PI0018013 (gene) Melon (PI 482460) v1

BLAST of PI0018013 vs. ExPASy Swiss-Prot
Match: M4IQQ5 (2-methylpropanoate--CoA ligase CCL4 OS=Humulus lupulus OX=3486 GN=CCL4 PE=1 SV=1)

HSP 1 Score: 827.0 bits (2135), Expect = 1.3e-238
Identity = 382/552 (69.20%), Postives = 469/552 (84.96%), Query Frame = 0

Query: 1   MEDLKPRPANSFPLTPIGFIERAAIIYGDSTSIIYNSTTYTWSETHRRCLRLASSISSFG 60
           MEDLKPRPA+S PLTP+GF+ERAA +YGD TS++Y++ +YTWS+THRRCL LASSI+S G
Sbjct: 1   MEDLKPRPASSSPLTPLGFLERAATVYGDCTSVVYDAVSYTWSQTHRRCLCLASSIASLG 60

Query: 61  IQRGHVVSVIAPNTPAMYELHFAVPMVGAILNNINLRLDTRTISILLRHSESKLIFVDQA 120
           I+ GHVVSV+APN P MYELHFAVPM GAILN +NLRLD RTISILL HSESKLIFVD  
Sbjct: 61  IENGHVVSVLAPNVPQMYELHFAVPMAGAILNAVNLRLDARTISILLHHSESKLIFVDHL 120

Query: 121 SCAVIFEALALFPPEMKRPLLVLIADDTVEQDSTLPSFVSIRDNFVDSYEDMVKRGDSKF 180
           S  +I EA+ALFP +   P LV +AD++   +S+      +   F  SY+D++ RGD  F
Sbjct: 121 SRDLILEAIALFPKQAPVPRLVFMADESESGNSS-----ELGKEFFCSYKDLIDRGDPDF 180

Query: 181 QWMRPLSEWDPIVLNYTSGTTSSPKGVVHCHRGIFTVTLDSLLEWGVPKQSVYLWTLPMF 240
           +W+ P SEWDP++LNYTSGTTSSPKGVVHCHRGIF +T+DSL++WGVPKQ VYLWTLPMF
Sbjct: 181 KWVMPKSEWDPMILNYTSGTTSSPKGVVHCHRGIFIMTVDSLIDWGVPKQPVYLWTLPMF 240

Query: 241 HGNGWSFPWGMAAVGGTNVCIRKFDATLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNLPL 300
           H NGWS+PWGMAAVGGTN+C+RKFD+ +I+ +I+RH VTH+CGAPVVLNML+N+P + PL
Sbjct: 241 HANGWSYPWGMAAVGGTNICLRKFDSEIIYDMIKRHGVTHMCGAPVVLNMLSNAPGSEPL 300

Query: 301 DRPVKILTAGAPPPASILLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAIEQAR 360
              V+I+TAGAPPP+++L RTESLGF V+HGYGLTE+AG ++ C+WK EWN LPA E+AR
Sbjct: 301 KTTVQIMTAGAPPPSAVLFRTESLGFAVSHGYGLTETAGLVVSCAWKKEWNHLPATERAR 360

Query: 361 LKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRGGSVMLGYLKDPEGTTKAMSD 420
           LK+RQGV  +    +DV+DP T  +VKRDG+++GE+V+RGGSVMLGYLKDPEGT K+M+ 
Sbjct: 361 LKSRQGVGTVMQTKIDVVDPVTGAAVKRDGSTLGEVVLRGGSVMLGYLKDPEGTAKSMTA 420

Query: 421 DGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPMVNEAAVVARPD 480
           DGWF+TGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVES+LY++P + EAAVVARPD
Sbjct: 421 DGWFYTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESILYSHPDILEAAVVARPD 480

Query: 481 EFWGETPCAFISLKEELTRKPTEAEIIEYCKGKLPRFMVPKTVIFMTEMPKTATGKIQKF 540
           EFWGETPCAF+SLK+ LT+KPTE EI+EYC+ KLPR+MVPKTV+F  E+PKT+TGK+QKF
Sbjct: 481 EFWGETPCAFVSLKKGLTKKPTEKEIVEYCRSKLPRYMVPKTVVFKEELPKTSTGKVQKF 540

Query: 541 VLRQMAKSLSPA 553
           +LR MA+ +  A
Sbjct: 541 ILRDMARGMGSA 547

BLAST of PI0018013 vs. ExPASy Swiss-Prot
Match: M4ISH2 (Probable CoA ligase CCL11 OS=Humulus lupulus OX=3486 GN=CCL11 PE=2 SV=1)

HSP 1 Score: 769.2 bits (1985), Expect = 3.1e-221
Identity = 359/557 (64.45%), Postives = 455/557 (81.69%), Query Frame = 0

Query: 1   MEDLKPRPANSFPLTPIGFIERAAIIYGDSTSIIYNSTTYTWSETHRRCLRLASSISS-F 60
           ME+LKPR ANS PLTP+GF+ERAA +YGD  S++Y+  +YTWS+TH RCLR+AS I S  
Sbjct: 1   MEELKPRFANSCPLTPLGFLERAATVYGDCISVVYDDLSYTWSQTHTRCLRVASCIESCL 60

Query: 61  GIQRGHVVSVIAPNTPAMYELHFAVPMVGAILNNINLRLDTRTISILLRHSESKLIFVDQ 120
           G+++G VVSV+APN PAMYEL+FAVPM G +LNNIN RL+  TIS++LRHSESKL+FVDQ
Sbjct: 61  GVKKGQVVSVVAPNVPAMYELNFAVPMAGVVLNNINTRLNAVTISVMLRHSESKLVFVDQ 120

Query: 121 ASCAVIFEALALFPPEMKRPLLVLIADDTVEQDSTLPSFVSIRDNFVDSYEDMVKRGDSK 180
            S  ++ +A++LFP     PLLVLIAD+   ++ T    V  R++FV  YED+V++GD K
Sbjct: 121 LSVRLVLDAVSLFPKNTPTPLLVLIADNITGENLT----VEDREHFVCCYEDLVEKGDDK 180

Query: 181 -FQWMRPLSEWDPIVLNYTSGTTSSPKGVVHCHRGIFTVTLDSLLEWGVPKQSVYLWTLP 240
            F+W+RP+SEWDPIVLNYTSGTTSSPKGVVH HR +F VTLDSL++WGVPKQ VYLWTLP
Sbjct: 181 TFKWVRPISEWDPIVLNYTSGTTSSPKGVVHSHRSVFVVTLDSLIDWGVPKQPVYLWTLP 240

Query: 241 MFHGNGWSFPWGMAAVGGTNVCIRKFDATLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNL 300
           MFH NGW + WG+AAVGGTN+C+R+FD  +IF+LIRRH VTH+C AP+VLNML+NSP+  
Sbjct: 241 MFHANGWGYTWGIAAVGGTNICLRRFDGEIIFNLIRRHRVTHMCAAPIVLNMLSNSPNAE 300

Query: 301 PLDRPVKILTAGAPPPASILLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAIEQ 360
           PL  PV ++T GAPPPA++LLRTESLGFV++HGYG+TE  G ++ C+WK EWN+LPA EQ
Sbjct: 301 PLPNPVHVMTGGAPPPAAVLLRTESLGFVISHGYGMTEMLGVVVSCAWKREWNRLPATEQ 360

Query: 361 ARLKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRGGSVMLGYLKDPEGTTKAM 420
           ARLK+RQGVR  A+  VDV+DP +  SVKRDG ++GEIV++G S+MLGYLK+   T K +
Sbjct: 361 ARLKSRQGVRTAAMMEVDVVDPNSGVSVKRDGLTMGEIVLKGSSIMLGYLKNSAATAKCI 420

Query: 421 SDDGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPMVNEAAVVAR 480
             DGWF+TGD+ VMHPDGYLEIKDRSKDVIISGGEN+SSVEVES LY++P V+EAAVVA 
Sbjct: 421 RADGWFYTGDMAVMHPDGYLEIKDRSKDVIISGGENVSSVEVESALYSHPAVDEAAVVAC 480

Query: 481 PDEFWGETPCAFISLKEELTRKPTEAEIIEYCKGKLPRFMVPKTVIFMTEMPKTATGKIQ 540
           PDE+WGETP AF++LK+ +  +PTE EI+EYC+ KL  FMVPK V+F  ++PKT+TGKIQ
Sbjct: 481 PDEYWGETPFAFVTLKKGMRVRPTEKEILEYCREKLAHFMVPKVVVFRDKLPKTSTGKIQ 540

Query: 541 KFVLRQMAKSLSPAQKS 556
           KF L+++ K++  +  S
Sbjct: 541 KFTLKEIVKTMGYSSSS 553

BLAST of PI0018013 vs. ExPASy Swiss-Prot
Match: Q9FFE6 (Probable acyl-activating enzyme 5, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=AAE5 PE=1 SV=1)

HSP 1 Score: 765.4 bits (1975), Expect = 4.5e-220
Identity = 365/558 (65.41%), Postives = 459/558 (82.26%), Query Frame = 0

Query: 1   MEDLKPRPANSFPLTPIGFIERAAIIYGDSTSIIYNS-TTYTWSETHRRCLRLASSISSF 60
           ME +KP  ANS PLTPIGF+ERAA +YGD TSI+Y S T YTW ET+ RCLR+ASS+SS 
Sbjct: 1   MEQMKPCAANSPPLTPIGFLERAATVYGDCTSIVYGSNTVYTWRETNLRCLRVASSLSSI 60

Query: 61  GIQRGHVVSVIAPNTPAMYELHFAVPMVGAILNNINLRLDTRTISILLRHSESKLIFVDQ 120
           GI R  VVSV++PNTPAMYEL FAVPM GAILNNIN RLD RT+S+LLRH  SKL+FVD 
Sbjct: 61  GIGRSDVVSVLSPNTPAMYELQFAVPMSGAILNNINTRLDARTVSVLLRHCGSKLLFVDV 120

Query: 121 ASCAVIFEALALFPPEMKRPLLVLIADDTVEQDSTLPSFVSIRDNFVDSYEDMVKRGDSK 180
            S  +  EA+++   +   P+LV IAD   +++    + V+ R  F  +Y+D++ RGD  
Sbjct: 121 FSVDLAVEAISMMTTD--PPILVFIAD---KEEEGGDADVADRTKFSYTYDDLIHRGDLD 180

