Pay0001623 (gene) Melon (Payzawat) v1

Overview
NamePay0001623
Typegene
OrganismCucumis melo L. var. inodorus cv. Payzawat (Melon (Payzawat) v1)
DescriptionRegulator of Vps4 activity in the MVB pathway protein, putative
Locationchr01: 34782034 .. 34785102 (-)
RNA-Seq ExpressionPay0001623
SyntenyPay0001623
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCCTCACAGCTGGGATCATCCATTACCTTTTTATCGGACTTCTTCTTCTTCTTCTTCTTCATTCACATTTCTTCTTTTCTTTTACTGTTTTCTCATTTGGGGGTTTCTTTTTTGTCCCTTTTTTTTGGGGATACTTTTTGTTTTTGTCGTTAAGCTTCTCTGTTTTCTCTTGCTCTGTCGTTTCTGTGTTTCGTTTATGTTATTCCATTTGGTTGATTATTCATCGTCCTTTTTTATTATTTCTTGAAGATCCTTCTATGGTGGATGTGGGACTGTTCTATTTTCATTCCTTCTTATAACTGTCCTTCATTCGGGGTTGTTCTTTCTTGGCGTCTGGTTTGTTTTTTTGATCTTTAGTTTCTTGTGCTTATGGGTTTCTTGGTTTTAGATTCTTAGTACATTTTTACCGATTTCAATTTGGGAAATTCTTGTTTGCCTTTGTTGTTCGGGATTTTGGATTCCCCATTTCCCTGATTCTTCGGTTTTCTTCCTTATTTTAACCCCCTAAAATTCCGTTTCCTGTCAATTTTGCTTTGCGATCACAATGTTCGACATGTTCTTGAAGCCTAAGTTCTATACAAAATGGTATTTTTGCAGCCTTTTCTTTTAACTTTTTCTCCTCGGGATTGATTTTTTTTATTCTGATTCTGATTCTGATTTTTTTTTTTTTTTTGTCTGGGGTTTCTTTTTCTTTGTTCATAATGTAGCAAATCTTGCGTAAAGATGACGAAGACGCGATTGGATACAATTCGGAAGAAGAAGAATGCGGTTCTCAAGTATTTGAAGAACGACATAGTTGAACTTCTCAAGAGTCGTCTTGATTACAATGCTTTCAACAGGGTTAGTTCATCTTCTTGACGGATTCTGTTCTTAAGCTGGGTTCTAGATTCTTGATCTCTGGTTAATTTCGTCTTTATTGGCTGCAGTCCTCTTCTGTGGTTTCGCTTTATGTGTTACTTTTAGATACCACTCAACAGAGCTTTTGTCCTCCTTTACACATTCTTCTTTTACCTTGAGTTATTGTCAAAGAGAAATTTTGGCTTATCTTCTCTGGTTTTGGTCGCTAATCTATTTCTGTTACTGGCGAAATAGGCTGAAGGGTTTCTTGTTGAGCGAAATGTCTTGAGATGTTATGAACTGATTGATGAGTTTTGTGGCACAATCTCCAATCAAATTCCTGTCCTAAATAAGGAGAGGTAGATAGATATACCATGGATTTTTTTTCATTACATATCTCTTTCTCAGGCTTGAACCTGTTGAGATAGATTTAAATGTTGTTCCCATGTTTTTGTTTCTCTTCAGTGAATGTCCAGATGAATGCAAAGAAGCGGTCGCAACGTTAATTTACGCCGCAGCGAGATTCGCTGACTTGCCTGAATTGCGAGAGCTTCGAAATCTCTTTACTGAGAAATATGGAAGTTCCTTCGGCTCATTTACTAATAAAGAGGTAGTTCTTAGTAGCTCTTATCCATGAATGAAAACTCAACTGTCTTGCTTGTGGGAGTTTCTTCCTTTTTATCATCCTTAAAAGACTGTTTGCTGTTCTTGCTTTTCGTTTTAGTTTATCGAAAAGTCGAGGGCAACAACTCAAACTAAAGAGATGAAGATTCAGCTGCTCCAAGAGATAGCACAGGAAACAGCCATTGATTGGAATTCCAAGGCTCTTGAACAGCAGCTGTATACTCCTCCTCCCCAAAACGAAGTAAGTTTGTTTGCATCTAATTTTCATCTGTTTTTCAATTGAGTAAGTGAATGCTAAAGTAGTTCGAGTGAATTTATGTTTTGTGTCGATAATCTCTCTTCATTCTATTGATGGTTGTGTCTACTGCAATGGTTATTCTTTTCACAGCTTGATGGTGAACGGAGTGGAGCCACCAAAAGAAACAAAACAAAGGTTGTTTCCGTTCCAGTGTATGAAAGAAAGGCAAACTCACCGAGAAACAAGAACAATAGTGATAATGAATCCATCTTTGATAGTAGAAGTGAAGGCAACACAACCGAAACCTCGACAGGAGATAGTACCGATCAAGATATTCATAAAGGCGTTTCGGAGGATGAAGTAGAAGATCAAAAACCCTTCAACCATAGATTTGTCCAACCTCCCTACCTTAAAACAAAACCTATCAAAACAGAAGCAAATGAAAAGGAACCTCGAAAAGTAACTATCGAAAACGATGAAAGTAATAATCCAAAATCACCCACAGAAGAGAAGCCGAAACCGAGGTCCGTGAGGAGAAGAAATGTGAAACCACAGCCTGCAAGAGACATCAACATTGACGACGTTGGGAGTTCCACAATCGATGTAACAACAAAAATTAGCTCAAGTCGAAACAAAGGTAAAGAAACCATGATAGGGGAAGAGAAAGGTGCAAGGGATGATGAAGAAAGGGTATTAGATGGGCTTTTAATGCAATATAGTAAGAAGAAAACAAATCAAGAATCAAAAAGTAGAGGAAAAAGCAACCTTAAACCACAAAGACAGCAAGAGAAAGACAATATTGAACATCAAAGGCCCACAACCAGAGCAGTATCACTTCCCACAGACCAAAATGAGCCAACAAAAAAGCATACCAGAACAAATTCCTTTGTTCATCCTAAGCTCCCAGAATACGACCAACTCGCAGCTCGGATTGCAGCACTGAAAGAGAAGTAAAAGACATCGATCCGTAACTGTGACCGTGTTACGTCGACATGTTAAAAAGGCTCCATGCATTCTATATGGTAAAAAGAAGATACTTTACTTGGAAACTAGACCAATTGCACACTTGCAATTCTATAGGAAAATTCTAATCCTTTCTGACAACGTACTCTGCTAAAGGTTTGGAGTATTATCTTAATACTTTGATATATCATATCATATCATATATATTGTGTAGCTCTTAAGAGTATATAAATTCACGTTGAAGTGTGATATTTTGAGTGCCATTTTTACTCATATGATGATAAAGTTTAGGGTTTGTCGGAGAATCAACGACAAGAAAGAAGACAGCCTTGGGAATCGTTAATTAATCCAAAGAATTT

