Pay0006576 (gene) Melon (Payzawat) v1

Overview
NamePay0006576
Typegene
OrganismCucumis melo L. var. inodorus cv. Payzawat (Melon (Payzawat) v1)
DescriptionPectinesterase
Locationchr05: 29052345 .. 29054533 (-)
RNA-Seq ExpressionPay0006576
SyntenyPay0006576
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTAATCACTGAAGTCATTGGGATAATATGCATCCATGCAGAGAATTCTCAAAAAGAAAATGAACCCTTAACTCCTCTGAAATATTTCAAGATCAAAGGTTACATTCCTGTCTTTGATACTGCAATTAAACTCTTCTTCTAAATTCTAATGGTTCTTTTACATGAATAGCATCCCATCTCGTCATCTCATCAGTGAAAACAAAATCAAATAAGATCATGGATGATTCCCAAAATAATTTGCATTTATGAATTAATATCTCTTTCTTTCTATGTATATGTATGAATACAAGGCCTAATAACTCTTCAGTTTTTACAACTATTCTGATGATAAACGACTTATACTTTCTAACTGGCATCTAAATTCCAAAAAAAAAAAAAAGATTGTAAAAAAAAGTACACCTCCATTTCTCTTGCTCTTCACTCTGTTTAATGCTGTTGTCTTTGGTTTTGAAGCTGTGAATCATGCAAGAGCCTTTGACCTTACAGTTCAATCCGTTGTTGTTGTTGACAAGTCGGGAAGAGGCGATTTCCAGACCGTTCAAGCAGCCATTGACTCAGTTCCACCTAATAACAATCAATGGATTAAGATTCAAATAAACCCTGGTGTATACAAGTAATACTAAAACAGTACCATTATGAAATTTGGTATTGAGGTTTTGTTCTAATATTGTTGGCAAACCTTTTGCAGAGAAAAGGTGACAATCCCACCAGAAAAACCTTTCATATATCTCGAAGGAGCTGACCGCAGCAATACGATCATCACATTCGATGACCATCAACAAACGGACACTAGTGCTACATTTACTTCACGTCCTCCTAACATTATTGCTAGAGGCATTACTTTTGAGGTGCTGTGGCTTCTAAAAACTGAATTTATATTCATAGCATCATTTGAGATTTTGAAGCCCAAGTTAAGTTCATAGTTAATGATATATATCGTATGGCGTACGCACCTGAGAGGGGTTTGAGCAGGATTCTATAACTCTCAATGTCTGTCTTGTTATAGAATTGTTGCTTTTGTATGGTAAATCAAGATGTTTGATTATGTGTTATTGTGATGCACACAGAACTCCTTCAACCTTGAAGAAGCTCCAGAGCTGTTTGTTTGCGATGATGGAACTTACATTACACAAGCAATAGCAGCAAGAATCTATGGCGACAAATCGGCGTTTTTCAACTGTGGATTCAAAGGGTATCAAGACACGTTATGGGATGTGCAAGGTCGCCATTTCTTCCAAAATTGCTACATTGAAGGAGCCATAGACTTCATCTTTGGTAGTGGCCAATCAGTTTATAAGGTACTCAGTTCAAATGTCAATGTTTCTGTTATTTACACCATTGGAAGAGACTTTTACGGCAACTTGGGACCATAAAAACATGTTCTTTTGACAATGTAGGATTGCTTGATAAATGTTAATGTTGCAAGTCTCCCTCAAGTCCACCAAGGATTCATTACTGCACAAGGTCGAAGATCAGCAGCAGATCCAAGTGGATTTGTATTTAAAGGGTGCACCATAACGGGTAGTGGCAAAGCTTTGTTGGGGAGAGCATATGGTCCTTTTTCCAGAGTTATATTCAAAGATGCTATAATGGGTCCAGTGGTAGCACCAGAGGGATGGGACGCCTGGAACTTCAAGAGCAAAGAGTAAGTTCACAGCCAGTTTGTTTTTCAAGCTCAAGCACAGTGAAGCATAATAGTCATTTAGGACTTAAGCGAGGCACAAAAAAAGCAGTTATTATTTATTATTTATTTATTTTTGAGGCGCATTATATATCAAAGTAATGCATAAAGAAGAGAACACAAGAAAATTTGCATTAGGCATAGTAAATTTAATTTTTTTCTGGTTAATAAAGAATGAAAAATCCCCAAGAAACAAGGCTTTGAAGCTTGGGGCTTCACAAAAGGTCAGTCTCGCCTTTTCAAGACAGGCTACTGCCCTTGAACTTTAAAAAAAAAAGGGGAAAAAAGCCTCTCACTTCTAAGTTTTTTAATTGAATTGCCAAGTTCTAATTGGTGTTACATTCTTTAGGAAAAATTTTATGTACGTTGAGGAGAATTGTACCGGACCAGGAGCTTCCACTTCCATGAGAGTTCCATGGGCAAAGACACTTGATGCCTCGCAACTAAGACGCTTCTCTGTGGAATCCTTCATTAACCAAGATGGGTGGATTCCTAGACTCCCTTGA

mRNA sequence

ATGTTAATCACTGAAGTCATTGGGATAATATGCATCCATGCAGAGAATTCTCAAAAAGAAAATGAACCCTTAACTCCTCTGAAATATTTCAAGATCAAAGCTGTGAATCATGCAAGAGCCTTTGACCTTACAGTTCAATCCGTTGTTGTTGTTGACAAGTCGGGAAGAGGCGATTTCCAGACCGTTCAAGCAGCCATTGACTCAGTTCCACCTAATAACAATCAATGGATTAAGATTCAAATAAACCCTGGTGTATACAAAGAAAAGGTGACAATCCCACCAGAAAAACCTTTCATATATCTCGAAGGAGCTGACCGCAGCAATACGATCATCACATTCGATGACCATCAACAAACGGACACTAGTGCTACATTTACTTCACGTCCTCCTAACATTATTGCTAGAGGCATTACTTTTGAGAACTCCTTCAACCTTGAAGAAGCTCCAGAGCTGTTTGTTTGCGATGATGGAACTTACATTACACAAGCAATAGCAGCAAGAATCTATGGCGACAAATCGGCGTTTTTCAACTGTGGATTCAAAGGGTATCAAGACACGTTATGGGATGTGCAAGGTCGCCATTTCTTCCAAAATTGCTACATTGAAGGAGCCATAGACTTCATCTTTGGTAGTGGCCAATCAGTTTATAAGGATTGCTTGATAAATGTTAATGTTGCAAGTCTCCCTCAAGTCCACCAAGGATTCATTACTGCACAAGGTCGAAGATCAGCAGCAGATCCAAGTGGATTTGTATTTAAAGGGTGCACCATAACGGGTAGTGGCAAAGCTTTGTTGGGGAGAGCATATGGTCCTTTTTCCAGAGTTATATTCAAAGATGCTATAATGGGTCCAGTGGTAGCACCAGAGGGATGGGACGCCTGGAACTTCAAGAGCAAAGAGAAAAATTTTATGTACGTTGAGGAGAATTGTACCGGACCAGGAGCTTCCACTTCCATGAGAGTTCCATGGGCAAAGACACTTGATGCCTCGCAACTAAGACGCTTCTCTGTGGAATCCTTCATTAACCAAGATGGGTGGATTCCTAGACTCCCTTGA

Coding sequence (CDS)

ATGTTAATCACTGAAGTCATTGGGATAATATGCATCCATGCAGAGAATTCTCAAAAAGAAAATGAACCCTTAACTCCTCTGAAATATTTCAAGATCAAAGCTGTGAATCATGCAAGAGCCTTTGACCTTACAGTTCAATCCGTTGTTGTTGTTGACAAGTCGGGAAGAGGCGATTTCCAGACCGTTCAAGCAGCCATTGACTCAGTTCCACCTAATAACAATCAATGGATTAAGATTCAAATAAACCCTGGTGTATACAAAGAAAAGGTGACAATCCCACCAGAAAAACCTTTCATATATCTCGAAGGAGCTGACCGCAGCAATACGATCATCACATTCGATGACCATCAACAAACGGACACTAGTGCTACATTTACTTCACGTCCTCCTAACATTATTGCTAGAGGCATTACTTTTGAGAACTCCTTCAACCTTGAAGAAGCTCCAGAGCTGTTTGTTTGCGATGATGGAACTTACATTACACAAGCAATAGCAGCAAGAATCTATGGCGACAAATCGGCGTTTTTCAACTGTGGATTCAAAGGGTATCAAGACACGTTATGGGATGTGCAAGGTCGCCATTTCTTCCAAAATTGCTACATTGAAGGAGCCATAGACTTCATCTTTGGTAGTGGCCAATCAGTTTATAAGGATTGCTTGATAAATGTTAATGTTGCAAGTCTCCCTCAAGTCCACCAAGGATTCATTACTGCACAAGGTCGAAGATCAGCAGCAGATCCAAGTGGATTTGTATTTAAAGGGTGCACCATAACGGGTAGTGGCAAAGCTTTGTTGGGGAGAGCATATGGTCCTTTTTCCAGAGTTATATTCAAAGATGCTATAATGGGTCCAGTGGTAGCACCAGAGGGATGGGACGCCTGGAACTTCAAGAGCAAAGAGAAAAATTTTATGTACGTTGAGGAGAATTGTACCGGACCAGGAGCTTCCACTTCCATGAGAGTTCCATGGGCAAAGACACTTGATGCCTCGCAACTAAGACGCTTCTCTGTGGAATCCTTCATTAACCAAGATGGGTGGATTCCTAGACTCCCTTGA

