFLSSFPDEISKKMSVKEAHEYTKGAVS 458
           V  +R TE  E++A+SEFQ EVVQL AVLNGDHFLSSFP+EI KKM+VK+AHEY KGA S
Sbjct: 433 VAPMRATEPKEDAALSEFQGEVVQLAAVLNGDHFLSSFPEEIGKKMTVKQAHEYVKGATS 492

BLAST of Sed0002234 vs. TAIR 10
Match: AT1G07230.1 (non-specific phospholipase C1 )

HSP 1 Score: 506.5 bits (1303), Expect = 2.2e-143
Identity = 261/495 (52.73%), Postives = 335/495 (67.68%), Query Frame = 0

Query: 19  QPSFQQQPIQTVVVLVLENRSFDHMLGWMKKYMDDSRINGVTGHECNPVSTNNPNPQTVC 78
           +P   Q PI+T+VV+V+ENRSFDH+LGW+K    +  I+G+TG E NP++ ++PN + + 
Sbjct: 30  KPHKIQGPIKTIVVVVMENRSFDHILGWLKSTRPE--IDGLTGKESNPLNVSDPNSKKIF 89

Query: 79  FSDDAEFVDPDPGHSFEAVLHQVFGSGSI---PSMSGFVEQALSISPNLSQAVMKGFKPQ 138
            SDDA FVD DPGHSF+A+  Q+FGS      P M+GF +Q+ S+ P +++ VM GFKP+
Sbjct: 90  VSDDAVFVDMDPGHSFQAIREQIFGSNDTSGDPKMNGFAQQSESMEPGMAKNVMSGFKPE 149

Query: 139 SLPVYAALVPEFAVFDRWFSSIPGPTQPNRLFVYSATSHGSTSHLKKQLAVGYPQKTIFD 198
            LPVY  L  EF VFDRWF+S+P  TQPNR +V+SATSHG +S++KK L  G+PQKTIFD
Sbjct: 150 VLPVYTELANEFGVFDRWFASVPTSTQPNRFYVHSATSHGCSSNVKKDLVKGFPQKTIFD 209

Query: 199 SLHENGLDFGIYFQNIPTTLFYRNLRKLKYIFKFHDYDLKFKKDATNGKLPSLTVIEPRY 258
           SL ENGL FGIY+QNIP T F+++LR+LK++ KFH Y LKFK DA  GKLP+ +V+E RY
Sbjct: 210 SLDENGLSFGIYYQNIPATFFFKSLRRLKHLVKFHSYALKFKLDAKLGKLPNYSVVEQRY 269

Query: 259 FDLVGMPANDDHPSHDVANGQKLVKEVYETLRASPQWNQTLLIITYDEH----------- 318
           FD+   PANDDHPSHDVA GQ+ VKEVYETLR+SPQW +  L+ITYDEH           
Sbjct: 270 FDIDLFPANDDHPSHDVAAGQRFVKEVYETLRSSPQWKEMALLITYDEHGGFYDHVPTPV 329

Query: 319 ---------------------------------------VISSPKGPTPNSEFELSSIPA 378
                                                  VI  P+GPTP+S+FE SSIPA
Sbjct: 330 KGVPNPDGIIGPDPFYFGFDRLGVRVPTFLISPWIEKGTVIHEPEGPTPHSQFEHSSIPA 389

Query: 379 TIKKIFNLSSNFLTHRDAWAGTFEDIVGQLTSPRTDCPVTLPEV-TALRKTEANENSAIS 438
           T+KK+FNL S+FLT RDAWAGTFE       SPR DCP  LPEV  +LR   A E+S +S
Sbjct: 390 TVKKLFNLKSHFLTKRDAWAGTFEKYFRIRDSPRQDCPEKLPEVKLSLRPWGAKEDSKLS 449

Query: 439 EFQSEVVQLGAVLNGDHFLSSFPDEISKKMSVKEAHEYTKGAVSRFIRASKEAIKLGADE 460
           EFQ E++QL + L GDH L+S+PD I K M+V E ++Y + AV +F+ A   A++ GADE
Sbjct: 450 EFQVELIQLASQLVGDHLLNSYPD-IGKNMTVSEGNKYAEDAVQKFLEAGMAALEAGADE 509

BLAST of Sed0002234 vs. TAIR 10
Match: AT2G26870.1 (non-specific phospholipase C2 )

HSP 1 Score: 500.7 bits (1288), Expect = 1.2e-141
Identity = 265/509 (52.06%), Postives = 334/509 (65.62%), Query Frame = 0

Query: 6   FFLLIFLSLSWLPQPSFQQQPIQTVVVLVLENRSFDHMLGWMKKYMDDSRINGVTGHECN 65
           F L+  LS++ L        PI+T+VV+V+ENRSFDHMLGWMKK   +  INGV G E N
Sbjct: 6   FALIQLLSVTILYNHVHATSPIKTIVVVVMENRSFDHMLGWMKKL--NPEINGVDGSESN 65

