PI0025052 (gene) Melon (PI 482460) v1

Overview


Name	PI0025052
Type	gene
Organism	Cucumis metuliferus (Melon (PI 482460) v1)
Description	Reverse transcriptase domain-containing protein
Location	chr05: 8561944 .. 8563215 (-)
RNA-Seq Expression	PI0025052
Synteny	PI0025052

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCCAACGCATCAAAGAGTGGCGAAGTTGTGGTCAGTGTTACAAATAACCGAAAATGTGTGCCGCTCATCCTGCGTATTACTAGTAGGATTCGGTCTTGGTCGACTAGAGTTTTGTTCTTCGCTGGGAGACTTCAGCTTGTTCGTTCGGTGTTGCAAAGCTTTCAGGTCTTTTGGGCTAGTGTGTTTGTTTTGCCAGCAAGTGTGCATCATGAGGTTGATAGGCTTTTGATGTCGTATCTCTGGAGGGGCAAGGAGATTGGGAGAGGGGGTGCGAAGGTTGCGTGGACTGAGGTGTGTGTACCATTTAATGAGGGGGCCTTAATATTCGTGAGGGGAAGTCGTGGAATATTGTGTGTATATTGAAGATTCTTTGGTTGATGTTGACTAATTCGGGGTCTTTGTGGGTGGCTTGGGTGGAGGCCTATGTTCTGAAGGGGAGGTTGTTGTGGTCTGTGAACTCTGAGATTGGTCGGTCCTGGTGTCTTCGTGCTATCCTGCGACAGCGTGATAGCCTAGGGTTGGTGATGGTTTGTTGTGGGGTGGGGGTAATATTCCCAGGCATTCTTTTTGTGCTTGGTTGGCCATCAGGGATAGGTTGGGCACTCGGGATAGGTTGAGTATGTGGGATAGGTCGATTCCTCAGTCGTGTCTTTTGTGCGAGGGGGGTCTTGAGTCTCGGGACCATCTGTTTTTTTCTTGTCCTTTTGGGTATGATATTTGGAATCATATCATTCGAGCTATGGCTTCCTGTCACAGGGTCGGGGGTGGGGAGTTGAGATGTATTAGGTCAGTCTTAGGGGTATTGGGAAGGGTGTGTTGAGGAGGCTATGACGTGTACTTTGGTGGTTTCTATCTACTTTATCTAGAATGAGCGAAATCGTTGTCTTAGTGTATCCGGTCTACGATTTGTGCTCGTGTTGTATCTTGGCCGGAGGATGTTCAGGATCTTATTTAGTGCTTTCCATTTTTGTTGTTTGTTGTCTCTTGTTACGGGTTGGTTTTTCTTTTTGGATTGTTTCCCCGATTGTGGGGTTGTTTTATCATTGGTTTCTTTTTATGTTTTCTTTGTTTTTGTAGTGTTTCGTGGTTTTTATGGTTATGTTTGTCCTTTTGAGCTTTTCTGGCTCTCGTGCCTTGGCCTCGGGCTGTGAAGACCTCCTTCTATTAATCGATGCTAGTTTGGATGGTCCAACTCGCACAGCATCCCAAAGTGTCGCGATGTTAGTAAGGCGATTTGGGGCGGGGTGTGACACAAAGACTTTAGTCTAA

mRNA sequence

ATGGCCAACGCATCAAAGAGTGGCGAAGTTGTGGTCAGTGTTACAAATAACCGAAAATGTGTGCCGCTCATCCTGCGTATTACTAGTAGGATTCGGTCTTGGTCGACTAGAGTTTTGTTCTTCGCTGGGAGACTTCAGCTTGTTCGTTCGGTGTTGCAAAGCTTTCAGGTCTTTTGGGCTAGTGTGTTTGTTTTGCCAGCAAGTGTGCATCATGAGGTTGATAGGCTTTTGATGTCGTATCTCTGGAGGGGCAAGGAGATTGGGAGAGGGGGTGCGAAGGTTGCGTGGACTGAGTGTTTCGTGGTTTTTATGGTTATGTTTGTCCTTTTGAGCTTTTCTGGCTCTCGTGCCTTGGCCTCGGGCTGTGAAGACCTCCTTCTATTAATCGATGCTAGTTTGGATGGTCCAACTCGCACAGCATCCCAAAGTGTCGCGATGTTAGTAAGGCGATTTGGGGCGGGGTGTGACACAAAGACTTTAGTCTAA

