Pay0013278 (gene) Melon (Payzawat) v1

Overview
NamePay0013278
Typegene
OrganismCucumis melo L. var. inodorus cv. Payzawat (Melon (Payzawat) v1)
DescriptionMitogen-activated protein kinase kinase kinase 3-like
LocationContig00140_ERROPOS1480835: 47840 .. 48904 (-)
RNA-Seq ExpressionPay0013278
SyntenyPay0013278
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATTGGGTTCGAGGACATGAGATTGGACATGGAAGTTTCGCTACCATCAATTTGGCCACGCCTTCTTCAGGTGATCGATTTCCTCCCTTAATGGCCGTTAAATCCTCTGAACTTGTTTGCTCTTCCTCGCTCAAGAACGAGAAGCAAATTTTGGATCTTCTTGGTGATGATTGCCCACAAATTATTCGGTGTTTCGGGGAGAGTTGTAGTGTCGAGAACGGCGAGGAGCTTTACAATCTGTTTCTCGAGTATGCTACTGGTGGGAGTTTAGCCGATAGAATACAGAGCTGCGGTGGTCGGCTGCCGGAATTTGAGGTTCGACGGTACACGAGGACTATTCTTGAAGGGCTTCGGTATATTCATGGAAAGGGGTTTGTTCACTGCGATGTTAAGCCTAGGAATATTCTCGTGTTTGGTGACGGCGATGCAAAGATTGCCGATTTCGGATTGTCGAAGAAGGCGGGAAAGAACAGGGTAGAAACAGGGGAAGAGACAGGGAGATTTCAATTGAGAGGCTCTCCTCTGTACATGTCGCCGGAATCGCTTAACGATAATGAGTATGAGTCGCCGTGTGACATATGGGCCGTCGGATGCGCGGTGGTGGAGATGTTCACAGGGAAGCCCGCGTGGAATTGTCGGCCGGAATCCAACGTGTTTGCTTTGTTAATCAAAATTGGGATTGGAGAAGAATTGCCGGAAATCCCTAAAGATCTATCGGAAGAAGGGAAAGACTTTCTCAGAAAATGTTTGGTGAAGGATCCAGTAAAGAGATGGACGGCCGATATGCTTCTAAAACACCCGTTCGTAGCAGACTCCGGGAGGTGTGTTCCATTGGGGGAAATGGACGAAGTATCGACGTCGCCAAGATGTCCTTTCGATTTCGAAGATTGGGCATCGATTCATTCACAAGAGAGGGATCCTAGAAATGAAGAGGAATCAAATTGTTGGTTGAATTCGTCGTGTTCGTGGTCGTCGTCTTCTCCAAGGGAGAGGCTGCTTCAACTAGTGGAGAAAGGGGCAGTTGACTGGTCGGTCACTGACAATTGGGTCAGGGTCAGATGA

mRNA sequence

ATGGATTGGGTTCGAGGACATGAGATTGGACATGGAAGTTTCGCTACCATCAATTTGGCCACGCCTTCTTCAGGTGATCGATTTCCTCCCTTAATGGCCGTTAAATCCTCTGAACTTGTTTGCTCTTCCTCGCTCAAGAACGAGAAGCAAATTTTGGATCTTCTTGGTGATGATTGCCCACAAATTATTCGGTGTTTCGGGGAGAGTTGTAGTGTCGAGAACGGCGAGGAGCTTTACAATCTGTTTCTCGAGTATGCTACTGGTGGGAGTTTAGCCGATAGAATACAGAGCTGCGGTGGTCGGCTGCCGGAATTTGAGGTTCGACGGTACACGAGGACTATTCTTGAAGGGCTTCGGTATATTCATGGAAAGGGGTTTGTTCACTGCGATGTTAAGCCTAGGAATATTCTCGTGTTTGGTGACGGCGATGCAAAGATTGCCGATTTCGGATTGTCGAAGAAGGCGGGAAAGAACAGGGTAGAAACAGGGGAAGAGACAGGGAGATTTCAATTGAGAGGCTCTCCTCTGTACATGTCGCCGGAATCGCTTAACGATAATGAGTATGAGTCGCCGTGTGACATATGGGCCGTCGGATGCGCGGTGGTGGAGATGTTCACAGGGAAGCCCGCGTGGAATTGTCGGCCGGAATCCAACGTGTTTGCTTTGTTAATCAAAATTGGGATTGGAGAAGAATTGCCGGAAATCCCTAAAGATCTATCGGAAGAAGGGAAAGACTTTCTCAGAAAATGTTTGGTGAAGGATCCAGTAAAGAGATGGACGGCCGATATGCTTCTAAAACACCCGTTCGTAGCAGACTCCGGGAGGTGTGTTCCATTGGGGGAAATGGACGAAGTATCGACGTCGCCAAGATGTCCTTTCGATTTCGAAGATTGGGCATCGATTCATTCACAAGAGAGGGATCCTAGAAATGAAGAGGAATCAAATTGTTGGTTGAATTCGTCGTGTTCGTGGTCGTCGTCTTCTCCAAGGGAGAGGCTGCTTCAACTAGTGGAGAAAGGGGCAGTTGACTGGTCGGTCACTGACAATTGGGTCAGGGTCAGATGA

Coding sequence (CDS)

ATGGATTGGGTTCGAGGACATGAGATTGGACATGGAAGTTTCGCTACCATCAATTTGGCCACGCCTTCTTCAGGTGATCGATTTCCTCCCTTAATGGCCGTTAAATCCTCTGAACTTGTTTGCTCTTCCTCGCTCAAGAACGAGAAGCAAATTTTGGATCTTCTTGGTGATGATTGCCCACAAATTATTCGGTGTTTCGGGGAGAGTTGTAGTGTCGAGAACGGCGAGGAGCTTTACAATCTGTTTCTCGAGTATGCTACTGGTGGGAGTTTAGCCGATAGAATACAGAGCTGCGGTGGTCGGCTGCCGGAATTTGAGGTTCGACGGTACACGAGGACTATTCTTGAAGGGCTTCGGTATATTCATGGAAAGGGGTTTGTTCACTGCGATGTTAAGCCTAGGAATATTCTCGTGTTTGGTGACGGCGATGCAAAGATTGCCGATTTCGGATTGTCGAAGAAGGCGGGAAAGAACAGGGTAGAAACAGGGGAAGAGACAGGGAGATTTCAATTGAGAGGCTCTCCTCTGTACATGTCGCCGGAATCGCTTAACGATAATGAGTATGAGTCGCCGTGTGACATATGGGCCGTCGGATGCGCGGTGGTGGAGATGTTCACAGGGAAGCCCGCGTGGAATTGTCGGCCGGAATCCAACGTGTTTGCTTTGTTAATCAAAATTGGGATTGGAGAAGAATTGCCGGAAATCCCTAAAGATCTATCGGAAGAAGGGAAAGACTTTCTCAGAAAATGTTTGGTGAAGGATCCAGTAAAGAGATGGACGGCCGATATGCTTCTAAAACACCCGTTCGTAGCAGACTCCGGGAGGTGTGTTCCATTGGGGGAAATGGACGAAGTATCGACGTCGCCAAGATGTCCTTTCGATTTCGAAGATTGGGCATCGATTCATTCACAAGAGAGGGATCCTAGAAATGAAGAGGAATCAAATTGTTGGTTGAATTCGTCGTGTTCGTGGTCGTCGTCTTCTCCAAGGGAGAGGCTGCTTCAACTAGTGGAGAAAGGGGCAGTTGACTGGTCGGTCACTGACAATTGGGTCAGGGTCAGATGA

Protein sequence

MDWVRGHEIGHGSFATINLATPSSGDRFPPLMAVKSSELVCSSSLKNEKQILDLLGDDCPQIIRCFGESCSVENGEELYNLFLEYATGGSLADRIQSCGGRLPEFEVRRYTRTILEGLRYIHGKGFVHCDVKPRNILVFGDGDAKIADFGLSKKAGKNRVETGEETGRFQLRGSPLYMSPESLNDNEYESPCDIWAVGCAVVEMFTGKPAWNCRPESNVFALLIKIGIGEELPEIPKDLSEEGKDFLRKCLVKDPVKRWTADMLLKHPFVADSGRCVPLGEMDEVSTSPRCPFDFEDWASIHSQERDPRNEEESNCWLNSSCSWSSSSPRERLLQLVEKGAVDWSVTDNWVRVR
Homology
BLAST of Pay0013278 vs. ExPASy Swiss-Prot
Match: Q9SND6 (Mitogen-activated protein kinase kinase kinase 20 OS=Arabidopsis thaliana OX=3702 GN=MAPKKK20 PE=1 SV=1)

HSP 1 Score: 367.5 bits (942), Expect = 1.7e-100
Identity = 189/364 (51.92%), Postives = 247/364 (67.86%), Query Frame = 0

Query: 1   MDWVRGHEIGHGSFATINLATPS--SGDRFPPLMAVKSSELVCSSSLKNEKQILDLLGDD 60
           M+WVRG  IG G+F+T++ AT S  SGD FP L+AVKS++   ++SL NEK +LD LG D
Sbjct: 1   MEWVRGETIGFGTFSTVSTATKSRNSGD-FPALIAVKSTDAYGAASLSNEKSVLDSLG-D 60

