Sed0001812 (gene) Chayote v1

BLAST of Sed0001812 vs. NCBI nr
Match: XP_038905601.1 (GDSL esterase/lipase At5g14450-like isoform X1 [Benincasa hispida])

HSP 1 Score: 673.7 bits (1737), Expect = 9.1e-190
Identity = 324/373 (86.86%), Postives = 349/373 (93.57%), Query Frame = 0

Query: 1   MKIQMSLMVSSILISMADSKACNFPAIYNFGDSNSDTGGISAAFYPTILPTGETFFHKTA 60
           MKIQM+L V+SILISMADS+AC+FPAIYNFGDSNSDTGGISAAFYPTILP GETFFHKTA
Sbjct: 5   MKIQMALTVASILISMADSRACDFPAIYNFGDSNSDTGGISAAFYPTILPCGETFFHKTA 64

Query: 61  GRGCDGRLILDFIAKHLELPYLSAYLNSIGANFRHGANFATGGSTIRRQNESVFENGVSP 120
           GRGCDGRLI+DFIAKHLELPYLSAYLNSIG NFRHGANFATGGSTIRRQNESVFENGVSP
Sbjct: 65  GRGCDGRLIIDFIAKHLELPYLSAYLNSIGTNFRHGANFATGGSTIRRQNESVFENGVSP 124

Query: 121 FSLDIQIVQFRQFKNRTIDRYLEATKEDSIRSTLPIPEDFSKALFTIDIGQNDLSVGFRL 180
           FSLDIQIVQFRQFKNRTIDRYLEA  +DSI++TLPIPE+FSKA+FTIDIGQNDLS GFR 
Sbjct: 125 FSLDIQIVQFRQFKNRTIDRYLEAV-DDSIKNTLPIPEEFSKAIFTIDIGQNDLSAGFR- 184

Query: 181 NFTDHQFRVAIPNIIAEFSAAIEELYKEGARAFWVHNTGPIGCIPVAIRSVSSPVTGDFD 240
             T+ QF   IPNII EFS AIE+LYKEGARAFWVHNTGPIGCIPVAIRS+ +P+ GDFD
Sbjct: 185 KMTNAQFLTTIPNIIDEFSRAIEDLYKEGARAFWVHNTGPIGCIPVAIRSIPNPIPGDFD 244

Query: 241 QNGCVRDQNDAALEFNRQLKEQVIKLRPKLSNASLVYVDVFAAKIKLIANAKEQGFMEKG 300
           QNGCVR QNDAALEFN+QLKE++IKLR  LS+ASLVYVDVFAAK+KLIANAKE+GFMEKG
Sbjct: 245 QNGCVRYQNDAALEFNKQLKERIIKLRTILSDASLVYVDVFAAKLKLIANAKEEGFMEKG 304

Query: 301 AICCGYHEGNNHVWCGNKKIINGSEVYAGSCDDPSKLISWDGVHYTEAANRWIADQILNG 360
            ICCGYHEGNNHVWCGN+KIINGSEVY GSCDDPSK ISWDGVHYTEAANRWIA+QI+ G
Sbjct: 305 EICCGYHEGNNHVWCGNRKIINGSEVYGGSCDDPSKFISWDGVHYTEAANRWIANQIVKG 364

Query: 361 SFSDPQVPITHAC 374
           SFSDPQ+PITHAC
Sbjct: 365 SFSDPQIPITHAC 375

BLAST of Sed0001812 vs. NCBI nr
Match: XP_038905602.1 (GDSL esterase/lipase At5g14450-like isoform X2 [Benincasa hispida])

HSP 1 Score: 671.4 bits (1731), Expect = 4.5e-189
Identity = 323/373 (86.60%), Postives = 348/373 (93.30%), Query Frame = 0

Query: 1   MKIQMSLMVSSILISMADSKACNFPAIYNFGDSNSDTGGISAAFYPTILPTGETFFHKTA 60
           MKIQM+L V+SILISMADS+AC+FPAIYNFGDSNSDTGGISAAFYPTILP GETFFHKTA
Sbjct: 5   MKIQMALTVASILISMADSRACDFPAIYNFGDSNSDTGGISAAFYPTILPCGETFFHKTA 64

Query: 61  GRGCDGRLILDFIAKHLELPYLSAYLNSIGANFRHGANFATGGSTIRRQNESVFENGVSP 120
           GRGCDGRLI+DFIAKHLELPYLSAYLNSIG NFRHGANFATGGSTIRRQNESVFENGVSP
Sbjct: 65  GRGCDGRLIIDFIAKHLELPYLSAYLNSIGTNFRHGANFATGGSTIRRQNESVFENGVSP 124

Query: 121 FSLDIQIVQFRQFKNRTIDRYLEATKEDSIRSTLPIPEDFSKALFTIDIGQNDLSVGFRL 180
           FSLDIQIVQFRQFKNRTIDRYLE   +DSI++TLPIPE+FSKA+FTIDIGQNDLS GFR 
Sbjct: 125 FSLDIQIVQFRQFKNRTIDRYLEV--DDSIKNTLPIPEEFSKAIFTIDIGQNDLSAGFR- 184

Query: 181 NFTDHQFRVAIPNIIAEFSAAIEELYKEGARAFWVHNTGPIGCIPVAIRSVSSPVTGDFD 240
             T+ QF   IPNII EFS AIE+LYKEGARAFWVHNTGPIGCIPVAIRS+ +P+ GDFD
Sbjct: 185 KMTNAQFLTTIPNIIDEFSRAIEDLYKEGARAFWVHNTGPIGCIPVAIRSIPNPIPGDFD 244

Query: 241 QNGCVRDQNDAALEFNRQLKEQVIKLRPKLSNASLVYVDVFAAKIKLIANAKEQGFMEKG 300
           QNGCVR QNDAALEFN+QLKE++IKLR  LS+ASLVYVDVFAAK+KLIANAKE+GFMEKG
Sbjct: 245 QNGCVRYQNDAALEFNKQLKERIIKLRTILSDASLVYVDVFAAKLKLIANAKEEGFMEKG 304

Query: 301 AICCGYHEGNNHVWCGNKKIINGSEVYAGSCDDPSKLISWDGVHYTEAANRWIADQILNG 360
            ICCGYHEGNNHVWCGN+KIINGSEVY GSCDDPSK ISWDGVHYTEAANRWIA+QI+ G
Sbjct: 305 EICCGYHEGNNHVWCGNRKIINGSEVYGGSCDDPSKFISWDGVHYTEAANRWIANQIVKG 364

Query: 361 SFSDPQVPITHAC 374
           SFSDPQ+PITHAC
Sbjct: 365 SFSDPQIPITHAC 374

BLAST of Sed0001812 vs. NCBI nr
Match: XP_004141199.2 (GDSL esterase/lipase At5g14450 [Cucumis sativus] >KGN59717.1 hypothetical protein Csa_001756 [Cucumis sativus])

HSP 1 Score: 652.5 bits (1682), Expect = 2.2e-183
Identity = 315/374 (84.22%), Postives = 346/374 (92.51%), Query Frame = 0

Query: 1   MKIQMSLMVSS-ILISMADSKACNFPAIYNFGDSNSDTGGISAAFYPTILPTGETFFHKT 60
           MK++M   V+S ILISMADS+ACNFPAIYNFGDSNSDTGGISAAFYPTILP G+TFFHKT
Sbjct: 7   MKMKMVFSVASIILISMADSRACNFPAIYNFGDSNSDTGGISAAFYPTILPCGQTFFHKT 66

Query: 61  AGRGCDGRLILDFIAKHLELPYLSAYLNSIGANFRHGANFATGGSTIRRQNESVFENGVS 120
           AGRGCDGRLI+DFIAK LELPYLSAYLNSIG NFRHGANFATGGSTIRRQNESVFENG+S
Sbjct: 67  AGRGCDGRLIIDFIAKQLELPYLSAYLNSIGTNFRHGANFATGGSTIRRQNESVFENGIS 126