Coding sequence (CDS)

ATGGCCAACGCATCAAAGAGTGGCGAAGTTGTGGTCAGTGTTACAAATAACCGAAAATGTGTGCCGCTCATCCTGCGTATTACTAGTAGGATTCGGTCTTGGTCGACTAGAGTTTTGTTCTTCGCTGGGAGACTTCAGCTTGTTCGTTCGGTGTTGCAAAGCTTTCAGGTCTTTTGGGCTAGTGTGTTTGTTTTGCCAGCAAGTGTGCATCATGAGGTTGATAGGCTTTTGATGTCGTATCTCTGGAGGGGCAAGGAGATTGGGAGAGGGGGTGCGAAGGTTGCGTGGACTGAGTGTTTCGTGGTTTTTATGGTTATGTTTGTCCTTTTGAGCTTTTCTGGCTCTCGTGCCTTGGCCTCGGGCTGTGAAGACCTCCTTCTATTAATCGATGCTAGTTTGGATGGTCCAACTCGCACAGCATCCCAAAGTGTCGCGATGTTAGTAAGGCGATTTGGGGCGGGGTGTGACACAAAGACTTTAGTCTAA

Protein sequence

MANASKSGEVVVSVTNNRKCVPLILRITSRIRSWSTRVLFFAGRLQLVRSVLQSFQVFWASVFVLPASVHHEVDRLLMSYLWRGKEIGRGGAKVAWTECFVVFMVMFVLLSFSGSRALASGCEDLLLLIDASLDGPTRTASQSVAMLVRRFGAGCDTKTLV

Homology

[Close]

BLAST of PI0025052 vs. ExPASy Swiss-Prot
Match: P0C2F6 (Putative ribonuclease H protein At1g65750 OS=Arabidopsis thaliana OX=3702 GN=At1g65750 PE=3 SV=1)

HSP 1 Score: 51.6 bits (122), Expect = 9.6e-06
Identity = 25/76 (32.89%), Postives = 43/76 (56.58%), Query Frame = 0


Query: 23 LILRITSRIRSWSTRVLFFAGRLQLVRSVLQSFQVFWASVFVLPASVHHEVDRLLMSYLW 82
          ++ R++SR+  W  + L FAGRL L ++VL S  V   S  +LP S+ + +D+L  ++LW
Sbjct: 16 ILERVSSRMSGWREKTLSFAGRLTLTKAVLSSMPVHSMSTILLPQSILNRLDQLSRTFLW 75

Query: 83 RGKEIGRGGAKVAWTE 99
                +    V W++
Sbjct: 76 GSTAEKKKQHLVKWSK 91

[Close]

BLAST of PI0025052 vs. ExPASy TrEMBL
Match: A0A5A7TZS0 (Reverse transcriptase domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold19358G00020 PE=4 SV=1)

HSP 1 Score: 131.0 bits (328), Expect = 4.6e-27
Identity = 63/84 (75.00%), Postives = 71/84 (84.52%), Query Frame = 0


Query: 20  CVPLILRITSRIRSWSTRVLFFAGRLQLVRSVLQSFQVFWASVFVLPASVHHEVDRLLMS 79
           C PLI RITSRIRSW+ RVL FAGRLQLVRSVL+S QV+WASVFVLPA VH+EVD++L S
Sbjct: 734 CAPLIQRITSRIRSWTARVLSFAGRLQLVRSVLRSLQVYWASVFVLPAYVHNEVDKILRS 793

Query: 80  YLWRGKEIGRGGAKVAWTECFVVF 104
           YLWRGKE GRGG KVAW +  + F
Sbjct: 794 YLWRGKEEGRGGIKVAWVDVCLPF 817

[Close]

BLAST of PI0025052 vs. ExPASy TrEMBL
Match: A0A5A7UV01 (F17F8.5 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold896G00020 PE=4 SV=1)

HSP 1 Score: 129.8 bits (325), Expect = 1.0e-26
Identity = 69/111 (62.16%), Postives = 83/111 (74.77%), Query Frame = 0