Query: 61  CPQIIRCFGESCSVENGEELYNLFLEYATGGSLADRIQSCGGR-LPEFEVRRYTRTILEG 120
           CP+IIRC+GE  +VENGEE++NL LEYA+ GSLA  ++  GG  LPE  VRR+T ++L G
Sbjct: 61  CPEIIRCYGEDSTVENGEEMHNLLLEYASRGSLASYMKKLGGEGLPESTVRRHTGSVLRG 120

Query: 121 LRYIHGKGFVHCDVKPRNILVFGDGDAKIADFGLSKKAGKNRVETGEETGRFQLRGSPLY 180
           LR+IH KGF HCD+K  NIL+F DG  KIADFGL+ +   +     +     ++RG+PLY
Sbjct: 121 LRHIHAKGFAHCDIKLANILLFNDGSVKIADFGLAMRVDGDLTALRKSV---EIRGTPLY 180

Query: 181 MSPESLNDNEYESPCDIWAVGCAVVEMFTGKPAWNCRPESNVFALLIKIGIGEELPEIPK 240
           M+PE +NDNEY S  D+WA+GCAVVEMF+GK AW+ +  S+  +LLI+IG+G+ELP+IP+
Sbjct: 181 MAPECVNDNEYGSAADVWALGCAVVEMFSGKTAWSVKEGSHFMSLLIRIGVGDELPKIPE 240

Query: 241 DLSEEGKDFLRKCLVKDPVKRWTADMLLKHPFVA----DSGR---CVPLGEMDEVSTSPR 300
            LSEEGKDFL KC VKDP KRWTA+MLL H FV     D  R    V + + D+V  SP+
Sbjct: 241 MLSEEGKDFLSKCFVKDPAKRWTAEMLLNHSFVTIDLEDDHRENFVVKVKDEDKVLMSPK 300

Query: 301 CPFDFEDWASIHSQERDPRNEEESNCWLNSSCSWSSSSPRERLLQLVEKGAVDWSVTDNW 355
           CPF+F+DW S  + + +P                S  SP ERL  LV     DWSV  +W
Sbjct: 301 CPFEFDDWDSF-TLDSNP----------------SFDSPVERLGSLVSGSIPDWSVGGSW 342

BLAST of Pay0013278 vs. ExPASy Swiss-Prot
Match: O80888 (Mitogen-activated protein kinase kinase kinase 17 OS=Arabidopsis thaliana OX=3702 GN=MAPKKK17 PE=1 SV=1)

HSP 1 Score: 231.9 bits (590), Expect = 1.1e-59
Identity = 138/361 (38.23%), Postives = 194/361 (53.74%), Query Frame = 0

Query: 1   MDWVRGHEIGHGSFATINLATPSSGDRFPPLMAVKSSELVCSSSLKNEKQILDLLGDDCP 60
           M+W RG  +G GS AT+  A   + D    ++AVKSSE+  S  L+ E +IL  L    P
Sbjct: 1   MEWTRGRILGRGSTATVYAAAGHNSDE---ILAVKSSEVHRSEFLQREAKILSSLSS--P 60

Query: 61  QIIRCFGESCSVE-NGEELYNLFLEYATGGSLADRIQSCGGRLPEFEVRRYTRTILEGLR 120
            +I   G     E NG  +YNL +EYA  G+L D     GGR+ E  V +YTR IL+GL 
Sbjct: 61  YVIGYRGSETKRESNGVVMYNLLMEYAPYGTLTDAAAKDGGRVDETRVVKYTRDILKGLE 120

Query: 121 YIHGKGFVHCDVKPRNILVFGDGDAKIADFGLSKKAGKNRVETGEETGRFQLRGSPLYMS 180
           YIH KG VHCDVK  N+++   G+AKIADFG +K     RV+   E+    + G+P +M+
Sbjct: 121 YIHSKGIVHCDVKGSNVVISEKGEAKIADFGCAK-----RVDPVFES---PVMGTPAFMA 180

Query: 181 PESLNDNEYESPCDIWAVGCAVVEMFTGKPAW-NCRPESNVFALLIKIGIGEELPEIPKD 240
           PE     +     DIWAVGC ++EM TG P W       +  ++L ++G   E PE+P  
Sbjct: 181 PEVARGEKQGKESDIWAVGCTMIEMVTGSPPWTKADSREDPVSVLYRVGYSSETPELPCL 240

Query: 241 LSEEGKDFLRKCLVKDPVKRWTADMLLKHPFVADSGRCVPLGEMDEVSTSPRCPFDFEDW 300
           L+EE KDFL KCL ++  +RWTA  LL HPF+       P+     +S SP    D   W
Sbjct: 241 LAEEAKDFLEKCLKREANERWTATQLLNHPFLTTKPDIEPVLVPGLISNSPTSVTDQTFW 300

Query: 301 ASIHSQERDPRNE-EESNCWLNSSCSWSSSSPRERLLQLVEKGAVDWSVTD----NWVRV 355
            S+  +E +   E ++ +  L+    W   S  ER+ +L   G +D +  D    +W+ V
Sbjct: 301 RSVEEEEEEETEEIQKDSRDLDRLSLWGCYS--ERIGRLKCVGGLDGTRCDMEGGDWIMV 346

BLAST of Pay0013278 vs. ExPASy Swiss-Prot
Match: Q9ZVP5 (Mitogen-activated protein kinase kinase kinase 18 OS=Arabidopsis thaliana OX=3702 GN=MAPKKK18 PE=1 SV=1)

HSP 1 Score: 208.4 bits (529), Expect = 1.3e-52
Identity = 136/366 (37.16%), Postives = 188/366 (51.37%), Query Frame = 0

Query: 1   MDWVRGHEIGHGSFATINLAT-PSSGDRFPPLMAVKSSELVCSSSLKNEKQILDLLGDDC 60
           M+W RG  +G GS AT++ AT   SG+     +AVKS+E   S  L+ E +IL  L  + 
Sbjct: 1   MNWTRGKTLGRGSTATVSAATCHESGE----TLAVKSAEFHRSEFLQREAKILSSL--NS 60

Query: 61  PQIIRCFGESCSVE----NGE-ELYNLFLEYATGGSLADRIQSCGGRLPEFEVRRYTRTI 120
           P +I   G   + E    NGE   Y+L +EYA  G+L D     GG + E  V +YTR I
Sbjct: 61  PYVIGYRGCEITREPFHNNGEATTYSLLMEYAPYGTLTDVATKNGGFIDEARVVKYTRQI 120

Query: 121 LEGLRYIH-GKGFVHCDVKPRNILVFGDGDAKIADFGLSKKAGKNRVETGEETGRFQLRG 180
           L GL YIH  KG  HCD+K  N+LV  +G+AKIADFG +K       E         +RG
Sbjct: 121 LLGLEYIHNSKGIAHCDIKGSNVLVGENGEAKIADFGCAKWVEPEITE--------PVRG 180

Query: 181 SPLYMSPESLNDNEYESPCDIWAVGCAVVEMFTGKPAWNCRPESNVFALLIKIGIGEELP 240
           +P +M+PE+          DIWAVGC V+EM TG   W     ++  ++L ++G   ELP
Sbjct: 181 TPAFMAPEAARGERQGKESDIWAVGCTVIEMVTGSQPWIGADFTDPVSVLYRVGYLGELP 240

Query: 241 EIPKDLSEEGKDFLRKCLVKDPVKRWTADMLLKHPFVADSGRCVPLGEMDEVSTSPRCPF 300
           E+P  L+E+ KDFL KCL K+  +RWTA  LL HPF+ +    +  G    V+ SP    
Sbjct: 241 ELPCSLTEQAKDFLGKCLKKEATERWTASQLLNHPFLVNKEPELVTG---LVTNSPTSVT 300

Query: 301 DFEDWASIHSQERDPRNEEESNCWLNSSCSWSSSSPRERLLQLVEKGAV----DWSVT-D 355
           D   W S+  +  + R+            SW      ER+  L   G V     W +  +
Sbjct: 301 DQMFWRSVEEEVSEDRS------------SWWECHEDERIGVLSWIGHVVVESTWDLDGE 337

BLAST of Pay0013278 vs. ExPASy Swiss-Prot
Match: F4HRJ4 (Mitogen-activated protein kinase kinase kinase 3 OS=Arabidopsis thaliana OX=3702 GN=MAPKKK3 PE=1 SV=1)

HSP 1 Score: 180.3 bits (456), Expect = 3.9e-44
Identity = 101/277 (36.46%), Postives = 153/277 (55.23%), Query Frame = 0

Query: 3   WVRGHEIGHGSFATINLATPSSGDRFPPLMAVK--SSELVCSSSLKNEKQILDLLGDDC- 62
           W +G  +G G+F  + L   S   +   +  VK  S +      LK   Q ++LL   C 
Sbjct: 214 WKKGKFLGSGTFGQVYLGFNSEKGKMCAIKEVKVISDDQTSKECLKQLNQEINLLNQLCH 273

