PI0018885 (gene) Melon (PI 482460) v1

Overview
NamePI0018885
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
Descriptionextradiol ring-cleavage dioxygenase
Locationchr08: 9882721 .. 9883899 (+)
RNA-Seq ExpressionPI0018885
SyntenyPI0018885
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTTTGAAAGACACTTTCTATGTATCTCATGGCTCTCCCACCCTAACCATCGATGATACCATACAAGCAAGACAGTTCTTCCAAACATGGAAGGAGAAGGTTTTCCCACAAAAACCTAAAGCCATTCTTTGCATTTCTGCCCATTACGACACCACGTACCCTACTGTGAATGTGGTTTCGGGCCCCAGTGACACCATCTACGACTTCTATGGCTTCCCATCGGAAATGTACAAGGTAAATTTAAGTCTCTGTTTGGTAGAAAATTTTGTTTAGGTTTTTAGATTCATTGTATTAAAAAAAGTATAATATGTATTTGACATACCATCTAAATTATAGTTCTGAAAATTGTTTTTCGATTAATCTTATGAATAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATGAAAAAAAAATTGAAACTATTTACAAAATATAGAAAAAATAGTTAAAAAAAAAATCATTATAACGAGGATGCATGCAGCTAAAATATCCGGCTCGGGAGCTGCAGTGCTGGCAAAGAAGGTGAAGGAAGCGCTAATAGGGGCGGGATTCGAGGGTGTGGAGGAGGACAAGGGGCGAGGGTTGGATCATGGAGCATGGGTGCCATTGATGTTCATGTATCCAGAGGCTGATATCCCAGTTTGCCAACTTTCAGTACAAACAAGCAAAATGGGACACATCATTACAACATGGGAAAGGCTTTGGCTTTTCTTAAAGATGAAGGAGTTTTGATTTTTGGGTCAGGAAGTGCTACACACAATCTTAGAACACTTGACTTTGATGCAAGATCTCGTGCTACTGCCTCATGGGCTATTGACTTTGATAAGTGGCTTCAACAAACTCTCCTTCAAGGAAGGTATATATACGCCTACATATATATATACATCATTATTAAATAATATATAATTAAGCTATATTAATTAAAAATGTGTTGATTGTTTTATTATTATTATTAATTTTTTTTTGTGTAATAATAATAGATATGAAGATGTGAATGAATATGAGAAGAAGGCTCCAAATCCAAAAATGGCACATCCAAGTCCAGATCATTTTTACCCACTACATGTTGCAATTGGGGCTGCTGGAGGCCAACCAAAAGCAAAGCTAATCCACCATAGTTGGGACCTTGGCACCATGTCCTATGCCTCCTACCAATTCACTTCCCCTTAA

mRNA sequence

ATGGCTTTGAAAGACACTTTCTATGTATCTCATGGCTCTCCCACCCTAACCATCGATGATACCATACAAGCAAGACAGTTCTTCCAAACATGGAAGGAGAAGGTTTTCCCACAAAAACCTAAAGCCATTCTTTGCATTTCTGCCCATTACGACACCACGTACCCTACTGTGAATGTGGTTTCGGGCCCCAGTGACACCATCTACGACTTCTATGGCTTCCCATCGGAAATGTACAAGGTAAATTTAACTAAAATATCCGGCTCGGGAGCTGCAGTGCTGGCAAAGAAGGTGAAGGAAGCGCTAATAGGGGCGGGATTCGAGGGTGTGGAGGAGGACAAGGGGCGAGGGTTGGATCATGGAGCATGGGTGCCATTGATGTTCATGTATCCAGAGGCTGATATCCCAGTTTGCCAACTTTCAGCTTTGGCTTTTCTTAAAGATGAAGGAGTTTTGATTTTTGGGTCAGGAAGTGCTACACACAATCTTAGAACACTTGACTTTGATGCAAGATCTCGTGCTACTGCCTCATGGGCTATTGACTTTGATAAGTGGCTTCAACAAACTCTCCTTCAAGGAAGATATGAAGATGTGAATGAATATGAGAAGAAGGCTCCAAATCCAAAAATGGCACATCCAAGTCCAGATCATTTTTACCCACTACATGTTGCAATTGGGGCTGCTGGAGGCCAACCAAAAGCAAAGCTAATCCACCATAGTTGGGACCTTGGCACCATGTCCTATGCCTCCTACCAATTCACTTCCCCTTAA

Coding sequence (CDS)

ATGGCTTTGAAAGACACTTTCTATGTATCTCATGGCTCTCCCACCCTAACCATCGATGATACCATACAAGCAAGACAGTTCTTCCAAACATGGAAGGAGAAGGTTTTCCCACAAAAACCTAAAGCCATTCTTTGCATTTCTGCCCATTACGACACCACGTACCCTACTGTGAATGTGGTTTCGGGCCCCAGTGACACCATCTACGACTTCTATGGCTTCCCATCGGAAATGTACAAGGTAAATTTAACTAAAATATCCGGCTCGGGAGCTGCAGTGCTGGCAAAGAAGGTGAAGGAAGCGCTAATAGGGGCGGGATTCGAGGGTGTGGAGGAGGACAAGGGGCGAGGGTTGGATCATGGAGCATGGGTGCCATTGATGTTCATGTATCCAGAGGCTGATATCCCAGTTTGCCAACTTTCAGCTTTGGCTTTTCTTAAAGATGAAGGAGTTTTGATTTTTGGGTCAGGAAGTGCTACACACAATCTTAGAACACTTGACTTTGATGCAAGATCTCGTGCTACTGCCTCATGGGCTATTGACTTTGATAAGTGGCTTCAACAAACTCTCCTTCAAGGAAGATATGAAGATGTGAATGAATATGAGAAGAAGGCTCCAAATCCAAAAATGGCACATCCAAGTCCAGATCATTTTTACCCACTACATGTTGCAATTGGGGCTGCTGGAGGCCAACCAAAAGCAAAGCTAATCCACCATAGTTGGGACCTTGGCACCATGTCCTATGCCTCCTACCAATTCACTTCCCCTTAA