Protein sequence

MLITEVIGIICIHAENSQKENEPLTPLKYFKIKAVNHARAFDLTVQSVVVVDKSGRGDFQTVQAAIDSVPPNNNQWIKIQINPGVYKEKVTIPPEKPFIYLEGADRSNTIITFDDHQQTDTSATFTSRPPNIIARGITFENSFNLEEAPELFVCDDGTYITQAIAARIYGDKSAFFNCGFKGYQDTLWDVQGRHFFQNCYIEGAIDFIFGSGQSVYKDCLINVNVASLPQVHQGFITAQGRRSAADPSGFVFKGCTITGSGKALLGRAYGPFSRVIFKDAIMGPVVAPEGWDAWNFKSKEKNFMYVEENCTGPGASTSMRVPWAKTLDASQLRRFSVESFINQDGWIPRLP
Homology
BLAST of Pay0006576 vs. ExPASy Swiss-Prot
Match: Q4PSQ5 (Probable pectinesterase 66 OS=Arabidopsis thaliana OX=3702 GN=PME66 PE=2 SV=2)

HSP 1 Score: 306.2 bits (783), Expect = 4.7e-82
Identity = 149/304 (49.01%), Postives = 199/304 (65.46%), Query Frame = 0

Query: 49  VVVDKSGRGDFQTVQAAIDSVPPNNNQWIKIQINPGVYKEKVTIPPEKPFIYLEGADRSN 108
           + VD +G G+F TVQ+AIDS+ P N+ WI++    G+Y+EKVTIP EK FIYL+G     
Sbjct: 35  ITVDLNGGGNFTTVQSAIDSISPPNHNWIRVFTQNGIYREKVTIPKEKGFIYLQGKGIEQ 94

Query: 109 TIITFDDHQQTDTSATFTSRPPNIIARGITFENSFNLEEAPELFVCDDGTYITQAIAARI 168
           T+I +DDHQ TD SATFT+   +I+  GITF+N++N+       V ++   I  A+AAR+
Sbjct: 95  TVIEYDDHQATDISATFTAFADDIVISGITFKNTYNI-------VPNNKREIVPAVAARM 154

Query: 169 YGDKSAFFNCGFKGYQDTLWDVQGRHFFQNCYIEGAIDFIFGSGQSVYKDCLINVNVA-S 228
            GD+    +  F G QDTL+D +GRH+++ C I G IDFIFG GQS++K+C +N+ +   
Sbjct: 155 LGDRYVVTDSSFVGLQDTLFDGKGRHYYKRCIISGGIDFIFGYGQSLFKECTLNMTLGIY 214

Query: 229 LPQVHQGFITAQGRRSAADPSGFVFKGCTITGSGKALLGRAYGPFSRVIFKDAIMGPVVA 288
            P    G ITA  R S +D  GFVF  CT+TG GK LLGRA+G  +RVIF  + +  VV 
Sbjct: 215 APDNPYGTITAHQRPSPSDEGGFVFSDCTVTGVGKTLLGRAWGSNARVIFDRSRLSDVVL 274

Query: 289 PEGWDAWNFKSKEKNFMYVEENCTGPGASTSMRVPWAKTLDASQLRRFSVESFINQDGWI 348
           P GWDAW  K  E++  +VE  CTG GA TS RVPW K L  S++  F+  SFI+QDGWI
Sbjct: 275 PIGWDAWRAKGNERDLTFVEAGCTGAGADTSQRVPWLKKLSLSEVDGFASVSFIDQDGWI 331

Query: 349 PRLP 352
            R P
Sbjct: 335 SRFP 331

BLAST of Pay0006576 vs. ExPASy Swiss-Prot
Match: Q3E9D3 (Probable pectinesterase 55 OS=Arabidopsis thaliana OX=3702 GN=PME55 PE=2 SV=1)

HSP 1 Score: 298.1 bits (762), Expect = 1.3e-79
Identity = 144/303 (47.52%), Postives = 192/303 (63.37%), Query Frame = 0

Query: 49  VVVDKSGRGDFQTVQAAIDSVPPNNNQWIKIQINPGVYKEKVTIPPEKPFIYLEGADRSN 108
           V+VD+SG G+F T+Q AIDSVP NN  W  I +  G+Y+EK+TIP +KPFI + GA + +
Sbjct: 29  VIVDQSGHGNFTTIQKAIDSVPINNTHWFFINVKAGLYREKITIPQKKPFIVIVGAGKRS 88

Query: 109 TIITFDDHQQTDTSATFTSRPPNIIARGITFENSFNLEEAPELFVCDDGTYITQAIAARI 168
           T + +DDH     S TF +   N + + ITF NS+N     ++    +      A+AA I
Sbjct: 89  TRVEWDDHASLAQSPTFATLADNTVVKKITFANSYNFPSNGKI----NKNPRVPAVAAFI 148

Query: 169 YGDKSAFFNCGFKGYQDTLWDVQGRHFFQNCYIEGAIDFIFGSGQSVYKDCLINVNVASL 228
            GDKSAF++ GF G QDTLWD  GRH+F  C I+GA+DFI GSGQS+Y+ C+I V    L
Sbjct: 149 GGDKSAFYSVGFAGIQDTLWDSDGRHYFHRCTIQGAVDFILGSGQSIYQSCVIQVLGGQL 208

Query: 229 PQVHQGFITAQGRRSAADPSGFVFKGCTITGSGKALLGRAYGPFSRVIFKDAIMGPVVAP 288
                G+ITAQGR +A D +GFVF  C + G GKA LGRA+ P+SRVIF ++ +  VV P
Sbjct: 209 GPGVTGYITAQGRTNANDANGFVFINCLVHGFGKAYLGRAWRPYSRVIFYNSNLTDVVDP 268

Query: 289 EGWDAWNFKSKEKNFMYVEENCTGPGASTSMRVPWAKTLDASQLRRFSVESFINQDGWIP 348
            GW  WN++  EK   Y E  C G G++TS R  W K L AS ++  +  SFIN+ GW+ 
Sbjct: 269 LGWWEWNYQGYEKQLTYAEHGCFGSGSNTSRRAKWVKKLSASAVQHLADLSFINRGGWVE 327

Query: 349 RLP 352
            LP
Sbjct: 329 DLP 327

BLAST of Pay0006576 vs. ExPASy Swiss-Prot
Match: Q4PSN0 (Probable pectinesterase 29 OS=Arabidopsis thaliana OX=3702 GN=PME29 PE=2 SV=1)

HSP 1 Score: 297.7 bits (761), Expect = 1.7e-79
Identity = 143/306 (46.73%), Postives = 190/306 (62.09%), Query Frame = 0

Query: 46  QSVVVVDKSGRGDFQTVQAAIDSVPPNNNQWIKIQINPGVYKEKVTIPPEKPFIYLEGAD 105
           Q  V VD+SG G+F T+Q AIDSVP NN  W  I +  G+Y+EK+ IP EKPFI L GA 
Sbjct: 31  QQQVFVDQSGHGNFTTIQKAIDSVPINNRHWFFINVKAGLYREKIKIPYEKPFIVLVGAG 90

Query: 106 RSNTIITFDDHQQTDTSATFTSRPPNIIARGITFENSFNLEEAPELFVCDDGTYITQAIA 165
           +  T + +DDH     S TF++   N + + ITF NS+N     ++    +    T A+A
Sbjct: 91  KRLTRVEWDDHYSVAQSPTFSTLADNTVVKSITFANSYNFPSKGKM----NKNPRTPAVA 150