Query: 181 FQWMRPLSEWDPIVLNYTSGTTSSPKGVVHCHRGIFTVTLDSLLEWGVPKQSVYLWTLPM 240
           F+W+RP SEWDP+VLNYTSGTTS+PKGVVHCHRGIF +++DSL++W VPK  VYLWTLP+
Sbjct: 181 FKWIRPESEWDPVVLNYTSGTTSAPKGVVHCHRGIFVMSIDSLIDWTVPKNPVYLWTLPI 240

Query: 241 FHGNGWSFPWGMAAVGGTNVCIRKFDATLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNLP 300
           FH NGWS+PWG+AAVGGTNVC+RKFDA LI+ LIR H VTH+CGAPVVLNML+ + +  P
Sbjct: 241 FHANGWSYPWGIAAVGGTNVCLRKFDAPLIYRLIRDHGVTHMCGAPVVLNMLSATNEFQP 300

Query: 301 LDRPVKILTAGAPPPASILLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAIEQA 360
           L+RPV ILTAGAPPPA++LLR ES+GFV++HGYGLTE+AG  + C+WK +WN+LPA ++A
Sbjct: 301 LNRPVNILTAGAPPPAAVLLRAESIGFVISHGYGLTETAGLNVSCAWKPQWNRLPASDRA 360

Query: 361 RLKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRGGSVMLGYLKDPEGTTKAMS 420
           RLKARQGVR +    +DV+DPE+ +SV+R+G ++GEIV+RG S+MLGYLKDP GT KA+ 
Sbjct: 361 RLKARQGVRTVGFTEIDVVDPESGRSVERNGETVGEIVMRGSSIMLGYLKDPVGTEKALK 420

Query: 421 DDGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPMVNEAAVVARP 480
            +GWF+TGDVGV+H DGYLEIKDRSKD+II+GGEN+SSVEVE+VLYTNP VNE AVVARP
Sbjct: 421 -NGWFYTGDVGVIHSDGYLEIKDRSKDIIITGGENVSSVEVETVLYTNPAVNEVAVVARP 480

Query: 481 DEFWGETPCAFISLKEELTRKPTEAEIIEYCKGKLPRFMVPKTVIFMTEMPKTATGKIQK 540
           D FWGETPCAF+SLK  LT++PTE E+IEYC+ K+P++MVPKTV F+ E+PKT+TGK+ K
Sbjct: 481 DVFWGETPCAFVSLKSGLTQRPTEVEMIEYCRKKMPKYMVPKTVSFVDELPKTSTGKVMK 540

Query: 541 FVLRQMAKSLSPAQKSRL 558
           FVLR++AK +   + SR+
Sbjct: 541 FVLREIAKKMGTTRLSRM 552

BLAST of PI0018013 vs. ExPASy Swiss-Prot
Match: Q9FFE9 (Probable acyl-activating enzyme 6 OS=Arabidopsis thaliana OX=3702 GN=AAE6 PE=2 SV=1)

HSP 1 Score: 755.0 bits (1948), Expect = 6.1e-217
Identity = 360/550 (65.45%), Postives = 452/550 (82.18%), Query Frame = 0

Query: 1   MEDLKPRPANSFPLTPIGFIERAAIIYGDSTSIIYNS-TTYTWSETHRRCLRLASSISSF 60
           ME++KP  ANS PLTPIGF+ERAA +YGD TSI+Y S T YTW ET+ RCLR+ASS+SS 
Sbjct: 1   MEEMKPCAANSPPLTPIGFLERAATVYGDCTSIVYGSNTVYTWRETNLRCLRVASSLSSI 60

Query: 61  GIQRGHVVSVIAPNTPAMYELHFAVPMVGAILNNINLRLDTRTISILLRHSESKLIFVDQ 120
           GI R  VVSV++PNTPAMYEL FAVPM GAILNNIN RLD RT+S+LLRH ESKL+FVD 
Sbjct: 61  GIGRSDVVSVLSPNTPAMYELQFAVPMSGAILNNINTRLDARTVSVLLRHCESKLLFVDV 120

Query: 121 ASCAVIFEALALFPPEMKRPLLVLIADDTVEQDSTLPSFVSIRDNFVDSYEDMVKRGDSK 180
            S  +  EA+++   +   P+LV+IAD   E++  +     +   F  +Y+D+++RGD  
Sbjct: 121 FSVDLAVEAVSMMTTD--PPILVVIADK--EEEGGVADVADL-SKFSYTYDDLIERGDPG 180

Query: 181 FQWMRPLSEWDPIVLNYTSGTTSSPKGVVHCHRGIFTVTLDSLLEWGVPKQSVYLWTLPM 240
           F+W+RP SEWDP+VLNYTSGTTS+PKGVVHCHRGIF +++DSL++W VPK  VYLWTLP+
Sbjct: 181 FKWIRPESEWDPVVLNYTSGTTSAPKGVVHCHRGIFVMSVDSLIDWAVPKNPVYLWTLPI 240

Query: 241 FHGNGWSFPWGMAAVGGTNVCIRKFDATLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNLP 300
           FH NGW+ PWG+AAVGGTNVC+RKFDA LI+ LIR H VTH+CGAPVVLNML+ + ++ P
Sbjct: 241 FHSNGWTNPWGIAAVGGTNVCLRKFDAPLIYRLIRDHGVTHMCGAPVVLNMLSATQESQP 300

Query: 301 LDRPVKILTAGAPPPASILLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAIEQA 360
           L+ PV ILTAG+PPPA++LLR ES+GFV++HGYGLTE+AG I+ C+WK +WN LPA ++A
Sbjct: 301 LNHPVNILTAGSPPPATVLLRAESIGFVISHGYGLTETAGVIVSCAWKPKWNHLPASDRA 360

Query: 361 RLKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRGGSVMLGYLKDPEGTTKAMS 420
           RLKARQGVR +    +DV+DPE+  SV+R+G ++GEIV+RG SVMLGYLKDP GT KA+ 
Sbjct: 361 RLKARQGVRTVGFTEIDVVDPESGLSVERNGETVGEIVMRGSSVMLGYLKDPVGTEKALK 420

Query: 421 DDGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPMVNEAAVVARP 480
            +GWF+TGDVGV+H DGYLEIKDRSKD+II+GGEN+SSVEVE+VLYT P VNE AVVARP
Sbjct: 421 -NGWFYTGDVGVIHSDGYLEIKDRSKDIIITGGENVSSVEVETVLYTIPAVNEVAVVARP 480

Query: 481 DEFWGETPCAFISLKEELTRKPTEAEIIEYCKGKLPRFMVPKTVIFMTEMPKTATGKIQK 540
           DEFWGETPCAF+SLK   + KPTE E++EYC+ K+P++MVPKTV FM E+PK++TGK+ K
Sbjct: 481 DEFWGETPCAFVSLKNGFSGKPTEEELMEYCRKKMPKYMVPKTVSFMDELPKSSTGKVTK 540

Query: 541 FVLRQMAKSL 550
           FVLR +AK +
Sbjct: 541 FVLRDIAKKM 544

BLAST of PI0018013 vs. ExPASy Swiss-Prot
Match: Q9LQS1 (Probable acyl-activating enzyme 8 OS=Arabidopsis thaliana OX=3702 GN=AAE8 PE=2 SV=1)

HSP 1 Score: 730.3 bits (1884), Expect = 1.6e-209
Identity = 355/551 (64.43%), Postives = 441/551 (80.04%), Query Frame = 0

Query: 1   MEDLKPRPANSFPLTPIGFIERAAIIYGDSTSIIY-NSTTYTWSETHRRCLRLASSISSF 60
           MEDLKP  ANS PLT +GF+ERAA +YGD TSI+Y NST YTW ET+ RCL +AS++SS 
Sbjct: 1   MEDLKPSAANSLPLTLLGFLERAATVYGDCTSIVYGNSTVYTWRETNHRCLCVASALSSI 60

Query: 61  GIQRGHVVSVIAPNTPAMYELHFAVPMVGAILNNINLRLDTRTISILLRHSESKLIFVDQ 120
           GI R  VVSV++ NTP MYEL F+VPM GAILNNIN RLD RT+S+LLRH ESKL+FVD 
Sbjct: 61  GIGRSDVVSVLSANTPEMYELQFSVPMSGAILNNINTRLDARTVSVLLRHCESKLLFVDF 120

Query: 121 ASCAVIFEALALFPPEMKRPLLVLIADDTVEQDSTLPSFVSIRDNFVDSYEDMVKRGDSK 180
               +  EA+ +    +  P+LVLIA++  E+     + V+ R  F   Y D++ RG+  
Sbjct: 121 FYSDLAVEAITML---LNPPILVLIANEEEEEGG---AEVTERSKFCYLYSDLITRGNPD 180

Query: 181 FQWMRPLSEWDPIVLNYTSGTTSSPKGVVHCHRGIFTVTLDSLLEWGVPKQSVYLWTLPM 240
           F+W+RP SEWDPIV+NYTSGTTSSPKGVVHCHRGIF +TLDSL +W VPK  VYLWTLP+
Sbjct: 181 FKWIRPGSEWDPIVVNYTSGTTSSPKGVVHCHRGIFVMTLDSLTDWAVPKTPVYLWTLPI 240

Query: 241 FHGNGWSFPWGMAAVGGTNVCIRKFDATLIFSLIRRHHVTHLCGAPVVLNMLTNSPD-NL 300
           FH NGW++PWG+AAVGGTNVC+RK  A  I+ LIR H VTH+ GAP+VL +L+ S + + 
Sbjct: 241 FHANGWTYPWGIAAVGGTNVCVRKLHAPSIYHLIRDHGVTHMYGAPIVLQILSASQESDQ 300

Query: 301 PLDRPVKILTAGAPPPASILLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAIEQ 360
           PL  PV  LTAG+ PPA++LLR ESLGF+V+HGYGLTE+AG I+ C+WK  WN+LPA +Q
Sbjct: 301 PLKSPVNFLTAGSSPPATVLLRAESLGFIVSHGYGLTETAGVIVSCAWKPNWNRLPASDQ 360

Query: 361 ARLKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRGGSVMLGYLKDPEGTTKAM 420
           A+LK+RQGVR +    +DV+DPE+ +SV+RDG ++GEIV+RG S+MLGYLK+P GT  + 
Sbjct: 361 AQLKSRQGVRTVGFSEIDVVDPESGRSVERDGETVGEIVLRGSSIMLGYLKNPIGTQNSF 420