Query: 66  PVSTNNPNPQTVCFSDDAEFVDPDPGHSFEAVLHQVFGSGSI----PSMSGFVEQALSIS 125
           PVS ++P+ + + F   + +VDPDPGHSF+A+  QVFGS       P M+GFV+QA S  
Sbjct: 66  PVSVSDPSSRKIKFGSGSHYVDPDPGHSFQAIREQVFGSNDTSMDPPPMNGFVQQAYSED 125

Query: 126 P--NLSQAVMKGFKPQSLPVYAALVPEFAVFDRWFSSIPGPTQPNRLFVYSATSHGSTSH 185
           P  N+S +VM GF+P  +PVY +LV EFAVFDRWF+S+P  TQPNR+FV+S TS G+TS+
Sbjct: 126 PSGNMSASVMNGFEPDKVPVYKSLVSEFAVFDRWFASVPSSTQPNRMFVHSGTSAGATSN 185

Query: 186 LKKQLAVGYPQKTIFDSLHENGLDFGIYFQNIPTTLFYRNLRKLKYIFKFHDYDLKFKKD 245
               LA GYPQ+TIFD+L +    FGIY+QNIP  LFY++LRKLKY+FKFH Y   FK  
Sbjct: 186 NPISLAKGYPQRTIFDNLDDEEFSFGIYYQNIPAVLFYQSLRKLKYVFKFHSYGNSFKDH 245

Query: 246 ATNGKLPSLTVIEPRYFDLVGMPANDDHPSHDVANGQKLVKEVYETLRASPQWNQTLLII 305
           A  GKLP+ TVIE RY D +  PA+DDHPSHDV  GQK +KEVYETLRASPQWN+TLLII
Sbjct: 246 AKQGKLPAYTVIEQRYMDTLLEPASDDHPSHDVYQGQKFIKEVYETLRASPQWNETLLII 305

Query: 306 TYDEH--------------------------------------------------VISSP 365
           TYDEH                                                  V+  P
Sbjct: 306 TYDEHGGYFDHVPTPVRNVPSPDGIVGPDPFLFQFNRLGIRVPTIAVSPWIEKGTVVHGP 365

Query: 366 KG-PTPNSEFELSSIPATIKKIFNLSSNFLTHRDAWAGTFEDIVGQLTSPRTDCPVTLPE 425
            G P P+SE+E SSIPAT+KK+FNLSS FLT RD WAGTFE+I+     PRTDCP TLPE
Sbjct: 366 NGSPFPSSEYEHSSIPATVKKLFNLSSPFLTKRDEWAGTFENILQIRKEPRTDCPETLPE 425

Query: 426 VTALRKTEANENSAISEFQSEVVQLGAVLNGDHFLSSFPDEISKKMSVKEAHEYTKGAVS 458
              +R  EANE + ++EFQ E+VQL AVL GD+ L++FP EISK M+V E   Y + A+ 
Sbjct: 426 PVKIRMGEANEKALLTEFQQELVQLAAVLKGDNMLTTFPKEISKGMTVIEGKRYMEDAMK 485

BLAST of Sed0002234 vs. TAIR 10
Match: AT3G03520.1 (non-specific phospholipase C3 )

HSP 1 Score: 435.3 bits (1118), Expect = 6.3e-122
Identity = 232/497 (46.68%), Postives = 308/497 (61.97%), Query Frame = 0

Query: 26  PIQTVVVLVLENRSFDHMLGWMKKYMDDSRINGVTGHE--CNPVSTNNPNPQTVCFSDDA 85
           PI+T+VVLV ENRSFDHMLGW K+   +  I+GV+  E   NP+ST++PN   + F  ++
Sbjct: 15  PIKTIVVLVQENRSFDHMLGWFKEL--NPEIDGVSESEPRSNPLSTSDPNSAQIFFGKES 74

Query: 86  EFVDPDPGHSFEAVLHQVFGSG-------SIPSMSGFVEQALSISPNLSQ-AVMKGFKPQ 145
           + +DPDPGHSF+A+  QVFG           P M+GFV+ A +I+  +S+  VM+GF P+
Sbjct: 75  QNIDPDPGHSFQAIYEQVFGKPFSDESPYPDPKMNGFVQNAEAITKGMSEKVVMQGFPPE 134

Query: 146 SLPVYAALVPEFAVFDRWFSSIPGPTQPNRLFVYSATSHGSTSHLKKQLAVGYPQKTIFD 205
            LPV+  LV EFAV DRWFSS+P  TQPNRL+V++ATS+G+ S+    L  G+PQ+T+F+
Sbjct: 135 KLPVFKELVQEFAVCDRWFSSLPSSTQPNRLYVHAATSNGAFSNDTNTLVRGFPQRTVFE 194

