Pay0003457 (gene) Melon (Payzawat) v1

Overview


Name	Pay0003457
Type	gene
Organism	Cucumis melo L. var. inodorus cv. Payzawat (Melon (Payzawat) v1)
Description	protein MIS12 homolog
Location	chr02: 22401985 .. 22405177 (-)
RNA-Seq Expression	Pay0003457
Synteny	Pay0003457

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TTACCATTTTGGCGGCAAAATTCCTCCATTTTTGCCAAAGCTTTGGAGCTTCCCCTTCCCATTTGAATTTCTCCTCCTCCTCTTCTTCTTCTTCTTTGGGTAAGAAGGCTTTTCAGGAAGCAAAAGGGTAATTTTGATGGAAGGAAGTAAAGGCGAATCGGTATTCAATTCCCTCAATCTCAATCCACAGCTCTTCATTAATGAAGCTCTCAATACTGTCGACGATTTGGTTGATGATGCTTTCGATTTCTATCAATCGTATTGCTCTCTCTGTTTTCCATTTCTTTAACTTAGGTTTTTCAATTTTGTTCCGTTAATTTTGTTAATTCTTTTTCCCTTCAGACAAGCATCTGCTGCCTTGAAGACCGAGGGCTCCGATAGATCTCAGGATCTTACCCTGGTAAGATTTAATTTGAGTTATGTTATTTGGCATTTTCTTGTTAATTTCTGAATTCTTAAGTTTGTACTCTTAAGCCCTTCTTTGAATTGTTCCCATTTTCTTTGAACAGAAATTGTTTATTTGCCTCGTTTTATGTAGGGAATATCGCAAGTCCGAGCGTTGGTCCAATTGGGTCTGGATAAGCGATTGGCCATGTGGGAAAAGTACTGTCTTAATCACTGTTTTTCAGTGCCTGAAGGTTTTTCTTTACCCTCAAATGTAGGTTCTTCTTCTACTCCGTCCGTAATGAGGCTCCTTTGCCAAAGAATAAAATATCCACGTCGGTATCAATATCTGAATTTTAGTTTGTAGTTTATCGATTATATATCAACATTGACAGATATCATTGCAAAACAATAAATTAACAGGAATTGTTTAAATTAGCTATTGAATTGCTTTTGCTTTCAAACTAAGTTGTAGATCTTGCTTTTAAGTAATATTCATATTGAGATAAATAGATGATATCTTTTCATGTTTTTATGTGTTTACAAAAGATAGTTGACATGTTTATAAACCCTTAATATCGATGTCATACCTTCACATATATATATATATATATATATATATATATATATATATAACTTTGCTTGTTCAAAAGTTCTCTCTATATATAACTTAGCATGTAAATTGGCTGGTTGCTTATGCAGCGAGTTTCCCATTTATTTTGTACTGACTTGATAGCTTCCTCAATTTTTTTGTCTGGGACGAACAAATTTTGAGGAGAAAGCCCAGTTACTGTGCATCATTGAGTTTCAGATGTAATCACGAGTTTCCATATTTTCAGACCTCAGAGCTTCCTCATTTTGTTAGCAATAACTTAACGTGGTTCGATTGTAACTAAAATGTGTTCGTGACAGTAAAAGATTCTTTTTAGCTCTAAAAGGAATGCGACCAAATGCCTGCAGCACTAGGAATGCTAAATTAGTGGCTGATTAGTTGTTCCCGAAGAGGCAGTTGCACTTGTTTATGCTTTTCCCTTCTTTCGTCAAGTAGTTCAACAAAGAGTCTCCCTTTCGCAGGCCATAAGTGTCTCGCTTGCTTATTTCTGTCTGAATTCCCTTCTTTTTCGGTCTAAAAATCTGTCACTGGGGCATCATGCCTTTTCTTTTTCAATTATATTTGAAGTCTCCTATGTAAGGTCCAGTTCGATTCCTTTCATTTTGCACCAATATAGTTTACTTGGGGTTGGGCAACTTCTGTTTGTTTCACTGGCATCTCATTGAAATCCACAATATCTTATTGTCAGGATGCTAAATTATCAATGTTTCCCCCCTTGTTTGAGAACTTAGAGTTATGGCTAATTGAGTCTGTGTTCTTTTACTACTTTTTCTTCTTTCTAATGTAATAGCCGATAAACATATTTCTGATTCAGATCTAAATGTTTTCTTTAAGTTGTCTTAGGATGAATCACCTGGTGTCACTTCAATAAATCATGACCAAGACGTGGATCTGGATACAGAGCTAGATCTTTTGAGAAATAAGCTCTCCGAGGTATTGAATATGTCGTATGTTTAACATGTGCGTTATGTAAATCATATTTCATGAGGAAATATATTCTTCAGGTCAGAAAAGAGAATATTGTATTAAACCAAGAGCTACAAGCTTTGGAAAGGCAAACTGCTTCCAGTAACTCCCAAATCAGTCATTTCAACGAAGCATTACAACTATATGAGCAAAGTTCTGTGAATGACATGTTCCAGGGTAATTAAAGATGTGCTTAAAACCAATTTTTTCTTCTTGCACTCCTGGGTTTACATGCACACTACTCTACTAGTACACTCATGAAGGCATCCTCGAAATGAAGTATACAAATGTGATAGTTCCAAATGACACTTAAGTTTGACACCTATAAAATCATTTCATTTATGCAATTTTTATGTTACCCTAAACTCTAATTGTCTCATTTTCTGTTCATTCCATAAAAGAGGAGCTTCTTGCAGTATGTGAATAAGGCAACATCTCTTGCTATTTTCATTTTGATTGCCACTTGGATAAAATTTATTCATTAGTTATTAAATTTCGTTGTACTAAAACTTCAAAAGTCGGTAGGTAAAAGTTGTTCATTGTTGTAGGAAGCAGAATTCTAACATGTATCAATTTCTTTCAGAAATGATCAAAACTGCATCAGAGCTCCGTGCAAAAATAGGAAAGATGAAGAAAAGGAGGACGGAGGAAGCCAAGCTCGCTAAAGTAGAGAAGGTTCATACAAATGGAGACATCTCTCACCATCACAATGGTAGGATTTTCAAGCTACTTTATTATCAATGAAGCTGGTTTTAGCTTTTTTGAAGCTGAATATCTTCTCACATGCTCGTTGATGCTTGTTTCTGTCGTGCAGGTTTCTCTAATGCTAAGCTGGATGATATTCAAGAATTTTTATCTGATTTGAAGAAGATTTGAAGTTATTTCACCATGACTTCAATCTTACTGTATATCATGCTTTTCGAAACTTGTCACTATGAGTTCTTCAATGTTTAAACTTTATTTAGCAGTTGTGAGATATTCCAAACCAAATTTGTATGATATATCATTTGGATTGATGACTATCCTTAGCAATCGCCTTAGAGAGTTTGCAGATGAGCCATGACTAGTTGGCTTAATCCTTCGTAAATCAATTGTTATGGTTTGTATTACACTTTTGAAGCTTATACTTTTGTTTAAAAAATATTATCAAACTTTCAAAAAGTTGTAAATGGAATTACTGGTAATTGGAAGGTGTTGGAATGAATGGAGAATCAACTGGGTAGATGGGAA

