PI0022432 (gene) Melon (PI 482460) v1

Overview
NamePI0022432
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
Descriptionmethionine adenosyltransferase 2 subunit beta
Locationchr11: 25455 .. 28276 (-)
RNA-Seq ExpressionPI0022432
SyntenyPI0022432
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GTCCACTCGTGTGACAGCCACTTATGTTAGAATCTTTTATAAGTCGAGAACCTACGTATTTGTCCACTCACATCAATTAACCCGAATTGAGGGAGCTTCATTTTTCACTTTTCACTTGCGAAGAACCAGAAAGCGATTTGGCAACAAATATGAGGAAGAAGAAGGTTGTGGTAGTGGGCGGCACAGGTTACTTAGGTCAGCATCTTCTATCAGGATTTTCCGAGATCGATGGAGTTCCATATGATATCGCGTTCACTTATAACTCCTTTGCGCCTGAAGATTTGTTGGGAGCTCTTCCTCATCTACTTGCATTTCAAGTTGATTTAAAATCTGGTCAAGGATTCGATACCATTGCTGAGAATTTTGGCCAGGTTTGCCTCTGCCTTCGCCCATTGATGATGTAGAGTGGTTGATTCTTGTAGCTTCAACTTAATTAAGATTTCAATTACCAGCCAGATATTGTTGTTAATTGTGCTGCTATCTCTGTTCCTCGTGCCTGCGAAATAGACCCTGTTTCTGCATTTTCTGTCAACGTGCCTACCGCCCTTGGAACTTGGCTCCTCAGTTTTGAAGGGAGGAATACCCTATTCATTCATCTATCAACTGATCAAGGTAACTTTTTGGTTTCTTTCTTATCCATCATTTTATTTACTATTTGCTTCCATCTTGTTCACTTCAGTTTCCATTACATATATAATGTTGCCTTTGTCTGTTATATATCAACTGGGTACTGCACTATGTTATTTTCCCACCAGTTTATGAAGGCGTGAAATCATTCTATAATGAAGAAGATGAAACCATTCCTGTGAATGTTTATGGCAAGTCAAAGCTAGCAGCTGAACAGTATGTTTCTGAGAAATTTTCTAACTTTGCAATCTTGAGATCCAGTATCATTTTTGGTCCTCAGACCATCTCACCTGTTCCTAAATCTCTTCCTGTTCAGGTTCGTCGTTATGCTTATGCGTGTTATATCGTATTTCTGTAATTCCCTGATACATTAATGCGTTTATACACATATTGGATTGTAAAGAAAAGTATGCAATTAAATTTGGCTATTTTAAAGATATCTAGTGGAAGGGATTTGTGTCATCGTAATATTTTAATAAACATTAACTTATATAACCAACTAACTCAATCGTATCATTTGTTTCTACAGTTCAATAATCTTGTGATAGCCTTCTCGACTTATTTGTACATAACAAGTAATTTGTATGCATTTGATACAGTGGATTGATGCTACCTTGTCTGAAGGAAGTGAAGTTGAATTCTTTCATGATGAGTTTCGATGTCCTGTGTATGTCAAGGATGTCGTGAATGTCATCATAACATTGATTAAGACATGGATATCAGGTATGGATTTTGTAAAGTCTTGATAAGTGGTGCTCCCGAGGTCCATTTCAGTTAAATCCTTTCTTGCTGAATAACTAATTGTAGAATAAAAAACACGGTACATATAATGTATAGTTCACCATTATTAGTCATTTTCCTATGAAGTGATGGAGGAACCACCTCTTTTGAAATTGTTTTATTTTGTTCATTCTGTTGGCATATACTTGCATAATTGATGGTTGTTCAATCATCTTTCTCTTCAGTTGGGGAATGTTGTATTTACCTTTATTATATTAATTTAGTTTCTTATTTGTTTGCGATTATGAATGACTGAATTCCAGAGGGCAAGCAAATGCAATTGCTGTTAAATGTTGGTGGACCAAATCGTGTATCACGTGTTGAAATGGCTGAGACTGTTGCAGAAATTAGAGGGCACAAAAAATCATTGATCAAAAGAGTGTCTGCATCATCAGTATGTTCGCCTCTTGCTCTTTTATAGATCTTTCAAATAGTCATTAAAAGCAGTATTTCTTTCATCATAGACCAAATCATGATTGTTGAATCCACTCGTTCATTTTCACCTCTTTAGAAATATTTCTCGATGGTACTTCTTTCTGACACTAGCCTGTCACTTGGACACGCTTTCTGCTTATCAATCATACAATTCGTGATATGTCTGGTAACAGGCTCCTTCCTCTTGTTGAACTCTCAAGGGAACTGACAAGTTCTCCTCTGGTCTATTGGGTGACATCGTACAAAATGAAAACTTGGAATCCCAAATGGATGCTTGTGGTAATGGCTAAAAAATGTTTGGAGATACCTCCTCCAAGAGCACGAGTTCAAATTCTTAGGCTGAGATTTTAATACCTTAAATTTTGATTGTATCTCGAAGCTTATTATAAGGTGGCAAAAGAATCAGGGACCGGGAGCACAATACAAGAAGTAGAGTTGCAAATAATCCATTTTTCTTTTTAATAAAATGTATATATATATTCTACTTGAATACTCTGTTTGATACAGTGTCTGCCCTTTTAGTAGGCTAAATAGATTGCACAGATATTTTGGTAGCTGTTTATTTCTCCCAGAAGATTGTTTTAATATATCCACTTTTGATTTGTGTTTTCACTTGAATGCTTCAGATCGATCGAGGTGTAAAATCCCCAGCAGACATTTCAATGAACATTAGTAAACTGATCCAGATACTTGCCATGTCTCCTGTTCCATTCACCGACGGTGTCAGATTGACAATCGCAGCCGAGATTACAGAGTGATTTGCATTGGACACTGTAATGACTAATGCTTCACTGCGGCTTTATTTTTTAAGGCTCAATGACTCAAATTTGGTTATTAAGGTCTGCAAACTGCTTCAAAATGCTGCAAATTTATTTCCTTTTCTTGGTTTTATATAATGGTTTGCATCTTTTTTCGTTTTCCAATCATCAACAATTATTTCGTTCTCCTTGTCTTGGAACTTCTAAGAATTTCTGTTCTTC

mRNA sequence

GTCCACTCGTGTGACAGCCACTTATGTTAGAATCTTTTATAAGTCGAGAACCTACGTATTTGTCCACTCACATCAATTAACCCGAATTGAGGGAGCTTCATTTTTCACTTTTCACTTGCGAAGAACCAGAAAGCGATTTGGCAACAAATATGAGGAAGAAGAAGGTTGTGGTAGTGGGCGGCACAGGTTACTTAGGTCAGCATCTTCTATCAGGATTTTCCGAGATCGATGGAGTTCCATATGATATCGCGTTCACTTATAACTCCTTTGCGCCTGAAGATTTGTTGGGAGCTCTTCCTCATCTACTTGCATTTCAAGTTGATTTAAAATCTGGTCAAGGATTCGATACCATTGCTGAGAATTTTGGCCAGCCAGATATTGTTGTTAATTGTGCTGCTATCTCTGTTCCTCGTGCCTGCGAAATAGACCCTGTTTCTGCATTTTCTGTCAACGTGCCTACCGCCCTTGGAACTTGGCTCCTCAGTTTTGAAGGGAGGAATACCCTATTCATTCATCTATCAACTGATCAAGTTTATGAAGGCGTGAAATCATTCTATAATGAAGAAGATGAAACCATTCCTGTGAATGTTTATGGCAAGTCAAAGCTAGCAGCTGAACAGTATGTTTCTGAGAAATTTTCTAACTTTGCAATCTTGAGATCCAGTATCATTTTTGGTCCTCAGACCATCTCACCTGTTCCTAAATCTCTTCCTGTTCAGTGGATTGATGCTACCTTGTCTGAAGGAAGTGAAGTTGAATTCTTTCATGATGAGTTTCGATGTCCTGTGTATGTCAAGGATGTCGTGAATGTCATCATAACATTGATTAAGACATGGATATCAGAGGGCAAGCAAATGCAATTGCTGTTAAATGTTGGTGGACCAAATCGTGTATCACGTGTTGAAATGGCTGAGACTGTTGCAGAAATTAGAGGGCACAAAAAATCATTGATCAAAAGAGTGTCTGCATCATCAATCGATCGAGGTGTAAAATCCCCAGCAGACATTTCAATGAACATTAGTAAACTGATCCAGATACTTGCCATGTCTCCTGTTCCATTCACCGACGGTGTCAGATTGACAATCGCAGCCGAGATTACAGAGTGATTTGCATTGGACACTGTAATGACTAATGCTTCACTGCGGCTTTATTTTTTAAGGCTCAATGACTCAAATTTGGTTATTAAGGTCTGCAAACTGCTTCAAAATGCTGCAAATTTATTTCCTTTTCTTGGTTTTATATAATGGTTTGCATCTTTTTTCGTTTTCCAATCATCAACAATTATTTCGTTCTCCTTGTCTTGGAACTTCTAAGAATTTCTGTTCTTC