Query: 206 SLHENGLDFGIYFQNIPTTLFYRNLRKLKYIFKFHDYDLKFKKDATNGKLPSLTVIEPRY 265
           SL E+G  FGIY+Q+ P  LFYRN+RKLKY+  FH Y L FK+    GKLP+  VIEPRY
Sbjct: 195 SLEESGFTFGIYYQSFPNCLFYRNMRKLKYVDNFHQYHLSFKRHCKEGKLPNYVVIEPRY 254

Query: 266 FDLVGMPANDDHPSHDVANGQKLVKEVYETLRASPQWNQTLLIITYDEH----------- 325
           F ++  PANDDHP +DV  GQ LVKE+YE LRASPQWN+ L ++ YDEH           
Sbjct: 255 FKILSAPANDDHPKNDVVEGQNLVKEIYEALRASPQWNEILFVVVYDEHGGYYDHVPTPV 314

Query: 326 ---------------------------------------VISSPKGPTPNSEFELSSIPA 385
                                                  V+  P GP P S+FE SSIPA
Sbjct: 315 IGVPNPDGLVGPEPYNFKFDRLGVRVPALLISPWIEPGTVLHEPNGPEPTSQFEHSSIPA 374

Query: 386 TIKKIFNLSSNFLTHRDAWAGTFEDIVGQLTSPRTDCPVTLPEVTALRKTE---ANENSA 445
           T+KKIFNL S FLT RD WAGT + ++ + TSPRTDCPVTLPE+   R  +     E+  
Sbjct: 375 TLKKIFNLKS-FLTKRDEWAGTLDAVINR-TSPRTDCPVTLPELPRARDIDIGTQEEDED 434

Query: 446 ISEFQSEVVQLGAVLNGDHFLSSFPDEISKKMSVKEAHEYTKGAVSRFIRASKEAIKLGA 460
           +++FQ E++Q  AVL GDH    +P +++ KM V +A  Y + A +RF   SK+A + G 
Sbjct: 435 LTDFQIELIQAAAVLKGDHIKDIYPFKLADKMKVLDAARYVEEAFTRFHGESKKAKEEGR 494

BLAST of Sed0002234 vs. TAIR 10
Match: AT3G03530.1 (non-specific phospholipase C4 )

HSP 1 Score: 427.9 bits (1099), Expect = 1.0e-119
Identity = 232/485 (47.84%), Postives = 298/485 (61.44%), Query Frame = 0

Query: 26  PIQTVVVLVLENRSFDHMLGWMKKYMDDSRINGVTGHE--CNPVSTNNPNPQTVCFSDDA 85
           PI+T+VVLV ENRSFDH LGW K+   +  I+GVT  +   N VS+++ N   V F D +
Sbjct: 13  PIKTIVVLVQENRSFDHTLGWFKEL--NREIDGVTKSDPKSNTVSSSDTNSLRVVFGDQS 72

Query: 86  EFVDPDPGHSFEAVLHQVFG----------SGSIPSMSGFVEQALSISPNLSQAVMKGFK 145
           ++V+PDPGHS + +  QVFG          +   P+MSGF + A      +S AVM GFK
Sbjct: 73  QYVNPDPGHSIQDIYEQVFGKPWDSGKPDPNPGHPNMSGFAQNAERNKKGMSSAVMNGFK 132

Query: 146 PQSLPVYAALVPEFAVFDRWFSSIPGPTQPNRLFVYSATSHGSTSHLKKQLAVGYPQKTI 205
           P +LPVY  LV  FA+ DRWF+S+P  TQPNRL+V+SATSHG+TS+ KK L  G+PQKTI
Sbjct: 133 PNALPVYKELVQNFAICDRWFASVPASTQPNRLYVHSATSHGATSNDKKLLLEGFPQKTI 192

Query: 206 FDSLHENGLDFGIYFQNIPTTLFYRNLRKLKYIFKFHDYDLKFKKDATNGKLPSLTVIEP 265
           F+SL E G  FGIY+Q  P+TLFYRNLRKLKY+  FH Y ++FKKD   GKLP+  V+E 
Sbjct: 193 FESLDEAGFSFGIYYQFPPSTLFYRNLRKLKYLTHFHQYGIQFKKDCKEGKLPNYVVVEQ 252

Query: 266 RYFDLVGMPANDDHPSHDVANGQKLVKEVYETLRASPQWNQTLLIITYDEH--------- 325
           R+FDL+  PANDDHPSHDV+ GQKLVKEVYE LR+SPQWN+ L IITYDEH         
Sbjct: 253 RWFDLLSTPANDDHPSHDVSEGQKLVKEVYEALRSSPQWNEILFIITYDEHGGFYDHVPT 312