mRNA sequence

TTACCATTTTGGCGGCAAAATTCCTCCATTTTTGCCAAAGCTTTGGAGCTTCCCCTTCCCATTTGAATTTCTCCTCCTCCTCTTCTTCTTCTTCTTTGGGTAAGAAGGCTTTTCAGGAAGCAAAAGGGTAATTTTGATGGAAGGAAGTAAAGGCGAATCGGTATTCAATTCCCTCAATCTCAATCCACAGCTCTTCATTAATGAAGCTCTCAATACTGTCGACGATTTGGTTGATGATGCTTTCGATTTCTATCAATCACAAGCATCTGCTGCCTTGAAGACCGAGGGCTCCGATAGATCTCAGGATCTTACCCTGGGAATATCGCAAGTCCGAGCGTTGGTCCAATTGGGTCTGGATAAGCGATTGGCCATGTGGGAAAAGTACTGTCTTAATCACTGTTTTTCAGTGCCTGAAGGTTTTTCTTTACCCTCAAATGATGAATCACCTGGTGTCACTTCAATAAATCATGACCAAGACGTGGATCTGGATACAGAGCTAGATCTTTTGAGAAATAAGCTCTCCGAGGTCAGAAAAGAGAATATTGTATTAAACCAAGAGCTACAAGCTTTGGAAAGGCAAACTGCTTCCAGTAACTCCCAAATCAGTCATTTCAACGAAGCATTACAACTATATGAGCAAAGTTCTGTGAATGACATGTTCCAGGAAATGATCAAAACTGCATCAGAGCTCCGTGCAAAAATAGGAAAGATGAAGAAAAGGAGGACGGAGGAAGCCAAGCTCGCTAAAGTAGAGAAGGTTCATACAAATGGAGACATCTCTCACCATCACAATGGTTTCTCTAATGCTAAGCTGGATGATATTCAAGAATTTTTATCTGATTTGAAGAAGATTTGAAGTTATTTCACCATGACTTCAATCTTACTGTATATCATGCTTTTCGAAACTTGTCACTATGAGTTCTTCAATGTTTAAACTTTATTTAGCAGTTGTGAGATATTCCAAACCAAATTTGTATGATATATCATTTGGATTGATGACTATCCTTAGCAATCGCCTTAGAGAGTTTGCAGATGAGCCATGACTAGTTGGCTTAATCCTTCGTAAATCAATTGTTATGGTTTGTATTACACTTTTGAAGCTTATACTTTTGTTTAAAAAATATTATCAAACTTTCAAAAAGTTGTAAATGGAATTACTGGTAATTGGAAGGTGTTGGAATGAATGGAGAATCAACTGGGTAGATGGGAA