mRNA sequence

TCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCCTCACAGCTGGGATCATCCATTACCTTTTTATCGGACTTCTTCTTCTTCTTCTTCTTCATTCACATTTCTTCTTTTCTTTTACTGTTTTCTCATTTGGGGGTTTCTTTTTTGTCCCTTTTTTTTGGGGATACTTTTTGTTTTTGTCGTTAAGCTTCTCTGTTTTCTCTTGCTCTGTCGTTTCTGTGTTTCGTTTATGTTATTCCATTTGGTTGATTATTCATCGTCCTTTTTTATTATTTCTTGAAGATCCTTCTATGGTGGATGTGGGACTGTTCTATTTTCATTCCTTCTTATAACTGTCCTTCATTCGGGGTTGTTCTTTCTTGGCGTCTGGTTTGTTTTTTTGATCTTTAGTTTCTTGTGCTTATGGGTTTCTTGGTTTTAGATTCTTAGTACATTTTTACCGATTTCAATTTGGGAAATTCTTGTTTGCCTTTGTTGTTCGGGATTTTGGATTCCCCATTTCCCTGATTCTTCGGTTTTCTTCCTTATTTTAACCCCCTAAAATTCCGTTTCCTGTCAATTTTGCTTTGCGATCACAATGTTCGACATGTTCTTGAAGCCTAAGTTCTATACAAAATGCAAATCTTGCGTAAAGATGACGAAGACGCGATTGGATACAATTCGGAAGAAGAAGAATGCGGTTCTCAAGTATTTGAAGAACGACATAGTTGAACTTCTCAAGAGTCGTCTTGATTACAATGCTTTCAACAGGGCTGAAGGGTTTCTTGTTGAGCGAAATGTCTTGAGATGTTATGAACTGATTGATGAGTTTTGTGGCACAATCTCCAATCAAATTCCTGTCCTAAATAAGGAGAGTGAATGTCCAGATGAATGCAAAGAAGCGGTCGCAACGTTAATTTACGCCGCAGCGAGATTCGCTGACTTGCCTGAATTGCGAGAGCTTCGAAATCTCTTTACTGAGAAATATGGAAGTTCCTTCGGCTCATTTACTAATAAAGAGTTTATCGAAAAGTCGAGGGCAACAACTCAAACTAAAGAGATGAAGATTCAGCTGCTCCAAGAGATAGCACAGGAAACAGCCATTGATTGGAATTCCAAGGCTCTTGAACAGCAGCTGTATACTCCTCCTCCCCAAAACGAACTTGATGGTGAACGGAGTGGAGCCACCAAAAGAAACAAAACAAAGGTTGTTTCCGTTCCAGTGTATGAAAGAAAGGCAAACTCACCGAGAAACAAGAACAATAGTGATAATGAATCCATCTTTGATAGTAGAAGTGAAGGCAACACAACCGAAACCTCGACAGGAGATAGTACCGATCAAGATATTCATAAAGGCGTTTCGGAGGATGAAGTAGAAGATCAAAAACCCTTCAACCATAGATTTGTCCAACCTCCCTACCTTAAAACAAAACCTATCAAAACAGAAGCAAATGAAAAGGAACCTCGAAAAGTAACTATCGAAAACGATGAAAGTAATAATCCAAAATCACCCACAGAAGAGAAGCCGAAACCGAGGTCCGTGAGGAGAAGAAATGTGAAACCACAGCCTGCAAGAGACATCAACATTGACGACGTTGGGAGTTCCACAATCGATGTAACAACAAAAATTAGCTCAAGTCGAAACAAAGGTAAAGAAACCATGATAGGGGAAGAGAAAGGTGCAAGGGATGATGAAGAAAGGGTATTAGATGGGCTTTTAATGCAATATAGTAAGAAGAAAACAAATCAAGAATCAAAAAGTAGAGGAAAAAGCAACCTTAAACCACAAAGACAGCAAGAGAAAGACAATATTGAACATCAAAGGCCCACAACCAGAGCAGTATCACTTCCCACAGACCAAAATGAGCCAACAAAAAAGCATACCAGAACAAATTCCTTTGTTCATCCTAAGCTCCCAGAATACGACCAACTCGCAGCTCGGATTGCAGCACTGAAAGAGAAGTAAAAGACATCGATCCGTAACTGTGACCGTGTTACGTCGACATGTTAAAAAGGCTCCATGCATTCTATATGGTAAAAAGAAGATACTTTACTTGGAAACTAGACCAATTGCACACTTGCAATTCTATAGGAAAATTCTAATCCTTTCTGACAACGTACTCTGCTAAAGGTTTGGAGTATTATCTTAATACTTTGATATATCATATCATATCATATATATTGTGTAGCTCTTAAGAGTATATAAATTCACGTTGAAGTGTGATATTTTGAGTGCCATTTTTACTCATATGATGATAAAGTTTAGGGTTTGTCGGAGAATCAACGACAAGAAAGAAGACAGCCTTGGGAATCGTTAATTAATCCAAAGAATTT

Coding sequence (CDS)

ATGTTCGACATGTTCTTGAAGCCTAAGTTCTATACAAAATGCAAATCTTGCGTAAAGATGACGAAGACGCGATTGGATACAATTCGGAAGAAGAAGAATGCGGTTCTCAAGTATTTGAAGAACGACATAGTTGAACTTCTCAAGAGTCGTCTTGATTACAATGCTTTCAACAGGGCTGAAGGGTTTCTTGTTGAGCGAAATGTCTTGAGATGTTATGAACTGATTGATGAGTTTTGTGGCACAATCTCCAATCAAATTCCTGTCCTAAATAAGGAGAGTGAATGTCCAGATGAATGCAAAGAAGCGGTCGCAACGTTAATTTACGCCGCAGCGAGATTCGCTGACTTGCCTGAATTGCGAGAGCTTCGAAATCTCTTTACTGAGAAATATGGAAGTTCCTTCGGCTCATTTACTAATAAAGAGTTTATCGAAAAGTCGAGGGCAACAACTCAAACTAAAGAGATGAAGATTCAGCTGCTCCAAGAGATAGCACAGGAAACAGCCATTGATTGGAATTCCAAGGCTCTTGAACAGCAGCTGTATACTCCTCCTCCCCAAAACGAACTTGATGGTGAACGGAGTGGAGCCACCAAAAGAAACAAAACAAAGGTTGTTTCCGTTCCAGTGTATGAAAGAAAGGCAAACTCACCGAGAAACAAGAACAATAGTGATAATGAATCCATCTTTGATAGTAGAAGTGAAGGCAACACAACCGAAACCTCGACAGGAGATAGTACCGATCAAGATATTCATAAAGGCGTTTCGGAGGATGAAGTAGAAGATCAAAAACCCTTCAACCATAGATTTGTCCAACCTCCCTACCTTAAAACAAAACCTATCAAAACAGAAGCAAATGAAAAGGAACCTCGAAAAGTAACTATCGAAAACGATGAAAGTAATAATCCAAAATCACCCACAGAAGAGAAGCCGAAACCGAGGTCCGTGAGGAGAAGAAATGTGAAACCACAGCCTGCAAGAGACATCAACATTGACGACGTTGGGAGTTCCACAATCGATGTAACAACAAAAATTAGCTCAAGTCGAAACAAAGGTAAAGAAACCATGATAGGGGAAGAGAAAGGTGCAAGGGATGATGAAGAAAGGGTATTAGATGGGCTTTTAATGCAATATAGTAAGAAGAAAACAAATCAAGAATCAAAAAGTAGAGGAAAAAGCAACCTTAAACCACAAAGACAGCAAGAGAAAGACAATATTGAACATCAAAGGCCCACAACCAGAGCAGTATCACTTCCCACAGACCAAAATGAGCCAACAAAAAAGCATACCAGAACAAATTCCTTTGTTCATCCTAAGCTCCCAGAATACGACCAACTCGCAGCTCGGATTGCAGCACTGAAAGAGAAGTAA

Protein sequence

MFDMFLKPKFYTKCKSCVKMTKTRLDTIRKKKNAVLKYLKNDIVELLKSRLDYNAFNRAEGFLVERNVLRCYELIDEFCGTISNQIPVLNKESECPDECKEAVATLIYAAARFADLPELRELRNLFTEKYGSSFGSFTNKEFIEKSRATTQTKEMKIQLLQEIAQETAIDWNSKALEQQLYTPPPQNELDGERSGATKRNKTKVVSVPVYERKANSPRNKNNSDNESIFDSRSEGNTTETSTGDSTDQDIHKGVSEDEVEDQKPFNHRFVQPPYLKTKPIKTEANEKEPRKVTIENDESNNPKSPTEEKPKPRSVRRRNVKPQPARDINIDDVGSSTIDVTTKISSSRNKGKETMIGEEKGARDDEERVLDGLLMQYSKKKTNQESKSRGKSNLKPQRQQEKDNIEHQRPTTRAVSLPTDQNEPTKKHTRTNSFVHPKLPEYDQLAARIAALKEK
Homology
BLAST of Pay0001623 vs. ExPASy Swiss-Prot
Match: Q54I39 (IST1-like protein OS=Dictyostelium discoideum OX=44689 GN=DDB_G0289029 PE=3 SV=1)

HSP 1 Score: 83.2 bits (204), Expect = 8.4e-15
Identity = 49/182 (26.92%), Postives = 93/182 (51.10%), Query Frame = 0

Query: 13  KCKSCVKMTKTRLDTIRKKKNAVLKYLKNDIVELLKSRLDYNAFNRAEGFLVERNVLRCY 72
           K K  +K+  +R+  ++ KK  +++  K ++ ELL+ + + +A  R E  + +  ++ C+
Sbjct: 12  KLKVQLKLAVSRIQILKNKKANIVRDEKRNVAELLRKKNEESARIRVETIIRDEYLIECF 71

Query: 73  ELIDEFCGTISNQIPVLNKESECPDECKEAVATLIYAAARFADLPELRELRNLFTEKYGS 132
           ++I+  C  +  +I ++N  +E P E KE++ TL+Y++ R   +PEL +++N    KYG 
Sbjct: 72  QIIEVLCELLHARINLINATTEMPLEMKESIFTLVYSSQRI-QIPELEQIKNQLKAKYGK 131

Query: 133 SF--------GSFTNKEFIEKSRATTQTKEMKIQLLQEIAQETAIDWNSKALEQQLYTPP 187
                      +  N + + K    T    +  Q L EIA++  +DW         Y PP
Sbjct: 132 GLENEANCHCSTHVNPKIVHKLSYATPDPSIIFQTLSEIAEKFNVDWCGSD-----YPPP 187

BLAST of Pay0001623 vs. ExPASy TrEMBL
Match: A0A1S3CGT5 (uncharacterized protein LOC103500577 OS=Cucumis melo OX=3656 GN=LOC103500577 PE=3 SV=1)

HSP 1 Score: 866.3 bits (2237), Expect = 5.7e-248
Identity = 454/455 (99.78%), Postives = 454/455 (99.78%), Query Frame = 0