Query: 166 ARIYGDKSAFFNCGFKGYQDTLWDVQGRHFFQNCYIEGAIDFIFGSGQSVYKDCLINVNV 225
           A I GDKSAF++ GF G QDTLWD  GRH+F  C I+GA+DFIFG+GQS+Y+ C+I V  
Sbjct: 151 ALIGGDKSAFYSVGFAGIQDTLWDFDGRHYFHRCTIQGAVDFIFGTGQSIYQSCVIQVLG 210

Query: 226 ASLPQVHQGFITAQGRRSAADPSGFVFKGCTITGSGKALLGRAYGPFSRVIFKDAIMGPV 285
             L     G+ITAQGR +  D +GF+F  C + G+G A LGR +  +SRVIF ++ +  V
Sbjct: 211 GQLEPGLAGYITAQGRTNPYDANGFIFINCLVYGTGMAFLGRPWRGYSRVIFYNSNLTDV 270

Query: 286 VAPEGWDAWNFKSKEKNFMYVEENCTGPGASTSMRVPWAKTLDASQLRRFSVESFINQDG 345
           V PEGWDAWNF   E   ++ E  C G GA+   RV W K L  S ++  +  SFIN+ G
Sbjct: 271 VVPEGWDAWNFVGHENQLVFAEHGCFGSGANIGRRVKWVKKLSESAIQNLADLSFINRGG 330

Query: 346 WIPRLP 352
           W+  LP
Sbjct: 331 WVEDLP 332

BLAST of Pay0006576 vs. ExPASy Swiss-Prot
Match: O64479 (Putative pectinesterase 10 OS=Arabidopsis thaliana OX=3702 GN=PME10 PE=2 SV=1)

HSP 1 Score: 292.7 bits (748), Expect = 5.4e-78
Identity = 142/308 (46.10%), Postives = 192/308 (62.34%), Query Frame = 0

Query: 45  VQSVVVVDKSGRGDFQTVQAAIDSVPPNNNQWIKIQINPGVYKEKVTIPPEKPFIYLEGA 104
           +   ++V+ +    F+TVQ+AIDS+P  N  WI+I I+ G+Y EKVTIP  K +IY++G 
Sbjct: 37  IAKTIIVNPNDARYFKTVQSAIDSIPLQNQDWIRILISNGIYSEKVTIPRGKGYIYMQGG 96

Query: 105 DRSNTIITFDDHQQTDTSATFTSRPPNIIARGITFENSFNLEEAPELFVCDDGTYITQAI 164
               TII + DHQ T+TSATFTS P NII  GITF+N +N        +    +    A+
Sbjct: 97  GIEKTIIAYGDHQLTNTSATFTSYPSNIIITGITFKNKYN--------IASSSSPTKPAV 156

Query: 165 AARIYGDKSAFFNCGFKGYQDTLWDVQGRHFFQNCYIEGAIDFIFGSGQSVYKDCLINVN 224
           AA + GDK A  +  F G+QDTL+D  GRH+++ C I G IDFIFG  QS+++ C + + 
Sbjct: 157 AAMMLGDKYAIIDSSFDGFQDTLYDDYGRHYYKRCVISGGIDFIFGGAQSIFEGCTLKLR 216

Query: 225 VASLPQVH-QGFITAQGRRSAADPSGFVFKGCTITGSGKALLGRAYGPFSRVIFKDAIMG 284
           V   P     G ITAQGR S  D  GFVFK CT+ GSGKALLGRA+  +SRVIF  ++  
Sbjct: 217 VGIYPPNEVYGTITAQGRDSPTDKGGFVFKDCTVMGSGKALLGRAWKSYSRVIFYRSMFS 276

Query: 285 PVVAPEGWDAWNFKSKEKNFMYVEENCTGPGASTSMRVPWAKTLDASQLRRFSVESFINQ 344
             + P GWDAW  K +E +  +VE  CTG GA TS RVPW        + +F+  +FI++
Sbjct: 277 DNILPIGWDAWKAKGQEGHITFVEFGCTGVGADTSKRVPWLTKASEKDVLQFTNLTFIDE 336

Query: 345 DGWIPRLP 352
           +GW+ RLP
Sbjct: 337 EGWLSRLP 336

BLAST of Pay0006576 vs. ExPASy Swiss-Prot
Match: O04953 (Putative pectinesterase 52 OS=Arabidopsis thaliana OX=3702 GN=PME52 PE=2 SV=2)

HSP 1 Score: 251.1 bits (640), Expect = 1.8e-65
Identity = 125/252 (49.60%), Postives = 164/252 (65.08%), Query Frame = 0

Query: 103 GADRSNTIITFDDHQQTDTSATFTSRPPNIIARGITFENSFNLEEAPELFVCDDGTY-IT 162
           G  +  T IT++ H  TD S+TFTS P +I+ R ++  N++N      L   +  ++ I 
Sbjct: 42  GEGQRVTTITYNGHAATDVSSTFTSYPSHIVVRNLSIMNTYN--RLTSLTKANGMSWDIK 101

Query: 163 QAIAARIYGDKSAFFNCGFKGYQDTLWDVQGRHFFQNCYIEGAIDFIFGSGQSVYKDCLI 222
            A+A  +YGDKSAF+NC F G QDT+WD  GRH F+NCYIEGAIDFIFGSGQSVY+DC I
Sbjct: 102 PAVAISVYGDKSAFYNCDFLGLQDTVWDNLGRHHFKNCYIEGAIDFIFGSGQSVYEDCHI 161

Query: 223 NVNVASL-PQVHQGFITAQGRRSAADPSGFVFKGCTITGSGKALLGRAYGPFSRVIFKDA 282
           N    +L  +V  G+ITAQGR S +DPSGFVF   +++GS    LGRAYGPFSRVIF   
Sbjct: 162 NATAGALASKVSFGYITAQGRSSDSDPSGFVFLRGSVSGSTSVYLGRAYGPFSRVIFIQT 221

Query: 283 IMGPVVAPEGWDAWNFKSKEKNFMYVEENCTGPGASTSMRVPWAKTLDASQLR-RFSVES 342
            +  VV PEGW +W++   E +F Y E  C G G+  S RVPW   L +   + +FS+ +
Sbjct: 222 DLSSVVHPEGWYSWHYGGYEMSFTYAEVECKGAGSDMSRRVPWIDKLHSFYTKQQFSISN 281

Query: 343 FINQDGWIPRLP 352
           FI+QD WI  +P
Sbjct: 282 FIDQDQWISNIP 291

BLAST of Pay0006576 vs. ExPASy TrEMBL
Match: A0A5A7U1G9 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold385G001480 PE=4 SV=1)

HSP 1 Score: 665.2 bits (1715), Expect = 1.5e-187
Identity = 318/322 (98.76%), Postives = 319/322 (99.07%), Query Frame = 0

Query: 30  FKIKAVNHARAFDLTVQSVVVVDKSGRGDFQTVQAAIDSVPPNNNQWIKIQINPGVYKEK 89
           F  +AVNHARAFDLTVQSVVVVDKSGRGDFQTVQAAIDSVPPNNNQWIKIQINPGVYKEK
Sbjct: 19  FGFEAVNHARAFDLTVQSVVVVDKSGRGDFQTVQAAIDSVPPNNNQWIKIQINPGVYKEK 78

Query: 90  VTIPPEKPFIYLEGADRSNTIITFDDHQQTDTSATFTSRPPNIIARGITFENSFNLEEAP 149
           VTIPPEKPFIYLEGADRSNTIITFDDHQQTDTSATFTSRPPNIIARGITFENSFNLEEAP
Sbjct: 79  VTIPPEKPFIYLEGADRSNTIITFDDHQQTDTSATFTSRPPNIIARGITFENSFNLEEAP 138

Query: 150 ELFVCDDGTYITQAIAARIYGDKSAFFNCGFKGYQDTLWDVQGRHFFQNCYIEGAIDFIF 209
           ELFVCDDGTYITQAIAARIYGDKSAFFNCGFKGYQDTLWDVQGRHFFQNCYIEGAIDFIF
Sbjct: 139 ELFVCDDGTYITQAIAARIYGDKSAFFNCGFKGYQDTLWDVQGRHFFQNCYIEGAIDFIF 198