Coding sequence (CDS)

ATGGAAGGAAGTAAAGGCGAATCGGTATTCAATTCCCTCAATCTCAATCCACAGCTCTTCATTAATGAAGCTCTCAATACTGTCGACGATTTGGTTGATGATGCTTTCGATTTCTATCAATCACAAGCATCTGCTGCCTTGAAGACCGAGGGCTCCGATAGATCTCAGGATCTTACCCTGGGAATATCGCAAGTCCGAGCGTTGGTCCAATTGGGTCTGGATAAGCGATTGGCCATGTGGGAAAAGTACTGTCTTAATCACTGTTTTTCAGTGCCTGAAGGTTTTTCTTTACCCTCAAATGATGAATCACCTGGTGTCACTTCAATAAATCATGACCAAGACGTGGATCTGGATACAGAGCTAGATCTTTTGAGAAATAAGCTCTCCGAGGTCAGAAAAGAGAATATTGTATTAAACCAAGAGCTACAAGCTTTGGAAAGGCAAACTGCTTCCAGTAACTCCCAAATCAGTCATTTCAACGAAGCATTACAACTATATGAGCAAAGTTCTGTGAATGACATGTTCCAGGAAATGATCAAAACTGCATCAGAGCTCCGTGCAAAAATAGGAAAGATGAAGAAAAGGAGGACGGAGGAAGCCAAGCTCGCTAAAGTAGAGAAGGTTCATACAAATGGAGACATCTCTCACCATCACAATGGTTTCTCTAATGCTAAGCTGGATGATATTCAAGAATTTTTATCTGATTTGAAGAAGATTTGA

Protein sequence

MEGSKGESVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTEGSDRSQDLTLGISQVRALVQLGLDKRLAMWEKYCLNHCFSVPEGFSLPSNDESPGVTSINHDQDVDLDTELDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQEMIKTASELRAKIGKMKKRRTEEAKLAKVEKVHTNGDISHHHNGFSNAKLDDIQEFLSDLKKI

Homology

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BLAST of Pay0003457 vs. ExPASy Swiss-Prot
Match: Q2V0Z5 (Protein MIS12 homolog OS=Arabidopsis thaliana OX=3702 GN=MIS12 PE=3 SV=1)

HSP 1 Score: 207.6 bits (527), Expect = 1.6e-52
Identity = 117/247 (47.37%), Postives = 176/247 (71.26%), Query Frame = 0


Query: 1   MEGSKGESVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTEGSD--RSQDL 60
           MEGSK E+VF+S+NLNPQ+FINEA+N+V+D VD AFDFY   AS +LK +GSD  +SQ L
Sbjct: 1   MEGSKSEAVFDSMNLNPQIFINEAINSVEDYVDQAFDFYARDASKSLKIKGSDKQKSQAL 60

Query: 61  TLGISQVRALVQLGLDKRLAMWEKYCLNHCFSVPEGFSLPSNDESPGVTSINHD--QDVD 120
           + GI++VR L+   +D RL +WE Y L  CF+VP+GF LP ++ES   +S++ D   D++
Sbjct: 61  SNGIARVRGLLLSVIDNRLKLWESYSLRFCFAVPDGFVLPKSEES---SSVHQDGLYDLE 120

Query: 121 LDTELDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQ 180
           LD ELD LR+KL+ V K ++ L+ ELQALER + S    +   NEAL+LY++SS++++F+
Sbjct: 121 LDAELDSLRDKLNVVGKRSVELDSELQALERSSVSRERSLRLVNEALELYDESSMDEIFK 180

