PI0027280 (gene) Melon (PI 482460) v1

Overview
NamePI0027280
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
DescriptionEukaryotic aspartyl protease family protein
Locationchr02: 21812909 .. 21814809 (+)
RNA-Seq ExpressionPI0027280
SyntenyPI0027280
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATGATTCTGTTAACTTCTCTCCATCATCTTTTACCTTCCTTAACCTTGGCATTTTATTTGTCAACAGCCGTAATCTCGTCAGCATCGATCACAACAAAGCCTTCACGTTTGGCAACCAAGCTGATCCACCGCAACTCGTATCTACATCCACTCTATGACCCAAATGAGACAGTTGAGGATCAATCGAAGAGAGAGCAAACAAGCTCAATCGAACGCTTTGCTTATCTCGAGTCGAAGATTAAAGAACTGAAGTCTGTTGGTAATGAAGCTCGATCAAGTCTCATTCCTTTCAATCGAGGTAGTGGGTTTCTTGTTAATTTGTCTATTGGTTCGCCACCCGTGACACAGCTCGTAGTGGTCGACACTGGTAGCTCCCTCCTGTGGGTGCAATGTTTGCCTTGTATCAACTGTTTTCAACAATCGAGCTCATGGTTCGATCCTTTGAAATCAGCAAGTTTCAAAACATTGGGTTGCGGGTTTCCTGGGTATAAATACATTAATGGTTACAAATGCAATGGGTTTAATCAAGCTGAGTACAAGTTGAGGTACCTTGGTGGGGATTCCTCACAAGGAATTCTTGCTAAGGAATCACTTCTCTTTGAGACACTTGATGAAGGTAGAGTTTTTCAATACAATGCTATTTCAACACAAATAAGTTAATAGATAAATTTGACATTATTAATTGATTTAGTAACATATTTTACCGTTTTGTATAATTATTATTTGTGATATCATATGATTTGTAATACACTATATGATACACATTTTAATTTAACAAAAAAGAATTAAGATTATAACATAGAAAAACTATATGAACTTTAATTTGTAATTACAACATTTAATCAAACTCACAATGGCTAAATTCAGTATTCGGTTGTTATAACAGGCAAGATAAAAAAGACGAACTTAACATTTGGGTGTGGTCATATGAACTTCAAAACCAACAATGACGATACCTACAATGGCGTATTTGGATTAGGAGCATATCCCCACATAACAATGGCCACTCAATTAGGCAACAAATTTTCCTATTGCATTGGCGATATCAACAATCCTCTCTACACTCACAATCATCTCGTCTTAGGACAAGGATCTTACATTGAAGGCGATTTCACTCCTCTTCAAATCCACTTTGGCCATTACTATGTCACTTTACAATCCATTAGTGTTGGCTCTAAGACCCTCAAAATCGACCCAAATGCCTTCAAAATCTCATCGGACGGCAGCGGCGGAGTTCTGATCGACTCTGGAATGACCTACACTAAGCTTGCGAATGGTGGGTTTGAATTACTTTATGATGAGATTGTTGATTTGATGAAGGGTTTGCTGGAACGAATCCCAACTCAGAGGAAATTTGAAGGGTTGTGTTTTAAAGGTGTTGTGAGTCGAGATCTTGTTGGGTTTCCGGCTGTGACGTTTCATTTCGCCGGCGGTGCTGATTTGGTGTTGGAATCGGGGAGTTTGTTCCGGCAACATGGTGGGGATCGGTTTTGTTTGGCTATTTTGCCGAGTAATTCTGAGTTGTTGAATCTGTCTGTGATTGGGATTTTGGCTCAACAGAATTATAATGTTGGTTTTGATCTTGAACAAATGAAAGTGTTCTTTCGTAGGATTGATTGTCAACTTCTTGATGAGTAAAGGACTTTTTTTTTAGAAATTATTCAAGTGTCTTCCGTTGATGATTTAGAATTGAAATTTTTGTAAGAATGATTCAATCTTTAAAAATATGGATAAAACTAACCTTTTGTTGAGTCACATGACACTCGCACTTGAGGGACTTCCCTACCATTGTGAAATAGTTAGAGTCCTGATATGATATTCATTTCAATTGCAATCATCCAATCGGATTGCAGATGTGCTCCCACTACGATCCTATAATGGAGTTGACTTCATTTTAC

mRNA sequence

ATGATGATTCTGTTAACTTCTCTCCATCATCTTTTACCTTCCTTAACCTTGGCATTTTATTTGTCAACAGCCGTAATCTCGTCAGCATCGATCACAACAAAGCCTTCACGTTTGGCAACCAAGCTGATCCACCGCAACTCGTATCTACATCCACTCTATGACCCAAATGAGACAGTTGAGGATCAATCGAAGAGAGAGCAAACAAGCTCAATCGAACGCTTTGCTTATCTCGAGTCGAAGATTAAAGAACTGAAGTCTGTTGGTAATGAAGCTCGATCAAGTCTCATTCCTTTCAATCGAGGTAGTGGGTTTCTTGTTAATTTGTCTATTGGTTCGCCACCCGTGACACAGCTCGTAGTGGTCGACACTGGTAGCTCCCTCCTGTGGGTGCAATGTTTGCCTTGTATCAACTGTTTTCAACAATCGAGCTCATGGTTCGATCCTTTGAAATCAGCAAGTTTCAAAACATTGGGTTGCGGGTTTCCTGGGTATAAATACATTAATGGTTACAAATGCAATGGGTTTAATCAAGCTGAGTACAAGTTGAGGTACCTTGGTGGGGATTCCTCACAAGGAATTCTTGCTAAGGAATCACTTCTCTTTGAGACACTTGATGAAGGCAAGATAAAAAAGACGAACTTAACATTTGGGTGTGGTCATATGAACTTCAAAACCAACAATGACGATACCTACAATGGCGTATTTGGATTAGGAGCATATCCCCACATAACAATGGCCACTCAATTAGGCAACAAATTTTCCTATTGCATTGGCGATATCAACAATCCTCTCTACACTCACAATCATCTCGTCTTAGGACAAGGATCTTACATTGAAGGCGATTTCACTCCTCTTCAAATCCACTTTGGCCATTACTATGTCACTTTACAATCCATTAGTGTTGGCTCTAAGACCCTCAAAATCGACCCAAATGCCTTCAAAATCTCATCGGACGGCAGCGGCGGAGTTCTGATCGACTCTGGAATGACCTACACTAAGCTTGCGAATGGTGGGTTTGAATTACTTTATGATGAGATTGTTGATTTGATGAAGGGTTTGCTGGAACGAATCCCAACTCAGAGGAAATTTGAAGGGTTGTGTTTTAAAGGTGTTGTGAGTCGAGATCTTGTTGGGTTTCCGGCTGTGACGTTTCATTTCGCCGGCGGTGCTGATTTGGTGTTGGAATCGGGGAGTTTGTTCCGGCAACATGGTGGGGATCGGTTTTGTTTGGCTATTTTGCCGAGTAATTCTGAGTTGTTGAATCTGTCTGTGATTGGGATTTTGGCTCAACAGAATTATAATGTTGGTTTTGATCTTGAACAAATGAAAGTGTTCTTTCGTAGGATTGATTGTCAACTTCTTGATGAGTAAAGGACTTTTTTTTTAGAAATTATTCAAGTGTCTTCCGTTGATGATTTAGAATTGAAATTTTTGTAAGAATGATTCAATCTTTAAAAATATGGATAAAACTAACCTTTTGTTGAGTCACATGACACTCGCACTTGAGGGACTTCCCTACCATTGTGAAATAGTTAGAGTCCTGATATGATATTCATTTCAATTGCAATCATCCAATCGGATTGCAGATGTGCTCCCACTACGATCCTATAATGGAGTTGACTTCATTTTAC

Coding sequence (CDS)

ATGATGATTCTGTTAACTTCTCTCCATCATCTTTTACCTTCCTTAACCTTGGCATTTTATTTGTCAACAGCCGTAATCTCGTCAGCATCGATCACAACAAAGCCTTCACGTTTGGCAACCAAGCTGATCCACCGCAACTCGTATCTACATCCACTCTATGACCCAAATGAGACAGTTGAGGATCAATCGAAGAGAGAGCAAACAAGCTCAATCGAACGCTTTGCTTATCTCGAGTCGAAGATTAAAGAACTGAAGTCTGTTGGTAATGAAGCTCGATCAAGTCTCATTCCTTTCAATCGAGGTAGTGGGTTTCTTGTTAATTTGTCTATTGGTTCGCCACCCGTGACACAGCTCGTAGTGGTCGACACTGGTAGCTCCCTCCTGTGGGTGCAATGTTTGCCTTGTATCAACTGTTTTCAACAATCGAGCTCATGGTTCGATCCTTTGAAATCAGCAAGTTTCAAAACATTGGGTTGCGGGTTTCCTGGGTATAAATACATTAATGGTTACAAATGCAATGGGTTTAATCAAGCTGAGTACAAGTTGAGGTACCTTGGTGGGGATTCCTCACAAGGAATTCTTGCTAAGGAATCACTTCTCTTTGAGACACTTGATGAAGGCAAGATAAAAAAGACGAACTTAACATTTGGGTGTGGTCATATGAACTTCAAAACCAACAATGACGATACCTACAATGGCGTATTTGGATTAGGAGCATATCCCCACATAACAATGGCCACTCAATTAGGCAACAAATTTTCCTATTGCATTGGCGATATCAACAATCCTCTCTACACTCACAATCATCTCGTCTTAGGACAAGGATCTTACATTGAAGGCGATTTCACTCCTCTTCAAATCCACTTTGGCCATTACTATGTCACTTTACAATCCATTAGTGTTGGCTCTAAGACCCTCAAAATCGACCCAAATGCCTTCAAAATCTCATCGGACGGCAGCGGCGGAGTTCTGATCGACTCTGGAATGACCTACACTAAGCTTGCGAATGGTGGGTTTGAATTACTTTATGATGAGATTGTTGATTTGATGAAGGGTTTGCTGGAACGAATCCCAACTCAGAGGAAATTTGAAGGGTTGTGTTTTAAAGGTGTTGTGAGTCGAGATCTTGTTGGGTTTCCGGCTGTGACGTTTCATTTCGCCGGCGGTGCTGATTTGGTGTTGGAATCGGGGAGTTTGTTCCGGCAACATGGTGGGGATCGGTTTTGTTTGGCTATTTTGCCGAGTAATTCTGAGTTGTTGAATCTGTCTGTGATTGGGATTTTGGCTCAACAGAATTATAATGTTGGTTTTGATCTTGAACAAATGAAAGTGTTCTTTCGTAGGATTGATTGTCAACTTCTTGATGAGTAA