Query: 326 -----------------------------------------VISSPKGPTPNSEFELSSI 385
                                                    VI  P GP P S++E SSI
Sbjct: 313 PVDGVPNPDGILGPPPYNFEFNRLGVRVPTFFISPWIEPGTVIHGPNGPYPRSQYEHSSI 372

Query: 386 PATIKKIFNLSSNFLTHRDAWAGTFEDIVGQLTSPRTDCPVTLPEVTALRKTEANENSAI 445
           PAT+K IF L  +FL+ RD+WAGTFE ++ +  SPR DCP TL     LR T A EN+ +
Sbjct: 373 PATVKTIFKL-KDFLSKRDSWAGTFESVITR-DSPRQDCPETLSTPIKLRGTMAKENAQL 432

Query: 446 SEFQSEVVQLGAVLNGDHFLSSFPDEISKKMSVKEAHEYTKGAVSRFIRASKEAIKLGAD 449
           SEFQ ++V + A L GD+       ++ K+  V +A +Y   A  +F+  S++A   G D
Sbjct: 433 SEFQEDLVIMAAGLKGDYKNEELIHKLCKETCVADASKYVTNAFEKFLEESRKARDRGCD 492

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022921419.12.0e-23181.10non-specific phospholipase C6-like [Cucurbita moschata][more]
XP_023515458.13.5e-23181.10non-specific phospholipase C6-like [Cucurbita pepo subsp. pepo][more]
XP_022987132.11.3e-23080.91non-specific phospholipase C6-like [Cucurbita maxima][more]
KAG6589378.16.5e-23080.91Non-specific phospholipase C6, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_023531289.11.1e-22980.91non-specific phospholipase C6-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Q8H9652.2e-19669.74Non-specific phospholipase C6 OS=Arabidopsis thaliana OX=3702 GN=NPC6 PE=2 SV=1[more]
Q8L7Y93.1e-14252.73Non-specific phospholipase C1 OS=Arabidopsis thaliana OX=3702 GN=NPC1 PE=2 SV=1[more]
O810201.7e-14052.06Non-specific phospholipase C2 OS=Arabidopsis thaliana OX=3702 GN=NPC2 PE=2 SV=1[more]
Q9SRQ68.9e-12146.68Non-specific phospholipase C3 OS=Arabidopsis thaliana OX=3702 GN=NPC3 PE=1 SV=1[more]
Q9SRQ71.4e-11847.84Non-specific phospholipase C4 OS=Arabidopsis thaliana OX=3702 GN=NPC4 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1E0F19.8e-23281.10non-specific phospholipase C6-like OS=Cucurbita moschata OX=3662 GN=LOC111429704... [more]
A0A6J1J9I66.4e-23180.91non-specific phospholipase C6-like OS=Cucurbita maxima OX=3661 GN=LOC111484775 P... [more]
A0A6J1L5I11.2e-22980.52non-specific phospholipase C6-like OS=Cucurbita maxima OX=3661 GN=LOC111500200 P... [more]
A0A6J1EP582.7e-22980.52non-specific phospholipase C6-like isoform X1 OS=Cucurbita moschata OX=3662 GN=L... [more]
A0A5D3BH493.5e-22980.24Non-specific phospholipase C6 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sc... [more]
Match NameE-valueIdentityDescription
AT3G48610.11.6e-19769.74non-specific phospholipase C6 [more]
AT1G07230.12.2e-14352.73non-specific phospholipase C1 [more]
AT2G26870.11.2e-14152.06non-specific phospholipase C2 [more]
AT3G03520.16.3e-12246.68non-specific phospholipase C3 [more]
AT3G03530.11.0e-11947.84non-specific phospholipase C4 [more]
InterPro
Analysis Name: InterPro Annotations of Chayote (edule) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR007312PhosphoesterasePFAMPF04185Phosphoesterasecoord: 27..304
e-value: 5.1E-77
score: 259.4
IPR007312PhosphoesterasePANTHERPTHR31956NON-SPECIFIC PHOSPHOLIPASE C4-RELATEDcoord: 305..461
coord: 5..304
IPR017850Alkaline-phosphatase-like, core domain superfamilyGENE3D3.40.720.10Alkaline Phosphatase, subunit Acoord: 20..186
e-value: 3.9E-31
score: 110.8
coord: 236..382
e-value: 1.1E-22
score: 83.2
NoneNo IPR availablePANTHERPTHR31956:SF35SUBFAMILY NOT NAMEDcoord: 5..304
coord: 305..461

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sed0002234.1Sed0002234.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0048229 gametophyte development
biological_process GO:0009395 phospholipid catabolic process
biological_process GO:0048364 root development
cellular_component GO:0009507 chloroplast
molecular_function GO:0004629 phospholipase C activity
molecular_function GO:0003824 catalytic activity
molecular_function GO:0016788 hydrolase activity, acting on ester bonds