Sed0000088 (gene) Chayote v1

Overview
NameSed0000088
Typegene
OrganismSechium edule (Chayote v1)
DescriptionHistidine--tRNA ligase
LocationLG14: 3959361 .. 3966097 (-)
RNA-Seq ExpressionSed0000088
SyntenySed0000088
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCTTTCGATCTTTCGACCAACTTAAAAAAAAAATTTATACACGACACTAGAGTTAAAATAGATTGACACCAAACAAGTGGGCTACCCGGTTTTGTTTGCGGTACTAAGCGCACACAGTCGAAACCCACCAGCGAGCAATTTTGTGAGCTGAGTCTGTACAGAGTCGACCACCATGGCTGCGAAGCCTTCATCGCTCCTTATCTTCAAGCCTCGCCTCAATCTCCATCTCCATCTCAAACCCTTCATTTTCTCATCTTCATCACTCGCTTTCAACATCATTCTCAGATTCCCCTTTCCCAAGCCCTCGTTCTTCCCCAATCGTAAATTCCATTCCTCACGCGCCGCCTCTTCCGCACAGTCTCTCAGTGGCGGCGGCGGAAGGTCGGGGGCGCTAGCTCCGGCGCCTGCCACTGAAGAGGTGCAGAAGATCGATGTCAATCCTCCCAAGGGAACCAGAGATTTTCCACCGGAGGATATGCGACTCCGGAATTGGCTGTTCAGTAATTTCAGAGAGGTTTTGATTTCCTTTGGTTTTGATTTTTGTTAGGTTTTTGTGACTCTGAGGTTTTGATTCTATTGTTTATTAGGTTTCACGAGTGTACGGGTTTGAGGAGGTTGATTTTCCGGTGCTCGAGTCGGAGGCGCTGTTTATTAGGAAAGCAGGTGAAGAAATACGAGACCAGGTTGGTGTTTATGTAATTGTTTGCTTTGAGTTCCGTTCATTTGCTAATTTTGGACACTAATTTCTTGAATCCTGTAAAAACTTATAGGATGAGTAATCGTATTTTATTTCTTGCTCAAATTCGAATGTTGTTAATCTGGATTATCGTCTGATTAGTTAAATGCTTGTCTTTATTATTGGGATGCCAACTAAGTATTAGATTAGTGGAATATAATGAGTATATAAGTGACGACAACCATCGATATTGGTATGGCACTATGGCTTAAGCCTTTTGGGTGAACTAAAAAATAAATTCATGAGGGTGCATGCCGAAAGTGGACTATAATGTGGAGATTCGGGTAGGGTTTGTCATCCTTATCGGTGTCAGAGGAGGCATTGTTTCATCCGTTCTGTAAGGAGGGAAGGATTCTTTAAGTTGAACTTTGCTGCTCTTCTTTATAGAATGTTTAACATGAGAGAATATGAGAATTGTAGAAGGCTTGTAAGTATGCCAGTAGCAAATGTAGAAAGAACCAAATGAAATCCCAATATCTAATTCTTACCCTATTTGATTCATCCAACCAAAGCTGAAAAATAAAACTCTTTCCAATCTCTGTTAACAATTTTTTAAGCGATTAAGTGCAGCAATTTTTGTTGAGTATTAAAGCCGTTTTGCCCTATTTCGTGCAAGTTTGAGTGAGTAATTAACCATATGAGATATGGTAACTCAACCATTGTGAGTTGGCCTTGAGAATTATGAGGTTATGATCTCTTATGATGGCTATCTATTTAAGAATTAATTTCTCATGTTTTTCCTTGACAACCAAATATTGTAGGGTTAGACAATTTGTGTTTGTCCCATGGGAATAGTTAAGGTGGGCGTAAGTTGTCTTAGACAGTCATGAATATATAAAAAAAGATTTGGTAACTCAAATGATATTAGCAATGAAATTCTTTGTGTGATCATTTTAACACTGTTAACATGTTATCTTACCAACCCCATCTATCTCACACTTTCGGTGTTAAACTTATTTTTGTTTCAAATTGATTGGGGCTTGTATACTATGCACATCCTTGCTTCTTCCTACCACTTTCCCTGCCCCATCCCATCCATACATACAGCTTCTTGAAATTGGGACACGTTTATAGAAGAACATTGCAACAAAATCTAGTTGATCTTGGAATGTTAAGGTGTATAACAGTTGATTAATTTTTTTTATGTAGCTCTATTGTTTTGAAGACCGAGGAAATCGTCGAGTTGCTTTGAGGCCAGAGCTTACCCCTTCTTTGGCAAGATTAGTGATCCAGAAGGGGTATTTTTCCTTTTATGCAATTTTCTTCCATATCTTGCAATGTGCTGCAATATTTTTCACTTTTTTTAATAATAAAAAAAAGGAATTTGGATGTGAAAATTTGTCTATTTATTTTTTTGAATTATCTTTGTATCCAGGTACAGATGTCAGAATTTGATCTCCATCTTTTTTTCTGTTCAGAAAATCTTTGTCCCTTCCATTGAAATGGTTTGCTATAGGGCAATGTTGGCGGTATGAGAGAATGACAAGAGGTAGGCGTCGTGAGCATTACCAATGGAATATGGACATCATTGGTGTACCCGAGGTTACGGTATGTGAAGTTACAAACTTGAAGAAATCATAATCATGCAACTTTATGTGACTATCCCTTCATACATGGGCTGTCCCTTCATGTCATGCCTTTAGGGTCCGTATAGTGAGCTGTGTGTATTCTATTTCATCATCATGTTTTATGGGAAGCAAATAAAAAAAAATTGGTTAAACAAAGTGTGTTTGATGGTATCAAGTCCCCTATATCATTCGGTAAAGTTGCTGCAGGGTTTTCCTAAGTTTTGACCGCGTCAGTATTTTTTAGCATGTTTAGAAGAAAGGAGAGTATTTGCATGCTTTGAAGATGTGAAGTTCTGCTGGTTTTTGATGTTTTGCTTCCAGCCTCGGGCAATATAAATTATGATAAGTTTTCTATAGATGCAAGAAGATTTATTTTCTGGTGCAGCAAGGACGATGATGTAAAGTTGGATTTTTTAGACATTTTTTGTATGGATATTTTTGTTTGTTAATTTTTTAGTCTTCTAAAATGCTTGTGGGTTCAGAGTTTGTAGTTTAGAGTCGTCCTATCCTATGTTAGTTGCTACCTTTAATTTCTTCAATGCAAAATTTAGGTTTCCTAGAAAAAATGAATTGCCCTCGTTTGAGATTCCTATGAGTAAATCGATTTGGAGCCTTGACCGACACCCTACCTTTGTGTATCATCATCTCATTCATCTTGACATGGCCTAGTTGTAATATAGTTTACTCGTTGGTACCTGCCTTGAATTGAATGTCAAAACTTGTTTGAACGGACCATTTCCTATGTGTGCTCATTTTATTATTTTATTGTTTTATCCAGGCTGAAGCGGAGCTTCTATCTTCTATTGTTACCTTTTTCAAGCGAATTGGAATCACAGCATCAGATGTTGGATTTAAGATTTCCAGTCGAAAGGTATTTATTTTTCTGCCTCTTGTATGTTCATTCCAATAAATGTAGTACTTGCTTTGCTTTAGTAGTTATTAAACATTCATAGTTATAGACTTATAGTTGGTGTAAATGCCTTTTGACTGTGGTTTGGTTGTAGGTTTTGCAAGAAGTATTAACATGTTATGGAATACCTGAAAATCTGTTTGGCAAGGTTTGCATCATCATAGACAAGGTTAGTGATTTTCGATTTCAAGTTCCATAAAATTTTAATATTTTTGAGACCTTTCCCTATGCATATCCGAATGTACTTTTTTCTCCCTGTTTCCCCTGGCTATCAATCTTAAGAACAGATAGAGAAAATTCCATTGGATGAGATGAAAAAGGAACTAAAGGTCGTAGGCGTATCAGAAGAGGCTATCGAGGAGCTGTTGCAAGTTCTTTCAGTAAAGTCTTTGACAAAGCTTGAAGGTATTTTCATAATGCTTTAGTTTTTGACAAATTAATTGGAATATGTATGTGTGAATTCTGATGGAAGGCATTGAAATTGATGGAAGTGTAATTGTTATTCTTGCATAGAGATACTTGGAGGTGGTGGCGAAGCGTTGTCTGATCTGAAGCAACTATTTTCTCTTGCTGAAAAGTTTGGGTATGCTGATTGGATCCAATTTGATGCGTCCATTGTCCGTGGCCTTGCTTATTACACCGGCATTGTTTTTGAGGTTAGTTTCATCAGTAATATTTGGTACCACATCTAAAACTGCATGCTGATTTTTTTTTTTATTGATATTCGTGACTGTCCGTGCCCGCTTTCACGCACCTTGAATAATGTAACGGGACTTGCTGCTTAACCTTACAATATTTGGATGTCAAAGAAACCCATAGGAAATTAATTTTTAGGTAGGTGGCTACCATGGATTGAACCCATCACCTTTAGCATCCCAAGACTCTCTTTGACTATTAGGCCACTCTATGATGTTAAAGTGCTTGATGGGTTGATTTTCATTCCCGCATATAAGTGAAATAAATGTAGGCAAAGCAGCCCATTAAAAGACTACAGTCCTTGGTATAATATACTATCAATTTAGGCTTATAAAGATTGCTTCAACCTTTGAGTTTCTTTATTTTGTTTATTTTACAAATATATTTAAATCCCTACCAAATTTTGAAATCTAAAAAAGTAGGCCCTGTTTGATAACCATTTGTTTTTTCTATCTGAAAAAAAAAACTGTTTGTTTTTAAATTTTTAAAAAATTAAGTTTATAAACACTACTTCCACATACGAACTTTGAGTTTTTAACCATTATGTGGTGTTTTAGTGGTTAAAGCATAACAAATACCTACTTTAAAAAAAAAAAAAGCATAACAAATACCATTTTCAAAAATCAAATGATTATTAAACCAGGCTTAAAATTTTCAGATTATCTATCGAAATGTGTTCTTATTCCTTTTCATTGTCTTGTTTATGAGTATTTTCTTTTGAACTCTATCTTCATTTAAAATAAAAGCTAATAGCCATATTCATTTCTTGCTCATTGTTTTATGGAAATAGTTGGTTACTTGGTCAAAGTTGTATTTTCCTCTCTTTTTTGTATCGGTAAAATTAAGTATGATAATTGTTCCTTCTGGCCATGTCTTATTTTCATTTTAGTAATGAAGATGCATTTCAGGGTTTTGACAGAGGGGGAAAGCTGCGAGCCATATGCGGTGGAGGTCGATATGACCGGTTGCTTTCTACATTTGGTGGCGACGATATTCCAGCTTGTGGCTTTGGTTTTGGCGATGCCGTAATAGTAGAGGTAAATTTTAAGCCGATCTCTGCTCCTTTATGCCATGGCAAACCTCTATATAAGAATGGCATGTTTGAGAGTAATCAAAATAATTTTATCATACCCAAAATAACTAGGGCATGACAAAAGTAATTTTGACCATTAAAAAAATCACTGTTAAAGATGCCGTTATTTGATATCAACTGTCAAGGGGAGAAGGGACACATCTAACTGCAACTCCCTTTAATCATTAGTATCATGCTTCCTGATCAACTGCGGTCAACTGAATGTTAAACAACCACCTTGAATTGACACATAGTAAAGAGTCTTTTAGTTCAAAGTTCAAACCATGTAGGCCATTTTTTTAACTGAACGTTTAGGGGAGGGAGATTCAAACCTGTAGCCTTACAAGATTATACAAGATGTTGCTAGTGCTCTGGGCGGAGGCCATTTAACATAATATCCTACTGATTTCTTTATATGAAATGATTTAAGATAAATGGTTAGATTCACTTGAGGAGTATAAATTATCCATACATATAATTCAAAAAGAAAAGAGGAAAAAACTAATTTTACCCTCATACCTAATGTGAAATCAATACGCCCTATAAAATAAATTAGAAAATCAAACAAAGCTCAACTAACAGCACTCAAATTCAAAGTCATAGACATATACAAAAGTACAAAACTAGGTAAAATAAAATAAAATAAAGCCAATAGAAAAATTGTTTTTTATTTTTTATTTTTTATATCGGGTAGTGCCTAGTCCAGTCACTTCGACTATTCTTACGAGACATATCACTTGTCCCTATTAAGAAAACCCGTAGAAAATGAATTCTTAGGTAGGTGGCTTCCATTTAATCTTTAGACCACTAGGCTGACGATTTATGATGGTAAAAAGCGTTTTTTGCAAAAACGGTCTCCTTCCACGGCAAGGTTGCTCATATCTAACTGCAAATTCAAGGGTGCACGTTGAAACCATGAAAGAAACCAGTTCATTAAGTCGGTTCTGTCTGTTAGTTGGTTGAAGTTCAGTTTGTCATTGACGAATCTGGAATGACTTTCTCAACGTTTGAAAACACCCTTTTAAGCATTGGAAAGTGATTTCAAACCGGCTCTAAAATGGATTTTGGGGTCATAATTTTTCATTAATGTATACTTGTTTTGGTCATACTTCTCAATGAATTCGATCTTAGATAATTGTTTGGCTCTCGAACGGGCTTTTGTTGTAGTTGCTCAAAGAAAAGAAGTTGCTGCCAGAACTGAATGTTGGAATAGAGAACATTGTTTGTGCTCTGGATCCTGATCTCCAAGGTGCAGCTTCCACAGTTGCTACCATTCTCAGGGAAAAAGGTCAAACTGTTGATTTGGTCTTGGAGAACAAACCTCTTAAATGGTATCCTTCACAATAACAAAACTTGATCTTCATCAAAATCATCACATTTTTTTATCCGTATTGGTTGTTTATCGTTTGCAGGGTGTTTAAACGAGCCACGCGGATAAACGCGCAACGGTTGATACTAGTTGGAAGTTCAGAATGGCAACGAGGCGTGGTCGGAGTTAAAGATCTTTCATCGGGTGAACAACGTGAGGTCAAACTTGATGAACTGAACTAACTTAACTTCATTCTTTCTTGTTCCGTAGATTATTTATTTATATGCTGTGCAAGATTAGTTTAGATTTCAAAATTTTCCATTCTGGTTCTGCATTTACGAAAATTTCAATTTCAGATATTTGTGTTGAAAATTTATTGCAAAATTGTGGTTACATGAGATACATTTCAATATTCAATTTGAATTTTTGTGTTAAATAGTTGGC