Query: 22  PLILRITSRIRSWSTRVLFFAGRLQLVRSVLQSFQVFWASVFVLPASVHHEVDRLLMSYL 81
           PLI RITSRIRSW+ RVL FAGRLQLVR VL+S QV+WASVFVLPA VHHEVD++L+SYL
Sbjct: 426 PLIQRITSRIRSWTARVLSFAGRLQLVRFVLRSLQVYWASVFVLPAYVHHEVDKILISYL 485

Query: 82  WRGKEIGRGGAKVAWTECFVVFMVMFVLLSFSGSRALASGCEDLLLLIDAS 133
           WRGKE GRGG KVAW +  + F    + +    S  +AS  + LLL++  S
Sbjct: 486 WRGKEEGRGGIKVAWVDVCLPFEESGLGIRDGPSWNIASTLKILLLMLTNS 536

[Close]

BLAST of PI0025052 vs. ExPASy TrEMBL
Match: A0A5A7SPE5 (Reverse transcriptase domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold581G00620 PE=4 SV=1)

HSP 1 Score: 129.4 bits (324), Expect = 1.3e-26
Identity = 62/84 (73.81%), Postives = 71/84 (84.52%), Query Frame = 0


Query: 20   CVPLILRITSRIRSWSTRVLFFAGRLQLVRSVLQSFQVFWASVFVLPASVHHEVDRLLMS 79
            C PLI RITS+IRSW+ RVL FAGRLQLVRSVL+S QV+WASVFVLPA VH+EVD++L S
Sbjct: 1040 CAPLIQRITSQIRSWNARVLSFAGRLQLVRSVLRSLQVYWASVFVLPAYVHNEVDKILRS 1099

Query: 80   YLWRGKEIGRGGAKVAWTECFVVF 104
            YLWRGKE GRGG KVAW +  + F
Sbjct: 1100 YLWRGKEEGRGGIKVAWVDVCLPF 1123

[Close]

BLAST of PI0025052 vs. ExPASy TrEMBL
Match: A0A5D3DXE4 (Reverse transcriptase domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1467G00020 PE=4 SV=1)

HSP 1 Score: 129.4 bits (324), Expect = 1.3e-26
Identity = 62/84 (73.81%), Postives = 71/84 (84.52%), Query Frame = 0


Query: 20   CVPLILRITSRIRSWSTRVLFFAGRLQLVRSVLQSFQVFWASVFVLPASVHHEVDRLLMS 79
            C PLI RITS+IRSW+ RVL FAGRLQLVRSVL+S QV+WASVFVLPA VH+EVD++L S
Sbjct: 1040 CAPLIQRITSQIRSWNARVLSFAGRLQLVRSVLRSLQVYWASVFVLPAYVHNEVDKILRS 1099

Query: 80   YLWRGKEIGRGGAKVAWTECFVVF 104
            YLWRGKE GRGG KVAW +  + F
Sbjct: 1100 YLWRGKEEGRGGIKVAWVDVCLPF 1123

[Close]

BLAST of PI0025052 vs. ExPASy TrEMBL
Match: A0A5A7V3Z0 (Reverse transcriptase domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold154G00690 PE=4 SV=1)

HSP 1 Score: 127.9 bits (320), Expect = 3.9e-26
Identity = 62/82 (75.61%), Postives = 70/82 (85.37%), Query Frame = 0


Query: 22  PLILRITSRIRSWSTRVLFFAGRLQLVRSVLQSFQVFWASVFVLPASVHHEVDRLLMSYL 81
           PLI RITSRIRSW+ RVL FAGRLQLV SVL+SFQV+WASVFVLPA VH+EVD++L SYL
Sbjct: 532 PLIQRITSRIRSWTARVLSFAGRLQLVHSVLRSFQVYWASVFVLPAYVHNEVDKILRSYL 591

Query: 82  WRGKEIGRGGAKVAWTECFVVF 104
           WRGKE GRGG KVAW +  + F
Sbjct: 592 WRGKEEGRGGIKVAWVDVCLPF 613

[Close]

BLAST of PI0025052 vs. NCBI nr
Match: KAA0046851.1 (uncharacterized protein E6C27_scaffold19358G00020 [Cucumis melo var. makuwa])

HSP 1 Score: 131.0 bits (328), Expect = 9.5e-27
Identity = 63/84 (75.00%), Postives = 71/84 (84.52%), Query Frame = 0


