PI0027335 (gene) Melon (PI 482460) v1

Overview
NamePI0027335
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
DescriptionEIN3-binding F-box protein 1
Locationchr04: 30118306 .. 30120860 (-)
RNA-Seq ExpressionPI0027335
SyntenyPI0027335
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCGAAGCTCTTCGCCTTTTCCGGTAAGTTTATGGGTTTTTACTTTTTTTTATTTTTTTATTTATTTATTTTTGTTTCGTTTTGCCTACTTTGAAATTTGACTAGATTGGGTGGCTGGTTGGTTTAAGCGTCGTGTTTTGTATGGGTGTTGGATTATTATTTGGATGAAAGTTTGGATCGTAGGGAAAGATTTGGAATTATTGGTTTAGTTTTTGAGGTTTCTTGATTCGCCAGGTGGCGGATCATGTCTTGGTATGAATTGTGAGGGAATATGGATTTGGGTTTCTTCTCTTGGGATTTCGTTTTATGGTGTTGATTGATTGGCCGTTTTATTGTCTTGAACAGTCCATGCCAGATGTAAGTTTCTTGAAAAGAGATATCATAGTTTGAAGATAGGTTTACCTGTTAAGTGATATGTATGTGTTATTGATCTATCATTCCCATACAGATTTAGATTTGAAGTTTTGAATAAGATAGCACATCAATAGTAAATATTTAAGGGTCAAATATAGTTTTGCAGAGATTACTTCTTGTTTTTCTCTGTTGATAAATTTTCGATCATTTGATCTAGAAGTTGAGGGGTATTTTGATCTGAGGATTTATTTGTGAAGTCGAATTATGTATCTAACTTGTAGTTCTTGTTGTTGAAATTTCAGGTAGTGAAGATTTTTGCCCTGGAGGGTCAATATACCCAAATCCCAAGGACTCAAGTTTATTCTTGTCCCTTCCTCACCACGTCGATGTCTATTTTCCTCCTCGAAAGAGGTCTCGCATCACTGCTCCATTTGTGTTTGGTGGAGAAGAAGTTGAATCAAAAGCAAATGTTTCTATCGAGATTCTTCCGGATGAGTGCCTGTTTGAGATTTTCAGACGATTGTCTGGTGGCAAAGAAAGGAGTGCCTGCGCAACCGTTTCTAAACGATGGCTTATGCTTTTGAGCAATATATATTCCCACGAGTTGAAATCTGAGGATGAGGTGGCCTCAAAGGAGGTTGAAGACATTGAAATTGAGAGTGATGGATATCTGTCTAGGAGTTTGGAAGGCAAGCAGGCGACAGATCTCAGACTTGCTGCCATTTCAGTTGGAACTGCAAGTCGAGGGGGTTTAGGTAAGCTTGTGATTCGGGGAAACAATCATGTCAGTAAGGTTACAGACCTCGGACTTAAGGCAATTGCTCGTGGGTGTCAATCCCTGAGGGCTCTTTCTTTGTGGAATCTTTCCTCTATTAGAGATGAAGGTCTTTGTGAGATTGCCAAGGCGTCTCACCAGTTGGAGAAGCTTGATCTCTGTCGTTGCCCTGCTGTATCTGACAAGGCTTTGGTTGAAATAGCAAGGAACTGCCCAAAGTTGACTGATATTACAATTGAATCTTGTGCGAAAATTGGAAATGAAAGTCTGCGGGCTATTGGGCAGTTCTGCCCCAAGCTGAAGTCTATTGTGATTAAAGATTGCCCACTCGTTGGAGATCAGGGAATTGCTAGTTTGTTGTCTTTGAACACTTGTGCCTTGAATAAGGTGAAGCTTCAGGCGTTGAACGTTTCCGATGTGTCCCTTGCTGTTATTGGGCACTATGGCAAGGCAGTTACTGACCTTGTCCTTACCGATCTTAAGAATGTTAGTGAAAAAGGGTTCTGGGTCATGGGTAATGGTCATGGACTACAAAAATTGAAGTCATTCACAATTTCCTCCTGCAATGGAGTGACAGACATGGGCCTTGAATCTGTTGGAAAGGGTAGTCCAAATTTAAAGCATTTCTGTCTAAGGAAGTGTTCGTTTTATCTGATAATGGCCTGGTCTCTTTTGCAAAAGCTGCAAGGTCGCTAGAATGCTTGCAATTGGAAGAGTGCCACAGGATCACTCAGTTTGGATTCTTTGGTGTCGTTTTGAACTGCAGTGCAACTTTGAAGGCTCTTTCTCTCATAAGCTGCTTGGGAATTAAGGACATTAACTCAGAATTTCCTATCCCAGCCTCTAGCGGATCCTTACGGTCGTTGACCATCCGCAACTGCCATGGTTTTGGCAACAGAAACCTTGCTTTACTGGGCAAGCTATGCCCCCAGTTGCAAAATGTGGATTTTAGTGGACTTGTTGGAATTGATGATTGTGGATTTCTAGCTTGGCTCCAAAACTGTCAGTCGGGTTTAGTGAAGATCAATGTTAATGGTTGTGTCAACCTGACCGACAAGGTTGTTTCATCCATAATCGAGCACCATGGTTGGTCCCTTAAAATGCTTAATCTCGATGGTTGTAAGAAGATCACTGATGCTAGCATGACTTCCATTGCAAACAACTGCCCATTACTCAGTGATCTTGATGTCTCAAAATGCTCGATCACAGATTCAGGAATTGCAGCTCTAGCTCATGCCAAGCGACTGAATCTGCAGATCTTTTCGATTTCTGGTTGCTCATTCGTGTCAGACAAAAGCTTGGCAGACTTGTTAAACTTGGGTGAGACTCTTGTGGGTCTCAACATCCAGCATTGTAATGCTATCAGCAGTAGCACTGTTGATCTGCTTGTGGAGCAGCTCTGGAGATGTGACATTCTTTCATAA

mRNA sequence

ATGTCGAAGCTCTTCGCCTTTTCCGGTAGTGAAGATTTTTGCCCTGGAGGGTCAATATACCCAAATCCCAAGGACTCAAGTTTATTCTTGTCCCTTCCTCACCACGTCGATGTCTATTTTCCTCCTCGAAAGAGGTCTCGCATCACTGCTCCATTTGTGTTTGGTGGAGAAGAAGTTGAATCAAAAGCAAATGTTTCTATCGAGATTCTTCCGGATGAGTGCCTGTTTGAGATTTTCAGACGATTGTCTGGTGGCAAAGAAAGGAGTGCCTGCGCAACCGTTTCTAAACGATGGCTTATGCTTTTGAGCAATATATATTCCCACGAGTTGAAATCTGAGGATGAGGTGGCCTCAAAGGAGGTTGAAGACATTGAAATTGAGAGTGATGGATATCTGTCTAGGAGTTTGGAAGGCAAGCAGGCGACAGATCTCAGACTTGCTGCCATTTCAGTTGGAACTGCAAGTCGAGGGGGTTTAGGTAAGCTTGTGATTCGGGGAAACAATCATGTCAGTAAGGTTACAGACCTCGGACTTAAGGCAATTGCTCGTGGGTGTCAATCCCTGAGGGCTCTTTCTTTGTGGAATCTTTCCTCTATTAGAGATGAAGGTCTTTGTGAGATTGCCAAGGCGTCTCACCAGTTGGAGAAGCTTGATCTCTGTCGTTGCCCTGCTGTATCTGACAAGGCTTTGGTTGAAATAGCAAGGAACTGCCCAAAGTTGACTGATATTACAATTGAATCTTGTGCGAAAATTGGAAATGAAAGTCTGCGGGCTATTGGGCAGTTCTGCCCCAAGCTGAAGTCTATTGTGATTAAAGATTGCCCACTCGTTGGAGATCAGGGAATTGCTAGTTTGTTGTCTTTGAACACTTGTGCCTTGAATAAGGTGAAGCTTCAGGCGTTGAACGTTTCCGATGTGTCCCTTGCTGTTATTGGGCACTATGGCAAGGCAGTTACTGACCTTGTCCTTACCGATCTTAAGAATGTTAGTGAAAAAGGGTTCTGGGTCATGGGTAATGGTCATGGACTACAAAAATTGAAGTCATTCACAATTTCCTCCTGCAATGGAGTGACAGACATGGGCCTTGAATCTGTTGGAAAGGGTAGTCCAAATTTAAAGCATTTCTGTCTAAGGAAGTCTGCAAGGTCGCTAGAATGCTTGCAATTGGAAGAGTGCCACAGGATCACTCAGTTTGGATTCTTTGGTGTCGTTTTGAACTGCAGTGCAACTTTGAAGGCTCTTTCTCTCATAAGCTGCTTGGGAATTAAGGACATTAACTCAGAATTTCCTATCCCAGCCTCTAGCGGATCCTTACGGTCGTTGACCATCCGCAACTGCCATGGTTTTGGCAACAGAAACCTTGCTTTACTGGGCAAGCTATGCCCCCAGTTGCAAAATGTGGATTTTAGTGGACTTGTTGGAATTGATGATTGTGGATTTCTAGCTTGGCTCCAAAACTGTCAGTCGGGTTTAGTGAAGATCAATGTTAATGGTTGTGTCAACCTGACCGACAAGGTTGTTTCATCCATAATCGAGCACCATGGTTGGTCCCTTAAAATGCTTAATCTCGATGGTTGTAAGAAGATCACTGATGCTAGCATGACTTCCATTGCAAACAACTGCCCATTACTCAGTGATCTTGATGTCTCAAAATGCTCGATCACAGATTCAGGAATTGCAGCTCTAGCTCATGCCAAGCGACTGAATCTGCAGATCTTTTCGATTTCTGGTTGCTCATTCGTGTCAGACAAAAGCTTGGCAGACTTGTTAAACTTGGGTGAGACTCTTGTGGGTCTCAACATCCAGCATTGTAATGCTATCAGCAGTAGCACTGTTGATCTGCTTGTGGAGCAGCTCTGGAGATGTGACATTCTTTCATAA

Coding sequence (CDS)

ATGTCGAAGCTCTTCGCCTTTTCCGGTAGTGAAGATTTTTGCCCTGGAGGGTCAATATACCCAAATCCCAAGGACTCAAGTTTATTCTTGTCCCTTCCTCACCACGTCGATGTCTATTTTCCTCCTCGAAAGAGGTCTCGCATCACTGCTCCATTTGTGTTTGGTGGAGAAGAAGTTGAATCAAAAGCAAATGTTTCTATCGAGATTCTTCCGGATGAGTGCCTGTTTGAGATTTTCAGACGATTGTCTGGTGGCAAAGAAAGGAGTGCCTGCGCAACCGTTTCTAAACGATGGCTTATGCTTTTGAGCAATATATATTCCCACGAGTTGAAATCTGAGGATGAGGTGGCCTCAAAGGAGGTTGAAGACATTGAAATTGAGAGTGATGGATATCTGTCTAGGAGTTTGGAAGGCAAGCAGGCGACAGATCTCAGACTTGCTGCCATTTCAGTTGGAACTGCAAGTCGAGGGGGTTTAGGTAAGCTTGTGATTCGGGGAAACAATCATGTCAGTAAGGTTACAGACCTCGGACTTAAGGCAATTGCTCGTGGGTGTCAATCCCTGAGGGCTCTTTCTTTGTGGAATCTTTCCTCTATTAGAGATGAAGGTCTTTGTGAGATTGCCAAGGCGTCTCACCAGTTGGAGAAGCTTGATCTCTGTCGTTGCCCTGCTGTATCTGACAAGGCTTTGGTTGAAATAGCAAGGAACTGCCCAAAGTTGACTGATATTACAATTGAATCTTGTGCGAAAATTGGAAATGAAAGTCTGCGGGCTATTGGGCAGTTCTGCCCCAAGCTGAAGTCTATTGTGATTAAAGATTGCCCACTCGTTGGAGATCAGGGAATTGCTAGTTTGTTGTCTTTGAACACTTGTGCCTTGAATAAGGTGAAGCTTCAGGCGTTGAACGTTTCCGATGTGTCCCTTGCTGTTATTGGGCACTATGGCAAGGCAGTTACTGACCTTGTCCTTACCGATCTTAAGAATGTTAGTGAAAAAGGGTTCTGGGTCATGGGTAATGGTCATGGACTACAAAAATTGAAGTCATTCACAATTTCCTCCTGCAATGGAGTGACAGACATGGGCCTTGAATCTGTTGGAAAGGGTAGTCCAAATTTAAAGCATTTCTGTCTAAGGAAGTCTGCAAGGTCGCTAGAATGCTTGCAATTGGAAGAGTGCCACAGGATCACTCAGTTTGGATTCTTTGGTGTCGTTTTGAACTGCAGTGCAACTTTGAAGGCTCTTTCTCTCATAAGCTGCTTGGGAATTAAGGACATTAACTCAGAATTTCCTATCCCAGCCTCTAGCGGATCCTTACGGTCGTTGACCATCCGCAACTGCCATGGTTTTGGCAACAGAAACCTTGCTTTACTGGGCAAGCTATGCCCCCAGTTGCAAAATGTGGATTTTAGTGGACTTGTTGGAATTGATGATTGTGGATTTCTAGCTTGGCTCCAAAACTGTCAGTCGGGTTTAGTGAAGATCAATGTTAATGGTTGTGTCAACCTGACCGACAAGGTTGTTTCATCCATAATCGAGCACCATGGTTGGTCCCTTAAAATGCTTAATCTCGATGGTTGTAAGAAGATCACTGATGCTAGCATGACTTCCATTGCAAACAACTGCCCATTACTCAGTGATCTTGATGTCTCAAAATGCTCGATCACAGATTCAGGAATTGCAGCTCTAGCTCATGCCAAGCGACTGAATCTGCAGATCTTTTCGATTTCTGGTTGCTCATTCGTGTCAGACAAAAGCTTGGCAGACTTGTTAAACTTGGGTGAGACTCTTGTGGGTCTCAACATCCAGCATTGTAATGCTATCAGCAGTAGCACTGTTGATCTGCTTGTGGAGCAGCTCTGGAGATGTGACATTCTTTCATAA