Query: 1   MFDMFLKPKFYTKCKSCVKMTKTRLDTIRKKKNAVLKYLKNDIVELLKSRLDYNAFNRAE 60
           MFDMFLKPKFYTKCKSCVKMTKTRLDTIRKKKNAVLKYLKNDIVELLKSRLDYNAFNRAE
Sbjct: 1   MFDMFLKPKFYTKCKSCVKMTKTRLDTIRKKKNAVLKYLKNDIVELLKSRLDYNAFNRAE 60

Query: 61  GFLVERNVLRCYELIDEFCGTISNQIPVLNKESECPDECKEAVATLIYAAARFADLPELR 120
           GFLVERNVLRCYELIDEFCGTISNQIPVLNKESECPDECKEAVATLIYAAARFADLPELR
Sbjct: 61  GFLVERNVLRCYELIDEFCGTISNQIPVLNKESECPDECKEAVATLIYAAARFADLPELR 120

Query: 121 ELRNLFTEKYGSSFGSFTNKEFIEKSRATTQTKEMKIQLLQEIAQETAIDWNSKALEQQL 180
           ELRNLFTEKYGSSFGSFTNKEFIEKSRATTQTKEMKIQLLQEIAQETAIDWNSKALEQQL
Sbjct: 121 ELRNLFTEKYGSSFGSFTNKEFIEKSRATTQTKEMKIQLLQEIAQETAIDWNSKALEQQL 180

Query: 181 YTPPPQNELDGERSGATKRNKTKVVSVPVYERKANSPRNKNNSDNESIFDSRSEGNTTET 240
           YTPPPQNELDGERSGATKRNKTKVVSVPVYERKANSPRNKNNSDNESIFDSRSEGNTTET
Sbjct: 181 YTPPPQNELDGERSGATKRNKTKVVSVPVYERKANSPRNKNNSDNESIFDSRSEGNTTET 240

Query: 241 STGDSTDQDIHKGVSEDEVEDQKPFNHRFVQPPYLKTKPIKTEANEKEPRKVTIENDESN 300
           STGDSTDQDIHKGVSEDEVEDQKPFNHRFVQPPYLKTKPIKTEANEKEPRKVTIENDESN
Sbjct: 241 STGDSTDQDIHKGVSEDEVEDQKPFNHRFVQPPYLKTKPIKTEANEKEPRKVTIENDESN 300

Query: 301 NPKSPTEEKPKPRSVRRRNVKPQPARDINIDDVGSSTIDVTTKISSSRNKGKETMIGEEK 360
           NPKSPTEEKPKPRSVRRRNVKPQPARDINIDDVGSSTIDVTTKISSSRNKGKETMIGEEK
Sbjct: 301 NPKSPTEEKPKPRSVRRRNVKPQPARDINIDDVGSSTIDVTTKISSSRNKGKETMIGEEK 360

Query: 361 GARDDEERVLDGLLMQYSKKKTNQESKSRGKSNLKPQRQQEKDNIEHQRPTTRAVSLPTD 420
           GARDDEERVLDGLLMQYSKKKTNQESKSRGKSNLKPQRQQEKDNIEHQRPTTRAVSLPTD
Sbjct: 361 GARDDEERVLDGLLMQYSKKKTNQESKSRGKSNLKPQRQQEKDNIEHQRPTTRAVSLPTD 420

Query: 421 QNEPTKKHTRTNSFVHPKLPEYDQLAARIAALKEK 456
           QNE TKKHTRTNSFVHPKLPEYDQLAARIAALKEK
Sbjct: 421 QNESTKKHTRTNSFVHPKLPEYDQLAARIAALKEK 455

BLAST of Pay0001623 vs. ExPASy TrEMBL
Match: A0A5D3BX00 (Putative Regulator of Vps4 activity in the MVB pathway protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold347G002360 PE=3 SV=1)

HSP 1 Score: 826.2 bits (2133), Expect = 6.5e-236
Identity = 435/436 (99.77%), Postives = 435/436 (99.77%), Query Frame = 0

Query: 20  MTKTRLDTIRKKKNAVLKYLKNDIVELLKSRLDYNAFNRAEGFLVERNVLRCYELIDEFC 79
           MTKTRLDTIRKKKNAVLKYLKNDIVELLKSRLDYNAFNRAEGFLVERNVLRCYELIDEFC
Sbjct: 1   MTKTRLDTIRKKKNAVLKYLKNDIVELLKSRLDYNAFNRAEGFLVERNVLRCYELIDEFC 60

Query: 80  GTISNQIPVLNKESECPDECKEAVATLIYAAARFADLPELRELRNLFTEKYGSSFGSFTN 139
           GTISNQIPVLNKESECPDECKEAVATLIYAAARFADLPELRELRNLFTEKYGSSFGSFTN
Sbjct: 61  GTISNQIPVLNKESECPDECKEAVATLIYAAARFADLPELRELRNLFTEKYGSSFGSFTN 120

Query: 140 KEFIEKSRATTQTKEMKIQLLQEIAQETAIDWNSKALEQQLYTPPPQNELDGERSGATKR 199
           KEFIEKSRATTQTKEMKIQLLQEIAQETAIDWNSKALEQQLYTPPPQNELDGERSGATKR
Sbjct: 121 KEFIEKSRATTQTKEMKIQLLQEIAQETAIDWNSKALEQQLYTPPPQNELDGERSGATKR 180

Query: 200 NKTKVVSVPVYERKANSPRNKNNSDNESIFDSRSEGNTTETSTGDSTDQDIHKGVSEDEV 259
           NKTKVVSVPVYERKANSPRNKNNSDNESIFDSRSEGNTTETSTGDSTDQDIHKGVSEDEV
Sbjct: 181 NKTKVVSVPVYERKANSPRNKNNSDNESIFDSRSEGNTTETSTGDSTDQDIHKGVSEDEV 240

Query: 260 EDQKPFNHRFVQPPYLKTKPIKTEANEKEPRKVTIENDESNNPKSPTEEKPKPRSVRRRN 319
           EDQKPFNHRFVQPPYLKTKPIKTEANEKEPRKVTIENDESNNPKSPTEEKPKPRSVRRRN
Sbjct: 241 EDQKPFNHRFVQPPYLKTKPIKTEANEKEPRKVTIENDESNNPKSPTEEKPKPRSVRRRN 300

Query: 320 VKPQPARDINIDDVGSSTIDVTTKISSSRNKGKETMIGEEKGARDDEERVLDGLLMQYSK 379
           VKPQPARDINIDDVGSSTIDVTTKISSSRNKGKETMIGEEKGARDDEERVLDGLLMQYSK
Sbjct: 301 VKPQPARDINIDDVGSSTIDVTTKISSSRNKGKETMIGEEKGARDDEERVLDGLLMQYSK 360

Query: 380 KKTNQESKSRGKSNLKPQRQQEKDNIEHQRPTTRAVSLPTDQNEPTKKHTRTNSFVHPKL 439
           KKTNQESKSRGKSNLKPQRQQEKDNIEHQRPTTRAVSLPTDQNE TKKHTRTNSFVHPKL
Sbjct: 361 KKTNQESKSRGKSNLKPQRQQEKDNIEHQRPTTRAVSLPTDQNESTKKHTRTNSFVHPKL 420

Query: 440 PEYDQLAARIAALKEK 456
           PEYDQLAARIAALKEK
Sbjct: 421 PEYDQLAARIAALKEK 436

BLAST of Pay0001623 vs. ExPASy TrEMBL
Match: A0A0A0K926 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G446760 PE=3 SV=1)

HSP 1 Score: 817.4 bits (2110), Expect = 3.0e-233
Identity = 434/456 (95.18%), Postives = 442/456 (96.93%), Query Frame = 0

Query: 1   MFDMFLKPKFYTKCKSCVKMTKTRLDTIRKKKNAVLKYLKNDIVELLKSRLDYNAFNRAE 60
           MFDMFLKPKFYTKCKSCVKMTKTRLDT RKKKNAVLKYLKNDIVELLKSRLDYNA+NRAE
Sbjct: 1   MFDMFLKPKFYTKCKSCVKMTKTRLDTTRKKKNAVLKYLKNDIVELLKSRLDYNAYNRAE 60

Query: 61  GFLVERNVLRCYELIDEFCGTISNQIPVLNKESECPDECKEAVATLIYAAARFADLPELR 120
           GFLVERNVLRCYELIDEFCGTISNQIPVLNKESECPDECKE+VATLIYAAARFADLPELR
Sbjct: 61  GFLVERNVLRCYELIDEFCGTISNQIPVLNKESECPDECKESVATLIYAAARFADLPELR 120

Query: 121 ELRNLFTEKYGSSFGSFTNKEFIEKSRATTQTKEMKIQLLQEIAQETAIDWNSKALEQQL 180
           ELRNLFTEKYGSSFGSFTNKEFIEKSR TTQTKEMKIQLLQEIAQETAIDWNSKALEQQL
Sbjct: 121 ELRNLFTEKYGSSFGSFTNKEFIEKSRTTTQTKEMKIQLLQEIAQETAIDWNSKALEQQL 180

