PI0015536 (gene) Melon (PI 482460) v1

Overview


Name	PI0015536
Type	gene
Organism	Cucumis metuliferus (Melon (PI 482460) v1)
Description	Prolyl-tRNA synthetase
Location	chr05: 6793484 .. 6795644 (-)
RNA-Seq Expression	PI0015536
Synteny	PI0015536

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCTTCTGTGCAGTGTGTACAATGTTTACAAGGTTGTGCAGGCAAGAAAAGGAAACATGCTATTAGCATTAGCGATGGGAAAATTGCATTGTCCATTGACTTTGGTTTGTCTATGTTATGATGTAATAATATAGATATTTTGTTTCATTCTTCATACAGCTTTACCCGTTTGTAGTGCTTGTCGGAGGAGTCTTAGCATGGTAAATTTCAATTGTATTTGCATTTCCCTCACCTAGGACATTTGTAGTCATCATATTTCTTTACACCATATATGTCTCTCATTTAGGGATCATTTGTCACCTTCTGACATTATTGGAAGCTATCCCCATTTGGTTATAACTGGAACTGGACTTGCTTTTGGATTCTTAGTGGTAAGACATAGGTTCCCTTTCTTGTCTCTCTTTCACCATCTTTCTCCCTATCTTTTGAAGCTATGACATGGATTTGGTGGACTGAGTTGGTTTTTTTTAGTGTTATTGCTATTAAATGGAGGTTTTGATGAAGTACCATGTCACCATTTTCTTGTTGTTCTGATCTGACAAATGTTAAAGAGTCAGCGTTATGCCCCATGATTGGGAACCATTGTACGGGATCGATGACATACATTAGTTGGAGTTGGGACCTTTAATACAATGAACTGTAGCATTTCTTGAAACTAAAATAGAAGCGTTTTATTTTGCAGGGTAGAATGATTCTAGCCCACCTTTGTGATGAACCCAAGGGCTTGAAAACTGGAATGTGCATGGTATGTTACTTTAATGAACCAAAATACCATAGACAAATTCATCATTATTTGCTGATGCTAATATATGACACCAAAAAGTTGTTGATAGTATTTAGTCAGAATATCACCTTTGCATCAAGTCAACTGACTTTTCAAGCCTTGGCTAGCTATGGGGTGCAGAATTGGCTGTTTTGAAAATCTCCCTGAAGAGGCACGATTATGACATTATATAAGCCTATCCCTTATGCTACACTGTAATTTGATCTATAGTTTAAAAATGAAATTATCTGGGCTTGAACTACCCTTAATAGAAATACTTAAACCTTAATGTTTACTTTGATTTTTTTAGTTGAATGAAATTATACATAGTTAAATTATTAAGTCACAGCTCATATACATAAAAAAGAAGCTATACTTTCTTTTTCTAGGTACGTGCAGTTCGGCGTGATAATTCTGCAAAGGACATACCTAGGGATTCGTTGGTTGAACAAGTGAAAGAGTTGCTAGAAAGTATTCAACAAAGCCTTTTTGATGCGGCAAAAGAAAAACAAGATGCATGCATTCAGGTTATTAATACATGGGAGGAGTTTACTGAAGCCCTTGGTCAGAAGAAAATGATATTAGCTCCATGGTGTGATGAAGAGGTACAGCTCCAGTGTTTATTGTCGCTGTTTTGAAATTGCACTAGTGTTGTTTATTACATATATTAAGGACCGCCTACTTTTTGGTTCTGACTTTTCACGAGGCCACCAAAATTTTATTTGTTAGTTGAAGGGATCCTGGTTACTATTCTTTTCCAGTAGTCAATACGCTTTCATATGCTGAAATCTATTTGATGAATTAACTATAAGATCCTGGTTTGTTGTTGAATTAGTTTTTTGTGGAGCTTGTTTTTGTAGCTAGGAAGGGATATTTTGTTGCCATCTTTCTGCAGCGTAAATAATATTTCATTAGGACGATTTCTATGGAATTGAGGGGATAATTATCAATAAAATTTTGAACTGCATTGATCACTGGCTTGAGTCGATGCATTTTTAAGTATTTAGTTTCTATCGAAGTCTGTGGTAGCTATGTGGATAAATGGAGATAAGTTTTCTGAAATCTGAAGTATTTAATATATTTGTTATATTCCAACCTCTATCATTTACTAGTGTATTTCTAAAGGATGATGGTTTCATTGTTATGTTCCTCCAGGAGGTCGAGAAGGATGTGAAAACAAGGACTAAAGGTGAGGTGGGAGCAGCGAAAACCTTATGTTCCCCGTTCGAGCAGCCTCCCCTTCCAGAAGGTTACTAAATCCATGTCCAAATCTGTATTCATAACTTGTGTGATTTTTTTAAAAATTAAATTGATGATGTATGTGGAAAGAGTCATGTATTGATCCTTCAAAAAAATTAATTGCATCTTTTCTTGTTTTAGTTGTTTGTTTTTCATAG

