PI0023939 (gene) Melon (PI 482460) v1

Overview


Name	PI0023939
Type	gene
Organism	Cucumis metuliferus (Melon (PI 482460) v1)
Description	Ribonuclease T(2)
Location	chr05: 11639839 .. 11640621 (+)
RNA-Seq Expression	PI0023939
Synteny	PI0023939

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTCAACATCTTTTCATTTTCTGTCTTTTAAGTTTGTTGTTGGTTGTGAATGACGATCCATTCAACTTCTTCCAACTAGTTGAACAATGGGGTCCAAACACATGTAATGACACTCTTGGCACTCGGTGTCATGCTCAACCTCAATCCATGTTCACTATTCATGGGCTATGGCCGAGTAACTTTTTAAATGTCCGTCTTTCTTGTGTAGTTCATATGCAATTTAAACCTAAAAAGGTTCCCATCTTCTTCGATTTTTTTTTTTGTGTGTTTTTTCTATCATTAGCATTTTAATCATTGTTTCAACATCATTTTATTTTATTTTAGGTTACTGCATTTGAACAAGATCTAAATATATATTGGCTAAATGTAAAAAGGGAGAAAAACTTTGACTTTTGGGGATATGAATGGAGAAAGCACGGTGCATGTACAAACCCTCCTTATACCGTACTTCAGTATTTTCAAATTGCTTTTTAATCTCCGAAAGAATAAAAATTATGATCTGTTAGCAATCTTAAATGATGTTGGGCTCAACCCCCAAATGCCCAAAGATCTCAAGACATCCTAAACCCTATTAAAAATGCAATAGGAAAGAATTCAGGCATCCGTTGCAACAATAATGGTATAACTGGGAAGTTGCAATTATATGAAATTGTACTGTGTTTTGACAAGAATCTTGTTACCCTCATTGACTGCCCTCGGTTTGTTAGCAACAGTTGTCCATCTACATTTGTGTGGCTCCTCCCACAACAGAGTTCAGTTGGTGTTCCAAATTTTCCATGA

mRNA sequence

ATGGCTCAACATCTTTTCATTTTCTGTCTTTTAAGTTTGTTGTTGGTTGTGAATGACGATCCATTCAACTTCTTCCAACTAGTTGAACAATGGGGTCCAAACACATGTAATGACACTCTTGGCACTCGGTGTCATGCTCAACCTCAATCCATGTTCACTATTCATGGGCTATGGCCGAGTAACTTTTTAAATGTCCGTCTTTCTTGTGTAGTTCATATGCAATTTAAACCTAAAAAGGTTACTGCATTTGAACAAGATCTAAATATATATTGGCTAAATGTAAAAAGGGAGAAAAACTTTGACTTTTGGGGATATGAATGGAGAAAGCACGGTGCATGTACAAACCCTCCTTATACCGTACTTCAATCTCAAGACATCCTAAACCCTATTAAAAATGCAATAGGAAAGAATTCAGGCATCCGTTGCAACAATAATGGTATAACTGGGAAGTTGCAATTATATGAAATTGTACTGTGTTTTGACAAGAATCTTGTTACCCTCATTGACTGCCCTCGGTTTGTTAGCAACAGTTGTCCATCTACATTTGTGTGGCTCCTCCCACAACAGAGTTCAGTTGGTGTTCCAAATTTTCCATGA

Coding sequence (CDS)

ATGGCTCAACATCTTTTCATTTTCTGTCTTTTAAGTTTGTTGTTGGTTGTGAATGACGATCCATTCAACTTCTTCCAACTAGTTGAACAATGGGGTCCAAACACATGTAATGACACTCTTGGCACTCGGTGTCATGCTCAACCTCAATCCATGTTCACTATTCATGGGCTATGGCCGAGTAACTTTTTAAATGTCCGTCTTTCTTGTGTAGTTCATATGCAATTTAAACCTAAAAAGGTTACTGCATTTGAACAAGATCTAAATATATATTGGCTAAATGTAAAAAGGGAGAAAAACTTTGACTTTTGGGGATATGAATGGAGAAAGCACGGTGCATGTACAAACCCTCCTTATACCGTACTTCAATCTCAAGACATCCTAAACCCTATTAAAAATGCAATAGGAAAGAATTCAGGCATCCGTTGCAACAATAATGGTATAACTGGGAAGTTGCAATTATATGAAATTGTACTGTGTTTTGACAAGAATCTTGTTACCCTCATTGACTGCCCTCGGTTTGTTAGCAACAGTTGTCCATCTACATTTGTGTGGCTCCTCCCACAACAGAGTTCAGTTGGTGTTCCAAATTTTCCATGA

Protein sequence

MAQHLFIFCLLSLLLVVNDDPFNFFQLVEQWGPNTCNDTLGTRCHAQPQSMFTIHGLWPSNFLNVRLSCVVHMQFKPKKVTAFEQDLNIYWLNVKREKNFDFWGYEWRKHGACTNPPYTVLQSQDILNPIKNAIGKNSGIRCNNNGITGKLQLYEIVLCFDKNLVTLIDCPRFVSNSCPSTFVWLLPQQSSVGVPNFP

Homology

[Close]

BLAST of PI0023939 vs. ExPASy Swiss-Prot
Match: P23540 (Ribonuclease MC OS=Momordica charantia OX=3673 PE=1 SV=1)