Query: 121 PFSLDIQIVQFRQFKNRTIDRYLEATKEDSIRSTLPIPEDFSKALFTIDIGQNDLSVGFR 180
           PFSLDIQ+VQFRQFKNRTIDRY+EA  +DSIRSTLP+PE+FSKALFTIDIGQNDLS GFR
Sbjct: 127 PFSLDIQVVQFRQFKNRTIDRYVEAI-DDSIRSTLPVPEEFSKALFTIDIGQNDLSAGFR 186

Query: 181 LNFTDHQFRVAIPNIIAEFSAAIEELYKEGARAFWVHNTGPIGCIPVAIRSVSSPVTGDF 240
              T+ QFR AIP+II EF+ A+E+LYKEGARAFWVHNTGPIGCIPVAIRSVS+P  GD 
Sbjct: 187 -KMTNDQFRKAIPDIINEFATAVEDLYKEGARAFWVHNTGPIGCIPVAIRSVSNPKEGDL 246

Query: 241 DQNGCVRDQNDAALEFNRQLKEQVIKLRPKLSNASLVYVDVFAAKIKLIANAKEQGFMEK 300
           D+NGCV++QNDAALEFNRQLKE+V+KLR  L +ASLVYVDV+AAKIKLIANAKE+GFMEK
Sbjct: 247 DRNGCVKEQNDAALEFNRQLKERVVKLRANLLDASLVYVDVYAAKIKLIANAKEEGFMEK 306

Query: 301 GAICCGYHEGNNHVWCGNKKIINGSEVYAGSCDDPSKLISWDGVHYTEAANRWIADQILN 360
           GAICCGYHEG NHVWCGN+K INGSEVYAGSC+DPSK ISWDGVHYTEAAN+WIA+QI+ 
Sbjct: 307 GAICCGYHEGLNHVWCGNRKTINGSEVYAGSCEDPSKFISWDGVHYTEAANQWIANQIVK 366

Query: 361 GSFSDPQVPITHAC 374
           GSFSDPQVPI HAC
Sbjct: 367 GSFSDPQVPIMHAC 378

BLAST of Sed0001812 vs. NCBI nr
Match: KAG7017790.1 (GDSL esterase/lipase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 651.7 bits (1680), Expect = 3.7e-183
Identity = 319/374 (85.29%), Postives = 346/374 (92.51%), Query Frame = 0

Query: 1   MKIQMSLM-VSSILISMADSKACNFPAIYNFGDSNSDTGGISAAFYPTILPTGETFFHKT 60
           MKI M+   V+SILISMADS+AC+FPAIYNFGDSNSDTGGISAAFYPTILP GETFFHKT
Sbjct: 1   MKIPMAFFTVASILISMADSRACDFPAIYNFGDSNSDTGGISAAFYPTILPCGETFFHKT 60

Query: 61  AGRGCDGRLILDFIAKHLELPYLSAYLNSIGANFRHGANFATGGSTIRRQNESVFENGVS 120
           AGRGCDGRLI+DFIAKHLELPYL+AYLNSIGANFRHGANFATGGSTIR QNESVFENGVS
Sbjct: 61  AGRGCDGRLIIDFIAKHLELPYLTAYLNSIGANFRHGANFATGGSTIRNQNESVFENGVS 120

Query: 121 PFSLDIQIVQFRQFKNRTIDRYLEATKEDSIRSTLPIPEDFSKALFTIDIGQNDLSVGFR 180
           PFSLDIQ++QFRQFKNRTIDRY E T  D IRSTLPIPEDFSKALFTIDIGQNDLS GFR
Sbjct: 121 PFSLDIQVIQFRQFKNRTIDRYREET-NDFIRSTLPIPEDFSKALFTIDIGQNDLSAGFR 180

Query: 181 LNFTDHQFRVAIPNIIAEFSAAIEELYKEGARAFWVHNTGPIGCIPVAIRSVSSPVTGDF 240
              T+ QFR+AIPNII EF+AAIEELY+EGARAFWVHNTGPIGCIPVAIR+    V GD 
Sbjct: 181 -KMTNDQFRMAIPNIIGEFAAAIEELYREGARAFWVHNTGPIGCIPVAIRTT---VHGDL 240

Query: 241 DQNGCVRDQNDAALEFNRQLKEQVIKLRPKLSNASLVYVDVFAAKIKLIANAKEQGFMEK 300
           DQNGCV+ QNDAALEFNRQLKE+VI+LR KLS+ASLV+VDVFAAK++LIANAKE+GFME 
Sbjct: 241 DQNGCVKYQNDAALEFNRQLKERVIQLRAKLSDASLVHVDVFAAKLQLIANAKEEGFMEM 300

Query: 301 GAICCGYHEGNNHVWCGNKKIINGSEVYAGSCDDPSKLISWDGVHYTEAANRWIADQILN 360
           GAICCGYHEGN+HVWCGN+KIINGS+VYAGSCDDPS+ ISWDGVHYTEAANRWIA+QI+ 
Sbjct: 301 GAICCGYHEGNDHVWCGNQKIINGSQVYAGSCDDPSRFISWDGVHYTEAANRWIANQIIK 360

Query: 361 GSFSDPQVPITHAC 374
           GSFSDPQVPITHAC
Sbjct: 361 GSFSDPQVPITHAC 369

BLAST of Sed0001812 vs. NCBI nr
Match: XP_022982674.1 (GDSL esterase/lipase At5g14450-like [Cucurbita maxima])

HSP 1 Score: 649.0 bits (1673), Expect = 2.4e-182
Identity = 317/374 (84.76%), Postives = 344/374 (91.98%), Query Frame = 0

Query: 1   MKIQMSLM-VSSILISMADSKACNFPAIYNFGDSNSDTGGISAAFYPTILPTGETFFHKT 60
           MKI M+   V+SILISMADS+AC FPAIYNFGDSNSDTGGISAAFYPTILP GETFFHKT
Sbjct: 3   MKIPMAFFTVASILISMADSRACGFPAIYNFGDSNSDTGGISAAFYPTILPCGETFFHKT 62

Query: 61  AGRGCDGRLILDFIAKHLELPYLSAYLNSIGANFRHGANFATGGSTIRRQNESVFENGVS 120
           AGRGCDGRLI+DF+AKHLELPYLSAYLNSIGANFRHGANFATGGSTIR QNESVFENGVS
Sbjct: 63  AGRGCDGRLIIDFLAKHLELPYLSAYLNSIGANFRHGANFATGGSTIRNQNESVFENGVS 122

Query: 121 PFSLDIQIVQFRQFKNRTIDRYLEATKEDSIRSTLPIPEDFSKALFTIDIGQNDLSVGFR 180
           PFSLDIQ+VQFRQFKNRTIDRY E T  D IRSTLP+PE+FS ALFTIDIGQNDLS GFR
Sbjct: 123 PFSLDIQVVQFRQFKNRTIDRYHEET-NDFIRSTLPVPEEFSNALFTIDIGQNDLSAGFR 182

Query: 181 LNFTDHQFRVAIPNIIAEFSAAIEELYKEGARAFWVHNTGPIGCIPVAIRSVSSPVTGDF 240
              T++QF++AIPNII EF AAIEELY+EGARAFWVHNTGPIGCIPVAIR+    V GD 
Sbjct: 183 -KMTNYQFQMAIPNIIGEFDAAIEELYREGARAFWVHNTGPIGCIPVAIRTT---VHGDL 242

Query: 241 DQNGCVRDQNDAALEFNRQLKEQVIKLRPKLSNASLVYVDVFAAKIKLIANAKEQGFMEK 300
           DQNGCV+ QNDAALEFNRQLKE+VI+LR KLS+ASLVYVDVFAAK++LIANAKE+GFME 
Sbjct: 243 DQNGCVKYQNDAALEFNRQLKERVIQLRAKLSDASLVYVDVFAAKLQLIANAKEEGFMEM 302