Query: 63  PQIIRCFGESCSVENGEELYNLFLEYATGGSLADRIQSCGGRLPEFEVRRYTRTILEGLR 122
           P I++ +G   S    EE  +++LEY +GGS+   ++  G    E  ++ YTR IL GL 
Sbjct: 274 PNIVQYYGSELS----EETLSVYLEYVSGGSIHKLLKDYGS-FTEPVIQNYTRQILAGLA 333

Query: 123 YIHGKGFVHCDVKPRNILVFGDGDAKIADFGLSKKAGKNRVETGEETGRFQLRGSPLYMS 182
           Y+HG+  VH D+K  NILV  +G+ K+ADFG++K        T   T     +GSP +M+
Sbjct: 334 YLHGRNTVHRDIKGANILVDPNGEIKLADFGMAKHV------TAFST-MLSFKGSPYWMA 393

Query: 183 PE-SLNDNEYESPCDIWAVGCAVVEMFTGKPAWNCRPESNVFALLIKIGIGEELPEIPKD 242
           PE  ++ N Y    DIW++GC ++EM T KP W+   +    A + KIG  ++ PEIP  
Sbjct: 394 PEVVMSQNGYTHAVDIWSLGCTILEMATSKPPWS---QFEGVAAIFKIGNSKDTPEIPDH 453

Query: 243 LSEEGKDFLRKCLVKDPVKRWTADMLLKHPFVADSGR 276
           LS + K+F+R CL ++P  R TA  LL+HPF+ ++ R
Sbjct: 454 LSNDAKNFIRLCLQRNPTVRPTASQLLEHPFLRNTTR 475

BLAST of Pay0013278 vs. ExPASy Swiss-Prot
Match: O22042 (Mitogen-activated protein kinase kinase kinase 3 OS=Arabidopsis thaliana OX=3702 GN=ANP3 PE=1 SV=1)

HSP 1 Score: 175.3 bits (443), Expect = 1.3e-42
Identity = 103/278 (37.05%), Postives = 151/278 (54.32%), Query Frame = 0

Query: 3   WVRGHEIGHGSFATINLA-TPSSGDRFPPLMAVKSSELVCSSSLKN---------EKQIL 62
           W +G  IG G+F  + +     SG+    L+A+K   +  SS+ K          E+++ 
Sbjct: 68  WRKGELIGCGAFGRVYMGMNLDSGE----LLAIKQVLIAPSSASKEKTQGHIRELEEEVQ 127

Query: 63  DLLGDDCPQIIRCFGESCSVENGEELYNLFLEYATGGSLADRIQSCGGRLPEFEVRRYTR 122
            L     P I+R  G   +V   + L N+ +E+  GGS++  ++  G   PE  +  YT+
Sbjct: 128 LLKNLSHPNIVRYLG---TVRESDSL-NILMEFVPGGSISSLLEKFGS-FPEPVIIMYTK 187

Query: 123 TILEGLRYIHGKGFVHCDVKPRNILVFGDGDAKIADFGLSKKAGKNRVETGEETGRFQLR 182
            +L GL Y+H  G +H D+K  NILV   G  ++ADFG SKK     VE     G   ++
Sbjct: 188 QLLLGLEYLHNNGIMHRDIKGANILVDNKGCIRLADFGASKKV----VELATVNGAKSMK 247

Query: 183 GSPLYMSPESLNDNEYESPCDIWAVGCAVVEMFTGKPAWNCRPESNVFALLIKIGIGEEL 242
           G+P +M+PE +    +    DIW+VGC V+EM TGKP W+   +   FA ++ IG  +  
Sbjct: 248 GTPYWMAPEVILQTGHSFSADIWSVGCTVIEMATGKPPWS--EQYQQFAAVLHIGRTKAH 307

Query: 243 PEIPKDLSEEGKDFLRKCLVKDPVKRWTADMLLKHPFV 271
           P IP+DLS E KDFL KCL K+P  R +A  LL+HPFV
Sbjct: 308 PPIPEDLSPEAKDFLMKCLHKEPSLRLSATELLQHPFV 330

BLAST of Pay0013278 vs. ExPASy TrEMBL
Match: A0A5D3CM36 (Mitogen-activated protein kinase kinase kinase 3-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G004750 PE=3 SV=1)

HSP 1 Score: 751.9 bits (1940), Expect = 1.2e-213
Identity = 354/354 (100.00%), Postives = 354/354 (100.00%), Query Frame = 0

Query: 1   MDWVRGHEIGHGSFATINLATPSSGDRFPPLMAVKSSELVCSSSLKNEKQILDLLGDDCP 60
           MDWVRGHEIGHGSFATINLATPSSGDRFPPLMAVKSSELVCSSSLKNEKQILDLLGDDCP
Sbjct: 1   MDWVRGHEIGHGSFATINLATPSSGDRFPPLMAVKSSELVCSSSLKNEKQILDLLGDDCP 60

Query: 61  QIIRCFGESCSVENGEELYNLFLEYATGGSLADRIQSCGGRLPEFEVRRYTRTILEGLRY 120
           QIIRCFGESCSVENGEELYNLFLEYATGGSLADRIQSCGGRLPEFEVRRYTRTILEGLRY
Sbjct: 61  QIIRCFGESCSVENGEELYNLFLEYATGGSLADRIQSCGGRLPEFEVRRYTRTILEGLRY 120

Query: 121 IHGKGFVHCDVKPRNILVFGDGDAKIADFGLSKKAGKNRVETGEETGRFQLRGSPLYMSP 180
           IHGKGFVHCDVKPRNILVFGDGDAKIADFGLSKKAGKNRVETGEETGRFQLRGSPLYMSP
Sbjct: 121 IHGKGFVHCDVKPRNILVFGDGDAKIADFGLSKKAGKNRVETGEETGRFQLRGSPLYMSP 180

Query: 181 ESLNDNEYESPCDIWAVGCAVVEMFTGKPAWNCRPESNVFALLIKIGIGEELPEIPKDLS 240
           ESLNDNEYESPCDIWAVGCAVVEMFTGKPAWNCRPESNVFALLIKIGIGEELPEIPKDLS
Sbjct: 181 ESLNDNEYESPCDIWAVGCAVVEMFTGKPAWNCRPESNVFALLIKIGIGEELPEIPKDLS 240

Query: 241 EEGKDFLRKCLVKDPVKRWTADMLLKHPFVADSGRCVPLGEMDEVSTSPRCPFDFEDWAS 300
           EEGKDFLRKCLVKDPVKRWTADMLLKHPFVADSGRCVPLGEMDEVSTSPRCPFDFEDWAS
Sbjct: 241 EEGKDFLRKCLVKDPVKRWTADMLLKHPFVADSGRCVPLGEMDEVSTSPRCPFDFEDWAS 300

Query: 301 IHSQERDPRNEEESNCWLNSSCSWSSSSPRERLLQLVEKGAVDWSVTDNWVRVR 355
           IHSQERDPRNEEESNCWLNSSCSWSSSSPRERLLQLVEKGAVDWSVTDNWVRVR
Sbjct: 301 IHSQERDPRNEEESNCWLNSSCSWSSSSPRERLLQLVEKGAVDWSVTDNWVRVR 354

BLAST of Pay0013278 vs. ExPASy TrEMBL
Match: A0A1S3B1I0 (mitogen-activated protein kinase kinase kinase 3-like OS=Cucumis melo OX=3656 GN=LOC103484781 PE=3 SV=1)

HSP 1 Score: 751.9 bits (1940), Expect = 1.2e-213
Identity = 354/354 (100.00%), Postives = 354/354 (100.00%), Query Frame = 0

Query: 1   MDWVRGHEIGHGSFATINLATPSSGDRFPPLMAVKSSELVCSSSLKNEKQILDLLGDDCP 60
           MDWVRGHEIGHGSFATINLATPSSGDRFPPLMAVKSSELVCSSSLKNEKQILDLLGDDCP
Sbjct: 1   MDWVRGHEIGHGSFATINLATPSSGDRFPPLMAVKSSELVCSSSLKNEKQILDLLGDDCP 60

Query: 61  QIIRCFGESCSVENGEELYNLFLEYATGGSLADRIQSCGGRLPEFEVRRYTRTILEGLRY 120
           QIIRCFGESCSVENGEELYNLFLEYATGGSLADRIQSCGGRLPEFEVRRYTRTILEGLRY
Sbjct: 61  QIIRCFGESCSVENGEELYNLFLEYATGGSLADRIQSCGGRLPEFEVRRYTRTILEGLRY 120

Query: 121 IHGKGFVHCDVKPRNILVFGDGDAKIADFGLSKKAGKNRVETGEETGRFQLRGSPLYMSP 180
           IHGKGFVHCDVKPRNILVFGDGDAKIADFGLSKKAGKNRVETGEETGRFQLRGSPLYMSP
Sbjct: 121 IHGKGFVHCDVKPRNILVFGDGDAKIADFGLSKKAGKNRVETGEETGRFQLRGSPLYMSP 180