Query: 210 GSGQSVYKDCLINVNVASLPQVHQGFITAQGRRSAADPSGFVFKGCTITGSGKALLGRAY 269
           GSGQSVYKDCLINVNVASLPQVHQGFITAQGRRSAADPSGFVFKGCTITGSGKALLGRAY
Sbjct: 199 GSGQSVYKDCLINVNVASLPQVHQGFITAQGRRSAADPSGFVFKGCTITGSGKALLGRAY 258

Query: 270 GPFSRVIFKDAIMGPVVAPEGWDAWNFKSKEKNFMYVEENCTGPGASTSMRVPWAKTLDA 329
           GPFSRVIFKDAIMGPVVAPEGWDAWNFKSKEKNFMYVEENCTGPGASTS RVPWAKTLDA
Sbjct: 259 GPFSRVIFKDAIMGPVVAPEGWDAWNFKSKEKNFMYVEENCTGPGASTSTRVPWAKTLDA 318

Query: 330 SQLRRFSVESFINQDGWIPRLP 352
           SQLRRFSVESFINQDGWIPRLP
Sbjct: 319 SQLRRFSVESFINQDGWIPRLP 340

BLAST of Pay0006576 vs. ExPASy TrEMBL
Match: A0A5D3BBR9 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold546G001020 PE=4 SV=1)

HSP 1 Score: 663.3 bits (1710), Expect = 5.6e-187
Identity = 317/322 (98.45%), Postives = 318/322 (98.76%), Query Frame = 0

Query: 30  FKIKAVNHARAFDLTVQSVVVVDKSGRGDFQTVQAAIDSVPPNNNQWIKIQINPGVYKEK 89
           F  +AVNHARAFDLTVQSVVVVDKSGRGDFQTVQAAIDSVPPNNNQWIKIQINPGVYKEK
Sbjct: 19  FGFEAVNHARAFDLTVQSVVVVDKSGRGDFQTVQAAIDSVPPNNNQWIKIQINPGVYKEK 78

Query: 90  VTIPPEKPFIYLEGADRSNTIITFDDHQQTDTSATFTSRPPNIIARGITFENSFNLEEAP 149
           VTIPPEKPFIYLEGADRSNTIITFDDHQQTDTSATFTSRPPNIIARGITFENSFNLEEAP
Sbjct: 79  VTIPPEKPFIYLEGADRSNTIITFDDHQQTDTSATFTSRPPNIIARGITFENSFNLEEAP 138

Query: 150 ELFVCDDGTYITQAIAARIYGDKSAFFNCGFKGYQDTLWDVQGRHFFQNCYIEGAIDFIF 209
           ELFVCDDGTYITQAIAARIYGDKSAFFNCGFKGYQDTLWDVQGRHFFQNCYIEGAIDFIF
Sbjct: 139 ELFVCDDGTYITQAIAARIYGDKSAFFNCGFKGYQDTLWDVQGRHFFQNCYIEGAIDFIF 198

Query: 210 GSGQSVYKDCLINVNVASLPQVHQGFITAQGRRSAADPSGFVFKGCTITGSGKALLGRAY 269
           GSGQSVYKDCLINVNVASLPQVHQGFITAQGRRSAADPSGFVFKGC ITGSGKALLGRAY
Sbjct: 199 GSGQSVYKDCLINVNVASLPQVHQGFITAQGRRSAADPSGFVFKGCAITGSGKALLGRAY 258

Query: 270 GPFSRVIFKDAIMGPVVAPEGWDAWNFKSKEKNFMYVEENCTGPGASTSMRVPWAKTLDA 329
           GPFSRVIFKDAIMGPVVAPEGWDAWNFKSKEKNFMYVEENCTGPGASTS RVPWAKTLDA
Sbjct: 259 GPFSRVIFKDAIMGPVVAPEGWDAWNFKSKEKNFMYVEENCTGPGASTSTRVPWAKTLDA 318

Query: 330 SQLRRFSVESFINQDGWIPRLP 352
           SQLRRFSVESFINQDGWIPRLP
Sbjct: 319 SQLRRFSVESFINQDGWIPRLP 340

BLAST of Pay0006576 vs. ExPASy TrEMBL
Match: A0A1S4DZS3 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103499379 PE=4 SV=1)

HSP 1 Score: 646.4 bits (1666), Expect = 7.1e-182
Identity = 318/352 (90.34%), Postives = 320/352 (90.91%), Query Frame = 0

Query: 30  FKIKAVNHARAFDLTVQSVVVVDKSGRGDFQTVQAAIDSVPPNNNQWIKIQINPGVY--- 89
           F  +AVNHARAFDLTVQSVVVVDKSGRGDFQTVQAAIDSVPPNNNQWIKIQINPGVY   
Sbjct: 19  FGFEAVNHARAFDLTVQSVVVVDKSGRGDFQTVQAAIDSVPPNNNQWIKIQINPGVYNWQ 78

Query: 90  ---KEKVTIPPEKPFIYLEGADRSNTIITFDDHQQTDTSATFTSRPPNIIARGITFE--- 149
              +EKVTIPPEKPFIYLEGADRSNTIITFDDHQQTDTSATFTSRPPNIIARGITFE   
Sbjct: 79  TFCREKVTIPPEKPFIYLEGADRSNTIITFDDHQQTDTSATFTSRPPNIIARGITFEVLW 138

Query: 150 ---------------------NSFNLEEAPELFVCDDGTYITQAIAARIYGDKSAFFNCG 209
                                NSFNLEEAPELFVCDDGTYITQAIAARIYGDKSAFFNCG
Sbjct: 139 LLKTEFIFIASFEILKPKCTQNSFNLEEAPELFVCDDGTYITQAIAARIYGDKSAFFNCG 198

Query: 210 FKGYQDTLWDVQGRHFFQNCYIEGAIDFIFGSGQSVYKDCLINVNVASLPQVHQGFITAQ 269
           FKGYQDTLWDVQGRHFFQNCYIEGAIDFIFGSGQSVYKDCLINVNVASLPQVHQGFITAQ
Sbjct: 199 FKGYQDTLWDVQGRHFFQNCYIEGAIDFIFGSGQSVYKDCLINVNVASLPQVHQGFITAQ 258

Query: 270 GRRSAADPSGFVFKGCTITGSGKALLGRAYGPFSRVIFKDAIMGPVVAPEGWDAWNFKSK 329
           GRRSAADPSGFVFKGCTITGSGKALLGRAYGPFSRVIFKDAIMGPVVAPEGWDAWNFKSK
Sbjct: 259 GRRSAADPSGFVFKGCTITGSGKALLGRAYGPFSRVIFKDAIMGPVVAPEGWDAWNFKSK 318

Query: 330 EKNFMYVEENCTGPGASTSMRVPWAKTLDASQLRRFSVESFINQDGWIPRLP 352
           EKNFMYVEENCTGPGASTSMRVPWAKTLDASQLRRFSVESFINQDGWIPRLP
Sbjct: 319 EKNFMYVEENCTGPGASTSMRVPWAKTLDASQLRRFSVESFINQDGWIPRLP 370

BLAST of Pay0006576 vs. ExPASy TrEMBL
Match: A0A0A0LH00 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_2G034610 PE=4 SV=1)

HSP 1 Score: 596.3 bits (1536), Expect = 8.4e-167
Identity = 284/319 (89.03%), Postives = 297/319 (93.10%), Query Frame = 0

Query: 30  FKIKAVNHARAFDLTVQSVVVVDKSGRGDFQTVQAAIDSVPPNNNQWIKIQINPGVYKEK 89
           F  +AVN AR F LT+QSVV+VDKSG G+FQTVQAAIDSVPPNNN WIKIQINPGVYKEK
Sbjct: 19  FGFEAVNQARDFVLTIQSVVIVDKSGNGNFQTVQAAIDSVPPNNNHWIKIQINPGVYKEK 78

Query: 90  VTIPPEKPFIYLEGADRSNTIITFDDHQQTDTSATFTSRPPNIIARGITFENSFNLEEAP 149
           VTIP EKPFIYLEGAD SNT+ITFDDHQQTDTSATFTSRPPNII RGITFENSFNL EAP
Sbjct: 79  VTIPLEKPFIYLEGADSSNTVITFDDHQQTDTSATFTSRPPNIIVRGITFENSFNLREAP 138

Query: 150 ELFVCDDGTYITQAIAARIYGDKSAFFNCGFKGYQDTLWDVQGRHFFQNCYIEGAIDFIF 209
           ELF CDDGTYITQAIAARIYGDKSAFFNCGFKGYQDTLWDVQGRHFF +CYIEGAIDFIF
Sbjct: 139 ELFSCDDGTYITQAIAARIYGDKSAFFNCGFKGYQDTLWDVQGRHFFSHCYIEGAIDFIF 198