Coding sequence (CDS)

ATGAGGAAGAAGAAGGTTGTGGTAGTGGGCGGCACAGGTTACTTAGGTCAGCATCTTCTATCAGGATTTTCCGAGATCGATGGAGTTCCATATGATATCGCGTTCACTTATAACTCCTTTGCGCCTGAAGATTTGTTGGGAGCTCTTCCTCATCTACTTGCATTTCAAGTTGATTTAAAATCTGGTCAAGGATTCGATACCATTGCTGAGAATTTTGGCCAGCCAGATATTGTTGTTAATTGTGCTGCTATCTCTGTTCCTCGTGCCTGCGAAATAGACCCTGTTTCTGCATTTTCTGTCAACGTGCCTACCGCCCTTGGAACTTGGCTCCTCAGTTTTGAAGGGAGGAATACCCTATTCATTCATCTATCAACTGATCAAGTTTATGAAGGCGTGAAATCATTCTATAATGAAGAAGATGAAACCATTCCTGTGAATGTTTATGGCAAGTCAAAGCTAGCAGCTGAACAGTATGTTTCTGAGAAATTTTCTAACTTTGCAATCTTGAGATCCAGTATCATTTTTGGTCCTCAGACCATCTCACCTGTTCCTAAATCTCTTCCTGTTCAGTGGATTGATGCTACCTTGTCTGAAGGAAGTGAAGTTGAATTCTTTCATGATGAGTTTCGATGTCCTGTGTATGTCAAGGATGTCGTGAATGTCATCATAACATTGATTAAGACATGGATATCAGAGGGCAAGCAAATGCAATTGCTGTTAAATGTTGGTGGACCAAATCGTGTATCACGTGTTGAAATGGCTGAGACTGTTGCAGAAATTAGAGGGCACAAAAAATCATTGATCAAAAGAGTGTCTGCATCATCAATCGATCGAGGTGTAAAATCCCCAGCAGACATTTCAATGAACATTAGTAAACTGATCCAGATACTTGCCATGTCTCCTGTTCCATTCACCGACGGTGTCAGATTGACAATCGCAGCCGAGATTACAGAGTGA

Protein sequence

MRKKKVVVVGGTGYLGQHLLSGFSEIDGVPYDIAFTYNSFAPEDLLGALPHLLAFQVDLKSGQGFDTIAENFGQPDIVVNCAAISVPRACEIDPVSAFSVNVPTALGTWLLSFEGRNTLFIHLSTDQVYEGVKSFYNEEDETIPVNVYGKSKLAAEQYVSEKFSNFAILRSSIIFGPQTISPVPKSLPVQWIDATLSEGSEVEFFHDEFRCPVYVKDVVNVIITLIKTWISEGKQMQLLLNVGGPNRVSRVEMAETVAEIRGHKKSLIKRVSASSIDRGVKSPADISMNISKLIQILAMSPVPFTDGVRLTIAAEITE
Homology
BLAST of PI0022432 vs. ExPASy Swiss-Prot
Match: D4GU71 (Probable low-salt glycan biosynthesis reductase Agl14 OS=Haloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2) OX=309800 GN=agl14 PE=3 SV=1)

HSP 1 Score: 97.4 bits (241), Expect = 3.0e-19
Identity = 74/260 (28.46%), Postives = 118/260 (45.38%), Query Frame = 0

Query: 56  QVDLKSGQGFDTIAENFGQPDIVVNCAAISVPRACEIDPVSAFSVNVPTALGTWLLSFEG 115
           QVD+   +    + + +   D+V+NCAA +    CE +P  A +VN  TA G      + 
Sbjct: 44  QVDITDTERVVELLDEY-DVDLVINCAAYTDVDGCESNPEVATAVN-GTAPGDLAAVCDD 103

Query: 116 RNTLFIHLSTDQVYEG-VKSFYNEEDETIPVNVYGKSKLAAEQYVSEKFSNFAILRSSII 175
           R   FIH STD V++G    FY E DE  P+  YG+SKL  E  V +   +  ILR S +
Sbjct: 104 REIPFIHYSTDYVFDGETDGFYEEGDEPAPIQEYGRSKLTGEHAVRDVNPDALILRLSFV 163

Query: 176 FGPQTISPVPKSLPVQWIDATLSEGSEVEFFHDEFRCPVYVKDVVNVIITLIKTWISEGK 235
           +G +  +      P QW+ +TL+ G  V  F D+   P    +V    + L+   +S   
Sbjct: 164 YGARGDTSDLVGFP-QWVASTLAAGDTVPLFTDQTMTPSRAGNVATTTLELLDAGVSG-- 223

Query: 236 QMQLLLNVGGPNRVSRVEMAETVAEIRGHKKSLIKRVSASSIDRGVKSPADISMNISKLI 295
                 +V   + V+  +  E + E+ G   +LI+    + +DR    P    +++S + 
Sbjct: 224 ----TFHVASQSAVTPSDFGEKICEVIGGDATLIESSVMADLDRPAARPRRSCLDVSNVE 283

Query: 296 QILAMSPVPFTDGVRLTIAA 315
             L  S     D +    AA
Sbjct: 284 GELGCSQPTLEDDLAALEAA 294

BLAST of PI0022432 vs. ExPASy Swiss-Prot
Match: Q99LB6 (Methionine adenosyltransferase 2 subunit beta OS=Mus musculus OX=10090 GN=Mat2b PE=1 SV=1)

HSP 1 Score: 90.9 bits (224), Expect = 2.8e-17
Identity = 75/306 (24.51%), Postives = 139/306 (45.42%), Query Frame = 0

Query: 4   KKVVVVGGTGYLGQHLLSGFSEIDGVPYDIAFTYNSFAPEDLLGALPHLLAFQVDLKSGQ 63
           ++V++ G TG LG+ +   F + +   + +   +    P+            QV+L   +
Sbjct: 29  RRVLITGATGLLGRAVYKEFQQSNW--HTVGCGFRRARPK----------FEQVNLLDSE 88

Query: 64  GFDTIAENFGQPDIVVNCAAISVPRACEIDPVSAFSVNVPTALGTWLLSFEGRNTLFIHL 123
               +  +F QP ++V+CAA   P   E  P +A  +NV  A G             I++
Sbjct: 89  AVHHLIHDF-QPHVIVHCAAERRPDVVESQPDAASQLNV-GASGNLAKEAAAIGAFLIYI 148

Query: 124 STDQVYEGVKSFYNEEDETIPVNVYGKSKLAAEQYVSEKFSNFAILRSSIIFGPQTISPV 183
           S+D V++G    Y EED   P+N+YGK+KL  E+ V E     A+LR  +++G   +  +
Sbjct: 149 SSDYVFDGTNPPYTEEDIPSPLNLYGKTKLDGEKAVLENNLGAAVLRIPVLYG--EVEKL 208

Query: 184 PKSLPVQWIDATLSEGSEVEFFHDEFRCPVYVKDVVNVIITLIKTWISEGKQMQLLLNVG 243
            +S      D            H + R P +VKDV +V   L +  + +   ++   +  
Sbjct: 209 EESAVTVMFDKVQFSNKSANMDHWQQRFPTHVKDVASVCRQLAEKRMLD-PSIKGTFHWS 268

Query: 244 GPNRVSRVEMAETVAEIRGHKKSLIKRVSASSIDRGVKSPADISMNISKLIQILAMSPVP 303
           G  ++++ EMA  +A+      S ++ ++ S +  G + P +  ++ SKL  +      P
Sbjct: 269 GNEQMTKYEMACAIADAFNLPSSHLRPITDSPV-IGAQRPKNAQLDCSKLETLGIGQRTP 316