HSP 1 Score: 95.5 bits (236), Expect = 7.1e-19
Identity = 55/194 (28.35%), Postives = 80/194 (41.24%), Query Frame = 0


Query: 22  FNFFQLVEQWGPNTCNDTLGTRCHAQPQSMFTIHGLWPSNFLNVRLSCVVHMQFKPKKVT 81
           F+ F  V+QW P  C+      C       FTIHGLWP        +C     F   K++
Sbjct: 1   FDSFWFVQQWPPAVCSFQKSGSCPGSGLRTFTIHGLWPQGSGTSLTNCPQGSPFDITKIS 60

Query: 82  AFEQDLNIYWLNVKREKNFDFWGYEWRKHGACTNPPYT----------VLQSQDILNP-- 141
             +  LN  W NV R  N  FW +EW KHG C+   +           +  + DI+    
Sbjct: 61  HLQSQLNTLWPNVLRANNQQFWSHEWTKHGTCSESTFNQAAYFKLAVDMRNNYDIIGALR 120

Query: 142 -------------------IKNAIGKNSGIRCNNNGITGKLQLYEIVLCFDKNLVTLIDC 185
                              +K   GK  G+RC  +  T    L ++V CF ++  TLIDC
Sbjct: 121 PHAAGPNGRTKSRQAIKGFLKAKFGKFPGLRCRTDPQTKVSYLVQVVACFAQDGSTLIDC 180

[Close]

BLAST of PI0023939 vs. ExPASy Swiss-Prot
Match: O80324 (Ribonuclease S-6 OS=Pyrus pyrifolia OX=3767 PE=1 SV=1)

HSP 1 Score: 90.5 bits (223), Expect = 2.3e-17
Identity = 57/203 (28.08%), Postives = 84/203 (41.38%), Query Frame = 0


Query: 7   IFCLLSLLLVVNDDPFNFFQLVEQWGPNTCNDTLGTRCHAQPQSMFTIHGLWPSNFLNVR 66
           +F L+ L+   +   +N+FQ  +Q+ P  CN    T C   P  +FT+HGLWPSN +   
Sbjct: 13  VFSLIVLISCSSTMGYNYFQFTQQYQPAVCNSN-PTPCKDPPDKLFTVHGLWPSNDVGDD 72

Query: 67  LSCVVHMQFKPKKVTAFEQDLNIYWLNV-KREKNFDFWGYEWRKHGAC------------ 126
                +   K +++      L I W NV  R  +  FW  +W KHG+C            
Sbjct: 73  PIYCKNKTIKSQQIGNLTAQLIIIWPNVLDRTDHVGFWNRQWNKHGSCGKAPTIKDEMHY 132

Query: 127 -----------------------TNPPYTVLQSQDILNPIK-NAIGKNSGIRCNNNGITG 173
                                    P   + +  DI+N I+     K   ++C  N  T 
Sbjct: 133 FKTVIKMYITQKQNVSEILSRAKIEPEGKIRRRDDIINAIRLGTKDKKPKLKCQKNNQT- 192

[Close]

BLAST of PI0023939 vs. ExPASy Swiss-Prot
Match: O80323 (Ribonuclease S-3 OS=Pyrus pyrifolia OX=3767 PE=1 SV=1)

HSP 1 Score: 88.2 bits (217), Expect = 1.1e-16
Identity = 66/204 (32.35%), Postives = 90/204 (44.12%), Query Frame = 0


Query: 5   LFIFCLLSLLLVVNDDPFNFFQLVEQWGPNTCNDTLGTRCHAQPQSMFTIHGLWPSNFLN 64
           + +F L+ L+L  +   +++FQ  +Q+    CN    T C   P  +FT+HGLWPSN + 
Sbjct: 6   MMVFLLIVLILCSSTVGYDYFQFTQQYQLAVCNSN-RTLCKDPPDKLFTVHGLWPSNMVG 65

Query: 65  VRLS-CVVHMQFKPKKVTAFEQDLNIYWLNV-KREKNFDFWGYEWRKHGACTNPPY---- 124
              S C +    K +K+   E  L I W NV  R KN  FW  EW KHG+C  P      
Sbjct: 66  PDPSKCPIKNIRKREKL--LEHQLEIIWPNVFDRTKNNLFWDKEWMKHGSCGYPTIDNEN 125

Query: 125 ----TVLQSQ--------------------------DILNPIKN-AIGKNSGIRCNNNGI 172
               TV++                            DI N I+N A  K   ++C   G 
Sbjct: 126 HYFETVIKMYISKKQNVSRILSKAKIEPDGKKRALLDIENAIRNGADNKKPKLKCQKKGT 185

[Close]

BLAST of PI0023939 vs. ExPASy Swiss-Prot
Match: Q40965 (Ribonuclease S-2 OS=Pyrus pyrifolia OX=3767 PE=1 SV=1)

HSP 1 Score: 85.9 bits (211), Expect = 5.6e-16
Identity = 60/207 (28.99%), Postives = 91/207 (43.96%), Query Frame = 0


Query: 7   IFCLLSLLLVVNDDPFNFFQLVEQWGPNTCNDTLGTRCHAQPQSMFTIHGLWPSNFLNVR 66
           +F L  L+L  +   +++FQ  +Q+    CN    T C   P  +FT+HGLWPS  +   
Sbjct: 8   VFSLNVLILSSSAARYDYFQFTQQYQQAFCNSN-PTPCKDPPDKLFTVHGLWPSTKVGRD 67