Protein sequence

MMILLTSLHHLLPSLTLAFYLSTAVISSASITTKPSRLATKLIHRNSYLHPLYDPNETVEDQSKREQTSSIERFAYLESKIKELKSVGNEARSSLIPFNRGSGFLVNLSIGSPPVTQLVVVDTGSSLLWVQCLPCINCFQQSSSWFDPLKSASFKTLGCGFPGYKYINGYKCNGFNQAEYKLRYLGGDSSQGILAKESLLFETLDEGKIKKTNLTFGCGHMNFKTNNDDTYNGVFGLGAYPHITMATQLGNKFSYCIGDINNPLYTHNHLVLGQGSYIEGDFTPLQIHFGHYYVTLQSISVGSKTLKIDPNAFKISSDGSGGVLIDSGMTYTKLANGGFELLYDEIVDLMKGLLERIPTQRKFEGLCFKGVVSRDLVGFPAVTFHFAGGADLVLESGSLFRQHGGDRFCLAILPSNSELLNLSVIGILAQQNYNVGFDLEQMKVFFRRIDCQLLDE
Homology
[Close]
BLAST of PI0027280 vs. ExPASy Swiss-Prot
Match: Q3EBM5 (Probable aspartic protease At2g35615 OS=Arabidopsis thaliana OX=3702 GN=At2g35615 PE=3 SV=1)

HSP 1 Score: 188.3 bits (477), Expect = 1.9e-46
Identity = 142/461 (30.80%), Postives = 234/461 (50.76%), Query Frame = 0 

Query: 15  LTLAFYLSTAVISSASITTKPSRLATKLIHRNSYLHPLYDPNETVEDQSKREQTSSIERF 74
           + L F+L  +V  S+S    P   + +LIHR+S L P+Y+P  TV D+       S+ R 
Sbjct: 5   ILLCFFLFFSVTLSSS--GHPKNFSVELIHRDSPLSPIYNPQITVTDRLNAAFLRSVSRS 64

Query: 75  AYLESKIKELKSVGNEARSSLIPFNRGSGFLVNLSIGSPPVTQLVVVDTGSSLLWVQCLP 134
                ++ +      + +S LI       F ++++IG+PP+    + DTGS L WVQC P
Sbjct: 65  RRFNHQLSQ-----TDLQSGLI--GADGEFFMSITIGTPPIKVFAIADTGSDLTWVQCKP 124

Query: 135 CINCFQQSSSWFDPLKSASFKTLGCGFPGYKYINGYK--CNGFNQAEYKLRYLGGDS--S 194
           C  C++++   FD  KS+++K+  C     + ++  +  C+  N    K RY  GD   S
Sbjct: 125 CQQCYKENGPIFDKKKSSTYKSEPCDSRNCQALSSTERGCDESNNI-CKYRYSYGDQSFS 184

Query: 195 QGILAKESLLFETLDEGKIKKTNLTFGCGHMNFKTNNDDTYNGVFGLGAYPHITMATQLG 254
           +G +A E++  ++     +      FGCG+ N  T  D+T +G+ GLG   H+++ +QLG
Sbjct: 185 KGDVATETVSIDSASGSPVSFPGTVFGCGYNNGGT-FDETGSGIIGLGG-GHLSLISQLG 244

Query: 255 N----KFSYCIGDINNPLYTHNHLVLGQG-----SYIEGD----FTPL--QIHFGHYYVT 314
           +    KFSYC+   +    T+   V+  G     S +  D     TPL  +    +YY+T
Sbjct: 245 SSISKKFSYCLS--HKSATTNGTSVINLGTNSIPSSLSKDSGVVSTPLVDKEPLTYYYLT 304

Query: 315 LQSISVGSKTL-----KIDPNAFKISSDGSGGVLIDSGMTYTKLANGGFELLYDEIVDLM 374
           L++ISVG K +       +PN   I S+ SG ++IDSG T T L  G F+     + + +
Sbjct: 305 LEAISVGKKKIPYTGSSYNPNDDGILSETSGNIIIDSGTTLTLLEAGFFDKFSSAVEESV 364

Query: 375 KGLLERIPTQRKFEGLCFKGVVSRDLVGFPAVTFHFAGGADLVLESGSLFRQHGGDRFCL 434
            G  +R+   +     CFK   +   +G P +T HF  GAD+ L   + F +   D  CL
Sbjct: 365 TG-AKRVSDPQGLLSHCFKSGSAE--IGLPEITVHFT-GADVRLSPINAFVKLSEDMVCL 424

Query: 435 AILPSNSELLNLSVIGILAQQNYNVGFDLEQMKVFFRRIDC 452
           +++P+      +++ G  AQ ++ VG+DLE   V F+ +DC
Sbjct: 425 SMVPTT----EVAIYGNFAQMDFLVGYDLETRTVSFQHMDC 443

[Close]
BLAST of PI0027280 vs. ExPASy Swiss-Prot
Match: Q6XBF8 (Aspartic proteinase CDR1 OS=Arabidopsis thaliana OX=3702 GN=CDR1 PE=1 SV=1)

HSP 1 Score: 179.5 bits (454), Expect = 8.6e-44
Identity = 140/456 (30.70%), Postives = 212/456 (46.49%), Query Frame = 0 

Query: 12  LPSLTLAFYLSTAVISS---ASITTKPS-RLATKLIHRNSYLHPLYDPNETVEDQSKREQ 71
           + SL  +  LS  ++SS   ++   KP       LIHR+S   P Y+P ET   + +   
Sbjct: 1   MASLFSSVLLSLCLLSSLFLSNANAKPKLGFTADLIHRDSPKSPFYNPMETSSQRLRNAI 60

Query: 72  TSSIERFAYLESKIKELKSVGNEARSSLIPFNRGSGFLVNLSIGSPPVTQLVVVDTGSSL 131
             S+ R  +   K        N  +  +   +    +L+N+SIG+PP   + + DTGS L
Sbjct: 61  HRSVNRVFHFTEK-------DNTPQPQIDLTSNSGEYLMNVSIGTPPFPIMAIADTGSDL 120

Query: 132 LWVQCLPCINCFQQSSSWFDPLKSASFKTLGCGFPGYKYI-NGYKCN-GFNQAEYKLRYL 191
           LW QC PC +C+ Q    FDP  S+++K + C       + N   C+   N   Y L Y 
Sbjct: 121 LWTQCAPCDDCYTQVDPLFDPKTSSTYKDVSCSSSQCTALENQASCSTNDNTCSYSLSYG 180

Query: 192 GGDSSQGILAKESLLFETLDEGKIKKTNLTFGCGHMNFKTNNDDTYNGVFGLGAYPHITM 251
               ++G +A ++L   + D   ++  N+  GCGH N  T N    +G+ GLG  P +++
Sbjct: 181 DNSYTKGNIAVDTLTLGSSDTRPMQLKNIIIGCGHNNAGTFNKKG-SGIVGLGGGP-VSL 240

Query: 252 ATQLGN----KFSYCIGDINNPLYTHNHLVLGQGSYIEGD---FTPLQIHFGH---YYVT 311
             QLG+    KFSYC+  + +     + +  G  + + G     TPL         YY+T
Sbjct: 241 IKQLGDSIDGKFSYCLVPLTSKKDQTSKINFGTNAIVSGSGVVSTPLIAKASQETFYYLT 300

Query: 312 LQSISVGSKTLKIDPNAFKISSDGSGGVLIDSGMTYTKLANGGFELLYDEIVDLMKGLLE 371
           L+SISVGSK ++   +    S    G ++IDSG T T L        Y E+ D +   ++
Sbjct: 301 LKSISVGSKQIQYSGSD---SESSEGNIIIDSGTTLTLLPTE----FYSELEDAVASSID 360

Query: 372 RIPTQRKFEGLCFKGVVSRDLVGFPAVTFHFAGGADLVLESGSLFRQHGGDRFCLAILPS 431
               Q    GL      + DL   P +T HF  GAD+ L+S + F Q   D  C A   S
Sbjct: 361 AEKKQDPQSGLSLCYSATGDL-KVPVITMHF-DGADVKLDSSNAFVQVSEDLVCFAFRGS 420

Query: 432 NSELLNLSVIGILAQQNYNVGFDLEQMKVFFRRIDC 452
            S     S+ G +AQ N+ VG+D     V F+  DC
Sbjct: 421 PS----FSIYGNVAQMNFLVGYDTVSKTVSFKPTDC 434

[Close]
BLAST of PI0027280 vs. ExPASy Swiss-Prot
Match: Q766C3 (Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 SV=1)

HSP 1 Score: 172.9 bits (437), Expect = 8.1e-42
Identity = 122/358 (34.08%), Postives = 175/358 (48.88%), Query Frame = 0 

Query: 104 FLVNLSIGSPPVTQLVVVDTGSSLLWVQCLPCINCFQQSSSWFDPLKSASFKTLGCGFPG 163
           +L+NLSIG+P      ++DTGS L+W QC PC  CF QS+  F+P  S+SF TL C    
Sbjct: 95  YLMNLSIGTPAQPFSAIMDTGSDLIWTQCQPCTQCFNQSTPIFNPQGSSSFSTLPCSSQL 154

Query: 164 YKYINGYKCNGFNQAEYKLRYLGGDSSQGILAKESLLFETLDEGKIKKTNLTFGCGHMN- 223
            + ++   C+  N  +Y   Y  G  +QG +  E+L F     G +   N+TFGCG  N 
Sbjct: 155 CQALSSPTCSN-NFCQYTYGYGDGSETQGSMGTETLTF-----GSVSIPNITFGCGENNQ 214

Query: 224 -FKTNNDDTYNGVFGLGAYPHITMATQLG-NKFSYCIGDINNPLYTHNHLVLGQ--GSYI 283
            F   N     G+ G+G  P +++ +QL   KFSYC+  I +   T ++L+LG    S  
Sbjct: 215 GFGQGNG---AGLVGMGRGP-LSLPSQLDVTKFSYCMTPIGSS--TPSNLLLGSLANSVT 274

Query: 284 EGDFTPLQIHFGH----YYVTLQSISVGSKTLKIDPNAFKI-SSDGSGGVLIDSGMTYTK 343
            G      I        YY+TL  +SVGS  L IDP+AF + S++G+GG++IDSG T T 
Sbjct: 275 AGSPNTTLIQSSQIPTFYYITLNGLSVGSTRLPIDPSAFALNSNNGTGGIIIDSGTTLTY 334

Query: 344 LANGGFELLYDEIVDLMKGLLERIPTQRKFEGLCFKGVVSRDLVGFPAVTFHFAGGADLV 403
             N  ++ +  E +  +   L  +        LCF+       +  P    HF GG DL 
Sbjct: 335 FVNNAYQSVRQEFISQIN--LPVVNGSSSGFDLCFQTPSDPSNLQIPTFVMHFDGG-DLE 394

Query: 404 LESGSLFRQHGGDRFCLAILPSNSELLNLSVIGILAQQNYNVGFDLEQMKVFFRRIDC 452
           L S + F        CLA+    S    +S+ G + QQN  V +D     V F    C
Sbjct: 395 LPSENYFISPSNGLICLAM---GSSSQGMSIFGNIQQQNMLVVYDTGNSVVSFASAQC 434

[Close]
BLAST of PI0027280 vs. ExPASy Swiss-Prot
Match: Q766C2 (Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 SV=1)

HSP 1 Score: 163.3 bits (412), Expect = 6.4e-39
Identity = 129/441 (29.25%), Postives = 200/441 (45.35%), Query Frame = 0 