Query: 181 ESLNDNEYESPCDIWAVGCAVVEMFTGKPAWNCRPESNVFALLIKIGIGEELPEIPKDLS 240
           ESLNDNEYESPCDIWAVGCAVVEMFTGKPAWNCRPESNVFALLIKIGIGEELPEIPKDLS
Sbjct: 181 ESLNDNEYESPCDIWAVGCAVVEMFTGKPAWNCRPESNVFALLIKIGIGEELPEIPKDLS 240

Query: 241 EEGKDFLRKCLVKDPVKRWTADMLLKHPFVADSGRCVPLGEMDEVSTSPRCPFDFEDWAS 300
           EEGKDFLRKCLVKDPVKRWTADMLLKHPFVADSGRCVPLGEMDEVSTSPRCPFDFEDWAS
Sbjct: 241 EEGKDFLRKCLVKDPVKRWTADMLLKHPFVADSGRCVPLGEMDEVSTSPRCPFDFEDWAS 300

Query: 301 IHSQERDPRNEEESNCWLNSSCSWSSSSPRERLLQLVEKGAVDWSVTDNWVRVR 355
           IHSQERDPRNEEESNCWLNSSCSWSSSSPRERLLQLVEKGAVDWSVTDNWVRVR
Sbjct: 301 IHSQERDPRNEEESNCWLNSSCSWSSSSPRERLLQLVEKGAVDWSVTDNWVRVR 354

BLAST of Pay0013278 vs. ExPASy TrEMBL
Match: A0A0A0KLC9 (Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G490950 PE=3 SV=1)

HSP 1 Score: 679.1 bits (1751), Expect = 1.0e-191
Identity = 321/354 (90.68%), Postives = 337/354 (95.20%), Query Frame = 0

Query: 1   MDWVRGHEIGHGSFATINLATPSSGDRFPPLMAVKSSELVCSSSLKNEKQILDLLGDDCP 60
           MDWVRG EIG+GSFATINLAT  SGDRFPPLMAVKSS LVCS+SLKNEKQ+LDLLGDDCP
Sbjct: 1   MDWVRGDEIGYGSFATINLATSCSGDRFPPLMAVKSSGLVCSASLKNEKQVLDLLGDDCP 60

Query: 61  QIIRCFGESCSVENGEELYNLFLEYATGGSLADRIQSCGGRLPEFEVRRYTRTILEGLRY 120
           QIIRCFG+SCSVENGEELYNLFLEYA+GGSLADRIQS GGRLPEFEVRRYTRTILEGLRY
Sbjct: 61  QIIRCFGDSCSVENGEELYNLFLEYASGGSLADRIQSRGGRLPEFEVRRYTRTILEGLRY 120

Query: 121 IHGKGFVHCDVKPRNILVFGDGDAKIADFGLSKKAGKNRVETGEETGRFQLRGSPLYMSP 180
           IHGKGFVHCDVKPRNILVFGDGDAKIADFGLSKKAGKNRV TGEETG+FQLRGSPLYMSP
Sbjct: 121 IHGKGFVHCDVKPRNILVFGDGDAKIADFGLSKKAGKNRVGTGEETGKFQLRGSPLYMSP 180

Query: 181 ESLNDNEYESPCDIWAVGCAVVEMFTGKPAWNCRPESNVFALLIKIGIGEELPEIPKDLS 240
           ES+NDNEYESPCDIWAVGCAVVEM TGKPAWNCRPESNVFALLIKIGIGEELPEIPKDLS
Sbjct: 181 ESVNDNEYESPCDIWAVGCAVVEMLTGKPAWNCRPESNVFALLIKIGIGEELPEIPKDLS 240

Query: 241 EEGKDFLRKCLVKDPVKRWTADMLLKHPFVADSGRCVPLGEMDEVSTSPRCPFDFEDWAS 300
           EEGKDFL+KCLVKDP+KRWTADMLLKHPFVA+SGRCVPL  ++EVSTSPRCPFDFEDWAS
Sbjct: 241 EEGKDFLKKCLVKDPLKRWTADMLLKHPFVAESGRCVPLAGVEEVSTSPRCPFDFEDWAS 300

Query: 301 IHSQERDPRNEEESNCWLNSSCSWSSSSPRERLLQLVEKGAVDWSVTDNWVRVR 355
           IHSQE DPRNEEE+NCWLN   +W S SPRERLL+LV  GAV+WSVTDNWVRVR
Sbjct: 301 IHSQESDPRNEEEANCWLN---NW-SCSPRERLLELVGNGAVEWSVTDNWVRVR 350

BLAST of Pay0013278 vs. ExPASy TrEMBL
Match: A0A6J1HG32 (mitogen-activated protein kinase kinase kinase 17-like OS=Cucurbita moschata OX=3662 GN=LOC111463177 PE=3 SV=1)

HSP 1 Score: 597.8 bits (1540), Expect = 2.9e-167
Identity = 292/357 (81.79%), Postives = 315/357 (88.24%), Query Frame = 0

Query: 1   MDWVRGHEIGHGSFATINLATPS--SGDRFPPLMAVKSSELVCSSSLKNEKQILDLLGDD 60
           MDW+RGHEIG GSFATINLA PS  S  +FPPLMAVKSS  V S+SLKNEK++LDLLG D
Sbjct: 1   MDWIRGHEIGCGSFATINLAMPSNTSNAQFPPLMAVKSSPAVSSASLKNEKRVLDLLG-D 60

Query: 61  CPQIIRCFGESCSVENGEELYNLFLEYATGGSLADRIQSCGGRLPEFEVRRYTRTILEGL 120
           CPQIIRCFGESCSVE G+ELYNLFLEYA+GGSLADRIQS GG LPEFEVRR TRTILEGL
Sbjct: 61  CPQIIRCFGESCSVERGDELYNLFLEYASGGSLADRIQSHGGWLPEFEVRRLTRTILEGL 120

Query: 121 RYIHGKGFVHCDVKPRNILVFGDGDAKIADFGLSKKAGKNRVETGEETGRFQLRGSPLYM 180
           RYIH KGFVHCDVKPRNILVFGDG+AKIADFGLSKKAGKNR ETGEE G+FQLRGSPLYM
Sbjct: 121 RYIHEKGFVHCDVKPRNILVFGDGEAKIADFGLSKKAGKNRAETGEE-GKFQLRGSPLYM 180

Query: 181 SPESLNDNEYESPCDIWAVGCAVVEMFTGKPAWNCRPESNVFALLIKIGIGEELPEIPKD 240
           SPES+NDNEYESPCDIWAVGCAVVEM TGKPAWN   ESN+++LL+KIGIGEELP+IPK 
Sbjct: 181 SPESVNDNEYESPCDIWAVGCAVVEMLTGKPAWNFPMESNIYSLLLKIGIGEELPKIPKY 240

Query: 241 LSEEGKDFLRKCLVKDPVKRWTADMLLKHPFVADSG-RCVPLGEMDEVSTSPRCPFDFED 300
           +SEEGKDFL KCLVKDP KRWTA+MLL HPF+ADSG   VPL   DE STSPRCPFDFED
Sbjct: 241 ISEEGKDFLGKCLVKDPAKRWTAEMLLNHPFLADSGSSSVPLTPKDEPSTSPRCPFDFED 300

Query: 301 WASIHSQERDPRNEEESNCWLNSSCSWSSSSPRERLLQLVEKGAVDWSVTDNWVRVR 355
           WASIHSQE D + EEESNCWL+SSC W S SP ERLLQLV+KG VDW +TD+WV VR
Sbjct: 301 WASIHSQEVDQKFEEESNCWLDSSCPW-SCSPTERLLQLVDKGPVDWVITDSWVTVR 354

BLAST of Pay0013278 vs. ExPASy TrEMBL
Match: A0A6J1KQ57 (mitogen-activated protein kinase kinase kinase 18-like OS=Cucurbita maxima OX=3661 GN=LOC111497247 PE=3 SV=1)

HSP 1 Score: 586.6 bits (1511), Expect = 6.7e-164
Identity = 286/357 (80.11%), Postives = 311/357 (87.11%), Query Frame = 0

Query: 1   MDWVRGHEIGHGSFATINLATPS--SGDRFPPLMAVKSSELVCSSSLKNEKQILDLLGDD 60
           M+W+RGHEIG GSFATINLA PS  S  +FPPLMAVKSS  V S+SLKNEK++LDLLG D
Sbjct: 1   MEWIRGHEIGCGSFATINLAMPSNTSNAQFPPLMAVKSSPAVSSASLKNEKRVLDLLG-D 60

Query: 61  CPQIIRCFGESCSVENGEELYNLFLEYATGGSLADRIQSCGGRLPEFEVRRYTRTILEGL 120
           CPQIIRCFGESCSVE G+ELYNLFLEYA+GGSLADRIQS GG LPEFEVRR+TRTILEGL
Sbjct: 61  CPQIIRCFGESCSVERGDELYNLFLEYASGGSLADRIQSHGGWLPEFEVRRFTRTILEGL 120