Query: 421 SDDGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPMVNEAAVVAR 480
             +GWFFTGD+GV+H DGYLEIKDRSKDVIISGGEN+SSVEVE+VLYTNP VNEAAVVAR
Sbjct: 421 K-NGWFFTGDLGVIHGDGYLEIKDRSKDVIISGGENVSSVEVEAVLYTNPAVNEAAVVAR 480

Query: 481 PDEFWGETPCAFISLKEELTRKPTEAEIIEYCKGKLPRFMVPKTVIFMTEMPKTATGKIQ 540
           PDEFWGETPCAF+SLK  LTRKPT+ EIIEYCK K+PR+M PKTV F+ E+PKT+TGKI 
Sbjct: 481 PDEFWGETPCAFVSLKPGLTRKPTDKEIIEYCKYKMPRYMAPKTVSFLEELPKTSTGKII 540

Query: 541 KFVLRQMAKSL 550
           K +L+++AK++
Sbjct: 541 KSLLKEIAKNM 544

BLAST of PI0018013 vs. ExPASy TrEMBL
Match: A0A5D3D623 (Putative acyl-activating enzyme 5, peroxisomal OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold418G00690 PE=4 SV=1)

HSP 1 Score: 1078.9 bits (2789), Expect = 0.0e+00
Identity = 533/557 (95.69%), Postives = 541/557 (97.13%), Query Frame = 0

Query: 1   MEDLKPRPANSFPLTPIGFIERAAIIYGDSTSIIYNSTTYTWSETHRRCLRLASSISSFG 60
           MEDLKP PANSFPLTPIGFI+RAA IYGDSTSIIYNSTTYTWSETHRRCL+LASSISSFG
Sbjct: 1   MEDLKPTPANSFPLTPIGFIQRAATIYGDSTSIIYNSTTYTWSETHRRCLQLASSISSFG 60

Query: 61  IQRGHVVSVIAPNTPAMYELHFAVPMVGAILNNINLRLDTRTISILLRHSESKLIFVDQA 120
           IQRGHVVSVIAPNTPAMYELHFAVPMVGAILN INLRLDTRTISILLRHSESKLIFVDQA
Sbjct: 61  IQRGHVVSVIAPNTPAMYELHFAVPMVGAILNTINLRLDTRTISILLRHSESKLIFVDQA 120

Query: 121 SCAVIFEALALFPPEMKRPLLVLIADDTVEQDSTLPSFVSIRDNFVDSYEDMVKRGDSKF 180
           SCA+IFEALALFPPEMKRPLLVLIADD  EQDS+LPSFVSIRDNFVDSYEDMVKRGDSKF
Sbjct: 121 SCALIFEALALFPPEMKRPLLVLIADDAAEQDSSLPSFVSIRDNFVDSYEDMVKRGDSKF 180

Query: 181 QWMRPLSEWDPIVLNYTSGTTSSPKGVVHCHRGIFTVTLDSLLEWGVPKQSVYLWTLPMF 240
           QW+RPLSEWDPIVLNYTSGTTSSPKGVVHCHRGIFTV LDSLLEWGVPKQSVYLWTLPMF
Sbjct: 181 QWIRPLSEWDPIVLNYTSGTTSSPKGVVHCHRGIFTVALDSLLEWGVPKQSVYLWTLPMF 240

Query: 241 HGNGWSFPWGMAAVGGTNVCIRKFDATLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNLPL 300
           HGNGWSFPWG+AAVGGTNVCIRKFDA LIFSLIRRHHVTHLCGAPVVLNMLTNSPDNLPL
Sbjct: 241 HGNGWSFPWGIAAVGGTNVCIRKFDARLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNLPL 300

Query: 301 DRPVKILTAGAPPPASILLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAIEQAR 360
           DRPVKILTAGAPPPASILLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAIEQAR
Sbjct: 301 DRPVKILTAGAPPPASILLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAIEQAR 360

Query: 361 LKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRGGSVMLGYLKDPEGTTKAMSD 420
           LKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRG SV LGYLKDPEGT KAMS+
Sbjct: 361 LKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRGASVTLGYLKDPEGTAKAMSE 420

Query: 421 DGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPMVNEAAVVARPD 480
           DGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLY NPMVNEAAVVARPD
Sbjct: 421 DGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYMNPMVNEAAVVARPD 480

Query: 481 EFWGETPCAFISLKEELTRKPTEAEIIEYCKGKLPRFMVPKTVIFMTEMPKTATGKIQKF 540
           EFWGETPCAFISLKE L+RKPTE EIIEYCKGKLPRFMVPKTVIFMTEMPKTATGKIQKF
Sbjct: 481 EFWGETPCAFISLKEGLSRKPTEEEIIEYCKGKLPRFMVPKTVIFMTEMPKTATGKIQKF 540

Query: 541 VLRQMAKSLSPAQKSRL 558
           VLRQ AKSL PA KSRL
Sbjct: 541 VLRQKAKSLGPAPKSRL 557

BLAST of PI0018013 vs. ExPASy TrEMBL
Match: A0A1S3CIL0 (probable acyl-activating enzyme 5, peroxisomal OS=Cucumis melo OX=3656 GN=LOC103501381 PE=4 SV=1)

HSP 1 Score: 1078.9 bits (2789), Expect = 0.0e+00
Identity = 533/557 (95.69%), Postives = 541/557 (97.13%), Query Frame = 0

Query: 1   MEDLKPRPANSFPLTPIGFIERAAIIYGDSTSIIYNSTTYTWSETHRRCLRLASSISSFG 60
           MEDLKP PANSFPLTPIGFI+RAA IYGDSTSIIYNSTTYTWSETHRRCL+LASSISSFG
Sbjct: 1   MEDLKPTPANSFPLTPIGFIQRAATIYGDSTSIIYNSTTYTWSETHRRCLQLASSISSFG 60

Query: 61  IQRGHVVSVIAPNTPAMYELHFAVPMVGAILNNINLRLDTRTISILLRHSESKLIFVDQA 120
           IQRGHVVSVIAPNTPAMYELHFAVPMVGAILN INLRLDTRTISILLRHSESKLIFVDQA
Sbjct: 61  IQRGHVVSVIAPNTPAMYELHFAVPMVGAILNTINLRLDTRTISILLRHSESKLIFVDQA 120

Query: 121 SCAVIFEALALFPPEMKRPLLVLIADDTVEQDSTLPSFVSIRDNFVDSYEDMVKRGDSKF 180
           SCA+IFEALALFPPEMKRPLLVLIADD  EQDS+LPSFVSIRDNFVDSYEDMVKRGDSKF
Sbjct: 121 SCALIFEALALFPPEMKRPLLVLIADDAAEQDSSLPSFVSIRDNFVDSYEDMVKRGDSKF 180

Query: 181 QWMRPLSEWDPIVLNYTSGTTSSPKGVVHCHRGIFTVTLDSLLEWGVPKQSVYLWTLPMF 240
           QW+RPLSEWDPIVLNYTSGTTSSPKGVVHCHRGIFTV LDSLLEWGVPKQSVYLWTLPMF
Sbjct: 181 QWIRPLSEWDPIVLNYTSGTTSSPKGVVHCHRGIFTVALDSLLEWGVPKQSVYLWTLPMF 240

Query: 241 HGNGWSFPWGMAAVGGTNVCIRKFDATLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNLPL 300
           HGNGWSFPWG+AAVGGTNVCIRKFDA LIFSLIRRHHVTHLCGAPVVLNMLTNSPDNLPL
Sbjct: 241 HGNGWSFPWGIAAVGGTNVCIRKFDARLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNLPL 300

Query: 301 DRPVKILTAGAPPPASILLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAIEQAR 360
           DRPVKILTAGAPPPASILLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAIEQAR
Sbjct: 301 DRPVKILTAGAPPPASILLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAIEQAR 360

Query: 361 LKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRGGSVMLGYLKDPEGTTKAMSD 420
           LKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRG SV LGYLKDPEGT KAMS+
Sbjct: 361 LKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRGASVTLGYLKDPEGTAKAMSE 420

Query: 421 DGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPMVNEAAVVARPD 480
           DGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLY NPMVNEAAVVARPD
Sbjct: 421 DGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYMNPMVNEAAVVARPD 480

Query: 481 EFWGETPCAFISLKEELTRKPTEAEIIEYCKGKLPRFMVPKTVIFMTEMPKTATGKIQKF 540
           EFWGETPCAFISLKE L+RKPTE EIIEYCKGKLPRFMVPKTVIFMTEMPKTATGKIQKF
Sbjct: 481 EFWGETPCAFISLKEGLSRKPTEEEIIEYCKGKLPRFMVPKTVIFMTEMPKTATGKIQKF 540

Query: 541 VLRQMAKSLSPAQKSRL 558
           VLRQ AKSL PA KSRL
Sbjct: 541 VLRQKAKSLGPAPKSRL 557

BLAST of PI0018013 vs. ExPASy TrEMBL
Match: A0A0A0KMT3 (AMP dependent ligase OS=Cucumis sativus OX=3659 GN=Csa_5G318390 PE=4 SV=1)

HSP 1 Score: 1056.2 bits (2730), Expect = 4.7e-305
Identity = 517/557 (92.82%), Postives = 537/557 (96.41%), Query Frame = 0

Query: 1   MEDLKPRPANSFPLTPIGFIERAAIIYGDSTSIIYNSTTYTWSETHRRCLRLASSISSFG 60
           MEDLKPRPANSFPLTPIGFIERAAIIYGDSTSIIYNSTTYTWSETHRRCL+LASSISSFG
Sbjct: 1   MEDLKPRPANSFPLTPIGFIERAAIIYGDSTSIIYNSTTYTWSETHRRCLQLASSISSFG 60

Query: 61  IQRGHVVSVIAPNTPAMYELHFAVPMVGAILNNINLRLDTRTISILLRHSESKLIFVDQA 120
           IQRGHVVSV APNTPAMYELHFAVPM GAILN INLRLDTRTISILLRHSESKLIFVDQ 
Sbjct: 61  IQRGHVVSVFAPNTPAMYELHFAVPMAGAILNTINLRLDTRTISILLRHSESKLIFVDQP 120

Query: 121 SCAVIFEALALFPPEMKRPLLVLIADDTVEQDSTLPSFVSIRDNFVDSYEDMVKRGDSKF 180
           SCA+IF+ALALFPP+MKRPLLVLI DDT EQDS+LPS VSIRDNFVDSYE+MVK GDSKF
Sbjct: 121 SCALIFDALALFPPDMKRPLLVLITDDTAEQDSSLPSVVSIRDNFVDSYENMVKSGDSKF 180