Query: 304 FTDGVR 310
           F  G++
Sbjct: 329 FRTGIK 316

BLAST of PI0022432 vs. ExPASy Swiss-Prot
Match: Q9NZL9 (Methionine adenosyltransferase 2 subunit beta OS=Homo sapiens OX=9606 GN=MAT2B PE=1 SV=1)

HSP 1 Score: 89.7 bits (221), Expect = 6.3e-17
Identity = 78/306 (25.49%), Postives = 137/306 (44.77%), Query Frame = 0

Query: 4   KKVVVVGGTGYLGQHLLSGFSEIDGVPYDIAFTYNSFAPEDLLGALPHLLAFQVDLKSGQ 63
           ++V+V G TG LG+ +   F + +   + +   +    P+            QV+L    
Sbjct: 29  RRVLVTGATGLLGRAVHKEFQQNNW--HAVGCGFRRARPK----------FEQVNLLDSN 88

Query: 64  GFDTIAENFGQPDIVVNCAAISVPRACEIDPVSAFSVNVPTALGTWLLSFEGRNTLFIHL 123
               I  +F QP ++V+CAA   P   E  P +A  +NV  A G             I++
Sbjct: 89  AVHHIIHDF-QPHVIVHCAAERRPDVVENQPDAASQLNV-DASGNLAKEAAAVGAFLIYI 148

Query: 124 STDQVYEGVKSFYNEEDETIPVNVYGKSKLAAEQYVSEKFSNFAILRSSIIFGPQTISPV 183
           S+D V++G    Y EED   P+N+YGK+KL  E+ V E     A+LR  I++G   +  +
Sbjct: 149 SSDYVFDGTNPPYREEDIPAPLNLYGKTKLDGEKAVLENNLGAAVLRIPILYG--EVEKL 208

Query: 184 PKSLPVQWIDATLSEGSEVEFFHDEFRCPVYVKDVVNVIITLIKTWISEGKQMQLLLNVG 243
            +S      D            H + R P +VKDV  V   L +  + +   ++   +  
Sbjct: 209 EESAVTVMFDKVQFSNKSANMDHWQQRFPTHVKDVATVCRQLAEKRMLD-PSIKGTFHWS 268

Query: 244 GPNRVSRVEMAETVAEIRGHKKSLIKRVSASSIDRGVKSPADISMNISKLIQILAMSPVP 303
           G  ++++ EMA  +A+      S ++ ++ S +  G + P +  ++ SKL  +      P
Sbjct: 269 GNEQMTKYEMACAIADAFNLPSSHLRPITDSPV-LGAQRPRNAQLDCSKLETLGIGQRTP 316

Query: 304 FTDGVR 310
           F  G++
Sbjct: 329 FRIGIK 316

BLAST of PI0022432 vs. ExPASy Swiss-Prot
Match: Q5R4E0 (Methionine adenosyltransferase 2 subunit beta OS=Pongo abelii OX=9601 GN=MAT2B PE=2 SV=1)

HSP 1 Score: 89.7 bits (221), Expect = 6.3e-17
Identity = 78/306 (25.49%), Postives = 137/306 (44.77%), Query Frame = 0

Query: 4   KKVVVVGGTGYLGQHLLSGFSEIDGVPYDIAFTYNSFAPEDLLGALPHLLAFQVDLKSGQ 63
           ++V+V G TG LG+ +   F + +   + +   +    P+            QV+L    
Sbjct: 29  RRVLVTGATGLLGRAVHKEFQQNNW--HAVGCGFRRARPK----------FEQVNLLDSN 88

Query: 64  GFDTIAENFGQPDIVVNCAAISVPRACEIDPVSAFSVNVPTALGTWLLSFEGRNTLFIHL 123
               I  +F QP ++V+CAA   P   E  P +A  +NV  A G             I++
Sbjct: 89  AVHHIIHDF-QPHVIVHCAAERRPDVVENQPDAASQLNV-DASGNLAKEAAAVGAFLIYI 148

Query: 124 STDQVYEGVKSFYNEEDETIPVNVYGKSKLAAEQYVSEKFSNFAILRSSIIFGPQTISPV 183
           S+D V++G    Y EED   P+N+YGK+KL  E+ V E     A+LR  I++G   +  +
Sbjct: 149 SSDYVFDGTNPPYREEDIPAPLNLYGKTKLDGEKAVLENNLGAAVLRIPILYG--EVEKL 208

Query: 184 PKSLPVQWIDATLSEGSEVEFFHDEFRCPVYVKDVVNVIITLIKTWISEGKQMQLLLNVG 243
            +S      D            H + R P +VKDV  V   L +  + +   ++   +  
Sbjct: 209 EESAVTVMFDKVQFSNKSANMDHWQQRFPTHVKDVATVCRQLAEKRMLD-PSIKGTFHWS 268

Query: 244 GPNRVSRVEMAETVAEIRGHKKSLIKRVSASSIDRGVKSPADISMNISKLIQILAMSPVP 303
           G  ++++ EMA  +A+      S ++ ++ S +  G + P +  ++ SKL  +      P
Sbjct: 269 GNEQMTKYEMACAIADAFNLPSSHLRPITDSPV-LGAQRPRNAQLDCSKLETLGIGQRTP 316

Query: 304 FTDGVR 310
           F  G++
Sbjct: 329 FRIGIK 316

BLAST of PI0022432 vs. ExPASy Swiss-Prot
Match: Q5U2R0 (Methionine adenosyltransferase 2 subunit beta OS=Rattus norvegicus OX=10116 GN=Mat2b PE=2 SV=1)

HSP 1 Score: 89.7 bits (221), Expect = 6.3e-17
Identity = 75/306 (24.51%), Postives = 139/306 (45.42%), Query Frame = 0

Query: 4   KKVVVVGGTGYLGQHLLSGFSEIDGVPYDIAFTYNSFAPEDLLGALPHLLAFQVDLKSGQ 63
           ++V++ G TG LG+ +   F + +   + +   +    P+            QV+L   +
Sbjct: 29  RRVLITGATGLLGRAVYKEFQQSNW--HAVGCGFRRARPK----------FEQVNLLDSE 88

Query: 64  GFDTIAENFGQPDIVVNCAAISVPRACEIDPVSAFSVNVPTALGTWLLSFEGRNTLFIHL 123
               +  +F QP ++V+CAA   P   E  P +A  +NV  A G             I++
Sbjct: 89  AVHHLIHDF-QPHVIVHCAAERRPDVVESQPDAASQLNV-GASGNLAKEAAAIGAFLIYI 148

Query: 124 STDQVYEGVKSFYNEEDETIPVNVYGKSKLAAEQYVSEKFSNFAILRSSIIFGPQTISPV 183
           S+D V++G    Y EED   P+N+YGK+KL  E+ V E     A+LR  +++G   +  +
Sbjct: 149 SSDYVFDGTNPPYTEEDIPSPLNLYGKTKLDGEKAVLENNLGAAVLRIPVLYG--EVEKL 208

Query: 184 PKSLPVQWIDATLSEGSEVEFFHDEFRCPVYVKDVVNVIITLIKTWISEGKQMQLLLNVG 243
            +S      D            H + R P +VKDV +V   L +  + +   ++   +  
Sbjct: 209 EESAVTVMFDKVQFSNKSANMDHWQQRFPTHVKDVASVCRQLAEKRMLD-PSIKGTFHWS 268

Query: 244 GPNRVSRVEMAETVAEIRGHKKSLIKRVSASSIDRGVKSPADISMNISKLIQILAMSPVP 303
           G  ++++ EMA  +A+      S ++ ++ S +  G + P +  ++ SKL  +      P
Sbjct: 269 GNEQMTKYEMACAIADAFNLPSSHLRPITDSPV-IGAQRPKNAQLDCSKLETLGIGQRTP 316

Query: 304 FTDGVR 310
           F  G++
Sbjct: 329 FRIGIK 316

BLAST of PI0022432 vs. ExPASy TrEMBL
Match: A0A5D3DLR1 (Putative dTDP-4-dehydrorhamnose reductase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold266G00020 PE=4 SV=1)

HSP 1 Score: 609.4 bits (1570), Expect = 8.7e-171
Identity = 307/318 (96.54%), Postives = 313/318 (98.43%), Query Frame = 0