Query: 121 RYIHGKGFVHCDVKPRNILVFGDGDAKIADFGLSKKAGKNRVETGEETGRFQLRGSPLYM 180
           RYIH KGFVHCDVKPRNILVFGDGDAKIADFGLSKKAGKNR ETGEE G+FQLRGSPLYM
Sbjct: 121 RYIHEKGFVHCDVKPRNILVFGDGDAKIADFGLSKKAGKNRAETGEE-GKFQLRGSPLYM 180

Query: 181 SPESLNDNEYESPCDIWAVGCAVVEMFTGKPAWNCRPESNVFALLIKIGIGEELPEIPKD 240
           SPES+ND+EYESPCDIWAVGCAVVEM TGKPAWN   E N+++LL+KIGIGEELP+IPK 
Sbjct: 181 SPESVNDDEYESPCDIWAVGCAVVEMLTGKPAWNFPVECNIYSLLLKIGIGEELPKIPKY 240

Query: 241 LSEEGKDFLRKCLVKDPVKRWTADMLLKHPFVADSG-RCVPLGEMDEVSTSPRCPFDFED 300
           +SEEGKDFL KCLVKDP KRWTA+MLL HPF+ADSG   VPL    E  TSPRCPFDFED
Sbjct: 241 ISEEGKDFLGKCLVKDPAKRWTAEMLLNHPFLADSGSSSVPLTPKGEPLTSPRCPFDFED 300

Query: 301 WASIHSQERDPRNEEESNCWLNSSCSWSSSSPRERLLQLVEKGAVDWSVTDNWVRVR 355
           WASIHS E D + EEESNCWL+SSC W S SP ERLLQLV+ G VDW +TD+WV VR
Sbjct: 301 WASIHSLEMDQKFEEESNCWLDSSCPW-SCSPTERLLQLVDNGPVDWVITDSWVTVR 354

BLAST of Pay0013278 vs. NCBI nr
Match: XP_008440288.1 (PREDICTED: mitogen-activated protein kinase kinase kinase 3-like [Cucumis melo] >TYK12881.1 mitogen-activated protein kinase kinase kinase 3-like [Cucumis melo var. makuwa])

HSP 1 Score: 751.9 bits (1940), Expect = 2.5e-213
Identity = 354/354 (100.00%), Postives = 354/354 (100.00%), Query Frame = 0

Query: 1   MDWVRGHEIGHGSFATINLATPSSGDRFPPLMAVKSSELVCSSSLKNEKQILDLLGDDCP 60
           MDWVRGHEIGHGSFATINLATPSSGDRFPPLMAVKSSELVCSSSLKNEKQILDLLGDDCP
Sbjct: 1   MDWVRGHEIGHGSFATINLATPSSGDRFPPLMAVKSSELVCSSSLKNEKQILDLLGDDCP 60

Query: 61  QIIRCFGESCSVENGEELYNLFLEYATGGSLADRIQSCGGRLPEFEVRRYTRTILEGLRY 120
           QIIRCFGESCSVENGEELYNLFLEYATGGSLADRIQSCGGRLPEFEVRRYTRTILEGLRY
Sbjct: 61  QIIRCFGESCSVENGEELYNLFLEYATGGSLADRIQSCGGRLPEFEVRRYTRTILEGLRY 120

Query: 121 IHGKGFVHCDVKPRNILVFGDGDAKIADFGLSKKAGKNRVETGEETGRFQLRGSPLYMSP 180
           IHGKGFVHCDVKPRNILVFGDGDAKIADFGLSKKAGKNRVETGEETGRFQLRGSPLYMSP
Sbjct: 121 IHGKGFVHCDVKPRNILVFGDGDAKIADFGLSKKAGKNRVETGEETGRFQLRGSPLYMSP 180

Query: 181 ESLNDNEYESPCDIWAVGCAVVEMFTGKPAWNCRPESNVFALLIKIGIGEELPEIPKDLS 240
           ESLNDNEYESPCDIWAVGCAVVEMFTGKPAWNCRPESNVFALLIKIGIGEELPEIPKDLS
Sbjct: 181 ESLNDNEYESPCDIWAVGCAVVEMFTGKPAWNCRPESNVFALLIKIGIGEELPEIPKDLS 240

Query: 241 EEGKDFLRKCLVKDPVKRWTADMLLKHPFVADSGRCVPLGEMDEVSTSPRCPFDFEDWAS 300
           EEGKDFLRKCLVKDPVKRWTADMLLKHPFVADSGRCVPLGEMDEVSTSPRCPFDFEDWAS
Sbjct: 241 EEGKDFLRKCLVKDPVKRWTADMLLKHPFVADSGRCVPLGEMDEVSTSPRCPFDFEDWAS 300

Query: 301 IHSQERDPRNEEESNCWLNSSCSWSSSSPRERLLQLVEKGAVDWSVTDNWVRVR 355
           IHSQERDPRNEEESNCWLNSSCSWSSSSPRERLLQLVEKGAVDWSVTDNWVRVR
Sbjct: 301 IHSQERDPRNEEESNCWLNSSCSWSSSSPRERLLQLVEKGAVDWSVTDNWVRVR 354

BLAST of Pay0013278 vs. NCBI nr
Match: XP_011657865.1 (mitogen-activated protein kinase kinase kinase 17 [Cucumis sativus] >KGN48531.1 hypothetical protein Csa_004223 [Cucumis sativus])

HSP 1 Score: 679.1 bits (1751), Expect = 2.1e-191
Identity = 321/354 (90.68%), Postives = 337/354 (95.20%), Query Frame = 0

Query: 1   MDWVRGHEIGHGSFATINLATPSSGDRFPPLMAVKSSELVCSSSLKNEKQILDLLGDDCP 60
           MDWVRG EIG+GSFATINLAT  SGDRFPPLMAVKSS LVCS+SLKNEKQ+LDLLGDDCP
Sbjct: 1   MDWVRGDEIGYGSFATINLATSCSGDRFPPLMAVKSSGLVCSASLKNEKQVLDLLGDDCP 60

Query: 61  QIIRCFGESCSVENGEELYNLFLEYATGGSLADRIQSCGGRLPEFEVRRYTRTILEGLRY 120
           QIIRCFG+SCSVENGEELYNLFLEYA+GGSLADRIQS GGRLPEFEVRRYTRTILEGLRY
Sbjct: 61  QIIRCFGDSCSVENGEELYNLFLEYASGGSLADRIQSRGGRLPEFEVRRYTRTILEGLRY 120

Query: 121 IHGKGFVHCDVKPRNILVFGDGDAKIADFGLSKKAGKNRVETGEETGRFQLRGSPLYMSP 180
           IHGKGFVHCDVKPRNILVFGDGDAKIADFGLSKKAGKNRV TGEETG+FQLRGSPLYMSP
Sbjct: 121 IHGKGFVHCDVKPRNILVFGDGDAKIADFGLSKKAGKNRVGTGEETGKFQLRGSPLYMSP 180

Query: 181 ESLNDNEYESPCDIWAVGCAVVEMFTGKPAWNCRPESNVFALLIKIGIGEELPEIPKDLS 240
           ES+NDNEYESPCDIWAVGCAVVEM TGKPAWNCRPESNVFALLIKIGIGEELPEIPKDLS
Sbjct: 181 ESVNDNEYESPCDIWAVGCAVVEMLTGKPAWNCRPESNVFALLIKIGIGEELPEIPKDLS 240

Query: 241 EEGKDFLRKCLVKDPVKRWTADMLLKHPFVADSGRCVPLGEMDEVSTSPRCPFDFEDWAS 300
           EEGKDFL+KCLVKDP+KRWTADMLLKHPFVA+SGRCVPL  ++EVSTSPRCPFDFEDWAS
Sbjct: 241 EEGKDFLKKCLVKDPLKRWTADMLLKHPFVAESGRCVPLAGVEEVSTSPRCPFDFEDWAS 300

Query: 301 IHSQERDPRNEEESNCWLNSSCSWSSSSPRERLLQLVEKGAVDWSVTDNWVRVR 355
           IHSQE DPRNEEE+NCWLN   +W S SPRERLL+LV  GAV+WSVTDNWVRVR
Sbjct: 301 IHSQESDPRNEEEANCWLN---NW-SCSPRERLLELVGNGAVEWSVTDNWVRVR 350

BLAST of Pay0013278 vs. NCBI nr
Match: XP_038883122.1 (mitogen-activated protein kinase kinase kinase 17-like [Benincasa hispida])

HSP 1 Score: 660.2 bits (1702), Expect = 9.9e-186
Identity = 313/357 (87.68%), Postives = 328/357 (91.88%), Query Frame = 0

Query: 1   MDWVRGHEIGHGSFATINLATPS---SGDRFPPLMAVKSSELVCSSSLKNEKQILDLLGD 60
           M+WVRG EIGHGSFATIN ATPS   SG +FPPLMAVKSS +VCS+SLKNEKQ+LDLLGD
Sbjct: 1   MEWVRGDEIGHGSFATINFATPSKTYSGHQFPPLMAVKSSGVVCSASLKNEKQVLDLLGD 60