Query: 181 QWMRPLSEWDPIVLNYTSGTTSSPKGVVHCHRGIFTVTLDSLLEWGVPKQSVYLWTLPMF 240
           QW+RPLSEWDPIVLNYTSGTTS+PKGVV+CHRG+FTV LDS+LEWGVPKQSVYLWTLPMF
Sbjct: 181 QWIRPLSEWDPIVLNYTSGTTSAPKGVVNCHRGVFTVALDSILEWGVPKQSVYLWTLPMF 240

Query: 241 HGNGWSFPWGMAAVGGTNVCIRKFDATLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNLPL 300
           HGNGWSFPWG+AAVGGTNVCIRKFD+ LIFSLIRRHHVTHLCGAPVVLNMLTNSPDNLPL
Sbjct: 241 HGNGWSFPWGIAAVGGTNVCIRKFDSRLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNLPL 300

Query: 301 DRPVKILTAGAPPPASILLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAIEQAR 360
           DRPVKILTAGAPPPAS+LLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAIEQAR
Sbjct: 301 DRPVKILTAGAPPPASVLLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAIEQAR 360

Query: 361 LKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRGGSVMLGYLKDPEGTTKAMSD 420
           LKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRG S+MLGYLKD E T+KAMS+
Sbjct: 361 LKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRGPSLMLGYLKDTEATSKAMSE 420

Query: 421 DGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPMVNEAAVVARPD 480
           DGWFFTGDVGVMHPDGY+EIKDRSKDVIISGGENLSSVEVESVLY NPMVNEAAVVARPD
Sbjct: 421 DGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPMVNEAAVVARPD 480

Query: 481 EFWGETPCAFISLKEELTRKPTEAEIIEYCKGKLPRFMVPKTVIFMTEMPKTATGKIQKF 540
           EFWGETPCAFISLKE L+RKPTE EIIEYCKGKLP+FMVPKTVIFMTEMPKTATGK QKF
Sbjct: 481 EFWGETPCAFISLKEGLSRKPTEEEIIEYCKGKLPKFMVPKTVIFMTEMPKTATGKFQKF 540

Query: 541 VLRQMAKSLSPAQKSRL 558
           VLRQ AKSL P QKSRL
Sbjct: 541 VLRQKAKSLGPPQKSRL 557

BLAST of PI0018013 vs. ExPASy TrEMBL
Match: A0A6J1FL76 (probable acyl-activating enzyme 5, peroxisomal OS=Cucurbita moschata OX=3662 GN=LOC111445243 PE=4 SV=1)

HSP 1 Score: 946.0 bits (2444), Expect = 6.9e-272
Identity = 453/557 (81.33%), Postives = 501/557 (89.95%), Query Frame = 0

Query: 1   MEDLKPRPANSFPLTPIGFIERAAIIYGDSTSIIYNSTTYTWSETHRRCLRLASSISSFG 60
           MEDLKP PANS PLTPIGF+ERAA++YGD  SIIYNSTTYTW+ETHRRCL+LASS+ S G
Sbjct: 1   MEDLKPNPANSSPLTPIGFLERAAVVYGDCPSIIYNSTTYTWTETHRRCLQLASSLVSIG 60

Query: 61  IQRGHVVSVIAPNTPAMYELHFAVPMVGAILNNINLRLDTRTISILLRHSESKLIFVDQA 120
           IQRG VVSV++PNT AMYELHFAVPM GA+LN INLRLD RTIS++LRH E+ LIFVDQA
Sbjct: 61  IQRGQVVSVVSPNTAAMYELHFAVPMAGAVLNTINLRLDARTISVILRHCETNLIFVDQA 120

Query: 121 SCAVIFEALALFPPEMKRPLLVLIADDTVEQDSTLPSFVSIRDNFVDSYEDMVKRGDSKF 180
           SC +I +ALALFPPEMKRP L+LIAD+   +DS+  S V  R +FVDSYE+MV++GD  F
Sbjct: 121 SCGLISDALALFPPEMKRPPLILIADNAAAEDSSSASSVWNRADFVDSYEEMVQKGDPLF 180

Query: 181 QWMRPLSEWDPIVLNYTSGTTSSPKGVVHCHRGIFTVTLDSLLEWGVPKQSVYLWTLPMF 240
           QW+RP SEWDPIVLNYTSGTTSSPKGVVHCHRGIFTVTLDSL+EWGVPK+SVYLWTLPMF
Sbjct: 181 QWIRPASEWDPIVLNYTSGTTSSPKGVVHCHRGIFTVTLDSLIEWGVPKESVYLWTLPMF 240

Query: 241 HGNGWSFPWGMAAVGGTNVCIRKFDATLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNLPL 300
           H NGWSFPWG+AAVGGTNVCIRKFDA  IF+LI R+HVTHLCGAPVVLNMLTNSPDN PL
Sbjct: 241 HANGWSFPWGIAAVGGTNVCIRKFDAAAIFALIHRYHVTHLCGAPVVLNMLTNSPDNRPL 300

Query: 301 DRPVKILTAGAPPPASILLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAIEQAR 360
           DRPVKILTAGAPPPA++L RTESLGF V+HGYGLTE+ G +ICCSWKS WN+LPA E+AR
Sbjct: 301 DRPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETGGLVICCSWKSGWNRLPAAERAR 360

Query: 361 LKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRGGSVMLGYLKDPEGTTKAMSD 420
           LKARQGVR LA+   DVLDPET KSVKRDG SIGEIV+RG S+MLGYLKDPE T KAM+ 
Sbjct: 361 LKARQGVRTLAMAVADVLDPETGKSVKRDGTSIGEIVLRGSSMMLGYLKDPEATAKAMTA 420

Query: 421 DGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPMVNEAAVVARPD 480
           +GWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNP VNEAAVVARPD
Sbjct: 421 EGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPD 480

Query: 481 EFWGETPCAFISLKEELTRKPTEAEIIEYCKGKLPRFMVPKTVIFMTEMPKTATGKIQKF 540
           EFWGETPCAF+SLKE LTR+PTE EIIEYC+GKLPRFMVPKTV+F  E+PKT+TGKIQKF
Sbjct: 481 EFWGETPCAFVSLKEGLTRRPTEGEIIEYCRGKLPRFMVPKTVVFTAELPKTSTGKIQKF 540

Query: 541 VLRQMAKSLSPAQKSRL 558
           VLR+MAKS+ PA KSRL
Sbjct: 541 VLREMAKSMGPAGKSRL 557

BLAST of PI0018013 vs. ExPASy TrEMBL
Match: A0A6J1JTQ2 (probable acyl-activating enzyme 5, peroxisomal OS=Cucurbita maxima OX=3661 GN=LOC111489657 PE=4 SV=1)

HSP 1 Score: 943.0 bits (2436), Expect = 5.8e-271
Identity = 454/561 (80.93%), Postives = 504/561 (89.84%), Query Frame = 0

Query: 1   MEDLKPRPANSFPLTPIGFIERAAIIYGDSTSIIYNSTTYTWSETHRRCLRLASSISSFG 60
           MEDLKP PANS PLTPIGF+ERAA++YGD  SIIYNSTTYTW+ETHRRCL+LASS+ S G
Sbjct: 1   MEDLKPNPANSSPLTPIGFLERAAVVYGDCPSIIYNSTTYTWTETHRRCLQLASSLVSIG 60

Query: 61  IQRGHVVSVIAPNTPAMYELHFAVPMVGAILNNINLRLDTRTISILLRHSESKLIFVDQA 120
           IQRG VVSV++PNTPAMYELHFAVPM GA+LN INLRLD+RTIS++LRH E+ LIFVDQA
Sbjct: 61  IQRGQVVSVVSPNTPAMYELHFAVPMAGAVLNTINLRLDSRTISVILRHCETNLIFVDQA 120

Query: 121 SCAVIFEALALFPPEMKRPLLVLIADDTVEQD----STLPSFVSIRDNFVDSYEDMVKRG 180
           SC +I +ALALFPPEMKRP L+LIAD+   +D    S+  S VS R +FVDSYE+MV++G
Sbjct: 121 SCGLISDALALFPPEMKRPPLILIADNAAAEDSSSSSSSASSVSNRADFVDSYEEMVQKG 180

Query: 181 DSKFQWMRPLSEWDPIVLNYTSGTTSSPKGVVHCHRGIFTVTLDSLLEWGVPKQSVYLWT 240
           D  FQW+RP+SEWDPIVLNYTSGTTSSPKGVVHCHRGIFTVTLDSL+EWGV KQSVYLWT
Sbjct: 181 DPLFQWIRPVSEWDPIVLNYTSGTTSSPKGVVHCHRGIFTVTLDSLIEWGVSKQSVYLWT 240

Query: 241 LPMFHGNGWSFPWGMAAVGGTNVCIRKFDATLIFSLIRRHHVTHLCGAPVVLNMLTNSPD 300
           LPMFH NGWSFPWG+AAVGGTNVCIRKFDA  IF+LI R+HVTHLCGAPVVLNMLTNSPD
Sbjct: 241 LPMFHANGWSFPWGIAAVGGTNVCIRKFDAATIFALIHRYHVTHLCGAPVVLNMLTNSPD 300

Query: 301 NLPLDRPVKILTAGAPPPASILLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAI 360
           N PLDRPVKILTAGAPPPA++L RTESLGF V+HGYGLTE+ G +ICCSWKS WN+LPA 
Sbjct: 301 NRPLDRPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETGGLVICCSWKSGWNRLPAA 360

Query: 361 EQARLKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRGGSVMLGYLKDPEGTTK 420
           E+ARLKARQGVR LA+   DVL PET KSVKRDG SIGEIV+RG S+MLGYLKDPE T K
Sbjct: 361 ERARLKARQGVRTLAMAVADVLCPETGKSVKRDGTSIGEIVLRGSSMMLGYLKDPEATAK 420

Query: 421 AMSDDGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPMVNEAAVV 480
           AM+ +GWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNP VNEAAVV
Sbjct: 421 AMTAEGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVV 480

Query: 481 ARPDEFWGETPCAFISLKEELTRKPTEAEIIEYCKGKLPRFMVPKTVIFMTEMPKTATGK 540
           ARPDEFWGETPCAF+SLKE LTR+PTE EIIEYC+GKLPRFMVPKTV+FM E+PKT+TGK
Sbjct: 481 ARPDEFWGETPCAFVSLKEGLTRRPTEGEIIEYCRGKLPRFMVPKTVVFMAELPKTSTGK 540