Query: 21  LSTAVISSASITTKPSRLATKLIHRNSYLHP-LYDPNETVEDQSKREQTSSIER-FAYLE 80
           L  A++S+    T  +   T L H      P L    E V+      +   I+R     E
Sbjct: 10  LGLAIVSAIVAPTSSTSRGTLLHHGQKRPQPGLRVDLEQVDSGKNLTKYELIKRAIKRGE 69

Query: 81  SKIKELKSVGNEARSSLIPFNRGSG-FLVNLSIGSPPVTQLVVVDTGSSLLWVQCLPCIN 140
            +++ + ++   +     P   G G +L+N++IG+P  +   ++DTGS L+W QC PC  
Sbjct: 70  RRMRSINAMLQSSSGIETPVYAGDGEYLMNVAIGTPDSSFSAIMDTGSDLIWTQCEPCTQ 129

Query: 141 CFQQSSSWFDPLKSASFKTLGCGFPGYKYINGYKCNGFNQAEYKLRYLGGDSSQGILAKE 200
           CF Q +  F+P  S+SF TL C     + +    CN  N+ +Y   Y  G ++QG +A E
Sbjct: 130 CFSQPTPIFNPQDSSSFSTLPCESQYCQDLPSETCNN-NECQYTYGYGDGSTTQGYMATE 189

Query: 201 SLLFETLDEGKIKKTNLTFGCGHMN--FKTNNDDTYNGVFGLGAYPHITMATQLG-NKFS 260
           +  FET         N+ FGCG  N  F   N     G+ G+G  P +++ +QLG  +FS
Sbjct: 190 TFTFET-----SSVPNIAFGCGEDNQGFGQGNG---AGLIGMGWGP-LSLPSQLGVGQFS 249

Query: 261 YCIGDINNPLYTHNHLVLGQGSYIEGDFTPLQIHFG----HYYVTLQSISVGSKTLKIDP 320
           YC+    +   +   L        EG  +   IH      +YY+TLQ I+VG   L I  
Sbjct: 250 YCMTSYGSSSPSTLALGSAASGVPEGSPSTTLIHSSLNPTYYYITLQGITVGGDNLGIPS 309

Query: 321 NAFKISSDGSGGVLIDSGMTYTKLANGGFELLYDEIVDLMKGLLERIPTQRKFEGLCFKG 380
           + F++  DG+GG++IDSG T T L    +  +     D +   L  +         CF+ 
Sbjct: 310 STFQLQDDGTGGMIIDSGTTLTYLPQDAYNAVAQAFTDQIN--LPTVDESSSGLSTCFQQ 369

Query: 381 VVSRDLVGFPAVTFHFAGGADLVLESGSLFRQHGGDRFCLAILPSNSELLNLSVIGILAQ 440
                 V  P ++  F GG   + E   L     G   CLA+   +S  L +S+ G + Q
Sbjct: 370 PSDGSTVQVPEISMQFDGGVLNLGEQNILISPAEG-VICLAM--GSSSQLGISIFGNIQQ 429

Query: 441 QNYNVGFDLEQMKVFFRRIDC 452
           Q   V +DL+ + V F    C
Sbjct: 430 QETQVLYDLQNLAVSFVPTQC 435

[Close]
BLAST of PI0027280 vs. ExPASy Swiss-Prot
Match: Q9SV77 (Aspartyl protease UND OS=Arabidopsis thaliana OX=3702 GN=UND PE=2 SV=1)

HSP 1 Score: 156.0 bits (393), Expect = 1.0e-36
Identity = 112/360 (31.11%), Postives = 176/360 (48.89%), Query Frame = 0 

Query: 100 RGSGFLVNLSIGSPPVTQLVVVDTGSSLLWVQCLPCINCF-QQSSSWFDPLKSASFKTLG 159
           RG  F+  +  GSP   Q + +DTGSSL W QC PC +C+ Q+    + P  S +++   
Sbjct: 54  RGLAFMAEIHFGSPQKKQFLHMDTGSSLTWTQCFPCSDCYAQKIYPKYRPAASITYRDAM 113

Query: 160 CGFPGYKYINGYKCNGFNQ-AEYKLRYLGGDSSQGILAKESLLFETLDEGKIKKTNLTFG 219
           C     K    +  +   +   Y+  YL   + +G LA+E +  +T D G  +   + FG
Sbjct: 114 CEDSHPKSNPHFAFDPLTRICTYQQHYLDETNIKGTLAQEMITVDTHDGGFKRVHGVYFG 173

Query: 220 CGHMNFKTNNDDTY---NGVFGLGAYPHITMATQLGNKFSYCIGDINNPLYTHNHLVLGQ 279
           C      T +D +Y    G+ GLG   + ++  + G+KFS+C+G+I+ P  +HN L+LG 
Sbjct: 174 C-----NTLSDGSYFTGTGILGLGVGKY-SIIGEFGSKFSFCLGEISEPKASHN-LILGD 233

Query: 280 GSYIEGDFTPLQIHFGHYYVTLQSISVGSKTLKIDPNAFKISSDGSGGVLIDSGMTYTKL 339
           G+ ++G  T + I  GH    L+SI VG           +I+ D    V +D+G T + L
Sbjct: 234 GANVQGHPTVINITEGHTIFQLESIIVGE----------EITLDDPVQVFVDTGSTLSHL 293

Query: 340 ANGGFELLYDEIVDLMKGLLERIPTQRKFEGLCFKGVVSRDLVGFPAVTFHFAGGADLVL 399
           +      LY + VD    L+   P   +   LC+K      L     V F F  GA+L +
Sbjct: 294 STN----LYYKFVDAFDDLIGSRPLSYE-PTLCYKADTIERLEKMD-VGFKFDVGAELSV 353

Query: 400 ESGSLFRQHGGDRF-CLAILPSNSELLNLSVIGILAQQNYNVGFDLEQMKVFFRRIDCQL 454
              ++F Q G     CLAI  +N E  +  +IG++A Q YNVG+DL     +  + DC +
Sbjct: 354 NIHNIFIQQGPPEIRCLAI-QNNKESFSHVIIGVIAMQGYNVGYDLSAKTAYINKQDCDM 389

[Close]
BLAST of PI0027280 vs. ExPASy TrEMBL
Match: A0A5D3DZ20 (Peptidase A1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold629G002350 PE=3 SV=1)

HSP 1 Score: 892.5 bits (2305), Expect = 7.4e-256
Identity = 443/456 (97.15%), Postives = 448/456 (98.25%), Query Frame = 0 

Query: 1   MMILLTSLHHLLPSLTLAFYLSTAVISSASITTKPSRLATKLIHRNSYLHPLYDPNETVE 60
           MMILL SLHHLLPSLTLAFYLSTA+I S SI TKPSRLATKLIHRNSYLHPLYDPNETVE
Sbjct: 390 MMILLASLHHLLPSLTLAFYLSTAIILSTSIMTKPSRLATKLIHRNSYLHPLYDPNETVE 449

Query: 61  DQSKREQTSSIERFAYLESKIKELKSVGNEARSSLIPFNRGSGFLVNLSIGSPPVTQLVV 120
           D+SKREQ SSIERFA+LESKIKELKSVGNEARSSLIPFNRGSGFLVNLSIGSPPVTQLVV
Sbjct: 450 DRSKREQASSIERFAFLESKIKELKSVGNEARSSLIPFNRGSGFLVNLSIGSPPVTQLVV 509

Query: 121 VDTGSSLLWVQCLPCINCFQQSSSWFDPLKSASFKTLGCGFPGYKYINGYKCNGFNQAEY 180
           VDTGSSLLWVQCLPCINCFQQS+SWFDPLKSASFKTLGCGFPGY YINGYKCNG NQAEY
Sbjct: 510 VDTGSSLLWVQCLPCINCFQQSTSWFDPLKSASFKTLGCGFPGYNYINGYKCNG-NQAEY 569

Query: 181 KLRYLGGDSSQGILAKESLLFETLDEGKIKKTNLTFGCGHMNFKTNNDDTYNGVFGLGAY 240
           KLRYLGGDSSQGILAKESLLFETLDEGKIKKTNLTFGCGHMNFKTNNDDTYNGVFGLGAY
Sbjct: 570 KLRYLGGDSSQGILAKESLLFETLDEGKIKKTNLTFGCGHMNFKTNNDDTYNGVFGLGAY 629

Query: 241 PHITMATQLGNKFSYCIGDINNPLYTHNHLVLGQGSYIEGDFTPLQIHFGHYYVTLQSIS 300
           PHITMATQLGNKFSYCIGDINNPLYTHNHLVLGQGSYIEGD TPLQIHFGHYYVTLQ+IS
Sbjct: 630 PHITMATQLGNKFSYCIGDINNPLYTHNHLVLGQGSYIEGDSTPLQIHFGHYYVTLQTIS 689

Query: 301 VGSKTLKIDPNAFKISSDGSGGVLIDSGMTYTKLANGGFELLYDEIVDLMKGLLERIPTQ 360
           VGSKTLKIDPNAFKISSDGSGGVLIDSGMTYTKLANGGFELLYDEIVDLMKGLLERIPTQ
Sbjct: 690 VGSKTLKIDPNAFKISSDGSGGVLIDSGMTYTKLANGGFELLYDEIVDLMKGLLERIPTQ 749

Query: 361 RKFEGLCFKGVVSRDLVGFPAVTFHFAGGADLVLESGSLFRQHGGDRFCLAILPSNSELL 420
           RKFEGLCFKGVVSRDLVGFPAVTFHFAGGADLVLESGSLFRQHGGDRFCLAILPSNSELL
Sbjct: 750 RKFEGLCFKGVVSRDLVGFPAVTFHFAGGADLVLESGSLFRQHGGDRFCLAILPSNSELL 809

Query: 421 NLSVIGILAQQNYNVGFDLEQMKVFFRRIDCQLLDE 457
           NLSVIGILAQQNYNVGFDLEQMKVFFRRIDCQLLDE
Sbjct: 810 NLSVIGILAQQNYNVGFDLEQMKVFFRRIDCQLLDE 844

[Close]
BLAST of PI0027280 vs. ExPASy TrEMBL
Match: A0A5A7UU11 (Aspartic proteinase CDR1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold274G005510 PE=3 SV=1)

HSP 1 Score: 892.5 bits (2305), Expect = 7.4e-256
Identity = 443/456 (97.15%), Postives = 449/456 (98.46%), Query Frame = 0 

Query: 1   MMILLTSLHHLLPSLTLAFYLSTAVISSASITTKPSRLATKLIHRNSYLHPLYDPNETVE 60
           MMILL SLHHLLPSLTLAFYLSTA+I S SITTKPSRLATKLIHRNSYLHPLYDPNETVE
Sbjct: 390 MMILLASLHHLLPSLTLAFYLSTAIILSTSITTKPSRLATKLIHRNSYLHPLYDPNETVE 449