Query: 181 EMIKTASELRAKIGKMKKRRTEEAKLAKVEKVHTNGDISHHHNGFS----NAKLDDIQEF 240
           EM K ASELRA + ++K RR + ++ AKV++      + +H   FS    + KL+D+++F
Sbjct: 181 EMTKMASELRASVERLKTRRMKASESAKVKR------LKNHGKEFSAMTFDGKLEDLEKF 238

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BLAST of Pay0003457 vs. ExPASy TrEMBL
Match: A0A5A7US57 (Centromere protein Mis12 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold629G00040 PE=3 SV=1)

HSP 1 Score: 459.9 bits (1182), Expect = 6.4e-126
Identity = 239/239 (100.00%), Postives = 239/239 (100.00%), Query Frame = 0


Query: 1   MEGSKGESVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTEGSDRSQDLTL 60
           MEGSKGESVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTEGSDRSQDLTL
Sbjct: 1   MEGSKGESVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTEGSDRSQDLTL 60

Query: 61  GISQVRALVQLGLDKRLAMWEKYCLNHCFSVPEGFSLPSNDESPGVTSINHDQDVDLDTE 120
           GISQVRALVQLGLDKRLAMWEKYCLNHCFSVPEGFSLPSNDESPGVTSINHDQDVDLDTE
Sbjct: 61  GISQVRALVQLGLDKRLAMWEKYCLNHCFSVPEGFSLPSNDESPGVTSINHDQDVDLDTE 120

Query: 121 LDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQEMIK 180
           LDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQEMIK
Sbjct: 121 LDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQEMIK 180

Query: 181 TASELRAKIGKMKKRRTEEAKLAKVEKVHTNGDISHHHNGFSNAKLDDIQEFLSDLKKI 240
           TASELRAKIGKMKKRRTEEAKLAKVEKVHTNGDISHHHNGFSNAKLDDIQEFLSDLKKI
Sbjct: 181 TASELRAKIGKMKKRRTEEAKLAKVEKVHTNGDISHHHNGFSNAKLDDIQEFLSDLKKI 239

[Close]

BLAST of Pay0003457 vs. ExPASy TrEMBL
Match: A0A5A7UQ95 (Centromere protein Mis12 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold274G003370 PE=3 SV=1)

HSP 1 Score: 457.2 bits (1175), Expect = 4.2e-125
Identity = 237/239 (99.16%), Postives = 238/239 (99.58%), Query Frame = 0


Query: 1   MEGSKGESVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTEGSDRSQDLTL 60
           MEGSKGESVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASA LKTEGSDRSQDLTL
Sbjct: 1   MEGSKGESVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASATLKTEGSDRSQDLTL 60

Query: 61  GISQVRALVQLGLDKRLAMWEKYCLNHCFSVPEGFSLPSNDESPGVTSINHDQDVDLDTE 120
           GISQVRALVQLGLDKRLAMWEKYCLNHCFSVPEGFSLPSNDESPGVTSINHDQDVDLDTE
Sbjct: 61  GISQVRALVQLGLDKRLAMWEKYCLNHCFSVPEGFSLPSNDESPGVTSINHDQDVDLDTE 120

Query: 121 LDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQEMIK 180
           LDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQEMIK
Sbjct: 121 LDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQEMIK 180

Query: 181 TASELRAKIGKMKKRRTEEAKLAKVEKVHTNGDISHHHNGFSNAKLDDIQEFLSDLKKI 240
           TASELR+KIGKMKKRRTEEAKLAKVEKVHTNGDISHHHNGFSNAKLDDIQEFLSDLKKI
Sbjct: 181 TASELRSKIGKMKKRRTEEAKLAKVEKVHTNGDISHHHNGFSNAKLDDIQEFLSDLKKI 239

[Close]

BLAST of Pay0003457 vs. ExPASy TrEMBL
Match: A0A0A0LPL2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G014450 PE=3 SV=1)

HSP 1 Score: 427.2 bits (1097), Expect = 4.6e-116
Identity = 225/239 (94.14%), Postives = 229/239 (95.82%), Query Frame = 0


Query: 1   MEGSKGESVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTEGSDRSQDLTL 60
           MEGSKGE VFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTE SDRSQDLTL
Sbjct: 1   MEGSKGEVVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTESSDRSQDLTL 60

Query: 61  GISQVRALVQLGLDKRLAMWEKYCLNHCFSVPEGFSLPSNDESPGVTSINHDQDVDLDTE 120
           GISQVRALVQLGL KRLAMWEKYCLNHCFSVPEGFSLPS+DESPGVTSI+HD DVDLDTE
Sbjct: 61  GISQVRALVQLGLHKRLAMWEKYCLNHCFSVPEGFSLPSDDESPGVTSISHDHDVDLDTE 120