Query: 181 YTPPPQNELDGERSGATKRNKTKVVSVPVYERKANSPRNKNNSDNESIFDSRSEGNTTET 240
           YTPPP+NELDGERSGATKRNKTKVVSVPVYE+KANSPRNKNNSDNESIFDSRSEGNTTET
Sbjct: 181 YTPPPENELDGERSGATKRNKTKVVSVPVYEKKANSPRNKNNSDNESIFDSRSEGNTTET 240

Query: 241 STGDSTDQDIHKGVSEDEVEDQKPFNHRFVQPPYLKTKPIKTEANEKE-PRKVTIENDES 300
           STGDSTDQD+HKGVS DEV DQKPFN RFVQPPYLKTKPIKTEAN +E PRKVTIENDES
Sbjct: 241 STGDSTDQDVHKGVSGDEV-DQKPFNRRFVQPPYLKTKPIKTEANAEELPRKVTIENDES 300

Query: 301 NNPKSPTEEKPKPRSVRRRNVKPQPARDINIDDVGSSTIDVTTKISSSRNKGKETMIGEE 360
            NPKSPTEEKPKPRSVRRR VKPQPARDINIDDVGSST+DVT KISS RNKGKETMIGEE
Sbjct: 301 -NPKSPTEEKPKPRSVRRRIVKPQPARDINIDDVGSSTVDVTKKISSIRNKGKETMIGEE 360

Query: 361 KGARDDEERVLDGLLMQYSKKKTNQESKSRGKSNLKPQRQQEKDNIEHQRPTTRAVSLPT 420
           KGARDDEERVLDGLLMQYSKKKTNQESKSRGKSNLKPQRQQEKDNIEHQRPT+RAVS P 
Sbjct: 361 KGARDDEERVLDGLLMQYSKKKTNQESKSRGKSNLKPQRQQEKDNIEHQRPTSRAVSFPP 420

Query: 421 DQNEPTKKHTRTNSFVHPKLPEYDQLAARIAALKEK 456
           DQNEP KKHTRTNSFVHPKLPEYDQLAARIAALKEK
Sbjct: 421 DQNEPMKKHTRTNSFVHPKLPEYDQLAARIAALKEK 454

BLAST of Pay0001623 vs. ExPASy TrEMBL
Match: A0A6J1GMM1 (uncharacterized protein LOC111455825 OS=Cucurbita moschata OX=3662 GN=LOC111455825 PE=3 SV=1)

HSP 1 Score: 609.8 bits (1571), Expect = 9.5e-171
Identity = 340/460 (73.91%), Postives = 377/460 (81.96%), Query Frame = 0

Query: 1   MFDMFLKPKFYTKCKSCVKMTKTRLDTIRKKKNAVLKYLKNDIVELLKSRLDYNAFNRAE 60
           MF MF KPKFYTKCKSCVKMTKTRLDTIRKKK  VLK+LKNDIVELLKSRLDYNA+NRAE
Sbjct: 1   MFRMFFKPKFYTKCKSCVKMTKTRLDTIRKKKKVVLKFLKNDIVELLKSRLDYNAYNRAE 60

Query: 61  GFLVERNVLRCYELIDEFCGTISNQIPVLNKESECPDECKEAVATLIYAAARFADLPELR 120
           GFLVE+NVLRCY+LIDEFCGTI   I VLNKESECPDECKEAVA+LIYAAARF+DLPELR
Sbjct: 61  GFLVEQNVLRCYDLIDEFCGTIFKHISVLNKESECPDECKEAVASLIYAAARFSDLPELR 120

Query: 121 ELRNLFTEKYGSSFGSFTNKEFIEKSRATTQTKEMKIQLLQEIAQETAIDWNSKALEQQL 180
            LR+LFTE+YG+SFGSFTNKE +EK RA  QTKE K+QLLQEIAQE+AIDWNSKALEQQL
Sbjct: 121 VLRSLFTERYGTSFGSFTNKELVEKLRAVAQTKETKLQLLQEIAQESAIDWNSKALEQQL 180

Query: 181 YTPPPQNELDGERSGATKRNKTKVVSVPVYERKANSPRNKNNSDNESIFDSRSEGNTTET 240
           Y  PPQNELD ERS +T R+K+K+VSVPVY R  NSPR K+NSD++SIFDSRSEGNTTET
Sbjct: 181 YV-PPQNELDCERSTSTCRSKSKIVSVPVYGRTTNSPRKKDNSDDDSIFDSRSEGNTTET 240

Query: 241 STGD-STDQDIHKG-VSEDEVEDQKPFNHRFVQPPYLKTKPIKTEANEKEPRKVTIENDE 300
           S GD STDQD+HKG VSEDEVEDQKPF  RF+ PPYLKTKP K E N +EP+  T EN  
Sbjct: 241 SMGDSSTDQDVHKGVVSEDEVEDQKPFYLRFITPPYLKTKPTKKEENVEEPQNDTEEN-- 300

Query: 301 SNNPKSPTEEKPKPRSVRRRNVKPQPARDINIDDVGSSTIDVTTKISSSRNKGKETMIGE 360
            N+PK+  EEKPKPRSVRRR+ KPQPAR INIDD G S  D   KISSSRNKGKE M GE
Sbjct: 301 RNDPKTAIEEKPKPRSVRRRHAKPQPARSINIDDAGGSAADGAGKISSSRNKGKEAMTGE 360

Query: 361 EKGARDDEERVLDGLLMQYSKKKTNQESKSRGKSNLKPQRQQEKDNIEHQRPTTRAVSLP 420
           EKG  DDEER+LDGLL  YSKKKT QE     K N +PQR +     +   P T+++SLP
Sbjct: 361 EKGVEDDEERMLDGLLKLYSKKKTRQED----KDNAEPQRPKNTRTEDAYVP-TKSLSLP 420

Query: 421 ---TDQNEPTKKHTRTNSFVHPKLPEYDQLAARIAALKEK 456
              T+  EP KKH R NS+VHPKLP+YDQLAAR A+LKEK
Sbjct: 421 PPRTEPIEPVKKHARANSYVHPKLPDYDQLAARFASLKEK 452

BLAST of Pay0001623 vs. ExPASy TrEMBL
Match: A0A6J1JV69 (uncharacterized protein LOC111488629 OS=Cucurbita maxima OX=3661 GN=LOC111488629 PE=3 SV=1)

HSP 1 Score: 606.7 bits (1563), Expect = 8.1e-170
Identity = 337/460 (73.26%), Postives = 376/460 (81.74%), Query Frame = 0

Query: 1   MFDMFLKPKFYTKCKSCVKMTKTRLDTIRKKKNAVLKYLKNDIVELLKSRLDYNAFNRAE 60
           MF MF KPKFYTKCKSCVKMTKTRLDTIRKKK  VLK+LKNDIVELLKSRLDYNA+NRAE
Sbjct: 1   MFRMFFKPKFYTKCKSCVKMTKTRLDTIRKKKKVVLKFLKNDIVELLKSRLDYNAYNRAE 60

Query: 61  GFLVERNVLRCYELIDEFCGTISNQIPVLNKESECPDECKEAVATLIYAAARFADLPELR 120
           GFLVE+NVLRCY+LIDEFCGTI N I VLNKESECPDECKEAVA+LIYAAARF+DLPELR
Sbjct: 61  GFLVEQNVLRCYDLIDEFCGTIFNHISVLNKESECPDECKEAVASLIYAAARFSDLPELR 120

Query: 121 ELRNLFTEKYGSSFGSFTNKEFIEKSRATTQTKEMKIQLLQEIAQETAIDWNSKALEQQL 180
            LR+LFTE+YG+SFGSFTNKE +EK RA  QTKE K+QLLQEIAQE+AIDWNSKALEQQL
Sbjct: 121 VLRSLFTERYGTSFGSFTNKELVEKLRAVAQTKETKLQLLQEIAQESAIDWNSKALEQQL 180

Query: 181 YTPPPQNELDGERSGATKRNKTKVVSVPVYERKANSPRNKNNSDNESIFDSRSEGNTTET 240
           Y  PPQNEL  ERS +T R+K+K+VSVPVY R  NSP+ K+NSD++SIFDSRSEGNTTET
Sbjct: 181 YV-PPQNELVRERSTSTNRSKSKIVSVPVYGRTTNSPKKKDNSDDDSIFDSRSEGNTTET 240

Query: 241 STGD-STDQDIHKG-VSEDEVEDQKPFNHRFVQPPYLKTKPIKTEANEKEPRKVTIENDE 300
           S GD STDQD+HKG VSEDEVEDQKPF  RF+ PPYLKTKP K E N +EP+ VT   ++
Sbjct: 241 SMGDSSTDQDVHKGIVSEDEVEDQKPFYLRFITPPYLKTKPTKKEENVEEPQNVT--EEK 300

Query: 301 SNNPKSPTEEKPKPRSVRRRNVKPQPARDINIDDVGSSTIDVTTKISSSRNKGKETMIGE 360
            NNPK+ TEEKPKPRSVRRR+ KPQPAR INIDD G S  D   KISS +NKGKE M GE
Sbjct: 301 RNNPKTTTEEKPKPRSVRRRHAKPQPARSINIDDSGGSAADGAGKISSGQNKGKEAMTGE 360