Query: 210 GSGQSVYKDCLINVNVASLPQVHQGFITAQGRRSAADPSGFVFKGCTITGSGKALLGRAY 269
           GSGQSVY+DC+INVNVASLPQV+QG+ITAQ R+SAADPSGFVFK CTI GSGKALLGRAY
Sbjct: 199 GSGQSVYEDCMINVNVASLPQVYQGYITAQSRQSAADPSGFVFKECTIKGSGKALLGRAY 258

Query: 270 GPFSRVIFKDAIMGPVVAPEGWDAWNFKSKEKNFMYVEENCTGPGASTSMRVPWAKTLDA 329
           GPFSRVIFKDAIMG VVAPEGW AW+FK KE+NFMYVEENCTGPGASTSMRVPWAKTLDA
Sbjct: 259 GPFSRVIFKDAIMGSVVAPEGWYAWHFKGKEENFMYVEENCTGPGASTSMRVPWAKTLDA 318

Query: 330 SQLRRFSVESFINQDGWIP 349
           S L  FSVESFINQDGWIP
Sbjct: 319 SHLTGFSVESFINQDGWIP 337

BLAST of Pay0006576 vs. ExPASy TrEMBL
Match: A0A6J1G5A0 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111450826 PE=4 SV=1)

HSP 1 Score: 482.3 bits (1240), Expect = 1.8e-132
Identity = 225/315 (71.43%), Postives = 264/315 (83.81%), Query Frame = 0

Query: 34  AVNHARAFDLTVQSVVVVDKSGRGDFQTVQAAIDSVPPNNNQWIKIQINPGVYKEKVTIP 93
           A+N  RA DL +QSV+VVDKSG+G+F+T+QAAIDSVP NNN+W+KIQ+NPG Y+E VTIP
Sbjct: 5   AMNFTRALDLKIQSVIVVDKSGKGNFKTIQAAIDSVPLNNNKWVKIQVNPGEYREMVTIP 64

Query: 94  PEKPFIYLEGADRSNTIITFDDHQQTDTSATFTSRPPNIIARGITFENSFNLEEAPELFV 153
             K FIYL+G  RS T+ITF+ HQQT+TSATF S   NIIARGITFENSFNL  AP+LF 
Sbjct: 65  STKSFIYLQGGSRSTTVITFNGHQQTNTSATFNSMADNIIARGITFENSFNLANAPQLFD 124

Query: 154 CDDGTYITQAIAARIYGDKSAFFNCGFKGYQDTLWDVQGRHFFQNCYIEGAIDFIFGSGQ 213
              GT+ TQA+AARIYGDKSAF +CGFKG+QDTLWDV+GRH+F++CYIEGAIDFIFG+GQ
Sbjct: 125 KITGTFFTQALAARIYGDKSAFISCGFKGFQDTLWDVKGRHYFKSCYIEGAIDFIFGNGQ 184

Query: 214 SVYKDCLINVNVASLPQVHQGFITAQGRRSAADPSGFVFKGCTITGSGKALLGRAYGPFS 273
           S+Y+ CLI VNV  LP+V+QG+ITAQGR+SA DPSGFVF+ CTITGSGK  LGRAYGPFS
Sbjct: 185 SIYEGCLIYVNVGLLPEVNQGYITAQGRQSANDPSGFVFQQCTITGSGKGFLGRAYGPFS 244

Query: 274 RVIFKDAIMGPVVAPEGWDAWNFKSKEKNFMYVEENCTGPGASTSMRVPWAKTLDASQLR 333
           RVIFKD  MG VVAPEGWDAW+FK +E NF+Y+E N  G G+S S RVPWA T   SQL 
Sbjct: 245 RVIFKDTNMGKVVAPEGWDAWHFKGQETNFVYIESNSRGIGSSPSKRVPWAMTQGNSQLS 304

Query: 334 RFSVESFINQDGWIP 349
            +SV+SFINQDGWIP
Sbjct: 305 GYSVKSFINQDGWIP 319

BLAST of Pay0006576 vs. NCBI nr
Match: KAA0048077.1 (putative pectinesterase 66 [Cucumis melo var. makuwa])

HSP 1 Score: 665.2 bits (1715), Expect = 3.0e-187
Identity = 318/322 (98.76%), Postives = 319/322 (99.07%), Query Frame = 0

Query: 30  FKIKAVNHARAFDLTVQSVVVVDKSGRGDFQTVQAAIDSVPPNNNQWIKIQINPGVYKEK 89
           F  +AVNHARAFDLTVQSVVVVDKSGRGDFQTVQAAIDSVPPNNNQWIKIQINPGVYKEK
Sbjct: 19  FGFEAVNHARAFDLTVQSVVVVDKSGRGDFQTVQAAIDSVPPNNNQWIKIQINPGVYKEK 78

Query: 90  VTIPPEKPFIYLEGADRSNTIITFDDHQQTDTSATFTSRPPNIIARGITFENSFNLEEAP 149
           VTIPPEKPFIYLEGADRSNTIITFDDHQQTDTSATFTSRPPNIIARGITFENSFNLEEAP
Sbjct: 79  VTIPPEKPFIYLEGADRSNTIITFDDHQQTDTSATFTSRPPNIIARGITFENSFNLEEAP 138

Query: 150 ELFVCDDGTYITQAIAARIYGDKSAFFNCGFKGYQDTLWDVQGRHFFQNCYIEGAIDFIF 209
           ELFVCDDGTYITQAIAARIYGDKSAFFNCGFKGYQDTLWDVQGRHFFQNCYIEGAIDFIF
Sbjct: 139 ELFVCDDGTYITQAIAARIYGDKSAFFNCGFKGYQDTLWDVQGRHFFQNCYIEGAIDFIF 198

Query: 210 GSGQSVYKDCLINVNVASLPQVHQGFITAQGRRSAADPSGFVFKGCTITGSGKALLGRAY 269
           GSGQSVYKDCLINVNVASLPQVHQGFITAQGRRSAADPSGFVFKGCTITGSGKALLGRAY
Sbjct: 199 GSGQSVYKDCLINVNVASLPQVHQGFITAQGRRSAADPSGFVFKGCTITGSGKALLGRAY 258

Query: 270 GPFSRVIFKDAIMGPVVAPEGWDAWNFKSKEKNFMYVEENCTGPGASTSMRVPWAKTLDA 329
           GPFSRVIFKDAIMGPVVAPEGWDAWNFKSKEKNFMYVEENCTGPGASTS RVPWAKTLDA
Sbjct: 259 GPFSRVIFKDAIMGPVVAPEGWDAWNFKSKEKNFMYVEENCTGPGASTSTRVPWAKTLDA 318

Query: 330 SQLRRFSVESFINQDGWIPRLP 352
           SQLRRFSVESFINQDGWIPRLP
Sbjct: 319 SQLRRFSVESFINQDGWIPRLP 340

BLAST of Pay0006576 vs. NCBI nr
Match: TYJ96459.1 (putative pectinesterase 66 [Cucumis melo var. makuwa])

HSP 1 Score: 663.3 bits (1710), Expect = 1.2e-186
Identity = 317/322 (98.45%), Postives = 318/322 (98.76%), Query Frame = 0

Query: 30  FKIKAVNHARAFDLTVQSVVVVDKSGRGDFQTVQAAIDSVPPNNNQWIKIQINPGVYKEK 89
           F  +AVNHARAFDLTVQSVVVVDKSGRGDFQTVQAAIDSVPPNNNQWIKIQINPGVYKEK
Sbjct: 19  FGFEAVNHARAFDLTVQSVVVVDKSGRGDFQTVQAAIDSVPPNNNQWIKIQINPGVYKEK 78

Query: 90  VTIPPEKPFIYLEGADRSNTIITFDDHQQTDTSATFTSRPPNIIARGITFENSFNLEEAP 149
           VTIPPEKPFIYLEGADRSNTIITFDDHQQTDTSATFTSRPPNIIARGITFENSFNLEEAP
Sbjct: 79  VTIPPEKPFIYLEGADRSNTIITFDDHQQTDTSATFTSRPPNIIARGITFENSFNLEEAP 138

Query: 150 ELFVCDDGTYITQAIAARIYGDKSAFFNCGFKGYQDTLWDVQGRHFFQNCYIEGAIDFIF 209
           ELFVCDDGTYITQAIAARIYGDKSAFFNCGFKGYQDTLWDVQGRHFFQNCYIEGAIDFIF
Sbjct: 139 ELFVCDDGTYITQAIAARIYGDKSAFFNCGFKGYQDTLWDVQGRHFFQNCYIEGAIDFIF 198