Query: 67  LSCVVHMQFKPKKVTAFEQDLNIYWLNVK-REKNFDFWGYEWRKHGACTNPPY------- 126
                  ++  +K+   E  L I W NV  R+ N  FW  +W KHG+C +P         
Sbjct: 68  PEYCKTKRY--RKIQRLEPQLEIIWPNVSDRKANRGFWRKQWYKHGSCASPALPNQKHYF 127

Query: 127 -TVL-----QSQDILNPIKNAI----GKN-------SGIRCNNNG---------ITGKLQ 180
            TV+     + Q++   +  A     GKN       + IR   N          + G  +
Sbjct: 128 ETVIRMFLAEKQNVSRILSMATIEPEGKNRTLLEIQNAIRAGTNNMIPKLKCQKVNGMTE 187

[Close]

BLAST of PI0023939 vs. ExPASy Swiss-Prot
Match: O80325 (Ribonuclease S-7 OS=Pyrus pyrifolia OX=3767 PE=1 SV=1)

HSP 1 Score: 85.5 bits (210), Expect = 7.4e-16
Identity = 56/201 (27.86%), Postives = 85/201 (42.29%), Query Frame = 0


Query: 7   IFCLLSLLLVVNDDPFNFFQLVEQWGPNTCNDTLGTRCHAQPQSMFTIHGLWPSNFLNVR 66
           +F L+ L+L  +   +++FQ  +Q+ P  CN +  T C   P  +FT+HGLWPSN     
Sbjct: 13  VFSLIVLILSSSTVGYDYFQFTQQYQPAVCN-SKPTPCKDPPDKLFTVHGLWPSNLNGPH 72

Query: 67  LSCVVHMQFKPKKVTAFEQDLNIYWLNV-KREKNFDFWGYEWRKHGACTNPPYT------ 126
                +    P ++   +  L I W NV  R  +  FW  +W KHG+C  P         
Sbjct: 73  PENCTNATVNPHRIKNIQAQLKIIWPNVLDRTNHVGFWNKQWIKHGSCGYPAIMNDTHYF 132

Query: 127 -------VLQSQDI-----------------LNPIKNAI-----GKNSGIRCNNNGITGK 172
                  + Q Q++                 L  I+NAI      K    +C  N  +G 
Sbjct: 133 QTVINMYITQKQNVSEILSKAKIEPLGIQRPLVHIENAIRNSTNNKKPKFKCQKN--SGV 192

[Close]

BLAST of PI0023939 vs. ExPASy TrEMBL
Match: A0A5D3DP91 (Ribonuclease T(2) OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G002140 PE=3 SV=1)

HSP 1 Score: 256.9 bits (655), Expect = 6.8e-65
Identity = 130/232 (56.03%), Postives = 147/232 (63.36%), Query Frame = 0


Query: 1   MAQHLFIFCLLSLLLVVNDDPFNFFQLVEQWGPNTCNDTLGTRCHAQPQSMFTIHGLWPS 60
           MAQH FI CLLSLLL VN  PF+FFQLVEQWGPNTCND  GT+CHAQPQ  FTIHGLWPS
Sbjct: 1   MAQHTFILCLLSLLLFVNGYPFDFFQLVEQWGPNTCND--GTQCHAQPQPEFTIHGLWPS 60

Query: 61  NFLNVRLSCVVHMQFKPKKVTAFEQDLNIYWLNVKREKNFDFWGYEWRKHGACTNPPYTV 120
           NF N RL C     F   +++  E DL+ YW +V    N  FW  EW+KHGAC +PP+ +
Sbjct: 61  NFSNARLFCATRKLFDNGQISLLEGDLDRYWPDVINGNNRGFWSNEWKKHGACIDPPFNI 120

Query: 121 LQ----------------------------------SQDILNPIKNAIGKNSGIRCNNNG 180
            Q                                   +DILNPI NA  K +GIRCN NG
Sbjct: 121 TQYFQLSLDHHRTYDLLKILNGVWHRNRQMPNNGLAPKDILNPIFNATNKTAGIRCNKNG 180

Query: 181 ITGKLQLYEIVLCFDKNLVTLIDCPRFVSNSC-PSTFVWLLPQQSSVGVPNF 198
           IT KLQL+EIVLCFD N  TLIDCP+FVSNSC    FVWLLPQQSS GVP++
Sbjct: 181 ITKKLQLHEIVLCFDNNGTTLIDCPKFVSNSCNQPKFVWLLPQQSSDGVPDY 230

[Close]

BLAST of PI0023939 vs. ExPASy TrEMBL
Match: A0A0A0LAB0 (Ribonuclease T(2) OS=Cucumis sativus OX=3659 GN=Csa_3G736680 PE=3 SV=1)

HSP 1 Score: 244.2 bits (622), Expect = 4.6e-61
Identity = 127/235 (54.04%), Postives = 151/235 (64.26%), Query Frame = 0


Query: 1   MAQHLFIFCLLSLLLVVNDDPFNFFQLVEQWGPNTCNDTLGTRCHAQPQSMFTIHGLWPS 60
           MAQH+FI CLLS LL VN  PF+FFQLV+QWGPN C+   G++C+AQPQ MFTIHGLWPS
Sbjct: 1   MAQHIFILCLLSSLLFVNGIPFDFFQLVQQWGPNICSPA-GSKCYAQPQPMFTIHGLWPS 60