Protein sequence

MALKDTFYVSHGSPTLTIDDTIQARQFFQTWKEKVFPQKPKAILCISAHYDTTYPTVNVVSGPSDTIYDFYGFPSEMYKVNLTKISGSGAAVLAKKVKEALIGAGFEGVEEDKGRGLDHGAWVPLMFMYPEADIPVCQLSALAFLKDEGVLIFGSGSATHNLRTLDFDARSRATASWAIDFDKWLQQTLLQGRYEDVNEYEKKAPNPKMAHPSPDHFYPLHVAIGAAGGQPKAKLIHHSWDLGTMSYASYQFTSP
Homology
BLAST of PI0018885 vs. ExPASy Swiss-Prot
Match: Q949R4 (Extradiol ring-cleavage dioxygenase OS=Arabidopsis thaliana OX=3702 GN=LIGB PE=2 SV=1)

HSP 1 Score: 325.5 bits (833), Expect = 5.5e-88
Identity = 162/268 (60.45%), Postives = 198/268 (73.88%), Query Frame = 0

Query: 3   LKDTFYVSHGSPTLTIDDTIQARQFFQTWKEKVFPQKPKAILCISAHYDTTYPTVNVVSG 62
           +  TF++SHGSPTL+IDD+++ARQFF++W +KV PQKPK+IL ISAH+DT +P+VN V  
Sbjct: 4   VNQTFFLSHGSPTLSIDDSLEARQFFKSWTQKVLPQKPKSILVISAHWDTKFPSVNTVL- 63

Query: 63  PSDTIYDFYGFPSEMYKVNLTKISGSGAAVLAKKVKEALI-GAGFEGVEEDKGRGLDHGA 122
            ++TI+DF GFP  MYK+   K    GA  L K+VKE L+   G + V+ED  RGLDHGA
Sbjct: 64  RNNTIHDFSGFPDPMYKL---KYEAPGAIELGKRVKELLMKEGGMKRVDEDTKRGLDHGA 123

Query: 123 WVPLMFMYPEADIPVCQLS---------------ALAFLKDEGVLIFGSGSATHNLRTLD 182
           WVPLM MYPEADIP+CQLS               ALA LKDEGVLI GSGSATHNLR LD
Sbjct: 124 WVPLMLMYPEADIPICQLSVQSNQNGSYHYNMGKALASLKDEGVLIIGSGSATHNLRKLD 183

Query: 183 FDARSRATASWAIDFDKWLQQTLLQGRYEDVNEYEKKAPNPKMAHPSPDHFYPLHVAIGA 242
           F+    +   WA++FD WL+ +LLQGRY DVNE+E+KAPN KMAHP P+H YPLHV +GA
Sbjct: 184 FNITDGSPVPWALEFDHWLRDSLLQGRYGDVNEWEEKAPNAKMAHPWPEHLYPLHVVMGA 243

Query: 243 AGGQPKAKLIHHSWDLGTMSYASYQFTS 255
           AGG  KA+ IH SW LGT+SY+SY FTS
Sbjct: 244 AGGDAKAEQIHTSWQLGTLSYSSYSFTS 267

BLAST of PI0018885 vs. ExPASy Swiss-Prot
Match: Q70FG7 (4,5-DOPA dioxygenase extradiol OS=Beta vulgaris OX=161934 GN=DODA PE=1 SV=1)

HSP 1 Score: 297.0 bits (759), Expect = 2.1e-79
Identity = 150/267 (56.18%), Postives = 189/267 (70.79%), Query Frame = 0

Query: 3   LKDTFYVSHGSPTLTIDDTIQARQFFQTWKEKVFPQKPKAILCISAHYDTTYPTVNVVSG 62
           +K+TF++SHG+P + IDD+  +++F ++W+EK+F +KPKAIL ISAH++T  P+VNVV  
Sbjct: 7   IKETFFISHGTPMMAIDDSKPSKKFLESWREKIFSKKPKAILVISAHWETDQPSVNVVD- 66

Query: 63  PSDTIYDFYGFPSEMYKVNLTKISGSGAAVLAKKVKEALIGAGFEGVEEDKGRGLDHGAW 122
            +DTIYDF GFP+ +Y+    K S  G+  LA ++++ L G+GF+ V  DK RGLDHGAW
Sbjct: 67  INDTIYDFRGFPARLYQF---KYSAPGSPELANRIQDLLAGSGFKSVNTDKKRGLDHGAW 126

Query: 123 VPLMFMYPEADIPVCQLS---------------ALAFLKDEGVLIFGSGSATHNLRTLDF 182
           VPLM MYPEADIPVCQLS               ALA LKDEGVLI GSGSATH   +   
Sbjct: 127 VPLMLMYPEADIPVCQLSVQSHLDGTHHYKLGQALAPLKDEGVLIIGSGSATH--PSNGT 186

Query: 183 DARSRATASWAIDFDKWLQQTLLQGRYEDVNEYEKKAPNPKMAHPSPDHFYPLHVAIGAA 242
              S   A WA  FD WL+  L  G YE+VN+YE KAPN K+AHP P+HFYPLHVA+GAA
Sbjct: 187 PPCSDGVAPWAAAFDSWLETALTNGSYEEVNKYETKAPNWKLAHPWPEHFYPLHVAMGAA 246

Query: 243 GGQPKAKLIHHSWDLGTMSYASYQFTS 255
           G   KA+LIH+SWD G MSY SY+FTS
Sbjct: 247 GENSKAELIHNSWDGGIMSYGSYKFTS 267

BLAST of PI0018885 vs. ExPASy Swiss-Prot
Match: I3PFJ3 (4,5-DOPA dioxygenase extradiol 1 OS=Beta vulgaris OX=161934 GN=DODA1 PE=2 SV=1)

HSP 1 Score: 287.0 bits (733), Expect = 2.1e-76
Identity = 149/269 (55.39%), Postives = 187/269 (69.52%), Query Frame = 0

Query: 3   LKDTFYVSHGSPTLTIDDTIQARQFFQTWKEKVFPQKPKAILCISAHYDTTYPTVNVVSG 62
           +K++F+++HG+P LT++DT   R FF+TW+EK+F +KPKAIL IS H++T  PTVN V  
Sbjct: 14  IKESFFITHGNPILTVEDTHPLRPFFETWREKIFSKKPKAILIISGHWETVKPTVNAVH- 73