mRNA sequence

ATGCTTCTGTGCAGTGTGTACAATGTTTACAAGCTTTACCCGTTTGTAGTGCTTGTCGGAGGAGTCTTAGCATGGGATCATTTGTCACCTTCTGACATTATTGGAAGCTATCCCCATTTGGTTATAACTGGAACTGGACTTGCTTTTGGATTCTTAGTGGGTAGAATGATTCTAGCCCACCTTTGTGATGAACCCAAGGGCTTGAAAACTGGAATGTGCATGGTACGTGCAGTTCGGCGTGATAATTCTGCAAAGGACATACCTAGGGATTCGTTGGTTGAACAAGTGAAAGAGTTGCTAGAAAGTATTCAACAAAGCCTTTTTGATGCGGCAAAAGAAAAACAAGATGCATGCATTCAGGTTATTAATACATGGGAGGAGTTTACTGAAGCCCTTGGTCAGAAGAAAATGATATTAGCTCCATGGTGTGATGAAGAGGAGGTCGAGAAGGATGTGAAAACAAGGACTAAAGGTGAGGTGGGAGCAGCGAAAACCTTATGTTCCCCGTTCGAGCAGCCTCCCCTTCCAGAAGTTGTTTGTTTTTCATAG

Coding sequence (CDS)

ATGCTTCTGTGCAGTGTGTACAATGTTTACAAGCTTTACCCGTTTGTAGTGCTTGTCGGAGGAGTCTTAGCATGGGATCATTTGTCACCTTCTGACATTATTGGAAGCTATCCCCATTTGGTTATAACTGGAACTGGACTTGCTTTTGGATTCTTAGTGGGTAGAATGATTCTAGCCCACCTTTGTGATGAACCCAAGGGCTTGAAAACTGGAATGTGCATGGTACGTGCAGTTCGGCGTGATAATTCTGCAAAGGACATACCTAGGGATTCGTTGGTTGAACAAGTGAAAGAGTTGCTAGAAAGTATTCAACAAAGCCTTTTTGATGCGGCAAAAGAAAAACAAGATGCATGCATTCAGGTTATTAATACATGGGAGGAGTTTACTGAAGCCCTTGGTCAGAAGAAAATGATATTAGCTCCATGGTGTGATGAAGAGGAGGTCGAGAAGGATGTGAAAACAAGGACTAAAGGTGAGGTGGGAGCAGCGAAAACCTTATGTTCCCCGTTCGAGCAGCCTCCCCTTCCAGAAGTTGTTTGTTTTTCATAG

Protein sequence

MLLCSVYNVYKLYPFVVLVGGVLAWDHLSPSDIIGSYPHLVITGTGLAFGFLVGRMILAHLCDEPKGLKTGMCMVRAVRRDNSAKDIPRDSLVEQVKELLESIQQSLFDAAKEKQDACIQVINTWEEFTEALGQKKMILAPWCDEEEVEKDVKTRTKGEVGAAKTLCSPFEQPPLPEVVCFS