mRNA sequence

ATGCTTTCGATCTTTCGACCAACTTAAAAAAAAAATTTATACACGACACTAGAGTTAAAATAGATTGACACCAAACAAGTGGGCTACCCGGTTTTGTTTGCGGTACTAAGCGCACACAGTCGAAACCCACCAGCGAGCAATTTTGTGAGCTGAGTCTGTACAGAGTCGACCACCATGGCTGCGAAGCCTTCATCGCTCCTTATCTTCAAGCCTCGCCTCAATCTCCATCTCCATCTCAAACCCTTCATTTTCTCATCTTCATCACTCGCTTTCAACATCATTCTCAGATTCCCCTTTCCCAAGCCCTCGTTCTTCCCCAATCGTAAATTCCATTCCTCACGCGCCGCCTCTTCCGCACAGTCTCTCAGTGGCGGCGGCGGAAGGTCGGGGGCGCTAGCTCCGGCGCCTGCCACTGAAGAGGTGCAGAAGATCGATGTCAATCCTCCCAAGGGAACCAGAGATTTTCCACCGGAGGATATGCGACTCCGGAATTGGCTGTTCAGTAATTTCAGAGAGGTTTCACGAGTGTACGGGTTTGAGGAGGTTGATTTTCCGGTGCTCGAGTCGGAGGCGCTGTTTATTAGGAAAGCAGGTGAAGAAATACGAGACCAGCTCTATTGTTTTGAAGACCGAGGAAATCGTCGAGTTGCTTTGAGGCCAGAGCTTACCCCTTCTTTGGCAAGATTAGTGATCCAGAAGGGAAAATCTTTGTCCCTTCCATTGAAATGGTTTGCTATAGGGCAATGTTGGCGGTATGAGAGAATGACAAGAGGTAGGCGTCGTGAGCATTACCAATGGAATATGGACATCATTGGTGTACCCGAGGTTACGGCTGAAGCGGAGCTTCTATCTTCTATTGTTACCTTTTTCAAGCGAATTGGAATCACAGCATCAGATGTTGGATTTAAGATTTCCAGTCGAAAGGTTTTGCAAGAAGTATTAACATGTTATGGAATACCTGAAAATCTGTTTGGCAAGGTTTGCATCATCATAGACAAGATAGAGAAAATTCCATTGGATGAGATGAAAAAGGAACTAAAGGTCGTAGGCGTATCAGAAGAGGCTATCGAGGAGCTGTTGCAAGTTCTTTCAGTAAAGTCTTTGACAAAGCTTGAAGAGATACTTGGAGGTGGTGGCGAAGCGTTGTCTGATCTGAAGCAACTATTTTCTCTTGCTGAAAAGTTTGGGTATGCTGATTGGATCCAATTTGATGCGTCCATTGTCCGTGGCCTTGCTTATTACACCGGCATTGTTTTTGAGGGTTTTGACAGAGGGGGAAAGCTGCGAGCCATATGCGGTGGAGGTCGATATGACCGGTTGCTTTCTACATTTGGTGGCGACGATATTCCAGCTTGTGGCTTTGGTTTTGGCGATGCCGTAATAGTAGAGTTGCTCAAAGAAAAGAAGTTGCTGCCAGAACTGAATGTTGGAATAGAGAACATTGTTTGTGCTCTGGATCCTGATCTCCAAGGTGCAGCTTCCACAGTTGCTACCATTCTCAGGGAAAAAGGTCAAACTGTTGATTTGGTCTTGGAGAACAAACCTCTTAAATGGGTGTTTAAACGAGCCACGCGGATAAACGCGCAACGGTTGATACTAGTTGGAAGTTCAGAATGGCAACGAGGCGTGGTCGGAGTTAAAGATCTTTCATCGGGTGAACAACGTGAGGTCAAACTTGATGAACTGAACTAACTTAACTTCATTCTTTCTTGTTCCGTAGATTATTTATTTATATGCTGTGCAAGATTAGTTTAGATTTCAAAATTTTCCATTCTGGTTCTGCATTTACGAAAATTTCAATTTCAGATATTTGTGTTGAAAATTTATTGCAAAATTGTGGTTACATGAGATACATTTCAATATTCAATTTGAATTTTTGTGTTAAATAGTTGGC

Coding sequence (CDS)

ATGGCTGCGAAGCCTTCATCGCTCCTTATCTTCAAGCCTCGCCTCAATCTCCATCTCCATCTCAAACCCTTCATTTTCTCATCTTCATCACTCGCTTTCAACATCATTCTCAGATTCCCCTTTCCCAAGCCCTCGTTCTTCCCCAATCGTAAATTCCATTCCTCACGCGCCGCCTCTTCCGCACAGTCTCTCAGTGGCGGCGGCGGAAGGTCGGGGGCGCTAGCTCCGGCGCCTGCCACTGAAGAGGTGCAGAAGATCGATGTCAATCCTCCCAAGGGAACCAGAGATTTTCCACCGGAGGATATGCGACTCCGGAATTGGCTGTTCAGTAATTTCAGAGAGGTTTCACGAGTGTACGGGTTTGAGGAGGTTGATTTTCCGGTGCTCGAGTCGGAGGCGCTGTTTATTAGGAAAGCAGGTGAAGAAATACGAGACCAGCTCTATTGTTTTGAAGACCGAGGAAATCGTCGAGTTGCTTTGAGGCCAGAGCTTACCCCTTCTTTGGCAAGATTAGTGATCCAGAAGGGAAAATCTTTGTCCCTTCCATTGAAATGGTTTGCTATAGGGCAATGTTGGCGGTATGAGAGAATGACAAGAGGTAGGCGTCGTGAGCATTACCAATGGAATATGGACATCATTGGTGTACCCGAGGTTACGGCTGAAGCGGAGCTTCTATCTTCTATTGTTACCTTTTTCAAGCGAATTGGAATCACAGCATCAGATGTTGGATTTAAGATTTCCAGTCGAAAGGTTTTGCAAGAAGTATTAACATGTTATGGAATACCTGAAAATCTGTTTGGCAAGGTTTGCATCATCATAGACAAGATAGAGAAAATTCCATTGGATGAGATGAAAAAGGAACTAAAGGTCGTAGGCGTATCAGAAGAGGCTATCGAGGAGCTGTTGCAAGTTCTTTCAGTAAAGTCTTTGACAAAGCTTGAAGAGATACTTGGAGGTGGTGGCGAAGCGTTGTCTGATCTGAAGCAACTATTTTCTCTTGCTGAAAAGTTTGGGTATGCTGATTGGATCCAATTTGATGCGTCCATTGTCCGTGGCCTTGCTTATTACACCGGCATTGTTTTTGAGGGTTTTGACAGAGGGGGAAAGCTGCGAGCCATATGCGGTGGAGGTCGATATGACCGGTTGCTTTCTACATTTGGTGGCGACGATATTCCAGCTTGTGGCTTTGGTTTTGGCGATGCCGTAATAGTAGAGTTGCTCAAAGAAAAGAAGTTGCTGCCAGAACTGAATGTTGGAATAGAGAACATTGTTTGTGCTCTGGATCCTGATCTCCAAGGTGCAGCTTCCACAGTTGCTACCATTCTCAGGGAAAAAGGTCAAACTGTTGATTTGGTCTTGGAGAACAAACCTCTTAAATGGGTGTTTAAACGAGCCACGCGGATAAACGCGCAACGGTTGATACTAGTTGGAAGTTCAGAATGGCAACGAGGCGTGGTCGGAGTTAAAGATCTTTCATCGGGTGAACAACGTGAGGTCAAACTTGATGAACTGAACTAA

Protein sequence

MAAKPSSLLIFKPRLNLHLHLKPFIFSSSSLAFNIILRFPFPKPSFFPNRKFHSSRAASSAQSLSGGGGRSGALAPAPATEEVQKIDVNPPKGTRDFPPEDMRLRNWLFSNFREVSRVYGFEEVDFPVLESEALFIRKAGEEIRDQLYCFEDRGNRRVALRPELTPSLARLVIQKGKSLSLPLKWFAIGQCWRYERMTRGRRREHYQWNMDIIGVPEVTAEAELLSSIVTFFKRIGITASDVGFKISSRKVLQEVLTCYGIPENLFGKVCIIIDKIEKIPLDEMKKELKVVGVSEEAIEELLQVLSVKSLTKLEEILGGGGEALSDLKQLFSLAEKFGYADWIQFDASIVRGLAYYTGIVFEGFDRGGKLRAICGGGRYDRLLSTFGGDDIPACGFGFGDAVIVELLKEKKLLPELNVGIENIVCALDPDLQGAASTVATILREKGQTVDLVLENKPLKWVFKRATRINAQRLILVGSSEWQRGVVGVKDLSSGEQREVKLDELN
Homology
BLAST of Sed0000088 vs. NCBI nr
Match: XP_038883346.1 (histidine--tRNA ligase, chloroplastic/mitochondrial [Benincasa hispida])

HSP 1 Score: 924.1 bits (2387), Expect = 5.2e-265
Identity = 466/509 (91.55%), Postives = 491/509 (96.46%), Query Frame = 0

Query: 1   MAAKPSSLLIFKPRLNLHLHLKPFIFSSSSLAFNIILRFPFPKPSFFPNRKFHSS-RAAS 60
           MA KPSSLLIF+PRLN  L LKPFIFSSSSLAF+IIL+FPFPKPSFF NR FHSS  A S
Sbjct: 1   MATKPSSLLIFQPRLN--LRLKPFIFSSSSLAFSIILKFPFPKPSFFVNRNFHSSLTAVS 60

Query: 61  SAQSLSGG---GGRSGALAPAPATEEVQKIDVNPPKGTRDFPPEDMRLRNWLFSNFREVS 120
           SAQS++ G   GGRSGAL+PAP TEE+QKIDVNPPKGTRDFPPEDMRLRNWLF+NFREVS
Sbjct: 61  SAQSITDGGTSGGRSGALSPAPVTEELQKIDVNPPKGTRDFPPEDMRLRNWLFNNFREVS 120