Query: 63  PSDTIYDFYGFPSEMYKVNLTKISGSGAAVLAKKVKEALIGAGFEGVEEDKGRGLDHGAW 122
            +DTI+DF  +P+ MY   L K    GA  LA+KV+E L  +GFE  E D+ RGLDHGAW
Sbjct: 74  INDTIHDFDDYPAAMY---LFKYPAPGAPELARKVEEILKKSGFETAETDEKRGLDHGAW 133

Query: 123 VPLMFMYPEADIPVCQLS---------------ALAFLKDEGVLIFGSGSATHNLRTLD- 182
           VPLM MYPEADIPVCQLS               ALA LK++GVLI GSGSATH L     
Sbjct: 134 VPLMLMYPEADIPVCQLSVQPHLDGTYHYNLGRALAPLKNDGVLIIGSGSATHPLDETPH 193

Query: 183 -FDARSRATASWAIDFDKWLQQTLLQGRYEDVNEYEKKAPNPKMAHPSPDHFYPLHVAIG 242
            FD      A WA  FD WL++ L+ GR+E+VN YE KAPN K+AHP P+HFYPLHV +G
Sbjct: 194 YFD----GVAPWAAAFDSWLRKALINGRFEEVNIYETKAPNWKLAHPFPEHFYPLHVVLG 253

Query: 243 AAGGQPKAKLIHHSWDLGTMSYASYQFTS 255
           AAG + KA+LIH SWD GT+ + SY+FTS
Sbjct: 254 AAGEKWKAELIHSSWDHGTLCHGSYKFTS 274

BLAST of PI0018885 vs. ExPASy Swiss-Prot
Match: I3PFJ9 (4,5-DOPA dioxygenase extradiol 1 OS=Beta vulgaris OX=161934 GN=DODA1 PE=2 SV=1)

HSP 1 Score: 284.3 bits (726), Expect = 1.4e-75
Identity = 147/269 (54.65%), Postives = 186/269 (69.14%), Query Frame = 0

Query: 3   LKDTFYVSHGSPTLTIDDTIQARQFFQTWKEKVFPQKPKAILCISAHYDTTYPTVNVVSG 62
           +K++F+++HG+P LT++DT   R FF+TW+EK+F +KPKAIL IS H++T  PTVN V  
Sbjct: 14  IKESFFITHGNPILTVEDTHPLRPFFETWREKIFSKKPKAILIISGHWETVKPTVNAVH- 73

Query: 63  PSDTIYDFYGFPSEMYKVNLTKISGSGAAVLAKKVKEALIGAGFEGVEEDKGRGLDHGAW 122
            +DTI+DF  +P+ MY+    K    G   LA+KV+E L  +GFE  E D+ RGLDHGAW
Sbjct: 74  INDTIHDFDDYPAAMYQF---KYPAPGEPELARKVEEILKKSGFETAETDQKRGLDHGAW 133

Query: 123 VPLMFMYPEADIPVCQLS---------------ALAFLKDEGVLIFGSGSATHNLRTLD- 182
           VPLM MYPEADIPVCQLS               ALA LK++GVLI GSGSATH L     
Sbjct: 134 VPLMLMYPEADIPVCQLSVQPHLDGTYHYNLGRALAPLKNDGVLIIGSGSATHPLDETPH 193

Query: 183 -FDARSRATASWAIDFDKWLQQTLLQGRYEDVNEYEKKAPNPKMAHPSPDHFYPLHVAIG 242
            FD      A WA  FD WL++ L+ GR+E+VN YE KAPN K+AHP P+HFYPLHV +G
Sbjct: 194 YFD----GVAPWAAAFDSWLRKALINGRFEEVNIYESKAPNWKLAHPFPEHFYPLHVVLG 253

Query: 243 AAGGQPKAKLIHHSWDLGTMSYASYQFTS 255
           AAG + KA+LIH SWD GT+ + SY+FTS
Sbjct: 254 AAGEKWKAELIHSSWDHGTLCHGSYKFTS 274

BLAST of PI0018885 vs. ExPASy Swiss-Prot
Match: Q7XA48 (4,5-DOPA dioxygenase extradiol OS=Portulaca grandiflora OX=3583 GN=DODA PE=1 SV=1)

HSP 1 Score: 248.8 bits (634), Expect = 6.5e-65
Identity = 130/270 (48.15%), Postives = 172/270 (63.70%), Query Frame = 0

Query: 1   MALKDTFYVSHGSPTLTIDDTIQARQFFQTWKEKVFPQKPKAILCISAHYDTTYPTVNVV 60
           ++ K++F++SHG+P +  D++  AR F   WK+ VFP KPK+IL +SAH++T  P V+  
Sbjct: 7   VSFKESFFLSHGNPAMLADESFIARNFLLGWKKNVFPVKPKSILVVSAHWETDVPCVSAG 66

Query: 61  SGPSDTIYDFYGFPSEMYKVNLTKISGSGAAVLAKKVKEALIGAGFEGVEEDKGRGLDHG 120
             P + IYDF   P+ M+++   K    G   LAK+V+E LI  GF+  + D+ RG DH 
Sbjct: 67  QYP-NVIYDFTEVPASMFQM---KYPAPGCPKLAKRVQELLIAGGFKSAKLDEERGFDHS 126

Query: 121 AWVPLMFMYPEADIPVCQLS---------------ALAFLKDEGVLIFGSGSATHNLRTL 180
           +WVPL  M PEADIPVCQLS               ALA LK EGVL  GSG A H   + 
Sbjct: 127 SWVPLSMMCPEADIPVCQLSVQPGLDATHHFNVGRALAPLKGEGVLFIGSGGAVH--PSD 186

Query: 181 DFDARSRATASWAIDFDKWLQQTLLQGRYEDVNEYEKKAPNP-KMAHPSPDHFYPLHVAI 240
           D        A WA +FD+WL+  LL+GRYEDVN Y+ KAP   K+AHP P+HF PLHVA+
Sbjct: 187 DTPHWFDGVAPWAAEFDQWLEDALLEGRYEDVNNYQTKAPEGWKLAHPIPEHFLPLHVAM 246