Query: 1   MRKKKVVVVGGTGYLGQHLLSGFSEIDGVPYDIAFTYNSFAPEDLLGALPHLLAFQVDLK 60
           MRKKKV+VVGGTGYLGQHLLSG SEIDGVPYDIAFTY+SFAPEDLLGALPHLLAFQVDLK
Sbjct: 1   MRKKKVLVVGGTGYLGQHLLSGLSEIDGVPYDIAFTYHSFAPEDLLGALPHLLAFQVDLK 60

Query: 61  SGQGFDTIAENFGQPDIVVNCAAISVPRACEIDPVSAFSVNVPTALGTWLLSFEGRNTLF 120
           SGQGFDT+AENFGQPDIVVNCAAISVPRACEIDPVSAFSVNVPTALGTWLLSFEGRNTLF
Sbjct: 61  SGQGFDTVAENFGQPDIVVNCAAISVPRACEIDPVSAFSVNVPTALGTWLLSFEGRNTLF 120

Query: 121 IHLSTDQVYEGVKSFYNEEDETIPVNVYGKSKLAAEQYVSEKFSNFAILRSSIIFGPQTI 180
           IHLSTDQVYEGVKSFYNEE+ETIPVNVYGKSKLAAEQYVSEKFSNFAILRSSIIFGPQTI
Sbjct: 121 IHLSTDQVYEGVKSFYNEENETIPVNVYGKSKLAAEQYVSEKFSNFAILRSSIIFGPQTI 180

Query: 181 SPVPKSLPVQWIDATLSEGSEVEFFHDEFRCPVYVKDVVNVIITLIKTWISEGKQMQLLL 240
           SPVPKSLPVQWIDATLSEG EVEFFHDEFRCPVYVKDVVNVIITLIKTWISEGKQMQLLL
Sbjct: 181 SPVPKSLPVQWIDATLSEGREVEFFHDEFRCPVYVKDVVNVIITLIKTWISEGKQMQLLL 240

Query: 241 NVGGPNRVSRVEMAETVAEIRGHKKSLIKRVSASSIDRGVKSPADISMNISKLIQILAMS 300
           NVGGPNRVSRVEMAETVAEIRGHKKSLIKRVSASSIDRGVKSPADISMNI KL+QILAM 
Sbjct: 241 NVGGPNRVSRVEMAETVAEIRGHKKSLIKRVSASSIDRGVKSPADISMNIDKLVQILAMF 300

Query: 301 PVPFTDGVRLTIAAEITE 319
           PV FTDGVRLT+AAEITE
Sbjct: 301 PVSFTDGVRLTLAAEITE 318

BLAST of PI0022432 vs. ExPASy TrEMBL
Match: A0A1S3CD69 (probable dTDP-4-dehydrorhamnose reductase OS=Cucumis melo OX=3656 GN=LOC103499064 PE=4 SV=1)

HSP 1 Score: 609.4 bits (1570), Expect = 8.7e-171
Identity = 307/318 (96.54%), Postives = 313/318 (98.43%), Query Frame = 0

Query: 1   MRKKKVVVVGGTGYLGQHLLSGFSEIDGVPYDIAFTYNSFAPEDLLGALPHLLAFQVDLK 60
           MRKKKV+VVGGTGYLGQHLLSG SEIDGVPYDIAFTY+SFAPEDLLGALPHLLAFQVDLK
Sbjct: 1   MRKKKVLVVGGTGYLGQHLLSGLSEIDGVPYDIAFTYHSFAPEDLLGALPHLLAFQVDLK 60

Query: 61  SGQGFDTIAENFGQPDIVVNCAAISVPRACEIDPVSAFSVNVPTALGTWLLSFEGRNTLF 120
           SGQGFDT+AENFGQPDIVVNCAAISVPRACEIDPVSAFSVNVPTALGTWLLSFEGRNTLF
Sbjct: 61  SGQGFDTVAENFGQPDIVVNCAAISVPRACEIDPVSAFSVNVPTALGTWLLSFEGRNTLF 120

Query: 121 IHLSTDQVYEGVKSFYNEEDETIPVNVYGKSKLAAEQYVSEKFSNFAILRSSIIFGPQTI 180
           IHLSTDQVYEGVKSFYNEE+ETIPVNVYGKSKLAAEQYVSEKFSNFAILRSSIIFGPQTI
Sbjct: 121 IHLSTDQVYEGVKSFYNEENETIPVNVYGKSKLAAEQYVSEKFSNFAILRSSIIFGPQTI 180

Query: 181 SPVPKSLPVQWIDATLSEGSEVEFFHDEFRCPVYVKDVVNVIITLIKTWISEGKQMQLLL 240
           SPVPKSLPVQWIDATLSEG EVEFFHDEFRCPVYVKDVVNVIITLIKTWISEGKQMQLLL
Sbjct: 181 SPVPKSLPVQWIDATLSEGREVEFFHDEFRCPVYVKDVVNVIITLIKTWISEGKQMQLLL 240

Query: 241 NVGGPNRVSRVEMAETVAEIRGHKKSLIKRVSASSIDRGVKSPADISMNISKLIQILAMS 300
           NVGGPNRVSRVEMAETVAEIRGHKKSLIKRVSASSIDRGVKSPADISMNI KL+QILAM 
Sbjct: 241 NVGGPNRVSRVEMAETVAEIRGHKKSLIKRVSASSIDRGVKSPADISMNIDKLVQILAMF 300

Query: 301 PVPFTDGVRLTIAAEITE 319
           PV FTDGVRLT+AAEITE
Sbjct: 301 PVSFTDGVRLTLAAEITE 318

BLAST of PI0022432 vs. ExPASy TrEMBL
Match: A0A0A0KEJ7 (RmlD_sub_bind domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G074000 PE=4 SV=1)

HSP 1 Score: 599.0 bits (1543), Expect = 1.2e-167
Identity = 300/314 (95.54%), Postives = 309/314 (98.41%), Query Frame = 0

Query: 1   MRKKKVVVVGGTGYLGQHLLSGFSEIDGVPYDIAFTYNSFAPEDLLGALPHLLAFQVDLK 60
           MRKKKV+VVGGTGYLGQHLLSGFSEIDGVPYDIAFTY+SFAPE LLGALPHLL+FQVDLK
Sbjct: 1   MRKKKVLVVGGTGYLGQHLLSGFSEIDGVPYDIAFTYHSFAPEALLGALPHLLSFQVDLK 60

Query: 61  SGQGFDTIAENFGQPDIVVNCAAISVPRACEIDPVSAFSVNVPTALGTWLLSFEGRNTLF 120
           SGQGFDTI ENFGQPDIVVNCAAISVPRACE+DPVSAFSVNVPTA+GTWLLSFEGRNTLF
Sbjct: 61  SGQGFDTITENFGQPDIVVNCAAISVPRACEVDPVSAFSVNVPTAIGTWLLSFEGRNTLF 120

Query: 121 IHLSTDQVYEGVKSFYNEEDETIPVNVYGKSKLAAEQYVSEKFSNFAILRSSIIFGPQTI 180
           IHLSTDQVYEGV+SFYNEEDETIPVNVYGKSKLAAEQYVSEKFSNFAILRSSIIFGPQTI
Sbjct: 121 IHLSTDQVYEGVESFYNEEDETIPVNVYGKSKLAAEQYVSEKFSNFAILRSSIIFGPQTI 180

Query: 181 SPVPKSLPVQWIDATLSEGSEVEFFHDEFRCPVYVKDVVNVIITLIKTWISEGKQMQLLL 240
           SPVPKSLPVQWIDATLSEG+EVEFFHDEFRCPVYVKDVVNVIITLIKTWISEGKQMQLLL
Sbjct: 181 SPVPKSLPVQWIDATLSEGNEVEFFHDEFRCPVYVKDVVNVIITLIKTWISEGKQMQLLL 240

Query: 241 NVGGPNRVSRVEMAETVAEIRGHKKSLIKRVSASSIDRGVKSPADISMNISKLIQILAMS 300
           NVGGPNRVSRVEMAETVAEIRGHKKSLIKRVSASSIDRGVKSPADISMNI KLIQILAMS
Sbjct: 241 NVGGPNRVSRVEMAETVAEIRGHKKSLIKRVSASSIDRGVKSPADISMNIGKLIQILAMS 300