Query: 121 RVYGFEEVDFPVLESEALFIRKAGEEIRDQLYCFEDRGNRRVALRPELTPSLARLVIQKG 180
           RVYGFEEVD+PVLESEALF+RKAGEEIRDQLYCFEDRGNRRVALRPELTPSLARLVIQKG
Sbjct: 121 RVYGFEEVDYPVLESEALFVRKAGEEIRDQLYCFEDRGNRRVALRPELTPSLARLVIQKG 180

Query: 181 KSLSLPLKWFAIGQCWRYERMTRGRRREHYQWNMDIIGVPEVTAEAELLSSIVTFFKRIG 240
           KSLSLP+KWFAIGQCWRYERMTRGRRREHYQWNMDIIGVPEVTAEAELLSSIVTFFKR+G
Sbjct: 181 KSLSLPIKWFAIGQCWRYERMTRGRRREHYQWNMDIIGVPEVTAEAELLSSIVTFFKRVG 240

Query: 241 ITASDVGFKISSRKVLQEVLTCYGIPENLFGKVCIIIDKIEKIPLDEMKKELKVVGVSEE 300
           ITASDVGFK+SSRKVLQ+VL CYGIPEN+FGKVCIIIDKIEKIPLDE+KKELKVVG+SE+
Sbjct: 241 ITASDVGFKVSSRKVLQDVLKCYGIPENMFGKVCIIIDKIEKIPLDEIKKELKVVGISED 300

Query: 301 AIEELLQVLSVKSLTKLEEILGGGGEALSDLKQLFSLAEKFGYADWIQFDASIVRGLAYY 360
           AIEELLQVLSVKSLTKLEE+LGGGGEALSDLKQLFSLAEKFGY+DWIQFDASIVRGLAYY
Sbjct: 301 AIEELLQVLSVKSLTKLEEVLGGGGEALSDLKQLFSLAEKFGYSDWIQFDASIVRGLAYY 360

Query: 361 TGIVFEGFDRGGKLRAICGGGRYDRLLSTFGGDDIPACGFGFGDAVIVELLKEKKLLPEL 420
           TGIVFEGFDRGGKLRAICGGGRYD LLSTFGGDDIPACGFGFGDAVIVELLKEKKLLPEL
Sbjct: 361 TGIVFEGFDRGGKLRAICGGGRYDHLLSTFGGDDIPACGFGFGDAVIVELLKEKKLLPEL 420

Query: 421 NVGIENIVCALDPDLQGAASTVATILREKGQTVDLVLENKPLKWVFKRATRINAQRLILV 480
           N  +ENI+CALDPDLQGAASTVATILREKGQ+VDLVLENKPLKWVFKRATRINA+RLILV
Sbjct: 421 NAEVENIICALDPDLQGAASTVATILREKGQSVDLVLENKPLKWVFKRATRINARRLILV 480

Query: 481 GSSEWQRGVVGVKDLSSGEQREVKLDELN 506
           GSSEWQRG+VGVKDLSSGEQREVKLDELN
Sbjct: 481 GSSEWQRGMVGVKDLSSGEQREVKLDELN 507

BLAST of Sed0000088 vs. NCBI nr
Match: XP_022978407.1 (histidine--tRNA ligase, chloroplastic/mitochondrial isoform X2 [Cucurbita maxima])

HSP 1 Score: 921.0 bits (2379), Expect = 4.4e-264
Identity = 469/509 (92.14%), Postives = 489/509 (96.07%), Query Frame = 0

Query: 1   MAAKPSSLLIFKPRLNLHLHLKPFIFSSSSLAFNIILRFPFPKPSFFPNRKFHSS-RAAS 60
           MAAKPSSLLIF+PRLN  L LKPFIFSS+SLAF+IILR PFPKPSFF NRKFHSS  A S
Sbjct: 1   MAAKPSSLLIFQPRLN--LRLKPFIFSSTSLAFSIILRIPFPKPSFFGNRKFHSSLSAVS 60

Query: 61  SAQSL---SGGGGRSGALAPAPATEEVQKIDVNPPKGTRDFPPEDMRLRNWLFSNFREVS 120
           SAQS+     GGGRSGAL+P   TEE+QKIDVNPPKGTRDFPPEDMRLRNWLF+NFREVS
Sbjct: 61  SAQSIGDGGNGGGRSGALSPTLVTEELQKIDVNPPKGTRDFPPEDMRLRNWLFNNFREVS 120

Query: 121 RVYGFEEVDFPVLESEALFIRKAGEEIRDQLYCFEDRGNRRVALRPELTPSLARLVIQKG 180
           RVYGFEEVDFPVLESEALFIRKAGEEIR+QLYCFEDRGNRRVALRPELTPSLARLVIQKG
Sbjct: 121 RVYGFEEVDFPVLESEALFIRKAGEEIREQLYCFEDRGNRRVALRPELTPSLARLVIQKG 180

Query: 181 KSLSLPLKWFAIGQCWRYERMTRGRRREHYQWNMDIIGVPEVTAEAELLSSIVTFFKRIG 240
           KSLSLPLKWFAIGQCWRYERMTRGRRREHYQWNMDIIGVPEVTAEAELLSSIV FFKRIG
Sbjct: 181 KSLSLPLKWFAIGQCWRYERMTRGRRREHYQWNMDIIGVPEVTAEAELLSSIVAFFKRIG 240

Query: 241 ITASDVGFKISSRKVLQEVLTCYGIPENLFGKVCIIIDKIEKIPLDEMKKELKVVGVSEE 300
           +TA+DVGFK+SSRKVLQEVL CYGIPEN+FGKVCIIIDKIEKIPLDEMKKELKVVGVSE+
Sbjct: 241 LTAADVGFKVSSRKVLQEVLKCYGIPENMFGKVCIIIDKIEKIPLDEMKKELKVVGVSED 300

Query: 301 AIEELLQVLSVKSLTKLEEILGGGGEALSDLKQLFSLAEKFGYADWIQFDASIVRGLAYY 360
           AIEE+LQVLSVKSLTKLEEILGGGGEA+SDLKQLFSLAEKFGY+DWIQFDASIVRGLAYY
Sbjct: 301 AIEEMLQVLSVKSLTKLEEILGGGGEAMSDLKQLFSLAEKFGYSDWIQFDASIVRGLAYY 360

Query: 361 TGIVFEGFDRGGKLRAICGGGRYDRLLSTFGGDDIPACGFGFGDAVIVELLKEKKLLPEL 420
           TGIVFEGFDRGGKLRAICGGGRYDRLLSTFGGDD+PACGFGFGDAVIVELLKEKKLLPEL
Sbjct: 361 TGIVFEGFDRGGKLRAICGGGRYDRLLSTFGGDDLPACGFGFGDAVIVELLKEKKLLPEL 420

Query: 421 NVGIENIVCALDPDLQGAASTVATILREKGQTVDLVLENKPLKWVFKRATRINAQRLILV 480
           +V +ENIVCALDPDLQGAAS VATILREKGQTVDLVLENKPLKWVFKRATRINAQRLILV
Sbjct: 421 SVEVENIVCALDPDLQGAASRVATILREKGQTVDLVLENKPLKWVFKRATRINAQRLILV 480

Query: 481 GSSEWQRGVVGVKDLSSGEQREVKLDELN 506
           GSSEWQRG+VGVKDLSSGEQREVKLDELN
Sbjct: 481 GSSEWQRGMVGVKDLSSGEQREVKLDELN 507

BLAST of Sed0000088 vs. NCBI nr
Match: XP_008462490.1 (PREDICTED: histidine--tRNA ligase, chloroplastic/mitochondrial [Cucumis melo] >KAA0063801.1 histidine--tRNA ligase [Cucumis melo var. makuwa])

HSP 1 Score: 920.6 bits (2378), Expect = 5.8e-264
Identity = 467/509 (91.75%), Postives = 491/509 (96.46%), Query Frame = 0

Query: 1   MAAKPSSLLIFKPRLNLHLHLKPFIFSSSSLAFNIILRFPFPKPSFFPNRKFHSS-RAAS 60
           MAAKPSSLLIF+PRLN  L LKPFIFSSSSLAF+IILRFPFPKPSFF NR F+SS  A S
Sbjct: 1   MAAKPSSLLIFQPRLN--LRLKPFIFSSSSLAFSIILRFPFPKPSFFVNRHFYSSLTAVS 60

Query: 61  SAQSLSGGG---GRSGALAPAPATEEVQKIDVNPPKGTRDFPPEDMRLRNWLFSNFREVS 120
           SAQS+S GG   GRSGAL+PAP TEE+Q+IDVNPPKGTRDFPPEDMRLRNWLF+NFREVS
Sbjct: 61  SAQSISDGGTTRGRSGALSPAPTTEELQRIDVNPPKGTRDFPPEDMRLRNWLFNNFREVS 120

Query: 121 RVYGFEEVDFPVLESEALFIRKAGEEIRDQLYCFEDRGNRRVALRPELTPSLARLVIQKG 180
           R+YGFEEVD+PVLESEALFIRKAGEEIRDQLYCFEDRGNRRVALRPELTPSLARLVIQKG
Sbjct: 121 RLYGFEEVDYPVLESEALFIRKAGEEIRDQLYCFEDRGNRRVALRPELTPSLARLVIQKG 180

Query: 181 KSLSLPLKWFAIGQCWRYERMTRGRRREHYQWNMDIIGVPEVTAEAELLSSIVTFFKRIG 240
           KSLSLP+KWFAIGQCWRYERMTRGRRREHYQWNMDI+GVPEVTAEAELLSSIVTFFKR+G
Sbjct: 181 KSLSLPIKWFAIGQCWRYERMTRGRRREHYQWNMDILGVPEVTAEAELLSSIVTFFKRVG 240

Query: 241 ITASDVGFKISSRKVLQEVLTCYGIPENLFGKVCIIIDKIEKIPLDEMKKELKVVGVSEE 300
           ITASDVGFK+SSRKVLQ+VL CYGIP+N+FGKVCIIIDKIEKIPLDEMKKELKVVGVSE+
Sbjct: 241 ITASDVGFKVSSRKVLQDVLKCYGIPQNMFGKVCIIIDKIEKIPLDEMKKELKVVGVSED 300

Query: 301 AIEELLQVLSVKSLTKLEEILGGGGEALSDLKQLFSLAEKFGYADWIQFDASIVRGLAYY 360
           AIEELLQVLSVKSLTKLEE+LGGGGEALSDLKQLFSLAEKFGY+DWIQFDASIVRGLAYY
Sbjct: 301 AIEELLQVLSVKSLTKLEEVLGGGGEALSDLKQLFSLAEKFGYSDWIQFDASIVRGLAYY 360

Query: 361 TGIVFEGFDRGGKLRAICGGGRYDRLLSTFGGDDIPACGFGFGDAVIVELLKEKKLLPEL 420
           TGIVFEGFDRGGKLRAICGGGRYDRLLSTFGGDDIPACGFGFGDAVIVELLKEKKLLPEL
Sbjct: 361 TGIVFEGFDRGGKLRAICGGGRYDRLLSTFGGDDIPACGFGFGDAVIVELLKEKKLLPEL 420

Query: 421 NVGIENIVCALDPDLQGAASTVATILREKGQTVDLVLENKPLKWVFKRATRINAQRLILV 480
           N  +ENIVCALDPDLQGAAS VATILREKGQTVDLVLENKPLKWVFKRATRINA+RLILV
Sbjct: 421 NAEVENIVCALDPDLQGAASKVATILREKGQTVDLVLENKPLKWVFKRATRINARRLILV 480

Query: 481 GSSEWQRGVVGVKDLSSGEQREVKLDELN 506
           GSSEW+RG+VGVKDLSSGEQ EVKLDELN
Sbjct: 481 GSSEWERGMVGVKDLSSGEQCEVKLDELN 507

BLAST of Sed0000088 vs. NCBI nr
Match: TYK07323.1 (histidine--tRNA ligase [Cucumis melo var. makuwa])

HSP 1 Score: 920.2 bits (2377), Expect = 7.6e-264
Identity = 466/509 (91.55%), Postives = 491/509 (96.46%), Query Frame = 0