Query: 121 LDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQEMIK 180
           LDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQEMI+
Sbjct: 121 LDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQEMIR 180

Query: 181 TASELRAKIGKMKKRRTEEAKLAKVEKVHTNGDISHHHNGFSNAKLDDIQEFLSDLKKI 240
           TASELR KIGK+KK RTEE KLA VEKVHTNGDISHHH GFSNAKLDDIQEFLS LKKI
Sbjct: 181 TASELRVKIGKLKK-RTEETKLAIVEKVHTNGDISHHHKGFSNAKLDDIQEFLSGLKKI 238

[Close]

BLAST of Pay0003457 vs. ExPASy TrEMBL
Match: A0A6J1E0A1 (protein MIS12 homolog OS=Cucurbita moschata OX=3662 GN=LOC111429660 PE=3 SV=1)

HSP 1 Score: 395.2 bits (1014), Expect = 1.9e-106
Identity = 204/241 (84.65%), Postives = 221/241 (91.70%), Query Frame = 0


Query: 1   MEGSKGESVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTEGSDRSQDLTL 60
           MEGSKGE VF+SLNLNPQLFINEALN VDDLVDDAFDFYQ+QASA+LKTEGSDRSQDL L
Sbjct: 1   MEGSKGEEVFDSLNLNPQLFINEALNIVDDLVDDAFDFYQTQASASLKTEGSDRSQDLIL 60

Query: 61  GISQVRALVQLGLDKRLAMWEKYCLNHCFSVPEGFSLPSNDESPGVTSINHD--QDVDLD 120
           GIS+VR  VQ GLDKRLAMWEKYCLNHCFSVPEGFSLPSNDESP VTS++ D   DVDLD
Sbjct: 61  GISRVRTFVQSGLDKRLAMWEKYCLNHCFSVPEGFSLPSNDESPVVTSVSRDCLYDVDLD 120

Query: 121 TELDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQEM 180
           TELD LRNKLSEVRKENIVLNQELQALERQ ASSNSQ++ FNEALQLYE++SVN+MFQEM
Sbjct: 121 TELDFLRNKLSEVRKENIVLNQELQALERQAASSNSQVNRFNEALQLYERNSVNEMFQEM 180

Query: 181 IKTASELRAKIGKMKKRRTEEAKLAKVEKVHTNGDISHHHNGFSNAKLDDIQEFLSDLKK 240
           + TASELRAKIGK+KKRR EE+KL KVEK+HTNGD SHHH GFSNAKLDDIQEFL+DLK 
Sbjct: 181 MGTASELRAKIGKLKKRRMEESKLTKVEKIHTNGDASHHHKGFSNAKLDDIQEFLADLKA 240

[Close]

BLAST of Pay0003457 vs. ExPASy TrEMBL
Match: A0A6J1JME3 (protein MIS12 homolog isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111485708 PE=3 SV=1)

HSP 1 Score: 393.3 bits (1009), Expect = 7.4e-106
Identity = 204/241 (84.65%), Postives = 220/241 (91.29%), Query Frame = 0


Query: 1   MEGSKGESVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTEGSDRSQDLTL 60
           MEGSKGE VF+SLNLNPQLFINEALN VDDLVDDAFDFYQ+QASA+LKTEGSDRSQDL L
Sbjct: 1   MEGSKGEEVFDSLNLNPQLFINEALNIVDDLVDDAFDFYQTQASASLKTEGSDRSQDLIL 60

Query: 61  GISQVRALVQLGLDKRLAMWEKYCLNHCFSVPEGFSLPSNDESPGVTSINHD--QDVDLD 120
           GIS+VR  VQ GLDKRLAMWEKYCLNHCFSVPEGFSLPSNDESP VTS++ D   DVDLD
Sbjct: 61  GISRVRTFVQSGLDKRLAMWEKYCLNHCFSVPEGFSLPSNDESPVVTSVSRDCLYDVDLD 120

Query: 121 TELDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQEM 180
           TELD LR  LSEVRKENIVLNQELQALERQTASSNSQ++ FNEALQLYEQ+SVN+MFQEM
Sbjct: 121 TELDFLRKNLSEVRKENIVLNQELQALERQTASSNSQVNRFNEALQLYEQNSVNEMFQEM 180