Query: 301 GAICCGYHEGNNHVWCGNKKIINGSEVYAGSCDDPSKLISWDGVHYTEAANRWIADQILN 360
           GAICCGYHEGN+HVWCGN+KIINGS+VYAGSCDDPS+ ISWDGVHYTEAANRWIA+QI+ 
Sbjct: 303 GAICCGYHEGNDHVWCGNQKIINGSQVYAGSCDDPSRFISWDGVHYTEAANRWIANQIIK 362

Query: 361 GSFSDPQVPITHAC 374
           GSFSDPQVPITHAC
Sbjct: 363 GSFSDPQVPITHAC 371

BLAST of Sed0001812 vs. ExPASy Swiss-Prot
Match: Q9LY84 (GDSL esterase/lipase At5g14450 OS=Arabidopsis thaliana OX=3702 GN=At5g14450 PE=2 SV=1)

HSP 1 Score: 443.7 bits (1140), Expect = 2.0e-123
Identity = 207/367 (56.40%), Postives = 270/367 (73.57%), Query Frame = 0

Query: 7   LMVSSILISMADSKACNFPAIYNFGDSNSDTGGISAAFYPTILPTGETFFHKTAGRGCDG 66
           L + ++  S++    C FPAIYNFGDSNSDTGGISAAF P   P G+ FFH+  GR  DG
Sbjct: 22  LCLFAVTTSVSVQPTCTFPAIYNFGDSNSDTGGISAAFEPIRDPYGQGFFHRPTGRDSDG 81

Query: 67  RLILDFIAKHLELPYLSAYLNSIGANFRHGANFATGGSTIRRQNESVFENGVSPFSLDIQ 126
           RL +DFIA+ L LPYLSAYLNS+G+NFRHGANFATGGSTIRRQNE++F+ G+SPFSLD+Q
Sbjct: 82  RLTIDFIAERLGLPYLSAYLNSLGSNFRHGANFATGGSTIRRQNETIFQYGISPFSLDMQ 141

Query: 127 IVQFRQFKNRTIDRYLEATKEDSIRSTLPIPEDFSKALFTIDIGQNDLSVGFRLNFTDHQ 186
           I QF QFK R+   + +  K    R  LP  E+F+KAL+T DIGQNDLSVGFR    D Q
Sbjct: 142 IAQFDQFKARSALLFTQ-IKSRYDREKLPRQEEFAKALYTFDIGQNDLSVGFRTMSVD-Q 201

Query: 187 FRVAIPNIIAEFSAAIEELYKEGARAFWVHNTGPIGCIPVAIRSVSSPVTGDFDQNGCVR 246
            +  IP+I+   ++A+  +Y++G R FWVHNTGP GC+PV +  + +P  G  D++GCV+
Sbjct: 202 LKATIPDIVNHLASAVRNIYQQGGRTFWVHNTGPFGCLPVNMFYMGTPAPGYLDKSGCVK 261

Query: 247 DQNDAALEFNRQLKEQVIKLRPKLSNASLVYVDVFAAKIKLIANAKEQGFMEKGAICCGY 306
            QN+ A+EFNR+LKE VI LR +L+ A++ YVDV+ AK ++++N K+ GF     +CCGY
Sbjct: 262 AQNEMAMEFNRKLKETVINLRKELTQAAITYVDVYTAKYEMMSNPKKLGFANPLKVCCGY 321

Query: 307 HEGNNHVWCGNKKIINGSEVYAGSCDDPSKLISWDGVHYTEAANRWIADQILNGSFSDPQ 366
           HE  +H+WCGNK  +N +E+Y GSC +P   +SWDGVHYTEAAN+ +AD+ LNG  +DP 
Sbjct: 322 HEKYDHIWCGNKGKVNNTEIYGGSCPNPVMAVSWDGVHYTEAANKHVADRTLNGLLTDPP 381

Query: 367 VPITHAC 374
           VPIT AC
Sbjct: 382 VPITRAC 386

BLAST of Sed0001812 vs. ExPASy Swiss-Prot
Match: Q9LIN2 (GDSL esterase/lipase At3g26430 OS=Arabidopsis thaliana OX=3702 GN=At3g26430 PE=1 SV=1)

HSP 1 Score: 361.3 bits (926), Expect = 1.3e-98
Identity = 177/375 (47.20%), Postives = 246/375 (65.60%), Query Frame = 0

Query: 3   IQMSLMVSSILISMADSKACNFPAIYNFGDSNSDTGGISAAFYPTILPTGETFFHKTAGR 62
           ++  L+ S ++   A S +CNFPAI+NFGDSNSDTGG+SA+F     P G+TFFH  +GR
Sbjct: 8   VKCVLLASCLIHPRACSPSCNFPAIFNFGDSNSDTGGLSASFGQAPYPNGQTFFHSPSGR 67

Query: 63  GCDGRLILDFIAKHLELPYLSAYLNSIGANFRHGANFATGGSTIRRQNESVFENGVSPFS 122
             DGRLI+DFIA+ L LPYL+A+L+SIG+NF HGANFAT GST+R  N ++ ++GVSP S
Sbjct: 68  FSDGRLIIDFIAEELGLPYLNAFLDSIGSNFSHGANFATAGSTVRPPNATIAQSGVSPIS 127

Query: 123 LDIQIVQFRQFKNRTIDRYLEATKEDSIRSTLPIPEDFSKALFTIDIGQNDLSVGFRLNF 182
           LD+Q+VQF  F  R+    L   +    +  LP  E FS+AL+T DIGQNDL+ G +LN 
Sbjct: 128 LDVQLVQFSDFITRS---QLIRNRGGVFKKLLPKKEYFSQALYTFDIGQNDLTAGLKLNM 187

Query: 183 TDHQFRVAIPNIIAEFSAAIEELYKEGARAFWVHNTGPIGCIPVAIRSVSSPVTGDFDQN 242
           T  Q +  IP++  + S  I ++Y +G R FW+HNT P+GC+P  +     P +   D +
Sbjct: 188 TSDQIKAYIPDVHDQLSNVIRKVYSKGGRRFWIHNTAPLGCLPYVLDRFPVPAS-QIDNH 247

Query: 243 GCVRDQNDAALEFNRQLKEQVIKLRPKLSNASLVYVDVFAAKIKLIANAKEQGFMEKGAI 302
           GC   +N+ A  +N +LK +VI+LR +LS A+  YVD+++ K+ LI  AK+ GF      
Sbjct: 248 GCAIPRNEIARYYNSELKRRVIELRKELSEAAFTYVDIYSIKLTLITQAKKLGFRYPLVA 307

Query: 303 CCGY---HEGNNHVWCGNKKIINGSE-VYAGSCDDPSKLISWDGVHYTEAANRWIADQIL 362
           CCG+   +  N  + CG K +I G E V A SC+D S  +SWDG+H+TE  N WI  QI 
Sbjct: 308 CCGHGGKYNFNKLIKCGAKVMIKGKEIVLAKSCNDVSFRVSWDGIHFTETTNSWIFQQIN 367

Query: 363 NGSFSDPQVPITHAC 374
           +G+FSDP +P+  AC
Sbjct: 368 DGAFSDPPLPVKSAC 378

BLAST of Sed0001812 vs. ExPASy Swiss-Prot
Match: Q9FXE5 (Alpha-L-fucosidase 3 OS=Arabidopsis thaliana OX=3702 GN=FXG1 PE=2 SV=1)

HSP 1 Score: 350.1 bits (897), Expect = 3.0e-95
Identity = 179/365 (49.04%), Postives = 239/365 (65.48%), Query Frame = 0

Query: 3   IQMSLMVSSILISMADS---KACNFPAIYNFGDSNSDTGGISAAFYPTILPTGETFFHKT 62
           I  SL   S+L S++ S     C+FPAI+NFGDSNSDTGG+SAAF     P G +FF   
Sbjct: 4   ILSSLFALSLLSSLSPSTHAHQCHFPAIFNFGDSNSDTGGLSAAFGQAGPPHGSSFFGSP 63