Query: 241 GAAGGQPKAKLIHHSWDLGTMSYASYQFTS 255
           GA G + KA+LI+ +WD GT+ YASY+FTS
Sbjct: 247 GAGGEKSKAELIYRTWDHGTLGYASYKFTS 270

BLAST of PI0018885 vs. ExPASy TrEMBL
Match: A0A6J1D7R1 (extradiol ring-cleavage dioxygenase OS=Momordica charantia OX=3673 GN=LOC111017797 PE=3 SV=1)

HSP 1 Score: 419.5 bits (1077), Expect = 1.0e-113
Identity = 204/270 (75.56%), Postives = 227/270 (84.07%), Query Frame = 0

Query: 1   MALKDTFYVSHGSPTLTIDDTIQARQFFQTWKEKVFPQKPKAILCISAHYDTTYPTVNVV 60
           MA+ DTFY+SHGSPTL+IDDTI+ARQFFQ+WK+KVFPQKPK+ILCISAHYDT++PT+NVV
Sbjct: 1   MAVSDTFYISHGSPTLSIDDTIEARQFFQSWKQKVFPQKPKSILCISAHYDTSFPTLNVV 60

Query: 61  SGPSDTIYDFYGFPSEMYKVNLTKISGSGAAVLAKKVKEALIGAGFEGVEEDKGRGLDHG 120
           SGP+DTIYDFYGFPS MYK+   K    GA  LAK+VKEAL+GAGFE VEE++GRGLDHG
Sbjct: 61  SGPNDTIYDFYGFPSSMYKL---KYPAPGAPALAKRVKEALVGAGFERVEEERGRGLDHG 120

Query: 121 AWVPLMFMYPEADIPVCQLS---------------ALAFLKDEGVLIFGSGSATHNLRTL 180
           AWVPLMFMYPEADIPVCQLS               ALA LKDEGVLI  SGSATHNLRTL
Sbjct: 121 AWVPLMFMYPEADIPVCQLSVQSRKNGTYHYNLGRALAPLKDEGVLIVASGSATHNLRTL 180

Query: 181 DFDARSRATASWAIDFDKWLQQTLLQGRYEDVNEYEKKAPNPKMAHPSPDHFYPLHVAIG 240
           D  A+S   A WA++FD WL+  LLQGRYEDVNEYEKKAP+ KMAHPSPDH YPLHVA+G
Sbjct: 181 DRGAKS--IAPWALEFDNWLKDALLQGRYEDVNEYEKKAPHAKMAHPSPDHLYPLHVAMG 240

Query: 241 AAGGQPKAKLIHHSWDLGTMSYASYQFTSP 256
           AAGG  KAKLIHHSWDLGTMSYASYQFT+P
Sbjct: 241 AAGGNAKAKLIHHSWDLGTMSYASYQFTAP 265

BLAST of PI0018885 vs. ExPASy TrEMBL
Match: A0A6J1F3T4 (extradiol ring-cleavage dioxygenase-like OS=Cucurbita moschata OX=3662 GN=LOC111439883 PE=3 SV=1)

HSP 1 Score: 415.2 bits (1066), Expect = 1.9e-112
Identity = 202/269 (75.09%), Postives = 222/269 (82.53%), Query Frame = 0

Query: 1   MALKDTFYVSHGSPTLTIDDTIQARQFFQTWKEKVFPQKPKAILCISAHYDTTYPTVNVV 60
           MA  DTFYVSHGSPTLTIDD I+AR FF++WK+KVF Q PKAILC+SAH+DT YPTVNVV
Sbjct: 1   MAFNDTFYVSHGSPTLTIDDGIKARHFFKSWKDKVFSQTPKAILCVSAHFDTAYPTVNVV 60

Query: 61  SGPSDTIYDFYGFPSEMYKVNLTKISGSGAAVLAKKVKEALIGAGFEGVEEDKGRGLDHG 120
           SGP+DTIYDFYGFPS MY++   K    GA  LAK+VKEAL+GAGFE V+E+KGRGLDHG
Sbjct: 61  SGPNDTIYDFYGFPSSMYEL---KYPAPGAPALAKRVKEALMGAGFERVDEEKGRGLDHG 120

Query: 121 AWVPLMFMYPEADIPVCQLS---------------ALAFLKDEGVLIFGSGSATHNLRTL 180
           AWVPLMFMYPEADIPVCQLS               ALA LKDEGVLI GSGSATHNLRTL
Sbjct: 121 AWVPLMFMYPEADIPVCQLSVQSRLNGTHHYNVGKALAPLKDEGVLIVGSGSATHNLRTL 180

Query: 181 DFDARSRATASWAIDFDKWLQQTLLQGRYEDVNEYEKKAPNPKMAHPSPDHFYPLHVAIG 240
           D  ARS   ASWAI+FD WL+  LLQGRYEDVNEY+KKAPN + AHPSPDH +PLHVAIG
Sbjct: 181 DRHARSSVNASWAIEFDDWLKHALLQGRYEDVNEYKKKAPNAEKAHPSPDHLFPLHVAIG 240

Query: 241 AAGGQPKAKLIHHSWDLGTMSYASYQFTS 255
           AAGG PKAKL+HHSWDLGTMSYASYQFT+
Sbjct: 241 AAGGHPKAKLVHHSWDLGTMSYASYQFTA 266

BLAST of PI0018885 vs. ExPASy TrEMBL
Match: A0A6J1HS49 (extradiol ring-cleavage dioxygenase-like OS=Cucurbita maxima OX=3661 GN=LOC111467292 PE=3 SV=1)

HSP 1 Score: 411.4 bits (1056), Expect = 2.8e-111
Identity = 200/269 (74.35%), Postives = 221/269 (82.16%), Query Frame = 0

Query: 1   MALKDTFYVSHGSPTLTIDDTIQARQFFQTWKEKVFPQKPKAILCISAHYDTTYPTVNVV 60
           MAL DTFYVSHGSPTLTIDD I+AR FF++WK+KVF Q PKAILC+SAH+DT YPTVNVV
Sbjct: 1   MALNDTFYVSHGSPTLTIDDGIKARHFFKSWKDKVFSQTPKAILCVSAHFDTAYPTVNVV 60