Query: 181 IKTASELRAKIGKMKKRRTEEAKLAKVEKVHTNGDISHHHNGFSNAKLDDIQEFLSDLKK 240
           + TASELRAKIGK+KKRR EE+KL KVEK+HTN DISHHH GFSNAKLDDIQEFL+DLK 
Sbjct: 181 MGTASELRAKIGKLKKRRMEESKLTKVEKIHTNRDISHHHKGFSNAKLDDIQEFLADLKT 240

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BLAST of Pay0003457 vs. NCBI nr
Match: KAA0058004.1 (Centromere protein Mis12 [Cucumis melo var. makuwa] >TYK28378.1 Centromere protein Mis12 [Cucumis melo var. makuwa])

HSP 1 Score: 459.9 bits (1182), Expect = 1.3e-125
Identity = 239/239 (100.00%), Postives = 239/239 (100.00%), Query Frame = 0


Query: 1   MEGSKGESVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTEGSDRSQDLTL 60
           MEGSKGESVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTEGSDRSQDLTL
Sbjct: 1   MEGSKGESVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTEGSDRSQDLTL 60

Query: 61  GISQVRALVQLGLDKRLAMWEKYCLNHCFSVPEGFSLPSNDESPGVTSINHDQDVDLDTE 120
           GISQVRALVQLGLDKRLAMWEKYCLNHCFSVPEGFSLPSNDESPGVTSINHDQDVDLDTE
Sbjct: 61  GISQVRALVQLGLDKRLAMWEKYCLNHCFSVPEGFSLPSNDESPGVTSINHDQDVDLDTE 120

Query: 121 LDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQEMIK 180
           LDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQEMIK
Sbjct: 121 LDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQEMIK 180

Query: 181 TASELRAKIGKMKKRRTEEAKLAKVEKVHTNGDISHHHNGFSNAKLDDIQEFLSDLKKI 240
           TASELRAKIGKMKKRRTEEAKLAKVEKVHTNGDISHHHNGFSNAKLDDIQEFLSDLKKI
Sbjct: 181 TASELRAKIGKMKKRRTEEAKLAKVEKVHTNGDISHHHNGFSNAKLDDIQEFLSDLKKI 239

[Close]

BLAST of Pay0003457 vs. NCBI nr
Match: KAA0058032.1 (Centromere protein Mis12 [Cucumis melo var. makuwa])

HSP 1 Score: 457.2 bits (1175), Expect = 8.6e-125
Identity = 237/239 (99.16%), Postives = 238/239 (99.58%), Query Frame = 0


Query: 1   MEGSKGESVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTEGSDRSQDLTL 60
           MEGSKGESVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASA LKTEGSDRSQDLTL
Sbjct: 1   MEGSKGESVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASATLKTEGSDRSQDLTL 60

Query: 61  GISQVRALVQLGLDKRLAMWEKYCLNHCFSVPEGFSLPSNDESPGVTSINHDQDVDLDTE 120
           GISQVRALVQLGLDKRLAMWEKYCLNHCFSVPEGFSLPSNDESPGVTSINHDQDVDLDTE
Sbjct: 61  GISQVRALVQLGLDKRLAMWEKYCLNHCFSVPEGFSLPSNDESPGVTSINHDQDVDLDTE 120

Query: 121 LDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQEMIK 180
           LDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQEMIK
Sbjct: 121 LDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQEMIK 180

Query: 181 TASELRAKIGKMKKRRTEEAKLAKVEKVHTNGDISHHHNGFSNAKLDDIQEFLSDLKKI 240
           TASELR+KIGKMKKRRTEEAKLAKVEKVHTNGDISHHHNGFSNAKLDDIQEFLSDLKKI
Sbjct: 181 TASELRSKIGKMKKRRTEEAKLAKVEKVHTNGDISHHHNGFSNAKLDDIQEFLSDLKKI 239

[Close]

BLAST of Pay0003457 vs. NCBI nr
Match: XP_004138204.3 (protein MIS12 homolog isoform X1 [Cucumis sativus])

HSP 1 Score: 429.9 bits (1104), Expect = 1.5e-116
Identity = 226/239 (94.56%), Postives = 230/239 (96.23%), Query Frame = 0


Query: 1   MEGSKGESVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTEGSDRSQDLTL 60
           MEGSKGE VFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTE SDRSQDLTL
Sbjct: 1   MEGSKGEVVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTESSDRSQDLTL 60

Query: 61  GISQVRALVQLGLDKRLAMWEKYCLNHCFSVPEGFSLPSNDESPGVTSINHDQDVDLDTE 120
           GISQVRALVQLGL KRLAMWEKYCLNHCFSVPEGFSLPS+DESPGVTSI+HD DVDLDTE
Sbjct: 61  GISQVRALVQLGLHKRLAMWEKYCLNHCFSVPEGFSLPSDDESPGVTSISHDHDVDLDTE 120