Query: 61  NFLNVRLSCVVHMQFKPK-KVTAFEQDLNIYWLNVKREKNFDFWGYEWRKHGACTNPPYT 120
           NF  V + C    +  P+ KVT+ EQ LN YW +V   KN +FW +EWRKH  C +PP+T
Sbjct: 61  NFSTVPILCPRTKKLFPQGKVTSLEQHLNKYWPDVISGKNSNFWEHEWRKHADCIDPPFT 120

Query: 121 V----------------------------------LQSQDILNPIKNAIGKNSGIRCNNN 180
           +                                  L SQ++L+PIKNA G   GIRCN N
Sbjct: 121 IFWYFDISLRHRMNKTYDLLTILNDAGLSHQTHKDLISQNVLDPIKNATGMEPGIRCNIN 180

Query: 181 GITGKLQLYEIVLCFDKNLVTLIDCPRFVSNSCPS--TFVWLLPQQSSVGVPNFP 199
           GIT KLQL EIVLCF  +  TLIDCP FVSN+C S   FVWLLPQQSSVGVP++P
Sbjct: 181 GITKKLQLKEIVLCFGNDGTTLIDCPSFVSNTCTSQPKFVWLLPQQSSVGVPDYP 234

[Close]

BLAST of PI0023939 vs. ExPASy TrEMBL
Match: A0A0A0LAN6 (Ribonuclease T(2) OS=Cucumis sativus OX=3659 GN=Csa_3G745530 PE=3 SV=1)

HSP 1 Score: 177.6 bits (449), Expect = 5.3e-41
Identity = 97/220 (44.09%), Postives = 118/220 (53.64%), Query Frame = 0


Query: 1   MAQHLFIFCLLSLLLVVNDD-PFNFFQLVEQWGPNTCNDTLGTR---CHAQPQSMFTIHG 60
           M Q + I   LSL++ VN   PF FFQ VEQWGPN CN   G R   CH  PQ+ FTIHG
Sbjct: 1   MTQSILIIYFLSLVVFVNGGVPFEFFQFVEQWGPNVCN--TGARVSPCHTPPQAKFTIHG 60

Query: 61  LWPSNFLNVRLSCVVHMQFKPKKVTAFEQDLNIYWLNVKREKNFDFWGYEWRKHGACTNP 120
           LWPSN  N  L C     +   KV + E  LNI W ++K   N  FWG+EW  HG C++P
Sbjct: 61  LWPSNHSNAHLLCQSKTIY--SKVISLEGQLNISWPDLKYGNNDKFWGHEWDTHGKCSDP 120

Query: 121 PYTVLQ--------------------------------SQDILNPIKNAIGKNSGIRCNN 180
           P+++ Q                                 Q+I+ PI+    K  GIRCN 
Sbjct: 121 PFSLFQYFQISLNLLRKFDLLTILKAAGLNPQTSQNLAIQNIMAPIQRTTKKYPGIRCNK 180

Query: 181 NGITGKLQLYEIVLCFDKNLVTLIDCPRFVSNSCPSTFVW 185
           N  TGK QL EIVLC +K+  TLIDCP FVSN+C  +FVW
Sbjct: 181 NVKTGKSQLNEIVLCLEKDGATLIDCPTFVSNTCAKSFVW 216

[Close]

BLAST of PI0023939 vs. ExPASy TrEMBL
Match: A0A6J1EXD5 (Ribonuclease T(2) OS=Cucurbita moschata OX=3662 GN=LOC111439251 PE=3 SV=1)

HSP 1 Score: 168.3 bits (425), Expect = 3.2e-38
Identity = 90/221 (40.72%), Postives = 118/221 (53.39%), Query Frame = 0


Query: 1   MAQHLFIFCLLSLLLVVN--DDPFNFFQLVEQWGPNTCNDTLGTRCHAQPQSMFTIHGLW 60
           MAQ  FI  +   +L+ +     F++FQ+V+QW P TC+   G RC+A P +MFTIHGLW
Sbjct: 1   MAQAPFIVSIFKFVLLTSCVKAQFDYFQMVQQWPPATCS---GVRCYANPPAMFTIHGLW 60

Query: 61  PSNFLNVRLSCVVHMQFKPKKVTAFEQDLNIYWLNVKREKNFDFWGYEWRKHGACTNPPY 120
           PSN+ NV L C     F P ++      LN YW +V    N  FW +EW  HG C++PP+
Sbjct: 61  PSNYSNVPLVC-AGQPFNPAQIATLVPQLNTYWPDVISGNNQRFWKHEWDSHGTCSDPPF 120

Query: 121 TVLQ------------SQDIL----------------------NPIKNAIGKNSGIRCNN 180
             LQ            + D+L                        I+ A GK  G+RCN 
Sbjct: 121 NQLQYFQTTLNIRTNHNYDLLAILNTAGLGPTATNTRQYGAIEGAIQAATGKKPGLRCNK 180

Query: 181 NGITGKLQLYEIVLCFDKNLVTLIDCPRFVSNSCPSTFVWL 186
           N  + K QL+EI+LCFDKN VTLIDC +F   +CPS FVWL
Sbjct: 181 NAQSKKKQLFEIILCFDKNGVTLIDCTQFAGITCPSQFVWL 217

[Close]