Homology

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BLAST of PI0015536 vs. ExPASy Swiss-Prot
Match: Q9M1R2 (Proline--tRNA ligase, cytoplasmic OS=Arabidopsis thaliana OX=3702 GN=At3g62120 PE=1 SV=1)

HSP 1 Score: 159.8 bits (403), Expect = 2.8e-38
Identity = 76/110 (69.09%), Postives = 93/110 (84.55%), Query Frame = 0


Query: 75  VRAVRRDNSAK-DIPRDSLVEQVKELLESIQQSLFDAAKEKQDACIQVINTWEEFTEALG 134
           VR VRRDN  K DIPR SLVE VKELLE IQQ++++ AK+K++AC+Q + TW+EF +AL 
Sbjct: 406 VRTVRRDNGVKEDIPRGSLVEHVKELLEKIQQNMYEVAKQKREACVQEVKTWDEFIKALN 465

Query: 135 QKKMILAPWCDEEEVEKDVKTRTKGEVGAAKTLCSPFEQPPLPE-VVCFS 183
           +KK+ILAPWCDEEEVE+DVK RTKGE GAAKTLCSPF+QP LPE  +CF+
Sbjct: 466 EKKLILAPWCDEEEVERDVKARTKGETGAAKTLCSPFDQPELPEGTLCFA 515

[Close]

BLAST of PI0015536 vs. ExPASy Swiss-Prot
Match: O82567 (Choline/ethanolaminephosphotransferase 1 OS=Arabidopsis thaliana OX=3702 GN=AAPT1 PE=1 SV=1)

HSP 1 Score: 117.1 bits (292), Expect = 2.1e-25
Identity = 52/63 (82.54%), Postives = 58/63 (92.06%), Query Frame = 0


Query: 12  LYPFVVLVGGVLAWDHLSPSDIIGSYPHLVITGTGLAFGFLVGRMILAHLCDEPKGLKTG 71
           LYPFVVL+GGVL WD+LSP ++I +YPHLV+ GTGLAFGFLVGRMILAHLCDEPKGLKT 
Sbjct: 259 LYPFVVLLGGVLIWDYLSPINLIATYPHLVVLGTGLAFGFLVGRMILAHLCDEPKGLKTN 318

Query: 72  MCM 75
           MCM
Sbjct: 319 MCM 321

[Close]

BLAST of PI0015536 vs. ExPASy Swiss-Prot
Match: O82568 (Choline/ethanolaminephosphotransferase 2 OS=Arabidopsis thaliana OX=3702 GN=AAPT2 PE=1 SV=1)

HSP 1 Score: 112.8 bits (281), Expect = 4.0e-24
Identity = 50/63 (79.37%), Postives = 55/63 (87.30%), Query Frame = 0


Query: 12  LYPFVVLVGGVLAWDHLSPSDIIGSYPHLVITGTGLAFGFLVGRMILAHLCDEPKGLKTG 71
           LYP V L+ GVL WD+LSP D+I +YPHLV+ GTGLAFGFLVGRMILAHLCDEPKGLKT 
Sbjct: 259 LYPLVTLIAGVLIWDYLSPIDLIRNYPHLVVLGTGLAFGFLVGRMILAHLCDEPKGLKTN 318

Query: 72  MCM 75
           MCM
Sbjct: 319 MCM 321

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BLAST of PI0015536 vs. ExPASy Swiss-Prot
Match: Q8I5R7 (Proline--tRNA ligase OS=Plasmodium falciparum (isolate 3D7) OX=36329 GN=proRS PE=1 SV=1)

HSP 1 Score: 84.0 bits (206), Expect = 2.0e-15
Identity = 49/130 (37.69%), Postives = 80/130 (61.54%), Query Frame = 0