Protein sequence

MSKLFAFSGSEDFCPGGSIYPNPKDSSLFLSLPHHVDVYFPPRKRSRITAPFVFGGEEVESKANVSIEILPDECLFEIFRRLSGGKERSACATVSKRWLMLLSNIYSHELKSEDEVASKEVEDIEIESDGYLSRSLEGKQATDLRLAAISVGTASRGGLGKLVIRGNNHVSKVTDLGLKAIARGCQSLRALSLWNLSSIRDEGLCEIAKASHQLEKLDLCRCPAVSDKALVEIARNCPKLTDITIESCAKIGNESLRAIGQFCPKLKSIVIKDCPLVGDQGIASLLSLNTCALNKVKLQALNVSDVSLAVIGHYGKAVTDLVLTDLKNVSEKGFWVMGNGHGLQKLKSFTISSCNGVTDMGLESVGKGSPNLKHFCLRKSARSLECLQLEECHRITQFGFFGVVLNCSATLKALSLISCLGIKDINSEFPIPASSGSLRSLTIRNCHGFGNRNLALLGKLCPQLQNVDFSGLVGIDDCGFLAWLQNCQSGLVKINVNGCVNLTDKVVSSIIEHHGWSLKMLNLDGCKKITDASMTSIANNCPLLSDLDVSKCSITDSGIAALAHAKRLNLQIFSISGCSFVSDKSLADLLNLGETLVGLNIQHCNAISSSTVDLLVEQLWRCDILS
Homology
BLAST of PI0027335 vs. ExPASy Swiss-Prot
Match: Q9SKK0 (EIN3-binding F-box protein 1 OS=Arabidopsis thaliana OX=3702 GN=EBF1 PE=1 SV=1)

HSP 1 Score: 757.7 bits (1955), Expect = 1.1e-217
Identity = 387/640 (60.47%), Postives = 491/640 (76.72%), Query Frame = 0

Query: 1   MSKLFAFSGSEDFCPGGSIYPNPKDSSLFLSLPHHVDVYFPPRKRSRITAPFVFGGEEVE 60
           MS++F+F+G  DF   G+IYPNPKD+SL LSL    DVYFPP KRSR+ AP +F   E  
Sbjct: 1   MSQIFSFAGENDFYRRGAIYPNPKDASLLLSLGSFADVYFPPSKRSRVVAPTIFSAFE-- 60

Query: 61  SKANVSIEILPDECLFEIFRRLSGGKERSACATVSKRWLMLLSNIYSHELKSEDEVASKE 120
            K  VSI++LPDECLFEIFRRLSG +ERSACA VSK+WL L+S+I   E+    +V SK 
Sbjct: 61  -KKPVSIDVLPDECLFEIFRRLSGPQERSACAFVSKQWLTLVSSIRQKEI----DVPSKI 120

Query: 121 VEDIEIESDGYLSRSLEGKQATDLRLAAISVGTASRGGLGKLVIRGNNHVSKVTDLGLKA 180
            ED + + +G LSRSL+GK+ATD+RLAAI+VGTA RGGLGKL IRG+N  +KV+DLGL++
Sbjct: 121 TEDGD-DCEGCLSRSLDGKKATDVRLAAIAVGTAGRGGLGKLSIRGSNS-AKVSDLGLRS 180

Query: 181 IARGCQSLRALSLWNLSSIRDEGLCEIAKASHQLEKLDLCRCPAVSDKALVEIARNCPKL 240
           I R C SL +LSLWN+S+I D GL EIA+   QLEKL+L RC  ++DK LV IA++CP L
Sbjct: 181 IGRSCPSLGSLSLWNVSTITDNGLLEIAEGCAQLEKLELNRCSTITDKGLVAIAKSCPNL 240

Query: 241 TDITIESCAKIGNESLRAIGQFCPKLKSIVIKDCPLVGDQGIASLLSLNTCALNKVKLQA 300
           T++T+E+C++IG+E L AI + C KLKS+ IK+CPLV DQGIASLLS  TC+L K+KLQ 
Sbjct: 241 TELTLEACSRIGDEGLLAIARSCSKLKSVSIKNCPLVRDQGIASLLSNTTCSLAKLKLQM 300

Query: 301 LNVSDVSLAVIGHYGKAVTDLVLTDLKNVSEKGFWVMGNGHGLQKLKSFTISSCNGVTDM 360
           LNV+DVSLAV+GHYG ++TDLVL  L +VSEKGFWVMGNG GLQKL S TI++C GVTDM
Sbjct: 301 LNVTDVSLAVVGHYGLSITDLVLAGLSHVSEKGFWVMGNGVGLQKLNSLTITACQGVTDM 360

Query: 361 GLESVGKGSPNLKHFCLRKS--------------ARSLECLQLEECHRITQFGFFGVVLN 420
           GLESVGKG PN+K   + KS              + SLE LQLEECHR+TQFGFFG +LN
Sbjct: 361 GLESVGKGCPNMKKAIISKSPLLSDNGLVSFAKASLSLESLQLEECHRVTQFGFFGSLLN 420

Query: 421 CSATLKALSLISCLGIKDINSEFPIPASSGSLRSLTIRNCHGFGNRNLALLGKLCPQLQN 480
           C   LKA SL++CL I+D+ +  P  +   +LRSL+IRNC GFG+ NLA +GKLCPQL++
Sbjct: 421 CGEKLKAFSLVNCLSIRDLTTGLPASSHCSALRSLSIRNCPGFGDANLAAIGKLCPQLED 480

Query: 481 VDFSGLVGIDDCGFLAWLQNCQSGLVKINVNGCVNLTDKVVSSIIEHHGWSLKMLNLDGC 540
           +D  GL GI + GFL  +   QS LVKIN +GC NLTD+V+S+I   +GW+L++LN+DGC
Sbjct: 481 IDLCGLKGITESGFLHLI---QSSLVKINFSGCSNLTDRVISAITARNGWTLEVLNIDGC 540

Query: 541 KKITDASMTSIANNCPLLSDLDVSKCSITDSGIAALAHAKRLNLQIFSISGCSFVSDKSL 600
             ITDAS+ SIA NC +LSDLD+SKC+I+DSGI ALA + +L LQI S++GCS V+DKSL
Sbjct: 541 SNITDASLVSIAANCQILSDLDISKCAISDSGIQALASSDKLKLQILSVAGCSMVTDKSL 600

Query: 601 ADLLNLGETLVGLNIQHCNAISSSTVDLLVEQLWRCDILS 627
             ++ LG TL+GLN+Q C +IS+STVD LVE+L++CDILS
Sbjct: 601 PAIVGLGSTLLGLNLQQCRSISNSTVDFLVERLYKCDILS 628

BLAST of PI0027335 vs. ExPASy Swiss-Prot
Match: Q708Y0 (EIN3-binding F-box protein 2 OS=Arabidopsis thaliana OX=3702 GN=EBF2 PE=1 SV=1)

HSP 1 Score: 669.5 bits (1726), Expect = 3.8e-191
Identity = 359/641 (56.01%), Postives = 449/641 (70.05%), Query Frame = 0

Query: 1   MSKLFAFSGSEDFCPGGSIYPNPKDSSLFLSLPHHVDVYFPPRKRSRITAPFVFGGEEVE 60
           MS +F FSG ED   GGS+Y +P       S P    VY+P RKR R+ A   + G E  
Sbjct: 1   MSGIFRFSGDEDCLLGGSMYLSPG------SCP---GVYYPARKRLRVAATSFYSGFE-- 60

Query: 61  SKANVSIEILPDECLFEIFRRLSGGKERSACATVSKRWLMLLSNIYSHELKSEDEVASKE 120
            +   SI++LP+ECLFEI RRL  G+ERSACA VSK WL LLS+I      S  EV    
Sbjct: 61  -EKQTSIDVLPEECLFEILRRLPSGQERSACACVSKHWLNLLSSI------SRSEVNESS 120

Query: 121 VEDIEIESDGYLSRSLEGKQATDLRLAAISVGTASRGGLGKLVIRGNNHVSKVTDLGLKA 180
           V+D+E E +G+LSRSLEGK+ATDLRLAAI+VGT+SRGGLGKL IRG+   SKVTD+GL A
Sbjct: 121 VQDVE-EGEGFLSRSLEGKKATDLRLAAIAVGTSSRGGLGKLQIRGSGFESKVTDVGLGA 180

Query: 181 IARGCQSLRALSLWNLSSIRDEGLCEIAKASHQLEKLDLCRCPAVSDKALVEIARNCPKL 240
           +A GC SLR +SLWNL ++ D GL EIA++   +EKLDL RCP ++D  LV IA NC  L
Sbjct: 181 VAHGCPSLRIVSLWNLPAVSDLGLSEIARSCPMIEKLDLSRCPGITDSGLVAIAENCVNL 240

Query: 241 TDITIESCAKIGNESLRAIGQFCPKLKSIVIKDCPLVGDQGIASLLSLNTCALNKVKLQA 300
           +D+TI+SC+ +GNE LRAI + C  L+SI I+ CP +GDQG+A LL+     L KVKLQ 
Sbjct: 241 SDLTIDSCSGVGNEGLRAIARRCVNLRSISIRSCPRIGDQGVAFLLAQAGSYLTKVKLQM 300

Query: 301 LNVSDVSLAVIGHYGKAVTDLVLTDLKNVSEKGFWVMGNGHGLQKLKSFTISSCNGVTDM 360
           LNVS +SLAVIGHYG AVTDLVL  L+ V+EKGFWVMGN  GL+KLKS ++ SC G+TD+
Sbjct: 301 LNVSGLSLAVIGHYGAAVTDLVLHGLQGVNEKGFWVMGNAKGLKKLKSLSVMSCRGMTDV 360

Query: 361 GLESVGKGSPNLKH--------------FCLRKSARSLECLQLEECHRITQFGFFGVVLN 420
           GLE+VG G P+LKH                L KSA SLE L+LEECHRI QFG  G ++N
Sbjct: 361 GLEAVGNGCPDLKHVSLNKCLLVSGKGLVALAKSALSLESLKLEECHRINQFGLMGFLMN 420

Query: 421 CSATLKALSLISCLGIKDINSEFPIPA-SSGSLRSLTIRNCHGFGNRNLALLGKLCPQLQ 480
           C + LKA SL +CLGI D NSE  +P+ S  SLRSL+IR C GFG+ +LA LGK C QLQ
Sbjct: 421 CGSKLKAFSLANCLGISDFNSESSLPSPSCSSLRSLSIRCCPGFGDASLAFLGKFCHQLQ 480

Query: 481 NVDFSGLVGIDDCGFLAWLQNCQSGLVKINVNGCVNLTDKVVSSIIEHHGWSLKMLNLDG 540
           +V+  GL G+ D G    LQ+   GLVK+N++ C+N++D  VS+I   HG +L+ LNLDG
Sbjct: 481 DVELCGLNGVTDAGVRELLQSNNVGLVKVNLSECINVSDNTVSAISVCHGRTLESLNLDG 540

Query: 541 CKKITDASMTSIANNCPLLSDLDVSKCSITDSGIAALAHA-KRLNLQIFSISGCSFVSDK 600
           CK IT+AS+ ++A NC  ++DLD+S   ++D GI ALA +   LNLQ+ SI GCS ++DK
Sbjct: 541 CKNITNASLVAVAKNCYSVNDLDISNTLVSDHGIKALASSPNHLNLQVLSIGGCSSITDK 600

Query: 601 SLADLLNLGETLVGLNIQHCNAISSSTVDLLVEQLWRCDIL 626
           S A +  LG TL+GLNIQ C  ISSSTVD L+E LWRCDIL
Sbjct: 601 SKACIQKLGRTLLGLNIQRCGRISSSTVDTLLENLWRCDIL 622

BLAST of PI0027335 vs. ExPASy Swiss-Prot
Match: Q9C5D2 (F-box/LRR-repeat protein 4 OS=Arabidopsis thaliana OX=3702 GN=FBL4 PE=2 SV=1)

HSP 1 Score: 187.6 bits (475), Expect = 4.4e-46
Identity = 166/587 (28.28%), Postives = 278/587 (47.36%), Query Frame = 0

Query: 70  LPDECLFEIFRRLSGGKERSACATVSKRWLML----LSNIYSHELKSEDEVAS----KEV 129
           LP+E + EIFRRL     R AC+ V KRWL L     + +      S D+  S    + +
Sbjct: 11  LPEELILEIFRRLESKPNRDACSLVCKRWLSLERFSRTTLRIGASFSPDDFISLLSRRFL 70

Query: 130 EDIEIESDGYLSRSLEGKQATDLRLAAISVGTASRGGLGKLVIR-----GNNHVSKVTDL 189
               I  D  +S SL     +  R       + S     KL  +      N   S +TD 
Sbjct: 71  YITSIHVDERISVSLPSLSPSPKRKRGRDSSSPSSSKRKKLTDKTHSGAENVESSSLTDT 130

Query: 190 GLKAIARGCQSLRALSL-W--NLSS----------------------IRDEGLCEIAKAS 249
           GL A+A G   +  LSL W  N+SS                      + D+GL  + K  
Sbjct: 131 GLTALANGFPRIENLSLIWCPNVSSVGLCSLAQKCTSLKSLDLQGCYVGDQGLAAVGKFC 190

Query: 250 HQLEKLDLCRCPAVSDKALVEIARNCPK-LTDITIESCAKIGNESLRAIGQFCPKLKSIV 309
            QLE+L+L  C  ++D  ++++   C K L  I + + AKI + SL A+G  C KL  ++
Sbjct: 191 KQLEELNLRFCEGLTDVGVIDLVVGCSKSLKSIGVAASAKITDLSLEAVGSHC-KLLEVL 250

Query: 310 IKDCPLVGDQGIASLLSLNTCALNKVKLQALNVSDVSLAVIGHYGKAVTDLVLTDLKNVS 369
             D   + D+G+ + ++     L  +KLQ ++V+DV+ A +G    ++  L L   ++ +
Sbjct: 251 YLDSEYIHDKGLIA-VAQGCHRLKNLKLQCVSVTDVAFAAVGELCTSLERLALYSFQHFT 310

Query: 370 EKGFWVMGNGHGLQKLKSFTISSCNGVTDMGLESVGKGSPNLKHFCLRKSARSLECLQLE 429
           +KG   +G G   +KLK  T+S C  V+  GLE++  G             + LE +++ 
Sbjct: 311 DKGMRAIGKGS--KKLKDLTLSDCYFVSCKGLEAIAHG------------CKELERVEIN 370