BLAST of PI0023939 vs. ExPASy TrEMBL
Match: A0A6J1F837 (Ribonuclease T(2) OS=Cucurbita moschata OX=3662 GN=LOC111441728 PE=3 SV=1)

HSP 1 Score: 166.4 bits (420), Expect = 1.2e-37
Identity = 88/215 (40.93%), Postives = 116/215 (53.95%), Query Frame = 0


Query: 5   LFIFCLLSLLLVVNDDPFNFFQLVEQWGPNTCNDTLGTRCHAQPQSMFTIHGLWPSNFLN 64
           +F F  + L+L VN   F++FQ+V+QW P  C+ ++  RCH QP +MFTIHGLWPSN+ N
Sbjct: 10  IFNFVFVVLILCVNAQ-FDYFQMVQQWPPAKCS-SVKVRCHVQPPTMFTIHGLWPSNYSN 69

Query: 65  VRLSCVVHMQFKPKKVTAFEQDLNIYWLNVKREKNFDFWGYEWRKHGACTNPPYTVLQ-- 124
            RL C     F P ++ A    LN YW +V + KN  FW +EW +HG C+NPP+  LQ  
Sbjct: 70  ARLVC-APQPFNPTQIAALRPQLNTYWPDVIQGKNQQFWQHEWDRHGICSNPPFNQLQYF 129

Query: 125 --------------------------------SQDILNPIKNAIGKNSGIRCNNNGIT-G 184
                                            +DI   I+ A GK  G+RCN N  +  
Sbjct: 130 QITLNIRTNHSYDLLTILNAAGLGPSDTNIREYKDIEGAIQAATGKKPGLRCNINAQSKE 189

[Close]

BLAST of PI0023939 vs. NCBI nr
Match: TYK25090.1 (S-RNase [Cucumis melo var. makuwa])

HSP 1 Score: 256.9 bits (655), Expect = 1.4e-64
Identity = 130/232 (56.03%), Postives = 147/232 (63.36%), Query Frame = 0


Query: 1   MAQHLFIFCLLSLLLVVNDDPFNFFQLVEQWGPNTCNDTLGTRCHAQPQSMFTIHGLWPS 60
           MAQH FI CLLSLLL VN  PF+FFQLVEQWGPNTCND  GT+CHAQPQ  FTIHGLWPS
Sbjct: 1   MAQHTFILCLLSLLLFVNGYPFDFFQLVEQWGPNTCND--GTQCHAQPQPEFTIHGLWPS 60

Query: 61  NFLNVRLSCVVHMQFKPKKVTAFEQDLNIYWLNVKREKNFDFWGYEWRKHGACTNPPYTV 120
           NF N RL C     F   +++  E DL+ YW +V    N  FW  EW+KHGAC +PP+ +
Sbjct: 61  NFSNARLFCATRKLFDNGQISLLEGDLDRYWPDVINGNNRGFWSNEWKKHGACIDPPFNI 120

Query: 121 LQ----------------------------------SQDILNPIKNAIGKNSGIRCNNNG 180
            Q                                   +DILNPI NA  K +GIRCN NG
Sbjct: 121 TQYFQLSLDHHRTYDLLKILNGVWHRNRQMPNNGLAPKDILNPIFNATNKTAGIRCNKNG 180

Query: 181 ITGKLQLYEIVLCFDKNLVTLIDCPRFVSNSC-PSTFVWLLPQQSSVGVPNF 198
           IT KLQL+EIVLCFD N  TLIDCP+FVSNSC    FVWLLPQQSS GVP++
Sbjct: 181 ITKKLQLHEIVLCFDNNGTTLIDCPKFVSNSCNQPKFVWLLPQQSSDGVPDY 230

[Close]

BLAST of PI0023939 vs. NCBI nr
Match: XP_011651927.1 (ribonuclease MC [Cucumis sativus] >KGN58920.1 hypothetical protein Csa_001884 [Cucumis sativus])

HSP 1 Score: 244.2 bits (622), Expect = 9.5e-61
Identity = 127/235 (54.04%), Postives = 151/235 (64.26%), Query Frame = 0


Query: 1   MAQHLFIFCLLSLLLVVNDDPFNFFQLVEQWGPNTCNDTLGTRCHAQPQSMFTIHGLWPS 60
           MAQH+FI CLLS LL VN  PF+FFQLV+QWGPN C+   G++C+AQPQ MFTIHGLWPS
Sbjct: 1   MAQHIFILCLLSSLLFVNGIPFDFFQLVQQWGPNICSPA-GSKCYAQPQPMFTIHGLWPS 60

Query: 61  NFLNVRLSCVVHMQFKPK-KVTAFEQDLNIYWLNVKREKNFDFWGYEWRKHGACTNPPYT 120
           NF  V + C    +  P+ KVT+ EQ LN YW +V   KN +FW +EWRKH  C +PP+T
Sbjct: 61  NFSTVPILCPRTKKLFPQGKVTSLEQHLNKYWPDVISGKNSNFWEHEWRKHADCIDPPFT 120

Query: 121 V----------------------------------LQSQDILNPIKNAIGKNSGIRCNNN 180
           +                                  L SQ++L+PIKNA G   GIRCN N
Sbjct: 121 IFWYFDISLRHRMNKTYDLLTILNDAGLSHQTHKDLISQNVLDPIKNATGMEPGIRCNIN 180