Query: 63  AGRGCDGRLILDFIAKHLELPYLSAYLNSIGANFRHGANFATGGSTIRRQNESVFENGVS 122
           AGR CDGRL++DFIA+ L LPYLSA+L+S+G+NF HGANFAT GS IR  N ++ ++G S
Sbjct: 64  AGRYCDGRLVIDFIAESLGLPYLSAFLDSVGSNFSHGANFATAGSPIRALNSTLRQSGFS 123

Query: 123 PFSLDIQIVQFRQFKNRTIDRYLEATKEDSIRSTLPIPEDFSKALFTIDIGQNDLSVGFR 182
           PFSLD+Q VQF  F NR+       ++    ++ LP  + FSKAL+T DIGQNDL+ G+ 
Sbjct: 124 PFSLDVQFVQFYNFHNRS---QTVRSRGGVYKTMLPESDSFSKALYTFDIGQNDLTAGYF 183

Query: 183 LNFTDHQFRVAIPNIIAEFSAAIEELYKEGARAFWVHNTGPIGCIPVAIRSVSSPVTGDF 242
            N T  Q    +P II++F  AI+ +Y +G R FW+HNTGPIGC+   I    +  + DF
Sbjct: 184 ANKTVEQVETEVPEIISQFMNAIKNIYGQGGRYFWIHNTGPIGCLAYVIERFPNKAS-DF 243

Query: 243 DQNGCVRDQNDAALEFNRQLKEQVIKLRPKLSNASLVYVDVFAAKIKLIANAKEQGFMEK 302
           D +GCV   N  A +FN  LK+ VI+LR  LS A++ YVDV++ K +L  +A+  GF   
Sbjct: 244 DSHGCVSPLNHLAQQFNHALKQAVIELRSSLSEAAITYVDVYSLKHELFVHAQGHGFKGS 303

Query: 303 GAICCGY---HEGNNHVWCGNKKIINGSEVYAGS-CDDPSKLISWDGVHYTEAANRWIAD 361
              CCG+   +  N  + CG KKI+ G EVY G  CD+P K + WDGVH+T+AAN++I D
Sbjct: 304 LVSCCGHGGKYNYNKGIGCGMKKIVKGKEVYIGKPCDEPDKAVVWDGVHFTQAANKFIFD 363

BLAST of Sed0001812 vs. ExPASy Swiss-Prot
Match: B4FZ87 (GDSL esterase/lipase ACHE OS=Zea mays OX=4577 GN=ACHE PE=1 SV=1)

HSP 1 Score: 345.9 bits (886), Expect = 5.7e-94
Identity = 176/360 (48.89%), Postives = 234/360 (65.00%), Query Frame = 0

Query: 22  CNFPAIYNFGDSNSDTGGISAAFYPTILPTGETFFHKTAGRGCDGRLILDFIAKHLELPY 81
           C+FPA++NFGDSNSDTGG+S+ F     P G TFF   AGR CDGRL++DFIA+ L L +
Sbjct: 38  CHFPAVFNFGDSNSDTGGLSSLFGAAPPPNGRTFFGMPAGRYCDGRLVIDFIAESLGLTH 97

Query: 82  LSAYLNSIGANFRHGANFATGGSTIRRQNESVFENGVSPFSLDIQIVQFRQFKNRTIDRY 141
           LSAYLNSIG+NF  GANFAT GS+IRRQN S+F +G SP SLD+Q  +F QF NR+  + 
Sbjct: 98  LSAYLNSIGSNFTQGANFATAGSSIRRQNTSLFLSGFSPISLDVQFWEFEQFINRS--QL 157

Query: 142 LEATKEDSIRSTLPIPEDFSKALFTIDIGQNDLSVGFRLNFTDHQFRVAIPNIIAEFSAA 201
           +   K    R  LP  E FS+AL+T DIGQND++  + +N T  +    IP+++   ++ 
Sbjct: 158 VYNNKGGIYREILPRAEYFSQALYTFDIGQNDITSSYFVNNTTEEVEAIIPDLMERLTSI 217

Query: 202 IEELYKEGARAFWVHNTGPIGCIPVAI---RSVSSPVTGDFDQNGCVRDQNDAALEFNRQ 261
           I+ +Y  G R FW+HNTGP+GC+P A+     ++ P  G     GC    N  A  FN +
Sbjct: 218 IQSVYSRGGRYFWIHNTGPLGCLPYALLHRPDLAIPADG----TGCSVTYNKVAQLFNLR 277

Query: 262 LKEQVIKLRPKLSNASLVYVDVFAAKIKLIANAKEQGFMEKGAICCGYHEGNNH----VW 321
           LKE V  LR    +A+  YVDV+ AK KLI+ A + GF +    CCGY  G  +    V 
Sbjct: 278 LKETVASLRKTHPDAAFTYVDVYTAKYKLISQANKLGFDDPLLTCCGYGGGRYNLDLSVG 337

Query: 322 CGNKKIINGSEVYAG-SCDDPSKLISWDGVHYTEAANRWIADQILNGSFSDPQVPITHAC 374
           CG KK +NG+ V  G SC++PSK +SWDGVH+TEAAN+++ DQI+ G+ SDP V +  AC
Sbjct: 338 CGGKKQVNGTSVVVGKSCENPSKRVSWDGVHFTEAANKFVFDQIVAGALSDPPVALRQAC 391

BLAST of Sed0001812 vs. ExPASy Swiss-Prot
Match: Q9LII9 (GDSL esterase/lipase At3g27950 OS=Arabidopsis thaliana OX=3702 GN=At3g27950 PE=2 SV=1)

HSP 1 Score: 334.0 bits (855), Expect = 2.2e-90
Identity = 170/355 (47.89%), Postives = 231/355 (65.07%), Query Frame = 0

Query: 19  SKACNFPAIYNFGDSNSDTGGISAAFYPTILPTGETFFHKTAGRGCDGRLILDFIAKHLE 78
           S +CNFPA++NFGDSNSDTG ISAA      P G  FF ++AGR  DGRLI+DFI ++L 
Sbjct: 25  SSSCNFPAVFNFGDSNSDTGAISAAIGEVPPPNGVAFFGRSAGRHSDGRLIIDFITENLT 84

Query: 79  LPYLSAYLNSIGANFRHGANFATGGSTIRRQNESVFENGVSPFSLDIQIVQFRQFKNRTI 138
           LPYL+ YL+S+GAN+RHGANFATGGS IR           SPF L  Q+ QF  FK RT+
Sbjct: 85  LPYLTPYLDSVGANYRHGANFATGGSCIRPTLAC-----FSPFHLGTQVSQFIHFKTRTL 144

Query: 139 DRYLEATKEDSIRSTLPIPEDFSKALFTIDIGQNDLSVGFRLNFTDHQFRVAIPNIIAEF 198
             Y    + +   + L     FSKAL+T+DIGQNDL++GF+ N T+ Q +  IP II  F
Sbjct: 145 SLY---NQTNGKFNRLSHTNYFSKALYTLDIGQNDLAIGFQ-NMTEEQLKATIPLIIENF 204

Query: 199 SAAIEELYKEGARAFWVHNTGPIGCIPVAIRSVSSPVTGDFDQNGCVRDQNDAALEFNRQ 258
           + A++ LYKEGAR F +HNTGP GC+P  +++  +      D  GC++  N+ A+EFN+Q
Sbjct: 205 TIALKLLYKEGARFFSIHNTGPTGCLPYLLKAFPAIPR---DPYGCLKPLNNVAIEFNKQ 264

Query: 259 LKEQVIKLRPKLSNASLVYVDVFAAKIKLIANAKEQGFMEKGAICCGYHEGNNHVWCGNK 318
           LK ++ +L+ +L ++   YVDV++AK  LI  AK  GF++    CC    G   + CG  
Sbjct: 265 LKNKITQLKKELPSSFFTYVDVYSAKYNLITKAKALGFIDPFDYCCVGAIGRG-MGCGKT 324