Query: 541 IQKFVLRQMAKSLSPAQKSRL 558
           IQKFVLR+MAKS+ PA +SRL
Sbjct: 541 IQKFVLREMAKSMGPAGRSRL 561

BLAST of PI0018013 vs. NCBI nr
Match: XP_008463174.1 (PREDICTED: probable acyl-activating enzyme 5, peroxisomal [Cucumis melo] >KAA0046220.1 putative acyl-activating enzyme 5, peroxisomal [Cucumis melo var. makuwa] >TYK18976.1 putative acyl-activating enzyme 5, peroxisomal [Cucumis melo var. makuwa])

HSP 1 Score: 1078.9 bits (2789), Expect = 0.0e+00
Identity = 533/557 (95.69%), Postives = 541/557 (97.13%), Query Frame = 0

Query: 1   MEDLKPRPANSFPLTPIGFIERAAIIYGDSTSIIYNSTTYTWSETHRRCLRLASSISSFG 60
           MEDLKP PANSFPLTPIGFI+RAA IYGDSTSIIYNSTTYTWSETHRRCL+LASSISSFG
Sbjct: 1   MEDLKPTPANSFPLTPIGFIQRAATIYGDSTSIIYNSTTYTWSETHRRCLQLASSISSFG 60

Query: 61  IQRGHVVSVIAPNTPAMYELHFAVPMVGAILNNINLRLDTRTISILLRHSESKLIFVDQA 120
           IQRGHVVSVIAPNTPAMYELHFAVPMVGAILN INLRLDTRTISILLRHSESKLIFVDQA
Sbjct: 61  IQRGHVVSVIAPNTPAMYELHFAVPMVGAILNTINLRLDTRTISILLRHSESKLIFVDQA 120

Query: 121 SCAVIFEALALFPPEMKRPLLVLIADDTVEQDSTLPSFVSIRDNFVDSYEDMVKRGDSKF 180
           SCA+IFEALALFPPEMKRPLLVLIADD  EQDS+LPSFVSIRDNFVDSYEDMVKRGDSKF
Sbjct: 121 SCALIFEALALFPPEMKRPLLVLIADDAAEQDSSLPSFVSIRDNFVDSYEDMVKRGDSKF 180

Query: 181 QWMRPLSEWDPIVLNYTSGTTSSPKGVVHCHRGIFTVTLDSLLEWGVPKQSVYLWTLPMF 240
           QW+RPLSEWDPIVLNYTSGTTSSPKGVVHCHRGIFTV LDSLLEWGVPKQSVYLWTLPMF
Sbjct: 181 QWIRPLSEWDPIVLNYTSGTTSSPKGVVHCHRGIFTVALDSLLEWGVPKQSVYLWTLPMF 240

Query: 241 HGNGWSFPWGMAAVGGTNVCIRKFDATLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNLPL 300
           HGNGWSFPWG+AAVGGTNVCIRKFDA LIFSLIRRHHVTHLCGAPVVLNMLTNSPDNLPL
Sbjct: 241 HGNGWSFPWGIAAVGGTNVCIRKFDARLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNLPL 300

Query: 301 DRPVKILTAGAPPPASILLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAIEQAR 360
           DRPVKILTAGAPPPASILLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAIEQAR
Sbjct: 301 DRPVKILTAGAPPPASILLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAIEQAR 360

Query: 361 LKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRGGSVMLGYLKDPEGTTKAMSD 420
           LKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRG SV LGYLKDPEGT KAMS+
Sbjct: 361 LKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRGASVTLGYLKDPEGTAKAMSE 420

Query: 421 DGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPMVNEAAVVARPD 480
           DGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLY NPMVNEAAVVARPD
Sbjct: 421 DGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYMNPMVNEAAVVARPD 480

Query: 481 EFWGETPCAFISLKEELTRKPTEAEIIEYCKGKLPRFMVPKTVIFMTEMPKTATGKIQKF 540
           EFWGETPCAFISLKE L+RKPTE EIIEYCKGKLPRFMVPKTVIFMTEMPKTATGKIQKF
Sbjct: 481 EFWGETPCAFISLKEGLSRKPTEEEIIEYCKGKLPRFMVPKTVIFMTEMPKTATGKIQKF 540

Query: 541 VLRQMAKSLSPAQKSRL 558
           VLRQ AKSL PA KSRL
Sbjct: 541 VLRQKAKSLGPAPKSRL 557

BLAST of PI0018013 vs. NCBI nr
Match: XP_004148908.1 (probable acyl-activating enzyme 5, peroxisomal [Cucumis sativus] >KGN50898.1 hypothetical protein Csa_023091 [Cucumis sativus])

HSP 1 Score: 1056.2 bits (2730), Expect = 9.7e-305
Identity = 517/557 (92.82%), Postives = 537/557 (96.41%), Query Frame = 0

Query: 1   MEDLKPRPANSFPLTPIGFIERAAIIYGDSTSIIYNSTTYTWSETHRRCLRLASSISSFG 60
           MEDLKPRPANSFPLTPIGFIERAAIIYGDSTSIIYNSTTYTWSETHRRCL+LASSISSFG
Sbjct: 1   MEDLKPRPANSFPLTPIGFIERAAIIYGDSTSIIYNSTTYTWSETHRRCLQLASSISSFG 60

Query: 61  IQRGHVVSVIAPNTPAMYELHFAVPMVGAILNNINLRLDTRTISILLRHSESKLIFVDQA 120
           IQRGHVVSV APNTPAMYELHFAVPM GAILN INLRLDTRTISILLRHSESKLIFVDQ 
Sbjct: 61  IQRGHVVSVFAPNTPAMYELHFAVPMAGAILNTINLRLDTRTISILLRHSESKLIFVDQP 120

Query: 121 SCAVIFEALALFPPEMKRPLLVLIADDTVEQDSTLPSFVSIRDNFVDSYEDMVKRGDSKF 180
           SCA+IF+ALALFPP+MKRPLLVLI DDT EQDS+LPS VSIRDNFVDSYE+MVK GDSKF
Sbjct: 121 SCALIFDALALFPPDMKRPLLVLITDDTAEQDSSLPSVVSIRDNFVDSYENMVKSGDSKF 180

Query: 181 QWMRPLSEWDPIVLNYTSGTTSSPKGVVHCHRGIFTVTLDSLLEWGVPKQSVYLWTLPMF 240
           QW+RPLSEWDPIVLNYTSGTTS+PKGVV+CHRG+FTV LDS+LEWGVPKQSVYLWTLPMF
Sbjct: 181 QWIRPLSEWDPIVLNYTSGTTSAPKGVVNCHRGVFTVALDSILEWGVPKQSVYLWTLPMF 240

Query: 241 HGNGWSFPWGMAAVGGTNVCIRKFDATLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNLPL 300
           HGNGWSFPWG+AAVGGTNVCIRKFD+ LIFSLIRRHHVTHLCGAPVVLNMLTNSPDNLPL
Sbjct: 241 HGNGWSFPWGIAAVGGTNVCIRKFDSRLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNLPL 300

Query: 301 DRPVKILTAGAPPPASILLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAIEQAR 360
           DRPVKILTAGAPPPAS+LLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAIEQAR
Sbjct: 301 DRPVKILTAGAPPPASVLLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAIEQAR 360

Query: 361 LKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRGGSVMLGYLKDPEGTTKAMSD 420
           LKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRG S+MLGYLKD E T+KAMS+
Sbjct: 361 LKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRGPSLMLGYLKDTEATSKAMSE 420

Query: 421 DGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPMVNEAAVVARPD 480
           DGWFFTGDVGVMHPDGY+EIKDRSKDVIISGGENLSSVEVESVLY NPMVNEAAVVARPD
Sbjct: 421 DGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPMVNEAAVVARPD 480

Query: 481 EFWGETPCAFISLKEELTRKPTEAEIIEYCKGKLPRFMVPKTVIFMTEMPKTATGKIQKF 540
           EFWGETPCAFISLKE L+RKPTE EIIEYCKGKLP+FMVPKTVIFMTEMPKTATGK QKF
Sbjct: 481 EFWGETPCAFISLKEGLSRKPTEEEIIEYCKGKLPKFMVPKTVIFMTEMPKTATGKFQKF 540

Query: 541 VLRQMAKSLSPAQKSRL 558
           VLRQ AKSL P QKSRL
Sbjct: 541 VLRQKAKSLGPPQKSRL 557

BLAST of PI0018013 vs. NCBI nr
Match: XP_022939278.1 (probable acyl-activating enzyme 5, peroxisomal [Cucurbita moschata] >KAG6579057.1 putative acyl-activating enzyme 5, peroxisomal, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 946.0 bits (2444), Expect = 1.4e-271
Identity = 453/557 (81.33%), Postives = 501/557 (89.95%), Query Frame = 0

Query: 1   MEDLKPRPANSFPLTPIGFIERAAIIYGDSTSIIYNSTTYTWSETHRRCLRLASSISSFG 60
           MEDLKP PANS PLTPIGF+ERAA++YGD  SIIYNSTTYTW+ETHRRCL+LASS+ S G
Sbjct: 1   MEDLKPNPANSSPLTPIGFLERAAVVYGDCPSIIYNSTTYTWTETHRRCLQLASSLVSIG 60

Query: 61  IQRGHVVSVIAPNTPAMYELHFAVPMVGAILNNINLRLDTRTISILLRHSESKLIFVDQA 120
           IQRG VVSV++PNT AMYELHFAVPM GA+LN INLRLD RTIS++LRH E+ LIFVDQA
Sbjct: 61  IQRGQVVSVVSPNTAAMYELHFAVPMAGAVLNTINLRLDARTISVILRHCETNLIFVDQA 120

Query: 121 SCAVIFEALALFPPEMKRPLLVLIADDTVEQDSTLPSFVSIRDNFVDSYEDMVKRGDSKF 180
           SC +I +ALALFPPEMKRP L+LIAD+   +DS+  S V  R +FVDSYE+MV++GD  F
Sbjct: 121 SCGLISDALALFPPEMKRPPLILIADNAAAEDSSSASSVWNRADFVDSYEEMVQKGDPLF 180

Query: 181 QWMRPLSEWDPIVLNYTSGTTSSPKGVVHCHRGIFTVTLDSLLEWGVPKQSVYLWTLPMF 240
           QW+RP SEWDPIVLNYTSGTTSSPKGVVHCHRGIFTVTLDSL+EWGVPK+SVYLWTLPMF
Sbjct: 181 QWIRPASEWDPIVLNYTSGTTSSPKGVVHCHRGIFTVTLDSLIEWGVPKESVYLWTLPMF 240