Query: 61  DQSKREQTSSIERFAYLESKIKELKSVGNEARSSLIPFNRGSGFLVNLSIGSPPVTQLVV 120
           D+SKREQ SSIERFA+LESKIKELKSVGNEARSSLIPFNRGSGFLVNLSIGSPPVTQLVV
Sbjct: 450 DRSKREQASSIERFAFLESKIKELKSVGNEARSSLIPFNRGSGFLVNLSIGSPPVTQLVV 509

Query: 121 VDTGSSLLWVQCLPCINCFQQSSSWFDPLKSASFKTLGCGFPGYKYINGYKCNGFNQAEY 180
           VDTGSSLLWVQCLPCINCFQQS+SWFDPLKSASFKTLGCGFPGY YINGYKCNG NQAEY
Sbjct: 510 VDTGSSLLWVQCLPCINCFQQSTSWFDPLKSASFKTLGCGFPGYNYINGYKCNG-NQAEY 569

Query: 181 KLRYLGGDSSQGILAKESLLFETLDEGKIKKTNLTFGCGHMNFKTNNDDTYNGVFGLGAY 240
           KLRYLGGDSSQGILAKESLLFETLDEGKIKKTNLTFGCGHMNFKTNNDDTYNGVFGLGAY
Sbjct: 570 KLRYLGGDSSQGILAKESLLFETLDEGKIKKTNLTFGCGHMNFKTNNDDTYNGVFGLGAY 629

Query: 241 PHITMATQLGNKFSYCIGDINNPLYTHNHLVLGQGSYIEGDFTPLQIHFGHYYVTLQSIS 300
           P+ITMATQLGNKFSYCIGDINNPLYTHNHLVLGQGSYIEGD TPLQIHFGHYYVTLQ+IS
Sbjct: 630 PYITMATQLGNKFSYCIGDINNPLYTHNHLVLGQGSYIEGDSTPLQIHFGHYYVTLQTIS 689

Query: 301 VGSKTLKIDPNAFKISSDGSGGVLIDSGMTYTKLANGGFELLYDEIVDLMKGLLERIPTQ 360
           VGSKTLKIDPNAFKISSDGSGGVLIDSGMTYTKLANGGFELLYDEIVDLMKGLLERIPTQ
Sbjct: 690 VGSKTLKIDPNAFKISSDGSGGVLIDSGMTYTKLANGGFELLYDEIVDLMKGLLERIPTQ 749

Query: 361 RKFEGLCFKGVVSRDLVGFPAVTFHFAGGADLVLESGSLFRQHGGDRFCLAILPSNSELL 420
           RKFEGLCFKGVVSRDLVGFPAVTFHFAGGADLVLESGSLFRQHGGDRFCLAILPSNSELL
Sbjct: 750 RKFEGLCFKGVVSRDLVGFPAVTFHFAGGADLVLESGSLFRQHGGDRFCLAILPSNSELL 809

Query: 421 NLSVIGILAQQNYNVGFDLEQMKVFFRRIDCQLLDE 457
           NLSVIGILAQQNYNVGFDLEQMKVFFRRIDCQLLDE
Sbjct: 810 NLSVIGILAQQNYNVGFDLEQMKVFFRRIDCQLLDE 844

[Close]
BLAST of PI0027280 vs. ExPASy TrEMBL
Match: A0A0A0LUP5 (Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G424350 PE=3 SV=1)

HSP 1 Score: 888.3 bits (2294), Expect = 1.4e-254
Identity = 439/456 (96.27%), Postives = 445/456 (97.59%), Query Frame = 0 

Query: 1   MMILLTSLHHLLPSLTLAFYLSTAVISSASITTKPSRLATKLIHRNSYLHPLYDPNETVE 60
           MMILL SLHHLLPSLTLAFYLSTA+ISS  ITTKPSRLATKLIHRNSYLHPLYD NETVE
Sbjct: 1   MMILLASLHHLLPSLTLAFYLSTAIISSTLITTKPSRLATKLIHRNSYLHPLYDQNETVE 60

Query: 61  DQSKREQTSSIERFAYLESKIKELKSVGNEARSSLIPFNRGSGFLVNLSIGSPPVTQLVV 120
           D+SKREQTSSIERF +LESKIKELKSVGNEARSSLIPFNRGSGFLVNLSIGSPPVTQLVV
Sbjct: 61  DRSKREQTSSIERFDFLESKIKELKSVGNEARSSLIPFNRGSGFLVNLSIGSPPVTQLVV 120

Query: 121 VDTGSSLLWVQCLPCINCFQQSSSWFDPLKSASFKTLGCGFPGYKYINGYKCNGFNQAEY 180
           VDTGSSLLWVQCLPCINCFQQS+SWFDPLKS SFKTLGCGFPGY YINGYKCN FNQAEY
Sbjct: 121 VDTGSSLLWVQCLPCINCFQQSTSWFDPLKSVSFKTLGCGFPGYNYINGYKCNRFNQAEY 180

Query: 181 KLRYLGGDSSQGILAKESLLFETLDEGKIKKTNLTFGCGHMNFKTNNDDTYNGVFGLGAY 240
           KLRYLGGDSSQGILAKESLLFETLDEGKIKK+N+TFGCGHMN KTNNDD YNGVFGLGAY
Sbjct: 181 KLRYLGGDSSQGILAKESLLFETLDEGKIKKSNITFGCGHMNIKTNNDDAYNGVFGLGAY 240

Query: 241 PHITMATQLGNKFSYCIGDINNPLYTHNHLVLGQGSYIEGDFTPLQIHFGHYYVTLQSIS 300
           PHITMATQLGNKFSYCIGDINNPLYTHNHLVLGQGSYIEGD TPLQIHFGHYYVTLQSIS
Sbjct: 241 PHITMATQLGNKFSYCIGDINNPLYTHNHLVLGQGSYIEGDSTPLQIHFGHYYVTLQSIS 300

Query: 301 VGSKTLKIDPNAFKISSDGSGGVLIDSGMTYTKLANGGFELLYDEIVDLMKGLLERIPTQ 360
           VGSKTLKIDPNAFKISSDGSGGVLIDSGMTYTKLANGGFELLYDEIVDLMKGLLERIPTQ
Sbjct: 301 VGSKTLKIDPNAFKISSDGSGGVLIDSGMTYTKLANGGFELLYDEIVDLMKGLLERIPTQ 360

Query: 361 RKFEGLCFKGVVSRDLVGFPAVTFHFAGGADLVLESGSLFRQHGGDRFCLAILPSNSELL 420
           RKFEGLCFKGVVSRDLVGFPAVTFHFAGGADLVLESGSLFRQHGGDRFCLAILPSNSELL
Sbjct: 361 RKFEGLCFKGVVSRDLVGFPAVTFHFAGGADLVLESGSLFRQHGGDRFCLAILPSNSELL 420

Query: 421 NLSVIGILAQQNYNVGFDLEQMKVFFRRIDCQLLDE 457
           NLSVIGILAQQNYNVGFDLEQMKVFFRRIDCQLLDE
Sbjct: 421 NLSVIGILAQQNYNVGFDLEQMKVFFRRIDCQLLDE 456

[Close]
BLAST of PI0027280 vs. ExPASy TrEMBL
Match: A0A1S3BY28 (aspartic proteinase CDR1-like OS=Cucumis melo OX=3656 GN=LOC103494349 PE=3 SV=1)

HSP 1 Score: 879.0 bits (2270), Expect = 8.4e-252
Identity = 441/472 (93.43%), Postives = 448/472 (94.92%), Query Frame = 0 

Query: 1   MMILLTSLHHLLPSLTLAFYLSTAVISSASITTKPSRLATKLIHRNSYLHPLYDPNETVE 60
           MMILL SLHHLLPSLTLAFYLSTA+I S SITTKPSRLATKLIHRNSYLHPLYDPNETVE
Sbjct: 14  MMILLASLHHLLPSLTLAFYLSTAIILSTSITTKPSRLATKLIHRNSYLHPLYDPNETVE 73

Query: 61  DQSKREQTSSIERFAYLESKIKELKSVGNEARSSLIPFNRGSGFLVNLSIGSPPVTQLVV 120
           D+SKREQ SSIERFA+LESKIKELKSVGNEARSSLIPFNRGSGFLVNLSIGSPPVTQLVV
Sbjct: 74  DRSKREQASSIERFAFLESKIKELKSVGNEARSSLIPFNRGSGFLVNLSIGSPPVTQLVV 133

Query: 121 VDTGSSLLWVQCLPCINCFQQSSSWFDPLKSASFKTLGCGFPGYKYINGYKCNGFNQAEY 180
           VDTGSSLLWVQCLPCINCFQQS+SWFDPLKSASFKTLGCGFPGY YINGYKCNG NQAEY
Sbjct: 134 VDTGSSLLWVQCLPCINCFQQSTSWFDPLKSASFKTLGCGFPGYNYINGYKCNG-NQAEY 193

Query: 181 KLRYLGGDSSQGILAKESLLFETLDEGKI----------------KKTNLTFGCGHMNFK 240
           KLRYLGGDSSQGILAKESLLFETLDEG +                KKTNLTFGCGHMNFK
Sbjct: 194 KLRYLGGDSSQGILAKESLLFETLDEGGVFQYNALFQHKQVNKQNKKTNLTFGCGHMNFK 253

Query: 241 TNNDDTYNGVFGLGAYPHITMATQLGNKFSYCIGDINNPLYTHNHLVLGQGSYIEGDFTP 300
           TNNDDTYNGVFGLGAYP+ITMATQLGNKFSYCIGDINNPLYTHNHLVLGQGSYIEGD TP
Sbjct: 254 TNNDDTYNGVFGLGAYPYITMATQLGNKFSYCIGDINNPLYTHNHLVLGQGSYIEGDSTP 313

Query: 301 LQIHFGHYYVTLQSISVGSKTLKIDPNAFKISSDGSGGVLIDSGMTYTKLANGGFELLYD 360
           LQIHFGHYYVTLQ+ISVGSKTLKIDPNAFKISSDGSGGVLIDSGMTYTKLANGGFELLYD
Sbjct: 314 LQIHFGHYYVTLQTISVGSKTLKIDPNAFKISSDGSGGVLIDSGMTYTKLANGGFELLYD 373

Query: 361 EIVDLMKGLLERIPTQRKFEGLCFKGVVSRDLVGFPAVTFHFAGGADLVLESGSLFRQHG 420
           EIVDLMKGLLERIPTQRKFEGLCFKGVVSRDLVGFPAVTFHFAGGADLVLESGSLFRQHG
Sbjct: 374 EIVDLMKGLLERIPTQRKFEGLCFKGVVSRDLVGFPAVTFHFAGGADLVLESGSLFRQHG 433

Query: 421 GDRFCLAILPSNSELLNLSVIGILAQQNYNVGFDLEQMKVFFRRIDCQLLDE 457
           GDRFCLAILPSNSELLNLSVIGILAQQNYNVGFDLEQMKVFFRRIDCQLLDE
Sbjct: 434 GDRFCLAILPSNSELLNLSVIGILAQQNYNVGFDLEQMKVFFRRIDCQLLDE 484

[Close]
BLAST of PI0027280 vs. ExPASy TrEMBL
Match: A0A6J1C0L1 (probable aspartic protease At2g35615 OS=Momordica charantia OX=3673 GN=LOC111006977 PE=3 SV=1)