Query: 319 KIINGSEVYAGSCDDPSKLISWDGVHYTEAANRWIADQILNGSFSDPQVPITHAC 374
             +NG+E+Y+ SC +    ISWDG+HYTE AN  +A++IL+GS SDP +P   AC
Sbjct: 325 IFLNGTELYSSSCQNRKNFISWDGIHYTETANMLVANRILDGSISDPPLPTQKAC 366

BLAST of Sed0001812 vs. ExPASy TrEMBL
Match: A0A0A0LG79 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G840430 PE=3 SV=1)

HSP 1 Score: 652.5 bits (1682), Expect = 1.1e-183
Identity = 315/374 (84.22%), Postives = 346/374 (92.51%), Query Frame = 0

Query: 1   MKIQMSLMVSS-ILISMADSKACNFPAIYNFGDSNSDTGGISAAFYPTILPTGETFFHKT 60
           MK++M   V+S ILISMADS+ACNFPAIYNFGDSNSDTGGISAAFYPTILP G+TFFHKT
Sbjct: 7   MKMKMVFSVASIILISMADSRACNFPAIYNFGDSNSDTGGISAAFYPTILPCGQTFFHKT 66

Query: 61  AGRGCDGRLILDFIAKHLELPYLSAYLNSIGANFRHGANFATGGSTIRRQNESVFENGVS 120
           AGRGCDGRLI+DFIAK LELPYLSAYLNSIG NFRHGANFATGGSTIRRQNESVFENG+S
Sbjct: 67  AGRGCDGRLIIDFIAKQLELPYLSAYLNSIGTNFRHGANFATGGSTIRRQNESVFENGIS 126

Query: 121 PFSLDIQIVQFRQFKNRTIDRYLEATKEDSIRSTLPIPEDFSKALFTIDIGQNDLSVGFR 180
           PFSLDIQ+VQFRQFKNRTIDRY+EA  +DSIRSTLP+PE+FSKALFTIDIGQNDLS GFR
Sbjct: 127 PFSLDIQVVQFRQFKNRTIDRYVEAI-DDSIRSTLPVPEEFSKALFTIDIGQNDLSAGFR 186

Query: 181 LNFTDHQFRVAIPNIIAEFSAAIEELYKEGARAFWVHNTGPIGCIPVAIRSVSSPVTGDF 240
              T+ QFR AIP+II EF+ A+E+LYKEGARAFWVHNTGPIGCIPVAIRSVS+P  GD 
Sbjct: 187 -KMTNDQFRKAIPDIINEFATAVEDLYKEGARAFWVHNTGPIGCIPVAIRSVSNPKEGDL 246

Query: 241 DQNGCVRDQNDAALEFNRQLKEQVIKLRPKLSNASLVYVDVFAAKIKLIANAKEQGFMEK 300
           D+NGCV++QNDAALEFNRQLKE+V+KLR  L +ASLVYVDV+AAKIKLIANAKE+GFMEK
Sbjct: 247 DRNGCVKEQNDAALEFNRQLKERVVKLRANLLDASLVYVDVYAAKIKLIANAKEEGFMEK 306

Query: 301 GAICCGYHEGNNHVWCGNKKIINGSEVYAGSCDDPSKLISWDGVHYTEAANRWIADQILN 360
           GAICCGYHEG NHVWCGN+K INGSEVYAGSC+DPSK ISWDGVHYTEAAN+WIA+QI+ 
Sbjct: 307 GAICCGYHEGLNHVWCGNRKTINGSEVYAGSCEDPSKFISWDGVHYTEAANQWIANQIVK 366

Query: 361 GSFSDPQVPITHAC 374
           GSFSDPQVPI HAC
Sbjct: 367 GSFSDPQVPIMHAC 378

BLAST of Sed0001812 vs. ExPASy TrEMBL
Match: A0A6J1J589 (GDSL esterase/lipase At5g14450-like OS=Cucurbita maxima OX=3661 GN=LOC111481479 PE=3 SV=1)

HSP 1 Score: 649.0 bits (1673), Expect = 1.2e-182
Identity = 317/374 (84.76%), Postives = 344/374 (91.98%), Query Frame = 0

Query: 1   MKIQMSLM-VSSILISMADSKACNFPAIYNFGDSNSDTGGISAAFYPTILPTGETFFHKT 60
           MKI M+   V+SILISMADS+AC FPAIYNFGDSNSDTGGISAAFYPTILP GETFFHKT
Sbjct: 3   MKIPMAFFTVASILISMADSRACGFPAIYNFGDSNSDTGGISAAFYPTILPCGETFFHKT 62

Query: 61  AGRGCDGRLILDFIAKHLELPYLSAYLNSIGANFRHGANFATGGSTIRRQNESVFENGVS 120
           AGRGCDGRLI+DF+AKHLELPYLSAYLNSIGANFRHGANFATGGSTIR QNESVFENGVS
Sbjct: 63  AGRGCDGRLIIDFLAKHLELPYLSAYLNSIGANFRHGANFATGGSTIRNQNESVFENGVS 122

Query: 121 PFSLDIQIVQFRQFKNRTIDRYLEATKEDSIRSTLPIPEDFSKALFTIDIGQNDLSVGFR 180
           PFSLDIQ+VQFRQFKNRTIDRY E T  D IRSTLP+PE+FS ALFTIDIGQNDLS GFR
Sbjct: 123 PFSLDIQVVQFRQFKNRTIDRYHEET-NDFIRSTLPVPEEFSNALFTIDIGQNDLSAGFR 182

Query: 181 LNFTDHQFRVAIPNIIAEFSAAIEELYKEGARAFWVHNTGPIGCIPVAIRSVSSPVTGDF 240
              T++QF++AIPNII EF AAIEELY+EGARAFWVHNTGPIGCIPVAIR+    V GD 
Sbjct: 183 -KMTNYQFQMAIPNIIGEFDAAIEELYREGARAFWVHNTGPIGCIPVAIRTT---VHGDL 242

Query: 241 DQNGCVRDQNDAALEFNRQLKEQVIKLRPKLSNASLVYVDVFAAKIKLIANAKEQGFMEK 300
           DQNGCV+ QNDAALEFNRQLKE+VI+LR KLS+ASLVYVDVFAAK++LIANAKE+GFME 
Sbjct: 243 DQNGCVKYQNDAALEFNRQLKERVIQLRAKLSDASLVYVDVFAAKLQLIANAKEEGFMEM 302

Query: 301 GAICCGYHEGNNHVWCGNKKIINGSEVYAGSCDDPSKLISWDGVHYTEAANRWIADQILN 360
           GAICCGYHEGN+HVWCGN+KIINGS+VYAGSCDDPS+ ISWDGVHYTEAANRWIA+QI+ 
Sbjct: 303 GAICCGYHEGNDHVWCGNQKIINGSQVYAGSCDDPSRFISWDGVHYTEAANRWIANQIIK 362

Query: 361 GSFSDPQVPITHAC 374
           GSFSDPQVPITHAC
Sbjct: 363 GSFSDPQVPITHAC 371

BLAST of Sed0001812 vs. ExPASy TrEMBL
Match: A0A1S3CN47 (GDSL esterase/lipase At5g14450-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103502686 PE=3 SV=1)

HSP 1 Score: 647.1 bits (1668), Expect = 4.4e-182
Identity = 312/375 (83.20%), Postives = 343/375 (91.47%), Query Frame = 0

Query: 1   MKIQMSLMVSS-ILISMADSKACNFPAIYNFGDSNSDTGGISAAFYPTILPTGETFFHKT 60
           MKIQ++  V+S ILISM DS+ACNFPAIYNFGDSNSDTGGISAAFYPTILP G+TFFHKT
Sbjct: 5   MKIQLAFTVASIILISMVDSRACNFPAIYNFGDSNSDTGGISAAFYPTILPCGQTFFHKT 64