Query: 1   MAAKPSSLLIFKPRLNLHLHLKPFIFSSSSLAFNIILRFPFPKPSFFPNRKFHSS-RAAS 60
           MAAKPSSLLIF+PRLN  L LKPFIFSSSSLAF+IILRFPFPKPSFF NR F+SS  A S
Sbjct: 1   MAAKPSSLLIFQPRLN--LRLKPFIFSSSSLAFSIILRFPFPKPSFFVNRHFYSSLTAVS 60

Query: 61  SAQSLSGGG---GRSGALAPAPATEEVQKIDVNPPKGTRDFPPEDMRLRNWLFSNFREVS 120
           SAQS+S GG   GRSGAL+PAP TEE+Q+IDVNPPKGTRDFPPEDMRLRNWLF+NFREVS
Sbjct: 61  SAQSISDGGTTRGRSGALSPAPTTEELQRIDVNPPKGTRDFPPEDMRLRNWLFNNFREVS 120

Query: 121 RVYGFEEVDFPVLESEALFIRKAGEEIRDQLYCFEDRGNRRVALRPELTPSLARLVIQKG 180
           R+YGFEEVD+PVLESEALFIRKAGEEIRDQLYCFEDRGNRRVALRPELTPSLARLVIQKG
Sbjct: 121 RLYGFEEVDYPVLESEALFIRKAGEEIRDQLYCFEDRGNRRVALRPELTPSLARLVIQKG 180

Query: 181 KSLSLPLKWFAIGQCWRYERMTRGRRREHYQWNMDIIGVPEVTAEAELLSSIVTFFKRIG 240
           KSLSLP+KWFAIGQCWRYERMTRGRRREHYQWNMDI+GVPEVTAEAELLSSIVTFFKR+G
Sbjct: 181 KSLSLPIKWFAIGQCWRYERMTRGRRREHYQWNMDILGVPEVTAEAELLSSIVTFFKRVG 240

Query: 241 ITASDVGFKISSRKVLQEVLTCYGIPENLFGKVCIIIDKIEKIPLDEMKKELKVVGVSEE 300
           ITASDVGFK+SSRKVLQ+VL CYGIP+N+FGKVCIIIDKIEKIPLDEMKKELKVVGVSE+
Sbjct: 241 ITASDVGFKVSSRKVLQDVLKCYGIPQNMFGKVCIIIDKIEKIPLDEMKKELKVVGVSED 300

Query: 301 AIEELLQVLSVKSLTKLEEILGGGGEALSDLKQLFSLAEKFGYADWIQFDASIVRGLAYY 360
           AIEELLQVLSVKSLTKLEE+LGGGGEALSDLKQLFSLAEKFGY+DWIQFDASIVRGLAYY
Sbjct: 301 AIEELLQVLSVKSLTKLEEVLGGGGEALSDLKQLFSLAEKFGYSDWIQFDASIVRGLAYY 360

Query: 361 TGIVFEGFDRGGKLRAICGGGRYDRLLSTFGGDDIPACGFGFGDAVIVELLKEKKLLPEL 420
           TGIVFEGFDRGGKLRAICGGGRYDRLLSTFGGDDIPACGFGFGDAVIVELLKEKKLLPEL
Sbjct: 361 TGIVFEGFDRGGKLRAICGGGRYDRLLSTFGGDDIPACGFGFGDAVIVELLKEKKLLPEL 420

Query: 421 NVGIENIVCALDPDLQGAASTVATILREKGQTVDLVLENKPLKWVFKRATRINAQRLILV 480
           N  +ENIVCALDPDLQGAAS VAT+LREKGQTVDLVLENKPLKWVFKRATRINA+RLILV
Sbjct: 421 NAEVENIVCALDPDLQGAASKVATVLREKGQTVDLVLENKPLKWVFKRATRINARRLILV 480

Query: 481 GSSEWQRGVVGVKDLSSGEQREVKLDELN 506
           GSSEW+RG+VGVKDLSSGEQ EVKLDELN
Sbjct: 481 GSSEWERGMVGVKDLSSGEQCEVKLDELN 507

BLAST of Sed0000088 vs. NCBI nr
Match: XP_023544233.1 (histidine--tRNA ligase, chloroplastic/mitochondrial isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 919.5 bits (2375), Expect = 1.3e-263
Identity = 470/509 (92.34%), Postives = 486/509 (95.48%), Query Frame = 0

Query: 1   MAAKPSSLLIFKPRLNLHLHLKPFIFSSSSLAFNIILRFPFPKPSFFPNRKFHSS-RAAS 60
           MAAKPSSLLIF+PRLN  L LKPFIFSSSSLAF+IILR PFPK SFF NRKFHSS  A S
Sbjct: 1   MAAKPSSLLIFQPRLN--LRLKPFIFSSSSLAFSIILRIPFPKASFFGNRKFHSSLSAVS 60

Query: 61  SAQSL---SGGGGRSGALAPAPATEEVQKIDVNPPKGTRDFPPEDMRLRNWLFSNFREVS 120
           SAQS      GGGRSGAL+P    EE+QKIDVNPPKGTRDFPPEDMRLRNWLF+NFREVS
Sbjct: 61  SAQSTGDGGNGGGRSGALSPTLVAEELQKIDVNPPKGTRDFPPEDMRLRNWLFNNFREVS 120

Query: 121 RVYGFEEVDFPVLESEALFIRKAGEEIRDQLYCFEDRGNRRVALRPELTPSLARLVIQKG 180
           RVYGFEEVDFPVLESEALFIRKAGEEIRDQLYCFEDRGNRRVALRPELTPSLARLVIQKG
Sbjct: 121 RVYGFEEVDFPVLESEALFIRKAGEEIRDQLYCFEDRGNRRVALRPELTPSLARLVIQKG 180

Query: 181 KSLSLPLKWFAIGQCWRYERMTRGRRREHYQWNMDIIGVPEVTAEAELLSSIVTFFKRIG 240
           KSLSLPLKWFAIGQCWRYERMTRGRRREHYQWNMDIIGVPEVTAEAELLSSIV FFKRIG
Sbjct: 181 KSLSLPLKWFAIGQCWRYERMTRGRRREHYQWNMDIIGVPEVTAEAELLSSIVAFFKRIG 240

Query: 241 ITASDVGFKISSRKVLQEVLTCYGIPENLFGKVCIIIDKIEKIPLDEMKKELKVVGVSEE 300
           +TA+DVGFK+SSRKVLQEVL CYGIPEN+FGKVCIIIDKIEKIPLDEMKKELKVVGVSE+
Sbjct: 241 LTAADVGFKVSSRKVLQEVLKCYGIPENMFGKVCIIIDKIEKIPLDEMKKELKVVGVSED 300

Query: 301 AIEELLQVLSVKSLTKLEEILGGGGEALSDLKQLFSLAEKFGYADWIQFDASIVRGLAYY 360
           AIEE+LQVLSVKSLTKLEEILGGGGEA+SDLKQLFSLAEKFGY+DWIQFDASIVRGLAYY
Sbjct: 301 AIEEMLQVLSVKSLTKLEEILGGGGEAMSDLKQLFSLAEKFGYSDWIQFDASIVRGLAYY 360

Query: 361 TGIVFEGFDRGGKLRAICGGGRYDRLLSTFGGDDIPACGFGFGDAVIVELLKEKKLLPEL 420
           TGIVFEGFDRGGKLRAICGGGRYDRLLSTFGGDD+PACGFGFGDAVIVELLKEKKLLPEL
Sbjct: 361 TGIVFEGFDRGGKLRAICGGGRYDRLLSTFGGDDLPACGFGFGDAVIVELLKEKKLLPEL 420

Query: 421 NVGIENIVCALDPDLQGAASTVATILREKGQTVDLVLENKPLKWVFKRATRINAQRLILV 480
           NV +ENIVCALDPDLQGAAS VATILREKGQTVDLVLENKPLKWVFKRATRINAQRLILV
Sbjct: 421 NVEVENIVCALDPDLQGAASRVATILREKGQTVDLVLENKPLKWVFKRATRINAQRLILV 480

Query: 481 GSSEWQRGVVGVKDLSSGEQREVKLDELN 506
           GSSEWQRG+VGVKDLSSGEQREVKLDELN
Sbjct: 481 GSSEWQRGMVGVKDLSSGEQREVKLDELN 507

BLAST of Sed0000088 vs. ExPASy Swiss-Prot
Match: O82413 (Histidine--tRNA ligase, chloroplastic/mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At3g46100 PE=2 SV=1)

HSP 1 Score: 744.2 bits (1920), Expect = 9.7e-214
Identity = 371/459 (80.83%), Postives = 415/459 (90.41%), Query Frame = 0

Query: 48  PNRKFHSSRAASSAQSLSGGGGRSGALAPAPATEE--VQKIDVNPPKGTRDFPPEDMRLR 107
           P R+F   R    A   + GGGRSG++     TEE   +KIDVNPPKGTRDFPPEDMRLR
Sbjct: 29  PPRQFSIPRRLICA--AANGGGRSGSIVAPLVTEEDFHKKIDVNPPKGTRDFPPEDMRLR 88

Query: 108 NWLFSNFREVSRVYGFEEVDFPVLESEALFIRKAGEEIRDQLYCFEDRGNRRVALRPELT 167
           NWLF++F+EVSR+YG+EEVD+PVLE+EALFIRKAGEEIRDQLYCFEDRGNRRVALRPELT
Sbjct: 89  NWLFNHFKEVSRLYGYEEVDYPVLETEALFIRKAGEEIRDQLYCFEDRGNRRVALRPELT 148

Query: 168 PSLARLVIQKGKSLSLPLKWFAIGQCWRYERMTRGRRREHYQWNMDIIGVPEVTAEAELL 227
           PSLARLVIQKGKS+SLPLKWFAIGQCWRYERMTRGRRREHYQWNMDIIGVP+VTAEAEL+
Sbjct: 149 PSLARLVIQKGKSVSLPLKWFAIGQCWRYERMTRGRRREHYQWNMDIIGVPQVTAEAELI 208

Query: 228 SSIVTFFKRIGITASDVGFKISSRKVLQEVLTCYGIPENLFGKVCIIIDKIEKIPLDEMK 287
           SSIVTFFKRIGITASDVGFK+SSRKVLQE+L  YG+PE+LFG+VCIIIDKIEKIP+DE+K
Sbjct: 209 SSIVTFFKRIGITASDVGFKVSSRKVLQELLKKYGVPEDLFGRVCIIIDKIEKIPIDEIK 268

Query: 288 KELKVVGVSEEAIEELLQVLSVKSLTKLEEILGGGGEALSDLKQLFSLAEKFGYADWIQF 347
           KEL   G+SE+AIE+LLQVLSVKSL  LE+I+GG GEA++DLKQLFSLAEKFGY++WIQF
Sbjct: 269 KELGFTGISEDAIEQLLQVLSVKSLDDLEDIIGGAGEAIADLKQLFSLAEKFGYSEWIQF 328

Query: 348 DASIVRGLAYYTGIVFEGFDRGGKLRAICGGGRYDRLLSTFGGDDIPACGFGFGDAVIVE 407
           DAS+VRGLAYYTGIVFEGFDR GKLRAICGGGRYDRLLST+GGDD PACGFGFGDAVIVE
Sbjct: 329 DASVVRGLAYYTGIVFEGFDRKGKLRAICGGGRYDRLLSTYGGDDFPACGFGFGDAVIVE 388

Query: 408 LLKEKKLLPELNVGIENIVCALDPDLQGAASTVATILREKGQTVDLVLENKPLKWVFKRA 467
           LLKEK LLPEL   +ENIVCALD DLQGAA+TVAT LR+KGQTVDLVLE+KPLKWVFKRA
Sbjct: 389 LLKEKDLLPELGQEVENIVCALDKDLQGAAATVATALRDKGQTVDLVLESKPLKWVFKRA 448

Query: 468 TRINAQRLILVGSSEWQRGVVGVKDLSSGEQREVKLDEL 505
            R+NA+RL+LVG +EW+ G V VK LSSGEQ +VKL +L
Sbjct: 449 ARVNARRLVLVGKTEWEDGSVSVKVLSSGEQFQVKLSDL 485

BLAST of Sed0000088 vs. ExPASy Swiss-Prot
Match: Q8U431 (Histidine--tRNA ligase OS=Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1) OX=186497 GN=hisS PE=3 SV=1)