Query: 20  CVPLILRITSRIRSWSTRVLFFAGRLQLVRSVLQSFQVFWASVFVLPASVHHEVDRLLMS 79
           C PLI RITSRIRSW+ RVL FAGRLQLVRSVL+S QV+WASVFVLPA VH+EVD++L S
Sbjct: 734 CAPLIQRITSRIRSWTARVLSFAGRLQLVRSVLRSLQVYWASVFVLPAYVHNEVDKILRS 793

Query: 80  YLWRGKEIGRGGAKVAWTECFVVF 104
           YLWRGKE GRGG KVAW +  + F
Sbjct: 794 YLWRGKEEGRGGIKVAWVDVCLPF 817

[Close]

BLAST of PI0025052 vs. NCBI nr
Match: KAA0059752.1 (F17F8.5 [Cucumis melo var. makuwa] >TYJ99767.1 F17F8.5 [Cucumis melo var. makuwa])

HSP 1 Score: 129.8 bits (325), Expect = 2.1e-26
Identity = 69/111 (62.16%), Postives = 83/111 (74.77%), Query Frame = 0


Query: 22  PLILRITSRIRSWSTRVLFFAGRLQLVRSVLQSFQVFWASVFVLPASVHHEVDRLLMSYL 81
           PLI RITSRIRSW+ RVL FAGRLQLVR VL+S QV+WASVFVLPA VHHEVD++L+SYL
Sbjct: 426 PLIQRITSRIRSWTARVLSFAGRLQLVRFVLRSLQVYWASVFVLPAYVHHEVDKILISYL 485

Query: 82  WRGKEIGRGGAKVAWTECFVVFMVMFVLLSFSGSRALASGCEDLLLLIDAS 133
           WRGKE GRGG KVAW +  + F    + +    S  +AS  + LLL++  S
Sbjct: 486 WRGKEEGRGGIKVAWVDVCLPFEESGLGIRDGPSWNIASTLKILLLMLTNS 536

[Close]

BLAST of PI0025052 vs. NCBI nr
Match: XP_031737043.1 (uncharacterized protein LOC116402131 [Cucumis sativus])

HSP 1 Score: 129.8 bits (325), Expect = 2.1e-26
Identity = 69/110 (62.73%), Postives = 81/110 (73.64%), Query Frame = 0


Query: 20  CVPLILRITSRIRSWSTRVLFFAGRLQLVRSVLQSFQVFWASVFVLPASVHHEVDRLLMS 79
           C PLI RITSRIRSWS RVL FAGRLQLVRSVL+S QV+WASVF+LP  VH +VD++L S
Sbjct: 562 CDPLIQRITSRIRSWSARVLSFAGRLQLVRSVLRSLQVYWASVFMLPMKVHRDVDKILRS 621

Query: 80  YLWRGKEIGRGGAKVAWTECFVVFMVMFVLLSFSGSRALASGCEDLLLLI 130
           YLWRGKE GRGGAKVAW E  + F    + +    S  +AS  + L LL+
Sbjct: 622 YLWRGKEEGRGGAKVAWDEVCLPFDEGGLAIRDGSSWNIASTLKILWLLL 671

[Close]

BLAST of PI0025052 vs. NCBI nr
Match: TYK28099.1 (uncharacterized protein E5676_scaffold1467G00020 [Cucumis melo var. makuwa])

HSP 1 Score: 129.4 bits (324), Expect = 2.8e-26
Identity = 62/84 (73.81%), Postives = 71/84 (84.52%), Query Frame = 0


Query: 20   CVPLILRITSRIRSWSTRVLFFAGRLQLVRSVLQSFQVFWASVFVLPASVHHEVDRLLMS 79
            C PLI RITS+IRSW+ RVL FAGRLQLVRSVL+S QV+WASVFVLPA VH+EVD++L S
Sbjct: 1040 CAPLIQRITSQIRSWNARVLSFAGRLQLVRSVLRSLQVYWASVFVLPAYVHNEVDKILRS 1099

Query: 80   YLWRGKEIGRGGAKVAWTECFVVF 104
            YLWRGKE GRGG KVAW +  + F
Sbjct: 1100 YLWRGKEEGRGGIKVAWVDVCLPF 1123

[Close]