Query: 430 ECHRITQFGFFGVVLNCSATLKALSLISCLGIKDINSEFPIPASSGSLRSLTIRNCHGFG 489
            CH I   G   +  +C   LK L+L+ C  I + ++   I     SL  L + +C G G
Sbjct: 371 GCHNIGTRGIEAIGKSC-PRLKELALLYCQRIGN-SALQEIGKGCKSLEILHLVDCSGIG 430

Query: 490 NRNLALLGKLCPQLQNVDFSGLVGIDDCGFLAWLQNCQSGLVKINVNGCVNLTDKVVSSI 549
           +  +  + K C  L+ +       I + G ++  ++C+S L ++++  C  + +K + +I
Sbjct: 431 DIAMCSIAKGCRNLKKLHIRRCYEIGNKGIISIGKHCKS-LTELSLRFCDKVGNKALIAI 490

Query: 550 IEHHGWSLKMLNLDGCKKITDASMTSIANNCPLLSDLDVSKC-SITDSGIAALAHAKRLN 609
               G SL+ LN+ GC +I+DA +T+IA  CP L+ LD+S   +I D  +A L     + 
Sbjct: 491 --GKGCSLQQLNVSGCNQISDAGITAIARGCPQLTHLDISVLQNIGDMPLAELGEGCPM- 550

Query: 610 LQIFSISGCSFVSDKSLADLLNLGETLVGLNIQHCNAISSSTVDLLV 617
           L+   +S C  ++D  L  L+   + L   ++ +C  I+S+ V  +V
Sbjct: 551 LKDLVLSHCHHITDNGLNHLVQKCKLLETCHMVYCPGITSAGVATVV 575

BLAST of PI0027335 vs. ExPASy Swiss-Prot
Match: Q8RWU5 (F-box/LRR-repeat protein 3 OS=Arabidopsis thaliana OX=3702 GN=FBL3 PE=2 SV=1)

HSP 1 Score: 135.6 bits (340), Expect = 2.0e-30
Identity = 135/475 (28.42%), Postives = 219/475 (46.11%), Query Frame = 0

Query: 141 ATDLRLAAISVGTASRGGLGKLVIRGNNHVSKVTDLGLKAIARGCQSLRALSLWNLSSIR 200
           AT++R A  +V   +R  L +L +        +TD+G+  IA GC+ L  +SL     + 
Sbjct: 135 ATEMRDADAAVVAEAR-SLERLKL---GRCKMLTDMGIGCIAVGCKKLNTVSLKWCVGVG 194

Query: 201 DEGLCEIAKASHQLEKLDLCRCPAVSDKALVEIARNCPKLTDITIESCAKIGNESLRAIG 260
           D G+  +A     +  LDL   P ++ K L +I +    L ++ +E C  + ++SL+++ 
Sbjct: 195 DLGVGLLAVKCKDIRTLDLSYLP-ITGKCLHDILK-LQHLEELLLEGCFGVDDDSLKSLR 254

Query: 261 QFCPKLKSIVIKDCPLVGDQGIASLLSLNTCALNKVKLQALNVSDVSLAVIGHYG---KA 320
             C  LK +    C  +  +G+ SLLS          LQ L++S  S  +   +    K 
Sbjct: 255 HDCKSLKKLDASSCQNLTHRGLTSLLS------GAGYLQRLDLSHCSSVISLDFASSLKK 314

Query: 321 VTDLVLTDLKNVSEKGFWVMGNGHGLQKLKSFTISSCNGVTDMGLESVGKGSPNLKHF-- 380
           V+ L    L   S     +   G     LK  ++S C  VTD GL S+     +L+    
Sbjct: 315 VSALQSIRLDGCSVTPDGLKAIGTLCNSLKEVSLSKCVSVTDEGLSSLVMKLKDLRKLDI 374

Query: 381 -CLRKSAR-----------SLECLQLEECHRITQFGFFGVVLNCSATLKALSL----ISC 440
            C RK +R            L  L++E C  +++  F+ +   C   L+ L L    I  
Sbjct: 375 TCCRKLSRVSITQIANSCPLLVSLKMESCSLVSREAFWLIGQKC-RLLEELDLTDNEIDD 434

Query: 441 LGIKDINSEFPIPASSGSLRSLTIRNCHGFGNRNLALLGKLCPQLQNVDFSGLVGIDDCG 500
            G+K I+S         SL SL +  C    ++ L+ +G  C  L+ +D    VGI D G
Sbjct: 435 EGLKSISSCL-------SLSSLKLGICLNITDKGLSYIGMGCSNLRELDLYRSVGITDVG 494

Query: 501 FLAWLQNCQSGLVKINVNGCVNLTDKVVSSIIEHHGWSLKMLNLDGCKKITDASMTSIAN 560
                Q C   L  IN++ C ++TDK + S+ +     L+     GC  IT   + +IA 
Sbjct: 495 ISTIAQGC-IHLETINISYCQDITDKSLVSLSKCS--LLQTFESRGCPNITSQGLAAIAV 554

Query: 561 NCPLLSDLDVSKC-SITDSGIAALAHAKRLNLQIFSISGCSFVSDKSLADLLNLG 594
            C  L+ +D+ KC SI D+G+ ALAH  + NL+  ++S  + V++  L  L N+G
Sbjct: 555 RCKRLAKVDLKKCPSINDAGLLALAHFSQ-NLKQINVSDTA-VTEVGLLSLANIG 584

BLAST of PI0027335 vs. ExPASy Swiss-Prot
Match: A6H779 (F-box/LRR-repeat protein 2 OS=Bos taurus OX=9913 GN=FBXL2 PE=2 SV=1)

HSP 1 Score: 104.8 bits (260), Expect = 3.7e-21
Identity = 66/230 (28.70%), Postives = 121/230 (52.61%), Query Frame = 0

Query: 172 KVTDLGLKAIARGCQSLRALSLWNLSSIRDEGLCEIAKASHQLEKLDLCRCPAVSDKALV 231
           ++T  G++A+ RGC+ LRAL L   + + DE L  I    H+L  L+L  C  V+D  +V
Sbjct: 168 QITKDGVEALVRGCRGLRALLLRGCTQLEDEALKHIQNYCHELVSLNLQSCSRVTDDGVV 227

Query: 232 EIARNCPKLTDITIESCAKIGNESLRAIGQFCPKLKSIVIKDCPLVGDQGIASLLSLNTC 291
           ++ R CP+L  + +  C  + + SL A+   CP+L+ +    C  + D G  +LL+ N  
Sbjct: 228 QLCRGCPRLQALCLSGCGSLTDASLTALALNCPRLQILEAARCSHLTDAGF-TLLARNCH 287

Query: 292 ALNKVKL-QALNVSDVSLAVIGHYGKAVTDLVLTDLKNVSEKGFWVMGNGH-GLQKLKSF 351
            L K+ L + + ++D +L  +  +   +  L L+  + +++ G   + N   G ++L+  
Sbjct: 288 DLEKMDLEECILITDRTLTQLSIHCPKLQALSLSHCELITDDGILHLSNSPCGHERLRVL 347

Query: 352 TISSCNGVTDMGLESVGKGSPNLKHFCLRKSARSLECLQLEECHRITQFG 400
            + +C  +TD+ LE       +L+H       R LE L+L +C ++T+ G
Sbjct: 348 ELDNCLLITDVALE-------HLEH------CRGLERLELYDCQQVTRAG 383

BLAST of PI0027335 vs. ExPASy TrEMBL
Match: A0A059VBN5 (EIN3-binding-Fbox protein 1 OS=Cucumis sativus OX=3659 GN=Csa_3G878200 PE=2 SV=1)

HSP 1 Score: 1196.4 bits (3094), Expect = 0.0e+00
Identity = 603/640 (94.22%), Postives = 617/640 (96.41%), Query Frame = 0

Query: 1   MSKLFAFSGSEDFCPGGSIYPNPKDSSLFLSLPHHVDVYFPPRKRSRITAPFVFGGEEVE 60
           MSKLFAFSGSEDFC GGSIYPNPKDSSLFLSLPHHVDVYFPPRKRSRITAPFVFGGEEVE
Sbjct: 1   MSKLFAFSGSEDFCTGGSIYPNPKDSSLFLSLPHHVDVYFPPRKRSRITAPFVFGGEEVE 60

Query: 61  SKANVSIEILPDECLFEIFRRLSGGKERSACATVSKRWLMLLSNIYSHELKSEDEVASKE 120
           SKANVSIEILPDECLFEIFRRLS GKERSACATVSKRWLMLLSNI SHELKSEDEVASKE
Sbjct: 61  SKANVSIEILPDECLFEIFRRLSDGKERSACATVSKRWLMLLSNISSHELKSEDEVASKE 120

Query: 121 VEDIEIESDGYLSRSLEGKQATDLRLAAISVGTASRGGLGKLVIRGNNHVSKVTDLGLKA 180
           VEDIEIESDGYLSRSLEGK+ATDLRLAAISVGTASRGGLGKLVIRGNNHVSKVTDLGLKA
Sbjct: 121 VEDIEIESDGYLSRSLEGKKATDLRLAAISVGTASRGGLGKLVIRGNNHVSKVTDLGLKA 180

Query: 181 IARGCQSLRALSLWNLSSIRDEGLCEIAKASHQLEKLDLCRCPAVSDKALVEIARNCPKL 240
           IARGCQSLRALSLWNLSSIRDEGLCEIAKASHQLEKLDLCRCPAVSDKA+VEIARNCPKL
Sbjct: 181 IARGCQSLRALSLWNLSSIRDEGLCEIAKASHQLEKLDLCRCPAVSDKAVVEIARNCPKL 240

Query: 241 TDITIESCAKIGNESLRAIGQFCPKLKSIVIKDCPLVGDQGIASLLSLNTCALNKVKLQA 300
           TDITIESCAKIGNES+RAIGQFCPKLKSIVIKDCPLVGDQGIASLLSLNTCALNKVKLQA
Sbjct: 241 TDITIESCAKIGNESMRAIGQFCPKLKSIVIKDCPLVGDQGIASLLSLNTCALNKVKLQA 300

Query: 301 LNVSDVSLAVIGHYGKAVTDLVLTDLKNVSEKGFWVMGNGHGLQKLKSFTISSCNGVTDM 360
           LNVSDVSLAVIGHYGKAVTDLVLTDLKNVSEKGFWVMGNGHGLQKLKSFTISSCNGVTDM
Sbjct: 301 LNVSDVSLAVIGHYGKAVTDLVLTDLKNVSEKGFWVMGNGHGLQKLKSFTISSCNGVTDM 360

Query: 361 GLESVGKGSPNLKHFCLR--------------KSARSLECLQLEECHRITQFGFFGVVLN 420
           GLESVGKGSPNLKHFCLR              K+ARSLECLQLEECHRITQFGFFGVVLN
Sbjct: 361 GLESVGKGSPNLKHFCLRKCSFLSDNGLVSFAKAARSLECLQLEECHRITQFGFFGVVLN 420

Query: 421 CSATLKALSLISCLGIKDINSEFPIPASSGSLRSLTIRNCHGFGNRNLALLGKLCPQLQN 480
           CSA+LKALSLISCLGIKDINSE PIPASS SLRSLTIRNCHGFGNRNLALLGKLCPQLQN
Sbjct: 421 CSASLKALSLISCLGIKDINSELPIPASSVSLRSLTIRNCHGFGNRNLALLGKLCPQLQN 480

Query: 481 VDFSGLVGIDDCGFLAWLQNCQSGLVKINVNGCVNLTDKVVSSIIEHHGWSLKMLNLDGC 540
           VDFSGLVGI+DCGFLAWLQNCQ GLVKIN+NGCVNLTD+VVSS++EHHG +LKMLNLD C
Sbjct: 481 VDFSGLVGIEDCGFLAWLQNCQLGLVKINLNGCVNLTDEVVSSLMEHHGSTLKMLNLDSC 540

Query: 541 KKITDASMTSIANNCPLLSDLDVSKCSITDSGIAALAHAKRLNLQIFSISGCSFVSDKSL 600
           KKITDASMTSIANNCPLLSDLDVSKCSITDSGIA LAHAK+LNLQIFSISGCSFVS+KSL
Sbjct: 541 KKITDASMTSIANNCPLLSDLDVSKCSITDSGIATLAHAKQLNLQIFSISGCSFVSEKSL 600

Query: 601 ADLLNLGETLVGLNIQHCNAISSSTVDLLVEQLWRCDILS 627
           ADL+NLGETLVGLNIQHCNAISSSTVDLLVEQLWRCDILS
Sbjct: 601 ADLINLGETLVGLNIQHCNAISSSTVDLLVEQLWRCDILS 640

BLAST of PI0027335 vs. ExPASy TrEMBL
Match: A0A5A7TB22 (EIN3-binding F-box protein 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold92G003680 PE=4 SV=1)

HSP 1 Score: 1191.4 bits (3081), Expect = 0.0e+00
Identity = 601/640 (93.91%), Postives = 614/640 (95.94%), Query Frame = 0

Query: 1   MSKLFAFSGSEDFCPGGSIYPNPKDSSLFLSLPHHVDVYFPPRKRSRITAPFVFGGEEVE 60
           MSKLFAFSGSEDFC GGSIYPNPKDSSLFLSLPHHVDVYFPPRKRSRITAPFVFGGEEVE
Sbjct: 1   MSKLFAFSGSEDFCTGGSIYPNPKDSSLFLSLPHHVDVYFPPRKRSRITAPFVFGGEEVE 60

Query: 61  SKANVSIEILPDECLFEIFRRLSGGKERSACATVSKRWLMLLSNIYSHELKSEDEVASKE 120
           SKANVSIEILPDECLFEIFRRLSGGKERSACATVSKRWLMLLSNI SHELKSED VASKE
Sbjct: 61  SKANVSIEILPDECLFEIFRRLSGGKERSACATVSKRWLMLLSNISSHELKSEDVVASKE 120

Query: 121 VEDIEIESDGYLSRSLEGKQATDLRLAAISVGTASRGGLGKLVIRGNNHVSKVTDLGLKA 180
           VEDIEIESDGYLSRSLEGK+ATDLRLAAISVGTA RGGLGKLVIRGNNHVSKVTDLGLKA
Sbjct: 121 VEDIEIESDGYLSRSLEGKKATDLRLAAISVGTAGRGGLGKLVIRGNNHVSKVTDLGLKA 180