Query: 241 HGNGWSFPWGMAAVGGTNVCIRKFDATLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNLPL 300
           H NGWSFPWG+AAVGGTNVCIRKFDA  IF+LI R+HVTHLCGAPVVLNMLTNSPDN PL
Sbjct: 241 HANGWSFPWGIAAVGGTNVCIRKFDAAAIFALIHRYHVTHLCGAPVVLNMLTNSPDNRPL 300

Query: 301 DRPVKILTAGAPPPASILLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAIEQAR 360
           DRPVKILTAGAPPPA++L RTESLGF V+HGYGLTE+ G +ICCSWKS WN+LPA E+AR
Sbjct: 301 DRPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETGGLVICCSWKSGWNRLPAAERAR 360

Query: 361 LKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRGGSVMLGYLKDPEGTTKAMSD 420
           LKARQGVR LA+   DVLDPET KSVKRDG SIGEIV+RG S+MLGYLKDPE T KAM+ 
Sbjct: 361 LKARQGVRTLAMAVADVLDPETGKSVKRDGTSIGEIVLRGSSMMLGYLKDPEATAKAMTA 420

Query: 421 DGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPMVNEAAVVARPD 480
           +GWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNP VNEAAVVARPD
Sbjct: 421 EGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPD 480

Query: 481 EFWGETPCAFISLKEELTRKPTEAEIIEYCKGKLPRFMVPKTVIFMTEMPKTATGKIQKF 540
           EFWGETPCAF+SLKE LTR+PTE EIIEYC+GKLPRFMVPKTV+F  E+PKT+TGKIQKF
Sbjct: 481 EFWGETPCAFVSLKEGLTRRPTEGEIIEYCRGKLPRFMVPKTVVFTAELPKTSTGKIQKF 540

Query: 541 VLRQMAKSLSPAQKSRL 558
           VLR+MAKS+ PA KSRL
Sbjct: 541 VLREMAKSMGPAGKSRL 557

BLAST of PI0018013 vs. NCBI nr
Match: XP_022993747.1 (probable acyl-activating enzyme 5, peroxisomal [Cucurbita maxima])

HSP 1 Score: 943.0 bits (2436), Expect = 1.2e-270
Identity = 454/561 (80.93%), Postives = 504/561 (89.84%), Query Frame = 0

Query: 1   MEDLKPRPANSFPLTPIGFIERAAIIYGDSTSIIYNSTTYTWSETHRRCLRLASSISSFG 60
           MEDLKP PANS PLTPIGF+ERAA++YGD  SIIYNSTTYTW+ETHRRCL+LASS+ S G
Sbjct: 1   MEDLKPNPANSSPLTPIGFLERAAVVYGDCPSIIYNSTTYTWTETHRRCLQLASSLVSIG 60

Query: 61  IQRGHVVSVIAPNTPAMYELHFAVPMVGAILNNINLRLDTRTISILLRHSESKLIFVDQA 120
           IQRG VVSV++PNTPAMYELHFAVPM GA+LN INLRLD+RTIS++LRH E+ LIFVDQA
Sbjct: 61  IQRGQVVSVVSPNTPAMYELHFAVPMAGAVLNTINLRLDSRTISVILRHCETNLIFVDQA 120

Query: 121 SCAVIFEALALFPPEMKRPLLVLIADDTVEQD----STLPSFVSIRDNFVDSYEDMVKRG 180
           SC +I +ALALFPPEMKRP L+LIAD+   +D    S+  S VS R +FVDSYE+MV++G
Sbjct: 121 SCGLISDALALFPPEMKRPPLILIADNAAAEDSSSSSSSASSVSNRADFVDSYEEMVQKG 180

Query: 181 DSKFQWMRPLSEWDPIVLNYTSGTTSSPKGVVHCHRGIFTVTLDSLLEWGVPKQSVYLWT 240
           D  FQW+RP+SEWDPIVLNYTSGTTSSPKGVVHCHRGIFTVTLDSL+EWGV KQSVYLWT
Sbjct: 181 DPLFQWIRPVSEWDPIVLNYTSGTTSSPKGVVHCHRGIFTVTLDSLIEWGVSKQSVYLWT 240

Query: 241 LPMFHGNGWSFPWGMAAVGGTNVCIRKFDATLIFSLIRRHHVTHLCGAPVVLNMLTNSPD 300
           LPMFH NGWSFPWG+AAVGGTNVCIRKFDA  IF+LI R+HVTHLCGAPVVLNMLTNSPD
Sbjct: 241 LPMFHANGWSFPWGIAAVGGTNVCIRKFDAATIFALIHRYHVTHLCGAPVVLNMLTNSPD 300

Query: 301 NLPLDRPVKILTAGAPPPASILLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAI 360
           N PLDRPVKILTAGAPPPA++L RTESLGF V+HGYGLTE+ G +ICCSWKS WN+LPA 
Sbjct: 301 NRPLDRPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETGGLVICCSWKSGWNRLPAA 360

Query: 361 EQARLKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRGGSVMLGYLKDPEGTTK 420
           E+ARLKARQGVR LA+   DVL PET KSVKRDG SIGEIV+RG S+MLGYLKDPE T K
Sbjct: 361 ERARLKARQGVRTLAMAVADVLCPETGKSVKRDGTSIGEIVLRGSSMMLGYLKDPEATAK 420

Query: 421 AMSDDGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPMVNEAAVV 480
           AM+ +GWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNP VNEAAVV
Sbjct: 421 AMTAEGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVV 480

Query: 481 ARPDEFWGETPCAFISLKEELTRKPTEAEIIEYCKGKLPRFMVPKTVIFMTEMPKTATGK 540
           ARPDEFWGETPCAF+SLKE LTR+PTE EIIEYC+GKLPRFMVPKTV+FM E+PKT+TGK
Sbjct: 481 ARPDEFWGETPCAFVSLKEGLTRRPTEGEIIEYCRGKLPRFMVPKTVVFMAELPKTSTGK 540

Query: 541 IQKFVLRQMAKSLSPAQKSRL 558
           IQKFVLR+MAKS+ PA +SRL
Sbjct: 541 IQKFVLREMAKSMGPAGRSRL 561

BLAST of PI0018013 vs. NCBI nr
Match: XP_023550909.1 (probable acyl-activating enzyme 5, peroxisomal [Cucurbita pepo subsp. pepo])

HSP 1 Score: 937.6 bits (2422), Expect = 5.1e-269
Identity = 451/561 (80.39%), Postives = 501/561 (89.30%), Query Frame = 0

Query: 1   MEDLKPRPANSFPLTPIGFIERAAIIYGDSTSIIYNSTTYTWSETHRRCLRLASSISSFG 60
           MEDLKP PANS PLTPIGF+ERAA++YGD  SIIY+STTYTW+ETHRRCL+LASS+ S G
Sbjct: 1   MEDLKPNPANSSPLTPIGFLERAAVVYGDCRSIIYDSTTYTWTETHRRCLQLASSLVSIG 60

Query: 61  IQRGHVVSVIAPNTPAMYELHFAVPMVGAILNNINLRLDTRTISILLRHSESKLIFVDQA 120
           IQRG VVSV++PNT AMYELHFAVPM GA+LN INLRLD RTIS++LRH E+ LIFVDQA
Sbjct: 61  IQRGQVVSVVSPNTAAMYELHFAVPMAGAVLNTINLRLDARTISVILRHCETNLIFVDQA 120

Query: 121 SCAVIFEALALFPPEMKRPLLVLIADDTVEQD----STLPSFVSIRDNFVDSYEDMVKRG 180
           SC +I +ALALFPPEMKRP L+LIAD+   +D    S+  S V  R +FVDSYE+MV++G
Sbjct: 121 SCGLISDALALFPPEMKRPPLILIADNAAAEDSSSSSSSASSVLNRADFVDSYEEMVQKG 180

Query: 181 DSKFQWMRPLSEWDPIVLNYTSGTTSSPKGVVHCHRGIFTVTLDSLLEWGVPKQSVYLWT 240
           D  FQW+RP SEWDPIVLNYTSGTTSSPKGVVHCHRGIFTVTLDSL+EWGVPK+SVYLWT
Sbjct: 181 DPLFQWIRPASEWDPIVLNYTSGTTSSPKGVVHCHRGIFTVTLDSLIEWGVPKESVYLWT 240

Query: 241 LPMFHGNGWSFPWGMAAVGGTNVCIRKFDATLIFSLIRRHHVTHLCGAPVVLNMLTNSPD 300
           LPMFH NGWSFPWG+AAVGGTNVCIRKFDA  IF+LI R+HVTHLCGAPVVLNMLTNSPD
Sbjct: 241 LPMFHANGWSFPWGIAAVGGTNVCIRKFDAAAIFALIHRYHVTHLCGAPVVLNMLTNSPD 300

Query: 301 NLPLDRPVKILTAGAPPPASILLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAI 360
           N PLDRPVKILTAGAPPPA++L RTESLGF V+HGYGLTE+ G +ICCSWKS WN+LPA 
Sbjct: 301 NRPLDRPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETGGLVICCSWKSGWNRLPAA 360

Query: 361 EQARLKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRGGSVMLGYLKDPEGTTK 420
           ++ARLKARQGVR LA+   DVLDPET KSVKRDG SIGEIV+RG S+MLGYLKDPE T K
Sbjct: 361 DRARLKARQGVRTLAMAVADVLDPETGKSVKRDGTSIGEIVLRGSSMMLGYLKDPEATAK 420

Query: 421 AMSDDGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPMVNEAAVV 480
           AM+ +GWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNP VNEAAVV
Sbjct: 421 AMTAEGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVV 480

Query: 481 ARPDEFWGETPCAFISLKEELTRKPTEAEIIEYCKGKLPRFMVPKTVIFMTEMPKTATGK 540
           ARPDEFWGETPCAF+SLKE LTR+PTE EIIEYC+GKLPRFMVPKTV+F  E+PKT+TGK
Sbjct: 481 ARPDEFWGETPCAFVSLKEGLTRRPTEGEIIEYCRGKLPRFMVPKTVVFTAELPKTSTGK 540

Query: 541 IQKFVLRQMAKSLSPAQKSRL 558
           IQKFVLR+MAKS+ PA KSRL
Sbjct: 541 IQKFVLREMAKSMGPAGKSRL 561

BLAST of PI0018013 vs. TAIR 10
Match: AT5G16370.1 (acyl activating enzyme 5 )