Query: 181 GITGKLQLYEIVLCFDKNLVTLIDCPRFVSNSCPS--TFVWLLPQQSSVGVPNFP 199
           GIT KLQL EIVLCF  +  TLIDCP FVSN+C S   FVWLLPQQSSVGVP++P
Sbjct: 181 GITKKLQLKEIVLCFGNDGTTLIDCPSFVSNTCTSQPKFVWLLPQQSSVGVPDYP 234

[Close]

BLAST of PI0023939 vs. NCBI nr
Match: XP_038906187.1 (ribonuclease MC-like [Benincasa hispida])

HSP 1 Score: 179.1 bits (453), Expect = 3.7e-41
Identity = 97/230 (42.17%), Postives = 123/230 (53.48%), Query Frame = 0


Query: 1   MAQHLFIFCLLSLLLVVND--DPFNFFQLVEQWGPNTCNDTLGTRCHAQPQSMFTIHGLW 60
           MA+ L +  + SL L+V      +++FQ+V+QW P TC+  +G RC AQP SMFTIHGLW
Sbjct: 1   MAKALLVISIFSLALLVFSVKAQYDYFQMVQQWPPATCSG-VGVRCRAQPPSMFTIHGLW 60

Query: 61  PSNFLNVRLSCVVHMQFKPKKVTAFEQDLNIYWLNVKREKNFDFWGYEWRKHGACTNPPY 120
           PSN+    L C   + F P ++ A    LN YW +V R  N  FW +EW  HG C++PP+
Sbjct: 61  PSNYSTAHLVC-TGLPFDPTQIAAIRPQLNTYWPDVIRGNNQRFWQHEWDAHGTCSDPPF 120

Query: 121 T----------------------------------VLQSQDILNPIKNAIGKNSGIRCNN 180
                                              V Q Q I N I+ A GK  G+RCN 
Sbjct: 121 NQRQYFQTTLNIRMNHNYDLLAILNAAGLGPTATMVRQYQAIENAIQAATGKIPGLRCNT 180

Query: 181 NGITGKLQLYEIVLCFDKNLVTLIDCPRFVSNSCPSTFVWLLPQQSSVGV 195
           N  TG  QL+EI+LCFDKN VTLIDC  F   +CP  FVWL  Q  S+GV
Sbjct: 181 NAQTGSRQLFEIILCFDKNGVTLIDCTPFAGITCPLQFVWLHRQPWSLGV 228

[Close]

BLAST of PI0023939 vs. NCBI nr
Match: KAE8651058.1 (hypothetical protein Csa_002465 [Cucumis sativus])

HSP 1 Score: 177.6 bits (449), Expect = 1.1e-40
Identity = 97/220 (44.09%), Postives = 118/220 (53.64%), Query Frame = 0


Query: 1   MAQHLFIFCLLSLLLVVNDD-PFNFFQLVEQWGPNTCNDTLGTR---CHAQPQSMFTIHG 60
           M Q + I   LSL++ VN   PF FFQ VEQWGPN CN   G R   CH  PQ+ FTIHG
Sbjct: 1   MTQSILIIYFLSLVVFVNGGVPFEFFQFVEQWGPNVCN--TGARVSPCHTPPQAKFTIHG 60

Query: 61  LWPSNFLNVRLSCVVHMQFKPKKVTAFEQDLNIYWLNVKREKNFDFWGYEWRKHGACTNP 120
           LWPSN  N  L C     +   KV + E  LNI W ++K   N  FWG+EW  HG C++P
Sbjct: 61  LWPSNHSNAHLLCQSKTIY--SKVISLEGQLNISWPDLKYGNNDKFWGHEWDTHGKCSDP 120

Query: 121 PYTVLQ--------------------------------SQDILNPIKNAIGKNSGIRCNN 180
           P+++ Q                                 Q+I+ PI+    K  GIRCN 
Sbjct: 121 PFSLFQYFQISLNLLRKFDLLTILKAAGLNPQTSQNLAIQNIMAPIQRTTKKYPGIRCNK 180

Query: 181 NGITGKLQLYEIVLCFDKNLVTLIDCPRFVSNSCPSTFVW 185
           N  TGK QL EIVLC +K+  TLIDCP FVSN+C  +FVW
Sbjct: 181 NVKTGKSQLNEIVLCLEKDGATLIDCPTFVSNTCAKSFVW 216

[Close]

BLAST of PI0023939 vs. NCBI nr
Match: XP_031738690.1 (ribonuclease MC-like [Cucumis sativus])

HSP 1 Score: 177.6 bits (449), Expect = 1.1e-40
Identity = 97/220 (44.09%), Postives = 118/220 (53.64%), Query Frame = 0


Query: 1   MAQHLFIFCLLSLLLVVNDD-PFNFFQLVEQWGPNTCNDTLGTR---CHAQPQSMFTIHG 60
           M Q + I   LSL++ VN   PF FFQ VEQWGPN CN   G R   CH  PQ+ FTIHG
Sbjct: 1   MTQSILIIYFLSLVVFVNGGVPFEFFQFVEQWGPNVCN--TGARVSPCHTPPQAKFTIHG 60

Query: 61  LWPSNFLNVRLSCVVHMQFKPKKVTAFEQDLNIYWLNVKREKNFDFWGYEWRKHGACTNP 120
           LWPSN  N  L C     +   KV + E  LNI W ++K   N  FWG+EW  HG C++P
Sbjct: 61  LWPSNHSNAHLLCQSKTIY--SKVISLEGQLNISWPDLKYGNNDKFWGHEWDTHGKCSDP 120