HSP 1 Score: 268.5 bits (685), Expect = 1.6e-70
Identity = 153/423 (36.17%), Postives = 242/423 (57.21%), Query Frame = 0

Query: 92  KGTRDFPPEDMRLRNWLFSNFREVSRVYGFEEVDFPVLESEALFIRKAGEEIRDQLYCFE 151
           KGTRDF PE+M  R W+F   RE    YGF+EV  P +E   LF  ++GEE+  QLY F+
Sbjct: 6   KGTRDFLPEEMVKRRWVFERIRERFETYGFKEVLTPTMEYTKLFELRSGEEVVKQLYAFK 65

Query: 152 DRGNRRVALRPELTPSLARLVIQKGKSLSLPLKWFAIGQCWRYERMTRGRRREHYQWNMD 211
           D+G R V+LRP++T S+ARL +   ++   P+KW+ I   +RYE    GR RE +Q  ++
Sbjct: 66  DKGGRDVSLRPDMTSSVARLYVNMFQTAPKPIKWYYIANMFRYEEPQSGRYREFWQAGVE 125

Query: 212 IIGVPEVTAEAELLSSIVTFFKRIGITASDVGFKISSRKVLQEVLTCYGIPENLFGKVCI 271
           +IG  +V A+AE+++  V  +   G+    V   I  R +L E     G+ +++   +  
Sbjct: 126 LIGSDKVEADAEVIALFVESYLATGLREFTV--NIGDRILLDEFAKMLGVKDDI--GLMR 185

Query: 272 IIDKIEKIPLDEMKKELKVVGVSEEAIEELLQVLSVK----SLTKLEEILGGGGEALSDL 331
           IIDK +K+P ++    LK  G+ +  IE++L+++++K     +  L E L     A +++
Sbjct: 186 IIDKKDKLPQEDFINALKEFGLDKNGIEKVLELINIKGKPDEVLPLAEELFTSEVAKNEI 245

Query: 332 KQLFS---LAEKFGYADWIQFDASIVRGLAYYTGIVFEGF---DRGGKLRAICGGGRYDR 391
            +L++   L E +   DWI+ D  I RG  YYT IVFE     D G  + +I GGGRYD 
Sbjct: 246 NRLYALIDLLEAYEVKDWIRIDLGIARGFDYYTSIVFEAIAPNDLG--IGSIGGGGRYDN 305

Query: 392 LLSTFGGDDIPACGFGFGDAVIVELLKEKKLLPELNVGIENIVCALDPDLQGAASTVATI 451
           L+  FGG   PA GF  G   ++ +L+ K L+PE+ +  +  V  +   ++  A  +AT 
Sbjct: 306 LIEVFGGKPTPATGFAIGIERLIPILEAKGLIPEVQIAPDVYVVPIGERMKKVAIRIATS 365

Query: 452 LREKGQTVDLVLENKPLKWVFKRATRINAQRLILVGSSEWQRGVVGVKDLSSGEQREVKL 505
           LR+ G   ++ L  + LK     A ++  ++ I+VG  + +RGVV V+D+ SGEQ EVK+
Sbjct: 366 LRKSGIKTEIELMGRKLKKAMDYANKVGVKKTIIVGEKDLERGVVTVRDMESGEQNEVKI 422

BLAST of Sed0000088 vs. ExPASy Swiss-Prot
Match: Q9HLX5 (Histidine--tRNA ligase OS=Thermoplasma acidophilum (strain ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165) OX=273075 GN=hisS PE=1 SV=1)

HSP 1 Score: 267.7 bits (683), Expect = 2.7e-70
Identity = 150/423 (35.46%), Postives = 238/423 (56.26%), Query Frame = 0

Query: 85  KIDVNPPKGTRDFPPEDMRLRNWLFSNFREVSRVYGFEEVDFPVLESEALFIRKAGEEIR 144
           ++ +   +G RDF PEDM +  ++F    E +  +GF  +DFP LE   L+  K+GEE+ 
Sbjct: 3   RLQIEKIRGFRDFYPEDMDVEKFIFKTAEEAAEAFGFRRIDFPSLEYLDLYRIKSGEELL 62

Query: 145 DQLYCFEDRGNRRVALRPELTPSLARLVIQKGKSLSLPLKWFAIGQCWRYERMTRGRRRE 204
            Q Y F D+G R V L PE TPS  R+V  + K L  PL+W++  + WRYE    GR RE
Sbjct: 63  QQTYSFVDKGGREVTLIPEATPSTVRMVTSR-KDLQRPLRWYSFPKVWRYEEPQAGRYRE 122

Query: 205 HYQWNMDIIGVPEVTAEAELLSSIVTFFKRIGITASDV-GFKISSRKVLQEVLTCYGIPE 264
           HYQ+N DI G     A+AE+++   +   R+G+   D+   +I+SRK+++E++   G+  
Sbjct: 123 HYQFNADIFGSDSPEADAEVIALASSILDRLGL--QDIYEIRINSRKIMEEIIG--GMTS 182

Query: 265 NLFGKVCIIIDKIEKIPLDEMKKELKVVGVSEEAIEELLQVLS-VKSLTKLEEILGGGGE 324
           +    V  IID+  KI  +E   +L+  G+ E+ +  +  + S  + + ++  I G   E
Sbjct: 183 SDPFSVFSIIDRYHKISREEFVDQLRSAGIGEDGVSMIADLCSGTRGIDEMARITGKSSE 242

Query: 325 ALSDLKQLFSLAEKFGYADWIQFDASIVRGLAYYTGIVFEGFDRGGKLRAICGGGRYDRL 384
            ++ +  +  L   +G  + +++D SIVRGL+YYTGIVFE +DR G+ RAI GGGRYD L
Sbjct: 243 EIARMAAVEDLLASYGVKN-VRYDFSIVRGLSYYTGIVFEAYDRSGQFRAILGGGRYDNL 302

Query: 385 LSTFGGDDIPACGFGFGDAVIVELLKEKKL-LPELNVGIENIVCALDPDLQGAASTVATI 444
            S   G+ +PA GFG GDAVI  LLK + + +P     +   +C +        +  +  
Sbjct: 303 ASLMSGESVPAVGFGMGDAVISLLLKRENVQIPREKKSV--YICRVGKINSSIMNEYSRK 362

Query: 445 LREKGQTVDLVLENKPLKWVFKRATRINAQRLILVGSSEWQRGVVGVKDLSSGEQREVKL 504
           LRE+G  V + +  + L    K A+ I A   ++ G  + +RGVV ++++ +G Q  V L
Sbjct: 363 LRERGMNVTVEIMERGLSAQLKYASAIGADFAVIFGERDLERGVVTIRNMYTGSQENVGL 417

BLAST of Sed0000088 vs. ExPASy Swiss-Prot
Match: O58028 (Histidine--tRNA ligase OS=Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3) OX=70601 GN=hisS PE=3 SV=1)

HSP 1 Score: 265.4 bits (677), Expect = 1.3e-69
Identity = 158/421 (37.53%), Postives = 234/421 (55.58%), Query Frame = 0

Query: 92  KGTRDFPPEDMRLRNWLFSNFREVSRVYGFEEVDFPVLESEALFIRKAGEEIRDQLYCFE 151
           KGTRDF PEDM  R W+F   REV   YGF+E+  PV+E   LF  ++GEE+  QLY F+
Sbjct: 6   KGTRDFLPEDMAKRRWVFEKIREVFEAYGFKEILTPVMEYTKLFQLRSGEEVVKQLYAFK 65

Query: 152 DRGNRRVALRPELTPSLARLVIQKGKSLSLPLKWFAIGQCWRYERMTRGRRREHYQWNMD 211
           D+G R V+LRP++T S+ARL +   +    P+KW+ I   +RYE    GR RE +Q  ++
Sbjct: 66  DKGGRDVSLRPDMTSSVARLYVNSFQMAPKPIKWYYIANMFRYEEPQSGRYREFWQAGVE 125

Query: 212 IIGVPEVTAEAELLSSIVTFFKRIGITASDVGFKISSRKVLQEVLTCYGIPENLFGKVCI 271
           +IG  +V A+AE+++  V  +   G+   D    I  R +L E     G+ +++   +  
Sbjct: 126 LIGSDKVEADAEVIALFVESYLSTGL--RDFTVNIGDRILLDEFAGMLGVSDDI--GLMR 185

Query: 272 IIDKIEKIPLDEMKKELKVVGVSEEAIEELLQVLSVKS-----LTKLEEILGG--GGEAL 331
           IIDK +K+  +E    LK  G+ E  IE++L+++ +K      L   EE+       E +
Sbjct: 186 IIDKRDKLSQEEFINLLKEFGLGESEIEKVLELVEIKGRPDNVLPLAEELFKSKKAKEEI 245

Query: 332 SDLKQLFSLAEKFGYADWIQFDASIVRGLAYYTGIVFEGFDRGG-KLRAICGGGRYDRLL 391
           S L +L  L E +G  DWI+ D  I RG  YYT IVFE        + +I GGGRYD L+
Sbjct: 246 SKLYKLTDLLEWYGVKDWIRIDLGIARGFDYYTSIVFEAISPNELGIGSIGGGGRYDNLI 305

Query: 392 STFGGDDIPACGFGFGDAVIVELLKEKKLLPELNVGIENIVCALDPDLQGAASTVATILR 451
             FGG   PA GF  G   ++ +L+ K L+P+   G E  V  L  D++  A  +A  LR
Sbjct: 306 EIFGGKPTPATGFAIGIERLLPILEWKGLIPKPQTGPEVFVIPL-KDMEKVAINIAVKLR 365

Query: 452 EKGQTVDLVLENKPLKWVFKRATRINAQRLILVGSSEWQRGVVGVKDLSSGEQREVKLDE 505
            +    D+ L  + L      A R+ A+ +I+VG  + +RGVV ++D+ SGEQ  V L+E
Sbjct: 366 REKIKTDIELSGRKLGKALDYANRVGAKLVIIVGKRDVERGVVTIRDMESGEQYNVSLNE 421

BLAST of Sed0000088 vs. ExPASy Swiss-Prot
Match: Q9UY31 (Histidine--tRNA ligase OS=Pyrococcus abyssi (strain GE5 / Orsay) OX=272844 GN=hisS PE=3 SV=1)

HSP 1 Score: 265.0 bits (676), Expect = 1.7e-69
Identity = 158/423 (37.35%), Postives = 236/423 (55.79%), Query Frame = 0

Query: 92  KGTRDFPPEDMRLRNWLFSNFREVSRVYGFEEVDFPVLESEALFIRKAGEEIRDQLYCFE 151
           KGTRDF PE+M  R W+F   REV   YGF+EV  PV+E   LF  ++GEE+  QLY F+
Sbjct: 6   KGTRDFLPEEMVKRRWVFEKIREVFETYGFKEVLTPVMEYTKLFQLRSGEEVVKQLYAFK 65

Query: 152 DRGNRRVALRPELTPSLARLVIQKGKSLSLPLKWFAIGQCWRYERMTRGRRREHYQWNMD 211
           D+G R VALRP++T S+ARL +   ++   P+KW+ I   +RYE    GR RE +Q  ++
Sbjct: 66  DKGGRDVALRPDMTSSVARLYVNSFQTAPKPIKWYYIANMFRYEEPQSGRYREFWQAGVE 125

Query: 212 IIGVPEVTAEAELLSSIVTFFKRIGITASDVGFKISSRKVLQEVLTCYGIPENLFGKVCI 271
           +IG  ++ A+AE+++  V  +   G+   D    I  R +L E     G+ +++   +  
Sbjct: 126 LIGSDKIEADAEVIALFVDSYLSTGL--KDFTVNIGDRVLLDEFAKMLGVKDDI--GLMR 185

Query: 272 IIDKIEKIPLDEMKKELKVVGVSEEAIEELLQVLSVK----SLTKLEEILGGGGEALSDL 331
           IIDK +K+  +E  K L   G+ E  IE++L ++ +K     +  L E L     A  ++
Sbjct: 186 IIDKKDKLSQEEFLKALGEFGLDENGIEKVLNLIEIKGKPDDVLPLAEELFTSERAKEEI 245

Query: 332 KQLFSLAEKFGY---ADWIQFDASIVRGLAYYTGIVFEGF---DRGGKLRAICGGGRYDR 391
            +L++L +   +    +WIQ D  I RG  YYT IVFE     D G  + +I GGGRYD 
Sbjct: 246 SRLYNLVDILSWYEVDEWIQIDLGIARGFDYYTSIVFEAIVPNDLG--IGSIGGGGRYDN 305