Query: 61  SGPSDTIYDFYGFPSEMYKVNLTKISGSGAAVLAKKVKEALIGAGFEGVEEDKGRGLDHG 120
           SGP+DTIYDFYGFPS MY++   K    GA  LAK+VKEAL+GAGFE V+E+KGRGLDHG
Sbjct: 61  SGPNDTIYDFYGFPSSMYEL---KYPAPGAPALAKRVKEALMGAGFERVDEEKGRGLDHG 120

Query: 121 AWVPLMFMYPEADIPVCQLS---------------ALAFLKDEGVLIFGSGSATHNLRTL 180
           AWVPLMFMYPEADIPVCQLS               ALA LKDEGVL+ GSGSATHNLRTL
Sbjct: 121 AWVPLMFMYPEADIPVCQLSVQSRLNGTHHYNMGKALAPLKDEGVLVVGSGSATHNLRTL 180

Query: 181 DFDARSRATASWAIDFDKWLQQTLLQGRYEDVNEYEKKAPNPKMAHPSPDHFYPLHVAIG 240
           D  ARS   ASWAI+FD WL+  LLQ RYEDVNEY+ KAPN + AHPSPDH +PLHVAIG
Sbjct: 181 DRHARSSVNASWAIEFDDWLKHALLQRRYEDVNEYKNKAPNAEKAHPSPDHLFPLHVAIG 240

Query: 241 AAGGQPKAKLIHHSWDLGTMSYASYQFTS 255
           AAGG PKAKL+HHSWDLGTMSYASYQFT+
Sbjct: 241 AAGGHPKAKLVHHSWDLGTMSYASYQFTA 266

BLAST of PI0018885 vs. ExPASy TrEMBL
Match: A0A6J1HWD7 (extradiol ring-cleavage dioxygenase-like OS=Cucurbita maxima OX=3661 GN=LOC111467255 PE=3 SV=1)

HSP 1 Score: 408.7 bits (1049), Expect = 1.8e-110
Identity = 199/269 (73.98%), Postives = 222/269 (82.53%), Query Frame = 0

Query: 1   MALKDTFYVSHGSPTLTIDDTIQARQFFQTWKEKVFPQKPKAILCISAHYDTTYPTVNVV 60
           MAL DTFY+SHGSPTL+IDD+I+ARQFF++WK +V   KPKAILCISAHYDTT+PTVNVV
Sbjct: 1   MALNDTFYLSHGSPTLSIDDSIKARQFFKSWKNEVVAVKPKAILCISAHYDTTFPTVNVV 60

Query: 61  SGPSDTIYDFYGFPSEMYKVNLTKISGSGAAVLAKKVKEALIGAGFEGVEEDKGRGLDHG 120
           SGP+DTIYDFYGFPS MYK+   K    GA  LAK+VKEAL+ AGFE V+E++GRGLDHG
Sbjct: 61  SGPNDTIYDFYGFPSSMYKI---KYPAPGAPELAKRVKEALMRAGFERVDEERGRGLDHG 120

Query: 121 AWVPLMFMYPEADIPVCQLS---------------ALAFLKDEGVLIFGSGSATHNLRTL 180
           AWVPLMFMYPEADIPVCQLS               ALA LKDEGVLI GSGSATHNLRTL
Sbjct: 121 AWVPLMFMYPEADIPVCQLSVQSHLNGTHHYNLGKALAPLKDEGVLIIGSGSATHNLRTL 180

Query: 181 DFDARSRATASWAIDFDKWLQQTLLQGRYEDVNEYEKKAPNPKMAHPSPDHFYPLHVAIG 240
           +   +  ATA WA++FD WL+  LLQGRY+DVNEYEKKAP+ KMAHPSPDHFYPLHVAIG
Sbjct: 181 NRSGKPSATAPWALEFDTWLKDALLQGRYDDVNEYEKKAPHAKMAHPSPDHFYPLHVAIG 240

Query: 241 AAGGQPKAKLIHHSWDLGTMSYASYQFTS 255
           AAGG PK KLIHHSWD GTMSYASYQFT+
Sbjct: 241 AAGGDPKTKLIHHSWDHGTMSYASYQFTA 266

BLAST of PI0018885 vs. ExPASy TrEMBL
Match: A0A5A7TUJ3 (Extradiol ring-cleavage dioxygenase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold30G001920 PE=3 SV=1)

HSP 1 Score: 407.9 bits (1047), Expect = 3.1e-110
Identity = 197/268 (73.51%), Postives = 221/268 (82.46%), Query Frame = 0

Query: 1   MALKDTFYVSHGSPTLTIDDTIQARQFFQTWKEKVFPQKPKAILCISAHYDTTYPTVNVV 60
           M LK+TFY+SHGSP ++IDD+IQARQFF++WK+ VF  KPKAILC+SAHYDTT+PTVNVV
Sbjct: 1   MGLKETFYLSHGSPMMSIDDSIQARQFFKSWKDNVFVTKPKAILCVSAHYDTTFPTVNVV 60

Query: 61  SGPSDTIYDFYGFPSEMYKVNLTKISGSGAAVLAKKVKEALIGAGFEGVEEDKGRGLDHG 120
           SGP+ TIYDFYGFPS MYK+   K    GA  LAK VKEAL+ AGFE VEE++GRGLDHG
Sbjct: 61  SGPNGTIYDFYGFPSSMYKL---KYPAPGAPALAKSVKEALVRAGFERVEEERGRGLDHG 120

Query: 121 AWVPLMFMYPEADIPVCQLS---------------ALAFLKDEGVLIFGSGSATHNLRTL 180
           AWVPLMFMYPEADIPVCQLS               ALA LKDEGVLI GSGSATHNLRTL
Sbjct: 121 AWVPLMFMYPEADIPVCQLSVQSHLNGTHHYNLGKALAPLKDEGVLIIGSGSATHNLRTL 180

Query: 181 DFDARSRATASWAIDFDKWLQQTLLQGRYEDVNEYEKKAPNPKMAHPSPDHFYPLHVAIG 240
           + +  S A A WA++FD WL+  LLQGRY+DVNEYEKKAP+ +MAHPSPDHF+PLHVAIG
Sbjct: 181 NRNGNSSAIAPWALEFDNWLKDALLQGRYDDVNEYEKKAPHARMAHPSPDHFFPLHVAIG 240