Query: 61  DCPQIIRCFGESCSVENGEELYNLFLEYATGGSLADRIQSCGGRLPEFEVRRYTRTILEG 120
           DCPQIIRCFGESCS ENGEE+YNLFLEYA+GGSLADRIQS GGRLPEFEVRRYTRTILEG
Sbjct: 61  DCPQIIRCFGESCSYENGEEIYNLFLEYASGGSLADRIQSRGGRLPEFEVRRYTRTILEG 120

Query: 121 LRYIHGKGFVHCDVKPRNILVFGDGDAKIADFGLSKKAGKNRVETGEETGRFQLRGSPLY 180
           LRYIHGKGFVHCDVKPRNILVF DGDAKIADFGLSKKAGK R ETGEE GRFQLRGSPLY
Sbjct: 121 LRYIHGKGFVHCDVKPRNILVFDDGDAKIADFGLSKKAGKKRAETGEEAGRFQLRGSPLY 180

Query: 181 MSPESLNDNEYESPCDIWAVGCAVVEMFTGKPAWNCRPESNVFALLIKIGIGEELPEIPK 240
           MSPES+ DNEYESPCDIWAVGCAVVEM TGKPAWNCRPESN+++LL+KIGIGEELPEIPK
Sbjct: 181 MSPESVKDNEYESPCDIWAVGCAVVEMLTGKPAWNCRPESNIYSLLLKIGIGEELPEIPK 240

Query: 241 DLSEEGKDFLRKCLVKDPVKRWTADMLLKHPFVADSGRCVPLGEMDEVSTSPRCPFDFED 300
           DLSEEGKDFL KCLVKDP KRWTADMLLKHPFVADSGR VPL  +DE STSPRCPFDFED
Sbjct: 241 DLSEEGKDFLGKCLVKDPAKRWTADMLLKHPFVADSGRTVPLTAVDEPSTSPRCPFDFED 300

Query: 301 WASIHSQERDPRNEEESNCWLNSSCSWSSSSPRERLLQLVEKGAVDWSVTDNWVRVR 355
           WASIHSQE D R EEESNCWLNSSCSW  SSPRERLLQLV+ G VDWSVT+NWV VR
Sbjct: 301 WASIHSQEADQRFEEESNCWLNSSCSW-LSSPRERLLQLVDNGPVDWSVTNNWVTVR 356

BLAST of Pay0013278 vs. NCBI nr
Match: XP_022962795.1 (mitogen-activated protein kinase kinase kinase 17-like [Cucurbita moschata])

HSP 1 Score: 597.8 bits (1540), Expect = 6.0e-167
Identity = 292/357 (81.79%), Postives = 315/357 (88.24%), Query Frame = 0

Query: 1   MDWVRGHEIGHGSFATINLATPS--SGDRFPPLMAVKSSELVCSSSLKNEKQILDLLGDD 60
           MDW+RGHEIG GSFATINLA PS  S  +FPPLMAVKSS  V S+SLKNEK++LDLLG D
Sbjct: 1   MDWIRGHEIGCGSFATINLAMPSNTSNAQFPPLMAVKSSPAVSSASLKNEKRVLDLLG-D 60

Query: 61  CPQIIRCFGESCSVENGEELYNLFLEYATGGSLADRIQSCGGRLPEFEVRRYTRTILEGL 120
           CPQIIRCFGESCSVE G+ELYNLFLEYA+GGSLADRIQS GG LPEFEVRR TRTILEGL
Sbjct: 61  CPQIIRCFGESCSVERGDELYNLFLEYASGGSLADRIQSHGGWLPEFEVRRLTRTILEGL 120

Query: 121 RYIHGKGFVHCDVKPRNILVFGDGDAKIADFGLSKKAGKNRVETGEETGRFQLRGSPLYM 180
           RYIH KGFVHCDVKPRNILVFGDG+AKIADFGLSKKAGKNR ETGEE G+FQLRGSPLYM
Sbjct: 121 RYIHEKGFVHCDVKPRNILVFGDGEAKIADFGLSKKAGKNRAETGEE-GKFQLRGSPLYM 180

Query: 181 SPESLNDNEYESPCDIWAVGCAVVEMFTGKPAWNCRPESNVFALLIKIGIGEELPEIPKD 240
           SPES+NDNEYESPCDIWAVGCAVVEM TGKPAWN   ESN+++LL+KIGIGEELP+IPK 
Sbjct: 181 SPESVNDNEYESPCDIWAVGCAVVEMLTGKPAWNFPMESNIYSLLLKIGIGEELPKIPKY 240

Query: 241 LSEEGKDFLRKCLVKDPVKRWTADMLLKHPFVADSG-RCVPLGEMDEVSTSPRCPFDFED 300
           +SEEGKDFL KCLVKDP KRWTA+MLL HPF+ADSG   VPL   DE STSPRCPFDFED
Sbjct: 241 ISEEGKDFLGKCLVKDPAKRWTAEMLLNHPFLADSGSSSVPLTPKDEPSTSPRCPFDFED 300

Query: 301 WASIHSQERDPRNEEESNCWLNSSCSWSSSSPRERLLQLVEKGAVDWSVTDNWVRVR 355
           WASIHSQE D + EEESNCWL+SSC W S SP ERLLQLV+KG VDW +TD+WV VR
Sbjct: 301 WASIHSQEVDQKFEEESNCWLDSSCPW-SCSPTERLLQLVDKGPVDWVITDSWVTVR 354

BLAST of Pay0013278 vs. NCBI nr
Match: KAG7027055.1 (Mitogen-activated protein kinase kinase kinase 17, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 596.7 bits (1537), Expect = 1.3e-166
Identity = 291/357 (81.51%), Postives = 314/357 (87.96%), Query Frame = 0

Query: 1   MDWVRGHEIGHGSFATINLATPS--SGDRFPPLMAVKSSELVCSSSLKNEKQILDLLGDD 60
           MDW+RGHEIG GSFATINLA PS  S  +FPPLMAVKSS  V S+SLKNEK++LDLLG D
Sbjct: 1   MDWIRGHEIGCGSFATINLAMPSNTSNAQFPPLMAVKSSPAVSSASLKNEKRVLDLLG-D 60

Query: 61  CPQIIRCFGESCSVENGEELYNLFLEYATGGSLADRIQSCGGRLPEFEVRRYTRTILEGL 120
           CPQIIRCFGESCSVE G+ELYNLFLEYA+GGSLADRIQS GG LPEFEVRR TRTILEGL
Sbjct: 61  CPQIIRCFGESCSVERGDELYNLFLEYASGGSLADRIQSHGGWLPEFEVRRLTRTILEGL 120

Query: 121 RYIHGKGFVHCDVKPRNILVFGDGDAKIADFGLSKKAGKNRVETGEETGRFQLRGSPLYM 180
           RYIH KGFVHCDVKPRNILVFGDG+AKIADFGLSKKAGKNR ETGEE G+FQLRGSPLYM
Sbjct: 121 RYIHDKGFVHCDVKPRNILVFGDGEAKIADFGLSKKAGKNRAETGEE-GKFQLRGSPLYM 180

Query: 181 SPESLNDNEYESPCDIWAVGCAVVEMFTGKPAWNCRPESNVFALLIKIGIGEELPEIPKD 240
           SPES+NDNEYESPCDIWAVGCAVVEM TGKPAWN   ESN+++LL+KIGIGEELP+IPK 
Sbjct: 181 SPESVNDNEYESPCDIWAVGCAVVEMLTGKPAWNFSMESNIYSLLLKIGIGEELPKIPKY 240

Query: 241 LSEEGKDFLRKCLVKDPVKRWTADMLLKHPFVADSG-RCVPLGEMDEVSTSPRCPFDFED 300
           +SEEGKDFL KCLVKDP KRWTA+MLL HPF+ADSG   VPL   DE STSPRCPFDFED
Sbjct: 241 ISEEGKDFLGKCLVKDPAKRWTAEMLLNHPFLADSGSSSVPLTPKDEPSTSPRCPFDFED 300

Query: 301 WASIHSQERDPRNEEESNCWLNSSCSWSSSSPRERLLQLVEKGAVDWSVTDNWVRVR 355
           WASIHSQE D + EEESNCWL+SSC W S SP ERLLQLV+ G VDW +TD+WV VR
Sbjct: 301 WASIHSQEVDQKFEEESNCWLDSSCPW-SCSPTERLLQLVDNGPVDWVITDSWVTVR 354

BLAST of Pay0013278 vs. TAIR 10
Match: AT4G36950.1 (mitogen-activated protein kinase kinase kinase 21 )

HSP 1 Score: 391.0 bits (1003), Expect = 1.0e-108
Identity = 200/362 (55.25%), Postives = 257/362 (70.99%), Query Frame = 0

Query: 1   MDWVRGHEIGHGSFATINLATP--SSGDRFPPLMAVKSSELVCSSSLKNEKQILDLLGDD 60
           M+W+R   IGHGSF+T++LAT   SS   FP LMAVKSS +VCS++L+NE+ +LD LG D
Sbjct: 1   MEWIRRETIGHGSFSTVSLATTSGSSSKAFPSLMAVKSSGVVCSAALRNERDVLDDLG-D 60