Query: 181 IARGCQSLRALSLWNLSSIRDEGLCEIAKASHQLEKLDLCRCPAVSDKALVEIARNCPKL 240
           IARGCQSLRALSLWNLSSIRD GLCEIAKASHQLEKLDLCRCPAVSDKA+VEIARNCPKL
Sbjct: 181 IARGCQSLRALSLWNLSSIRDGGLCEIAKASHQLEKLDLCRCPAVSDKAVVEIARNCPKL 240

Query: 241 TDITIESCAKIGNESLRAIGQFCPKLKSIVIKDCPLVGDQGIASLLSLNTCALNKVKLQA 300
           TDITIESCA+IGNESLRAIGQFC KLKSIVIKDCPLVGDQGIASLLSLNTCALNKVKLQA
Sbjct: 241 TDITIESCAQIGNESLRAIGQFCTKLKSIVIKDCPLVGDQGIASLLSLNTCALNKVKLQA 300

Query: 301 LNVSDVSLAVIGHYGKAVTDLVLTDLKNVSEKGFWVMGNGHGLQKLKSFTISSCNGVTDM 360
           LNVSDVSLAVIGHYGKAVTDLVLTDLKNVSEKGFWVMGNGHGLQKLKSFTISSCNGVTDM
Sbjct: 301 LNVSDVSLAVIGHYGKAVTDLVLTDLKNVSEKGFWVMGNGHGLQKLKSFTISSCNGVTDM 360

Query: 361 GLESVGKGSPNLKHFCLR--------------KSARSLECLQLEECHRITQFGFFGVVLN 420
           GLESVGKGSPNLKHFCLR              K+ARSLECLQLEECHRITQFGFFGVVLN
Sbjct: 361 GLESVGKGSPNLKHFCLRKCSFLSDNGLVSFAKAARSLECLQLEECHRITQFGFFGVVLN 420

Query: 421 CSATLKALSLISCLGIKDINSEFPIPASSGSLRSLTIRNCHGFGNRNLALLGKLCPQLQN 480
           CSA+LKALSLISCLGIKDINSE PIPASSGSLRSLTIRNCHGFGNRNLALLGKLCPQLQN
Sbjct: 421 CSASLKALSLISCLGIKDINSELPIPASSGSLRSLTIRNCHGFGNRNLALLGKLCPQLQN 480

Query: 481 VDFSGLVGIDDCGFLAWLQNCQSGLVKINVNGCVNLTDKVVSSIIEHHGWSLKMLNLDGC 540
           VDFSGLVGIDDCGFLAWLQNCQSGLVKIN++GCVNLTD+V+SSIIEHHGW+LK+LNLD C
Sbjct: 481 VDFSGLVGIDDCGFLAWLQNCQSGLVKINLSGCVNLTDEVISSIIEHHGWTLKVLNLDSC 540

Query: 541 KKITDASMTSIANNCPLLSDLDVSKCSITDSGIAALAHAKRLNLQIFSISGCSFVSDKSL 600
           KKITDASMTSIANNCPLLSDLDVSKCSITDSGIA LA AK+ NLQIFSISGCSFVSDKSL
Sbjct: 541 KKITDASMTSIANNCPLLSDLDVSKCSITDSGIATLACAKQPNLQIFSISGCSFVSDKSL 600

Query: 601 ADLLNLGETLVGLNIQHCNAISSSTVDLLVEQLWRCDILS 627
           ADL+ LGETLVGLNIQHCNAISSSTVDLLVEQLWRCDILS
Sbjct: 601 ADLIKLGETLVGLNIQHCNAISSSTVDLLVEQLWRCDILS 640

BLAST of PI0027335 vs. ExPASy TrEMBL
Match: A0A1S4E5P7 (EIN3-binding F-box protein 1 OS=Cucumis melo OX=3656 GN=LOC103503821 PE=4 SV=1)

HSP 1 Score: 1191.4 bits (3081), Expect = 0.0e+00
Identity = 601/640 (93.91%), Postives = 614/640 (95.94%), Query Frame = 0

Query: 1   MSKLFAFSGSEDFCPGGSIYPNPKDSSLFLSLPHHVDVYFPPRKRSRITAPFVFGGEEVE 60
           MSKLFAFSGSEDFC GGSIYPNPKDSSLFLSLPHHVDVYFPPRKRSRITAPFVFGGEEVE
Sbjct: 1   MSKLFAFSGSEDFCTGGSIYPNPKDSSLFLSLPHHVDVYFPPRKRSRITAPFVFGGEEVE 60

Query: 61  SKANVSIEILPDECLFEIFRRLSGGKERSACATVSKRWLMLLSNIYSHELKSEDEVASKE 120
           SKANVSIEILPDECLFEIFRRLSGGKERSACATVSKRWLMLLSNI SHELKSED VASKE
Sbjct: 61  SKANVSIEILPDECLFEIFRRLSGGKERSACATVSKRWLMLLSNISSHELKSEDVVASKE 120

Query: 121 VEDIEIESDGYLSRSLEGKQATDLRLAAISVGTASRGGLGKLVIRGNNHVSKVTDLGLKA 180
           VEDIEIESDGYLSRSLEGK+ATDLRLAAISVGTA RGGLGKLVIRGNNHVSKVTDLGLKA
Sbjct: 121 VEDIEIESDGYLSRSLEGKKATDLRLAAISVGTAGRGGLGKLVIRGNNHVSKVTDLGLKA 180

Query: 181 IARGCQSLRALSLWNLSSIRDEGLCEIAKASHQLEKLDLCRCPAVSDKALVEIARNCPKL 240
           IARGCQSLRALSLWNLSSIRD GLCEIAKASHQLEKLDLCRCPAVSDKA+VEIARNCPKL
Sbjct: 181 IARGCQSLRALSLWNLSSIRDGGLCEIAKASHQLEKLDLCRCPAVSDKAVVEIARNCPKL 240

Query: 241 TDITIESCAKIGNESLRAIGQFCPKLKSIVIKDCPLVGDQGIASLLSLNTCALNKVKLQA 300
           TDITIESCA+IGNESLRAIGQFC KLKSIVIKDCPLVGDQGIASLLSLNTCALNKVKLQA
Sbjct: 241 TDITIESCAQIGNESLRAIGQFCTKLKSIVIKDCPLVGDQGIASLLSLNTCALNKVKLQA 300

Query: 301 LNVSDVSLAVIGHYGKAVTDLVLTDLKNVSEKGFWVMGNGHGLQKLKSFTISSCNGVTDM 360
           LNVSDVSLAVIGHYGKAVTDLVLTDLKNVSEKGFWVMGNGHGLQKLKSFTISSCNGVTDM
Sbjct: 301 LNVSDVSLAVIGHYGKAVTDLVLTDLKNVSEKGFWVMGNGHGLQKLKSFTISSCNGVTDM 360

Query: 361 GLESVGKGSPNLKHFCLR--------------KSARSLECLQLEECHRITQFGFFGVVLN 420
           GLESVGKGSPNLKHFCLR              K+ARSLECLQLEECHRITQFGFFGVVLN
Sbjct: 361 GLESVGKGSPNLKHFCLRKCSFLSDNGLVSFAKAARSLECLQLEECHRITQFGFFGVVLN 420

Query: 421 CSATLKALSLISCLGIKDINSEFPIPASSGSLRSLTIRNCHGFGNRNLALLGKLCPQLQN 480
           CSA+LKALSLISCLGIKDINSE PIPASSGSLRSLTIRNCHGFGNRNLALLGKLCPQLQN
Sbjct: 421 CSASLKALSLISCLGIKDINSELPIPASSGSLRSLTIRNCHGFGNRNLALLGKLCPQLQN 480

Query: 481 VDFSGLVGIDDCGFLAWLQNCQSGLVKINVNGCVNLTDKVVSSIIEHHGWSLKMLNLDGC 540
           VDFSGLVGIDDCGFLAWLQNCQSGLVKIN++GCVNLTD+V+SSIIEHHGW+LK+LNLD C
Sbjct: 481 VDFSGLVGIDDCGFLAWLQNCQSGLVKINLSGCVNLTDEVISSIIEHHGWTLKVLNLDSC 540

Query: 541 KKITDASMTSIANNCPLLSDLDVSKCSITDSGIAALAHAKRLNLQIFSISGCSFVSDKSL 600
           KKITDASMTSIANNCPLLSDLDVSKCSITDSGIA LA AK+ NLQIFSISGCSFVSDKSL
Sbjct: 541 KKITDASMTSIANNCPLLSDLDVSKCSITDSGIATLACAKQPNLQIFSISGCSFVSDKSL 600

Query: 601 ADLLNLGETLVGLNIQHCNAISSSTVDLLVEQLWRCDILS 627
           ADL+ LGETLVGLNIQHCNAISSSTVDLLVEQLWRCDILS
Sbjct: 601 ADLIKLGETLVGLNIQHCNAISSSTVDLLVEQLWRCDILS 640

BLAST of PI0027335 vs. ExPASy TrEMBL
Match: A0A5D3E633 (EIN3-binding F-box protein 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold455G007450 PE=4 SV=1)

HSP 1 Score: 1176.4 bits (3042), Expect = 0.0e+00
Identity = 593/632 (93.83%), Postives = 606/632 (95.89%), Query Frame = 0

Query: 9   GSEDFCPGGSIYPNPKDSSLFLSLPHHVDVYFPPRKRSRITAPFVFGGEEVESKANVSIE 68
           GSEDFC GGSIYPNPKDSSLFLSLPHHVDVYFPPRKRSRITAPFVFGGEEVESKANVSIE
Sbjct: 15  GSEDFCTGGSIYPNPKDSSLFLSLPHHVDVYFPPRKRSRITAPFVFGGEEVESKANVSIE 74

Query: 69  ILPDECLFEIFRRLSGGKERSACATVSKRWLMLLSNIYSHELKSEDEVASKEVEDIEIES 128
           ILPDECLFEIFRRLSGGKERSACATVSKRWLMLLSNI SHELKSED VASKEVEDIEIES
Sbjct: 75  ILPDECLFEIFRRLSGGKERSACATVSKRWLMLLSNISSHELKSEDVVASKEVEDIEIES 134

Query: 129 DGYLSRSLEGKQATDLRLAAISVGTASRGGLGKLVIRGNNHVSKVTDLGLKAIARGCQSL 188
           DGYLSRSLEGK+ATDLRLAAISVGTA RGGLGKLVIRGNNHVSKVTDLGLKAIARGCQSL
Sbjct: 135 DGYLSRSLEGKKATDLRLAAISVGTAGRGGLGKLVIRGNNHVSKVTDLGLKAIARGCQSL 194

Query: 189 RALSLWNLSSIRDEGLCEIAKASHQLEKLDLCRCPAVSDKALVEIARNCPKLTDITIESC 248
           RALSLWNLSSIRD GLCEIAKASHQLEKLDLCRCPAVSDKA+VEIARNCPKLTDITIESC
Sbjct: 195 RALSLWNLSSIRDGGLCEIAKASHQLEKLDLCRCPAVSDKAVVEIARNCPKLTDITIESC 254

Query: 249 AKIGNESLRAIGQFCPKLKSIVIKDCPLVGDQGIASLLSLNTCALNKVKLQALNVSDVSL 308
           A+IGNESLRAIGQFC KLKSIVIKDCPLVGDQGIASLLSLNTCALNKVKLQALNVSDVSL
Sbjct: 255 AQIGNESLRAIGQFCTKLKSIVIKDCPLVGDQGIASLLSLNTCALNKVKLQALNVSDVSL 314

Query: 309 AVIGHYGKAVTDLVLTDLKNVSEKGFWVMGNGHGLQKLKSFTISSCNGVTDMGLESVGKG 368
           AVIGHYGKAVTDLVLTDLKNVSEKGFWVMGNGHGLQKLKSFTISSCNGVTDMGLESVGKG
Sbjct: 315 AVIGHYGKAVTDLVLTDLKNVSEKGFWVMGNGHGLQKLKSFTISSCNGVTDMGLESVGKG 374

Query: 369 SPNLKHFCLR--------------KSARSLECLQLEECHRITQFGFFGVVLNCSATLKAL 428
           SPNLKHFCLR              K+ARSLECLQLEECHRITQFGFFGVVLNCSA+LKAL
Sbjct: 375 SPNLKHFCLRKCSFLSDNGLVSFAKAARSLECLQLEECHRITQFGFFGVVLNCSASLKAL 434

Query: 429 SLISCLGIKDINSEFPIPASSGSLRSLTIRNCHGFGNRNLALLGKLCPQLQNVDFSGLVG 488
           SLISCLGIKDINSE PIPASSGSLRSLTIRNCHGFGNRNLALLGKLCPQLQNVDFSGLVG
Sbjct: 435 SLISCLGIKDINSELPIPASSGSLRSLTIRNCHGFGNRNLALLGKLCPQLQNVDFSGLVG 494

Query: 489 IDDCGFLAWLQNCQSGLVKINVNGCVNLTDKVVSSIIEHHGWSLKMLNLDGCKKITDASM 548
           IDDCGFLAWLQNCQSGLVKIN++GCVNLTD+V+SSIIEHHGW+LK+LNLD CKKITDASM
Sbjct: 495 IDDCGFLAWLQNCQSGLVKINLSGCVNLTDEVISSIIEHHGWTLKVLNLDSCKKITDASM 554

Query: 549 TSIANNCPLLSDLDVSKCSITDSGIAALAHAKRLNLQIFSISGCSFVSDKSLADLLNLGE 608
           TSIANNCPLLSDLDVSKCSITDSGIA LA AK+ NLQIFSISGCSFVSDKSLADL+ LGE
Sbjct: 555 TSIANNCPLLSDLDVSKCSITDSGIATLACAKQPNLQIFSISGCSFVSDKSLADLIKLGE 614

Query: 609 TLVGLNIQHCNAISSSTVDLLVEQLWRCDILS 627
           TLVGLNIQHCNAISSSTVDLLVEQLWRCDILS
Sbjct: 615 TLVGLNIQHCNAISSSTVDLLVEQLWRCDILS 646

BLAST of PI0027335 vs. ExPASy TrEMBL
Match: A0A6J1BUE6 (EIN3-binding F-box protein 1 OS=Momordica charantia OX=3673 GN=LOC111005790 PE=4 SV=1)

HSP 1 Score: 1136.3 bits (2938), Expect = 0.0e+00
Identity = 564/641 (87.99%), Postives = 603/641 (94.07%), Query Frame = 0