Query: 361 EKGARDDEERVLDGLLMQYSKKKTNQESKSRGKSNLKPQRQQEKDNIEHQRPTTRAVSLP 420
           EKG  DDEER+LDGLL  YSKK T QE     K N +PQR +     +   P  +++SLP
Sbjct: 361 EKGEEDDEERMLDGLLKIYSKKNTRQED----KDNAEPQRPKNTRTEDAYVP-AKSLSLP 420

Query: 421 TDQN---EPTKKHTRTNSFVHPKLPEYDQLAARIAALKEK 456
           T +    EP KKH R NS+VHPKLP+YDQLAAR AALKEK
Sbjct: 421 TARTEPIEPVKKHARANSYVHPKLPDYDQLAARFAALKEK 452

BLAST of Pay0001623 vs. NCBI nr
Match: XP_008462153.1 (PREDICTED: uncharacterized protein LOC103500577 [Cucumis melo])

HSP 1 Score: 866.3 bits (2237), Expect = 1.2e-247
Identity = 454/455 (99.78%), Postives = 454/455 (99.78%), Query Frame = 0

Query: 1   MFDMFLKPKFYTKCKSCVKMTKTRLDTIRKKKNAVLKYLKNDIVELLKSRLDYNAFNRAE 60
           MFDMFLKPKFYTKCKSCVKMTKTRLDTIRKKKNAVLKYLKNDIVELLKSRLDYNAFNRAE
Sbjct: 1   MFDMFLKPKFYTKCKSCVKMTKTRLDTIRKKKNAVLKYLKNDIVELLKSRLDYNAFNRAE 60

Query: 61  GFLVERNVLRCYELIDEFCGTISNQIPVLNKESECPDECKEAVATLIYAAARFADLPELR 120
           GFLVERNVLRCYELIDEFCGTISNQIPVLNKESECPDECKEAVATLIYAAARFADLPELR
Sbjct: 61  GFLVERNVLRCYELIDEFCGTISNQIPVLNKESECPDECKEAVATLIYAAARFADLPELR 120

Query: 121 ELRNLFTEKYGSSFGSFTNKEFIEKSRATTQTKEMKIQLLQEIAQETAIDWNSKALEQQL 180
           ELRNLFTEKYGSSFGSFTNKEFIEKSRATTQTKEMKIQLLQEIAQETAIDWNSKALEQQL
Sbjct: 121 ELRNLFTEKYGSSFGSFTNKEFIEKSRATTQTKEMKIQLLQEIAQETAIDWNSKALEQQL 180

Query: 181 YTPPPQNELDGERSGATKRNKTKVVSVPVYERKANSPRNKNNSDNESIFDSRSEGNTTET 240
           YTPPPQNELDGERSGATKRNKTKVVSVPVYERKANSPRNKNNSDNESIFDSRSEGNTTET
Sbjct: 181 YTPPPQNELDGERSGATKRNKTKVVSVPVYERKANSPRNKNNSDNESIFDSRSEGNTTET 240

Query: 241 STGDSTDQDIHKGVSEDEVEDQKPFNHRFVQPPYLKTKPIKTEANEKEPRKVTIENDESN 300
           STGDSTDQDIHKGVSEDEVEDQKPFNHRFVQPPYLKTKPIKTEANEKEPRKVTIENDESN
Sbjct: 241 STGDSTDQDIHKGVSEDEVEDQKPFNHRFVQPPYLKTKPIKTEANEKEPRKVTIENDESN 300

Query: 301 NPKSPTEEKPKPRSVRRRNVKPQPARDINIDDVGSSTIDVTTKISSSRNKGKETMIGEEK 360
           NPKSPTEEKPKPRSVRRRNVKPQPARDINIDDVGSSTIDVTTKISSSRNKGKETMIGEEK
Sbjct: 301 NPKSPTEEKPKPRSVRRRNVKPQPARDINIDDVGSSTIDVTTKISSSRNKGKETMIGEEK 360

Query: 361 GARDDEERVLDGLLMQYSKKKTNQESKSRGKSNLKPQRQQEKDNIEHQRPTTRAVSLPTD 420
           GARDDEERVLDGLLMQYSKKKTNQESKSRGKSNLKPQRQQEKDNIEHQRPTTRAVSLPTD
Sbjct: 361 GARDDEERVLDGLLMQYSKKKTNQESKSRGKSNLKPQRQQEKDNIEHQRPTTRAVSLPTD 420

Query: 421 QNEPTKKHTRTNSFVHPKLPEYDQLAARIAALKEK 456
           QNE TKKHTRTNSFVHPKLPEYDQLAARIAALKEK
Sbjct: 421 QNESTKKHTRTNSFVHPKLPEYDQLAARIAALKEK 455

BLAST of Pay0001623 vs. NCBI nr
Match: KAA0059291.1 (putative Regulator of Vps4 activity in the MVB pathway protein [Cucumis melo var. makuwa] >TYK04037.1 putative Regulator of Vps4 activity in the MVB pathway protein [Cucumis melo var. makuwa])

HSP 1 Score: 826.2 bits (2133), Expect = 1.3e-235
Identity = 435/436 (99.77%), Postives = 435/436 (99.77%), Query Frame = 0

Query: 20  MTKTRLDTIRKKKNAVLKYLKNDIVELLKSRLDYNAFNRAEGFLVERNVLRCYELIDEFC 79
           MTKTRLDTIRKKKNAVLKYLKNDIVELLKSRLDYNAFNRAEGFLVERNVLRCYELIDEFC
Sbjct: 1   MTKTRLDTIRKKKNAVLKYLKNDIVELLKSRLDYNAFNRAEGFLVERNVLRCYELIDEFC 60

Query: 80  GTISNQIPVLNKESECPDECKEAVATLIYAAARFADLPELRELRNLFTEKYGSSFGSFTN 139
           GTISNQIPVLNKESECPDECKEAVATLIYAAARFADLPELRELRNLFTEKYGSSFGSFTN
Sbjct: 61  GTISNQIPVLNKESECPDECKEAVATLIYAAARFADLPELRELRNLFTEKYGSSFGSFTN 120

Query: 140 KEFIEKSRATTQTKEMKIQLLQEIAQETAIDWNSKALEQQLYTPPPQNELDGERSGATKR 199
           KEFIEKSRATTQTKEMKIQLLQEIAQETAIDWNSKALEQQLYTPPPQNELDGERSGATKR
Sbjct: 121 KEFIEKSRATTQTKEMKIQLLQEIAQETAIDWNSKALEQQLYTPPPQNELDGERSGATKR 180

Query: 200 NKTKVVSVPVYERKANSPRNKNNSDNESIFDSRSEGNTTETSTGDSTDQDIHKGVSEDEV 259
           NKTKVVSVPVYERKANSPRNKNNSDNESIFDSRSEGNTTETSTGDSTDQDIHKGVSEDEV
Sbjct: 181 NKTKVVSVPVYERKANSPRNKNNSDNESIFDSRSEGNTTETSTGDSTDQDIHKGVSEDEV 240

Query: 260 EDQKPFNHRFVQPPYLKTKPIKTEANEKEPRKVTIENDESNNPKSPTEEKPKPRSVRRRN 319
           EDQKPFNHRFVQPPYLKTKPIKTEANEKEPRKVTIENDESNNPKSPTEEKPKPRSVRRRN
Sbjct: 241 EDQKPFNHRFVQPPYLKTKPIKTEANEKEPRKVTIENDESNNPKSPTEEKPKPRSVRRRN 300

Query: 320 VKPQPARDINIDDVGSSTIDVTTKISSSRNKGKETMIGEEKGARDDEERVLDGLLMQYSK 379
           VKPQPARDINIDDVGSSTIDVTTKISSSRNKGKETMIGEEKGARDDEERVLDGLLMQYSK
Sbjct: 301 VKPQPARDINIDDVGSSTIDVTTKISSSRNKGKETMIGEEKGARDDEERVLDGLLMQYSK 360

Query: 380 KKTNQESKSRGKSNLKPQRQQEKDNIEHQRPTTRAVSLPTDQNEPTKKHTRTNSFVHPKL 439
           KKTNQESKSRGKSNLKPQRQQEKDNIEHQRPTTRAVSLPTDQNE TKKHTRTNSFVHPKL
Sbjct: 361 KKTNQESKSRGKSNLKPQRQQEKDNIEHQRPTTRAVSLPTDQNESTKKHTRTNSFVHPKL 420

Query: 440 PEYDQLAARIAALKEK 456
           PEYDQLAARIAALKEK
Sbjct: 421 PEYDQLAARIAALKEK 436

BLAST of Pay0001623 vs. NCBI nr
Match: XP_004141848.1 (uncharacterized protein LOC101220202 [Cucumis sativus] >KGN45359.1 hypothetical protein Csa_015927 [Cucumis sativus])

HSP 1 Score: 817.4 bits (2110), Expect = 6.2e-233
Identity = 434/456 (95.18%), Postives = 442/456 (96.93%), Query Frame = 0