HSP 1 Score: 765.4 bits (1975), Expect = 3.2e-221
Identity = 365/558 (65.41%), Postives = 459/558 (82.26%), Query Frame = 0

Query: 1   MEDLKPRPANSFPLTPIGFIERAAIIYGDSTSIIYNS-TTYTWSETHRRCLRLASSISSF 60
           ME +KP  ANS PLTPIGF+ERAA +YGD TSI+Y S T YTW ET+ RCLR+ASS+SS 
Sbjct: 1   MEQMKPCAANSPPLTPIGFLERAATVYGDCTSIVYGSNTVYTWRETNLRCLRVASSLSSI 60

Query: 61  GIQRGHVVSVIAPNTPAMYELHFAVPMVGAILNNINLRLDTRTISILLRHSESKLIFVDQ 120
           GI R  VVSV++PNTPAMYEL FAVPM GAILNNIN RLD RT+S+LLRH  SKL+FVD 
Sbjct: 61  GIGRSDVVSVLSPNTPAMYELQFAVPMSGAILNNINTRLDARTVSVLLRHCGSKLLFVDV 120

Query: 121 ASCAVIFEALALFPPEMKRPLLVLIADDTVEQDSTLPSFVSIRDNFVDSYEDMVKRGDSK 180
            S  +  EA+++   +   P+LV IAD   +++    + V+ R  F  +Y+D++ RGD  
Sbjct: 121 FSVDLAVEAISMMTTD--PPILVFIAD---KEEEGGDADVADRTKFSYTYDDLIHRGDLD 180

Query: 181 FQWMRPLSEWDPIVLNYTSGTTSSPKGVVHCHRGIFTVTLDSLLEWGVPKQSVYLWTLPM 240
           F+W+RP SEWDP+VLNYTSGTTS+PKGVVHCHRGIF +++DSL++W VPK  VYLWTLP+
Sbjct: 181 FKWIRPESEWDPVVLNYTSGTTSAPKGVVHCHRGIFVMSIDSLIDWTVPKNPVYLWTLPI 240

Query: 241 FHGNGWSFPWGMAAVGGTNVCIRKFDATLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNLP 300
           FH NGWS+PWG+AAVGGTNVC+RKFDA LI+ LIR H VTH+CGAPVVLNML+ + +  P
Sbjct: 241 FHANGWSYPWGIAAVGGTNVCLRKFDAPLIYRLIRDHGVTHMCGAPVVLNMLSATNEFQP 300

Query: 301 LDRPVKILTAGAPPPASILLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAIEQA 360
           L+RPV ILTAGAPPPA++LLR ES+GFV++HGYGLTE+AG  + C+WK +WN+LPA ++A
Sbjct: 301 LNRPVNILTAGAPPPAAVLLRAESIGFVISHGYGLTETAGLNVSCAWKPQWNRLPASDRA 360

Query: 361 RLKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRGGSVMLGYLKDPEGTTKAMS 420
           RLKARQGVR +    +DV+DPE+ +SV+R+G ++GEIV+RG S+MLGYLKDP GT KA+ 
Sbjct: 361 RLKARQGVRTVGFTEIDVVDPESGRSVERNGETVGEIVMRGSSIMLGYLKDPVGTEKALK 420

Query: 421 DDGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPMVNEAAVVARP 480
            +GWF+TGDVGV+H DGYLEIKDRSKD+II+GGEN+SSVEVE+VLYTNP VNE AVVARP
Sbjct: 421 -NGWFYTGDVGVIHSDGYLEIKDRSKDIIITGGENVSSVEVETVLYTNPAVNEVAVVARP 480

Query: 481 DEFWGETPCAFISLKEELTRKPTEAEIIEYCKGKLPRFMVPKTVIFMTEMPKTATGKIQK 540
           D FWGETPCAF+SLK  LT++PTE E+IEYC+ K+P++MVPKTV F+ E+PKT+TGK+ K
Sbjct: 481 DVFWGETPCAFVSLKSGLTQRPTEVEMIEYCRKKMPKYMVPKTVSFVDELPKTSTGKVMK 540

Query: 541 FVLRQMAKSLSPAQKSRL 558
           FVLR++AK +   + SR+
Sbjct: 541 FVLREIAKKMGTTRLSRM 552

BLAST of PI0018013 vs. TAIR 10
Match: AT5G16340.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 755.0 bits (1948), Expect = 4.3e-218
Identity = 360/550 (65.45%), Postives = 452/550 (82.18%), Query Frame = 0

Query: 1   MEDLKPRPANSFPLTPIGFIERAAIIYGDSTSIIYNS-TTYTWSETHRRCLRLASSISSF 60
           ME++KP  ANS PLTPIGF+ERAA +YGD TSI+Y S T YTW ET+ RCLR+ASS+SS 
Sbjct: 1   MEEMKPCAANSPPLTPIGFLERAATVYGDCTSIVYGSNTVYTWRETNLRCLRVASSLSSI 60

Query: 61  GIQRGHVVSVIAPNTPAMYELHFAVPMVGAILNNINLRLDTRTISILLRHSESKLIFVDQ 120
           GI R  VVSV++PNTPAMYEL FAVPM GAILNNIN RLD RT+S+LLRH ESKL+FVD 
Sbjct: 61  GIGRSDVVSVLSPNTPAMYELQFAVPMSGAILNNINTRLDARTVSVLLRHCESKLLFVDV 120

Query: 121 ASCAVIFEALALFPPEMKRPLLVLIADDTVEQDSTLPSFVSIRDNFVDSYEDMVKRGDSK 180
            S  +  EA+++   +   P+LV+IAD   E++  +     +   F  +Y+D+++RGD  
Sbjct: 121 FSVDLAVEAVSMMTTD--PPILVVIADK--EEEGGVADVADL-SKFSYTYDDLIERGDPG 180

Query: 181 FQWMRPLSEWDPIVLNYTSGTTSSPKGVVHCHRGIFTVTLDSLLEWGVPKQSVYLWTLPM 240
           F+W+RP SEWDP+VLNYTSGTTS+PKGVVHCHRGIF +++DSL++W VPK  VYLWTLP+
Sbjct: 181 FKWIRPESEWDPVVLNYTSGTTSAPKGVVHCHRGIFVMSVDSLIDWAVPKNPVYLWTLPI 240

Query: 241 FHGNGWSFPWGMAAVGGTNVCIRKFDATLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNLP 300
           FH NGW+ PWG+AAVGGTNVC+RKFDA LI+ LIR H VTH+CGAPVVLNML+ + ++ P
Sbjct: 241 FHSNGWTNPWGIAAVGGTNVCLRKFDAPLIYRLIRDHGVTHMCGAPVVLNMLSATQESQP 300

Query: 301 LDRPVKILTAGAPPPASILLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAIEQA 360
           L+ PV ILTAG+PPPA++LLR ES+GFV++HGYGLTE+AG I+ C+WK +WN LPA ++A
Sbjct: 301 LNHPVNILTAGSPPPATVLLRAESIGFVISHGYGLTETAGVIVSCAWKPKWNHLPASDRA 360

Query: 361 RLKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRGGSVMLGYLKDPEGTTKAMS 420
           RLKARQGVR +    +DV+DPE+  SV+R+G ++GEIV+RG SVMLGYLKDP GT KA+ 
Sbjct: 361 RLKARQGVRTVGFTEIDVVDPESGLSVERNGETVGEIVMRGSSVMLGYLKDPVGTEKALK 420

Query: 421 DDGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPMVNEAAVVARP 480
            +GWF+TGDVGV+H DGYLEIKDRSKD+II+GGEN+SSVEVE+VLYT P VNE AVVARP
Sbjct: 421 -NGWFYTGDVGVIHSDGYLEIKDRSKDIIITGGENVSSVEVETVLYTIPAVNEVAVVARP 480

Query: 481 DEFWGETPCAFISLKEELTRKPTEAEIIEYCKGKLPRFMVPKTVIFMTEMPKTATGKIQK 540
           DEFWGETPCAF+SLK   + KPTE E++EYC+ K+P++MVPKTV FM E+PK++TGK+ K
Sbjct: 481 DEFWGETPCAFVSLKNGFSGKPTEEELMEYCRKKMPKYMVPKTVSFMDELPKSSTGKVTK 540

Query: 541 FVLRQMAKSL 550
           FVLR +AK +
Sbjct: 541 FVLRDIAKKM 544

BLAST of PI0018013 vs. TAIR 10
Match: AT1G75960.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 730.3 bits (1884), Expect = 1.1e-210
Identity = 355/551 (64.43%), Postives = 441/551 (80.04%), Query Frame = 0

Query: 1   MEDLKPRPANSFPLTPIGFIERAAIIYGDSTSIIY-NSTTYTWSETHRRCLRLASSISSF 60
           MEDLKP  ANS PLT +GF+ERAA +YGD TSI+Y NST YTW ET+ RCL +AS++SS 
Sbjct: 1   MEDLKPSAANSLPLTLLGFLERAATVYGDCTSIVYGNSTVYTWRETNHRCLCVASALSSI 60

Query: 61  GIQRGHVVSVIAPNTPAMYELHFAVPMVGAILNNINLRLDTRTISILLRHSESKLIFVDQ 120
           GI R  VVSV++ NTP MYEL F+VPM GAILNNIN RLD RT+S+LLRH ESKL+FVD 
Sbjct: 61  GIGRSDVVSVLSANTPEMYELQFSVPMSGAILNNINTRLDARTVSVLLRHCESKLLFVDF 120

Query: 121 ASCAVIFEALALFPPEMKRPLLVLIADDTVEQDSTLPSFVSIRDNFVDSYEDMVKRGDSK 180
               +  EA+ +    +  P+LVLIA++  E+     + V+ R  F   Y D++ RG+  
Sbjct: 121 FYSDLAVEAITML---LNPPILVLIANEEEEEGG---AEVTERSKFCYLYSDLITRGNPD 180

Query: 181 FQWMRPLSEWDPIVLNYTSGTTSSPKGVVHCHRGIFTVTLDSLLEWGVPKQSVYLWTLPM 240
           F+W+RP SEWDPIV+NYTSGTTSSPKGVVHCHRGIF +TLDSL +W VPK  VYLWTLP+
Sbjct: 181 FKWIRPGSEWDPIVVNYTSGTTSSPKGVVHCHRGIFVMTLDSLTDWAVPKTPVYLWTLPI 240