HSP 1 Score: 792.3 bits (2045), Expect = 1.0e-225
Identity = 384/457 (84.03%), Postives = 416/457 (91.03%), Query Frame = 0 

Query: 1   MMILLTSLHHLLPSLTLAFYLSTAVISSASITTKPSRLATKLIHRNSYLHPLYDPNETVE 60
           M+ LL SLHHLLP LT AFYLSTAVISS  +TTKPSRL TKLIHRNSYLHPLYDPNETVE
Sbjct: 1   MVTLLLSLHHLLPFLTSAFYLSTAVISSTDVTTKPSRLVTKLIHRNSYLHPLYDPNETVE 60

Query: 61  DQSKREQTSSIERFAYLESKIKELKSV-GNEARSSLIPFNRGSGFLVNLSIGSPPVTQLV 120
           D+SKRE+TSS ERFAYLESKIKEL SV GNEAR++LIPFN+GSGFLVN SIG PPVTQL 
Sbjct: 61  DRSKREETSSTERFAYLESKIKELNSVGGNEARANLIPFNQGSGFLVNFSIGQPPVTQLA 120

Query: 121 VVDTGSSLLWVQCLPCINCFQQSSSWFDPLKSASFKTLGCGFPGYKYINGYKCNGFNQAE 180
           VVDTGSSLLWVQCLPC+NCF+QS SWFDPLKS SFK L C FPG+ Y+ GYKCN F+QAE
Sbjct: 121 VVDTGSSLLWVQCLPCVNCFRQSGSWFDPLKSWSFKILDCDFPGHNYVRGYKCNDFHQAE 180

Query: 181 YKLRYLGGDSSQGILAKESLLFETLDEGKIKKTNLTFGCGHMNFKTNNDDTYNGVFGLGA 240
           YKLRYLGGD+S+GILAKESLLFET DEGKI+K NLTFGCGHMN KTN DDTYNGVFGLG 
Sbjct: 181 YKLRYLGGDTSEGILAKESLLFETPDEGKIRKANLTFGCGHMNTKTNRDDTYNGVFGLGG 240

Query: 241 YPHITMATQLGNKFSYCIGDINNPLYTHNHLVLGQGSYIEGDFTPLQIHFGHYYVTLQSI 300
           YPHITMATQLGNKFSYCIGDI +PLY HNHL LG G++IEGD TPLQI FGHYYV+L+ I
Sbjct: 241 YPHITMATQLGNKFSYCIGDITDPLYAHNHLFLGHGAFIEGDSTPLQIIFGHYYVSLEGI 300

Query: 301 SVGSKTLKIDPNAFKISSDGSGGVLIDSGMTYTKLANGGFELLYDEIVDLMKGLLERIPT 360
           SVGSK LKIDPNAF+++SDG GGVLIDSGMTYTKL NGGFELL+DEI DLMKG+LERIPT
Sbjct: 301 SVGSKRLKIDPNAFQMTSDGRGGVLIDSGMTYTKLTNGGFELLFDEIADLMKGVLERIPT 360

Query: 361 QRKFEGLCFKGVVSRDLVGFPAVTFHFAGGADLVLESGSLFRQHGGDRFCLAILPSNSEL 420
           +RKFEGLC+KGVV RDLVG P VTFHFAGGADLVLESGSLFRQHGGDRFCLA+LPSNSE+
Sbjct: 361 RRKFEGLCYKGVVGRDLVGLPPVTFHFAGGADLVLESGSLFRQHGGDRFCLAVLPSNSEM 420

Query: 421 LNLSVIGILAQQNYNVGFDLEQMKVFFRRIDCQLLDE 457
           +NLSVIG+LAQQNYNVGFDLEQMKVFFRRIDCQLL +
Sbjct: 421 MNLSVIGVLAQQNYNVGFDLEQMKVFFRRIDCQLLGD 457

[Close]
BLAST of PI0027280 vs. NCBI nr
Match: KAA0058227.1 (aspartic proteinase CDR1-like [Cucumis melo var. makuwa])

HSP 1 Score: 892.5 bits (2305), Expect = 1.5e-255
Identity = 443/456 (97.15%), Postives = 449/456 (98.46%), Query Frame = 0 

Query: 1   MMILLTSLHHLLPSLTLAFYLSTAVISSASITTKPSRLATKLIHRNSYLHPLYDPNETVE 60
           MMILL SLHHLLPSLTLAFYLSTA+I S SITTKPSRLATKLIHRNSYLHPLYDPNETVE
Sbjct: 390 MMILLASLHHLLPSLTLAFYLSTAIILSTSITTKPSRLATKLIHRNSYLHPLYDPNETVE 449

Query: 61  DQSKREQTSSIERFAYLESKIKELKSVGNEARSSLIPFNRGSGFLVNLSIGSPPVTQLVV 120
           D+SKREQ SSIERFA+LESKIKELKSVGNEARSSLIPFNRGSGFLVNLSIGSPPVTQLVV
Sbjct: 450 DRSKREQASSIERFAFLESKIKELKSVGNEARSSLIPFNRGSGFLVNLSIGSPPVTQLVV 509

Query: 121 VDTGSSLLWVQCLPCINCFQQSSSWFDPLKSASFKTLGCGFPGYKYINGYKCNGFNQAEY 180
           VDTGSSLLWVQCLPCINCFQQS+SWFDPLKSASFKTLGCGFPGY YINGYKCNG NQAEY
Sbjct: 510 VDTGSSLLWVQCLPCINCFQQSTSWFDPLKSASFKTLGCGFPGYNYINGYKCNG-NQAEY 569

Query: 181 KLRYLGGDSSQGILAKESLLFETLDEGKIKKTNLTFGCGHMNFKTNNDDTYNGVFGLGAY 240
           KLRYLGGDSSQGILAKESLLFETLDEGKIKKTNLTFGCGHMNFKTNNDDTYNGVFGLGAY
Sbjct: 570 KLRYLGGDSSQGILAKESLLFETLDEGKIKKTNLTFGCGHMNFKTNNDDTYNGVFGLGAY 629

Query: 241 PHITMATQLGNKFSYCIGDINNPLYTHNHLVLGQGSYIEGDFTPLQIHFGHYYVTLQSIS 300
           P+ITMATQLGNKFSYCIGDINNPLYTHNHLVLGQGSYIEGD TPLQIHFGHYYVTLQ+IS
Sbjct: 630 PYITMATQLGNKFSYCIGDINNPLYTHNHLVLGQGSYIEGDSTPLQIHFGHYYVTLQTIS 689

Query: 301 VGSKTLKIDPNAFKISSDGSGGVLIDSGMTYTKLANGGFELLYDEIVDLMKGLLERIPTQ 360
           VGSKTLKIDPNAFKISSDGSGGVLIDSGMTYTKLANGGFELLYDEIVDLMKGLLERIPTQ
Sbjct: 690 VGSKTLKIDPNAFKISSDGSGGVLIDSGMTYTKLANGGFELLYDEIVDLMKGLLERIPTQ 749

Query: 361 RKFEGLCFKGVVSRDLVGFPAVTFHFAGGADLVLESGSLFRQHGGDRFCLAILPSNSELL 420
           RKFEGLCFKGVVSRDLVGFPAVTFHFAGGADLVLESGSLFRQHGGDRFCLAILPSNSELL
Sbjct: 750 RKFEGLCFKGVVSRDLVGFPAVTFHFAGGADLVLESGSLFRQHGGDRFCLAILPSNSELL 809

Query: 421 NLSVIGILAQQNYNVGFDLEQMKVFFRRIDCQLLDE 457
           NLSVIGILAQQNYNVGFDLEQMKVFFRRIDCQLLDE
Sbjct: 810 NLSVIGILAQQNYNVGFDLEQMKVFFRRIDCQLLDE 844

[Close]
BLAST of PI0027280 vs. NCBI nr
Match: TYK28585.1 (Peptidase A1 [Cucumis melo var. makuwa])

HSP 1 Score: 892.5 bits (2305), Expect = 1.5e-255
Identity = 443/456 (97.15%), Postives = 448/456 (98.25%), Query Frame = 0 

Query: 1   MMILLTSLHHLLPSLTLAFYLSTAVISSASITTKPSRLATKLIHRNSYLHPLYDPNETVE 60
           MMILL SLHHLLPSLTLAFYLSTA+I S SI TKPSRLATKLIHRNSYLHPLYDPNETVE
Sbjct: 390 MMILLASLHHLLPSLTLAFYLSTAIILSTSIMTKPSRLATKLIHRNSYLHPLYDPNETVE 449

Query: 61  DQSKREQTSSIERFAYLESKIKELKSVGNEARSSLIPFNRGSGFLVNLSIGSPPVTQLVV 120
           D+SKREQ SSIERFA+LESKIKELKSVGNEARSSLIPFNRGSGFLVNLSIGSPPVTQLVV
Sbjct: 450 DRSKREQASSIERFAFLESKIKELKSVGNEARSSLIPFNRGSGFLVNLSIGSPPVTQLVV 509

Query: 121 VDTGSSLLWVQCLPCINCFQQSSSWFDPLKSASFKTLGCGFPGYKYINGYKCNGFNQAEY 180
           VDTGSSLLWVQCLPCINCFQQS+SWFDPLKSASFKTLGCGFPGY YINGYKCNG NQAEY
Sbjct: 510 VDTGSSLLWVQCLPCINCFQQSTSWFDPLKSASFKTLGCGFPGYNYINGYKCNG-NQAEY 569

Query: 181 KLRYLGGDSSQGILAKESLLFETLDEGKIKKTNLTFGCGHMNFKTNNDDTYNGVFGLGAY 240
           KLRYLGGDSSQGILAKESLLFETLDEGKIKKTNLTFGCGHMNFKTNNDDTYNGVFGLGAY
Sbjct: 570 KLRYLGGDSSQGILAKESLLFETLDEGKIKKTNLTFGCGHMNFKTNNDDTYNGVFGLGAY 629

Query: 241 PHITMATQLGNKFSYCIGDINNPLYTHNHLVLGQGSYIEGDFTPLQIHFGHYYVTLQSIS 300
           PHITMATQLGNKFSYCIGDINNPLYTHNHLVLGQGSYIEGD TPLQIHFGHYYVTLQ+IS
Sbjct: 630 PHITMATQLGNKFSYCIGDINNPLYTHNHLVLGQGSYIEGDSTPLQIHFGHYYVTLQTIS 689

Query: 301 VGSKTLKIDPNAFKISSDGSGGVLIDSGMTYTKLANGGFELLYDEIVDLMKGLLERIPTQ 360
           VGSKTLKIDPNAFKISSDGSGGVLIDSGMTYTKLANGGFELLYDEIVDLMKGLLERIPTQ
Sbjct: 690 VGSKTLKIDPNAFKISSDGSGGVLIDSGMTYTKLANGGFELLYDEIVDLMKGLLERIPTQ 749