Query: 65  PKGLKTGMCMVRAVRRDNSAK-DIPRDSLVEQVKELLESIQQSLFDAAKEKQDACIQVIN 124
           PK L+   C++  VRRDN+ K ++ ++S++ + +++L  I ++LF  AK+K D  I  + 
Sbjct: 603 PKDLQNNSCVI--VRRDNNEKCNVKKESVLLETQQMLVDIHKNLFLKAKKKLDDSIVQVT 662

Query: 125 TWEEFTEALGQKKMILAPWCD----EEEVEKDVK----TRTKGEV---GAAKTLCSPFEQ 182
           ++ E   AL +KKM+LAPWC+    EEE++K+ +     +T  E    GA K LC P +Q
Sbjct: 663 SFSEVMNALNKKKMVLAPWCEDIATEEEIKKETQRLSLNQTNSETTLSGAMKPLCIPLDQ 722

[Close]

BLAST of PI0015536 vs. ExPASy Swiss-Prot
Match: O60155 (Putative proline--tRNA ligase C19C7.06 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=prs1 PE=3 SV=1)

HSP 1 Score: 74.3 bits (181), Expect = 1.6e-12
Identity = 39/112 (34.82%), Postives = 63/112 (56.25%), Query Frame = 0


Query: 75  VRAVRRDNSAK-DIPRDSLVEQVKELLESIQQSLFDAAKEKQDACIQVINTWEEFTEALG 134
           V AVRRD   K  +P ++L + V +LL  IQ ++++ AK ++DA +  +  W +F  AL 
Sbjct: 575 VTAVRRDTFEKIPVPLNNLEKGVSDLLAKIQTNMYETAKAERDAHVVKVKEWADFVPALN 634

Query: 135 QKKMILAPWCDEEEVEKDVKTRTKGEVG------------AAKTLCSPFEQP 174
           +K +++ PWC+  E EK++K  +  +V              AK+LC P EQP
Sbjct: 635 KKNIVMIPWCNTTECEKEIKKNSARQVNGDEPEDEKAPSMGAKSLCIPLEQP 686

[Close]

BLAST of PI0015536 vs. ExPASy TrEMBL
Match: A0A1S4DS87 (Prolyl-tRNA synthetase OS=Cucumis melo OX=3656 GN=LOC103503756 PE=3 SV=1)

HSP 1 Score: 194.1 bits (492), Expect = 5.0e-46
Identity = 102/120 (85.00%), Postives = 107/120 (89.17%), Query Frame = 0


Query: 65  PKGLKTGMCMVRAVRRDNSA-KDIPRDSLVEQVKELLESIQQSLFDAAKEKQDACIQVIN 124
           PK L      VRAVRRDNSA KDIPR  LVEQVKELLESIQQSLFDAAKEK+DACIQV+N
Sbjct: 379 PKDLANN--QVRAVRRDNSAKKDIPRALLVEQVKELLESIQQSLFDAAKEKRDACIQVVN 438

Query: 125 TWEEFTEALGQKKMILAPWCDEEEVEKDVKTRTKGEVGAAKTLCSPFEQPPLPE-VVCFS 183
           TWEEFTEALGQKKMILAPWC+EEEVEKDVKTRTKGE+GAAKTLCSPFEQPPLPE   CF+
Sbjct: 439 TWEEFTEALGQKKMILAPWCNEEEVEKDVKTRTKGEMGAAKTLCSPFEQPPLPEGTKCFA 496

[Close]

BLAST of PI0015536 vs. ExPASy TrEMBL
Match: A0A5A7U355 (Prolyl-tRNA synthetase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold180G00130 PE=3 SV=1)

HSP 1 Score: 194.1 bits (492), Expect = 5.0e-46
Identity = 102/120 (85.00%), Postives = 107/120 (89.17%), Query Frame = 0


Query: 65  PKGLKTGMCMVRAVRRDNSA-KDIPRDSLVEQVKELLESIQQSLFDAAKEKQDACIQVIN 124
           PK L      VRAVRRDNSA KDIPR  LVEQVKELLESIQQSLFDAAKEK+DACIQV+N
Sbjct: 380 PKDLANN--QVRAVRRDNSAKKDIPRALLVEQVKELLESIQQSLFDAAKEKRDACIQVVN 439