Query: 61  AGRGCDGRLILDFIAKHLELPYLSAYLNSIGANFRHGANFATGGSTIRRQNESVFENGVS 120
           AGRGCDGRLI+DF AKHLELPYLSAYLNSIG NFRHGANFATGGSTIRRQNESVFENGVS
Sbjct: 65  AGRGCDGRLIIDFFAKHLELPYLSAYLNSIGTNFRHGANFATGGSTIRRQNESVFENGVS 124

Query: 121 PFSLDIQIVQFRQFKNRTIDRYLEATKEDSIRSTLPIPEDFSKALFTIDIGQNDLSVGFR 180
           PF LDIQ+VQFRQFKNRTIDRY+E   + SIRS+LP+PE+FSKALFTIDIGQNDLS GFR
Sbjct: 125 PFPLDIQVVQFRQFKNRTIDRYVEV--DGSIRSSLPVPEEFSKALFTIDIGQNDLSAGFR 184

Query: 181 LNFTDHQFRVAIPNIIAEFSAAIEELYKEGARAFWVHNTGPIGCIPVAIRSVSSPVTGDF 240
              T+ QF+ AIP+II EF+ A+E+LYKEG RAFWVHNTGPIGCIPVAIRS+S P  GD 
Sbjct: 185 -KMTNDQFQKAIPDIINEFATAVEDLYKEGGRAFWVHNTGPIGCIPVAIRSISKPNPGDL 244

Query: 241 DQNGCVRDQNDAALEFNRQLKEQVIKLRPKLSNASLVYVDVFAAKIKLIANAKEQGFMEK 300
           DQNGCV+ QN+AALEFNRQLKE+V+KLR KLSNA +VYVDV+AAKIKLIANAKE+GFMEK
Sbjct: 245 DQNGCVKYQNNAALEFNRQLKERVVKLRAKLSNAFIVYVDVYAAKIKLIANAKEEGFMEK 304

Query: 301 GAICCGYHEGNNHVWCGNKKIINGSEVYAGSCDDPSKLISWDGVHYTEAANRWIADQILN 360
           GAICCGYHEG +HVWCGNKKIINGSEVYAGSC DPSK ISWDGVHYTEAAN+WIA+QI++
Sbjct: 305 GAICCGYHEGVDHVWCGNKKIINGSEVYAGSCKDPSKFISWDGVHYTEAANQWIANQIVD 364

Query: 361 GSFSDPQVPITHACP 375
           GSFSDPQVPI HACP
Sbjct: 365 GSFSDPQVPIIHACP 376

BLAST of Sed0001812 vs. ExPASy TrEMBL
Match: A0A1S3CP81 (GDSL esterase/lipase At5g14450-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC103502686 PE=3 SV=1)

HSP 1 Score: 646.7 bits (1667), Expect = 5.8e-182
Identity = 312/375 (83.20%), Postives = 343/375 (91.47%), Query Frame = 0

Query: 1   MKIQMSLMVSS-ILISMADSKACNFPAIYNFGDSNSDTGGISAAFYPTILPTGETFFHKT 60
           MKIQ++  V+S ILISM DS+ACNFPAIYNFGDSNSDTGGISAAFYPTILP G+TFFHKT
Sbjct: 5   MKIQLAFTVASIILISMVDSRACNFPAIYNFGDSNSDTGGISAAFYPTILPCGQTFFHKT 64

Query: 61  AGRGCDGRLILDFIAKHLELPYLSAYLNSIGANFRHGANFATGGSTIRRQNESVFENGVS 120
           AGRGCDGRLI+DF AKHLELPYLSAYLNSIG NFRHGANFATGGSTIRRQNESVFENGVS
Sbjct: 65  AGRGCDGRLIIDFFAKHLELPYLSAYLNSIGTNFRHGANFATGGSTIRRQNESVFENGVS 124

Query: 121 PFSLDIQIVQFRQFKNRTIDRYLEATKEDSIRSTLPIPEDFSKALFTIDIGQNDLSVGFR 180
           PF LDIQ+VQFRQFKNRTIDRY+E   + SIRS+LP+PE+FSKALFTIDIGQNDLS GFR
Sbjct: 125 PFPLDIQVVQFRQFKNRTIDRYVE---DGSIRSSLPVPEEFSKALFTIDIGQNDLSAGFR 184

Query: 181 LNFTDHQFRVAIPNIIAEFSAAIEELYKEGARAFWVHNTGPIGCIPVAIRSVSSPVTGDF 240
              T+ QF+ AIP+II EF+ A+E+LYKEG RAFWVHNTGPIGCIPVAIRS+S P  GD 
Sbjct: 185 -KMTNDQFQKAIPDIINEFATAVEDLYKEGGRAFWVHNTGPIGCIPVAIRSISKPNPGDL 244

Query: 241 DQNGCVRDQNDAALEFNRQLKEQVIKLRPKLSNASLVYVDVFAAKIKLIANAKEQGFMEK 300
           DQNGCV+ QN+AALEFNRQLKE+V+KLR KLSNA +VYVDV+AAKIKLIANAKE+GFMEK
Sbjct: 245 DQNGCVKYQNNAALEFNRQLKERVVKLRAKLSNAFIVYVDVYAAKIKLIANAKEEGFMEK 304

Query: 301 GAICCGYHEGNNHVWCGNKKIINGSEVYAGSCDDPSKLISWDGVHYTEAANRWIADQILN 360
           GAICCGYHEG +HVWCGNKKIINGSEVYAGSC DPSK ISWDGVHYTEAAN+WIA+QI++
Sbjct: 305 GAICCGYHEGVDHVWCGNKKIINGSEVYAGSCKDPSKFISWDGVHYTEAANQWIANQIVD 364

Query: 361 GSFSDPQVPITHACP 375
           GSFSDPQVPI HACP
Sbjct: 365 GSFSDPQVPIIHACP 375

BLAST of Sed0001812 vs. ExPASy TrEMBL
Match: A0A5A7UPV8 (GDSL esterase/lipase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold257G00290 PE=3 SV=1)

HSP 1 Score: 636.0 bits (1639), Expect = 1.0e-178
Identity = 302/359 (84.12%), Postives = 330/359 (91.92%), Query Frame = 0

Query: 16  MADSKACNFPAIYNFGDSNSDTGGISAAFYPTILPTGETFFHKTAGRGCDGRLILDFIAK 75
           M DS+ACNFPAIYNFGDSNSDTGGISAAFYPTILP G+TFFHKTAGRGCDGRLI+DF AK
Sbjct: 1   MVDSRACNFPAIYNFGDSNSDTGGISAAFYPTILPCGQTFFHKTAGRGCDGRLIIDFFAK 60

Query: 76  HLELPYLSAYLNSIGANFRHGANFATGGSTIRRQNESVFENGVSPFSLDIQIVQFRQFKN 135
           HLELPYLSAYLNSIG NFRHGANFATGGSTIRRQNESVFENGVSPF LDIQ+VQFRQFKN
Sbjct: 61  HLELPYLSAYLNSIGTNFRHGANFATGGSTIRRQNESVFENGVSPFPLDIQVVQFRQFKN 120

Query: 136 RTIDRYLEATKEDSIRSTLPIPEDFSKALFTIDIGQNDLSVGFRLNFTDHQFRVAIPNII 195
           RTIDRY+E   + SIRS+LP+PE+FSKALFTIDIGQNDLS GFR   T+ QF+ AIP+II
Sbjct: 121 RTIDRYVE---DGSIRSSLPVPEEFSKALFTIDIGQNDLSAGFR-KMTNDQFQKAIPDII 180

Query: 196 AEFSAAIEELYKEGARAFWVHNTGPIGCIPVAIRSVSSPVTGDFDQNGCVRDQNDAALEF 255
            EF+ A+E+LYKEG RAFWVHNTGPIGCIPVAIRS+S P  GD DQNGCV+ QN+AALEF
Sbjct: 181 NEFATAVEDLYKEGGRAFWVHNTGPIGCIPVAIRSISKPNPGDLDQNGCVKYQNNAALEF 240