Query: 1   MFDMFLKPKFYTKCKSCVKMTKTRLDTIRKKKNAVLKYLKNDIVELLKSRLDYNAFNRAE 60
           MFDMFLKPKFYTKCKSCVKMTKTRLDT RKKKNAVLKYLKNDIVELLKSRLDYNA+NRAE
Sbjct: 1   MFDMFLKPKFYTKCKSCVKMTKTRLDTTRKKKNAVLKYLKNDIVELLKSRLDYNAYNRAE 60

Query: 61  GFLVERNVLRCYELIDEFCGTISNQIPVLNKESECPDECKEAVATLIYAAARFADLPELR 120
           GFLVERNVLRCYELIDEFCGTISNQIPVLNKESECPDECKE+VATLIYAAARFADLPELR
Sbjct: 61  GFLVERNVLRCYELIDEFCGTISNQIPVLNKESECPDECKESVATLIYAAARFADLPELR 120

Query: 121 ELRNLFTEKYGSSFGSFTNKEFIEKSRATTQTKEMKIQLLQEIAQETAIDWNSKALEQQL 180
           ELRNLFTEKYGSSFGSFTNKEFIEKSR TTQTKEMKIQLLQEIAQETAIDWNSKALEQQL
Sbjct: 121 ELRNLFTEKYGSSFGSFTNKEFIEKSRTTTQTKEMKIQLLQEIAQETAIDWNSKALEQQL 180

Query: 181 YTPPPQNELDGERSGATKRNKTKVVSVPVYERKANSPRNKNNSDNESIFDSRSEGNTTET 240
           YTPPP+NELDGERSGATKRNKTKVVSVPVYE+KANSPRNKNNSDNESIFDSRSEGNTTET
Sbjct: 181 YTPPPENELDGERSGATKRNKTKVVSVPVYEKKANSPRNKNNSDNESIFDSRSEGNTTET 240

Query: 241 STGDSTDQDIHKGVSEDEVEDQKPFNHRFVQPPYLKTKPIKTEANEKE-PRKVTIENDES 300
           STGDSTDQD+HKGVS DEV DQKPFN RFVQPPYLKTKPIKTEAN +E PRKVTIENDES
Sbjct: 241 STGDSTDQDVHKGVSGDEV-DQKPFNRRFVQPPYLKTKPIKTEANAEELPRKVTIENDES 300

Query: 301 NNPKSPTEEKPKPRSVRRRNVKPQPARDINIDDVGSSTIDVTTKISSSRNKGKETMIGEE 360
            NPKSPTEEKPKPRSVRRR VKPQPARDINIDDVGSST+DVT KISS RNKGKETMIGEE
Sbjct: 301 -NPKSPTEEKPKPRSVRRRIVKPQPARDINIDDVGSSTVDVTKKISSIRNKGKETMIGEE 360

Query: 361 KGARDDEERVLDGLLMQYSKKKTNQESKSRGKSNLKPQRQQEKDNIEHQRPTTRAVSLPT 420
           KGARDDEERVLDGLLMQYSKKKTNQESKSRGKSNLKPQRQQEKDNIEHQRPT+RAVS P 
Sbjct: 361 KGARDDEERVLDGLLMQYSKKKTNQESKSRGKSNLKPQRQQEKDNIEHQRPTSRAVSFPP 420

Query: 421 DQNEPTKKHTRTNSFVHPKLPEYDQLAARIAALKEK 456
           DQNEP KKHTRTNSFVHPKLPEYDQLAARIAALKEK
Sbjct: 421 DQNEPMKKHTRTNSFVHPKLPEYDQLAARIAALKEK 454

BLAST of Pay0001623 vs. NCBI nr
Match: XP_038897244.1 (uncharacterized protein LOC120085370 [Benincasa hispida])

HSP 1 Score: 736.5 bits (1900), Expect = 1.4e-208
Identity = 398/468 (85.04%), Postives = 424/468 (90.60%), Query Frame = 0

Query: 1   MFDMFLKPKFYTKCKSCVKMTKTRLDTIRKKKNAVLKYLKNDIVELLKSRLDYNAFNRAE 60
           MFDMFLKPKFYTKCKSCVKMTKTRLD+IRKKK AVLKYLKNDIVELLKSRLDYNA+NRAE
Sbjct: 1   MFDMFLKPKFYTKCKSCVKMTKTRLDSIRKKKKAVLKYLKNDIVELLKSRLDYNAYNRAE 60

Query: 61  GFLVERNVLRCYELIDEFCGTISNQIPVLNKESECPDECKEAVATLIYAAARFADLPELR 120
           GFLVERNVLRCY LIDEFCGTIS+QIPVL+KESECP+ECKEAVATLIYAAARFADLPELR
Sbjct: 61  GFLVERNVLRCYLLIDEFCGTISSQIPVLSKESECPEECKEAVATLIYAAARFADLPELR 120

Query: 121 ELRNLFTEKYGSSFGSFTNKEFIEKSRATTQTKEMKIQLLQEIAQETAIDWNSKALEQQL 180
           ELR+LFTEKYGSSFGSFTNKE IEKSRAT QTKEMKIQLLQEIAQ++AIDWNSKALEQQL
Sbjct: 121 ELRSLFTEKYGSSFGSFTNKELIEKSRATAQTKEMKIQLLQEIAQDSAIDWNSKALEQQL 180

Query: 181 YTPPPQNELDGERSGATKRNKTKVVSVPVYERKANSPRNKNNSDNESIFDSRSEGNTTET 240
           YTPPPQNE  GERS   KRNKTK+V+VPV+ERK  SPR K+NSDNESIFDSRSEGNTTET
Sbjct: 181 YTPPPQNEPAGERSATIKRNKTKIVTVPVHERKTISPRKKDNSDNESIFDSRSEGNTTET 240

Query: 241 STGDSTDQDIHKGVSEDEVEDQKPFNHRFVQPPYLKTKPIKTEANEKEPRKVTI-ENDES 300
           STGDSTDQD+HKG+SEDEVEDQKPF  RFV PPYLKTKPIKTEAN +E +KVT+  NDES
Sbjct: 241 STGDSTDQDVHKGISEDEVEDQKPFYLRFVPPPYLKTKPIKTEANVEESQKVTVGNNDES 300

Query: 301 N--NPKSPTEEKPKPRSVRRRNVKPQPARDINIDDVGSSTIDVTTKISSSRNKGKETMIG 360
           N  +PKSPTEEKPKPRSVRRRNVKPQPARDINIDD GSSTID   K S S+NKGKETMIG
Sbjct: 301 NKQHPKSPTEEKPKPRSVRRRNVKPQPARDINIDDAGSSTIDGAEKTSLSQNKGKETMIG 360

Query: 361 EEKGARDDEERVLDGLLMQYSKKKTNQESKSRGKSNLKPQRQQEKDNIEHQRPTT----- 420
           EE+GA DDEERVLDGLLMQYS+KKT+QES +RGK NLKPQRQQEKDN E Q+P +     
Sbjct: 361 EERGAGDDEERVLDGLLMQYSRKKTSQES-NRGKGNLKPQRQQEKDNAEPQKPKSTKTED 420

Query: 421 -----RAVSLPTDQNEPTKKHTRTNSFVHPKLPEYDQLAARIAALKEK 456
                RAVSLPTDQ+EP KKHTRTNSFVHPKLPEYDQLAARIAALKEK
Sbjct: 421 SFVPARAVSLPTDQSEPVKKHTRTNSFVHPKLPEYDQLAARIAALKEK 467

BLAST of Pay0001623 vs. NCBI nr
Match: KAG6575541.1 (IST1-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 612.5 bits (1578), Expect = 3.0e-171
Identity = 341/460 (74.13%), Postives = 375/460 (81.52%), Query Frame = 0

Query: 1   MFDMFLKPKFYTKCKSCVKMTKTRLDTIRKKKNAVLKYLKNDIVELLKSRLDYNAFNRAE 60
           MF MF KPKFYTKCKSCVKMTKTRLDTIRKKK  VLK+LKNDIVELLKSRLDYNA+NRAE
Sbjct: 1   MFRMFFKPKFYTKCKSCVKMTKTRLDTIRKKKKVVLKFLKNDIVELLKSRLDYNAYNRAE 60

Query: 61  GFLVERNVLRCYELIDEFCGTISNQIPVLNKESECPDECKEAVATLIYAAARFADLPELR 120
           GFLVE+NVLRCY+LIDEFCGTI   I VLNKESECPDECKEAVA+LIYAAARF+DLPELR
Sbjct: 61  GFLVEQNVLRCYDLIDEFCGTIFKHISVLNKESECPDECKEAVASLIYAAARFSDLPELR 120

Query: 121 ELRNLFTEKYGSSFGSFTNKEFIEKSRATTQTKEMKIQLLQEIAQETAIDWNSKALEQQL 180
            LR+LFT +YG+SFGSFTNKE +EK RA  QTKE K+QLLQEIAQE+AIDWNSKALEQQL
Sbjct: 121 VLRSLFTGRYGTSFGSFTNKELVEKLRAVAQTKETKLQLLQEIAQESAIDWNSKALEQQL 180