Query: 241 AAGGQPKAKLIHHSWDLGTMSYASYQFT 254
           AAGG PKAKLIHHSWDLGTMSYASYQFT
Sbjct: 241 AAGGNPKAKLIHHSWDLGTMSYASYQFT 265

BLAST of PI0018885 vs. NCBI nr
Match: XP_038883407.1 (extradiol ring-cleavage dioxygenase-like [Benincasa hispida])

HSP 1 Score: 452.6 bits (1163), Expect = 2.3e-123
Identity = 220/269 (81.78%), Postives = 235/269 (87.36%), Query Frame = 0

Query: 1   MALKDTFYVSHGSPTLTIDDTIQARQFFQTWKEKVFPQKPKAILCISAHYDTTYPTVNVV 60
           MALKDTFYVSHGSPTLTIDDTI+ARQFFQ+WKEK+FPQ+PKAILCISAHYDT+YPT+NVV
Sbjct: 1   MALKDTFYVSHGSPTLTIDDTIKARQFFQSWKEKIFPQRPKAILCISAHYDTSYPTINVV 60

Query: 61  SGPSDTIYDFYGFPSEMYKVNLTKISGSGAAVLAKKVKEALIGAGFEGVEEDKGRGLDHG 120
           SGP+DTIYDFYGFPS+MYK+   K    G A LAK+VKEALIGAGFE V+E+KGRGLDHG
Sbjct: 61  SGPNDTIYDFYGFPSKMYKL---KYPAPGGATLAKRVKEALIGAGFERVDEEKGRGLDHG 120

Query: 121 AWVPLMFMYPEADIPVCQLS---------------ALAFLKDEGVLIFGSGSATHNLRTL 180
           AWVPLMFMYPEADI VCQLS               ALA LKDEGVLIFGSGSATHNLRTL
Sbjct: 121 AWVPLMFMYPEADILVCQLSVQTRQNGIHHYNMGKALAPLKDEGVLIFGSGSATHNLRTL 180

Query: 181 DFDARSRATASWAIDFDKWLQQTLLQGRYEDVNEYEKKAPNPKMAHPSPDHFYPLHVAIG 240
           DFDAR  A ASWAIDFDKWLQQ LLQGRYEDVNEYEKKAP  KM HP+PDHF+PLHVAIG
Sbjct: 181 DFDARGSANASWAIDFDKWLQQALLQGRYEDVNEYEKKAPQAKMTHPNPDHFFPLHVAIG 240

Query: 241 AAGGQPKAKLIHHSWDLGTMSYASYQFTS 255
           AAGGQPKAKLIHHSWDLGTMSYASYQFT+
Sbjct: 241 AAGGQPKAKLIHHSWDLGTMSYASYQFTA 266

BLAST of PI0018885 vs. NCBI nr
Match: XP_022149362.1 (extradiol ring-cleavage dioxygenase [Momordica charantia])

HSP 1 Score: 419.5 bits (1077), Expect = 2.1e-113
Identity = 204/270 (75.56%), Postives = 227/270 (84.07%), Query Frame = 0

Query: 1   MALKDTFYVSHGSPTLTIDDTIQARQFFQTWKEKVFPQKPKAILCISAHYDTTYPTVNVV 60
           MA+ DTFY+SHGSPTL+IDDTI+ARQFFQ+WK+KVFPQKPK+ILCISAHYDT++PT+NVV
Sbjct: 1   MAVSDTFYISHGSPTLSIDDTIEARQFFQSWKQKVFPQKPKSILCISAHYDTSFPTLNVV 60

Query: 61  SGPSDTIYDFYGFPSEMYKVNLTKISGSGAAVLAKKVKEALIGAGFEGVEEDKGRGLDHG 120
           SGP+DTIYDFYGFPS MYK+   K    GA  LAK+VKEAL+GAGFE VEE++GRGLDHG
Sbjct: 61  SGPNDTIYDFYGFPSSMYKL---KYPAPGAPALAKRVKEALVGAGFERVEEERGRGLDHG 120

Query: 121 AWVPLMFMYPEADIPVCQLS---------------ALAFLKDEGVLIFGSGSATHNLRTL 180
           AWVPLMFMYPEADIPVCQLS               ALA LKDEGVLI  SGSATHNLRTL
Sbjct: 121 AWVPLMFMYPEADIPVCQLSVQSRKNGTYHYNLGRALAPLKDEGVLIVASGSATHNLRTL 180

Query: 181 DFDARSRATASWAIDFDKWLQQTLLQGRYEDVNEYEKKAPNPKMAHPSPDHFYPLHVAIG 240
           D  A+S   A WA++FD WL+  LLQGRYEDVNEYEKKAP+ KMAHPSPDH YPLHVA+G
Sbjct: 181 DRGAKS--IAPWALEFDNWLKDALLQGRYEDVNEYEKKAPHAKMAHPSPDHLYPLHVAMG 240

Query: 241 AAGGQPKAKLIHHSWDLGTMSYASYQFTSP 256
           AAGG  KAKLIHHSWDLGTMSYASYQFT+P
Sbjct: 241 AAGGNAKAKLIHHSWDLGTMSYASYQFTAP 265

BLAST of PI0018885 vs. NCBI nr
Match: KAG6603159.1 (Extradiol ring-cleavage dioxygenase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 415.2 bits (1066), Expect = 4.0e-112
Identity = 202/269 (75.09%), Postives = 223/269 (82.90%), Query Frame = 0

Query: 1   MALKDTFYVSHGSPTLTIDDTIQARQFFQTWKEKVFPQKPKAILCISAHYDTTYPTVNVV 60
           MAL DTFYVSHGSPTLTIDD I+AR FF++WK+KVF Q PKAILC+SAH+DT YPTVNVV
Sbjct: 1   MALNDTFYVSHGSPTLTIDDGIKARHFFKSWKDKVFSQTPKAILCVSAHFDTAYPTVNVV 60

Query: 61  SGPSDTIYDFYGFPSEMYKVNLTKISGSGAAVLAKKVKEALIGAGFEGVEEDKGRGLDHG 120
           SGP+DTIYDFYGFPS MY++   K    GA  LAK+VKEAL+GAGFE V+E+KGRGLDHG
Sbjct: 61  SGPNDTIYDFYGFPSSMYEL---KYPAPGAPTLAKRVKEALMGAGFERVDEEKGRGLDHG 120