Query: 125 TWEEFTEALGQKKMILAPWCDEEEVEKDVKTRTKGEVGAAKTLCSPFEQPPLPE-VVCFS 183
           TWEEFTEALGQKKMILAPWC+EEEVEKDVKTRTKGE+GAAKTLCSPFEQPPLPE   CF+
Sbjct: 440 TWEEFTEALGQKKMILAPWCNEEEVEKDVKTRTKGEMGAAKTLCSPFEQPPLPEGTKCFA 497

[Close]

BLAST of PI0015536 vs. ExPASy TrEMBL
Match: A0A1S3CR78 (Prolyl-tRNA synthetase OS=Cucumis melo OX=3656 GN=LOC103503756 PE=3 SV=1)

HSP 1 Score: 194.1 bits (492), Expect = 5.0e-46
Identity = 102/120 (85.00%), Postives = 107/120 (89.17%), Query Frame = 0


Query: 65  PKGLKTGMCMVRAVRRDNSA-KDIPRDSLVEQVKELLESIQQSLFDAAKEKQDACIQVIN 124
           PK L      VRAVRRDNSA KDIPR  LVEQVKELLESIQQSLFDAAKEK+DACIQV+N
Sbjct: 329 PKDLANN--QVRAVRRDNSAKKDIPRALLVEQVKELLESIQQSLFDAAKEKRDACIQVVN 388

Query: 125 TWEEFTEALGQKKMILAPWCDEEEVEKDVKTRTKGEVGAAKTLCSPFEQPPLPE-VVCFS 183
           TWEEFTEALGQKKMILAPWC+EEEVEKDVKTRTKGE+GAAKTLCSPFEQPPLPE   CF+
Sbjct: 389 TWEEFTEALGQKKMILAPWCNEEEVEKDVKTRTKGEMGAAKTLCSPFEQPPLPEGTKCFA 446

[Close]

BLAST of PI0015536 vs. ExPASy TrEMBL
Match: A0A0A0KTZ1 (Prolyl-tRNA synthetase OS=Cucumis sativus OX=3659 GN=Csa_4G015750 PE=3 SV=1)

HSP 1 Score: 191.8 bits (486), Expect = 2.5e-45
Identity = 101/120 (84.17%), Postives = 105/120 (87.50%), Query Frame = 0


Query: 65  PKGLKTGMCMVRAVRRDNSA-KDIPRDSLVEQVKELLESIQQSLFDAAKEKQDACIQVIN 124
           PK L      VRAVRRDNS  KDIPRDSLVEQVKELLESIQQSLFDAAK K+D CIQVIN
Sbjct: 379 PKDLANN--QVRAVRRDNSGKKDIPRDSLVEQVKELLESIQQSLFDAAKVKRDTCIQVIN 438

Query: 125 TWEEFTEALGQKKMILAPWCDEEEVEKDVKTRTKGEVGAAKTLCSPFEQPPLPE-VVCFS 183
           TWEEFTEALGQKKMILAPWCDEEEVEKDVKTRTKGE+GAAKTLCSPF+QP LPE   CF+
Sbjct: 439 TWEEFTEALGQKKMILAPWCDEEEVEKDVKTRTKGEMGAAKTLCSPFDQPALPEGTKCFA 496

[Close]

BLAST of PI0015536 vs. ExPASy TrEMBL
Match: A0A5A7TN35 (Prolyl-tRNA synthetase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold46G003510 PE=3 SV=1)

HSP 1 Score: 191.0 bits (484), Expect = 4.2e-45
Identity = 101/120 (84.17%), Postives = 105/120 (87.50%), Query Frame = 0