Query: 392 LLSTFGGDDIPACGFGFGDAVIVELLKEKKLLPELNVGIENIVCALDPDLQGAASTVATI 451
           L+  FGG   PA GF  G   ++ +L+ K LLPEL  G +  V  +  D +  A+ + T 
Sbjct: 306 LIEVFGGKPTPATGFAIGIERLIPILEWKGLLPELKAGPDVFVIPVG-DSRDVATAIVTR 365

Query: 452 LREKGQTVDLVLENKPLKWVFKRATRINAQRLILVGSSEWQRGVVGVKDLSSGEQREVKL 505
           LR+ G   D+ L  + L+     A RI  +  I+VG  + +RGVV ++DL SG Q EV +
Sbjct: 366 LRKAGIRSDIELSGRKLRKALDYANRIGVRLSIIVGKRDLERGVVTIRDLESGNQVEVPV 421

BLAST of Sed0000088 vs. ExPASy TrEMBL
Match: A0A6J1IL10 (Histidine--tRNA ligase OS=Cucurbita maxima OX=3661 GN=LOC111478405 PE=3 SV=1)

HSP 1 Score: 921.0 bits (2379), Expect = 2.1e-264
Identity = 469/509 (92.14%), Postives = 489/509 (96.07%), Query Frame = 0

Query: 1   MAAKPSSLLIFKPRLNLHLHLKPFIFSSSSLAFNIILRFPFPKPSFFPNRKFHSS-RAAS 60
           MAAKPSSLLIF+PRLN  L LKPFIFSS+SLAF+IILR PFPKPSFF NRKFHSS  A S
Sbjct: 1   MAAKPSSLLIFQPRLN--LRLKPFIFSSTSLAFSIILRIPFPKPSFFGNRKFHSSLSAVS 60

Query: 61  SAQSL---SGGGGRSGALAPAPATEEVQKIDVNPPKGTRDFPPEDMRLRNWLFSNFREVS 120
           SAQS+     GGGRSGAL+P   TEE+QKIDVNPPKGTRDFPPEDMRLRNWLF+NFREVS
Sbjct: 61  SAQSIGDGGNGGGRSGALSPTLVTEELQKIDVNPPKGTRDFPPEDMRLRNWLFNNFREVS 120

Query: 121 RVYGFEEVDFPVLESEALFIRKAGEEIRDQLYCFEDRGNRRVALRPELTPSLARLVIQKG 180
           RVYGFEEVDFPVLESEALFIRKAGEEIR+QLYCFEDRGNRRVALRPELTPSLARLVIQKG
Sbjct: 121 RVYGFEEVDFPVLESEALFIRKAGEEIREQLYCFEDRGNRRVALRPELTPSLARLVIQKG 180

Query: 181 KSLSLPLKWFAIGQCWRYERMTRGRRREHYQWNMDIIGVPEVTAEAELLSSIVTFFKRIG 240
           KSLSLPLKWFAIGQCWRYERMTRGRRREHYQWNMDIIGVPEVTAEAELLSSIV FFKRIG
Sbjct: 181 KSLSLPLKWFAIGQCWRYERMTRGRRREHYQWNMDIIGVPEVTAEAELLSSIVAFFKRIG 240

Query: 241 ITASDVGFKISSRKVLQEVLTCYGIPENLFGKVCIIIDKIEKIPLDEMKKELKVVGVSEE 300
           +TA+DVGFK+SSRKVLQEVL CYGIPEN+FGKVCIIIDKIEKIPLDEMKKELKVVGVSE+
Sbjct: 241 LTAADVGFKVSSRKVLQEVLKCYGIPENMFGKVCIIIDKIEKIPLDEMKKELKVVGVSED 300

Query: 301 AIEELLQVLSVKSLTKLEEILGGGGEALSDLKQLFSLAEKFGYADWIQFDASIVRGLAYY 360
           AIEE+LQVLSVKSLTKLEEILGGGGEA+SDLKQLFSLAEKFGY+DWIQFDASIVRGLAYY
Sbjct: 301 AIEEMLQVLSVKSLTKLEEILGGGGEAMSDLKQLFSLAEKFGYSDWIQFDASIVRGLAYY 360

Query: 361 TGIVFEGFDRGGKLRAICGGGRYDRLLSTFGGDDIPACGFGFGDAVIVELLKEKKLLPEL 420
           TGIVFEGFDRGGKLRAICGGGRYDRLLSTFGGDD+PACGFGFGDAVIVELLKEKKLLPEL
Sbjct: 361 TGIVFEGFDRGGKLRAICGGGRYDRLLSTFGGDDLPACGFGFGDAVIVELLKEKKLLPEL 420

Query: 421 NVGIENIVCALDPDLQGAASTVATILREKGQTVDLVLENKPLKWVFKRATRINAQRLILV 480
           +V +ENIVCALDPDLQGAAS VATILREKGQTVDLVLENKPLKWVFKRATRINAQRLILV
Sbjct: 421 SVEVENIVCALDPDLQGAASRVATILREKGQTVDLVLENKPLKWVFKRATRINAQRLILV 480

Query: 481 GSSEWQRGVVGVKDLSSGEQREVKLDELN 506
           GSSEWQRG+VGVKDLSSGEQREVKLDELN
Sbjct: 481 GSSEWQRGMVGVKDLSSGEQREVKLDELN 507

BLAST of Sed0000088 vs. ExPASy TrEMBL
Match: A0A5A7VDI2 (Histidine--tRNA ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold827G00160 PE=3 SV=1)

HSP 1 Score: 920.6 bits (2378), Expect = 2.8e-264
Identity = 467/509 (91.75%), Postives = 491/509 (96.46%), Query Frame = 0

Query: 1   MAAKPSSLLIFKPRLNLHLHLKPFIFSSSSLAFNIILRFPFPKPSFFPNRKFHSS-RAAS 60
           MAAKPSSLLIF+PRLN  L LKPFIFSSSSLAF+IILRFPFPKPSFF NR F+SS  A S
Sbjct: 1   MAAKPSSLLIFQPRLN--LRLKPFIFSSSSLAFSIILRFPFPKPSFFVNRHFYSSLTAVS 60

Query: 61  SAQSLSGGG---GRSGALAPAPATEEVQKIDVNPPKGTRDFPPEDMRLRNWLFSNFREVS 120
           SAQS+S GG   GRSGAL+PAP TEE+Q+IDVNPPKGTRDFPPEDMRLRNWLF+NFREVS
Sbjct: 61  SAQSISDGGTTRGRSGALSPAPTTEELQRIDVNPPKGTRDFPPEDMRLRNWLFNNFREVS 120

Query: 121 RVYGFEEVDFPVLESEALFIRKAGEEIRDQLYCFEDRGNRRVALRPELTPSLARLVIQKG 180
           R+YGFEEVD+PVLESEALFIRKAGEEIRDQLYCFEDRGNRRVALRPELTPSLARLVIQKG
Sbjct: 121 RLYGFEEVDYPVLESEALFIRKAGEEIRDQLYCFEDRGNRRVALRPELTPSLARLVIQKG 180

Query: 181 KSLSLPLKWFAIGQCWRYERMTRGRRREHYQWNMDIIGVPEVTAEAELLSSIVTFFKRIG 240
           KSLSLP+KWFAIGQCWRYERMTRGRRREHYQWNMDI+GVPEVTAEAELLSSIVTFFKR+G
Sbjct: 181 KSLSLPIKWFAIGQCWRYERMTRGRRREHYQWNMDILGVPEVTAEAELLSSIVTFFKRVG 240

Query: 241 ITASDVGFKISSRKVLQEVLTCYGIPENLFGKVCIIIDKIEKIPLDEMKKELKVVGVSEE 300
           ITASDVGFK+SSRKVLQ+VL CYGIP+N+FGKVCIIIDKIEKIPLDEMKKELKVVGVSE+
Sbjct: 241 ITASDVGFKVSSRKVLQDVLKCYGIPQNMFGKVCIIIDKIEKIPLDEMKKELKVVGVSED 300

Query: 301 AIEELLQVLSVKSLTKLEEILGGGGEALSDLKQLFSLAEKFGYADWIQFDASIVRGLAYY 360
           AIEELLQVLSVKSLTKLEE+LGGGGEALSDLKQLFSLAEKFGY+DWIQFDASIVRGLAYY
Sbjct: 301 AIEELLQVLSVKSLTKLEEVLGGGGEALSDLKQLFSLAEKFGYSDWIQFDASIVRGLAYY 360

Query: 361 TGIVFEGFDRGGKLRAICGGGRYDRLLSTFGGDDIPACGFGFGDAVIVELLKEKKLLPEL 420
           TGIVFEGFDRGGKLRAICGGGRYDRLLSTFGGDDIPACGFGFGDAVIVELLKEKKLLPEL
Sbjct: 361 TGIVFEGFDRGGKLRAICGGGRYDRLLSTFGGDDIPACGFGFGDAVIVELLKEKKLLPEL 420

Query: 421 NVGIENIVCALDPDLQGAASTVATILREKGQTVDLVLENKPLKWVFKRATRINAQRLILV 480
           N  +ENIVCALDPDLQGAAS VATILREKGQTVDLVLENKPLKWVFKRATRINA+RLILV
Sbjct: 421 NAEVENIVCALDPDLQGAASKVATILREKGQTVDLVLENKPLKWVFKRATRINARRLILV 480

Query: 481 GSSEWQRGVVGVKDLSSGEQREVKLDELN 506
           GSSEW+RG+VGVKDLSSGEQ EVKLDELN
Sbjct: 481 GSSEWERGMVGVKDLSSGEQCEVKLDELN 507

BLAST of Sed0000088 vs. ExPASy TrEMBL
Match: A0A1S3CHK2 (Histidine--tRNA ligase OS=Cucumis melo OX=3656 GN=LOC103500830 PE=3 SV=1)

HSP 1 Score: 920.6 bits (2378), Expect = 2.8e-264
Identity = 467/509 (91.75%), Postives = 491/509 (96.46%), Query Frame = 0

Query: 1   MAAKPSSLLIFKPRLNLHLHLKPFIFSSSSLAFNIILRFPFPKPSFFPNRKFHSS-RAAS 60
           MAAKPSSLLIF+PRLN  L LKPFIFSSSSLAF+IILRFPFPKPSFF NR F+SS  A S
Sbjct: 1   MAAKPSSLLIFQPRLN--LRLKPFIFSSSSLAFSIILRFPFPKPSFFVNRHFYSSLTAVS 60

Query: 61  SAQSLSGGG---GRSGALAPAPATEEVQKIDVNPPKGTRDFPPEDMRLRNWLFSNFREVS 120
           SAQS+S GG   GRSGAL+PAP TEE+Q+IDVNPPKGTRDFPPEDMRLRNWLF+NFREVS
Sbjct: 61  SAQSISDGGTTRGRSGALSPAPTTEELQRIDVNPPKGTRDFPPEDMRLRNWLFNNFREVS 120

Query: 121 RVYGFEEVDFPVLESEALFIRKAGEEIRDQLYCFEDRGNRRVALRPELTPSLARLVIQKG 180
           R+YGFEEVD+PVLESEALFIRKAGEEIRDQLYCFEDRGNRRVALRPELTPSLARLVIQKG
Sbjct: 121 RLYGFEEVDYPVLESEALFIRKAGEEIRDQLYCFEDRGNRRVALRPELTPSLARLVIQKG 180

Query: 181 KSLSLPLKWFAIGQCWRYERMTRGRRREHYQWNMDIIGVPEVTAEAELLSSIVTFFKRIG 240
           KSLSLP+KWFAIGQCWRYERMTRGRRREHYQWNMDI+GVPEVTAEAELLSSIVTFFKR+G
Sbjct: 181 KSLSLPIKWFAIGQCWRYERMTRGRRREHYQWNMDILGVPEVTAEAELLSSIVTFFKRVG 240

Query: 241 ITASDVGFKISSRKVLQEVLTCYGIPENLFGKVCIIIDKIEKIPLDEMKKELKVVGVSEE 300
           ITASDVGFK+SSRKVLQ+VL CYGIP+N+FGKVCIIIDKIEKIPLDEMKKELKVVGVSE+
Sbjct: 241 ITASDVGFKVSSRKVLQDVLKCYGIPQNMFGKVCIIIDKIEKIPLDEMKKELKVVGVSED 300