Query: 1   MSKLFAFSGSEDFCPGGSIYPNPKDSSLFLSLPHHVDVYFPPRKRSRITAPFVFGGEEVE 60
           MSKLFA+SGSEDFCPGGSIYP+ KDSSLFLSLPHHVDVYFPPRKRSRITAPFVFGGE VE
Sbjct: 1   MSKLFAYSGSEDFCPGGSIYPSSKDSSLFLSLPHHVDVYFPPRKRSRITAPFVFGGEGVE 60

Query: 61  SKANVSIEILPDECLFEIFRRLSGGKERSACATVSKRWLMLLSNIYSHELKSEDEVASKE 120
           SKANVSIE+LPDECLFEIFRRL GGKERSACA VSK+WLMLLSNI  HELKS DEVASKE
Sbjct: 61  SKANVSIEVLPDECLFEIFRRLPGGKERSACAGVSKKWLMLLSNISCHELKSGDEVASKE 120

Query: 121 VEDIEIESDGYLSRSLEGKQATDLRLAAISVGTASRGGLGKLVIRGNNHVSKVTDLGLKA 180
           VED+EIESDGYLSRSLEGK+ATDLRLAAISVGTASRGGLGKLVIRGNNH SKVTDLGL+A
Sbjct: 121 VEDMEIESDGYLSRSLEGKKATDLRLAAISVGTASRGGLGKLVIRGNNHASKVTDLGLRA 180

Query: 181 IARGCQSLRALSLWNLSSIRDEGLCEIAKASHQLEKLDLCRCPAVSDKALVEIARNCPKL 240
           +ARGCQSLRALSLWNLSSI DEGLCEIA  SHQLEKLDLCRCPA+SDKA++ IARNCPKL
Sbjct: 181 VARGCQSLRALSLWNLSSIGDEGLCEIASVSHQLEKLDLCRCPAISDKAVIAIARNCPKL 240

Query: 241 TDITIESCAKIGNESLRAIGQFCPKLKSIVIKDCPLVGDQGIASLLSLNTCALNKVKLQA 300
           TDITIESCAKIGNESLRAIGQ CPKLKSIVIKDCPLVGDQGIASLLSLNTCALNKVKLQA
Sbjct: 241 TDITIESCAKIGNESLRAIGQCCPKLKSIVIKDCPLVGDQGIASLLSLNTCALNKVKLQA 300

Query: 301 LNVSDVSLAVIGHYGKAVTDLVLTDLKNVSEKGFWVMGNGHGLQKLKSFTISSCNGVTDM 360
           LN+SDVSLAVIGHYGK+VTDLVL+DLKNVSEKGFWVMGNGHGLQKLKSFTISSCNGVTDM
Sbjct: 301 LNISDVSLAVIGHYGKSVTDLVLSDLKNVSEKGFWVMGNGHGLQKLKSFTISSCNGVTDM 360

Query: 361 GLESVGKGSPNLKHFCLR--------------KSARSLECLQLEECHRITQFGFFGVVLN 420
           GLESVGKGSPNLKHFCLR              K+ARSLE LQLEECHRITQFGFFGV+LN
Sbjct: 361 GLESVGKGSPNLKHFCLRKCSFLSDSGLVSFAKAARSLENLQLEECHRITQFGFFGVLLN 420

Query: 421 CSATLKALSLISCLGIKDINSEFPIPASSG-SLRSLTIRNCHGFGNRNLALLGKLCPQLQ 480
           CS +LKALSL+SCLGIKD++SE PIP+SSG SLRSLTIRNCHGFGN+NLA LGKLCPQLQ
Sbjct: 421 CSTSLKALSLVSCLGIKDMSSESPIPSSSGSSLRSLTIRNCHGFGNKNLASLGKLCPQLQ 480

Query: 481 NVDFSGLVGIDDCGFLAWLQNCQSGLVKINVNGCVNLTDKVVSSIIEHHGWSLKMLNLDG 540
           +VDFSGLVGIDDCGFLAWLQNCQSGLVK+N++GCVNLTDK VSSIIEHHGW+L++LNLDG
Sbjct: 481 HVDFSGLVGIDDCGFLAWLQNCQSGLVKLNISGCVNLTDKAVSSIIEHHGWTLELLNLDG 540

Query: 541 CKKITDASMTSIANNCPLLSDLDVSKCSITDSGIAALAHAKRLNLQIFSISGCSFVSDKS 600
           C+KITDAS+ SIANNCPLLSDLD+SKCSI DSGIAALAHAK+L+LQIFS+SGCS VSDKS
Sbjct: 541 CRKITDASLVSIANNCPLLSDLDISKCSIMDSGIAALAHAKQLSLQIFSVSGCSLVSDKS 600

Query: 601 LADLLNLGETLVGLNIQHCNAISSSTVDLLVEQLWRCDILS 627
           L +L+ LGETL+GLNIQHCN+ISSSTVDLLVEQLWRCDILS
Sbjct: 601 LENLIKLGETLMGLNIQHCNSISSSTVDLLVEQLWRCDILS 641

BLAST of PI0027335 vs. NCBI nr
Match: NP_001281254.1 (EIN3-binding F-box protein 1-like [Cucumis sativus] >AHZ89385.1 EIN3-binding-Fbox protein 1 [Cucumis sativus] >KGN60100.1 hypothetical protein Csa_001541 [Cucumis sativus])

HSP 1 Score: 1196.4 bits (3094), Expect = 0.0e+00
Identity = 603/640 (94.22%), Postives = 617/640 (96.41%), Query Frame = 0

Query: 1   MSKLFAFSGSEDFCPGGSIYPNPKDSSLFLSLPHHVDVYFPPRKRSRITAPFVFGGEEVE 60
           MSKLFAFSGSEDFC GGSIYPNPKDSSLFLSLPHHVDVYFPPRKRSRITAPFVFGGEEVE
Sbjct: 1   MSKLFAFSGSEDFCTGGSIYPNPKDSSLFLSLPHHVDVYFPPRKRSRITAPFVFGGEEVE 60

Query: 61  SKANVSIEILPDECLFEIFRRLSGGKERSACATVSKRWLMLLSNIYSHELKSEDEVASKE 120
           SKANVSIEILPDECLFEIFRRLS GKERSACATVSKRWLMLLSNI SHELKSEDEVASKE
Sbjct: 61  SKANVSIEILPDECLFEIFRRLSDGKERSACATVSKRWLMLLSNISSHELKSEDEVASKE 120

Query: 121 VEDIEIESDGYLSRSLEGKQATDLRLAAISVGTASRGGLGKLVIRGNNHVSKVTDLGLKA 180
           VEDIEIESDGYLSRSLEGK+ATDLRLAAISVGTASRGGLGKLVIRGNNHVSKVTDLGLKA
Sbjct: 121 VEDIEIESDGYLSRSLEGKKATDLRLAAISVGTASRGGLGKLVIRGNNHVSKVTDLGLKA 180

Query: 181 IARGCQSLRALSLWNLSSIRDEGLCEIAKASHQLEKLDLCRCPAVSDKALVEIARNCPKL 240
           IARGCQSLRALSLWNLSSIRDEGLCEIAKASHQLEKLDLCRCPAVSDKA+VEIARNCPKL
Sbjct: 181 IARGCQSLRALSLWNLSSIRDEGLCEIAKASHQLEKLDLCRCPAVSDKAVVEIARNCPKL 240

Query: 241 TDITIESCAKIGNESLRAIGQFCPKLKSIVIKDCPLVGDQGIASLLSLNTCALNKVKLQA 300
           TDITIESCAKIGNES+RAIGQFCPKLKSIVIKDCPLVGDQGIASLLSLNTCALNKVKLQA
Sbjct: 241 TDITIESCAKIGNESMRAIGQFCPKLKSIVIKDCPLVGDQGIASLLSLNTCALNKVKLQA 300

Query: 301 LNVSDVSLAVIGHYGKAVTDLVLTDLKNVSEKGFWVMGNGHGLQKLKSFTISSCNGVTDM 360
           LNVSDVSLAVIGHYGKAVTDLVLTDLKNVSEKGFWVMGNGHGLQKLKSFTISSCNGVTDM
Sbjct: 301 LNVSDVSLAVIGHYGKAVTDLVLTDLKNVSEKGFWVMGNGHGLQKLKSFTISSCNGVTDM 360

Query: 361 GLESVGKGSPNLKHFCLR--------------KSARSLECLQLEECHRITQFGFFGVVLN 420
           GLESVGKGSPNLKHFCLR              K+ARSLECLQLEECHRITQFGFFGVVLN
Sbjct: 361 GLESVGKGSPNLKHFCLRKCSFLSDNGLVSFAKAARSLECLQLEECHRITQFGFFGVVLN 420

Query: 421 CSATLKALSLISCLGIKDINSEFPIPASSGSLRSLTIRNCHGFGNRNLALLGKLCPQLQN 480
           CSA+LKALSLISCLGIKDINSE PIPASS SLRSLTIRNCHGFGNRNLALLGKLCPQLQN
Sbjct: 421 CSASLKALSLISCLGIKDINSELPIPASSVSLRSLTIRNCHGFGNRNLALLGKLCPQLQN 480

Query: 481 VDFSGLVGIDDCGFLAWLQNCQSGLVKINVNGCVNLTDKVVSSIIEHHGWSLKMLNLDGC 540
           VDFSGLVGI+DCGFLAWLQNCQ GLVKIN+NGCVNLTD+VVSS++EHHG +LKMLNLD C
Sbjct: 481 VDFSGLVGIEDCGFLAWLQNCQLGLVKINLNGCVNLTDEVVSSLMEHHGSTLKMLNLDSC 540

Query: 541 KKITDASMTSIANNCPLLSDLDVSKCSITDSGIAALAHAKRLNLQIFSISGCSFVSDKSL 600
           KKITDASMTSIANNCPLLSDLDVSKCSITDSGIA LAHAK+LNLQIFSISGCSFVS+KSL
Sbjct: 541 KKITDASMTSIANNCPLLSDLDVSKCSITDSGIATLAHAKQLNLQIFSISGCSFVSEKSL 600

Query: 601 ADLLNLGETLVGLNIQHCNAISSSTVDLLVEQLWRCDILS 627
           ADL+NLGETLVGLNIQHCNAISSSTVDLLVEQLWRCDILS
Sbjct: 601 ADLINLGETLVGLNIQHCNAISSSTVDLLVEQLWRCDILS 640

BLAST of PI0027335 vs. NCBI nr
Match: XP_016903542.1 (PREDICTED: EIN3-binding F-box protein 1 [Cucumis melo] >KAA0038811.1 EIN3-binding F-box protein 1 [Cucumis melo var. makuwa])

HSP 1 Score: 1191.4 bits (3081), Expect = 0.0e+00
Identity = 601/640 (93.91%), Postives = 614/640 (95.94%), Query Frame = 0

Query: 1   MSKLFAFSGSEDFCPGGSIYPNPKDSSLFLSLPHHVDVYFPPRKRSRITAPFVFGGEEVE 60
           MSKLFAFSGSEDFC GGSIYPNPKDSSLFLSLPHHVDVYFPPRKRSRITAPFVFGGEEVE
Sbjct: 1   MSKLFAFSGSEDFCTGGSIYPNPKDSSLFLSLPHHVDVYFPPRKRSRITAPFVFGGEEVE 60

Query: 61  SKANVSIEILPDECLFEIFRRLSGGKERSACATVSKRWLMLLSNIYSHELKSEDEVASKE 120
           SKANVSIEILPDECLFEIFRRLSGGKERSACATVSKRWLMLLSNI SHELKSED VASKE
Sbjct: 61  SKANVSIEILPDECLFEIFRRLSGGKERSACATVSKRWLMLLSNISSHELKSEDVVASKE 120

Query: 121 VEDIEIESDGYLSRSLEGKQATDLRLAAISVGTASRGGLGKLVIRGNNHVSKVTDLGLKA 180
           VEDIEIESDGYLSRSLEGK+ATDLRLAAISVGTA RGGLGKLVIRGNNHVSKVTDLGLKA
Sbjct: 121 VEDIEIESDGYLSRSLEGKKATDLRLAAISVGTAGRGGLGKLVIRGNNHVSKVTDLGLKA 180

Query: 181 IARGCQSLRALSLWNLSSIRDEGLCEIAKASHQLEKLDLCRCPAVSDKALVEIARNCPKL 240
           IARGCQSLRALSLWNLSSIRD GLCEIAKASHQLEKLDLCRCPAVSDKA+VEIARNCPKL
Sbjct: 181 IARGCQSLRALSLWNLSSIRDGGLCEIAKASHQLEKLDLCRCPAVSDKAVVEIARNCPKL 240

Query: 241 TDITIESCAKIGNESLRAIGQFCPKLKSIVIKDCPLVGDQGIASLLSLNTCALNKVKLQA 300
           TDITIESCA+IGNESLRAIGQFC KLKSIVIKDCPLVGDQGIASLLSLNTCALNKVKLQA
Sbjct: 241 TDITIESCAQIGNESLRAIGQFCTKLKSIVIKDCPLVGDQGIASLLSLNTCALNKVKLQA 300

Query: 301 LNVSDVSLAVIGHYGKAVTDLVLTDLKNVSEKGFWVMGNGHGLQKLKSFTISSCNGVTDM 360
           LNVSDVSLAVIGHYGKAVTDLVLTDLKNVSEKGFWVMGNGHGLQKLKSFTISSCNGVTDM
Sbjct: 301 LNVSDVSLAVIGHYGKAVTDLVLTDLKNVSEKGFWVMGNGHGLQKLKSFTISSCNGVTDM 360

Query: 361 GLESVGKGSPNLKHFCLR--------------KSARSLECLQLEECHRITQFGFFGVVLN 420
           GLESVGKGSPNLKHFCLR              K+ARSLECLQLEECHRITQFGFFGVVLN
Sbjct: 361 GLESVGKGSPNLKHFCLRKCSFLSDNGLVSFAKAARSLECLQLEECHRITQFGFFGVVLN 420

Query: 421 CSATLKALSLISCLGIKDINSEFPIPASSGSLRSLTIRNCHGFGNRNLALLGKLCPQLQN 480
           CSA+LKALSLISCLGIKDINSE PIPASSGSLRSLTIRNCHGFGNRNLALLGKLCPQLQN
Sbjct: 421 CSASLKALSLISCLGIKDINSELPIPASSGSLRSLTIRNCHGFGNRNLALLGKLCPQLQN 480