Query: 65  PKGLKTGMCMVRAVRRDNSA-KDIPRDSLVEQVKELLESIQQSLFDAAKEKQDACIQVIN 124
           PK L      VRAVRRDNSA KDIPRDSLVEQVKELLESIQQSLFDAAK K+D CIQVIN
Sbjct: 381 PKDLANN--QVRAVRRDNSAKKDIPRDSLVEQVKELLESIQQSLFDAAKVKRDTCIQVIN 440

Query: 125 TWEEFTEALGQKKMILAPWCDEEEVEKDVKTRTKGEVGAAKTLCSPFEQPPLPE-VVCFS 183
           TWEEFTEAL QKKMILAPWCDEEEVEKDVKTRTKGE+GAAKTLCSPF+QP LPE   CF+
Sbjct: 441 TWEEFTEALSQKKMILAPWCDEEEVEKDVKTRTKGEMGAAKTLCSPFDQPALPEGTKCFA 498

[Close]

BLAST of PI0015536 vs. NCBI nr
Match: XP_038907039.1 (proline--tRNA ligase, cytoplasmic-like isoform X2 [Benincasa hispida])

HSP 1 Score: 195.3 bits (495), Expect = 4.6e-46
Identity = 103/120 (85.83%), Postives = 106/120 (88.33%), Query Frame = 0


Query: 65  PKGLKTGMCMVRAVRRDNSA-KDIPRDSLVEQVKELLESIQQSLFDAAKEKQDACIQVIN 124
           PK L      VRAVRRDNS  KDIPRDSLVEQVKELLESIQQSLFDAAKEK+D CIQVIN
Sbjct: 329 PKDLANN--QVRAVRRDNSGKKDIPRDSLVEQVKELLESIQQSLFDAAKEKRDKCIQVIN 388

Query: 125 TWEEFTEALGQKKMILAPWCDEEEVEKDVKTRTKGEVGAAKTLCSPFEQPPLPE-VVCFS 183
           TWEEFTEALGQKKMILAPWCDEEEVEKDVKTRTKGE+GAAKTLCSPFEQP LPE   CF+
Sbjct: 389 TWEEFTEALGQKKMILAPWCDEEEVEKDVKTRTKGEMGAAKTLCSPFEQPALPEGTKCFA 446

[Close]

BLAST of PI0015536 vs. NCBI nr
Match: XP_038907011.1 (proline--tRNA ligase, cytoplasmic-like isoform X1 [Benincasa hispida] >XP_038907020.1 proline--tRNA ligase, cytoplasmic-like isoform X1 [Benincasa hispida] >XP_038907029.1 proline--tRNA ligase, cytoplasmic-like isoform X1 [Benincasa hispida])

HSP 1 Score: 195.3 bits (495), Expect = 4.6e-46
Identity = 103/120 (85.83%), Postives = 106/120 (88.33%), Query Frame = 0


Query: 65  PKGLKTGMCMVRAVRRDNSA-KDIPRDSLVEQVKELLESIQQSLFDAAKEKQDACIQVIN 124
           PK L      VRAVRRDNS  KDIPRDSLVEQVKELLESIQQSLFDAAKEK+D CIQVIN
Sbjct: 379 PKDLANN--QVRAVRRDNSGKKDIPRDSLVEQVKELLESIQQSLFDAAKEKRDKCIQVIN 438

Query: 125 TWEEFTEALGQKKMILAPWCDEEEVEKDVKTRTKGEVGAAKTLCSPFEQPPLPE-VVCFS 183
           TWEEFTEALGQKKMILAPWCDEEEVEKDVKTRTKGE+GAAKTLCSPFEQP LPE   CF+
Sbjct: 439 TWEEFTEALGQKKMILAPWCDEEEVEKDVKTRTKGEMGAAKTLCSPFEQPALPEGTKCFA 496

[Close]

BLAST of PI0015536 vs. NCBI nr
Match: XP_008466343.1 (PREDICTED: proline--tRNA ligase, cytoplasmic isoform X4 [Cucumis melo])

HSP 1 Score: 194.1 bits (492), Expect = 1.0e-45
Identity = 102/120 (85.00%), Postives = 107/120 (89.17%), Query Frame = 0