Query: 301 AIEELLQVLSVKSLTKLEEILGGGGEALSDLKQLFSLAEKFGYADWIQFDASIVRGLAYY 360
           AIEELLQVLSVKSLTKLEE+LGGGGEALSDLKQLFSLAEKFGY+DWIQFDASIVRGLAYY
Sbjct: 301 AIEELLQVLSVKSLTKLEEVLGGGGEALSDLKQLFSLAEKFGYSDWIQFDASIVRGLAYY 360

Query: 361 TGIVFEGFDRGGKLRAICGGGRYDRLLSTFGGDDIPACGFGFGDAVIVELLKEKKLLPEL 420
           TGIVFEGFDRGGKLRAICGGGRYDRLLSTFGGDDIPACGFGFGDAVIVELLKEKKLLPEL
Sbjct: 361 TGIVFEGFDRGGKLRAICGGGRYDRLLSTFGGDDIPACGFGFGDAVIVELLKEKKLLPEL 420

Query: 421 NVGIENIVCALDPDLQGAASTVATILREKGQTVDLVLENKPLKWVFKRATRINAQRLILV 480
           N  +ENIVCALDPDLQGAAS VATILREKGQTVDLVLENKPLKWVFKRATRINA+RLILV
Sbjct: 421 NAEVENIVCALDPDLQGAASKVATILREKGQTVDLVLENKPLKWVFKRATRINARRLILV 480

Query: 481 GSSEWQRGVVGVKDLSSGEQREVKLDELN 506
           GSSEW+RG+VGVKDLSSGEQ EVKLDELN
Sbjct: 481 GSSEWERGMVGVKDLSSGEQCEVKLDELN 507

BLAST of Sed0000088 vs. ExPASy TrEMBL
Match: A0A5D3C5Z0 (Histidine--tRNA ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold202G00380 PE=3 SV=1)

HSP 1 Score: 920.2 bits (2377), Expect = 3.7e-264
Identity = 466/509 (91.55%), Postives = 491/509 (96.46%), Query Frame = 0

Query: 1   MAAKPSSLLIFKPRLNLHLHLKPFIFSSSSLAFNIILRFPFPKPSFFPNRKFHSS-RAAS 60
           MAAKPSSLLIF+PRLN  L LKPFIFSSSSLAF+IILRFPFPKPSFF NR F+SS  A S
Sbjct: 1   MAAKPSSLLIFQPRLN--LRLKPFIFSSSSLAFSIILRFPFPKPSFFVNRHFYSSLTAVS 60

Query: 61  SAQSLSGGG---GRSGALAPAPATEEVQKIDVNPPKGTRDFPPEDMRLRNWLFSNFREVS 120
           SAQS+S GG   GRSGAL+PAP TEE+Q+IDVNPPKGTRDFPPEDMRLRNWLF+NFREVS
Sbjct: 61  SAQSISDGGTTRGRSGALSPAPTTEELQRIDVNPPKGTRDFPPEDMRLRNWLFNNFREVS 120

Query: 121 RVYGFEEVDFPVLESEALFIRKAGEEIRDQLYCFEDRGNRRVALRPELTPSLARLVIQKG 180
           R+YGFEEVD+PVLESEALFIRKAGEEIRDQLYCFEDRGNRRVALRPELTPSLARLVIQKG
Sbjct: 121 RLYGFEEVDYPVLESEALFIRKAGEEIRDQLYCFEDRGNRRVALRPELTPSLARLVIQKG 180

Query: 181 KSLSLPLKWFAIGQCWRYERMTRGRRREHYQWNMDIIGVPEVTAEAELLSSIVTFFKRIG 240
           KSLSLP+KWFAIGQCWRYERMTRGRRREHYQWNMDI+GVPEVTAEAELLSSIVTFFKR+G
Sbjct: 181 KSLSLPIKWFAIGQCWRYERMTRGRRREHYQWNMDILGVPEVTAEAELLSSIVTFFKRVG 240

Query: 241 ITASDVGFKISSRKVLQEVLTCYGIPENLFGKVCIIIDKIEKIPLDEMKKELKVVGVSEE 300
           ITASDVGFK+SSRKVLQ+VL CYGIP+N+FGKVCIIIDKIEKIPLDEMKKELKVVGVSE+
Sbjct: 241 ITASDVGFKVSSRKVLQDVLKCYGIPQNMFGKVCIIIDKIEKIPLDEMKKELKVVGVSED 300

Query: 301 AIEELLQVLSVKSLTKLEEILGGGGEALSDLKQLFSLAEKFGYADWIQFDASIVRGLAYY 360
           AIEELLQVLSVKSLTKLEE+LGGGGEALSDLKQLFSLAEKFGY+DWIQFDASIVRGLAYY
Sbjct: 301 AIEELLQVLSVKSLTKLEEVLGGGGEALSDLKQLFSLAEKFGYSDWIQFDASIVRGLAYY 360

Query: 361 TGIVFEGFDRGGKLRAICGGGRYDRLLSTFGGDDIPACGFGFGDAVIVELLKEKKLLPEL 420
           TGIVFEGFDRGGKLRAICGGGRYDRLLSTFGGDDIPACGFGFGDAVIVELLKEKKLLPEL
Sbjct: 361 TGIVFEGFDRGGKLRAICGGGRYDRLLSTFGGDDIPACGFGFGDAVIVELLKEKKLLPEL 420

Query: 421 NVGIENIVCALDPDLQGAASTVATILREKGQTVDLVLENKPLKWVFKRATRINAQRLILV 480
           N  +ENIVCALDPDLQGAAS VAT+LREKGQTVDLVLENKPLKWVFKRATRINA+RLILV
Sbjct: 421 NAEVENIVCALDPDLQGAASKVATVLREKGQTVDLVLENKPLKWVFKRATRINARRLILV 480

Query: 481 GSSEWQRGVVGVKDLSSGEQREVKLDELN 506
           GSSEW+RG+VGVKDLSSGEQ EVKLDELN
Sbjct: 481 GSSEWERGMVGVKDLSSGEQCEVKLDELN 507

BLAST of Sed0000088 vs. ExPASy TrEMBL
Match: A0A6J1EG18 (Histidine--tRNA ligase OS=Cucurbita moschata OX=3662 GN=LOC111433048 PE=3 SV=1)

HSP 1 Score: 918.7 bits (2373), Expect = 1.1e-263
Identity = 470/509 (92.34%), Postives = 486/509 (95.48%), Query Frame = 0

Query: 1   MAAKPSSLLIFKPRLNLHLHLKPFIFSSSSLAFNIILRFPFPKPSFFPNRKFHSS-RAAS 60
           MAAKPSSLLIF+PRLN  L LKPFIFSSSSLAF+IILR PFPK SFF NRKFHSS  A S
Sbjct: 1   MAAKPSSLLIFQPRLN--LRLKPFIFSSSSLAFSIILRIPFPKASFFGNRKFHSSLSAVS 60

Query: 61  SAQSL---SGGGGRSGALAPAPATEEVQKIDVNPPKGTRDFPPEDMRLRNWLFSNFREVS 120
           SAQS      GGGRSGAL+P   TEE+QKIDVNPPKGTRDFPPEDMRLRNWLF+NFREVS
Sbjct: 61  SAQSTGDGGNGGGRSGALSPTLVTEELQKIDVNPPKGTRDFPPEDMRLRNWLFNNFREVS 120

Query: 121 RVYGFEEVDFPVLESEALFIRKAGEEIRDQLYCFEDRGNRRVALRPELTPSLARLVIQKG 180
           RVYGFEEVDFPVLESEALFIRKAGEEIRDQLYCFEDRGNRRVALRPELTPSLARLVIQKG
Sbjct: 121 RVYGFEEVDFPVLESEALFIRKAGEEIRDQLYCFEDRGNRRVALRPELTPSLARLVIQKG 180

Query: 181 KSLSLPLKWFAIGQCWRYERMTRGRRREHYQWNMDIIGVPEVTAEAELLSSIVTFFKRIG 240
           KSLSLPLKWFAIGQCWRYERMTRGRRREHYQWNMDIIGVPEVTAEAELLSSIV FFKRIG
Sbjct: 181 KSLSLPLKWFAIGQCWRYERMTRGRRREHYQWNMDIIGVPEVTAEAELLSSIVAFFKRIG 240

Query: 241 ITASDVGFKISSRKVLQEVLTCYGIPENLFGKVCIIIDKIEKIPLDEMKKELKVVGVSEE 300
           +TA+DVGFK+SSRKVLQEVL CYGIPEN+FGKVCIIIDKIEKIPLDEMKKELKVVGVSE+
Sbjct: 241 LTAADVGFKVSSRKVLQEVLKCYGIPENMFGKVCIIIDKIEKIPLDEMKKELKVVGVSED 300

Query: 301 AIEELLQVLSVKSLTKLEEILGGGGEALSDLKQLFSLAEKFGYADWIQFDASIVRGLAYY 360
           AIEE+LQVLSVKSLTKLEEILGGGGEA+SDLKQLFSLAEKFGY+DWIQFDASIVRGLAYY
Sbjct: 301 AIEEMLQVLSVKSLTKLEEILGGGGEAMSDLKQLFSLAEKFGYSDWIQFDASIVRGLAYY 360

Query: 361 TGIVFEGFDRGGKLRAICGGGRYDRLLSTFGGDDIPACGFGFGDAVIVELLKEKKLLPEL 420
           TGIVFEGFDRGGKLRAICGGGRYDRLLSTFGGDD+PACGFGFGDAVIVELLKEKKLLPEL
Sbjct: 361 TGIVFEGFDRGGKLRAICGGGRYDRLLSTFGGDDLPACGFGFGDAVIVELLKEKKLLPEL 420

Query: 421 NVGIENIVCALDPDLQGAASTVATILREKGQTVDLVLENKPLKWVFKRATRINAQRLILV 480
           NV +ENIVCALDPDLQGAAS VATILR KGQTVDLVLENKPLKWVFKRATRINAQRLILV
Sbjct: 421 NVEVENIVCALDPDLQGAASRVATILRGKGQTVDLVLENKPLKWVFKRATRINAQRLILV 480

Query: 481 GSSEWQRGVVGVKDLSSGEQREVKLDELN 506
           GSSEWQRG+VGVKDLSSGEQREVKLDELN
Sbjct: 481 GSSEWQRGMVGVKDLSSGEQREVKLDELN 507

BLAST of Sed0000088 vs. TAIR 10
Match: AT3G46100.1 (Histidyl-tRNA synthetase 1 )

HSP 1 Score: 744.2 bits (1920), Expect = 6.9e-215
Identity = 371/459 (80.83%), Postives = 415/459 (90.41%), Query Frame = 0

Query: 48  PNRKFHSSRAASSAQSLSGGGGRSGALAPAPATEE--VQKIDVNPPKGTRDFPPEDMRLR 107
           P R+F   R    A   + GGGRSG++     TEE   +KIDVNPPKGTRDFPPEDMRLR
Sbjct: 29  PPRQFSIPRRLICA--AANGGGRSGSIVAPLVTEEDFHKKIDVNPPKGTRDFPPEDMRLR 88

Query: 108 NWLFSNFREVSRVYGFEEVDFPVLESEALFIRKAGEEIRDQLYCFEDRGNRRVALRPELT 167
           NWLF++F+EVSR+YG+EEVD+PVLE+EALFIRKAGEEIRDQLYCFEDRGNRRVALRPELT
Sbjct: 89  NWLFNHFKEVSRLYGYEEVDYPVLETEALFIRKAGEEIRDQLYCFEDRGNRRVALRPELT 148

Query: 168 PSLARLVIQKGKSLSLPLKWFAIGQCWRYERMTRGRRREHYQWNMDIIGVPEVTAEAELL 227
           PSLARLVIQKGKS+SLPLKWFAIGQCWRYERMTRGRRREHYQWNMDIIGVP+VTAEAEL+
Sbjct: 149 PSLARLVIQKGKSVSLPLKWFAIGQCWRYERMTRGRRREHYQWNMDIIGVPQVTAEAELI 208