Query: 301 PVPFTDGVRLTIAA 315
           PV FTDGVRLT+ +
Sbjct: 301 PVSFTDGVRLTLCS 314

BLAST of PI0022432 vs. ExPASy TrEMBL
Match: A0A6J1KBW1 (uncharacterized protein LOC111494187 OS=Cucurbita maxima OX=3661 GN=LOC111494187 PE=4 SV=1)

HSP 1 Score: 552.0 bits (1421), Expect = 1.6e-153
Identity = 274/318 (86.16%), Postives = 296/318 (93.08%), Query Frame = 0

Query: 1   MRKKKVVVVGGTGYLGQHLLSGFSEIDGVPYDIAFTYNSFAPEDLLGALPHLLAFQVDLK 60
           M KKKV+V+GGTGYLGQHLLSGFS+IDGVPYD+AFTY+SFAP+DL+ ALPHLLAFQVDLK
Sbjct: 1   MSKKKVLVLGGTGYLGQHLLSGFSDIDGVPYDLAFTYHSFAPDDLVRALPHLLAFQVDLK 60

Query: 61  SGQGFDTIAENFGQPDIVVNCAAISVPRACEIDPVSAFSVNVPTALGTWLLSFEGRNTLF 120
           SGQGFD I + FGQPDIVVNCAA+SVPRACE DP SA S+NVPTALG WLLSFE  NTL 
Sbjct: 61  SGQGFDAIVQKFGQPDIVVNCAAVSVPRACETDPASALSINVPTALGNWLLSFEQMNTLL 120

Query: 121 IHLSTDQVYEGVKSFYNEEDETIPVNVYGKSKLAAEQYVSEKFSNFAILRSSIIFGPQTI 180
           IHLSTDQVYEG++SFY E+DETIPVNVYGKSKLAAEQYVSEKFSNFAILRSSIIFGPQTI
Sbjct: 121 IHLSTDQVYEGMRSFYKEDDETIPVNVYGKSKLAAEQYVSEKFSNFAILRSSIIFGPQTI 180

Query: 181 SPVPKSLPVQWIDATLSEGSEVEFFHDEFRCPVYVKDVVNVIITLIKTWISEGKQMQLLL 240
           SPV KSLPVQWIDA LS+G+EVEFFHDEFRCPVYVKDVV+VIITLIKTWISEGKQMQL+L
Sbjct: 181 SPVHKSLPVQWIDAALSKGNEVEFFHDEFRCPVYVKDVVSVIITLIKTWISEGKQMQLVL 240

Query: 241 NVGGPNRVSRVEMAETVAEIRGHKKSLIKRVSASSIDRGVKSPADISMNISKLIQILAMS 300
           NVGGPNR+SRVEMAETVAEIRGH KSLIKRVSASSIDRGVKSPADISMNIS L+Q LA+S
Sbjct: 241 NVGGPNRLSRVEMAETVAEIRGHNKSLIKRVSASSIDRGVKSPADISMNISNLVQKLAIS 300

Query: 301 PVPFTDGVRLTIAAEITE 319
           PV F +GVRLT+AAE  E
Sbjct: 301 PVSFNEGVRLTLAAETKE 318

BLAST of PI0022432 vs. ExPASy TrEMBL
Match: A0A6J1DR99 (methionine adenosyltransferase 2 subunit beta OS=Momordica charantia OX=3673 GN=LOC111023110 PE=4 SV=1)

HSP 1 Score: 548.1 bits (1411), Expect = 2.4e-152
Identity = 274/315 (86.98%), Postives = 291/315 (92.38%), Query Frame = 0

Query: 1   MRKKKVVVVGGTGYLGQHLLSGFSEIDGVPYDIAFTYNSFAPEDLLGALPHLLAFQVDLK 60
           M +KKV+VVGGTGYLGQHLLSGFS I+G PYDIAFTY+SF PEDLL ALP+LLAF VDLK
Sbjct: 1   MSRKKVLVVGGTGYLGQHLLSGFSGIEGAPYDIAFTYHSFPPEDLLRALPNLLAFHVDLK 60

Query: 61  SGQGFDTIAENFGQPDIVVNCAAISVPRACEIDPVSAFSVNVPTALGTWLLSFEGRNTLF 120
           SGQGFD IAE FGQPD+VVNCAAISVPRACE DP SA S+NVPTALG W+LSFE RNTL 
Sbjct: 61  SGQGFDAIAEKFGQPDVVVNCAAISVPRACETDPASALSINVPTALGNWMLSFEERNTLL 120

Query: 121 IHLSTDQVYEGVKSFYNEEDETIPVNVYGKSKLAAEQYVSEKFSNFAILRSSIIFGPQTI 180
           +HLSTDQVYEGV+SFY EEDETIPVNVYGKSKLAAEQYVSEKF NFAILRSSIIFGPQTI
Sbjct: 121 VHLSTDQVYEGVRSFYKEEDETIPVNVYGKSKLAAEQYVSEKFPNFAILRSSIIFGPQTI 180

Query: 181 SPVPKSLPVQWIDATLSEGSEVEFFHDEFRCPVYVKDVVNVIITLIKTWISEGKQMQLLL 240
           SPVPKSLP+QWIDA L++GSEV+FFHDEFRCPVYVKDVV VII LIKTWISE KQMQLLL
Sbjct: 181 SPVPKSLPIQWIDAVLTKGSEVDFFHDEFRCPVYVKDVVAVIIKLIKTWISESKQMQLLL 240

Query: 241 NVGGPNRVSRVEMAETVAEIRGHKKSLIKRVSASSIDRGVKSPADISMNISKLIQILAMS 300
           NVGGPNRVSRVEMAETVAEIRGH KSLIKRVSASSIDRGVKSPADISMNISKL+Q LA+S
Sbjct: 241 NVGGPNRVSRVEMAETVAEIRGHNKSLIKRVSASSIDRGVKSPADISMNISKLVQKLAIS 300

Query: 301 PVPFTDGVRLTIAAE 316
           PV FT GVRLT+AAE
Sbjct: 301 PVSFTHGVRLTLAAE 315

BLAST of PI0022432 vs. NCBI nr
Match: XP_008460177.1 (PREDICTED: probable dTDP-4-dehydrorhamnose reductase [Cucumis melo] >TYK24462.1 putative dTDP-4-dehydrorhamnose reductase [Cucumis melo var. makuwa])

HSP 1 Score: 609.4 bits (1570), Expect = 1.8e-170
Identity = 307/318 (96.54%), Postives = 313/318 (98.43%), Query Frame = 0

Query: 1   MRKKKVVVVGGTGYLGQHLLSGFSEIDGVPYDIAFTYNSFAPEDLLGALPHLLAFQVDLK 60
           MRKKKV+VVGGTGYLGQHLLSG SEIDGVPYDIAFTY+SFAPEDLLGALPHLLAFQVDLK
Sbjct: 1   MRKKKVLVVGGTGYLGQHLLSGLSEIDGVPYDIAFTYHSFAPEDLLGALPHLLAFQVDLK 60

Query: 61  SGQGFDTIAENFGQPDIVVNCAAISVPRACEIDPVSAFSVNVPTALGTWLLSFEGRNTLF 120
           SGQGFDT+AENFGQPDIVVNCAAISVPRACEIDPVSAFSVNVPTALGTWLLSFEGRNTLF
Sbjct: 61  SGQGFDTVAENFGQPDIVVNCAAISVPRACEIDPVSAFSVNVPTALGTWLLSFEGRNTLF 120

Query: 121 IHLSTDQVYEGVKSFYNEEDETIPVNVYGKSKLAAEQYVSEKFSNFAILRSSIIFGPQTI 180
           IHLSTDQVYEGVKSFYNEE+ETIPVNVYGKSKLAAEQYVSEKFSNFAILRSSIIFGPQTI
Sbjct: 121 IHLSTDQVYEGVKSFYNEENETIPVNVYGKSKLAAEQYVSEKFSNFAILRSSIIFGPQTI 180

Query: 181 SPVPKSLPVQWIDATLSEGSEVEFFHDEFRCPVYVKDVVNVIITLIKTWISEGKQMQLLL 240
           SPVPKSLPVQWIDATLSEG EVEFFHDEFRCPVYVKDVVNVIITLIKTWISEGKQMQLLL
Sbjct: 181 SPVPKSLPVQWIDATLSEGREVEFFHDEFRCPVYVKDVVNVIITLIKTWISEGKQMQLLL 240