Query: 241 FHGNGWSFPWGMAAVGGTNVCIRKFDATLIFSLIRRHHVTHLCGAPVVLNMLTNSPD-NL 300
           FH NGW++PWG+AAVGGTNVC+RK  A  I+ LIR H VTH+ GAP+VL +L+ S + + 
Sbjct: 241 FHANGWTYPWGIAAVGGTNVCVRKLHAPSIYHLIRDHGVTHMYGAPIVLQILSASQESDQ 300

Query: 301 PLDRPVKILTAGAPPPASILLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAIEQ 360
           PL  PV  LTAG+ PPA++LLR ESLGF+V+HGYGLTE+AG I+ C+WK  WN+LPA +Q
Sbjct: 301 PLKSPVNFLTAGSSPPATVLLRAESLGFIVSHGYGLTETAGVIVSCAWKPNWNRLPASDQ 360

Query: 361 ARLKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRGGSVMLGYLKDPEGTTKAM 420
           A+LK+RQGVR +    +DV+DPE+ +SV+RDG ++GEIV+RG S+MLGYLK+P GT  + 
Sbjct: 361 AQLKSRQGVRTVGFSEIDVVDPESGRSVERDGETVGEIVLRGSSIMLGYLKNPIGTQNSF 420

Query: 421 SDDGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPMVNEAAVVAR 480
             +GWFFTGD+GV+H DGYLEIKDRSKDVIISGGEN+SSVEVE+VLYTNP VNEAAVVAR
Sbjct: 421 K-NGWFFTGDLGVIHGDGYLEIKDRSKDVIISGGENVSSVEVEAVLYTNPAVNEAAVVAR 480

Query: 481 PDEFWGETPCAFISLKEELTRKPTEAEIIEYCKGKLPRFMVPKTVIFMTEMPKTATGKIQ 540
           PDEFWGETPCAF+SLK  LTRKPT+ EIIEYCK K+PR+M PKTV F+ E+PKT+TGKI 
Sbjct: 481 PDEFWGETPCAFVSLKPGLTRKPTDKEIIEYCKYKMPRYMAPKTVSFLEELPKTSTGKII 540

Query: 541 KFVLRQMAKSL 550
           K +L+++AK++
Sbjct: 541 KSLLKEIAKNM 544

BLAST of PI0018013 vs. TAIR 10
Match: AT1G21540.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 714.1 bits (1842), Expect = 8.5e-206
Identity = 345/554 (62.27%), Postives = 434/554 (78.34%), Query Frame = 0

Query: 1   MEDLKPRPANSFPLTPIGFIERAAIIYGDSTSIIY-NSTTYTWSETHRRCLRLASSI--S 60
           ME L P P+NS PLT +GF++RAA +YGD  SI++  +T +TWSETH RCLR+AS++  S
Sbjct: 1   MELLLPHPSNSTPLTVLGFLDRAASVYGDCPSILHTTNTVHTWSETHNRCLRIASALTSS 60

Query: 61  SFGIQRGHVVSVIAPNTPAMYELHFAVPMVGAILNNINLRLDTRTISILLRHSESKLIFV 120
           S GI RG VVSV+ PN P++YEL FAVPM GAILNNIN RLD   +S+LLRHSESKL+FV
Sbjct: 61  SLGINRGQVVSVVGPNVPSVYELQFAVPMSGAILNNINPRLDAHALSVLLRHSESKLVFV 120

Query: 121 DQASCAVIFEALALFPPEMKRPLLVLIADDTVEQDSTLPSFVSIRDNFVDSYEDMVKRGD 180
           D  S +V+ EA++ F  + ++P LVL+ DD  E  S  PS  S   +F+D+Y+ +++RGD
Sbjct: 121 DPNSISVVLEAVS-FMRQNEKPHLVLL-DDDQEDGSLSPSAAS---DFLDTYQGVMERGD 180

Query: 181 SKFQWMRPLSEWDPIVLNYTSGTTSSPKGVVHCHRGIFTVTLDSLLEWGVPKQSVYLWTL 240
           S+F+W+RP +EW P++LNYTSGTTSSPKGVV  HR IF +T+ SLL+W  P + VYLWTL
Sbjct: 181 SRFKWIRPQTEWQPMILNYTSGTTSSPKGVVLSHRAIFMLTVSSLLDWHFPNRPVYLWTL 240

Query: 241 PMFHGNGWSFPWGMAAVGGTNVCIRKFDATLIFSLIRRHHVTHLCGAPVVLNMLTNSPDN 300
           PMFH NGW + WG AAVG TNVC R+ DA  I+ LI +HHVTH+C AP+VLNMLTN P  
Sbjct: 241 PMFHANGWGYTWGTAAVGATNVCTRRVDAPTIYDLIDKHHVTHMCAAPMVLNMLTNYPSR 300

Query: 301 LPLDRPVKILTAGAPPPASILLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAIE 360
            PL  PV+++TAGAPPPA+I+ R E+LGF V HGYGLTE+ G ++ C+WK+EW+ L  +E
Sbjct: 301 KPLKNPVQVMTAGAPPPAAIISRAETLGFNVGHGYGLTETGGPVVSCAWKAEWDHLDPLE 360

Query: 361 QARLKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRGGSVMLGYLKDPEGTTKA 420
           +ARLK+RQGVR +    VDV DP T KSV+ DG S+GEIV++GGSVMLGY KDPEGT   
Sbjct: 361 RARLKSRQGVRTIGFAEVDVRDPRTGKSVEHDGVSVGEIVLKGGSVMLGYYKDPEGTAAC 420

Query: 421 MSDDGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPMVNEAAVVA 480
           M +DGWF++GDVGV+H DGYLE+KDRSKDVII GGEN+SS EVE+VLYTNP+V EAAVVA
Sbjct: 421 MREDGWFYSGDVGVIHEDGYLEVKDRSKDVIICGGENISSAEVETVLYTNPVVKEAAVVA 480

Query: 481 RPDEFWGETPCAFISLKEELTRK--PTEAEIIEYCKGKLPRFMVPKTVIFMTEMPKTATG 540
           +PD+ WGETPCAF+SLK +       TE EI E+CK +LP++MVP+ VIF  E+PKT+TG
Sbjct: 481 KPDKMWGETPCAFVSLKYDSNGNGLVTEREIREFCKTRLPKYMVPRKVIFQEELPKTSTG 540

Query: 541 KIQKFVLRQMAKSL 550
           KIQKF+LRQMAKSL
Sbjct: 541 KIQKFLLRQMAKSL 549

BLAST of PI0018013 vs. TAIR 10
Match: AT1G77240.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 703.4 bits (1814), Expect = 1.5e-202
Identity = 332/551 (60.25%), Postives = 430/551 (78.04%), Query Frame = 0

Query: 1   MEDLKPRPANSFPLTPIGFIERAAIIYGDSTSIIYNSTTYTWSETHRRCLRLASSIS--S 60
           ME L P  +NS PLT +GF+ERAA ++GDS S+++ +T +TWSETH RCLR+AS++S  S
Sbjct: 1   MELLLPHASNSCPLTVLGFLERAASVFGDSPSLLHTTTVHTWSETHSRCLRIASTLSSAS 60

Query: 61  FGIQRGHVVSVIAPNTPAMYELHFAVPMVGAILNNINLRLDTRTISILLRHSESKLIFVD 120
            GI RG VVSVI PN P++YEL FAVPM GA+LNNIN RLD   +S+LLRHSESKL+FVD
Sbjct: 61  LGINRGQVVSVIGPNVPSVYELQFAVPMSGAVLNNINPRLDAHALSVLLRHSESKLVFVD 120

Query: 121 QASCAVIFEALALFPPEMKRPLLVLIADDTVEQDSTLPSFVSIRDNFVDSYEDMVKRGDS 180
             S +++ EA++ F P+ +RP LV++ D      + +PS  S   +F+D+YE  ++RGD 
Sbjct: 121 HHSSSLVLEAVS-FLPKDERPRLVILND-----GNDMPSSSSADMDFLDTYEGFMERGDL 180

Query: 181 KFQWMRPLSEWDPIVLNYTSGTTSSPKGVVHCHRGIFTVTLDSLLEWGVPKQSVYLWTLP 240
           +F+W+RP SEW P+VLNYTSGTTSSPKGVVH HR +F  T++SLL+W +P + VYLWTLP
Sbjct: 181 RFKWVRPKSEWTPMVLNYTSGTTSSPKGVVHSHRSVFMSTINSLLDWSLPNRPVYLWTLP 240

Query: 241 MFHGNGWSFPWGMAAVGGTNVCIRKFDATLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNL 300
           MFH NGWS+ W  AAVG  N+C+ + D   IF+LI ++ VTH+C AP+VLNMLTN P   
Sbjct: 241 MFHANGWSYTWATAAVGARNICVTRVDVPTIFNLIDKYQVTHMCAAPMVLNMLTNHPAQK 300

Query: 301 PLDRPVKILTAGAPPPASILLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAIEQ 360
           PL  PVK++TAGAPPPA+++ + E+LGF V+HGYG+TE+ G ++ C+ K EW++L   E+
Sbjct: 301 PLQSPVKVMTAGAPPPATVISKAEALGFDVSHGYGMTETGGLVVSCALKPEWDRLEPDER 360

Query: 361 ARLKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRGGSVMLGYLKDPEGTTKAM 420
           A+ K+RQG+R      VDV DP + KSVK DGA++GEIV RGGSVMLGY KDPEGT  +M
Sbjct: 361 AKQKSRQGIRTAVFAEVDVRDPISGKSVKHDGATVGEIVFRGGSVMLGYYKDPEGTAASM 420

Query: 421 SDDGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPMVNEAAVVAR 480
            +DGWF+TGD+GVMHPDGYLE+KDRSKDV+I GGEN+SS E+E+VLYTNP + EAAVVA+
Sbjct: 421 REDGWFYTGDIGVMHPDGYLEVKDRSKDVVICGGENISSTELEAVLYTNPAIKEAAVVAK 480

Query: 481 PDEFWGETPCAFISLKEELTRKPTEAEIIEYCKGKLPRFMVPKTVIFMTEMPKTATGKIQ 540
           PD+ WGETPCAF+SLK       TE EI E+CK KLP++MVP+ V+F+ E+PKT+TGKIQ
Sbjct: 481 PDKMWGETPCAFVSLKYH-DGSVTEREIREFCKTKLPKYMVPRNVVFLEELPKTSTGKIQ 540

Query: 541 KFVLRQMAKSL 550
           KF+LRQMAKSL
Sbjct: 541 KFLLRQMAKSL 544