Query: 256 NRQLKEQVIKLRPKLSNASLVYVDVFAAKIKLIANAKEQGFMEKGAICCGYHEGNNHVWC 315
           NRQLKE+V+KLR KLSNA +VYVDV+AAKIKLIANAKE+GFMEKGAICCGYHEG +HVWC
Sbjct: 241 NRQLKERVVKLRAKLSNAFIVYVDVYAAKIKLIANAKEEGFMEKGAICCGYHEGVDHVWC 300

Query: 316 GNKKIINGSEVYAGSCDDPSKLISWDGVHYTEAANRWIADQILNGSFSDPQVPITHACP 375
           GNKKIINGSEVYAGSC DPSK ISWDGVHYTEAAN+WIA+QI++GSFSDPQVPI HACP
Sbjct: 301 GNKKIINGSEVYAGSCKDPSKFISWDGVHYTEAANQWIANQIVDGSFSDPQVPIIHACP 355

BLAST of Sed0001812 vs. TAIR 10
Match: AT5G14450.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 443.7 bits (1140), Expect = 1.4e-124
Identity = 207/367 (56.40%), Postives = 270/367 (73.57%), Query Frame = 0

Query: 7   LMVSSILISMADSKACNFPAIYNFGDSNSDTGGISAAFYPTILPTGETFFHKTAGRGCDG 66
           L + ++  S++    C FPAIYNFGDSNSDTGGISAAF P   P G+ FFH+  GR  DG
Sbjct: 22  LCLFAVTTSVSVQPTCTFPAIYNFGDSNSDTGGISAAFEPIRDPYGQGFFHRPTGRDSDG 81

Query: 67  RLILDFIAKHLELPYLSAYLNSIGANFRHGANFATGGSTIRRQNESVFENGVSPFSLDIQ 126
           RL +DFIA+ L LPYLSAYLNS+G+NFRHGANFATGGSTIRRQNE++F+ G+SPFSLD+Q
Sbjct: 82  RLTIDFIAERLGLPYLSAYLNSLGSNFRHGANFATGGSTIRRQNETIFQYGISPFSLDMQ 141

Query: 127 IVQFRQFKNRTIDRYLEATKEDSIRSTLPIPEDFSKALFTIDIGQNDLSVGFRLNFTDHQ 186
           I QF QFK R+   + +  K    R  LP  E+F+KAL+T DIGQNDLSVGFR    D Q
Sbjct: 142 IAQFDQFKARSALLFTQ-IKSRYDREKLPRQEEFAKALYTFDIGQNDLSVGFRTMSVD-Q 201

Query: 187 FRVAIPNIIAEFSAAIEELYKEGARAFWVHNTGPIGCIPVAIRSVSSPVTGDFDQNGCVR 246
            +  IP+I+   ++A+  +Y++G R FWVHNTGP GC+PV +  + +P  G  D++GCV+
Sbjct: 202 LKATIPDIVNHLASAVRNIYQQGGRTFWVHNTGPFGCLPVNMFYMGTPAPGYLDKSGCVK 261

Query: 247 DQNDAALEFNRQLKEQVIKLRPKLSNASLVYVDVFAAKIKLIANAKEQGFMEKGAICCGY 306
            QN+ A+EFNR+LKE VI LR +L+ A++ YVDV+ AK ++++N K+ GF     +CCGY
Sbjct: 262 AQNEMAMEFNRKLKETVINLRKELTQAAITYVDVYTAKYEMMSNPKKLGFANPLKVCCGY 321

Query: 307 HEGNNHVWCGNKKIINGSEVYAGSCDDPSKLISWDGVHYTEAANRWIADQILNGSFSDPQ 366
           HE  +H+WCGNK  +N +E+Y GSC +P   +SWDGVHYTEAAN+ +AD+ LNG  +DP 
Sbjct: 322 HEKYDHIWCGNKGKVNNTEIYGGSCPNPVMAVSWDGVHYTEAANKHVADRTLNGLLTDPP 381

Query: 367 VPITHAC 374
           VPIT AC
Sbjct: 382 VPITRAC 386

BLAST of Sed0001812 vs. TAIR 10
Match: AT3G26430.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 361.3 bits (926), Expect = 9.3e-100
Identity = 177/375 (47.20%), Postives = 246/375 (65.60%), Query Frame = 0

Query: 3   IQMSLMVSSILISMADSKACNFPAIYNFGDSNSDTGGISAAFYPTILPTGETFFHKTAGR 62
           ++  L+ S ++   A S +CNFPAI+NFGDSNSDTGG+SA+F     P G+TFFH  +GR
Sbjct: 8   VKCVLLASCLIHPRACSPSCNFPAIFNFGDSNSDTGGLSASFGQAPYPNGQTFFHSPSGR 67

Query: 63  GCDGRLILDFIAKHLELPYLSAYLNSIGANFRHGANFATGGSTIRRQNESVFENGVSPFS 122
             DGRLI+DFIA+ L LPYL+A+L+SIG+NF HGANFAT GST+R  N ++ ++GVSP S
Sbjct: 68  FSDGRLIIDFIAEELGLPYLNAFLDSIGSNFSHGANFATAGSTVRPPNATIAQSGVSPIS 127

Query: 123 LDIQIVQFRQFKNRTIDRYLEATKEDSIRSTLPIPEDFSKALFTIDIGQNDLSVGFRLNF 182
           LD+Q+VQF  F  R+    L   +    +  LP  E FS+AL+T DIGQNDL+ G +LN 
Sbjct: 128 LDVQLVQFSDFITRS---QLIRNRGGVFKKLLPKKEYFSQALYTFDIGQNDLTAGLKLNM 187

Query: 183 TDHQFRVAIPNIIAEFSAAIEELYKEGARAFWVHNTGPIGCIPVAIRSVSSPVTGDFDQN 242
           T  Q +  IP++  + S  I ++Y +G R FW+HNT P+GC+P  +     P +   D +
Sbjct: 188 TSDQIKAYIPDVHDQLSNVIRKVYSKGGRRFWIHNTAPLGCLPYVLDRFPVPAS-QIDNH 247

Query: 243 GCVRDQNDAALEFNRQLKEQVIKLRPKLSNASLVYVDVFAAKIKLIANAKEQGFMEKGAI 302
           GC   +N+ A  +N +LK +VI+LR +LS A+  YVD+++ K+ LI  AK+ GF      
Sbjct: 248 GCAIPRNEIARYYNSELKRRVIELRKELSEAAFTYVDIYSIKLTLITQAKKLGFRYPLVA 307

Query: 303 CCGY---HEGNNHVWCGNKKIINGSE-VYAGSCDDPSKLISWDGVHYTEAANRWIADQIL 362
           CCG+   +  N  + CG K +I G E V A SC+D S  +SWDG+H+TE  N WI  QI 
Sbjct: 308 CCGHGGKYNFNKLIKCGAKVMIKGKEIVLAKSCNDVSFRVSWDGIHFTETTNSWIFQQIN 367

Query: 363 NGSFSDPQVPITHAC 374
           +G+FSDP +P+  AC
Sbjct: 368 DGAFSDPPLPVKSAC 378

BLAST of Sed0001812 vs. TAIR 10
Match: AT1G67830.1 (alpha-fucosidase 1 )

HSP 1 Score: 350.1 bits (897), Expect = 2.2e-96
Identity = 179/365 (49.04%), Postives = 239/365 (65.48%), Query Frame = 0

Query: 3   IQMSLMVSSILISMADS---KACNFPAIYNFGDSNSDTGGISAAFYPTILPTGETFFHKT 62
           I  SL   S+L S++ S     C+FPAI+NFGDSNSDTGG+SAAF     P G +FF   
Sbjct: 4   ILSSLFALSLLSSLSPSTHAHQCHFPAIFNFGDSNSDTGGLSAAFGQAGPPHGSSFFGSP 63