Query: 210 GSGQSVYKDCLINVNVASLPQVHQGFITAQGRRSAADPSGFVFKGCTITGSGKALLGRAY 269
           GSGQSVYKDCLINVNVASLPQVHQGFITAQGRRSAADPSGFVFKGC ITGSGKALLGRAY
Sbjct: 199 GSGQSVYKDCLINVNVASLPQVHQGFITAQGRRSAADPSGFVFKGCAITGSGKALLGRAY 258

Query: 270 GPFSRVIFKDAIMGPVVAPEGWDAWNFKSKEKNFMYVEENCTGPGASTSMRVPWAKTLDA 329
           GPFSRVIFKDAIMGPVVAPEGWDAWNFKSKEKNFMYVEENCTGPGASTS RVPWAKTLDA
Sbjct: 259 GPFSRVIFKDAIMGPVVAPEGWDAWNFKSKEKNFMYVEENCTGPGASTSTRVPWAKTLDA 318

Query: 330 SQLRRFSVESFINQDGWIPRLP 352
           SQLRRFSVESFINQDGWIPRLP
Sbjct: 319 SQLRRFSVESFINQDGWIPRLP 340

BLAST of Pay0006576 vs. NCBI nr
Match: XP_016901459.1 (PREDICTED: probable pectinesterase 66 [Cucumis melo])

HSP 1 Score: 646.4 bits (1666), Expect = 1.5e-181
Identity = 318/352 (90.34%), Postives = 320/352 (90.91%), Query Frame = 0

Query: 30  FKIKAVNHARAFDLTVQSVVVVDKSGRGDFQTVQAAIDSVPPNNNQWIKIQINPGVY--- 89
           F  +AVNHARAFDLTVQSVVVVDKSGRGDFQTVQAAIDSVPPNNNQWIKIQINPGVY   
Sbjct: 19  FGFEAVNHARAFDLTVQSVVVVDKSGRGDFQTVQAAIDSVPPNNNQWIKIQINPGVYNWQ 78

Query: 90  ---KEKVTIPPEKPFIYLEGADRSNTIITFDDHQQTDTSATFTSRPPNIIARGITFE--- 149
              +EKVTIPPEKPFIYLEGADRSNTIITFDDHQQTDTSATFTSRPPNIIARGITFE   
Sbjct: 79  TFCREKVTIPPEKPFIYLEGADRSNTIITFDDHQQTDTSATFTSRPPNIIARGITFEVLW 138

Query: 150 ---------------------NSFNLEEAPELFVCDDGTYITQAIAARIYGDKSAFFNCG 209
                                NSFNLEEAPELFVCDDGTYITQAIAARIYGDKSAFFNCG
Sbjct: 139 LLKTEFIFIASFEILKPKCTQNSFNLEEAPELFVCDDGTYITQAIAARIYGDKSAFFNCG 198

Query: 210 FKGYQDTLWDVQGRHFFQNCYIEGAIDFIFGSGQSVYKDCLINVNVASLPQVHQGFITAQ 269
           FKGYQDTLWDVQGRHFFQNCYIEGAIDFIFGSGQSVYKDCLINVNVASLPQVHQGFITAQ
Sbjct: 199 FKGYQDTLWDVQGRHFFQNCYIEGAIDFIFGSGQSVYKDCLINVNVASLPQVHQGFITAQ 258

Query: 270 GRRSAADPSGFVFKGCTITGSGKALLGRAYGPFSRVIFKDAIMGPVVAPEGWDAWNFKSK 329
           GRRSAADPSGFVFKGCTITGSGKALLGRAYGPFSRVIFKDAIMGPVVAPEGWDAWNFKSK
Sbjct: 259 GRRSAADPSGFVFKGCTITGSGKALLGRAYGPFSRVIFKDAIMGPVVAPEGWDAWNFKSK 318

Query: 330 EKNFMYVEENCTGPGASTSMRVPWAKTLDASQLRRFSVESFINQDGWIPRLP 352
           EKNFMYVEENCTGPGASTSMRVPWAKTLDASQLRRFSVESFINQDGWIPRLP
Sbjct: 319 EKNFMYVEENCTGPGASTSMRVPWAKTLDASQLRRFSVESFINQDGWIPRLP 370

BLAST of Pay0006576 vs. NCBI nr
Match: KGN61008.1 (hypothetical protein Csa_021278 [Cucumis sativus])

HSP 1 Score: 596.3 bits (1536), Expect = 1.7e-166
Identity = 284/319 (89.03%), Postives = 297/319 (93.10%), Query Frame = 0

Query: 30  FKIKAVNHARAFDLTVQSVVVVDKSGRGDFQTVQAAIDSVPPNNNQWIKIQINPGVYKEK 89
           F  +AVN AR F LT+QSVV+VDKSG G+FQTVQAAIDSVPPNNN WIKIQINPGVYKEK
Sbjct: 19  FGFEAVNQARDFVLTIQSVVIVDKSGNGNFQTVQAAIDSVPPNNNHWIKIQINPGVYKEK 78

Query: 90  VTIPPEKPFIYLEGADRSNTIITFDDHQQTDTSATFTSRPPNIIARGITFENSFNLEEAP 149
           VTIP EKPFIYLEGAD SNT+ITFDDHQQTDTSATFTSRPPNII RGITFENSFNL EAP
Sbjct: 79  VTIPLEKPFIYLEGADSSNTVITFDDHQQTDTSATFTSRPPNIIVRGITFENSFNLREAP 138

Query: 150 ELFVCDDGTYITQAIAARIYGDKSAFFNCGFKGYQDTLWDVQGRHFFQNCYIEGAIDFIF 209
           ELF CDDGTYITQAIAARIYGDKSAFFNCGFKGYQDTLWDVQGRHFF +CYIEGAIDFIF
Sbjct: 139 ELFSCDDGTYITQAIAARIYGDKSAFFNCGFKGYQDTLWDVQGRHFFSHCYIEGAIDFIF 198

Query: 210 GSGQSVYKDCLINVNVASLPQVHQGFITAQGRRSAADPSGFVFKGCTITGSGKALLGRAY 269
           GSGQSVY+DC+INVNVASLPQV+QG+ITAQ R+SAADPSGFVFK CTI GSGKALLGRAY
Sbjct: 199 GSGQSVYEDCMINVNVASLPQVYQGYITAQSRQSAADPSGFVFKECTIKGSGKALLGRAY 258

Query: 270 GPFSRVIFKDAIMGPVVAPEGWDAWNFKSKEKNFMYVEENCTGPGASTSMRVPWAKTLDA 329
           GPFSRVIFKDAIMG VVAPEGW AW+FK KE+NFMYVEENCTGPGASTSMRVPWAKTLDA
Sbjct: 259 GPFSRVIFKDAIMGSVVAPEGWYAWHFKGKEENFMYVEENCTGPGASTSMRVPWAKTLDA 318

Query: 330 SQLRRFSVESFINQDGWIP 349
           S L  FSVESFINQDGWIP
Sbjct: 319 SHLTGFSVESFINQDGWIP 337

BLAST of Pay0006576 vs. NCBI nr
Match: XP_031736683.1 (probable pectinesterase 66 [Cucumis sativus])

HSP 1 Score: 583.6 bits (1503), Expect = 1.2e-162
Identity = 284/341 (83.28%), Postives = 297/341 (87.10%), Query Frame = 0

Query: 30  FKIKAVNHARAFDLTVQSVVVVDKSGRGDFQTVQAAIDSVPPNNNQWIKIQINPGVYKEK 89
           F  +AVN AR F LT+QSVV+VDKSG G+FQTVQAAIDSVPPNNN WIKIQINPGVYKEK
Sbjct: 19  FGFEAVNQARDFVLTIQSVVIVDKSGNGNFQTVQAAIDSVPPNNNHWIKIQINPGVYKEK 78

Query: 90  VTIPPEKPFIYLEGADRSNTIITFDDHQQTDTSATFTSRPPNIIARGITFE--------- 149
           VTIP EKPFIYLEGAD SNT+ITFDDHQQTDTSATFTSRPPNII RGITFE         
Sbjct: 79  VTIPLEKPFIYLEGADSSNTVITFDDHQQTDTSATFTSRPPNIIVRGITFEVLWLLKTDF 138