Query: 481 VDFSGLVGIDDCGFLAWLQNCQSGLVKINVNGCVNLTDKVVSSIIEHHGWSLKMLNLDGC 540
           VDFSGLVGIDDCGFLAWLQNCQSGLVKIN++GCVNLTD+V+SSIIEHHGW+LK+LNLD C
Sbjct: 481 VDFSGLVGIDDCGFLAWLQNCQSGLVKINLSGCVNLTDEVISSIIEHHGWTLKVLNLDSC 540

Query: 541 KKITDASMTSIANNCPLLSDLDVSKCSITDSGIAALAHAKRLNLQIFSISGCSFVSDKSL 600
           KKITDASMTSIANNCPLLSDLDVSKCSITDSGIA LA AK+ NLQIFSISGCSFVSDKSL
Sbjct: 541 KKITDASMTSIANNCPLLSDLDVSKCSITDSGIATLACAKQPNLQIFSISGCSFVSDKSL 600

Query: 601 ADLLNLGETLVGLNIQHCNAISSSTVDLLVEQLWRCDILS 627
           ADL+ LGETLVGLNIQHCNAISSSTVDLLVEQLWRCDILS
Sbjct: 601 ADLIKLGETLVGLNIQHCNAISSSTVDLLVEQLWRCDILS 640

BLAST of PI0027335 vs. NCBI nr
Match: TYK31434.1 (EIN3-binding F-box protein 1 [Cucumis melo var. makuwa])

HSP 1 Score: 1176.4 bits (3042), Expect = 0.0e+00
Identity = 593/632 (93.83%), Postives = 606/632 (95.89%), Query Frame = 0

Query: 9   GSEDFCPGGSIYPNPKDSSLFLSLPHHVDVYFPPRKRSRITAPFVFGGEEVESKANVSIE 68
           GSEDFC GGSIYPNPKDSSLFLSLPHHVDVYFPPRKRSRITAPFVFGGEEVESKANVSIE
Sbjct: 15  GSEDFCTGGSIYPNPKDSSLFLSLPHHVDVYFPPRKRSRITAPFVFGGEEVESKANVSIE 74

Query: 69  ILPDECLFEIFRRLSGGKERSACATVSKRWLMLLSNIYSHELKSEDEVASKEVEDIEIES 128
           ILPDECLFEIFRRLSGGKERSACATVSKRWLMLLSNI SHELKSED VASKEVEDIEIES
Sbjct: 75  ILPDECLFEIFRRLSGGKERSACATVSKRWLMLLSNISSHELKSEDVVASKEVEDIEIES 134

Query: 129 DGYLSRSLEGKQATDLRLAAISVGTASRGGLGKLVIRGNNHVSKVTDLGLKAIARGCQSL 188
           DGYLSRSLEGK+ATDLRLAAISVGTA RGGLGKLVIRGNNHVSKVTDLGLKAIARGCQSL
Sbjct: 135 DGYLSRSLEGKKATDLRLAAISVGTAGRGGLGKLVIRGNNHVSKVTDLGLKAIARGCQSL 194

Query: 189 RALSLWNLSSIRDEGLCEIAKASHQLEKLDLCRCPAVSDKALVEIARNCPKLTDITIESC 248
           RALSLWNLSSIRD GLCEIAKASHQLEKLDLCRCPAVSDKA+VEIARNCPKLTDITIESC
Sbjct: 195 RALSLWNLSSIRDGGLCEIAKASHQLEKLDLCRCPAVSDKAVVEIARNCPKLTDITIESC 254

Query: 249 AKIGNESLRAIGQFCPKLKSIVIKDCPLVGDQGIASLLSLNTCALNKVKLQALNVSDVSL 308
           A+IGNESLRAIGQFC KLKSIVIKDCPLVGDQGIASLLSLNTCALNKVKLQALNVSDVSL
Sbjct: 255 AQIGNESLRAIGQFCTKLKSIVIKDCPLVGDQGIASLLSLNTCALNKVKLQALNVSDVSL 314

Query: 309 AVIGHYGKAVTDLVLTDLKNVSEKGFWVMGNGHGLQKLKSFTISSCNGVTDMGLESVGKG 368
           AVIGHYGKAVTDLVLTDLKNVSEKGFWVMGNGHGLQKLKSFTISSCNGVTDMGLESVGKG
Sbjct: 315 AVIGHYGKAVTDLVLTDLKNVSEKGFWVMGNGHGLQKLKSFTISSCNGVTDMGLESVGKG 374

Query: 369 SPNLKHFCLR--------------KSARSLECLQLEECHRITQFGFFGVVLNCSATLKAL 428
           SPNLKHFCLR              K+ARSLECLQLEECHRITQFGFFGVVLNCSA+LKAL
Sbjct: 375 SPNLKHFCLRKCSFLSDNGLVSFAKAARSLECLQLEECHRITQFGFFGVVLNCSASLKAL 434

Query: 429 SLISCLGIKDINSEFPIPASSGSLRSLTIRNCHGFGNRNLALLGKLCPQLQNVDFSGLVG 488
           SLISCLGIKDINSE PIPASSGSLRSLTIRNCHGFGNRNLALLGKLCPQLQNVDFSGLVG
Sbjct: 435 SLISCLGIKDINSELPIPASSGSLRSLTIRNCHGFGNRNLALLGKLCPQLQNVDFSGLVG 494

Query: 489 IDDCGFLAWLQNCQSGLVKINVNGCVNLTDKVVSSIIEHHGWSLKMLNLDGCKKITDASM 548
           IDDCGFLAWLQNCQSGLVKIN++GCVNLTD+V+SSIIEHHGW+LK+LNLD CKKITDASM
Sbjct: 495 IDDCGFLAWLQNCQSGLVKINLSGCVNLTDEVISSIIEHHGWTLKVLNLDSCKKITDASM 554

Query: 549 TSIANNCPLLSDLDVSKCSITDSGIAALAHAKRLNLQIFSISGCSFVSDKSLADLLNLGE 608
           TSIANNCPLLSDLDVSKCSITDSGIA LA AK+ NLQIFSISGCSFVSDKSLADL+ LGE
Sbjct: 555 TSIANNCPLLSDLDVSKCSITDSGIATLACAKQPNLQIFSISGCSFVSDKSLADLIKLGE 614

Query: 609 TLVGLNIQHCNAISSSTVDLLVEQLWRCDILS 627
           TLVGLNIQHCNAISSSTVDLLVEQLWRCDILS
Sbjct: 615 TLVGLNIQHCNAISSSTVDLLVEQLWRCDILS 646

BLAST of PI0027335 vs. NCBI nr
Match: XP_038897563.1 (EIN3-binding F-box protein 1 [Benincasa hispida])

HSP 1 Score: 1160.2 bits (3000), Expect = 0.0e+00
Identity = 582/640 (90.94%), Postives = 608/640 (95.00%), Query Frame = 0

Query: 1   MSKLFAFSGSEDFCPGGSIYPNPKDSSLFLSLPHHVDVYFPPRKRSRITAPFVFGGEEVE 60
           MSKLFA+SGSEDFCPGGSIYPNPKDSSLFLSLP+HVDVYFPPRK+SRI APFVFGG  VE
Sbjct: 1   MSKLFAYSGSEDFCPGGSIYPNPKDSSLFLSLPYHVDVYFPPRKKSRIIAPFVFGG-GVE 60

Query: 61  SKANVSIEILPDECLFEIFRRLSGGKERSACATVSKRWLMLLSNIYSHELKSEDEVASKE 120
           SKANVSIEILPDECLFEIFRRLSGGKERSACA+VSKRWLMLLSNI SHELKS+DEV  KE
Sbjct: 61  SKANVSIEILPDECLFEIFRRLSGGKERSACASVSKRWLMLLSNISSHELKSKDEVTLKE 120

Query: 121 VEDIEIESDGYLSRSLEGKQATDLRLAAISVGTASRGGLGKLVIRGNNHVSKVTDLGLKA 180
           VEDIEIESDGYLSRSLEGK+ATDLRLAAISVGTASRGGLGKLVIRG NHVSKVTDLGLK 
Sbjct: 121 VEDIEIESDGYLSRSLEGKKATDLRLAAISVGTASRGGLGKLVIRGTNHVSKVTDLGLKT 180

Query: 181 IARGCQSLRALSLWNLSSIRDEGLCEIAKASHQLEKLDLCRCPAVSDKALVEIARNCPKL 240
           IARGCQSLRALSLWNLSSI DEGLCEIAKASH LEKLDLCRCPAVSDKA+VEIAR+CPKL
Sbjct: 181 IARGCQSLRALSLWNLSSIGDEGLCEIAKASHHLEKLDLCRCPAVSDKAVVEIARSCPKL 240

Query: 241 TDITIESCAKIGNESLRAIGQFCPKLKSIVIKDCPLVGDQGIASLLSLNTCALNKVKLQA 300
           TDITIESCAKIGN SLRAIGQFCPKLKSIVIKDCPLVGDQGIASLLSLNTCALNKVKLQA
Sbjct: 241 TDITIESCAKIGNGSLRAIGQFCPKLKSIVIKDCPLVGDQGIASLLSLNTCALNKVKLQA 300

Query: 301 LNVSDVSLAVIGHYGKAVTDLVLTDLKNVSEKGFWVMGNGHGLQKLKSFTISSCNGVTDM 360
           L VSDVSLAVIGHYGKAVTDLVLTDLKNVSEKGFWVMGNGHGLQKLKSFT+SSCNGVTDM
Sbjct: 301 LKVSDVSLAVIGHYGKAVTDLVLTDLKNVSEKGFWVMGNGHGLQKLKSFTVSSCNGVTDM 360

Query: 361 GLESVGKGSPNLKHFCLR--------------KSARSLECLQLEECHRITQFGFFGVVLN 420
           GLESVGKGSPNLKHFCLR              K+ARSLECLQLEECHRITQFGFFGV+LN
Sbjct: 361 GLESVGKGSPNLKHFCLRKCSFLSDNGLVSFAKAARSLECLQLEECHRITQFGFFGVLLN 420

Query: 421 CSATLKALSLISCLGIKDINSEFPIPASSGSLRSLTIRNCHGFGNRNLALLGKLCPQLQN 480
           CS +LKALSLISCLGIKD+NSE  +PASSGSLRSLTIRNCHGFGNRNLALLGKLCPQLQN
Sbjct: 421 CSTSLKALSLISCLGIKDMNSELAMPASSGSLRSLTIRNCHGFGNRNLALLGKLCPQLQN 480

Query: 481 VDFSGLVGIDDCGFLAWLQNCQSGLVKINVNGCVNLTDKVVSSIIEHHGWSLKMLNLDGC 540
           VDFSGLVGIDDCG LAWLQNCQSGL+KINV+GCVNLTDKVVSS+ EHHGW+LKMLNLDGC
Sbjct: 481 VDFSGLVGIDDCGLLAWLQNCQSGLMKINVSGCVNLTDKVVSSMTEHHGWTLKMLNLDGC 540

Query: 541 KKITDASMTSIANNCPLLSDLDVSKCSITDSGIAALAHAKRLNLQIFSISGCSFVSDKSL 600
           +KITDAS+TSIANNCP LS+LDVSKCSITDSG+AALAHAK+L+LQIFSISGCSFVSDKSL
Sbjct: 541 RKITDASLTSIANNCPSLSNLDVSKCSITDSGVAALAHAKQLSLQIFSISGCSFVSDKSL 600

Query: 601 ADLLNLGETLVGLNIQHCNAISSSTVDLLVEQLWRCDILS 627
           ADL+NLG+TLVGLNIQHCNAISSSTVDLLVEQLWRCDILS
Sbjct: 601 ADLINLGDTLVGLNIQHCNAISSSTVDLLVEQLWRCDILS 639

BLAST of PI0027335 vs. NCBI nr
Match: XP_022133120.1 (EIN3-binding F-box protein 1 [Momordica charantia])

HSP 1 Score: 1136.3 bits (2938), Expect = 0.0e+00
Identity = 564/641 (87.99%), Postives = 603/641 (94.07%), Query Frame = 0

Query: 1   MSKLFAFSGSEDFCPGGSIYPNPKDSSLFLSLPHHVDVYFPPRKRSRITAPFVFGGEEVE 60
           MSKLFA+SGSEDFCPGGSIYP+ KDSSLFLSLPHHVDVYFPPRKRSRITAPFVFGGE VE
Sbjct: 1   MSKLFAYSGSEDFCPGGSIYPSSKDSSLFLSLPHHVDVYFPPRKRSRITAPFVFGGEGVE 60

Query: 61  SKANVSIEILPDECLFEIFRRLSGGKERSACATVSKRWLMLLSNIYSHELKSEDEVASKE 120
           SKANVSIE+LPDECLFEIFRRL GGKERSACA VSK+WLMLLSNI  HELKS DEVASKE
Sbjct: 61  SKANVSIEVLPDECLFEIFRRLPGGKERSACAGVSKKWLMLLSNISCHELKSGDEVASKE 120

Query: 121 VEDIEIESDGYLSRSLEGKQATDLRLAAISVGTASRGGLGKLVIRGNNHVSKVTDLGLKA 180
           VED+EIESDGYLSRSLEGK+ATDLRLAAISVGTASRGGLGKLVIRGNNH SKVTDLGL+A
Sbjct: 121 VEDMEIESDGYLSRSLEGKKATDLRLAAISVGTASRGGLGKLVIRGNNHASKVTDLGLRA 180

Query: 181 IARGCQSLRALSLWNLSSIRDEGLCEIAKASHQLEKLDLCRCPAVSDKALVEIARNCPKL 240
           +ARGCQSLRALSLWNLSSI DEGLCEIA  SHQLEKLDLCRCPA+SDKA++ IARNCPKL
Sbjct: 181 VARGCQSLRALSLWNLSSIGDEGLCEIASVSHQLEKLDLCRCPAISDKAVIAIARNCPKL 240

Query: 241 TDITIESCAKIGNESLRAIGQFCPKLKSIVIKDCPLVGDQGIASLLSLNTCALNKVKLQA 300
           TDITIESCAKIGNESLRAIGQ CPKLKSIVIKDCPLVGDQGIASLLSLNTCALNKVKLQA
Sbjct: 241 TDITIESCAKIGNESLRAIGQCCPKLKSIVIKDCPLVGDQGIASLLSLNTCALNKVKLQA 300