Query: 63  AGRGCDGRLILDFIAKHLELPYLSAYLNSIGANFRHGANFATGGSTIRRQNESVFENGVS 122
           AGR CDGRL++DFIA+ L LPYLSA+L+S+G+NF HGANFAT GS IR  N ++ ++G S
Sbjct: 64  AGRYCDGRLVIDFIAESLGLPYLSAFLDSVGSNFSHGANFATAGSPIRALNSTLRQSGFS 123

Query: 123 PFSLDIQIVQFRQFKNRTIDRYLEATKEDSIRSTLPIPEDFSKALFTIDIGQNDLSVGFR 182
           PFSLD+Q VQF  F NR+       ++    ++ LP  + FSKAL+T DIGQNDL+ G+ 
Sbjct: 124 PFSLDVQFVQFYNFHNRS---QTVRSRGGVYKTMLPESDSFSKALYTFDIGQNDLTAGYF 183

Query: 183 LNFTDHQFRVAIPNIIAEFSAAIEELYKEGARAFWVHNTGPIGCIPVAIRSVSSPVTGDF 242
            N T  Q    +P II++F  AI+ +Y +G R FW+HNTGPIGC+   I    +  + DF
Sbjct: 184 ANKTVEQVETEVPEIISQFMNAIKNIYGQGGRYFWIHNTGPIGCLAYVIERFPNKAS-DF 243

Query: 243 DQNGCVRDQNDAALEFNRQLKEQVIKLRPKLSNASLVYVDVFAAKIKLIANAKEQGFMEK 302
           D +GCV   N  A +FN  LK+ VI+LR  LS A++ YVDV++ K +L  +A+  GF   
Sbjct: 244 DSHGCVSPLNHLAQQFNHALKQAVIELRSSLSEAAITYVDVYSLKHELFVHAQGHGFKGS 303

Query: 303 GAICCGY---HEGNNHVWCGNKKIINGSEVYAGS-CDDPSKLISWDGVHYTEAANRWIAD 361
              CCG+   +  N  + CG KKI+ G EVY G  CD+P K + WDGVH+T+AAN++I D
Sbjct: 304 LVSCCGHGGKYNYNKGIGCGMKKIVKGKEVYIGKPCDEPDKAVVWDGVHFTQAANKFIFD 363

BLAST of Sed0001812 vs. TAIR 10
Match: AT3G27950.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 335.5 bits (859), Expect = 5.5e-92
Identity = 170/355 (47.89%), Postives = 229/355 (64.51%), Query Frame = 0

Query: 19  SKACNFPAIYNFGDSNSDTGGISAAFYPTILPTGETFFHKTAGRGCDGRLILDFIAKHLE 78
           S +CNFPA++NFGDSNSDTG ISAA      P G  FF ++AGR  DGRLI+DFI ++L 
Sbjct: 25  SSSCNFPAVFNFGDSNSDTGAISAAIGEVPPPNGVAFFGRSAGRHSDGRLIIDFITENLT 84

Query: 79  LPYLSAYLNSIGANFRHGANFATGGSTIRRQNESVFENGVSPFSLDIQIVQFRQFKNRTI 138
           LPYL+ YL+S+GAN+RHGANFATGGS IR           SPF L  Q+ QF  FK RT+
Sbjct: 85  LPYLTPYLDSVGANYRHGANFATGGSCIRPTLAC-----FSPFHLGTQVSQFIHFKTRTL 144

Query: 139 DRYLEATKEDSIRSTLPIPEDFSKALFTIDIGQNDLSVGFRLNFTDHQFRVAIPNIIAEF 198
             Y +               DFSKAL+T+DIGQNDL++GF+ N T+ Q +  IP II  F
Sbjct: 145 SLYNQT-------------NDFSKALYTLDIGQNDLAIGFQ-NMTEEQLKATIPLIIENF 204

Query: 199 SAAIEELYKEGARAFWVHNTGPIGCIPVAIRSVSSPVTGDFDQNGCVRDQNDAALEFNRQ 258
           + A++ LYKEGAR F +HNTGP GC+P  +++  +      D  GC++  N+ A+EFN+Q
Sbjct: 205 TIALKLLYKEGARFFSIHNTGPTGCLPYLLKAFPAIPR---DPYGCLKPLNNVAIEFNKQ 264

Query: 259 LKEQVIKLRPKLSNASLVYVDVFAAKIKLIANAKEQGFMEKGAICCGYHEGNNHVWCGNK 318
           LK ++ +L+ +L ++   YVDV++AK  LI  AK  GF++    CC    G   + CG  
Sbjct: 265 LKNKITQLKKELPSSFFTYVDVYSAKYNLITKAKALGFIDPFDYCCVGAIGRG-MGCGKT 324

Query: 319 KIINGSEVYAGSCDDPSKLISWDGVHYTEAANRWIADQILNGSFSDPQVPITHAC 374
             +NG+E+Y+ SC +    ISWDG+HYTE AN  +A++IL+GS SDP +P   AC
Sbjct: 325 IFLNGTELYSSSCQNRKNFISWDGIHYTETANMLVANRILDGSISDPPLPTQKAC 356

BLAST of Sed0001812 vs. TAIR 10
Match: AT1G54790.3 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 283.1 bits (723), Expect = 3.2e-76
Identity = 153/361 (42.38%), Postives = 217/361 (60.11%), Query Frame = 0

Query: 11  SILISMADSKACNFPAIYNFGDSNSDTGG-ISAAFYPTILPTGETFFHKTAGRGCDGRLI 70
           S+ +  + S    +PAI NFGDSNSDTG  ISA       P G+T+F+  +GR CDGRLI
Sbjct: 17  SLFLPSSFSIILKYPAIINFGDSNSDTGNLISAGIENVNPPYGQTYFNLPSGRYCDGRLI 76

Query: 71  LDFIAKHLELPYLSAYLNSIG-ANFRHGANFATGGSTIRRQNESVFENGVSPFSLDIQIV 130
           +DF+   ++LP+L+ YL+S+G  NF+ G NFA  GSTI   N +     VSPFS D+QI 
Sbjct: 77  VDFLLDEMDLPFLNPYLDSLGLPNFKKGCNFAAAGSTILPANPT----SVSPFSFDLQIS 136

Query: 131 QFRQFKNRTIDRYLEATKEDSIRSTLPIPEDFSKALFTIDIGQNDLSVGFRLNFTDHQFR 190
           QF +FK+R I+   +  ++      LP  + +SK L+ IDIGQND++  F     D Q  
Sbjct: 137 QFIRFKSRAIELLSKTGRK--YEKYLPPIDYYSKGLYMIDIGQNDIAGAFYSKTLD-QVL 196

Query: 191 VAIPNIIAEFSAAIEELYKEGARAFWVHNTGPIGCIPVAIRSVSSPVTGDFDQNGCVRDQ 250
            +IP+I+  F A ++ LY+EG R  W+HNTGP+GC+   I    +  T   D+ GCV   
Sbjct: 197 ASIPSILETFEAGLKRLYEEGGRNIWIHNTGPLGCLAQNIAKFGTDST-KLDEFGCVSSH 256

Query: 251 NDAALEFNRQLKEQVIKLRPKLSNASLVYVDVFAAKIKLIANAKEQGFMEKGAICCGYHE 310
           N AA  FN QL     K + +  +A++ YVD+F+ K  LIAN    GF +    CCG   
Sbjct: 257 NQAAKLFNLQLHAMSNKFQAQYPDANVTYVDIFSIKSNLIANYSRFGFEKPLMACCGVGG 316

Query: 311 G----NNHVWCGNKKIINGSEVYAGSCDDPSKLISWDGVHYTEAANRWIADQILNGSFSD 366
                ++ + CG  K+++G  V A +C+D S+ I+WDG+HYTEAAN +++ QIL G +SD
Sbjct: 317 APLNYDSRITCGQTKVLDGISVTAKACNDSSEYINWDGIHYTEAANEFVSSQILTGKYSD 369