Query: 361 RKFEGLCFKGVVSRDLVGFPAVTFHFAGGADLVLESGSLFRQHGGDRFCLAILPSNSELL 420
           RKFEGLCFKGVVSRDLVGFPAVTFHFAGGADLVLESGSLFRQHGGDRFCLAILPSNSELL
Sbjct: 750 RKFEGLCFKGVVSRDLVGFPAVTFHFAGGADLVLESGSLFRQHGGDRFCLAILPSNSELL 809

Query: 421 NLSVIGILAQQNYNVGFDLEQMKVFFRRIDCQLLDE 457
           NLSVIGILAQQNYNVGFDLEQMKVFFRRIDCQLLDE
Sbjct: 810 NLSVIGILAQQNYNVGFDLEQMKVFFRRIDCQLLDE 844

[Close]
BLAST of PI0027280 vs. NCBI nr
Match: XP_031742409.1 (aspartic proteinase CDR1 [Cucumis sativus] >KGN65478.1 hypothetical protein Csa_020065 [Cucumis sativus])

HSP 1 Score: 888.3 bits (2294), Expect = 2.9e-254
Identity = 439/456 (96.27%), Postives = 445/456 (97.59%), Query Frame = 0 

Query: 1   MMILLTSLHHLLPSLTLAFYLSTAVISSASITTKPSRLATKLIHRNSYLHPLYDPNETVE 60
           MMILL SLHHLLPSLTLAFYLSTA+ISS  ITTKPSRLATKLIHRNSYLHPLYD NETVE
Sbjct: 1   MMILLASLHHLLPSLTLAFYLSTAIISSTLITTKPSRLATKLIHRNSYLHPLYDQNETVE 60

Query: 61  DQSKREQTSSIERFAYLESKIKELKSVGNEARSSLIPFNRGSGFLVNLSIGSPPVTQLVV 120
           D+SKREQTSSIERF +LESKIKELKSVGNEARSSLIPFNRGSGFLVNLSIGSPPVTQLVV
Sbjct: 61  DRSKREQTSSIERFDFLESKIKELKSVGNEARSSLIPFNRGSGFLVNLSIGSPPVTQLVV 120

Query: 121 VDTGSSLLWVQCLPCINCFQQSSSWFDPLKSASFKTLGCGFPGYKYINGYKCNGFNQAEY 180
           VDTGSSLLWVQCLPCINCFQQS+SWFDPLKS SFKTLGCGFPGY YINGYKCN FNQAEY
Sbjct: 121 VDTGSSLLWVQCLPCINCFQQSTSWFDPLKSVSFKTLGCGFPGYNYINGYKCNRFNQAEY 180

Query: 181 KLRYLGGDSSQGILAKESLLFETLDEGKIKKTNLTFGCGHMNFKTNNDDTYNGVFGLGAY 240
           KLRYLGGDSSQGILAKESLLFETLDEGKIKK+N+TFGCGHMN KTNNDD YNGVFGLGAY
Sbjct: 181 KLRYLGGDSSQGILAKESLLFETLDEGKIKKSNITFGCGHMNIKTNNDDAYNGVFGLGAY 240

Query: 241 PHITMATQLGNKFSYCIGDINNPLYTHNHLVLGQGSYIEGDFTPLQIHFGHYYVTLQSIS 300
           PHITMATQLGNKFSYCIGDINNPLYTHNHLVLGQGSYIEGD TPLQIHFGHYYVTLQSIS
Sbjct: 241 PHITMATQLGNKFSYCIGDINNPLYTHNHLVLGQGSYIEGDSTPLQIHFGHYYVTLQSIS 300

Query: 301 VGSKTLKIDPNAFKISSDGSGGVLIDSGMTYTKLANGGFELLYDEIVDLMKGLLERIPTQ 360
           VGSKTLKIDPNAFKISSDGSGGVLIDSGMTYTKLANGGFELLYDEIVDLMKGLLERIPTQ
Sbjct: 301 VGSKTLKIDPNAFKISSDGSGGVLIDSGMTYTKLANGGFELLYDEIVDLMKGLLERIPTQ 360

Query: 361 RKFEGLCFKGVVSRDLVGFPAVTFHFAGGADLVLESGSLFRQHGGDRFCLAILPSNSELL 420
           RKFEGLCFKGVVSRDLVGFPAVTFHFAGGADLVLESGSLFRQHGGDRFCLAILPSNSELL
Sbjct: 361 RKFEGLCFKGVVSRDLVGFPAVTFHFAGGADLVLESGSLFRQHGGDRFCLAILPSNSELL 420

Query: 421 NLSVIGILAQQNYNVGFDLEQMKVFFRRIDCQLLDE 457
           NLSVIGILAQQNYNVGFDLEQMKVFFRRIDCQLLDE
Sbjct: 421 NLSVIGILAQQNYNVGFDLEQMKVFFRRIDCQLLDE 456

[Close]
BLAST of PI0027280 vs. NCBI nr
Match: XP_008453701.1 (PREDICTED: aspartic proteinase CDR1-like [Cucumis melo])

HSP 1 Score: 879.0 bits (2270), Expect = 1.7e-251
Identity = 441/472 (93.43%), Postives = 448/472 (94.92%), Query Frame = 0 

Query: 1   MMILLTSLHHLLPSLTLAFYLSTAVISSASITTKPSRLATKLIHRNSYLHPLYDPNETVE 60
           MMILL SLHHLLPSLTLAFYLSTA+I S SITTKPSRLATKLIHRNSYLHPLYDPNETVE
Sbjct: 14  MMILLASLHHLLPSLTLAFYLSTAIILSTSITTKPSRLATKLIHRNSYLHPLYDPNETVE 73

Query: 61  DQSKREQTSSIERFAYLESKIKELKSVGNEARSSLIPFNRGSGFLVNLSIGSPPVTQLVV 120
           D+SKREQ SSIERFA+LESKIKELKSVGNEARSSLIPFNRGSGFLVNLSIGSPPVTQLVV
Sbjct: 74  DRSKREQASSIERFAFLESKIKELKSVGNEARSSLIPFNRGSGFLVNLSIGSPPVTQLVV 133

Query: 121 VDTGSSLLWVQCLPCINCFQQSSSWFDPLKSASFKTLGCGFPGYKYINGYKCNGFNQAEY 180
           VDTGSSLLWVQCLPCINCFQQS+SWFDPLKSASFKTLGCGFPGY YINGYKCNG NQAEY
Sbjct: 134 VDTGSSLLWVQCLPCINCFQQSTSWFDPLKSASFKTLGCGFPGYNYINGYKCNG-NQAEY 193

Query: 181 KLRYLGGDSSQGILAKESLLFETLDEGKI----------------KKTNLTFGCGHMNFK 240
           KLRYLGGDSSQGILAKESLLFETLDEG +                KKTNLTFGCGHMNFK
Sbjct: 194 KLRYLGGDSSQGILAKESLLFETLDEGGVFQYNALFQHKQVNKQNKKTNLTFGCGHMNFK 253

Query: 241 TNNDDTYNGVFGLGAYPHITMATQLGNKFSYCIGDINNPLYTHNHLVLGQGSYIEGDFTP 300
           TNNDDTYNGVFGLGAYP+ITMATQLGNKFSYCIGDINNPLYTHNHLVLGQGSYIEGD TP
Sbjct: 254 TNNDDTYNGVFGLGAYPYITMATQLGNKFSYCIGDINNPLYTHNHLVLGQGSYIEGDSTP 313

Query: 301 LQIHFGHYYVTLQSISVGSKTLKIDPNAFKISSDGSGGVLIDSGMTYTKLANGGFELLYD 360
           LQIHFGHYYVTLQ+ISVGSKTLKIDPNAFKISSDGSGGVLIDSGMTYTKLANGGFELLYD
Sbjct: 314 LQIHFGHYYVTLQTISVGSKTLKIDPNAFKISSDGSGGVLIDSGMTYTKLANGGFELLYD 373

Query: 361 EIVDLMKGLLERIPTQRKFEGLCFKGVVSRDLVGFPAVTFHFAGGADLVLESGSLFRQHG 420
           EIVDLMKGLLERIPTQRKFEGLCFKGVVSRDLVGFPAVTFHFAGGADLVLESGSLFRQHG
Sbjct: 374 EIVDLMKGLLERIPTQRKFEGLCFKGVVSRDLVGFPAVTFHFAGGADLVLESGSLFRQHG 433

Query: 421 GDRFCLAILPSNSELLNLSVIGILAQQNYNVGFDLEQMKVFFRRIDCQLLDE 457
           GDRFCLAILPSNSELLNLSVIGILAQQNYNVGFDLEQMKVFFRRIDCQLLDE
Sbjct: 434 GDRFCLAILPSNSELLNLSVIGILAQQNYNVGFDLEQMKVFFRRIDCQLLDE 484

[Close]
BLAST of PI0027280 vs. NCBI nr
Match: XP_038878960.1 (aspartic proteinase CDR1-like isoform X1 [Benincasa hispida])

HSP 1 Score: 845.5 bits (2183), Expect = 2.1e-241
Identity = 411/456 (90.13%), Postives = 436/456 (95.61%), Query Frame = 0 

Query: 1   MMILLTSLHHLLPSLTLAFYLSTAVISSASITTKPSRLATKLIHRNSYLHPLYDPNETVE 60
           M+ILL S +HLLPSLTLAFYLSTA+ISS + TTKPSRLATKLIHRNSYLHPLYDP ET+E
Sbjct: 1   MVILLVSQYHLLPSLTLAFYLSTAIISSMAFTTKPSRLATKLIHRNSYLHPLYDPTETIE 60

Query: 61  DQSKREQTSSIERFAYLESKIKELKSVGNEARSSLIPFNRGSGFLVNLSIGSPPVTQLVV 120
           D+SKRE+TSSIERFAYLESKIKELKSVGNEARSSLIPFN+GSGFLVNLSIGSPPVTQLVV
Sbjct: 61  DRSKREETSSIERFAYLESKIKELKSVGNEARSSLIPFNQGSGFLVNLSIGSPPVTQLVV 120

Query: 121 VDTGSSLLWVQCLPCINCFQQSSSWFDPLKSASFKTLGCGFPGYKYINGYKCNGFNQAEY 180
            DTGSSLLWVQCLPCI+CF+QS+SWFDPLKS SFK LGCGF GY YI+GY+CNGFNQAEY
Sbjct: 121 ADTGSSLLWVQCLPCIDCFRQSNSWFDPLKSTSFKILGCGFAGYNYISGYRCNGFNQAEY 180

Query: 181 KLRYLGGDSSQGILAKESLLFETLDEGKIKKTNLTFGCGHMNFKTNNDDTYNGVFGLGAY 240
           KLRYLGGD+SQG+LAKESLLFETLDEGKIKKTNLTFGCGHMN KTNNDDTYNGVFGLGAY
Sbjct: 181 KLRYLGGDTSQGVLAKESLLFETLDEGKIKKTNLTFGCGHMNSKTNNDDTYNGVFGLGAY 240

Query: 241 PHITMATQLGNKFSYCIGDINNPLYTHNHLVLGQGSYIEGDFTPLQIHFGHYYVTLQSIS 300
           P+ITMATQLGNKFSYCIGDINNP+YTHNHLVLG+GSYIEGD TPLQIHFGHYYVTLQ IS
Sbjct: 241 PYITMATQLGNKFSYCIGDINNPVYTHNHLVLGEGSYIEGDSTPLQIHFGHYYVTLQGIS 300