Query: 181 YTPPPQNELDGERSGATKRNKTKVVSVPVYERKANSPRNKNNSDNESIFDSRSEGNTTET 240
           Y  PPQNELD ERS +T RNK+K+VSVPVY R  NSPR K NSD++SIFDSRSEGNTTET
Sbjct: 181 YV-PPQNELDRERSTSTNRNKSKIVSVPVYGRTTNSPRKKENSDDDSIFDSRSEGNTTET 240

Query: 241 STGD-STDQDIHKG-VSEDEVEDQKPFNHRFVQPPYLKTKPIKTEANEKEPRKVTIENDE 300
           S GD STDQD+HKG VSEDEVEDQKPF  RF+ PPYLKTKP K E N +EP+ VT EN  
Sbjct: 241 SMGDSSTDQDVHKGVVSEDEVEDQKPFYLRFITPPYLKTKPTKKEENVEEPQNVTEEN-- 300

Query: 301 SNNPKSPTEEKPKPRSVRRRNVKPQPARDINIDDVGSSTIDVTTKISSSRNKGKETMIGE 360
            N+PK+  EEKPKPRSVRRR+ KPQPAR INIDD G S  D   KISS RNKGKE M GE
Sbjct: 301 RNDPKTAIEEKPKPRSVRRRHAKPQPARSINIDDAGGSAADGAGKISSGRNKGKEAMTGE 360

Query: 361 EKGARDDEERVLDGLLMQYSKKKTNQESKSRGKSNLKPQRQQEKDNIEHQRPTTRAVSLP 420
           EKG  DDEER+LDGLL  YSKKKT QE     K N +PQR +     +   P T+++SLP
Sbjct: 361 EKGVEDDEERMLDGLLKLYSKKKTRQED----KDNAEPQRPKNTRTEDAYVP-TKSLSLP 420

Query: 421 ---TDQNEPTKKHTRTNSFVHPKLPEYDQLAARIAALKEK 456
              T+  EP KKH R NS+VHPKLP+YDQLAAR AALKEK
Sbjct: 421 PPRTEPIEPVKKHARANSYVHPKLPDYDQLAARFAALKEK 452

BLAST of Pay0001623 vs. TAIR 10
Match: AT1G79910.1 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 256.5 bits (654), Expect = 3.9e-68
Identity = 156/334 (46.71%), Postives = 215/334 (64.37%), Query Frame = 0

Query: 1   MFDMFLKPKFYTKCKSCVKMTKTRLDTIRKKKNAVLKYLKNDIVELLKSRLDYNAFNRAE 60
           MFD   KPKFYTKCKS VK+TKTR+DT+++KKN+V KYLKNDIV+LLK+ LDYNA+ RAE
Sbjct: 1   MFDGLFKPKFYTKCKSLVKITKTRVDTVKRKKNSVCKYLKNDIVDLLKNSLDYNAYGRAE 60

Query: 61  GFLVERNVLRCYELIDEFCGTISNQIPVLNKESECPDECKEAVATLIYAAARFADLPELR 120
           G + E+  L CYE +++FC  +++ + +L K   CPDEC+EA+++L+YAAAR +++PELR
Sbjct: 61  GLIEEKRRLACYEFLEQFCNCVASNVSLLQKSIRCPDECREAISSLVYAAARVSEVPELR 120

Query: 121 ELRNLFTEKYGSSFGSFTNKEFIEKSRATTQTKEMKIQLLQEIAQETAIDWNSKALEQQL 180
           +LR+LF E+YG++   F N EF+E+ +A   +KEMK++LLQEIA+E +I W++K+LEQ+L
Sbjct: 121 DLRSLFAERYGNTLDQFVNPEFVERFKAEPPSKEMKVELLQEIAREYSIKWDAKSLEQRL 180

Query: 181 YTPPPQNELDGERSGATKRNKTKVVSVPVYERKANSPRNKNNSDNESIF-----DSRSEG 240
           YTPP  + +  E    T+  KT+         K NS   K      S+      DS  + 
Sbjct: 181 YTPPHHHHVHTE----TEEPKTE---------KTNSEPEKTMEKRSSLSFHGREDSMDDS 240

Query: 241 NTTETSTGD----STDQDIHKGVSEDEVEDQKPFNHRFVQP-PYLKTKPIKTEANEKEPR 300
           N +  ST +    ST      G  +D   + KPF +RF+ P PY  TKP K E  E  P 
Sbjct: 241 NKSIMSTSEEDSMSTSGSCMTGPEDD--PEMKPFYYRFMTPAPY--TKP-KIEKQESLPE 300

Query: 301 KVTIENDESNNPKSPTEEKPKPRSVRRRNVKPQP 325
           K+T       + +SPT  KPKPRSVRRR   P P
Sbjct: 301 KMT--TSHLMDTESPTGGKPKPRSVRRRFANPPP 314

BLAST of Pay0001623 vs. TAIR 10
Match: AT1G79910.2 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 170.2 bits (430), Expect = 3.7e-42
Identity = 113/275 (41.09%), Postives = 163/275 (59.27%), Query Frame = 0

Query: 60  EGFLVERNVLRCYELIDEFCGTISNQIPVLNKESECPDECKEAVATLIYAAARFADLPEL 119
           EG + E+  L CYE +++FC  +++ + +L K   CPDEC+EA+++L+YAAAR +++PEL
Sbjct: 46  EGLIEEKRRLACYEFLEQFCNCVASNVSLLQKSIRCPDECREAISSLVYAAARVSEVPEL 105

Query: 120 RELRNLFTEKYGSSFGSFTNKEFIEKSRATTQTKEMKIQLLQEIAQETAIDWNSKALEQQ 179
           R+LR+LF E+YG++   F N EF+E+ +A   +KEMK++LLQEIA+E +I W++K+LEQ+
Sbjct: 106 RDLRSLFAERYGNTLDQFVNPEFVERFKAEPPSKEMKVELLQEIAREYSIKWDAKSLEQR 165

Query: 180 LYTPPPQNELDGERSGATKRNKTKVVSVPVYERKANSPRNKNNSDNESIF-----DSRSE 239
           LYTPP  + +  E    T+  KT+         K NS   K      S+      DS  +
Sbjct: 166 LYTPPHHHHVHTE----TEEPKTE---------KTNSEPEKTMEKRSSLSFHGREDSMDD 225

Query: 240 GNTTETSTGD----STDQDIHKGVSEDEVEDQKPFNHRFVQP-PYLKTKPIKTEANEKEP 299
            N +  ST +    ST      G  +D   + KPF +RF+ P PY  TKP K E  E  P
Sbjct: 226 SNKSIMSTSEEDSMSTSGSCMTGPEDD--PEMKPFYYRFMTPAPY--TKP-KIEKQESLP 285

Query: 300 RKVTIENDESNNPKSPTEEKPKPRSVRRRNVKPQP 325
            K+T       + +SPT  KPKPRSVRRR   P P
Sbjct: 286 EKMT--TSHLMDTESPTGGKPKPRSVRRRFANPPP 300

BLAST of Pay0001623 vs. TAIR 10
Match: AT4G32350.1 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 147.9 bits (372), Expect = 2.0e-35
Identity = 96/313 (30.67%), Postives = 161/313 (51.44%), Query Frame = 0

Query: 1   MFDMFLKPKFYTKCKSCVKMTKTRLDTIRKKKNAVLKYLKNDIVELLKSRLDYNAFNRAE 60
           MFD FL   F  K K  +K+TK R+D +R+K+NA +K+LK D+ +L+ +  DYNAF+RA 
Sbjct: 1   MFDGFLGRGFAPKGKPLIKLTKNRIDVLRRKRNATIKFLKRDLADLIINGHDYNAFSRAG 60

Query: 61  GFLVERNVLRCYELIDEFCGTISNQIPVLNKESECPDECKEAVATLIYAAARFADLPELR 120
           G L E   L   + +++ C  +  Q+  + K  ECP++C+EA+++L++AA+ F++LPELR
Sbjct: 61  GLLDELRYLWSLDFVEQTCDFVYKQLSTMQKTPECPEDCREAISSLMFAASGFSELPELR 120

Query: 121 ELRNLFTEKYGSSFGSFTNKEFIEKSRATTQTKEMKIQLLQEIAQETAIDWNSKALEQQL 180
           ELR +F EKY  S   F N+E +E   +   + E K++L++++A E +I W+SK  E+++
Sbjct: 121 ELRQMFHEKYTDSLALFVNQELVENMSSKPFSMEKKVKLMEDVALEFSIRWDSKDFEKRI 180

Query: 181 ------------------YTP-------PPQNELDGERSGATKRNKTKVVSV---PVYER 240
                             Y P       P + E +G  +G +   KT   S    P+++ 
Sbjct: 181 VRQNSISVMETPKSTNDKYKPVDRNMALPKREEFEGSENGVSLNRKTAEASERRDPLFQS 240