Query: 121 LDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQEMIK 180
           LDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQEMI+
Sbjct: 121 LDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQEMIR 180

Query: 181 TASELRAKIGKMKKRRTEEAKLAKVEKVHTNGDISHHHNGFSNAKLDDIQEFLSDLKKI 240
           TASELR KIGK+KK RTEE KLA VEKVHTNGDISHHH GFSNAKLDDIQEFLSDLKKI
Sbjct: 181 TASELRVKIGKLKK-RTEETKLAIVEKVHTNGDISHHHKGFSNAKLDDIQEFLSDLKKI 238

[Close]

BLAST of Pay0003457 vs. NCBI nr
Match: XP_038879945.1 (protein MIS12 homolog [Benincasa hispida])

HSP 1 Score: 410.2 bits (1053), Expect = 1.2e-110
Identity = 211/239 (88.28%), Postives = 226/239 (94.56%), Query Frame = 0


Query: 1   MEGSKGESVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTEGSDRSQDLTL 60
           MEGSKGE+VF+SLNLNPQLFINEALN VDDLVDDAF FYQSQASA LKTEGSDRSQDLTL
Sbjct: 31  MEGSKGEAVFDSLNLNPQLFINEALNIVDDLVDDAFGFYQSQASATLKTEGSDRSQDLTL 90

Query: 61  GISQVRALVQLGLDKRLAMWEKYCLNHCFSVPEGFSLPSNDESPGVTSINHDQDVDLDTE 120
           GIS+VRALVQLGLDKRLAMWEKYCLNHCFSVPEGFSLPSND+SPGVTSI+HD DV LDTE
Sbjct: 91  GISRVRALVQLGLDKRLAMWEKYCLNHCFSVPEGFSLPSNDDSPGVTSISHDYDVGLDTE 150

Query: 121 LDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQEMIK 180
           LD LRNKLSEVRKENIVLNQELQALERQTA+SNSQI+HFNEALQLYEQ+SVN+MFQEM+ 
Sbjct: 151 LDFLRNKLSEVRKENIVLNQELQALERQTATSNSQINHFNEALQLYEQNSVNEMFQEMMG 210

Query: 181 TASELRAKIGKMKKRRTEEAKLAKVEKVHTNGDISHHHNGFSNAKLDDIQEFLSDLKKI 240
           TASELRAKIGK+KKRR EE+KL KVEKVHTNGDISH+HNG SNAKLDDI+ FL DLK +
Sbjct: 211 TASELRAKIGKLKKRRMEESKLTKVEKVHTNGDISHYHNGLSNAKLDDIKIFLDDLKTL 269

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BLAST of Pay0003457 vs. NCBI nr
Match: XP_023515499.1 (protein MIS12 homolog isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 401.0 bits (1029), Expect = 7.3e-108
Identity = 206/241 (85.48%), Postives = 224/241 (92.95%), Query Frame = 0


Query: 1   MEGSKGESVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTEGSDRSQDLTL 60
           MEGSKGE VF+SLNLNPQLFINEALN VDDLVDDAFDFYQ+QASA+LKTEGSDRSQDL+L
Sbjct: 1   MEGSKGEEVFDSLNLNPQLFINEALNIVDDLVDDAFDFYQTQASASLKTEGSDRSQDLSL 60

Query: 61  GISQVRALVQLGLDKRLAMWEKYCLNHCFSVPEGFSLPSNDESPGVTSINHD--QDVDLD 120
           GIS+VR  VQ GLDKRLAMWEKYCLNHCFSVPEGFSLPSNDESP VTS++ D   DVDLD
Sbjct: 61  GISRVRTFVQSGLDKRLAMWEKYCLNHCFSVPEGFSLPSNDESPVVTSVSRDCLYDVDLD 120

Query: 121 TELDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQEM 180
           TELD LRNKLSEVRKEN+VLNQELQALERQTASSNSQ++ FNEALQLYEQ+SVN+MFQEM
Sbjct: 121 TELDFLRNKLSEVRKENVVLNQELQALERQTASSNSQVNRFNEALQLYEQNSVNEMFQEM 180

Query: 181 IKTASELRAKIGKMKKRRTEEAKLAKVEKVHTNGDISHHHNGFSNAKLDDIQEFLSDLKK 240
           + TASELRAKIGK+KKRR EE+KL KVEK+HTNGDISHHH GFSNAKLDDIQEFL+DLK 
Sbjct: 181 MGTASELRAKIGKLKKRRMEESKLTKVEKIHTNGDISHHHKGFSNAKLDDIQEFLADLKT 240