Query: 301 LNVSDVSLAVIGHYGKAVTDLVLTDLKNVSEKGFWVMGNGHGLQKLKSFTISSCNGVTDM 360
           LN+SDVSLAVIGHYGK+VTDLVL+DLKNVSEKGFWVMGNGHGLQKLKSFTISSCNGVTDM
Sbjct: 301 LNISDVSLAVIGHYGKSVTDLVLSDLKNVSEKGFWVMGNGHGLQKLKSFTISSCNGVTDM 360

Query: 361 GLESVGKGSPNLKHFCLR--------------KSARSLECLQLEECHRITQFGFFGVVLN 420
           GLESVGKGSPNLKHFCLR              K+ARSLE LQLEECHRITQFGFFGV+LN
Sbjct: 361 GLESVGKGSPNLKHFCLRKCSFLSDSGLVSFAKAARSLENLQLEECHRITQFGFFGVLLN 420

Query: 421 CSATLKALSLISCLGIKDINSEFPIPASSG-SLRSLTIRNCHGFGNRNLALLGKLCPQLQ 480
           CS +LKALSL+SCLGIKD++SE PIP+SSG SLRSLTIRNCHGFGN+NLA LGKLCPQLQ
Sbjct: 421 CSTSLKALSLVSCLGIKDMSSESPIPSSSGSSLRSLTIRNCHGFGNKNLASLGKLCPQLQ 480

Query: 481 NVDFSGLVGIDDCGFLAWLQNCQSGLVKINVNGCVNLTDKVVSSIIEHHGWSLKMLNLDG 540
           +VDFSGLVGIDDCGFLAWLQNCQSGLVK+N++GCVNLTDK VSSIIEHHGW+L++LNLDG
Sbjct: 481 HVDFSGLVGIDDCGFLAWLQNCQSGLVKLNISGCVNLTDKAVSSIIEHHGWTLELLNLDG 540

Query: 541 CKKITDASMTSIANNCPLLSDLDVSKCSITDSGIAALAHAKRLNLQIFSISGCSFVSDKS 600
           C+KITDAS+ SIANNCPLLSDLD+SKCSI DSGIAALAHAK+L+LQIFS+SGCS VSDKS
Sbjct: 541 CRKITDASLVSIANNCPLLSDLDISKCSIMDSGIAALAHAKQLSLQIFSVSGCSLVSDKS 600

Query: 601 LADLLNLGETLVGLNIQHCNAISSSTVDLLVEQLWRCDILS 627
           L +L+ LGETL+GLNIQHCN+ISSSTVDLLVEQLWRCDILS
Sbjct: 601 LENLIKLGETLMGLNIQHCNSISSSTVDLLVEQLWRCDILS 641

BLAST of PI0027335 vs. TAIR 10
Match: AT2G25490.1 (EIN3-binding F box protein 1 )

HSP 1 Score: 757.7 bits (1955), Expect = 7.5e-219
Identity = 387/640 (60.47%), Postives = 491/640 (76.72%), Query Frame = 0

Query: 1   MSKLFAFSGSEDFCPGGSIYPNPKDSSLFLSLPHHVDVYFPPRKRSRITAPFVFGGEEVE 60
           MS++F+F+G  DF   G+IYPNPKD+SL LSL    DVYFPP KRSR+ AP +F   E  
Sbjct: 1   MSQIFSFAGENDFYRRGAIYPNPKDASLLLSLGSFADVYFPPSKRSRVVAPTIFSAFE-- 60

Query: 61  SKANVSIEILPDECLFEIFRRLSGGKERSACATVSKRWLMLLSNIYSHELKSEDEVASKE 120
            K  VSI++LPDECLFEIFRRLSG +ERSACA VSK+WL L+S+I   E+    +V SK 
Sbjct: 61  -KKPVSIDVLPDECLFEIFRRLSGPQERSACAFVSKQWLTLVSSIRQKEI----DVPSKI 120

Query: 121 VEDIEIESDGYLSRSLEGKQATDLRLAAISVGTASRGGLGKLVIRGNNHVSKVTDLGLKA 180
            ED + + +G LSRSL+GK+ATD+RLAAI+VGTA RGGLGKL IRG+N  +KV+DLGL++
Sbjct: 121 TEDGD-DCEGCLSRSLDGKKATDVRLAAIAVGTAGRGGLGKLSIRGSNS-AKVSDLGLRS 180

Query: 181 IARGCQSLRALSLWNLSSIRDEGLCEIAKASHQLEKLDLCRCPAVSDKALVEIARNCPKL 240
           I R C SL +LSLWN+S+I D GL EIA+   QLEKL+L RC  ++DK LV IA++CP L
Sbjct: 181 IGRSCPSLGSLSLWNVSTITDNGLLEIAEGCAQLEKLELNRCSTITDKGLVAIAKSCPNL 240

Query: 241 TDITIESCAKIGNESLRAIGQFCPKLKSIVIKDCPLVGDQGIASLLSLNTCALNKVKLQA 300
           T++T+E+C++IG+E L AI + C KLKS+ IK+CPLV DQGIASLLS  TC+L K+KLQ 
Sbjct: 241 TELTLEACSRIGDEGLLAIARSCSKLKSVSIKNCPLVRDQGIASLLSNTTCSLAKLKLQM 300

Query: 301 LNVSDVSLAVIGHYGKAVTDLVLTDLKNVSEKGFWVMGNGHGLQKLKSFTISSCNGVTDM 360
           LNV+DVSLAV+GHYG ++TDLVL  L +VSEKGFWVMGNG GLQKL S TI++C GVTDM
Sbjct: 301 LNVTDVSLAVVGHYGLSITDLVLAGLSHVSEKGFWVMGNGVGLQKLNSLTITACQGVTDM 360

Query: 361 GLESVGKGSPNLKHFCLRKS--------------ARSLECLQLEECHRITQFGFFGVVLN 420
           GLESVGKG PN+K   + KS              + SLE LQLEECHR+TQFGFFG +LN
Sbjct: 361 GLESVGKGCPNMKKAIISKSPLLSDNGLVSFAKASLSLESLQLEECHRVTQFGFFGSLLN 420

Query: 421 CSATLKALSLISCLGIKDINSEFPIPASSGSLRSLTIRNCHGFGNRNLALLGKLCPQLQN 480
           C   LKA SL++CL I+D+ +  P  +   +LRSL+IRNC GFG+ NLA +GKLCPQL++
Sbjct: 421 CGEKLKAFSLVNCLSIRDLTTGLPASSHCSALRSLSIRNCPGFGDANLAAIGKLCPQLED 480

Query: 481 VDFSGLVGIDDCGFLAWLQNCQSGLVKINVNGCVNLTDKVVSSIIEHHGWSLKMLNLDGC 540
           +D  GL GI + GFL  +   QS LVKIN +GC NLTD+V+S+I   +GW+L++LN+DGC
Sbjct: 481 IDLCGLKGITESGFLHLI---QSSLVKINFSGCSNLTDRVISAITARNGWTLEVLNIDGC 540

Query: 541 KKITDASMTSIANNCPLLSDLDVSKCSITDSGIAALAHAKRLNLQIFSISGCSFVSDKSL 600
             ITDAS+ SIA NC +LSDLD+SKC+I+DSGI ALA + +L LQI S++GCS V+DKSL
Sbjct: 541 SNITDASLVSIAANCQILSDLDISKCAISDSGIQALASSDKLKLQILSVAGCSMVTDKSL 600

Query: 601 ADLLNLGETLVGLNIQHCNAISSSTVDLLVEQLWRCDILS 627
             ++ LG TL+GLN+Q C +IS+STVD LVE+L++CDILS
Sbjct: 601 PAIVGLGSTLLGLNLQQCRSISNSTVDFLVERLYKCDILS 628

BLAST of PI0027335 vs. TAIR 10
Match: AT5G25350.1 (EIN3-binding F box protein 2 )

HSP 1 Score: 669.5 bits (1726), Expect = 2.7e-192
Identity = 359/641 (56.01%), Postives = 449/641 (70.05%), Query Frame = 0

Query: 1   MSKLFAFSGSEDFCPGGSIYPNPKDSSLFLSLPHHVDVYFPPRKRSRITAPFVFGGEEVE 60
           MS +F FSG ED   GGS+Y +P       S P    VY+P RKR R+ A   + G E  
Sbjct: 1   MSGIFRFSGDEDCLLGGSMYLSPG------SCP---GVYYPARKRLRVAATSFYSGFE-- 60

Query: 61  SKANVSIEILPDECLFEIFRRLSGGKERSACATVSKRWLMLLSNIYSHELKSEDEVASKE 120
            +   SI++LP+ECLFEI RRL  G+ERSACA VSK WL LLS+I      S  EV    
Sbjct: 61  -EKQTSIDVLPEECLFEILRRLPSGQERSACACVSKHWLNLLSSI------SRSEVNESS 120

Query: 121 VEDIEIESDGYLSRSLEGKQATDLRLAAISVGTASRGGLGKLVIRGNNHVSKVTDLGLKA 180
           V+D+E E +G+LSRSLEGK+ATDLRLAAI+VGT+SRGGLGKL IRG+   SKVTD+GL A
Sbjct: 121 VQDVE-EGEGFLSRSLEGKKATDLRLAAIAVGTSSRGGLGKLQIRGSGFESKVTDVGLGA 180

Query: 181 IARGCQSLRALSLWNLSSIRDEGLCEIAKASHQLEKLDLCRCPAVSDKALVEIARNCPKL 240
           +A GC SLR +SLWNL ++ D GL EIA++   +EKLDL RCP ++D  LV IA NC  L
Sbjct: 181 VAHGCPSLRIVSLWNLPAVSDLGLSEIARSCPMIEKLDLSRCPGITDSGLVAIAENCVNL 240

Query: 241 TDITIESCAKIGNESLRAIGQFCPKLKSIVIKDCPLVGDQGIASLLSLNTCALNKVKLQA 300
           +D+TI+SC+ +GNE LRAI + C  L+SI I+ CP +GDQG+A LL+     L KVKLQ 
Sbjct: 241 SDLTIDSCSGVGNEGLRAIARRCVNLRSISIRSCPRIGDQGVAFLLAQAGSYLTKVKLQM 300

Query: 301 LNVSDVSLAVIGHYGKAVTDLVLTDLKNVSEKGFWVMGNGHGLQKLKSFTISSCNGVTDM 360
           LNVS +SLAVIGHYG AVTDLVL  L+ V+EKGFWVMGN  GL+KLKS ++ SC G+TD+
Sbjct: 301 LNVSGLSLAVIGHYGAAVTDLVLHGLQGVNEKGFWVMGNAKGLKKLKSLSVMSCRGMTDV 360

Query: 361 GLESVGKGSPNLKH--------------FCLRKSARSLECLQLEECHRITQFGFFGVVLN 420
           GLE+VG G P+LKH                L KSA SLE L+LEECHRI QFG  G ++N
Sbjct: 361 GLEAVGNGCPDLKHVSLNKCLLVSGKGLVALAKSALSLESLKLEECHRINQFGLMGFLMN 420

Query: 421 CSATLKALSLISCLGIKDINSEFPIPA-SSGSLRSLTIRNCHGFGNRNLALLGKLCPQLQ 480
           C + LKA SL +CLGI D NSE  +P+ S  SLRSL+IR C GFG+ +LA LGK C QLQ
Sbjct: 421 CGSKLKAFSLANCLGISDFNSESSLPSPSCSSLRSLSIRCCPGFGDASLAFLGKFCHQLQ 480

Query: 481 NVDFSGLVGIDDCGFLAWLQNCQSGLVKINVNGCVNLTDKVVSSIIEHHGWSLKMLNLDG 540
           +V+  GL G+ D G    LQ+   GLVK+N++ C+N++D  VS+I   HG +L+ LNLDG
Sbjct: 481 DVELCGLNGVTDAGVRELLQSNNVGLVKVNLSECINVSDNTVSAISVCHGRTLESLNLDG 540

Query: 541 CKKITDASMTSIANNCPLLSDLDVSKCSITDSGIAALAHA-KRLNLQIFSISGCSFVSDK 600
           CK IT+AS+ ++A NC  ++DLD+S   ++D GI ALA +   LNLQ+ SI GCS ++DK
Sbjct: 541 CKNITNASLVAVAKNCYSVNDLDISNTLVSDHGIKALASSPNHLNLQVLSIGGCSSITDK 600

Query: 601 SLADLLNLGETLVGLNIQHCNAISSSTVDLLVEQLWRCDIL 626
           S A +  LG TL+GLNIQ C  ISSSTVD L+E LWRCDIL
Sbjct: 601 SKACIQKLGRTLLGLNIQRCGRISSSTVDTLLENLWRCDIL 622

BLAST of PI0027335 vs. TAIR 10
Match: AT4G15475.1 (F-box/RNI-like superfamily protein )

HSP 1 Score: 187.6 bits (475), Expect = 3.1e-47
Identity = 166/587 (28.28%), Postives = 278/587 (47.36%), Query Frame = 0

Query: 70  LPDECLFEIFRRLSGGKERSACATVSKRWLML----LSNIYSHELKSEDEVAS----KEV 129
           LP+E + EIFRRL     R AC+ V KRWL L     + +      S D+  S    + +
Sbjct: 11  LPEELILEIFRRLESKPNRDACSLVCKRWLSLERFSRTTLRIGASFSPDDFISLLSRRFL 70

Query: 130 EDIEIESDGYLSRSLEGKQATDLRLAAISVGTASRGGLGKLVIR-----GNNHVSKVTDL 189
               I  D  +S SL     +  R       + S     KL  +      N   S +TD 
Sbjct: 71  YITSIHVDERISVSLPSLSPSPKRKRGRDSSSPSSSKRKKLTDKTHSGAENVESSSLTDT 130

Query: 190 GLKAIARGCQSLRALSL-W--NLSS----------------------IRDEGLCEIAKAS 249
           GL A+A G   +  LSL W  N+SS                      + D+GL  + K  
Sbjct: 131 GLTALANGFPRIENLSLIWCPNVSSVGLCSLAQKCTSLKSLDLQGCYVGDQGLAAVGKFC 190

Query: 250 HQLEKLDLCRCPAVSDKALVEIARNCPK-LTDITIESCAKIGNESLRAIGQFCPKLKSIV 309
            QLE+L+L  C  ++D  ++++   C K L  I + + AKI + SL A+G  C KL  ++
Sbjct: 191 KQLEELNLRFCEGLTDVGVIDLVVGCSKSLKSIGVAASAKITDLSLEAVGSHC-KLLEVL 250