Query: 241 NVGGPNRVSRVEMAETVAEIRGHKKSLIKRVSASSIDRGVKSPADISMNISKLIQILAMS 300
           NVGGPNRVSRVEMAETVAEIRGHKKSLIKRVSASSIDRGVKSPADISMNI KL+QILAM 
Sbjct: 241 NVGGPNRVSRVEMAETVAEIRGHKKSLIKRVSASSIDRGVKSPADISMNIDKLVQILAMF 300

Query: 301 PVPFTDGVRLTIAAEITE 319
           PV FTDGVRLT+AAEITE
Sbjct: 301 PVSFTDGVRLTLAAEITE 318

BLAST of PI0022432 vs. NCBI nr
Match: XP_004144979.1 (uncharacterized protein LOC101221549 [Cucumis sativus] >KGN46196.1 hypothetical protein Csa_004988 [Cucumis sativus])

HSP 1 Score: 599.0 bits (1543), Expect = 2.4e-167
Identity = 300/314 (95.54%), Postives = 309/314 (98.41%), Query Frame = 0

Query: 1   MRKKKVVVVGGTGYLGQHLLSGFSEIDGVPYDIAFTYNSFAPEDLLGALPHLLAFQVDLK 60
           MRKKKV+VVGGTGYLGQHLLSGFSEIDGVPYDIAFTY+SFAPE LLGALPHLL+FQVDLK
Sbjct: 1   MRKKKVLVVGGTGYLGQHLLSGFSEIDGVPYDIAFTYHSFAPEALLGALPHLLSFQVDLK 60

Query: 61  SGQGFDTIAENFGQPDIVVNCAAISVPRACEIDPVSAFSVNVPTALGTWLLSFEGRNTLF 120
           SGQGFDTI ENFGQPDIVVNCAAISVPRACE+DPVSAFSVNVPTA+GTWLLSFEGRNTLF
Sbjct: 61  SGQGFDTITENFGQPDIVVNCAAISVPRACEVDPVSAFSVNVPTAIGTWLLSFEGRNTLF 120

Query: 121 IHLSTDQVYEGVKSFYNEEDETIPVNVYGKSKLAAEQYVSEKFSNFAILRSSIIFGPQTI 180
           IHLSTDQVYEGV+SFYNEEDETIPVNVYGKSKLAAEQYVSEKFSNFAILRSSIIFGPQTI
Sbjct: 121 IHLSTDQVYEGVESFYNEEDETIPVNVYGKSKLAAEQYVSEKFSNFAILRSSIIFGPQTI 180

Query: 181 SPVPKSLPVQWIDATLSEGSEVEFFHDEFRCPVYVKDVVNVIITLIKTWISEGKQMQLLL 240
           SPVPKSLPVQWIDATLSEG+EVEFFHDEFRCPVYVKDVVNVIITLIKTWISEGKQMQLLL
Sbjct: 181 SPVPKSLPVQWIDATLSEGNEVEFFHDEFRCPVYVKDVVNVIITLIKTWISEGKQMQLLL 240

Query: 241 NVGGPNRVSRVEMAETVAEIRGHKKSLIKRVSASSIDRGVKSPADISMNISKLIQILAMS 300
           NVGGPNRVSRVEMAETVAEIRGHKKSLIKRVSASSIDRGVKSPADISMNI KLIQILAMS
Sbjct: 241 NVGGPNRVSRVEMAETVAEIRGHKKSLIKRVSASSIDRGVKSPADISMNIGKLIQILAMS 300

Query: 301 PVPFTDGVRLTIAA 315
           PV FTDGVRLT+ +
Sbjct: 301 PVSFTDGVRLTLCS 314

BLAST of PI0022432 vs. NCBI nr
Match: XP_038878319.1 (methionine adenosyltransferase 2 subunit beta [Benincasa hispida])

HSP 1 Score: 580.9 bits (1496), Expect = 6.9e-162
Identity = 293/318 (92.14%), Postives = 301/318 (94.65%), Query Frame = 0

Query: 1   MRKKKVVVVGGTGYLGQHLLSGFSEIDGVPYDIAFTYNSFAPEDLLGALPHLLAFQVDLK 60
           M K KV+VVGGTGYLGQHLLSGF E DGVPYDIAFTY+SFAP DLL ALPH LAFQVDLK
Sbjct: 1   MSKNKVLVVGGTGYLGQHLLSGFFESDGVPYDIAFTYHSFAPGDLLRALPHFLAFQVDLK 60

Query: 61  SGQGFDTIAENFGQPDIVVNCAAISVPRACEIDPVSAFSVNVPTALGTWLLSFEGRNTLF 120
           SGQGFDTIAE FGQPDIVVNCAA+SVPRACE DPVSA S+NVPTALG WLLSFEGRNTL 
Sbjct: 61  SGQGFDTIAEKFGQPDIVVNCAAVSVPRACETDPVSALSINVPTALGNWLLSFEGRNTLL 120

Query: 121 IHLSTDQVYEGVKSFYNEEDETIPVNVYGKSKLAAEQYVSEKFSNFAILRSSIIFGPQTI 180
           IHLSTDQVYEGV SFY EEDETIPVNVYGKSKLAAEQYVSEKFSNFAILRSSIIFGPQTI
Sbjct: 121 IHLSTDQVYEGVTSFYKEEDETIPVNVYGKSKLAAEQYVSEKFSNFAILRSSIIFGPQTI 180

Query: 181 SPVPKSLPVQWIDATLSEGSEVEFFHDEFRCPVYVKDVVNVIITLIKTWISEGKQMQLLL 240
           SPVPKSLPVQWIDATLS+GSEVEFFHDEFRCPVYVKDVVNVIITLIKTWISEGKQMQLLL
Sbjct: 181 SPVPKSLPVQWIDATLSKGSEVEFFHDEFRCPVYVKDVVNVIITLIKTWISEGKQMQLLL 240

Query: 241 NVGGPNRVSRVEMAETVAEIRGHKKSLIKRVSASSIDRGVKSPADISMNISKLIQILAMS 300
           NVGGPNRVSRVEMAETVAEIRGHKKSLIKRVSASSIDRGVKSPADISMNISKL+QILA+S
Sbjct: 241 NVGGPNRVSRVEMAETVAEIRGHKKSLIKRVSASSIDRGVKSPADISMNISKLVQILAIS 300

Query: 301 PVPFTDGVRLTIAAEITE 319
           PV FTDGVRLT+A E TE
Sbjct: 301 PVSFTDGVRLTLATETTE 318

BLAST of PI0022432 vs. NCBI nr
Match: XP_023513753.1 (uncharacterized protein LOC111778267 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 554.3 bits (1427), Expect = 6.9e-154
Identity = 277/318 (87.11%), Postives = 295/318 (92.77%), Query Frame = 0

Query: 1   MRKKKVVVVGGTGYLGQHLLSGFSEIDGVPYDIAFTYNSFAPEDLLGALPHLLAFQVDLK 60
           M K KV+V+GGTGYLGQHLLSGFS+IDGVPYD+AFTY+SFAP+DL+ ALPHLLAFQVDLK
Sbjct: 1   MSKNKVLVLGGTGYLGQHLLSGFSDIDGVPYDLAFTYHSFAPDDLVRALPHLLAFQVDLK 60

Query: 61  SGQGFDTIAENFGQPDIVVNCAAISVPRACEIDPVSAFSVNVPTALGTWLLSFEGRNTLF 120
           SGQGFD I + FGQPDIVVNCAAISVPRACE DP SA S+NVPTALG WLLSFE  NTL 
Sbjct: 61  SGQGFDAILQKFGQPDIVVNCAAISVPRACETDPASALSINVPTALGNWLLSFEQMNTLL 120

Query: 121 IHLSTDQVYEGVKSFYNEEDETIPVNVYGKSKLAAEQYVSEKFSNFAILRSSIIFGPQTI 180
           IHLSTDQVYEG+KSFY E+DETIPVNVYGKSKLAAEQYVSEKFSNFAILRSSIIFGPQTI
Sbjct: 121 IHLSTDQVYEGMKSFYKEDDETIPVNVYGKSKLAAEQYVSEKFSNFAILRSSIIFGPQTI 180