Query: 228 SSIVTFFKRIGITASDVGFKISSRKVLQEVLTCYGIPENLFGKVCIIIDKIEKIPLDEMK 287
           SSIVTFFKRIGITASDVGFK+SSRKVLQE+L  YG+PE+LFG+VCIIIDKIEKIP+DE+K
Sbjct: 209 SSIVTFFKRIGITASDVGFKVSSRKVLQELLKKYGVPEDLFGRVCIIIDKIEKIPIDEIK 268

Query: 288 KELKVVGVSEEAIEELLQVLSVKSLTKLEEILGGGGEALSDLKQLFSLAEKFGYADWIQF 347
           KEL   G+SE+AIE+LLQVLSVKSL  LE+I+GG GEA++DLKQLFSLAEKFGY++WIQF
Sbjct: 269 KELGFTGISEDAIEQLLQVLSVKSLDDLEDIIGGAGEAIADLKQLFSLAEKFGYSEWIQF 328

Query: 348 DASIVRGLAYYTGIVFEGFDRGGKLRAICGGGRYDRLLSTFGGDDIPACGFGFGDAVIVE 407
           DAS+VRGLAYYTGIVFEGFDR GKLRAICGGGRYDRLLST+GGDD PACGFGFGDAVIVE
Sbjct: 329 DASVVRGLAYYTGIVFEGFDRKGKLRAICGGGRYDRLLSTYGGDDFPACGFGFGDAVIVE 388

Query: 408 LLKEKKLLPELNVGIENIVCALDPDLQGAASTVATILREKGQTVDLVLENKPLKWVFKRA 467
           LLKEK LLPEL   +ENIVCALD DLQGAA+TVAT LR+KGQTVDLVLE+KPLKWVFKRA
Sbjct: 389 LLKEKDLLPELGQEVENIVCALDKDLQGAAATVATALRDKGQTVDLVLESKPLKWVFKRA 448

Query: 468 TRINAQRLILVGSSEWQRGVVGVKDLSSGEQREVKLDEL 505
            R+NA+RL+LVG +EW+ G V VK LSSGEQ +VKL +L
Sbjct: 449 ARVNARRLVLVGKTEWEDGSVSVKVLSSGEQFQVKLSDL 485

BLAST of Sed0000088 vs. TAIR 10
Match: AT5G03406.1 (Class II aaRS and biotin synthetases superfamily protein )

HSP 1 Score: 387.1 bits (993), Expect = 2.1e-107
Identity = 194/256 (75.78%), Postives = 219/256 (85.55%), Query Frame = 0

Query: 28  SSSLAFNIILRFPFPKPSFFPNRKFHSSRAASSAQSLSGGGGRSGAL-APAPATEEVQKI 87
           SSS    ++L F     S  P R+F + R    A S   GGGRSG++ AP    EE Q I
Sbjct: 12  SSSFIRPVLLDF----VSCSPLRQFSTPRRFICAASKVNGGGRSGSIVAPLVVNEEFQNI 71

Query: 88  DVNPPKGTRDFPPEDMRLRNWLFSNFREVSRVYGFEEVDFPVLESEALFIRKAGEEIRDQ 147
           DVNPPKGTRDFPPEDMRLRNWLF++F+EVSR++G+EEVDFPVLE+EALFIRKAGEEIRDQ
Sbjct: 72  DVNPPKGTRDFPPEDMRLRNWLFNHFKEVSRLFGYEEVDFPVLETEALFIRKAGEEIRDQ 131

Query: 148 LYCFEDRGNRRVALRPELTPSLARLVIQKGKSLSLPLKWFAIGQCWRYERMTRGRRREHY 207
           LYCFEDRGNRRVALRPELTPSLARLVIQKGKS+SLPLKWFA+GQCWRYERMTRGRRREHY
Sbjct: 132 LYCFEDRGNRRVALRPELTPSLARLVIQKGKSVSLPLKWFAVGQCWRYERMTRGRRREHY 191

Query: 208 QWNMDIIGVPEVTAEAELLSSIVTFFKRIGITASDVGFKISSRKVLQEVLTCYGIPENLF 267
           QWNMDIIGVPEVTAEAEL+SSI+TFFKRIGITASDVGFK+SSRKVLQE L  YG+PE+LF
Sbjct: 192 QWNMDIIGVPEVTAEAELISSIMTFFKRIGITASDVGFKVSSRKVLQEFLKKYGVPEDLF 251

Query: 268 GKVCIIIDKIEKIPLD 283
           G+VC IIDK+  + ++
Sbjct: 252 GRVCFIIDKVSNLNIN 263

BLAST of Sed0000088 vs. TAIR 10
Match: AT3G02760.1 (Class II aaRS and biotin synthetases superfamily protein )

HSP 1 Score: 163.7 bits (413), Expect = 3.9e-40
Identity = 126/426 (29.58%), Postives = 204/426 (47.89%), Query Frame = 0

Query: 91  PKGTRDFPPEDMRLRNWLFSNFREVSRVYGFEEVDFPVLESEALFIRKAGEEIRDQLYCF 150
           PKGTRDF  E M +R   FS  + V + +G   +D PV E     + K GE+ +  +Y  
Sbjct: 449 PKGTRDFAKEQMAVREKAFSIIQNVFKRHGATALDTPVFELRETLMGKYGEDSK-LVYDI 508

Query: 151 EDRGNRRVALRPELTPSLARLVIQKGKSLSLPLKWFAIGQCWRYERMTRGRRREHYQWNM 210
            D+G    +LR +LT   AR V   G +     K + I + +R +  ++GR RE YQ + 
Sbjct: 509 ADQGGELCSLRYDLTVPFARYVAMNGIT---SFKRYQIAKVYRRDNPSKGRYREFYQCDF 568

Query: 211 DIIGVPE-VTAEAELLSSIVTFFKRIGITASDVGFKISSRKVLQEVLTCYGIPENLFGKV 270
           DI G+ E +  + E++  +      + I   D   K++ RK+L  +L   G+P   F  +
Sbjct: 569 DIAGLFEPMGPDFEIVKILTELLDELEI--GDYEVKLNHRKLLDGMLEICGVPPEKFRTI 628

Query: 271 CIIIDKIEKIPLDEMKKEL-KVVGVSEEAIEELLQVLSVKS-----LTKL----EEILG- 330
           C  IDK++K   +++KKE+ +  G+S E  + +   +  K      L+KL     E L  
Sbjct: 629 CSSIDKLDKQSFEQVKKEMVEEKGLSSEIADRIGNFVKEKGAPLELLSKLRQEGSEFLDN 688

Query: 331 -GGGEALSDLKQLFSLAEKFGYADWIQFDASIVRGLAYYTGIVFEGFDRGGKLRAICGGG 390
               EAL +L  +F   ++   ++ I FD S+ RGL YYTG++FE    G ++ +I  GG
Sbjct: 689 QSSREALDELSIMFEALKRSKCSERIVFDLSLARGLDYYTGVIFEAVCIGAEVGSIGAGG 748

Query: 391 RYDRLLSTFGGDDIPACGFGFGDAVIVELL-----KEKKLLPELNVGIENIVCALDPDLQ 450
           RYD L+  FG   +PA G   G   +  ++     K+K+++      +  +   +D  L 
Sbjct: 749 RYDNLIGMFGTKQVPAVGMSLGIERVFNIMEELNEKQKQVIRPTETQV-LVSIMVDNKLA 808

Query: 451 GAASTVATILREKGQTVDLVLENKPLKWVFKRATRINAQRLILVGSSEWQRGVVGVKDLS 499
            AA  V+ +   K     LV + K     F RA       +++VG  E     V +K L 
Sbjct: 809 EAAELVSQLWGAKINAEYLVSKRKEKH--FNRAKESGIPWMVMVGEKELSGSFVTLKKLE 865

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038883346.15.2e-26591.55histidine--tRNA ligase, chloroplastic/mitochondrial [Benincasa hispida][more]
XP_022978407.14.4e-26492.14histidine--tRNA ligase, chloroplastic/mitochondrial isoform X2 [Cucurbita maxima... [more]
XP_008462490.15.8e-26491.75PREDICTED: histidine--tRNA ligase, chloroplastic/mitochondrial [Cucumis melo] >K... [more]
TYK07323.17.6e-26491.55histidine--tRNA ligase [Cucumis melo var. makuwa][more]
XP_023544233.11.3e-26392.34histidine--tRNA ligase, chloroplastic/mitochondrial isoform X2 [Cucurbita pepo s... [more]
Match NameE-valueIdentityDescription
O824139.7e-21480.83Histidine--tRNA ligase, chloroplastic/mitochondrial OS=Arabidopsis thaliana OX=3... [more]
Q8U4311.6e-7036.17Histidine--tRNA ligase OS=Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JC... [more]
Q9HLX52.7e-7035.46Histidine--tRNA ligase OS=Thermoplasma acidophilum (strain ATCC 25905 / DSM 1728... [more]
O580281.3e-6937.53Histidine--tRNA ligase OS=Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 ... [more]
Q9UY311.7e-6937.35Histidine--tRNA ligase OS=Pyrococcus abyssi (strain GE5 / Orsay) OX=272844 GN=hi... [more]
Match NameE-valueIdentityDescription
A0A6J1IL102.1e-26492.14Histidine--tRNA ligase OS=Cucurbita maxima OX=3661 GN=LOC111478405 PE=3 SV=1[more]
A0A5A7VDI22.8e-26491.75Histidine--tRNA ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold8... [more]
A0A1S3CHK22.8e-26491.75Histidine--tRNA ligase OS=Cucumis melo OX=3656 GN=LOC103500830 PE=3 SV=1[more]
A0A5D3C5Z03.7e-26491.55Histidine--tRNA ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold2... [more]
A0A6J1EG181.1e-26392.34Histidine--tRNA ligase OS=Cucurbita moschata OX=3662 GN=LOC111433048 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT3G46100.16.9e-21580.83Histidyl-tRNA synthetase 1 [more]
AT5G03406.12.1e-10775.78Class II aaRS and biotin synthetases superfamily protein [more]
AT3G02760.13.9e-4029.58Class II aaRS and biotin synthetases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Chayote (edule) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004154Anticodon-bindingPFAMPF03129HGTP_anticodoncoord: 426..504
e-value: 2.3E-9
score: 37.2
IPR036621Anticodon-binding domain superfamilyGENE3D3.40.50.800coord: 413..505
e-value: 3.3E-18
score: 67.1
NoneNo IPR availableGENE3D3.30.930.10Bira Bifunctional Protein; Domain 2coord: 84..412
e-value: 2.1E-116
score: 390.4
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 58..97
NoneNo IPR availablePANTHERPTHR43707:SF1HISTIDINE--TRNA LIGASE, MITOCHONDRIAL-RELATEDcoord: 84..504
NoneNo IPR availableSUPERFAMILY55681Class II aaRS and biotin synthetasescoord: 90..409
NoneNo IPR availableSUPERFAMILY52954Class II aaRS ABD-relatedcoord: 422..504
IPR004516Histidine-tRNA ligase/ATP phosphoribosyltransferase regulatory subunitPIRSFPIRSF001549His-tRNA_synthcoord: 84..505
e-value: 2.5E-146
score: 485.7
IPR004516Histidine-tRNA ligase/ATP phosphoribosyltransferase regulatory subunitPANTHERPTHR43707HISTIDYL-TRNA SYNTHETASEcoord: 84..504
IPR041715Class II Histidinyl-tRNA synthetase (HisRS)-like catalytic core domainPFAMPF13393tRNA-synt_Hiscoord: 93..397
e-value: 3.5E-47
score: 161.2
IPR041715Class II Histidinyl-tRNA synthetase (HisRS)-like catalytic core domainCDDcd00773HisRS-like_corecoord: 101..408
e-value: 5.66514E-98
score: 294.51
IPR015807Histidine-tRNA ligaseTIGRFAMTIGR00442TIGR00442coord: 90..500
e-value: 3.0E-104
score: 347.2
IPR015807Histidine-tRNA ligaseHAMAPMF_00127His_tRNA_synthcoord: 86..505
score: 25.158855
IPR006195Aminoacyl-tRNA synthetase, class IIPROSITEPS50862AA_TRNA_LIGASE_IIcoord: 93..414
score: 13.693316

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sed0000088.1Sed0000088.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006427 histidyl-tRNA aminoacylation
cellular_component GO:0005737 cytoplasm
molecular_function GO:0005524 ATP binding
molecular_function GO:0004821 histidine-tRNA ligase activity