Query: 241 KANSPRNKNNSDNESIFDSRSEGNTTETSTGDSTDQDIHKGVSEDEVEDQKPFNHRFVQP 286
              S +N    +   +       N    S  +S D         ++ E ++ + H    P
Sbjct: 241 DKESYQNGLRGNQRGLTYKERSENVLHASRSESKD---------NKAERKEFYLHSKQNP 300

BLAST of Pay0001623 vs. TAIR 10
Match: AT1G52315.1 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 136.0 bits (341), Expect = 7.8e-32
Identity = 108/345 (31.30%), Postives = 177/345 (51.30%), Query Frame = 0

Query: 1   MFDMFLKPKFYTKCKSCVKMTKTRLDTIRKKKNAVLKYLKNDIVELLKSRLDYNAFNRAE 60
           MF    KPKFY K KS     K R+D +R+K+ A+++  K DIV  LK+  D  A+ RAE
Sbjct: 1   MFLRCFKPKFYKKSKSTTSYMKIRIDIVRRKRIAMVRNYKTDIVNFLKNGQDSEAYRRAE 60

Query: 61  GFLVERNVLRCYELIDEFCGTISNQIPVLNKESECPDECKEAVATLIYAAARFADLPELR 120
             L E  ++ CY+LI+ FC  IS  + ++ K+ ECP+EC+EAV++LIYA A   D+PEL+
Sbjct: 61  LLLEELRIISCYDLIERFCDCISENLSLMLKKRECPEECREAVSSLIYATAWVPDVPELK 120

Query: 121 ELRNLFTEKYGSSFGSFTNKEFIEKSR-ATTQTKEMKIQLLQEIAQETAIDWNSKALEQQ 180
           +LR +FT+++G+   S  N E +EK+      ++E+KIQ ++++A E +I+W+   L+  
Sbjct: 121 DLRAVFTKRFGNFIASSVNHELVEKTELLRPPSRELKIQTVKDVANEFSINWDPTPLKLM 180

Query: 181 LYTPPPQNELDGERSGATKRNKTKVVSVPVYERK-ANSPRNKNNSDNESI------FDSR 240
           L            R  +  + K KV +   Y  K         +SD+ES+       DS 
Sbjct: 181 LL-----------RETSALQIKDKVETRADYLNKDTEKSFMDEHSDDESVLSESWRIDSL 240

Query: 241 SEGNTTETSTGDSTDQDIHKGVSEDEVEDQKPFNHRFVQPPYLKT---KPIKTEANEKEP 300
           S+G+ + +S+  S+     + VS  + + +K   +  +  P  K       K +  ++E 
Sbjct: 241 SKGSLSSSSSSSSSSL---RRVSVRKKKGKKILPYGIISSPDTKLGAGNDEKAQEEKEEK 300

Query: 301 RKVTIENDE--------------SNNPKSPTEEKPKPRSVRRRNV 321
           RK+  + +               SNN  S   E  +  S +R N+
Sbjct: 301 RKIADQENSRLLGIQGSRSLTSTSNNEVSTIRENERMSSFQRPNL 331

BLAST of Pay0001623 vs. TAIR 10
Match: AT4G35730.1 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 101.7 bits (252), Expect = 1.6e-21
Identity = 117/462 (25.32%), Postives = 208/462 (45.02%), Query Frame = 0

Query: 12  TKCKSCVKMTKTRLDTIRKKKNAVLKYLKNDIVELLKSRLDYNAFNRAEGFLVERNVLRC 71
           +KCK+  KM   R+  IR K+  V+K ++ DI  LL+S  D  A  R E  + E+N+   
Sbjct: 29  SKCKTAAKMAVARIKLIRNKRLVVVKQMRRDIAVLLQSGQDATARIRVEHVIREQNIQAA 88

Query: 72  YELIDEFCGTISNQIPVLNKESECPDECKEAVATLIYAAARFADLPELRELRNLFTEKYG 131
            E+I+ FC  I +++ ++ K+ +CP + KE +A+LI+AA R +++PEL +LR++F +KYG
Sbjct: 89  NEIIELFCELIVSRLTIITKQKQCPVDLKEGIASLIFAAPRCSEIPELGDLRDIFAKKYG 148

Query: 132 SSFGSF---------TNKEFIEKSRATTQTKEMKIQLLQEIAQETAIDWNSKALEQQLYT 191
             F S           N+  I+K        E K+++++EIA+E  +DW++   EQ+L  
Sbjct: 149 KDFVSAATDLRPSCGVNRMLIDKLSVRNPGGEYKLKIMKEIAKEFQVDWDTTETEQEL-L 208

Query: 192 PPPQNELDGER---SGATKRNKTKVVSVPVYERKANSPRNKNNSDNESIFDSRSEGN-TT 251
            P +  +DG R   S ++       ++ P+   KA      + S N    D+ S     T
Sbjct: 209 KPQEESIDGPRKFVSASSLPVNRAAINEPIDPTKAVPRSTSSMSINTHYHDTESAAEAAT 268

Query: 252 ETSTGDSTDQDIHKGVSEDEVEDQKPFNHRFVQPPYLK-TKPIKTEANEKEPRKVTIEND 311
           E +        +   ++       K F+       + K ++ +    +    R+ + +++
Sbjct: 269 ELAKQAVAAAQVASLLATRRDSSNKEFSVSSDHSTHQKDSQYMDHHHHHPGSRRQSRDSE 328

Query: 312 ESNNPKSPTEEKPKPRSVRRRNVKPQPARDINIDDVGSSTIDVTTKISSSRNKGKETMIG 371
            S+    P  E    R + RR+    P         G +  D   + +++  + KETM  
Sbjct: 329 TSSYYAKPGAEN---RGMGRRHSYNNP---------GINESDYEEEYTNTEAEAKETMRR 388

Query: 372 EEKGARDDEERVLDGLLMQYSKKKTNQESKSRGKSNLKPQRQQEKDNIEHQRPTTRAVSL 431
                              Y+ +     + S  K +     ++E +  E  +   R  SL
Sbjct: 389 RH----------------SYNPRSVPPPATSEIKFDESDYYEEETEPDEPSQ--GRVSSL 448

Query: 432 PTDQNEP------TKKHTRTNSFVHPKLPEYDQLAARIAALK 454
           P ++  P        +   +   VHPKLP+YD LAAR  A++
Sbjct: 449 PPNRAPPQAPQSGESRQDSSGHQVHPKLPDYDILAARFEAIR 459

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q54I398.4e-1526.92IST1-like protein OS=Dictyostelium discoideum OX=44689 GN=DDB_G0289029 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
A0A1S3CGT55.7e-24899.78uncharacterized protein LOC103500577 OS=Cucumis melo OX=3656 GN=LOC103500577 PE=... [more]
A0A5D3BX006.5e-23699.77Putative Regulator of Vps4 activity in the MVB pathway protein OS=Cucumis melo v... [more]
A0A0A0K9263.0e-23395.18Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G446760 PE=3 SV=1[more]
A0A6J1GMM19.5e-17173.91uncharacterized protein LOC111455825 OS=Cucurbita moschata OX=3662 GN=LOC1114558... [more]
A0A6J1JV698.1e-17073.26uncharacterized protein LOC111488629 OS=Cucurbita maxima OX=3661 GN=LOC111488629... [more]
Match NameE-valueIdentityDescription
XP_008462153.11.2e-24799.78PREDICTED: uncharacterized protein LOC103500577 [Cucumis melo][more]
KAA0059291.11.3e-23599.77putative Regulator of Vps4 activity in the MVB pathway protein [Cucumis melo var... [more]
XP_004141848.16.2e-23395.18uncharacterized protein LOC101220202 [Cucumis sativus] >KGN45359.1 hypothetical ... [more]
XP_038897244.11.4e-20885.04uncharacterized protein LOC120085370 [Benincasa hispida][more]
KAG6575541.13.0e-17174.13IST1-like protein, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
AT1G79910.13.9e-6846.71Regulator of Vps4 activity in the MVB pathway protein [more]
AT1G79910.23.7e-4241.09Regulator of Vps4 activity in the MVB pathway protein [more]
AT4G32350.12.0e-3530.67Regulator of Vps4 activity in the MVB pathway protein [more]
AT1G52315.17.8e-3231.30Regulator of Vps4 activity in the MVB pathway protein [more]
AT4G35730.11.6e-2125.32Regulator of Vps4 activity in the MVB pathway protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (Payzawat) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR042277Vacuolar protein sorting-associated protein IST1-likeGENE3D1.20.1260.60coord: 6..177
e-value: 2.1E-49
score: 169.6
IPR005061Vacuolar protein sorting-associated protein Ist1PFAMPF03398Ist1coord: 15..169
e-value: 2.1E-43
score: 148.1
IPR005061Vacuolar protein sorting-associated protein Ist1PANTHERPTHR12161IST1 FAMILY MEMBERcoord: 9..455
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 220..244
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 245..265
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 334..351
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 181..439
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 352..374
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 382..409
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 280..325
NoneNo IPR availablePANTHERPTHR12161:SF60REGULATOR OF VPS4 ACTIVITY IN THE MVB PATHWAY PROTEINcoord: 9..455

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Pay0001623.1Pay0001623.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0015031 protein transport