Query: 61  CPQIIRCFGESCSVENGEELYNLFLEYATGGSLADRIQSCGGRLPEFEVRRYTRTILEGL 120
           C +I+RCFGE  +VENGEE+YNLFLEYA+GGSLADRI+S G  LPEFEVRR+TR+I++GL
Sbjct: 61  CSEIVRCFGEGRTVENGEEIYNLFLEYASGGSLADRIKSSGEALPEFEVRRFTRSIVKGL 120

Query: 121 RYIHGKGFVHCDVKPRNILVFGDGDAKIADFGLSKKAGKNRVETGEETGRFQLRGSPLYM 180
            +IHG GF HCD+K  N+LVFGDGD KI+DFGL+K+       +GE     ++RG+PLYM
Sbjct: 121 CHIHGNGFTHCDIKLENVLVFGDGDVKISDFGLAKR------RSGEVC--VEIRGTPLYM 180

Query: 181 SPESLNDNEYESPCDIWAVGCAVVEMFTGKPAWNCRPE---SNVFALLIKIGIGEELPEI 240
           +PES+N  E+ESP DIWA+GC+VVEM +GK AW C  +   +NV +LL++IG G+E+P I
Sbjct: 181 APESVNHGEFESPADIWALGCSVVEMSSGKTAW-CLEDGVMNNVMSLLVRIGSGDEVPRI 240

Query: 241 PKDLSEEGKDFLRKCLVKDPVKRWTADMLLKHPFVADSGRCVPLGEMDEV-STSPRCPFD 300
           P +LSEEGKDF+ KC VK+  +RWTA+MLL HPF+A        GE DE  S SPR PFD
Sbjct: 241 PVELSEEGKDFVSKCFVKNAAERWTAEMLLDHPFLAVDDES---GEEDEACSVSPRNPFD 300

Query: 301 FEDWASIHSQERDPRNEEESNCWLNSSCSWSS--SSPRERLLQLVEKGAVDWSVTDNWVR 355
           F  W S+ S              +N S  + S   SP ER+  LV +   DWSV+ +WV 
Sbjct: 301 FPGWNSVQSP-------------VNDSVMFGSLVGSPEERISGLVSEKVPDWSVSCDWVN 336

BLAST of Pay0013278 vs. TAIR 10
Match: AT5G67080.1 (mitogen-activated protein kinase kinase kinase 19 )

HSP 1 Score: 387.1 bits (993), Expect = 1.5e-107
Identity = 195/366 (53.28%), Postives = 253/366 (69.13%), Query Frame = 0

Query: 1   MDWVRGHEIGHGSFATINLATPSSGD--RFPPLMAVKSSELVCSSSLKNEKQILDLLGDD 60
           M+W+RG  IG+G+F+T++LAT S+ D   FPPLMAVKS++   ++SL NEK +LD LGDD
Sbjct: 1   MEWIRGETIGYGTFSTVSLATRSNNDSGEFPPLMAVKSADSYGAASLANEKSVLDNLGDD 60

Query: 61  CPQIIRCFGESCSVENGEELYNLFLEYATGGSLADRIQSCGGR-LPEFEVRRYTRTILEG 120
           C +I+RCFGE  +VENGEE++NLFLEYA+ GSL   ++   G  +PE  VRR+T ++L G
Sbjct: 61  CNEIVRCFGEDRTVENGEEMHNLFLEYASRGSLESYLKKLAGEGVPESTVRRHTGSVLRG 120

Query: 121 LRYIHGKGFVHCDVKPRNILVFGDGDAKIADFGLSKKAGKNRVETGEETGRFQLRGSPLY 180
           LR+IH  GF HCD+K  NIL+FGDG  KIADFGL+K+ G     T    G  Q+RG+PLY
Sbjct: 121 LRHIHANGFAHCDLKLGNILLFGDGAVKIADFGLAKRIGD---LTALNYG-VQIRGTPLY 180

Query: 181 MSPESLNDNEYESPCDIWAVGCAVVEMFTGKPAWNCRPESNVFALLIKIGIGEELPEIPK 240
           M+PES+NDNEY S  D+WA+GC VVEMF+GK AW+ +  SN  +LL++IG+G+E+P IP+
Sbjct: 181 MAPESVNDNEYGSEGDVWALGCVVVEMFSGKTAWSLKEGSNFMSLLLRIGVGDEVPMIPE 240

Query: 241 DLSEEGKDFLRKCLVKDPVKRWTADMLLKHPFV-ADSGRCVPLGEMD--------EVSTS 300
           +LSE+G+DFL KC VKDP KRWTA+MLL HPFV  D    V + E D        +VSTS
Sbjct: 241 ELSEQGRDFLSKCFVKDPKKRWTAEMLLNHPFVTVDVDHDVLVKEEDFVVNMKTEDVSTS 300

Query: 301 PRCPFDFEDWASIHSQERDPRNEEESNCWLNSSCSWSSSSPRERLLQLVEKGAVDWSVTD 355
           PRCPF+F DW S+                  SS S +  SP ER+  LV     DWSVT+
Sbjct: 301 PRCPFEFPDWVSV------------------SSGSQTIDSPDERVASLVTDMIPDWSVTN 344

BLAST of Pay0013278 vs. TAIR 10
Match: AT3G50310.1 (mitogen-activated protein kinase kinase kinase 20 )

HSP 1 Score: 367.5 bits (942), Expect = 1.2e-101
Identity = 189/364 (51.92%), Postives = 247/364 (67.86%), Query Frame = 0

Query: 1   MDWVRGHEIGHGSFATINLATPS--SGDRFPPLMAVKSSELVCSSSLKNEKQILDLLGDD 60
           M+WVRG  IG G+F+T++ AT S  SGD FP L+AVKS++   ++SL NEK +LD LG D
Sbjct: 1   MEWVRGETIGFGTFSTVSTATKSRNSGD-FPALIAVKSTDAYGAASLSNEKSVLDSLG-D 60

Query: 61  CPQIIRCFGESCSVENGEELYNLFLEYATGGSLADRIQSCGGR-LPEFEVRRYTRTILEG 120
           CP+IIRC+GE  +VENGEE++NL LEYA+ GSLA  ++  GG  LPE  VRR+T ++L G
Sbjct: 61  CPEIIRCYGEDSTVENGEEMHNLLLEYASRGSLASYMKKLGGEGLPESTVRRHTGSVLRG 120

Query: 121 LRYIHGKGFVHCDVKPRNILVFGDGDAKIADFGLSKKAGKNRVETGEETGRFQLRGSPLY 180
           LR+IH KGF HCD+K  NIL+F DG  KIADFGL+ +   +     +     ++RG+PLY
Sbjct: 121 LRHIHAKGFAHCDIKLANILLFNDGSVKIADFGLAMRVDGDLTALRKSV---EIRGTPLY 180

Query: 181 MSPESLNDNEYESPCDIWAVGCAVVEMFTGKPAWNCRPESNVFALLIKIGIGEELPEIPK 240
           M+PE +NDNEY S  D+WA+GCAVVEMF+GK AW+ +  S+  +LLI+IG+G+ELP+IP+
Sbjct: 181 MAPECVNDNEYGSAADVWALGCAVVEMFSGKTAWSVKEGSHFMSLLIRIGVGDELPKIPE 240

Query: 241 DLSEEGKDFLRKCLVKDPVKRWTADMLLKHPFVA----DSGR---CVPLGEMDEVSTSPR 300
            LSEEGKDFL KC VKDP KRWTA+MLL H FV     D  R    V + + D+V  SP+
Sbjct: 241 MLSEEGKDFLSKCFVKDPAKRWTAEMLLNHSFVTIDLEDDHRENFVVKVKDEDKVLMSPK 300

Query: 301 CPFDFEDWASIHSQERDPRNEEESNCWLNSSCSWSSSSPRERLLQLVEKGAVDWSVTDNW 355
           CPF+F+DW S  + + +P                S  SP ERL  LV     DWSV  +W
Sbjct: 301 CPFEFDDWDSF-TLDSNP----------------SFDSPVERLGSLVSGSIPDWSVGGSW 342

BLAST of Pay0013278 vs. TAIR 10
Match: AT5G55090.1 (mitogen-activated protein kinase kinase kinase 15 )

HSP 1 Score: 254.2 bits (648), Expect = 1.5e-67
Identity = 149/366 (40.71%), Postives = 206/366 (56.28%), Query Frame = 0

Query: 2   DWVRGHEIGHGSFATINLATPSSGDRFPPLMAVKSSELVCSSSLKNEKQILDLLGDDCPQ 61
           +W+RG  IG GS AT++L   +SGD F    AVKS+E   S+ L+ E+ IL  L    P 
Sbjct: 5   NWIRGPIIGRGSTATVSLGITNSGDFF----AVKSAEFSSSAFLQREQSILSKLSS--PY 64

Query: 62  IIRCFGESCSVENGEELYNLFLEYATGGSLADRIQSCGGRLPEFEVRRYTRTILEGLRYI 121
           I++  G + + EN + +YNL +EY +GGSL D I++ GG+LPE  +R YTR IL+GL Y+
Sbjct: 65  IVKYIGSNVTKENDKLMYNLLMEYVSGGSLHDLIKNSGGKLPEPLIRSYTRQILKGLMYL 124