Query: 150 -------------NSFNLEEAPELFVCDDGTYITQAIAARIYGDKSAFFNCGFKGYQDTL 209
                        NSFNL EAPELF CDDGTYITQAIAARIYGDKSAFFNCGFKGYQDTL
Sbjct: 139 IFIALFEILKLCKNSFNLREAPELFSCDDGTYITQAIAARIYGDKSAFFNCGFKGYQDTL 198

Query: 210 WDVQGRHFFQNCYIEGAIDFIFGSGQSVYKDCLINVNVASLPQVHQGFITAQGRRSAADP 269
           WDVQGRHFF +CYIEGAIDFIFGSGQSVY+DC+INVNVASLPQV+QG+ITAQ R+SAADP
Sbjct: 199 WDVQGRHFFSHCYIEGAIDFIFGSGQSVYEDCMINVNVASLPQVYQGYITAQSRQSAADP 258

Query: 270 SGFVFKGCTITGSGKALLGRAYGPFSRVIFKDAIMGPVVAPEGWDAWNFKSKEKNFMYVE 329
           SGFVFK CTI GSGKALLGRAYGPFSRVIFKDAIMG VVAPEGW AW+FK KE+NFMYVE
Sbjct: 259 SGFVFKECTIKGSGKALLGRAYGPFSRVIFKDAIMGSVVAPEGWYAWHFKGKEENFMYVE 318

Query: 330 ENCTGPGASTSMRVPWAKTLDASQLRRFSVESFINQDGWIP 349
           ENCTGPGASTSMRVPWAKTLDAS L  FSVESFINQDGWIP
Sbjct: 319 ENCTGPGASTSMRVPWAKTLDASHLTGFSVESFINQDGWIP 359

BLAST of Pay0006576 vs. TAIR 10
Match: AT2G47280.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 306.2 bits (783), Expect = 3.3e-83
Identity = 149/304 (49.01%), Postives = 199/304 (65.46%), Query Frame = 0

Query: 49  VVVDKSGRGDFQTVQAAIDSVPPNNNQWIKIQINPGVYKEKVTIPPEKPFIYLEGADRSN 108
           + VD +G G+F TVQ+AIDS+ P N+ WI++    G+Y+EKVTIP EK FIYL+G     
Sbjct: 35  ITVDLNGGGNFTTVQSAIDSISPPNHNWIRVFTQNGIYREKVTIPKEKGFIYLQGKGIEQ 94

Query: 109 TIITFDDHQQTDTSATFTSRPPNIIARGITFENSFNLEEAPELFVCDDGTYITQAIAARI 168
           T+I +DDHQ TD SATFT+   +I+  GITF+N++N+       V ++   I  A+AAR+
Sbjct: 95  TVIEYDDHQATDISATFTAFADDIVISGITFKNTYNI-------VPNNKREIVPAVAARM 154

Query: 169 YGDKSAFFNCGFKGYQDTLWDVQGRHFFQNCYIEGAIDFIFGSGQSVYKDCLINVNVA-S 228
            GD+    +  F G QDTL+D +GRH+++ C I G IDFIFG GQS++K+C +N+ +   
Sbjct: 155 LGDRYVVTDSSFVGLQDTLFDGKGRHYYKRCIISGGIDFIFGYGQSLFKECTLNMTLGIY 214

Query: 229 LPQVHQGFITAQGRRSAADPSGFVFKGCTITGSGKALLGRAYGPFSRVIFKDAIMGPVVA 288
            P    G ITA  R S +D  GFVF  CT+TG GK LLGRA+G  +RVIF  + +  VV 
Sbjct: 215 APDNPYGTITAHQRPSPSDEGGFVFSDCTVTGVGKTLLGRAWGSNARVIFDRSRLSDVVL 274

Query: 289 PEGWDAWNFKSKEKNFMYVEENCTGPGASTSMRVPWAKTLDASQLRRFSVESFINQDGWI 348
           P GWDAW  K  E++  +VE  CTG GA TS RVPW K L  S++  F+  SFI+QDGWI
Sbjct: 275 PIGWDAWRAKGNERDLTFVEAGCTGAGADTSQRVPWLKKLSLSEVDGFASVSFIDQDGWI 331

Query: 349 PRLP 352
            R P
Sbjct: 335 SRFP 331

BLAST of Pay0006576 vs. TAIR 10
Match: AT5G18990.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 298.1 bits (762), Expect = 9.1e-81
Identity = 144/303 (47.52%), Postives = 192/303 (63.37%), Query Frame = 0

Query: 49  VVVDKSGRGDFQTVQAAIDSVPPNNNQWIKIQINPGVYKEKVTIPPEKPFIYLEGADRSN 108
           V+VD+SG G+F T+Q AIDSVP NN  W  I +  G+Y+EK+TIP +KPFI + GA + +
Sbjct: 29  VIVDQSGHGNFTTIQKAIDSVPINNTHWFFINVKAGLYREKITIPQKKPFIVIVGAGKRS 88

Query: 109 TIITFDDHQQTDTSATFTSRPPNIIARGITFENSFNLEEAPELFVCDDGTYITQAIAARI 168
           T + +DDH     S TF +   N + + ITF NS+N     ++    +      A+AA I
Sbjct: 89  TRVEWDDHASLAQSPTFATLADNTVVKKITFANSYNFPSNGKI----NKNPRVPAVAAFI 148

Query: 169 YGDKSAFFNCGFKGYQDTLWDVQGRHFFQNCYIEGAIDFIFGSGQSVYKDCLINVNVASL 228
            GDKSAF++ GF G QDTLWD  GRH+F  C I+GA+DFI GSGQS+Y+ C+I V    L
Sbjct: 149 GGDKSAFYSVGFAGIQDTLWDSDGRHYFHRCTIQGAVDFILGSGQSIYQSCVIQVLGGQL 208

Query: 229 PQVHQGFITAQGRRSAADPSGFVFKGCTITGSGKALLGRAYGPFSRVIFKDAIMGPVVAP 288
                G+ITAQGR +A D +GFVF  C + G GKA LGRA+ P+SRVIF ++ +  VV P
Sbjct: 209 GPGVTGYITAQGRTNANDANGFVFINCLVHGFGKAYLGRAWRPYSRVIFYNSNLTDVVDP 268

Query: 289 EGWDAWNFKSKEKNFMYVEENCTGPGASTSMRVPWAKTLDASQLRRFSVESFINQDGWIP 348
            GW  WN++  EK   Y E  C G G++TS R  W K L AS ++  +  SFIN+ GW+ 
Sbjct: 269 LGWWEWNYQGYEKQLTYAEHGCFGSGSNTSRRAKWVKKLSASAVQHLADLSFINRGGWVE 327

Query: 349 RLP 352
            LP
Sbjct: 329 DLP 327

BLAST of Pay0006576 vs. TAIR 10
Match: AT3G24130.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 297.7 bits (761), Expect = 1.2e-80
Identity = 143/306 (46.73%), Postives = 190/306 (62.09%), Query Frame = 0

Query: 46  QSVVVVDKSGRGDFQTVQAAIDSVPPNNNQWIKIQINPGVYKEKVTIPPEKPFIYLEGAD 105
           Q  V VD+SG G+F T+Q AIDSVP NN  W  I +  G+Y+EK+ IP EKPFI L GA 
Sbjct: 31  QQQVFVDQSGHGNFTTIQKAIDSVPINNRHWFFINVKAGLYREKIKIPYEKPFIVLVGAG 90

Query: 106 RSNTIITFDDHQQTDTSATFTSRPPNIIARGITFENSFNLEEAPELFVCDDGTYITQAIA 165
           +  T + +DDH     S TF++   N + + ITF NS+N     ++    +    T A+A
Sbjct: 91  KRLTRVEWDDHYSVAQSPTFSTLADNTVVKSITFANSYNFPSKGKM----NKNPRTPAVA 150

Query: 166 ARIYGDKSAFFNCGFKGYQDTLWDVQGRHFFQNCYIEGAIDFIFGSGQSVYKDCLINVNV 225
           A I GDKSAF++ GF G QDTLWD  GRH+F  C I+GA+DFIFG+GQS+Y+ C+I V  
Sbjct: 151 ALIGGDKSAFYSVGFAGIQDTLWDFDGRHYFHRCTIQGAVDFIFGTGQSIYQSCVIQVLG 210

Query: 226 ASLPQVHQGFITAQGRRSAADPSGFVFKGCTITGSGKALLGRAYGPFSRVIFKDAIMGPV 285
             L     G+ITAQGR +  D +GF+F  C + G+G A LGR +  +SRVIF ++ +  V
Sbjct: 211 GQLEPGLAGYITAQGRTNPYDANGFIFINCLVYGTGMAFLGRPWRGYSRVIFYNSNLTDV 270