Query: 310 IKDCPLVGDQGIASLLSLNTCALNKVKLQALNVSDVSLAVIGHYGKAVTDLVLTDLKNVS 369
             D   + D+G+ + ++     L  +KLQ ++V+DV+ A +G    ++  L L   ++ +
Sbjct: 251 YLDSEYIHDKGLIA-VAQGCHRLKNLKLQCVSVTDVAFAAVGELCTSLERLALYSFQHFT 310

Query: 370 EKGFWVMGNGHGLQKLKSFTISSCNGVTDMGLESVGKGSPNLKHFCLRKSARSLECLQLE 429
           +KG   +G G   +KLK  T+S C  V+  GLE++  G             + LE +++ 
Sbjct: 311 DKGMRAIGKGS--KKLKDLTLSDCYFVSCKGLEAIAHG------------CKELERVEIN 370

Query: 430 ECHRITQFGFFGVVLNCSATLKALSLISCLGIKDINSEFPIPASSGSLRSLTIRNCHGFG 489
            CH I   G   +  +C   LK L+L+ C  I + ++   I     SL  L + +C G G
Sbjct: 371 GCHNIGTRGIEAIGKSC-PRLKELALLYCQRIGN-SALQEIGKGCKSLEILHLVDCSGIG 430

Query: 490 NRNLALLGKLCPQLQNVDFSGLVGIDDCGFLAWLQNCQSGLVKINVNGCVNLTDKVVSSI 549
           +  +  + K C  L+ +       I + G ++  ++C+S L ++++  C  + +K + +I
Sbjct: 431 DIAMCSIAKGCRNLKKLHIRRCYEIGNKGIISIGKHCKS-LTELSLRFCDKVGNKALIAI 490

Query: 550 IEHHGWSLKMLNLDGCKKITDASMTSIANNCPLLSDLDVSKC-SITDSGIAALAHAKRLN 609
               G SL+ LN+ GC +I+DA +T+IA  CP L+ LD+S   +I D  +A L     + 
Sbjct: 491 --GKGCSLQQLNVSGCNQISDAGITAIARGCPQLTHLDISVLQNIGDMPLAELGEGCPM- 550

Query: 610 LQIFSISGCSFVSDKSLADLLNLGETLVGLNIQHCNAISSSTVDLLV 617
           L+   +S C  ++D  L  L+   + L   ++ +C  I+S+ V  +V
Sbjct: 551 LKDLVLSHCHHITDNGLNHLVQKCKLLETCHMVYCPGITSAGVATVV 575

BLAST of PI0027335 vs. TAIR 10
Match: AT5G01720.1 (RNI-like superfamily protein )

HSP 1 Score: 135.6 bits (340), Expect = 1.4e-31
Identity = 135/475 (28.42%), Postives = 219/475 (46.11%), Query Frame = 0

Query: 141 ATDLRLAAISVGTASRGGLGKLVIRGNNHVSKVTDLGLKAIARGCQSLRALSLWNLSSIR 200
           AT++R A  +V   +R  L +L +        +TD+G+  IA GC+ L  +SL     + 
Sbjct: 135 ATEMRDADAAVVAEAR-SLERLKL---GRCKMLTDMGIGCIAVGCKKLNTVSLKWCVGVG 194

Query: 201 DEGLCEIAKASHQLEKLDLCRCPAVSDKALVEIARNCPKLTDITIESCAKIGNESLRAIG 260
           D G+  +A     +  LDL   P ++ K L +I +    L ++ +E C  + ++SL+++ 
Sbjct: 195 DLGVGLLAVKCKDIRTLDLSYLP-ITGKCLHDILK-LQHLEELLLEGCFGVDDDSLKSLR 254

Query: 261 QFCPKLKSIVIKDCPLVGDQGIASLLSLNTCALNKVKLQALNVSDVSLAVIGHYG---KA 320
             C  LK +    C  +  +G+ SLLS          LQ L++S  S  +   +    K 
Sbjct: 255 HDCKSLKKLDASSCQNLTHRGLTSLLS------GAGYLQRLDLSHCSSVISLDFASSLKK 314

Query: 321 VTDLVLTDLKNVSEKGFWVMGNGHGLQKLKSFTISSCNGVTDMGLESVGKGSPNLKHF-- 380
           V+ L    L   S     +   G     LK  ++S C  VTD GL S+     +L+    
Sbjct: 315 VSALQSIRLDGCSVTPDGLKAIGTLCNSLKEVSLSKCVSVTDEGLSSLVMKLKDLRKLDI 374

Query: 381 -CLRKSAR-----------SLECLQLEECHRITQFGFFGVVLNCSATLKALSL----ISC 440
            C RK +R            L  L++E C  +++  F+ +   C   L+ L L    I  
Sbjct: 375 TCCRKLSRVSITQIANSCPLLVSLKMESCSLVSREAFWLIGQKC-RLLEELDLTDNEIDD 434

Query: 441 LGIKDINSEFPIPASSGSLRSLTIRNCHGFGNRNLALLGKLCPQLQNVDFSGLVGIDDCG 500
            G+K I+S         SL SL +  C    ++ L+ +G  C  L+ +D    VGI D G
Sbjct: 435 EGLKSISSCL-------SLSSLKLGICLNITDKGLSYIGMGCSNLRELDLYRSVGITDVG 494

Query: 501 FLAWLQNCQSGLVKINVNGCVNLTDKVVSSIIEHHGWSLKMLNLDGCKKITDASMTSIAN 560
                Q C   L  IN++ C ++TDK + S+ +     L+     GC  IT   + +IA 
Sbjct: 495 ISTIAQGC-IHLETINISYCQDITDKSLVSLSKCS--LLQTFESRGCPNITSQGLAAIAV 554

Query: 561 NCPLLSDLDVSKC-SITDSGIAALAHAKRLNLQIFSISGCSFVSDKSLADLLNLG 594
            C  L+ +D+ KC SI D+G+ ALAH  + NL+  ++S  + V++  L  L N+G
Sbjct: 555 RCKRLAKVDLKKCPSINDAGLLALAHFSQ-NLKQINVSDTA-VTEVGLLSLANIG 584

BLAST of PI0027335 vs. TAIR 10
Match: AT5G23340.1 (RNI-like superfamily protein )

HSP 1 Score: 116.7 bits (291), Expect = 6.7e-26
Identity = 100/413 (24.21%), Postives = 179/413 (43.34%), Query Frame = 0

Query: 68  EILPDECLFEIFRRLSGGKERSACATVSKRWLMLLS--------NIYSHELKSEDEVASK 127
           E L D+ L  +  RL   K++     V KRWL L S            H L+    +AS+
Sbjct: 8   EALTDDELRWVLSRLDSDKDKEVFGLVCKRWLNLQSTDRKKLAARAGPHMLR---RLASR 67

Query: 128 EVEDIEIESDGYLSRSLEGKQATDLRLAAISVGTASRGGLGKLVIRGNNHVSKVTDLGLK 187
             + +E++    +SRS      TD  LA IS       G   L +   ++   +TD GL 
Sbjct: 68  FTQIVELDLSQSISRSFY-PGVTDSDLAVIS------EGFKFLRVLNLHNCKGITDTGLA 127

Query: 188 AIARGCQSLRALSLWNLSSIRDEGLCEIAKASHQLEKLDLCRCPAVSDKALVEIARNCPK 247
           +I R    L+ L +     + D+GL  +A+  H L  L L  C  ++D++L  ++  C  
Sbjct: 128 SIGRCLSLLQFLDVSYCRKLSDKGLSAVAEGCHDLRALHLAGCRFITDESLKSLSERCRD 187

Query: 248 LTDITIESCAKIGNESLRAIGQFCPKLKSIVIKDCPLVGDQGIASLLSLNTCALNKVK-L 307
           L  + ++ C  I +  L  + + C K+KS+ I  C  VGD G++S+      +L  +K L
Sbjct: 188 LEALGLQGCTNITDSGLADLVKGCRKIKSLDINKCSNVGDAGVSSVAKACASSLKTLKLL 247

Query: 308 QALNVSDVSLAVIGHYGKAVTDLVLTDLKNVSEKGFWVMGNGHGLQKLKSFTISSCNGVT 367
               V + S++ +  + K +  L++   +++S++   ++ +      LK+  +  C  ++
Sbjct: 248 DCYKVGNESISSLAQFCKNLETLIIGGCRDISDESIMLLADS-CKDSLKNLRMDWCLNIS 307

Query: 368 DMGLESVGKGSPNLKHFCLRKSARSLECLQLEECHRITQFGFFGVVLNCSATLKALSLIS 427
           D  L             C+ K  ++LE L +  C  +T   F  +  +    LK L + +
Sbjct: 308 DSSLS------------CILKQCKNLEALDIGCCEEVTDTAFRDLGSDDVLGLKVLKVSN 367

Query: 428 CLGIKDINSEFPIPASSGSLRSLTIRNCHGFGNRNLALLGKLCPQLQNVDFSG 472
           C  I  +     +     SL  + +R+         +  G   P+   V+FSG
Sbjct: 368 CTKI-TVTGIGKLLDKCSSLEYIDVRSLPHVTEVRCSEAGLEFPKCCKVNFSG 396

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SKK01.1e-21760.47EIN3-binding F-box protein 1 OS=Arabidopsis thaliana OX=3702 GN=EBF1 PE=1 SV=1[more]
Q708Y03.8e-19156.01EIN3-binding F-box protein 2 OS=Arabidopsis thaliana OX=3702 GN=EBF2 PE=1 SV=1[more]
Q9C5D24.4e-4628.28F-box/LRR-repeat protein 4 OS=Arabidopsis thaliana OX=3702 GN=FBL4 PE=2 SV=1[more]
Q8RWU52.0e-3028.42F-box/LRR-repeat protein 3 OS=Arabidopsis thaliana OX=3702 GN=FBL3 PE=2 SV=1[more]
A6H7793.7e-2128.70F-box/LRR-repeat protein 2 OS=Bos taurus OX=9913 GN=FBXL2 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A059VBN50.0e+0094.22EIN3-binding-Fbox protein 1 OS=Cucumis sativus OX=3659 GN=Csa_3G878200 PE=2 SV=1[more]
A0A5A7TB220.0e+0093.91EIN3-binding F-box protein 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_sca... [more]
A0A1S4E5P70.0e+0093.91EIN3-binding F-box protein 1 OS=Cucumis melo OX=3656 GN=LOC103503821 PE=4 SV=1[more]
A0A5D3E6330.0e+0093.83EIN3-binding F-box protein 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca... [more]
A0A6J1BUE60.0e+0087.99EIN3-binding F-box protein 1 OS=Momordica charantia OX=3673 GN=LOC111005790 PE=4... [more]
Match NameE-valueIdentityDescription
NP_001281254.10.0e+0094.22EIN3-binding F-box protein 1-like [Cucumis sativus] >AHZ89385.1 EIN3-binding-Fbo... [more]
XP_016903542.10.0e+0093.91PREDICTED: EIN3-binding F-box protein 1 [Cucumis melo] >KAA0038811.1 EIN3-bindin... [more]
TYK31434.10.0e+0093.83EIN3-binding F-box protein 1 [Cucumis melo var. makuwa][more]
XP_038897563.10.0e+0090.94EIN3-binding F-box protein 1 [Benincasa hispida][more]
XP_022133120.10.0e+0087.99EIN3-binding F-box protein 1 [Momordica charantia][more]
Match NameE-valueIdentityDescription
AT2G25490.17.5e-21960.47EIN3-binding F box protein 1 [more]
AT5G25350.12.7e-19256.01EIN3-binding F box protein 2 [more]
AT4G15475.13.1e-4728.28F-box/RNI-like superfamily protein [more]
AT5G01720.11.4e-3128.42RNI-like superfamily protein [more]
AT5G23340.16.7e-2624.21RNI-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001810F-box domainSMARTSM00256fbox_2coord: 70..111
e-value: 7.0E-5
score: 32.2
IPR001810F-box domainPFAMPF12937F-box-likecoord: 69..102
e-value: 8.8E-6
score: 25.5
IPR006553Leucine-rich repeat, cysteine-containing subtypeSMARTSM00367LRR_CC_2coord: 461..486
e-value: 21.0
score: 12.0
coord: 488..513
e-value: 630.0
score: 0.5
coord: 541..565
e-value: 0.0049
score: 26.1
coord: 435..460
e-value: 65.0
score: 8.2
coord: 237..262
e-value: 0.035
score: 23.2
coord: 185..210
e-value: 0.67
score: 19.0
coord: 211..236
e-value: 2.2E-4
score: 30.6
coord: 515..540
e-value: 1.8
score: 17.6
coord: 343..368
e-value: 0.88
score: 18.6
coord: 567..592
e-value: 7.8
score: 15.4
coord: 381..406
e-value: 120.0
score: 6.1
coord: 263..288
e-value: 0.0013
score: 28.0
NoneNo IPR availableGENE3D1.20.1280.50coord: 60..108
e-value: 5.9E-6
score: 28.1
NoneNo IPR availablePANTHERPTHR13318UNCHARACTERIZEDcoord: 17..617
NoneNo IPR availablePANTHERPTHR13318:SF88EIN3-BINDING F-BOX PROTEIN 1coord: 17..617
NoneNo IPR availableSUPERFAMILY52047RNI-likecoord: 171..398
NoneNo IPR availableSUPERFAMILY52047RNI-likecoord: 382..612
IPR032675Leucine-rich repeat domain superfamilyGENE3D3.80.10.10Ribonuclease Inhibitorcoord: 134..249
e-value: 1.6E-19
score: 71.5
coord: 250..427
e-value: 6.6E-25
score: 89.3
IPR032675Leucine-rich repeat domain superfamilyGENE3D3.80.10.10Ribonuclease Inhibitorcoord: 429..626
e-value: 1.0E-31
score: 112.2
IPR001611Leucine-rich repeatPFAMPF13516LRR_6coord: 541..563
e-value: 0.047
score: 13.8
IPR036047F-box-like domain superfamilySUPERFAMILY81383F-box domaincoord: 61..104

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0027335.1PI0027335.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0031146 SCF-dependent proteasomal ubiquitin-dependent protein catabolic process
cellular_component GO:0019005 SCF ubiquitin ligase complex
molecular_function GO:0005515 protein binding