Query: 181 SPVPKSLPVQWIDATLSEGSEVEFFHDEFRCPVYVKDVVNVIITLIKTWISEGKQMQLLL 240
           SPV KSLPVQWIDA LS+GSEVEFFHDEFRCPVYVKDVVNVIITLIKTWISEGKQMQL+L
Sbjct: 181 SPVHKSLPVQWIDAALSKGSEVEFFHDEFRCPVYVKDVVNVIITLIKTWISEGKQMQLVL 240

Query: 241 NVGGPNRVSRVEMAETVAEIRGHKKSLIKRVSASSIDRGVKSPADISMNISKLIQILAMS 300
           NVGGPNR+SRVEMAETVAEIRGH KSLIKRVSASSIDRGVKSPADISMNIS L+Q LA+S
Sbjct: 241 NVGGPNRLSRVEMAETVAEIRGHNKSLIKRVSASSIDRGVKSPADISMNISNLVQKLAIS 300

Query: 301 PVPFTDGVRLTIAAEITE 319
           PV F +GVRLT+AAE  E
Sbjct: 301 PVSFNEGVRLTLAAETKE 318

BLAST of PI0022432 vs. NCBI nr
Match: XP_022999832.1 (uncharacterized protein LOC111494187 [Cucurbita maxima])

HSP 1 Score: 552.0 bits (1421), Expect = 3.4e-153
Identity = 274/318 (86.16%), Postives = 296/318 (93.08%), Query Frame = 0

Query: 1   MRKKKVVVVGGTGYLGQHLLSGFSEIDGVPYDIAFTYNSFAPEDLLGALPHLLAFQVDLK 60
           M KKKV+V+GGTGYLGQHLLSGFS+IDGVPYD+AFTY+SFAP+DL+ ALPHLLAFQVDLK
Sbjct: 1   MSKKKVLVLGGTGYLGQHLLSGFSDIDGVPYDLAFTYHSFAPDDLVRALPHLLAFQVDLK 60

Query: 61  SGQGFDTIAENFGQPDIVVNCAAISVPRACEIDPVSAFSVNVPTALGTWLLSFEGRNTLF 120
           SGQGFD I + FGQPDIVVNCAA+SVPRACE DP SA S+NVPTALG WLLSFE  NTL 
Sbjct: 61  SGQGFDAIVQKFGQPDIVVNCAAVSVPRACETDPASALSINVPTALGNWLLSFEQMNTLL 120

Query: 121 IHLSTDQVYEGVKSFYNEEDETIPVNVYGKSKLAAEQYVSEKFSNFAILRSSIIFGPQTI 180
           IHLSTDQVYEG++SFY E+DETIPVNVYGKSKLAAEQYVSEKFSNFAILRSSIIFGPQTI
Sbjct: 121 IHLSTDQVYEGMRSFYKEDDETIPVNVYGKSKLAAEQYVSEKFSNFAILRSSIIFGPQTI 180

Query: 181 SPVPKSLPVQWIDATLSEGSEVEFFHDEFRCPVYVKDVVNVIITLIKTWISEGKQMQLLL 240
           SPV KSLPVQWIDA LS+G+EVEFFHDEFRCPVYVKDVV+VIITLIKTWISEGKQMQL+L
Sbjct: 181 SPVHKSLPVQWIDAALSKGNEVEFFHDEFRCPVYVKDVVSVIITLIKTWISEGKQMQLVL 240

Query: 241 NVGGPNRVSRVEMAETVAEIRGHKKSLIKRVSASSIDRGVKSPADISMNISKLIQILAMS 300
           NVGGPNR+SRVEMAETVAEIRGH KSLIKRVSASSIDRGVKSPADISMNIS L+Q LA+S
Sbjct: 241 NVGGPNRLSRVEMAETVAEIRGHNKSLIKRVSASSIDRGVKSPADISMNISNLVQKLAIS 300

Query: 301 PVPFTDGVRLTIAAEITE 319
           PV F +GVRLT+AAE  E
Sbjct: 301 PVSFNEGVRLTLAAETKE 318

BLAST of PI0022432 vs. TAIR 10
Match: AT4G00560.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 414.1 bits (1063), Expect = 1.0e-115
Identity = 206/318 (64.78%), Postives = 254/318 (79.87%), Query Frame = 0

Query: 2   RKKKVVVVGGTGYLGQHLLSGFSEIDGVP---YDIAFTYNSF-APEDLLGALPHLLAFQV 61
           +K KV++VGGTGYLGQHLL  F+   G     YD+AFT++S   P  LL A PH  AF V
Sbjct: 3   KKTKVLIVGGTGYLGQHLLQAFAGNYGGECELYDVAFTHHSSPLPARLLDAFPHSPAFPV 62

Query: 62  DLKSGQGFDTIAENFGQPDIVVNCAAISVPRACEIDPVSAFSVNVPTALGTWLLSFEGRN 121
           DLKSG G ++I+++F QPD+VVNCAA+SVPRACE DP SA S+NVPT+L  WL SFE   
Sbjct: 63  DLKSGLGLNSISQDFRQPDVVVNCAALSVPRACEQDPDSAMSINVPTSLVNWLSSFETNK 122

Query: 122 TLFIHLSTDQVYEGVKSFYNEEDETIPVNVYGKSKLAAEQYVSEKFSNFAILRSSIIFGP 181
           TL IHLSTDQVY+GVKSFY EEDET+ VNVYGKSK+AAE  + +K  +FAILRSSIIFGP
Sbjct: 123 TLLIHLSTDQVYQGVKSFYKEEDETVAVNVYGKSKVAAELLIKDKCQSFAILRSSIIFGP 182

Query: 182 QTISPVPKSLPVQWIDATLSEGSEVEFFHDEFRCPVYVKDVVNVIITLIKTWISEGKQMQ 241
           QT+SP+PK+LP+QWID++L +G  V+FFHDEFRCP+YVKD+VN+   LI  W+S+ KQM+
Sbjct: 183 QTVSPLPKTLPIQWIDSSLKKGDTVDFFHDEFRCPIYVKDLVNITFKLIDRWVSDDKQMR 242

Query: 242 LLLNVGGPNRVSRVEMAETVAEIRGHKKSLIKRVSASSIDRGVKSPADISMNISKLIQIL 301
           L+LN GGP R+SRV+MA+ VAE+RG+  SLIK VSASSIDRGV SPADISM+I+KLI  L
Sbjct: 243 LVLNAGGPERLSRVQMAQMVAEVRGYDLSLIKHVSASSIDRGVVSPADISMDITKLIHTL 302

Query: 302 AMSPVPFTDGVRLTIAAE 316
            +SP  F +GVRLT+ +E
Sbjct: 303 ELSPTSFKEGVRLTLDSE 320

BLAST of PI0022432 vs. TAIR 10
Match: AT4G00560.4 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 408.3 bits (1048), Expect = 5.7e-114
Identity = 206/322 (63.98%), Postives = 254/322 (78.88%), Query Frame = 0

Query: 2   RKKKVVVVGGTGYLGQHLLSGFSEIDGVP---YDIAFTYNSF-APEDLLGALPHLLAFQV 61
           +K KV++VGGTGYLGQHLL  F+   G     YD+AFT++S   P  LL A PH  AF V
Sbjct: 3   KKTKVLIVGGTGYLGQHLLQAFAGNYGGECELYDVAFTHHSSPLPARLLDAFPHSPAFPV 62

Query: 62  DLKSGQGFDTIAENFGQPDIVVNCAAISVPRACEIDPVSAFSVNVPTALGTWLLSFEGRN 121
           DLKSG G ++I+++F QPD+VVNCAA+SVPRACE DP SA S+NVPT+L  WL SFE   
Sbjct: 63  DLKSGLGLNSISQDFRQPDVVVNCAALSVPRACEQDPDSAMSINVPTSLVNWLSSFETNK 122

Query: 122 TLFIHLSTDQVYEGVKSFYNEEDETIPVNVYGKSKLAAEQYVSEKFSNFAILRSSIIFGP 181
           TL IHLSTDQVY+GVKSFY EEDET+ VNVYGKSK+AAE  + +K  +FAILRSSIIFGP
Sbjct: 123 TLLIHLSTDQVYQGVKSFYKEEDETVAVNVYGKSKVAAELLIKDKCQSFAILRSSIIFGP 182