Query: 121 PYTVLQ--------------------------------SQDILNPIKNAIGKNSGIRCNN 180
           P+++ Q                                 Q+I+ PI+    K  GIRCN 
Sbjct: 121 PFSLFQYFQISLNLLRKFDLLTILKAAGLNPQTSQNLAIQNIMAPIQRTTKKYPGIRCNK 180

Query: 181 NGITGKLQLYEIVLCFDKNLVTLIDCPRFVSNSCPSTFVW 185
           N  TGK QL EIVLC +K+  TLIDCP FVSN+C  +FVW
Sbjct: 181 NVKTGKSQLNEIVLCLEKDGATLIDCPTFVSNTCAKSFVW 216

[Close]

BLAST of PI0023939 vs. TAIR 10
Match: AT1G14220.1 (Ribonuclease T2 family protein )

HSP 1 Score: 85.5 bits (210), Expect = 5.2e-17
Identity = 58/198 (29.29%), Postives = 81/198 (40.91%), Query Frame = 0


Query: 20  DPFNFFQLVEQWGPNTCNDTLGTRCH---AQPQSMFTIHGLWPS-NFLNVRLSCVVHMQF 79
           + F+FF  V QW P    DT    C+    +P + F IHGLWP+ N  +   +C    QF
Sbjct: 26  EDFDFFYFVLQW-PGAYCDTSRACCYPTSGKPAADFGIHGLWPNYNGGSWPSNCDPDSQF 85

Query: 80  KPKKVTAFEQDLNIYW--LNVKREKNFDFWGYEWRKHGACT------------------- 139
              +++     L   W  L+    + F+FW +EW KHG C+                   
Sbjct: 86  DRSQISDLVSSLKKNWPTLSCPSNEGFNFWEHEWEKHGTCSESVMDQHEYFENALKLKQK 145

Query: 140 ------------NPPYTVLQSQDILNPIKNAIGKNSGIRCNNNGITGKLQLYEIVLCFDK 181
                       NP         I N IK+ IG   GI CN +      QL++I +C D 
Sbjct: 146 ANLLQILKNSGINPDDGFYNLDKITNAIKDGIGFTPGIECNKDP-ERNAQLHQIYICVDT 205

[Close]

BLAST of PI0023939 vs. TAIR 10
Match: AT1G14210.1 (Ribonuclease T2 family protein )

HSP 1 Score: 66.6 bits (161), Expect = 2.5e-11
Identity = 54/210 (25.71%), Postives = 72/210 (34.29%), Query Frame = 0


Query: 22  FNFFQLVEQWGPNTCNDTLG--TRCHAQPQSMFTIHGLWPS-NFLNVRLSCVVHMQFKPK 81
           FNFF  V  W    C+   G          S F IHGLWP  N       C     F   
Sbjct: 26  FNFFYWVNYWPGAICDSQKGCCPPTKGNTASDFIIHGLWPQFNNGTWPAFCDQTNLFDIS 85

Query: 82  KVTAFEQDLNIYWLN----VKREKNFDFWGYEWRKHGACT-------------------- 141
           K++     +   W             + W +EW KHG C                     
Sbjct: 86  KISDLVCQMEKKWTEWGVWACPSNETNLWEHEWNKHGTCVQSIFDQHSYFRTNLKFKHKV 145

Query: 142 -----------NPPYTVLQSQDILNPIKNAIGKNSGIRCNNNGITGKLQLYEIVLCFDKN 193
                       P        +I N IK AIG   GI CN + + G  QL++I +C D  
Sbjct: 146 HLLNILIQKGIKPNDGFYSLDEIKNAIKCAIGFAPGIECNED-VKGNKQLFQIYICLDNY 205

[Close]

BLAST of PI0023939 vs. TAIR 10
Match: AT2G39780.1 (ribonuclease 2 )

HSP 1 Score: 63.5 bits (153), Expect = 2.1e-10
Identity = 34/110 (30.91%), Postives = 47/110 (42.73%), Query Frame = 0


Query: 22  FNFFQLVEQWGPNTCNDT-----LGTRCH-AQPQSMFTIHGLWPSNFLNVRLSCVVHMQF 81
           F++F L  QW    C  T         C  +   + FTIHGLWP        SC     F
Sbjct: 32  FDYFALSLQWPGTYCRGTRHCCSKNACCRGSDAPTQFTIHGLWPDYNDGSWPSCCYRSDF 91

Query: 82  KPKKVTAFEQDLNIYWLNVK-------REKNFDFWGYEWRKHGACTNPPY 119
           K K+++     L  YW ++              FWG+EW KHG C++P +
Sbjct: 92  KEKEISTLMDGLEKYWPSLSCGSPSSCNGGKGSFWGHEWEKHGTCSSPVF 141

[Close]

BLAST of PI0023939 vs. TAIR 10
Match: AT2G39780.2 (ribonuclease 2 )

HSP 1 Score: 50.4 bits (119), Expect = 1.9e-06
Identity = 46/183 (25.14%), Postives = 66/183 (36.07%), Query Frame = 0