Query: 121 AWVPLMFMYPEADIPVCQLS---------------ALAFLKDEGVLIFGSGSATHNLRTL 180
           AWVPLMFMYPEADIPVCQLS               ALA LKDEGVLI GSGSATHNLRTL
Sbjct: 121 AWVPLMFMYPEADIPVCQLSVQSRLNGTHHYNVGKALAPLKDEGVLIVGSGSATHNLRTL 180

Query: 181 DFDARSRATASWAIDFDKWLQQTLLQGRYEDVNEYEKKAPNPKMAHPSPDHFYPLHVAIG 240
           D  ARS   ASWAI+FD WL+  LLQGRY+DVNEY+KKAPN + AHPSPDH +PLHVAIG
Sbjct: 181 DRHARSSVNASWAIEFDDWLKHALLQGRYKDVNEYKKKAPNAEKAHPSPDHLFPLHVAIG 240

Query: 241 AAGGQPKAKLIHHSWDLGTMSYASYQFTS 255
           AAGG PKAKL+HHSWDLGTMSYASYQFT+
Sbjct: 241 AAGGHPKAKLVHHSWDLGTMSYASYQFTA 266

BLAST of PI0018885 vs. NCBI nr
Match: KAG7033478.1 (Extradiol ring-cleavage dioxygenase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 415.2 bits (1066), Expect = 4.0e-112
Identity = 202/269 (75.09%), Postives = 223/269 (82.90%), Query Frame = 0

Query: 1   MALKDTFYVSHGSPTLTIDDTIQARQFFQTWKEKVFPQKPKAILCISAHYDTTYPTVNVV 60
           MAL DTFYVSHGSPTLTIDD I+AR FF++WK+KVF Q PKAILC+SAH+DT YPTVNVV
Sbjct: 142 MALNDTFYVSHGSPTLTIDDGIKARHFFKSWKDKVFSQTPKAILCVSAHFDTAYPTVNVV 201

Query: 61  SGPSDTIYDFYGFPSEMYKVNLTKISGSGAAVLAKKVKEALIGAGFEGVEEDKGRGLDHG 120
           SGP+DTIYDFYGFPS MY++   K    GA  LAK+VKEAL+GAGFE V+E+KGRGLDHG
Sbjct: 202 SGPNDTIYDFYGFPSSMYEL---KYPAPGAPTLAKRVKEALMGAGFERVDEEKGRGLDHG 261

Query: 121 AWVPLMFMYPEADIPVCQLS---------------ALAFLKDEGVLIFGSGSATHNLRTL 180
           AWVPLMFMYPEADIPVCQLS               ALA LKDEGVLI GSGSATHNLRTL
Sbjct: 262 AWVPLMFMYPEADIPVCQLSVQSRLNGTHHYNVGKALAPLKDEGVLIVGSGSATHNLRTL 321

Query: 181 DFDARSRATASWAIDFDKWLQQTLLQGRYEDVNEYEKKAPNPKMAHPSPDHFYPLHVAIG 240
           D  ARS   ASWAI+FD WL+  LLQGRY+DVNEY+KKAPN + AHPSPDH +PLHVAIG
Sbjct: 322 DRHARSSVNASWAIEFDDWLKHALLQGRYKDVNEYKKKAPNAEKAHPSPDHLFPLHVAIG 381

Query: 241 AAGGQPKAKLIHHSWDLGTMSYASYQFTS 255
           AAGG PKAKL+HHSWDLGTMSYASYQFT+
Sbjct: 382 AAGGHPKAKLVHHSWDLGTMSYASYQFTA 407

BLAST of PI0018885 vs. NCBI nr
Match: XP_022933118.1 (extradiol ring-cleavage dioxygenase-like [Cucurbita moschata])

HSP 1 Score: 415.2 bits (1066), Expect = 4.0e-112
Identity = 202/269 (75.09%), Postives = 222/269 (82.53%), Query Frame = 0

Query: 1   MALKDTFYVSHGSPTLTIDDTIQARQFFQTWKEKVFPQKPKAILCISAHYDTTYPTVNVV 60
           MA  DTFYVSHGSPTLTIDD I+AR FF++WK+KVF Q PKAILC+SAH+DT YPTVNVV
Sbjct: 1   MAFNDTFYVSHGSPTLTIDDGIKARHFFKSWKDKVFSQTPKAILCVSAHFDTAYPTVNVV 60

Query: 61  SGPSDTIYDFYGFPSEMYKVNLTKISGSGAAVLAKKVKEALIGAGFEGVEEDKGRGLDHG 120
           SGP+DTIYDFYGFPS MY++   K    GA  LAK+VKEAL+GAGFE V+E+KGRGLDHG
Sbjct: 61  SGPNDTIYDFYGFPSSMYEL---KYPAPGAPALAKRVKEALMGAGFERVDEEKGRGLDHG 120

Query: 121 AWVPLMFMYPEADIPVCQLS---------------ALAFLKDEGVLIFGSGSATHNLRTL 180
           AWVPLMFMYPEADIPVCQLS               ALA LKDEGVLI GSGSATHNLRTL
Sbjct: 121 AWVPLMFMYPEADIPVCQLSVQSRLNGTHHYNVGKALAPLKDEGVLIVGSGSATHNLRTL 180

Query: 181 DFDARSRATASWAIDFDKWLQQTLLQGRYEDVNEYEKKAPNPKMAHPSPDHFYPLHVAIG 240
           D  ARS   ASWAI+FD WL+  LLQGRYEDVNEY+KKAPN + AHPSPDH +PLHVAIG
Sbjct: 181 DRHARSSVNASWAIEFDDWLKHALLQGRYEDVNEYKKKAPNAEKAHPSPDHLFPLHVAIG 240

Query: 241 AAGGQPKAKLIHHSWDLGTMSYASYQFTS 255
           AAGG PKAKL+HHSWDLGTMSYASYQFT+
Sbjct: 241 AAGGHPKAKLVHHSWDLGTMSYASYQFTA 266

BLAST of PI0018885 vs. TAIR 10
Match: AT4G15093.1 (catalytic LigB subunit of aromatic ring-opening dioxygenase family )