BLAST of PI0025052 vs. NCBI nr
Match: KAA0026071.1 (uncharacterized protein E6C27_scaffold581G00620 [Cucumis melo var. makuwa])

HSP 1 Score: 129.4 bits (324), Expect = 2.8e-26
Identity = 62/84 (73.81%), Postives = 71/84 (84.52%), Query Frame = 0


Query: 20   CVPLILRITSRIRSWSTRVLFFAGRLQLVRSVLQSFQVFWASVFVLPASVHHEVDRLLMS 79
            C PLI RITS+IRSW+ RVL FAGRLQLVRSVL+S QV+WASVFVLPA VH+EVD++L S
Sbjct: 1040 CAPLIQRITSQIRSWNARVLSFAGRLQLVRSVLRSLQVYWASVFVLPAYVHNEVDKILRS 1099

Query: 80   YLWRGKEIGRGGAKVAWTECFVVF 104
            YLWRGKE GRGG KVAW +  + F
Sbjct: 1100 YLWRGKEEGRGGIKVAWVDVCLPF 1123

[Close]

BLAST of PI0025052 vs. TAIR 10
Match: AT3G24255.1 (RNA-directed DNA polymerase (reverse transcriptase)-related family protein )

HSP 1 Score: 76.6 bits (187), Expect = 2.0e-14
Identity = 36/77 (46.75%), Postives = 49/77 (63.64%), Query Frame = 0


Query: 22  PLILRITSRIRSWSTRVLFFAGRLQLVRSVLQSFQVFWASVFVLPASVHHEVDRLLMSYL 81
           PL+ +I  RI  W+ R L FAGRLQL+ SV+ S   FW S F LP++   E+D +  S+L
Sbjct: 44  PLVEKIRVRIGKWTARHLSFAGRLQLISSVIHSLTNFWMSAFRLPSACIKEIDSICSSFL 103

Query: 82  WRGKEIGRGGAKVAWTE 99
           W G E+    AKVAW++
Sbjct: 104 WSGPELNTKKAKVAWSD 120

The following BLAST results are available for this feature:

BLAST of PI0025052 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P0C2F6	9.6e-06	32.89	Putative ribonuclease H protein At1g65750 OS=Arabidopsis thaliana OX=3702 GN=At1...	[more]

BLAST of PI0025052 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7TZS0	4.6e-27	75.00	Reverse transcriptase domain-containing protein OS=Cucumis melo var. makuwa OX=1...	[more]
A0A5A7UV01	1.0e-26	62.16	F17F8.5 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold896G00020 PE=4 S...	[more]
A0A5A7SPE5	1.3e-26	73.81	Reverse transcriptase domain-containing protein OS=Cucumis melo var. makuwa OX=1...	[more]
A0A5D3DXE4	1.3e-26	73.81	Reverse transcriptase domain-containing protein OS=Cucumis melo var. makuwa OX=1...	[more]
A0A5A7V3Z0	3.9e-26	75.61	Reverse transcriptase domain-containing protein OS=Cucumis melo var. makuwa OX=1...	[more]

BLAST of PI0025052 vs. NCBI nr


Match Name	E-value	Identity	Description
KAA0046851.1	9.5e-27	75.00	uncharacterized protein E6C27_scaffold19358G00020 [Cucumis melo var. makuwa]	[more]
KAA0059752.1	2.1e-26	62.16	F17F8.5 [Cucumis melo var. makuwa] >TYJ99767.1 F17F8.5 [Cucumis melo var. makuwa...	[more]
XP_031737043.1	2.1e-26	62.73	uncharacterized protein LOC116402131 [Cucumis sativus]	[more]
TYK28099.1	2.8e-26	73.81	uncharacterized protein E5676_scaffold1467G00020 [Cucumis melo var. makuwa]	[more]
KAA0026071.1	2.8e-26	73.81	uncharacterized protein E6C27_scaffold581G00620 [Cucumis melo var. makuwa]	[more]

BLAST of PI0025052 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G24255.1	2.0e-14	46.75	RNA-directed DNA polymerase (reverse transcriptase)-related family protein	[more]

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
PI0025052.1	PI0025052.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006278	RNA-dependent DNA biosynthetic process
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0003964	RNA-directed DNA polymerase activity