Query: 182 QTISPVPKSLPVQWIDATLSEGSEVEFFHDEFRCPVYVKDVVNVIITLIKTWISEGKQMQ 241
           QT+SP+PK+LP+QWID++L +G  V+FFHDEFRCP+YVKD+VN+   LI  W+S+ KQM+
Sbjct: 183 QTVSPLPKTLPIQWIDSSLKKGDTVDFFHDEFRCPIYVKDLVNITFKLIDRWVSDDKQMR 242

Query: 242 LLLNVGGPNRVSRVEMAETVAEIRGHKKSLIKRVSASS----IDRGVKSPADISMNISKL 301
           L+LN GGP R+SRV+MA+ VAE+RG+  SLIK VSASS    IDRGV SPADISM+I+KL
Sbjct: 243 LVLNAGGPERLSRVQMAQMVAEVRGYDLSLIKHVSASSLFCQIDRGVVSPADISMDITKL 302

Query: 302 IQILAMSPVPFTDGVRLTIAAE 316
           I  L +SP  F +GVRLT+ +E
Sbjct: 303 IHTLELSPTSFKEGVRLTLDSE 324

BLAST of PI0022432 vs. TAIR 10
Match: AT4G00560.3 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 312.0 bits (798), Expect = 5.5e-85
Identity = 151/234 (64.53%), Postives = 185/234 (79.06%), Query Frame = 0

Query: 2   RKKKVVVVGGTGYLGQHLLSGFSEIDGVP---YDIAFTYNSF-APEDLLGALPHLLAFQV 61
           +K KV++VGGTGYLGQHLL  F+   G     YD+AFT++S   P  LL A PH  AF V
Sbjct: 3   KKTKVLIVGGTGYLGQHLLQAFAGNYGGECELYDVAFTHHSSPLPARLLDAFPHSPAFPV 62

Query: 62  DLKSGQGFDTIAENFGQPDIVVNCAAISVPRACEIDPVSAFSVNVPTALGTWLLSFEGRN 121
           DLKSG G ++I+++F QPD+VVNCAA+SVPRACE DP SA S+NVPT+L  WL SFE   
Sbjct: 63  DLKSGLGLNSISQDFRQPDVVVNCAALSVPRACEQDPDSAMSINVPTSLVNWLSSFETNK 122

Query: 122 TLFIHLSTDQVYEGVKSFYNEEDETIPVNVYGKSKLAAEQYVSEKFSNFAILRSSIIFGP 181
           TL IHLSTDQVY+GVKSFY EEDET+ VNVYGKSK+AAE  + +K  +FAILRSSIIFGP
Sbjct: 123 TLLIHLSTDQVYQGVKSFYKEEDETVAVNVYGKSKVAAELLIKDKCQSFAILRSSIIFGP 182

Query: 182 QTISPVPKSLPVQWIDATLSEGSEVEFFHDEFRCPVYVKDVVNVIITLIKTWIS 232
           QT+SP+PK+LP+QWID++L +G  V+FFHDEFRCP+YVKD+VN+   LI  W+S
Sbjct: 183 QTVSPLPKTLPIQWIDSSLKKGDTVDFFHDEFRCPIYVKDLVNITFKLIDRWVS 236

BLAST of PI0022432 vs. TAIR 10
Match: AT4G00560.2 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 252.3 bits (643), Expect = 5.2e-67
Identity = 127/196 (64.80%), Postives = 154/196 (78.57%), Query Frame = 0

Query: 2   RKKKVVVVGGTGYLGQHLLSGFSEIDGVP---YDIAFTYNSF-APEDLLGALPHLLAFQV 61
           +K KV++VGGTGYLGQHLL  F+   G     YD+AFT++S   P  LL A PH  AF V
Sbjct: 3   KKTKVLIVGGTGYLGQHLLQAFAGNYGGECELYDVAFTHHSSPLPARLLDAFPHSPAFPV 62

Query: 62  DLKSGQGFDTIAENFGQPDIVVNCAAISVPRACEIDPVSAFSVNVPTALGTWLLSFEGRN 121
           DLKSG G ++I+++F QPD+VVNCAA+SVPRACE DP SA S+NVPT+L  WL SFE   
Sbjct: 63  DLKSGLGLNSISQDFRQPDVVVNCAALSVPRACEQDPDSAMSINVPTSLVNWLSSFETNK 122

Query: 122 TLFIHLSTDQVYEGVKSFYNEEDETIPVNVYGKSKLAAEQYVSEKFSNFAILRSSIIFGP 181
           TL IHLSTDQVY+GVKSFY EEDET+ VNVYGKSK+AAE  + +K  +FAILRSSIIFGP
Sbjct: 123 TLLIHLSTDQVYQGVKSFYKEEDETVAVNVYGKSKVAAELLIKDKCQSFAILRSSIIFGP 182

Query: 182 QTISPVPKSLPVQWID 194
           QT+SP+PK+LP+Q I+
Sbjct: 183 QTVSPLPKTLPIQMIN 198

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
D4GU713.0e-1928.46Probable low-salt glycan biosynthesis reductase Agl14 OS=Haloferax volcanii (str... [more]
Q99LB62.8e-1724.51Methionine adenosyltransferase 2 subunit beta OS=Mus musculus OX=10090 GN=Mat2b ... [more]
Q9NZL96.3e-1725.49Methionine adenosyltransferase 2 subunit beta OS=Homo sapiens OX=9606 GN=MAT2B P... [more]
Q5R4E06.3e-1725.49Methionine adenosyltransferase 2 subunit beta OS=Pongo abelii OX=9601 GN=MAT2B P... [more]
Q5U2R06.3e-1724.51Methionine adenosyltransferase 2 subunit beta OS=Rattus norvegicus OX=10116 GN=M... [more]
Match NameE-valueIdentityDescription
A0A5D3DLR18.7e-17196.54Putative dTDP-4-dehydrorhamnose reductase OS=Cucumis melo var. makuwa OX=1194695... [more]
A0A1S3CD698.7e-17196.54probable dTDP-4-dehydrorhamnose reductase OS=Cucumis melo OX=3656 GN=LOC10349906... [more]
A0A0A0KEJ71.2e-16795.54RmlD_sub_bind domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G0740... [more]
A0A6J1KBW11.6e-15386.16uncharacterized protein LOC111494187 OS=Cucurbita maxima OX=3661 GN=LOC111494187... [more]
A0A6J1DR992.4e-15286.98methionine adenosyltransferase 2 subunit beta OS=Momordica charantia OX=3673 GN=... [more]
Match NameE-valueIdentityDescription
XP_008460177.11.8e-17096.54PREDICTED: probable dTDP-4-dehydrorhamnose reductase [Cucumis melo] >TYK24462.1 ... [more]
XP_004144979.12.4e-16795.54uncharacterized protein LOC101221549 [Cucumis sativus] >KGN46196.1 hypothetical ... [more]
XP_038878319.16.9e-16292.14methionine adenosyltransferase 2 subunit beta [Benincasa hispida][more]
XP_023513753.16.9e-15487.11uncharacterized protein LOC111778267 [Cucurbita pepo subsp. pepo][more]
XP_022999832.13.4e-15386.16uncharacterized protein LOC111494187 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT4G00560.11.0e-11564.78NAD(P)-binding Rossmann-fold superfamily protein [more]
AT4G00560.45.7e-11463.98NAD(P)-binding Rossmann-fold superfamily protein [more]
AT4G00560.35.5e-8564.53NAD(P)-binding Rossmann-fold superfamily protein [more]
AT4G00560.25.2e-6764.80NAD(P)-binding Rossmann-fold superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.40.50.720coord: 2..316
e-value: 1.1E-59
score: 204.5
NoneNo IPR availablePANTHERPTHR43242NAD(P)-BINDING ROSSMANN-FOLD SUPERFAMILY PROTEINcoord: 3..315
NoneNo IPR availableCDDcd05254dTDP_HR_like_SDR_ecoord: 5..309
e-value: 9.86647E-75
score: 229.436
IPR029903RmlD-like substrate binding domainPFAMPF04321RmlD_sub_bindcoord: 5..313
e-value: 8.5E-39
score: 133.3
IPR036291NAD(P)-binding domain superfamilySUPERFAMILY51735NAD(P)-binding Rossmann-fold domainscoord: 5..313

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0022432.1PI0022432.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016740 transferase activity