HSP 1 Score: 325.5 bits (833), Expect = 3.9e-89
Identity = 162/268 (60.45%), Postives = 198/268 (73.88%), Query Frame = 0

Query: 3   LKDTFYVSHGSPTLTIDDTIQARQFFQTWKEKVFPQKPKAILCISAHYDTTYPTVNVVSG 62
           +  TF++SHGSPTL+IDD+++ARQFF++W +KV PQKPK+IL ISAH+DT +P+VN V  
Sbjct: 4   VNQTFFLSHGSPTLSIDDSLEARQFFKSWTQKVLPQKPKSILVISAHWDTKFPSVNTVL- 63

Query: 63  PSDTIYDFYGFPSEMYKVNLTKISGSGAAVLAKKVKEALI-GAGFEGVEEDKGRGLDHGA 122
            ++TI+DF GFP  MYK+   K    GA  L K+VKE L+   G + V+ED  RGLDHGA
Sbjct: 64  RNNTIHDFSGFPDPMYKL---KYEAPGAIELGKRVKELLMKEGGMKRVDEDTKRGLDHGA 123

Query: 123 WVPLMFMYPEADIPVCQLS---------------ALAFLKDEGVLIFGSGSATHNLRTLD 182
           WVPLM MYPEADIP+CQLS               ALA LKDEGVLI GSGSATHNLR LD
Sbjct: 124 WVPLMLMYPEADIPICQLSVQSNQNGSYHYNMGKALASLKDEGVLIIGSGSATHNLRKLD 183

Query: 183 FDARSRATASWAIDFDKWLQQTLLQGRYEDVNEYEKKAPNPKMAHPSPDHFYPLHVAIGA 242
           F+    +   WA++FD WL+ +LLQGRY DVNE+E+KAPN KMAHP P+H YPLHV +GA
Sbjct: 184 FNITDGSPVPWALEFDHWLRDSLLQGRYGDVNEWEEKAPNAKMAHPWPEHLYPLHVVMGA 243

Query: 243 AGGQPKAKLIHHSWDLGTMSYASYQFTS 255
           AGG  KA+ IH SW LGT+SY+SY FTS
Sbjct: 244 AGGDAKAEQIHTSWQLGTLSYSSYSFTS 267

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q949R45.5e-8860.45Extradiol ring-cleavage dioxygenase OS=Arabidopsis thaliana OX=3702 GN=LIGB PE=2... [more]
Q70FG72.1e-7956.184,5-DOPA dioxygenase extradiol OS=Beta vulgaris OX=161934 GN=DODA PE=1 SV=1[more]
I3PFJ32.1e-7655.394,5-DOPA dioxygenase extradiol 1 OS=Beta vulgaris OX=161934 GN=DODA1 PE=2 SV=1[more]
I3PFJ91.4e-7554.654,5-DOPA dioxygenase extradiol 1 OS=Beta vulgaris OX=161934 GN=DODA1 PE=2 SV=1[more]
Q7XA486.5e-6548.154,5-DOPA dioxygenase extradiol OS=Portulaca grandiflora OX=3583 GN=DODA PE=1 SV=... [more]
Match NameE-valueIdentityDescription
A0A6J1D7R11.0e-11375.56extradiol ring-cleavage dioxygenase OS=Momordica charantia OX=3673 GN=LOC1110177... [more]
A0A6J1F3T41.9e-11275.09extradiol ring-cleavage dioxygenase-like OS=Cucurbita moschata OX=3662 GN=LOC111... [more]
A0A6J1HS492.8e-11174.35extradiol ring-cleavage dioxygenase-like OS=Cucurbita maxima OX=3661 GN=LOC11146... [more]
A0A6J1HWD71.8e-11073.98extradiol ring-cleavage dioxygenase-like OS=Cucurbita maxima OX=3661 GN=LOC11146... [more]
A0A5A7TUJ33.1e-11073.51Extradiol ring-cleavage dioxygenase OS=Cucumis melo var. makuwa OX=1194695 GN=E6... [more]
Match NameE-valueIdentityDescription
XP_038883407.12.3e-12381.78extradiol ring-cleavage dioxygenase-like [Benincasa hispida][more]
XP_022149362.12.1e-11375.56extradiol ring-cleavage dioxygenase [Momordica charantia][more]
KAG6603159.14.0e-11275.09Extradiol ring-cleavage dioxygenase, partial [Cucurbita argyrosperma subsp. soro... [more]
KAG7033478.14.0e-11275.09Extradiol ring-cleavage dioxygenase, partial [Cucurbita argyrosperma subsp. argy... [more]
XP_022933118.14.0e-11275.09extradiol ring-cleavage dioxygenase-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
AT4G15093.13.9e-8960.45catalytic LigB subunit of aromatic ring-opening dioxygenase family [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.40.830.10coord: 2..253
e-value: 1.7E-68
score: 232.4
NoneNo IPR availablePANTHERPTHR30096:SF18EXTRADIOL RING-CLEAVAGE DIOXYGENASE-LIKEcoord: 3..254
NoneNo IPR availablePANTHERPTHR30096UNCHARACTERIZEDcoord: 3..254
NoneNo IPR availableSUPERFAMILY53213LigB-likecoord: 6..252
IPR004183Extradiol ring-cleavage dioxygenase, class III enzyme, subunit BPFAMPF02900LigBcoord: 147..252
e-value: 4.1E-20
score: 72.0
coord: 6..141
e-value: 5.2E-24
score: 84.8
IPR014436Extradiol aromatic ring-opening dioxygenase, DODA-typePIRSFPIRSF006157Doxgns_DODAcoord: 1..254
e-value: 3.5E-76
score: 253.8
IPR014436Extradiol aromatic ring-opening dioxygenase, DODA-typeCDDcd0736345_DOPA_Dioxygenasecoord: 6..252
e-value: 1.09465E-100
score: 291.738

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0018885.1PI0018885.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006725 cellular aromatic compound metabolic process
molecular_function GO:0051213 dioxygenase activity
molecular_function GO:0008198 ferrous iron binding
molecular_function GO:0016701 oxidoreductase activity, acting on single donors with incorporation of molecular oxygen
molecular_function GO:0008270 zinc ion binding
molecular_function GO:0016491 oxidoreductase activity