Query: 22  FNFFQLVEQWGPNTCNDT-----LGTRCH-AQPQSMFTIHGLWPSNFLNVRLSCVVHMQF 81
           F++F L  QW    C  T         C  +   + FTIHGLWP        SC     F
Sbjct: 32  FDYFALSLQWPGTYCRGTRHCCSKNACCRGSDAPTQFTIHGLWPDYNDGSWPSCCYRSDF 91

Query: 82  KPKKVTAFEQDLNIYWLNVK-------REKNFDFWGYEWRKHGACTNPPYTVLQSQ---- 141
           K K+++     L  YW ++              FWG+E           Y    S+    
Sbjct: 92  KEKEISTLMDGLEKYWPSLSCGSPSSCNGGKGSFWGHE----DVLYQAGYVASNSEKYPL 151

Query: 142 -DILNPIKNAIGKNSGIRCNNNGITGKLQLYEIVLCFDK-----------NLVTLIDCPR 176
             I+  I+NA      + C  + I       EI +CF K           +L +   CP+
Sbjct: 152 GGIVTAIQNAFHITPEVVCKRDAID------EIRICFYKDFKPRDCVGSQDLTSRKSCPK 204

The following BLAST results are available for this feature:

BLAST of PI0023939 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P23540	7.1e-19	28.35	Ribonuclease MC OS=Momordica charantia OX=3673 PE=1 SV=1	[more]
O80324	2.3e-17	28.08	Ribonuclease S-6 OS=Pyrus pyrifolia OX=3767 PE=1 SV=1	[more]
O80323	1.1e-16	32.35	Ribonuclease S-3 OS=Pyrus pyrifolia OX=3767 PE=1 SV=1	[more]
Q40965	5.6e-16	28.99	Ribonuclease S-2 OS=Pyrus pyrifolia OX=3767 PE=1 SV=1	[more]
O80325	7.4e-16	27.86	Ribonuclease S-7 OS=Pyrus pyrifolia OX=3767 PE=1 SV=1	[more]

BLAST of PI0023939 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3DP91	6.8e-65	56.03	Ribonuclease T(2) OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G00...	[more]
A0A0A0LAB0	4.6e-61	54.04	Ribonuclease T(2) OS=Cucumis sativus OX=3659 GN=Csa_3G736680 PE=3 SV=1	[more]
A0A0A0LAN6	5.3e-41	44.09	Ribonuclease T(2) OS=Cucumis sativus OX=3659 GN=Csa_3G745530 PE=3 SV=1	[more]
A0A6J1EXD5	3.2e-38	40.72	Ribonuclease T(2) OS=Cucurbita moschata OX=3662 GN=LOC111439251 PE=3 SV=1	[more]
A0A6J1F837	1.2e-37	40.93	Ribonuclease T(2) OS=Cucurbita moschata OX=3662 GN=LOC111441728 PE=3 SV=1	[more]

BLAST of PI0023939 vs. NCBI nr


Match Name	E-value	Identity	Description
TYK25090.1	1.4e-64	56.03	S-RNase [Cucumis melo var. makuwa]	[more]
XP_011651927.1	9.5e-61	54.04	ribonuclease MC [Cucumis sativus] >KGN58920.1 hypothetical protein Csa_001884 [C...	[more]
XP_038906187.1	3.7e-41	42.17	ribonuclease MC-like [Benincasa hispida]	[more]
KAE8651058.1	1.1e-40	44.09	hypothetical protein Csa_002465 [Cucumis sativus]	[more]
XP_031738690.1	1.1e-40	44.09	ribonuclease MC-like [Cucumis sativus]	[more]

BLAST of PI0023939 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G14220.1	5.2e-17	29.29	Ribonuclease T2 family protein	[more]
AT1G14210.1	2.5e-11	25.71	Ribonuclease T2 family protein	[more]
AT2G39780.1	2.1e-10	30.91	ribonuclease 2	[more]
AT2G39780.2	1.9e-06	25.14	ribonuclease 2	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036430	Ribonuclease T2-like superfamily	GENE3D	3.90.730.10		coord: 16..119 e-value: 5.3E-24 score: 87.2
IPR036430	Ribonuclease T2-like superfamily	GENE3D	3.90.730.10		coord: 120..185 e-value: 2.0E-9 score: 39.8
IPR036430	Ribonuclease T2-like superfamily	SUPERFAMILY	55895	Ribonuclease Rh-like	coord: 20..184
IPR001568	Ribonuclease T2-like	PFAM	PF00445	Ribonuclease_T2	coord: 22..116 e-value: 1.5E-20 score: 74.1
IPR001568	Ribonuclease T2-like	PANTHER	PTHR11240	RIBONUCLEASE T2	coord: 119..180
IPR001568	Ribonuclease T2-like	PANTHER	PTHR11240	RIBONUCLEASE T2	coord: 10..114
None	No IPR available	PANTHER	PTHR11240:SF49	RIBONUCLEASE 3	coord: 119..180
None	No IPR available	PANTHER	PTHR11240:SF49	RIBONUCLEASE 3	coord: 10..114
IPR033130	Ribonuclease T2, His active site 2	PROSITE	PS00531	RNASE_T2_2	coord: 102..113
IPR018188	Ribonuclease T2, His active site 1	PROSITE	PS00530	RNASE_T2_1	coord: 52..59

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
PI0023939.1	PI0023939.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016070	RNA metabolic process
cellular_component	GO:0110165	cellular anatomical entity
molecular_function	GO:0033897	ribonuclease T2 activity
molecular_function	GO:0003723	RNA binding