Query: 122 HGKGFVHCDVKPRNILVFGDGDAKIADFGLSKKAGKNRVETGEETGRFQLRGSPLYMSPE 181
           H +G VHCDVK +N+++ G+  AKI D G +K        T EE    +  G+P +MSPE
Sbjct: 125 HDQGIVHCDVKSQNVMIGGE-IAKIVDLGCAK--------TVEENENLEFSGTPAFMSPE 184

Query: 182 SLNDNEYESPCDIWAVGCAVVEMFTGKPAWNCRPESN-VFALLIKIGIGEELPEIPKDLS 241
                E   P D+WA+GC V+EM TG   W   PE N V A + KIG   E P IP  LS
Sbjct: 185 VARGEEQSFPADVWALGCTVIEMATGSSPW---PELNDVVAAIYKIGFTGESPVIPVWLS 244

Query: 242 EEGKDFLRKCLVKDPVKRWTADMLLKHPFVAD-------SGRCVPLGEMDEVSTSPRCPF 301
           E+G+DFLRKCL KDP +RWT + LL+HPF+ +       +G C+        S+SP    
Sbjct: 245 EKGQDFLRKCLRKDPKQRWTVEELLQHPFLDEEDNDSDQTGNCLN-------SSSPSTVL 304

Query: 302 DFEDWASIHSQERDPRNEEESNCWLNS-SCSWSSSS-PRERLLQLV---EKGAVDWSVTD 355
           D   W    +       E+  + + NS +  W   S P +R+ +L      G  DW  T+
Sbjct: 305 DQRFWDLCETSRSRFIKEDHEDPFANSTNFLWDDDSLPGDRIKKLAGDESSGEPDWE-TN 344

BLAST of Pay0013278 vs. TAIR 10
Match: AT4G26890.1 (mitogen-activated protein kinase kinase kinase 16 )

HSP 1 Score: 244.2 bits (622), Expect = 1.6e-64
Identity = 136/358 (37.99%), Postives = 198/358 (55.31%), Query Frame = 0

Query: 1   MDWVRGHEIGHGSFATINLATPSSGDRFPPLMAVKSSELVCSSSLKNEKQILDLLGDDCP 60
           ++W RG  IG GS AT+++A  SSG+    L AVKS++L  SS L+ E+ IL  L    P
Sbjct: 3   INWTRGPIIGRGSTATVSIAISSSGE----LFAVKSADLSSSSLLQKEQSILSTLSS--P 62

Query: 61  QIIRCFGESCSVENGEELYNLFLEYATGGSLADRIQSCGGRLPEFEVRRYTRTILEGLRY 120
            +++  G   + E+   +YN+ +EY +GG+L D I++ GG+LPE E+R YTR IL GL Y
Sbjct: 63  HMVKYIGTGLTRESNGLVYNILMEYVSGGNLHDLIKNSGGKLPEPEIRSYTRQILNGLVY 122

Query: 121 IHGKGFVHCDVKPRNILVFGDGDAKIADFGLSKKAGKNRVETGEETGRFQLRGSPLYMSP 180
           +H +G VHCD+K  N+LV  +G  KIAD G +K   K+           +  G+P +M+P
Sbjct: 123 LHERGIVHCDLKSHNVLVEENGVLKIADMGCAKSVDKS-----------EFSGTPAFMAP 182

Query: 181 ESLNDNEYESPCDIWAVGCAVVEMFTGKPAWNCRPESN-VFALLIKIGIGEELPEIPKDL 240
           E     E   P D+WA+GC ++EM TG   W   PE N V A + KIG   E P IP  +
Sbjct: 183 EVARGEEQRFPADVWALGCTMIEMMTGSSPW---PELNDVVAAMYKIGFSGESPAIPAWI 242

Query: 241 SEEGKDFLRKCLVKDPVKRWTADMLLKHPFVADSGRCVPLGEMDEVSTSPRCPFDFEDWA 300
           S++ KDFL+ CL +D  +RWT + LLKHPF+ D         +   ++SP    D   W 
Sbjct: 243 SDKAKDFLKNCLKEDQKQRWTVEELLKHPFLDDDEESQTSDCLKNKTSSPSTVLDQRFWD 302

Query: 301 SIHSQERDPRNEEESNCWLNSSCSWSSSSPR-ERLLQLVEKGAVDWSVTDN--WVRVR 355
           S  S +    + +  + +   S S  S + R E+L        +DW   D+  W++VR
Sbjct: 303 SCESSKSHLVSIDHEDPFAEYSESLDSPADRIEKLAGDEFSSLLDWDTEDDGGWIQVR 340

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SND61.7e-10051.92Mitogen-activated protein kinase kinase kinase 20 OS=Arabidopsis thaliana OX=370... [more]
O808881.1e-5938.23Mitogen-activated protein kinase kinase kinase 17 OS=Arabidopsis thaliana OX=370... [more]
Q9ZVP51.3e-5237.16Mitogen-activated protein kinase kinase kinase 18 OS=Arabidopsis thaliana OX=370... [more]
F4HRJ43.9e-4436.46Mitogen-activated protein kinase kinase kinase 3 OS=Arabidopsis thaliana OX=3702... [more]
O220421.3e-4237.05Mitogen-activated protein kinase kinase kinase 3 OS=Arabidopsis thaliana OX=3702... [more]
Match NameE-valueIdentityDescription
A0A5D3CM361.2e-213100.00Mitogen-activated protein kinase kinase kinase 3-like OS=Cucumis melo var. makuw... [more]
A0A1S3B1I01.2e-213100.00mitogen-activated protein kinase kinase kinase 3-like OS=Cucumis melo OX=3656 GN... [more]
A0A0A0KLC91.0e-19190.68Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G490... [more]
A0A6J1HG322.9e-16781.79mitogen-activated protein kinase kinase kinase 17-like OS=Cucurbita moschata OX=... [more]
A0A6J1KQ576.7e-16480.11mitogen-activated protein kinase kinase kinase 18-like OS=Cucurbita maxima OX=36... [more]
Match NameE-valueIdentityDescription
XP_008440288.12.5e-213100.00PREDICTED: mitogen-activated protein kinase kinase kinase 3-like [Cucumis melo] ... [more]
XP_011657865.12.1e-19190.68mitogen-activated protein kinase kinase kinase 17 [Cucumis sativus] >KGN48531.1 ... [more]
XP_038883122.19.9e-18687.68mitogen-activated protein kinase kinase kinase 17-like [Benincasa hispida][more]
XP_022962795.16.0e-16781.79mitogen-activated protein kinase kinase kinase 17-like [Cucurbita moschata][more]
KAG7027055.11.3e-16681.51Mitogen-activated protein kinase kinase kinase 17, partial [Cucurbita argyrosper... [more]
Match NameE-valueIdentityDescription
AT4G36950.11.0e-10855.25mitogen-activated protein kinase kinase kinase 21 [more]
AT5G67080.11.5e-10753.28mitogen-activated protein kinase kinase kinase 19 [more]
AT3G50310.11.2e-10151.92mitogen-activated protein kinase kinase kinase 20 [more]
AT5G55090.11.5e-6740.71mitogen-activated protein kinase kinase kinase 15 [more]
AT4G26890.11.6e-6437.99mitogen-activated protein kinase kinase kinase 16 [more]
InterPro
Analysis Name: InterPro Annotations of Melon (Payzawat) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 3..270
e-value: 4.6E-69
score: 245.4
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 5..270
e-value: 8.3E-53
score: 179.4
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 3..270
score: 39.156139
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 93..316
e-value: 3.2E-55
score: 189.2
NoneNo IPR availableGENE3D3.30.200.20Phosphorylase Kinase; domain 1coord: 1..92
e-value: 3.1E-8
score: 35.7
NoneNo IPR availablePIRSRPIRSR630616-1PIRSR630616-1coord: 59..273
e-value: 2.7E-30
score: 103.2
NoneNo IPR availablePIRSRPIRSR000556-1PIRSR000556-1coord: 45..265
e-value: 2.1E-9
score: 34.3
NoneNo IPR availablePIRSRPIRSR630220-2PIRSR630220-2coord: 82..261
e-value: 4.4E-14
score: 49.4
NoneNo IPR availablePANTHERPTHR48011:SF18MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE 20coord: 1..320
NoneNo IPR availablePANTHERPTHR48011CCR4-NOT TRANSCRIPTIONAL COMPLEX SUBUNIT CAF120-RELATEDcoord: 1..320
NoneNo IPR availableCDDcd06606STKc_MAPKKKcoord: 2..270
e-value: 3.69987E-120
score: 345.274
IPR017441Protein kinase, ATP binding sitePROSITEPS00107PROTEIN_KINASE_ATPcoord: 9..35
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 126..138
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 3..297

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Pay0013278.1Pay0013278.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
molecular_function GO:0005524 ATP binding
molecular_function GO:0004674 protein serine/threonine kinase activity
molecular_function GO:0004672 protein kinase activity