Query: 301 VGSKTLKIDPNAFKISSDGSGGVLIDSGMTYTKLANGGFELLYDEIVDLMKGLLERIPTQ 360
           VGSK LKIDP AF+++ DG GGVLIDSGMTYTKLANGGFELLYDEI+DLM GLLERIPT+
Sbjct: 301 VGSKRLKIDPKAFQMTWDGRGGVLIDSGMTYTKLANGGFELLYDEILDLMTGLLERIPTE 360

Query: 361 RKFEGLCFKGVVSRDLVGFPAVTFHFAGGADLVLESGSLFRQHGGDRFCLAILPSNSELL 420
           RKFEGLCFKGVVSRDL+GFP VTFHFAGGADLVLESGSLFRQHGGDRFCLAILPSNSE+L
Sbjct: 361 RKFEGLCFKGVVSRDLIGFPTVTFHFAGGADLVLESGSLFRQHGGDRFCLAILPSNSEML 420

Query: 421 NLSVIGILAQQNYNVGFDLEQMKVFFRRIDCQLLDE 457
           NLSVIGILAQQNYNV FDLEQMKVFFRRIDCQLLDE
Sbjct: 421 NLSVIGILAQQNYNVAFDLEQMKVFFRRIDCQLLDE 456

[Close]
BLAST of PI0027280 vs. TAIR 10
Match: AT4G30030.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 308.5 bits (789), Expect = 8.8e-84
Identity = 166/393 (42.24%), Postives = 234/393 (59.54%), Query Frame = 0 

Query: 65  REQTSSIERFAYLESKIKELKSVGN-EARSSLIPFNRGSGFLVNLSIGSPPVTQLVVVDT 124
           + Q SS  +  YL SK      + N    S + P    + FL N+SIG+PPV QL+++DT
Sbjct: 38  KTQESSKIKIGYLHSKSTPASRLDNLWTVSHVTPIPNPAAFLANISIGNPPVPQLLLIDT 97

Query: 125 GSSLLWVQCLPCINCFQQSSSWFDPLKSASFKTLGCGFPGYKYINGYKCNGFNQAEYKLR 184
           GS L W+ CLPC  C+ Q+  +F P +S++++   C    +     ++       +Y LR
Sbjct: 98  GSDLTWIHCLPC-KCYPQTIPFFHPSRSSTYRNASCVSAPHAMPQIFRDEKTGNCQYHLR 157

Query: 185 YLGGDSSQGILAKESLLFETLDEGKIKKTNLTFGCGHMNFKTNNDDTYNGVFGLGAYPHI 244
           Y    +++GILA+E L FET D+G I K N+ FGCG  N   +    Y+GV GLG     
Sbjct: 158 YRDFSNTRGILAEEKLTFETSDDGLISKQNIVFGCGQDN---SGFTKYSGVLGLGPGTFS 217

Query: 245 TMATQLGNKFSYCIGDINNPLYTHNHLVLGQGSYIEGDFTPLQIHFGHYYVTLQSISVGS 304
            +    G+KFSYC G + NP Y HN L+LG G+ IEGD TPLQI    YY+ LQ+IS G 
Sbjct: 218 IVTRNFGSKFSYCFGSLTNPTYPHNILILGNGAKIEGDPTPLQIFQDRYYLDLQAISFGE 277

Query: 305 KTLKIDPNAFKISSDGSGGVLIDSGMTYTKLANGGFELLYDEIVDLMKGLLERIPTQRKF 364
           K L I+P  F+      GG +ID+G + T LA   +E L +EI  L+  +L R+    ++
Sbjct: 278 KLLDIEPGTFQ-RYRSQGGTVIDTGCSPTILAREAYETLSEEIDFLLGEVLRRVKDWDQY 337

Query: 365 EGLCFKGVVSRDLVGFPAVTFHFAGGADLVLESGSLF-RQHGGDRFCLAILPSNSELLNL 424
              C++G +  DL GFP VTFHFAGGA+L L+  SLF     GD FCLA+  +  +  ++
Sbjct: 338 TTPCYEGNLKLDLYGFPVVTFHFAGGAELALDVESLFVSSESGDSFCLAMTMNTFD--DM 397

Query: 425 SVIGILAQQNYNVGFDLEQMKVFFRRIDCQLLD 456
           SVIG +AQQNYNVG++L  MKV+F+R DC+++D
Sbjct: 398 SVIGAMAQQNYNVGYNLRTMKVYFQRTDCEIID 423

[Close]
BLAST of PI0027280 vs. TAIR 10
Match: AT2G23945.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 291.2 bits (744), Expect = 1.4e-78
Identity = 181/460 (39.35%), Postives = 265/460 (57.61%), Query Frame = 0 

Query: 11  LLPSLTLAFYLSTAVISSASITTKPSRLATKLIHRNSY--LHPLYDPNETVEDQSKREQT 70
           LL  +T+++++ T  I       KP+R+A KLIHR S   L+P      T ED  K    
Sbjct: 9   LLLFITVSYFVVTESI-------KPNRMAMKLIHRESVARLNPNARVPITPEDHIKHLTD 68

Query: 71  SSIERFAYLESKI-KELKSVGNEARSSLIPFNRGSGFLVNLSIGSPPVTQLVVVDTGSSL 130
            S  RF YL++ I KEL S  +  +  +    + S FLVN S+G PPV QL ++DTGSSL
Sbjct: 69  ISSARFKYLQNSIDKELGS--SNFQVDVEQAIKTSLFLVNFSVGQPPVPQLTIMDTGSSL 128

Query: 131 LWVQCLPCINCFQQS--SSWFDPLKSASFKTLGCGFPGYKYINGYKCNGFNQAEYKLRYL 190
           LW+QC PC +C         F+P  S++F    C     +Y     C   N+  Y+  Y+
Sbjct: 129 LWIQCQPCKHCSSDHMIHPVFNPALSSTFVECSCDDRFCRYAPNGHCGSSNKCVYEQVYI 188

Query: 191 GGDSSQGILAKESLLFETLDEGKIKKTNLTFGCGHMNFKTNNDDTYNGVFGLGAYPHITM 250
            G  S+G+LAKE L F T +   +    + FGCG+ N +   +  + G+ GLGA P  ++
Sbjct: 189 SGTGSKGVLAKERLTFTTPNGNTVVTQPIAFGCGYENGE-QLESHFTGILGLGAKP-TSL 248

Query: 251 ATQLGNKFSYCIGDINNPLYTHNHLVLGQGSYIEGDFTPLQIHFGH--YYVTLQSISVGS 310
           A QLG+KFSYCIGD+ N  Y +N LVLG+ + I GD TP++    +  YY+ L+ ISVG 
Sbjct: 249 AVQLGSKFSYCIGDLANKNYGYNQLVLGEDADILGDPTPIEFETENSIYYMNLEGISVGD 308

Query: 311 KTLKIDPNAFKISSDGSGGVLIDSGMTYTKLANGGFELLYDEIVDLMKGLLERIPTQRKF 370
             L I+P  FK     + GV++DSG  YT LA+  +  LY+EI  ++   LER   +   
Sbjct: 309 TQLNIEPVVFKRRGPRT-GVILDSGTLYTWLADIAYRELYNEIKSILDPKLERFWFR--- 368

Query: 371 EGLCFKGVVSRDLVGFPAVTFHFAGGADLVLESGSLF----RQHGGDRFCLAILPS---N 430
           + LC+ G VS +L+GFP VTFHFAGGA+L +E+ S+F      +  + FC+++ P+    
Sbjct: 369 DFLCYHGRVSEELIGFPVVTFHFAGGAELAMEATSMFYPLSEPNTFNVFCMSVKPTKEHG 428

Query: 431 SELLNLSVIGILAQQNYNVGFDLEQMKVFFRRIDCQLLDE 457
            E    + IG++AQQ YN+G+DL++  ++ +RIDC  LD+
Sbjct: 429 GEYKEFTAIGLMAQQYYNIGYDLKEKNIYLQRIDCVQLDD 453

[Close]
BLAST of PI0027280 vs. TAIR 10
Match: AT4G30040.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 271.6 bits (693), Expect = 1.2e-72
Identity = 164/389 (42.16%), Postives = 224/389 (57.58%), Query Frame = 0 

Query: 69  SSIERFAYLESKIKELKSVGNEARSSLIPFNRGSGFLVNLSIGSPPVTQLVVVDTGSSLL 128
           +S+ER  YL++K              +IP      FLVN+SIGSPP+TQL+ +DT S LL
Sbjct: 54  ASVERLEYLKAKTTGDIIAHLSPNVPIIP----QAFLVNISIGSPPITQLLHMDTASDLL 113

Query: 129 WVQCLPCINCFQQSSSWFDPLKSASFKTLGCGFPGYKYINGYKCNGFNQA-EYKLRYLGG 188
           W+QCLPCINC+ QS   FDP +S + +   C    Y  +   K N   ++ EY +RY+  
Sbjct: 114 WIQCLPCINCYAQSLPIFDPSRSYTHRNETCRTSQYS-MPSLKFNANTRSCEYSMRYVDD 173

Query: 189 DSSQGILAKESLLFETL--DEGKIKKTNLTFGCGHMNFKTNNDDTYNGVFGLGAYPHITM 248
             S+GILA+E LLF T+  +       ++ FGCGH N+      T  G+ GLG Y   ++
Sbjct: 174 TGSKGILAREMLLFNTIYDESSSAALHDVVFGCGHDNYGEPLVGT--GILGLG-YGEFSL 233

Query: 249 ATQLGNKFSYCIGDINNPLYTHNHLVLG-QGSYIEGDFTPLQIHFGHYYVTLQSISVGSK 308
             + G KFSYC G +++P Y HN LVLG  G+ I GD TPL+IH G YYVT+++ISV   
Sbjct: 234 VHRFGKKFSYCFGSLDDPSYPHNVLVLGDDGANILGDTTPLEIHNGFYYVTIEAISVDGI 293

Query: 309 TLKIDPNAF-KISSDGSGGVLIDSGMTYTKLANGGFELLYDEIVDLMKGLLERIPTQR-- 368
            L IDP  F +    G GG +ID+G + T L    ++ L + I D+ +G        +  
Sbjct: 294 ILPIDPRVFNRNHQTGLGGTIIDTGNSLTSLVEEAYKPLKNRIEDIFEGRFTAADVSQDD 353

Query: 369 --KFEGLCFKGVVSRDLV--GFPAVTFHFAGGADLVLESGSLFRQHGGDRFCLAILPSNS 428
             K E  C+ G   RDLV  GFP VTFHF+ GA+L L+  SLF +   + FCLA+ P   
Sbjct: 354 MIKME--CYNGNFERDLVESGFPIVTFHFSEGAELSLDVKSLFMKLSPNVFCLAVTPG-- 413

Query: 429 ELLNLSVIGILAQQNYNVGFDLEQMKVFF 447
              NL+ IG  AQQ+YN+G+DLE M+V F
Sbjct: 414 ---NLNSIGATAQQSYNIGYDLEAMEVSF 427