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BLAST of Pay0003457 vs. TAIR 10
Match: AT5G35520.1 (minichromosome instability 12 (mis12)-like )

HSP 1 Score: 207.6 bits (527), Expect = 1.1e-53
Identity = 117/247 (47.37%), Postives = 176/247 (71.26%), Query Frame = 0


Query: 1   MEGSKGESVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTEGSD--RSQDL 60
           MEGSK E+VF+S+NLNPQ+FINEA+N+V+D VD AFDFY   AS +LK +GSD  +SQ L
Sbjct: 1   MEGSKSEAVFDSMNLNPQIFINEAINSVEDYVDQAFDFYARDASKSLKIKGSDKQKSQAL 60

Query: 61  TLGISQVRALVQLGLDKRLAMWEKYCLNHCFSVPEGFSLPSNDESPGVTSINHD--QDVD 120
           + GI++VR L+   +D RL +WE Y L  CF+VP+GF LP ++ES   +S++ D   D++
Sbjct: 61  SNGIARVRGLLLSVIDNRLKLWESYSLRFCFAVPDGFVLPKSEES---SSVHQDGLYDLE 120

Query: 121 LDTELDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQ 180
           LD ELD LR+KL+ V K ++ L+ ELQALER + S    +   NEAL+LY++SS++++F+
Sbjct: 121 LDAELDSLRDKLNVVGKRSVELDSELQALERSSVSRERSLRLVNEALELYDESSMDEIFK 180

Query: 181 EMIKTASELRAKIGKMKKRRTEEAKLAKVEKVHTNGDISHHHNGFS----NAKLDDIQEF 240
           EM K ASELRA + ++K RR + ++ AKV++      + +H   FS    + KL+D+++F
Sbjct: 181 EMTKMASELRASVERLKTRRMKASESAKVKR------LKNHGKEFSAMTFDGKLEDLEKF 238

The following BLAST results are available for this feature:

BLAST of Pay0003457 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q2V0Z5	1.6e-52	47.37	Protein MIS12 homolog OS=Arabidopsis thaliana OX=3702 GN=MIS12 PE=3 SV=1	[more]

BLAST of Pay0003457 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7US57	6.4e-126	100.00	Centromere protein Mis12 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffol...	[more]
A0A5A7UQ95	4.2e-125	99.16	Centromere protein Mis12 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffol...	[more]
A0A0A0LPL2	4.6e-116	94.14	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G014450 PE=3 SV=1	[more]
A0A6J1E0A1	1.9e-106	84.65	protein MIS12 homolog OS=Cucurbita moschata OX=3662 GN=LOC111429660 PE=3 SV=1	[more]
A0A6J1JME3	7.4e-106	84.65	protein MIS12 homolog isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111485708 PE=...	[more]

BLAST of Pay0003457 vs. NCBI nr


Match Name	E-value	Identity	Description
KAA0058004.1	1.3e-125	100.00	Centromere protein Mis12 [Cucumis melo var. makuwa] >TYK28378.1 Centromere prote...	[more]
KAA0058032.1	8.6e-125	99.16	Centromere protein Mis12 [Cucumis melo var. makuwa]	[more]
XP_004138204.3	1.5e-116	94.56	protein MIS12 homolog isoform X1 [Cucumis sativus]	[more]
XP_038879945.1	1.2e-110	88.28	protein MIS12 homolog [Benincasa hispida]	[more]
XP_023515499.1	7.3e-108	85.48	protein MIS12 homolog isoform X2 [Cucurbita pepo subsp. pepo]	[more]

BLAST of Pay0003457 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G35520.1	1.1e-53	47.37	minichromosome instability 12 (mis12)-like	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (Payzawat) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 121..148
IPR008685	Centromere protein Mis12	PFAM	PF05859	Mis12	coord: 11..139 e-value: 2.4E-20 score: 73.1
IPR008685	Centromere protein Mis12	PANTHER	PTHR14527	PROTEIN MIS12 HOMOLOG	coord: 1..238

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Pay0003457.1	Pay0003457.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0051301	cell division
biological_process	GO:0051382	kinetochore assembly
biological_process	GO:0000070	mitotic sister chromatid segregation
biological_process	GO:0034501	protein localization to kinetochore
biological_process	GO:0000278	mitotic cell cycle
cellular_component	GO:0000818	nuclear MIS12/MIND complex
cellular_component	GO:0000775	chromosome, centromeric region
cellular_component	GO:0005634	nucleus