Query: 286 VAPEGWDAWNFKSKEKNFMYVEENCTGPGASTSMRVPWAKTLDASQLRRFSVESFINQDG 345
           V PEGWDAWNF   E   ++ E  C G GA+   RV W K L  S ++  +  SFIN+ G
Sbjct: 271 VVPEGWDAWNFVGHENQLVFAEHGCFGSGANIGRRVKWVKKLSESAIQNLADLSFINRGG 330

Query: 346 WIPRLP 352
           W+  LP
Sbjct: 331 WVEDLP 332

BLAST of Pay0006576 vs. TAIR 10
Match: AT2G19150.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 292.7 bits (748), Expect = 3.8e-79
Identity = 142/308 (46.10%), Postives = 192/308 (62.34%), Query Frame = 0

Query: 45  VQSVVVVDKSGRGDFQTVQAAIDSVPPNNNQWIKIQINPGVYKEKVTIPPEKPFIYLEGA 104
           +   ++V+ +    F+TVQ+AIDS+P  N  WI+I I+ G+Y EKVTIP  K +IY++G 
Sbjct: 37  IAKTIIVNPNDARYFKTVQSAIDSIPLQNQDWIRILISNGIYSEKVTIPRGKGYIYMQGG 96

Query: 105 DRSNTIITFDDHQQTDTSATFTSRPPNIIARGITFENSFNLEEAPELFVCDDGTYITQAI 164
               TII + DHQ T+TSATFTS P NII  GITF+N +N        +    +    A+
Sbjct: 97  GIEKTIIAYGDHQLTNTSATFTSYPSNIIITGITFKNKYN--------IASSSSPTKPAV 156

Query: 165 AARIYGDKSAFFNCGFKGYQDTLWDVQGRHFFQNCYIEGAIDFIFGSGQSVYKDCLINVN 224
           AA + GDK A  +  F G+QDTL+D  GRH+++ C I G IDFIFG  QS+++ C + + 
Sbjct: 157 AAMMLGDKYAIIDSSFDGFQDTLYDDYGRHYYKRCVISGGIDFIFGGAQSIFEGCTLKLR 216

Query: 225 VASLPQVH-QGFITAQGRRSAADPSGFVFKGCTITGSGKALLGRAYGPFSRVIFKDAIMG 284
           V   P     G ITAQGR S  D  GFVFK CT+ GSGKALLGRA+  +SRVIF  ++  
Sbjct: 217 VGIYPPNEVYGTITAQGRDSPTDKGGFVFKDCTVMGSGKALLGRAWKSYSRVIFYRSMFS 276

Query: 285 PVVAPEGWDAWNFKSKEKNFMYVEENCTGPGASTSMRVPWAKTLDASQLRRFSVESFINQ 344
             + P GWDAW  K +E +  +VE  CTG GA TS RVPW        + +F+  +FI++
Sbjct: 277 DNILPIGWDAWKAKGQEGHITFVEFGCTGVGADTSKRVPWLTKASEKDVLQFTNLTFIDE 336

Query: 345 DGWIPRLP 352
           +GW+ RLP
Sbjct: 337 EGWLSRLP 336

BLAST of Pay0006576 vs. TAIR 10
Match: AT5G26810.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 251.1 bits (640), Expect = 1.3e-66
Identity = 125/252 (49.60%), Postives = 164/252 (65.08%), Query Frame = 0

Query: 103 GADRSNTIITFDDHQQTDTSATFTSRPPNIIARGITFENSFNLEEAPELFVCDDGTY-IT 162
           G  +  T IT++ H  TD S+TFTS P +I+ R ++  N++N      L   +  ++ I 
Sbjct: 42  GEGQRVTTITYNGHAATDVSSTFTSYPSHIVVRNLSIMNTYN--RLTSLTKANGMSWDIK 101

Query: 163 QAIAARIYGDKSAFFNCGFKGYQDTLWDVQGRHFFQNCYIEGAIDFIFGSGQSVYKDCLI 222
            A+A  +YGDKSAF+NC F G QDT+WD  GRH F+NCYIEGAIDFIFGSGQSVY+DC I
Sbjct: 102 PAVAISVYGDKSAFYNCDFLGLQDTVWDNLGRHHFKNCYIEGAIDFIFGSGQSVYEDCHI 161

Query: 223 NVNVASL-PQVHQGFITAQGRRSAADPSGFVFKGCTITGSGKALLGRAYGPFSRVIFKDA 282
           N    +L  +V  G+ITAQGR S +DPSGFVF   +++GS    LGRAYGPFSRVIF   
Sbjct: 162 NATAGALASKVSFGYITAQGRSSDSDPSGFVFLRGSVSGSTSVYLGRAYGPFSRVIFIQT 221

Query: 283 IMGPVVAPEGWDAWNFKSKEKNFMYVEENCTGPGASTSMRVPWAKTLDASQLR-RFSVES 342
            +  VV PEGW +W++   E +F Y E  C G G+  S RVPW   L +   + +FS+ +
Sbjct: 222 DLSSVVHPEGWYSWHYGGYEMSFTYAEVECKGAGSDMSRRVPWIDKLHSFYTKQQFSISN 281

Query: 343 FINQDGWIPRLP 352
           FI+QD WI  +P
Sbjct: 282 FIDQDQWISNIP 291

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q4PSQ54.7e-8249.01Probable pectinesterase 66 OS=Arabidopsis thaliana OX=3702 GN=PME66 PE=2 SV=2[more]
Q3E9D31.3e-7947.52Probable pectinesterase 55 OS=Arabidopsis thaliana OX=3702 GN=PME55 PE=2 SV=1[more]
Q4PSN01.7e-7946.73Probable pectinesterase 29 OS=Arabidopsis thaliana OX=3702 GN=PME29 PE=2 SV=1[more]
O644795.4e-7846.10Putative pectinesterase 10 OS=Arabidopsis thaliana OX=3702 GN=PME10 PE=2 SV=1[more]
O049531.8e-6549.60Putative pectinesterase 52 OS=Arabidopsis thaliana OX=3702 GN=PME52 PE=2 SV=2[more]
Match NameE-valueIdentityDescription
A0A5A7U1G91.5e-18798.76Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold385G00148... [more]
A0A5D3BBR95.6e-18798.45Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold546G00102... [more]
A0A1S4DZS37.1e-18290.34Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103499379 PE=4 SV=1[more]
A0A0A0LH008.4e-16789.03Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_2G034610 PE=4 SV=1[more]
A0A6J1G5A01.8e-13271.43Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111450826 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
KAA0048077.13.0e-18798.76putative pectinesterase 66 [Cucumis melo var. makuwa][more]
TYJ96459.11.2e-18698.45putative pectinesterase 66 [Cucumis melo var. makuwa][more]
XP_016901459.11.5e-18190.34PREDICTED: probable pectinesterase 66 [Cucumis melo][more]
KGN61008.11.7e-16689.03hypothetical protein Csa_021278 [Cucumis sativus][more]
XP_031736683.11.2e-16283.28probable pectinesterase 66 [Cucumis sativus][more]
Match NameE-valueIdentityDescription
AT2G47280.13.3e-8349.01Pectin lyase-like superfamily protein [more]
AT5G18990.19.1e-8147.52Pectin lyase-like superfamily protein [more]
AT3G24130.11.2e-8046.73Pectin lyase-like superfamily protein [more]
AT2G19150.13.8e-7946.10Pectin lyase-like superfamily protein [more]
AT5G26810.11.3e-6649.60Pectin lyase-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (Payzawat) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 42..350
e-value: 4.6E-105
score: 353.5
IPR000070Pectinesterase, catalyticPFAMPF01095Pectinesterasecoord: 49..343
e-value: 3.1E-63
score: 213.3
NoneNo IPR availablePANTHERPTHR31321ACYL-COA THIOESTER HYDROLASE YBHC-RELATEDcoord: 43..351
NoneNo IPR availablePANTHERPTHR31321:SF82PECTINESTERASEcoord: 43..351
IPR033131Pectinesterase, Asp active sitePROSITEPS00503PECTINESTERASE_2coord: 201..210
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 45..349

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Pay0006576.1Pay0006576.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042545 cell wall modification
biological_process GO:0045490 pectin catabolic process
molecular_function GO:0045330 aspartyl esterase activity
molecular_function GO:0030599 pectinesterase activity