[Close]
BLAST of PI0027280 vs. TAIR 10
Match: AT2G35615.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 188.3 bits (477), Expect = 1.3e-47
Identity = 142/461 (30.80%), Postives = 234/461 (50.76%), Query Frame = 0 

Query: 15  LTLAFYLSTAVISSASITTKPSRLATKLIHRNSYLHPLYDPNETVEDQSKREQTSSIERF 74
           + L F+L  +V  S+S    P   + +LIHR+S L P+Y+P  TV D+       S+ R 
Sbjct: 5   ILLCFFLFFSVTLSSS--GHPKNFSVELIHRDSPLSPIYNPQITVTDRLNAAFLRSVSRS 64

Query: 75  AYLESKIKELKSVGNEARSSLIPFNRGSGFLVNLSIGSPPVTQLVVVDTGSSLLWVQCLP 134
                ++ +      + +S LI       F ++++IG+PP+    + DTGS L WVQC P
Sbjct: 65  RRFNHQLSQ-----TDLQSGLI--GADGEFFMSITIGTPPIKVFAIADTGSDLTWVQCKP 124

Query: 135 CINCFQQSSSWFDPLKSASFKTLGCGFPGYKYINGYK--CNGFNQAEYKLRYLGGDS--S 194
           C  C++++   FD  KS+++K+  C     + ++  +  C+  N    K RY  GD   S
Sbjct: 125 CQQCYKENGPIFDKKKSSTYKSEPCDSRNCQALSSTERGCDESNNI-CKYRYSYGDQSFS 184

Query: 195 QGILAKESLLFETLDEGKIKKTNLTFGCGHMNFKTNNDDTYNGVFGLGAYPHITMATQLG 254
           +G +A E++  ++     +      FGCG+ N  T  D+T +G+ GLG   H+++ +QLG
Sbjct: 185 KGDVATETVSIDSASGSPVSFPGTVFGCGYNNGGT-FDETGSGIIGLGG-GHLSLISQLG 244

Query: 255 N----KFSYCIGDINNPLYTHNHLVLGQG-----SYIEGD----FTPL--QIHFGHYYVT 314
           +    KFSYC+   +    T+   V+  G     S +  D     TPL  +    +YY+T
Sbjct: 245 SSISKKFSYCLS--HKSATTNGTSVINLGTNSIPSSLSKDSGVVSTPLVDKEPLTYYYLT 304

Query: 315 LQSISVGSKTL-----KIDPNAFKISSDGSGGVLIDSGMTYTKLANGGFELLYDEIVDLM 374
           L++ISVG K +       +PN   I S+ SG ++IDSG T T L  G F+     + + +
Sbjct: 305 LEAISVGKKKIPYTGSSYNPNDDGILSETSGNIIIDSGTTLTLLEAGFFDKFSSAVEESV 364

Query: 375 KGLLERIPTQRKFEGLCFKGVVSRDLVGFPAVTFHFAGGADLVLESGSLFRQHGGDRFCL 434
            G  +R+   +     CFK   +   +G P +T HF  GAD+ L   + F +   D  CL
Sbjct: 365 TG-AKRVSDPQGLLSHCFKSGSAE--IGLPEITVHFT-GADVRLSPINAFVKLSEDMVCL 424

Query: 435 AILPSNSELLNLSVIGILAQQNYNVGFDLEQMKVFFRRIDC 452
           +++P+      +++ G  AQ ++ VG+DLE   V F+ +DC
Sbjct: 425 SMVPTT----EVAIYGNFAQMDFLVGYDLETRTVSFQHMDC 443

[Close]
BLAST of PI0027280 vs. TAIR 10
Match: AT1G31450.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 179.5 bits (454), Expect = 6.1e-45
Identity = 132/451 (29.27%), Postives = 229/451 (50.78%), Query Frame = 0 

Query: 21  LSTAVISSASITTKPSRLATKLIHRNSYLHPLYDPNETVEDQSKREQTSSIERFAYLESK 80
           L+ +   +++ +     L  +LIHR+S   PLY+P+ TV D+       SI R     +K
Sbjct: 12  LAISFFFASNSSANRENLTVELIHRDSPHSPLYNPHHTVSDRLNAAFLRSISRSRRFTTK 71

Query: 81  IKELKSVGNEARSSLIPFNRGSGFLVNLSIGSPPVTQLVVVDTGSSLLWVQCLPCINCFQ 140
                    + +S LI  + G  + +++SIG+PP     + DTGS L WVQC PC  C++
Sbjct: 72  --------TDLQSGLI--SNGGEYFMSISIGTPPSKVFAIADTGSDLTWVQCKPCQQCYK 131

Query: 141 QSSSWFDPLKSASFKTLGCGFPGYKYINGYKCNGFNQAE--YKLRYLGGDSS--QGILAK 200
           Q+S  FD  KS+++KT  C     + ++ ++  G ++++   K RY  GD+S  +G +A 
Sbjct: 132 QNSPLFDKKKSSTYKTESCDSKTCQALSEHE-EGCDESKDICKYRYSYGDNSFTKGDVAT 191

Query: 201 ESLLFETLDEGKIKKTNLTFGCGHMNFKTNNDDTYNGVFGLGAYPHITMATQLGN----K 260
           E++  ++     +      FGCG+ N  T  ++T +G+ GLG  P +++ +QLG+    K
Sbjct: 192 ETISIDSSSGSSVSFPGTVFGCGYNNGGT-FEETGSGIIGLGGGP-LSLVSQLGSSIGKK 251

Query: 261 FSYCIGDINNPLYTHNHLVLGQGSYIEGD-------FTPL--QIHFGHYYVTLQSISVGS 320
           FSYC+          + + LG  S             TPL  +    +Y++TL++++VG 
Sbjct: 252 FSYCLSHTAATTNGTSVINLGTNSIPSNPSKDSATLTTPLIQKDPETYYFLTLEAVTVGK 311

Query: 321 KTLKIDPNAFKI---SSDGSGGVLIDSGMTYTKLANGGFELLYDEIVDLMKGLLERIPTQ 380
             L      + +   SS  +G ++IDSG T T L +G ++     + + + G  +R+   
Sbjct: 312 TKLPYTGGGYGLNGKSSKRTGNIIIDSGTTLTLLDSGFYDDFGTAVEESVTG-AKRVSDP 371

Query: 381 RKFEGLCFKGVVSRDLVGFPAVTFHFAGGADLVLESGSLFRQHGGDRFCLAILPSNSELL 440
           +     CFK       +G PA+T HF   AD+ L   + F +   D  CL+++P+     
Sbjct: 372 QGLLTHCFKS--GDKEIGLPAITMHFT-NADVKLSPINAFVKLNEDTVCLSMIPTT---- 431

Query: 441 NLSVIGILAQQNYNVGFDLEQMKVFFRRIDC 452
            +++ G + Q ++ VG+DLE   V F+R+DC
Sbjct: 432 EVAIYGNMVQMDFLVGYDLETKTVSFQRMDC 441

The following BLAST results are available for this feature:
BLAST of PI0027280 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q3EBM51.9e-4630.80Probable aspartic protease At2g35615 OS=Arabidopsis thaliana OX=3702 GN=At2g3561... [more]
Q6XBF88.6e-4430.70Aspartic proteinase CDR1 OS=Arabidopsis thaliana OX=3702 GN=CDR1 PE=1 SV=1[more]
Q766C38.1e-4234.08Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 S... [more]
Q766C26.4e-3929.25Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 S... [more]
Q9SV771.0e-3631.11Aspartyl protease UND OS=Arabidopsis thaliana OX=3702 GN=UND PE=2 SV=1[more]
BLAST of PI0027280 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A5D3DZ207.4e-25697.15Peptidase A1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold629G002350 ... [more]
A0A5A7UU117.4e-25697.15Aspartic proteinase CDR1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_sc... [more]
A0A0A0LUP51.4e-25496.27Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G42435... [more]
A0A1S3BY288.4e-25293.43aspartic proteinase CDR1-like OS=Cucumis melo OX=3656 GN=LOC103494349 PE=3 SV=1[more]
A0A6J1C0L11.0e-22584.03probable aspartic protease At2g35615 OS=Momordica charantia OX=3673 GN=LOC111006... [more]
BLAST of PI0027280 vs. NCBI nr
Match NameE-valueIdentityDescription
KAA0058227.11.5e-25597.15aspartic proteinase CDR1-like [Cucumis melo var. makuwa][more]
TYK28585.11.5e-25597.15Peptidase A1 [Cucumis melo var. makuwa][more]
XP_031742409.12.9e-25496.27aspartic proteinase CDR1 [Cucumis sativus] >KGN65478.1 hypothetical protein Csa_... [more]
XP_008453701.11.7e-25193.43PREDICTED: aspartic proteinase CDR1-like [Cucumis melo][more]
XP_038878960.12.1e-24190.13aspartic proteinase CDR1-like isoform X1 [Benincasa hispida][more]
BLAST of PI0027280 vs. TAIR 10
Match NameE-valueIdentityDescription
AT4G30030.18.8e-8442.24Eukaryotic aspartyl protease family protein [more]
AT2G23945.11.4e-7839.35Eukaryotic aspartyl protease family protein [more]
AT4G30040.11.2e-7242.16Eukaryotic aspartyl protease family protein [more]
AT2G35615.11.3e-4730.80Eukaryotic aspartyl protease family protein [more]
AT1G31450.16.1e-4529.27Eukaryotic aspartyl protease family protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR032861Xylanase inhibitor, N-terminalPFAMPF14543TAXi_Ncoord: 104..272
e-value: 3.6E-33
score: 115.3
IPR021109Aspartic peptidase domain superfamilyGENE3D2.40.70.10Acid Proteasescoord: 86..273
e-value: 1.6E-35
score: 124.8
IPR021109Aspartic peptidase domain superfamilyGENE3D2.40.70.10Acid Proteasescoord: 278..454
e-value: 2.1E-41
score: 143.5
IPR021109Aspartic peptidase domain superfamilySUPERFAMILY50630Acid proteasescoord: 102..452
IPR032799Xylanase inhibitor, C-terminalPFAMPF14541TAXi_Ccoord: 291..447
e-value: 6.1E-30
score: 104.1
NoneNo IPR availablePANTHERPTHR47967OS07G0603500 PROTEIN-RELATEDcoord: 27..453
NoneNo IPR availablePANTHERPTHR47967:SF14EUKARYOTIC ASPARTYL PROTEASE FAMILY PROTEINcoord: 27..453
IPR001969Aspartic peptidase, active sitePROSITEPS00141ASP_PROTEASEcoord: 119..130
IPR033121Peptidase family A1 domainPROSITEPS51767PEPTIDASE_A1coord: 104..447
score: 32.38158
IPR034161Pepsin-like domain, plantCDDcd05476pepsin_A_like_plantcoord: 103..451
e-value: 1.04647E-69
score: 220.597

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0027280.1PI0027280.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0004190 aspartic-type endopeptidase activity
molecular_function GO:0016757 glycosyltransferase activity