Query: 65  PKGLKTGMCMVRAVRRDNSA-KDIPRDSLVEQVKELLESIQQSLFDAAKEKQDACIQVIN 124
           PK L      VRAVRRDNSA KDIPR  LVEQVKELLESIQQSLFDAAKEK+DACIQV+N
Sbjct: 329 PKDLANN--QVRAVRRDNSAKKDIPRALLVEQVKELLESIQQSLFDAAKEKRDACIQVVN 388

Query: 125 TWEEFTEALGQKKMILAPWCDEEEVEKDVKTRTKGEVGAAKTLCSPFEQPPLPE-VVCFS 183
           TWEEFTEALGQKKMILAPWC+EEEVEKDVKTRTKGE+GAAKTLCSPFEQPPLPE   CF+
Sbjct: 389 TWEEFTEALGQKKMILAPWCNEEEVEKDVKTRTKGEMGAAKTLCSPFEQPPLPEGTKCFA 446

[Close]

BLAST of PI0015536 vs. NCBI nr
Match: XP_008466310.1 (PREDICTED: proline--tRNA ligase, cytoplasmic isoform X1 [Cucumis melo] >XP_008466350.1 PREDICTED: proline--tRNA ligase, cytoplasmic isoform X1 [Cucumis melo] >XP_016898851.1 PREDICTED: proline--tRNA ligase, cytoplasmic isoform X1 [Cucumis melo])

HSP 1 Score: 194.1 bits (492), Expect = 1.0e-45
Identity = 102/120 (85.00%), Postives = 107/120 (89.17%), Query Frame = 0


Query: 65  PKGLKTGMCMVRAVRRDNSA-KDIPRDSLVEQVKELLESIQQSLFDAAKEKQDACIQVIN 124
           PK L      VRAVRRDNSA KDIPR  LVEQVKELLESIQQSLFDAAKEK+DACIQV+N
Sbjct: 379 PKDLANN--QVRAVRRDNSAKKDIPRALLVEQVKELLESIQQSLFDAAKEKRDACIQVVN 438

Query: 125 TWEEFTEALGQKKMILAPWCDEEEVEKDVKTRTKGEVGAAKTLCSPFEQPPLPE-VVCFS 183
           TWEEFTEALGQKKMILAPWC+EEEVEKDVKTRTKGE+GAAKTLCSPFEQPPLPE   CF+
Sbjct: 439 TWEEFTEALGQKKMILAPWCNEEEVEKDVKTRTKGEMGAAKTLCSPFEQPPLPEGTKCFA 496

[Close]

BLAST of PI0015536 vs. NCBI nr
Match: KAA0050272.1 (proline--tRNA ligase [Cucumis melo var. makuwa] >TYJ98135.1 proline--tRNA ligase [Cucumis melo var. makuwa])

HSP 1 Score: 194.1 bits (492), Expect = 1.0e-45
Identity = 102/120 (85.00%), Postives = 107/120 (89.17%), Query Frame = 0


Query: 65  PKGLKTGMCMVRAVRRDNSA-KDIPRDSLVEQVKELLESIQQSLFDAAKEKQDACIQVIN 124
           PK L      VRAVRRDNSA KDIPR  LVEQVKELLESIQQSLFDAAKEK+DACIQV+N
Sbjct: 380 PKDLANN--QVRAVRRDNSAKKDIPRALLVEQVKELLESIQQSLFDAAKEKRDACIQVVN 439

Query: 125 TWEEFTEALGQKKMILAPWCDEEEVEKDVKTRTKGEVGAAKTLCSPFEQPPLPE-VVCFS 183
           TWEEFTEALGQKKMILAPWC+EEEVEKDVKTRTKGE+GAAKTLCSPFEQPPLPE   CF+
Sbjct: 440 TWEEFTEALGQKKMILAPWCNEEEVEKDVKTRTKGEMGAAKTLCSPFEQPPLPEGTKCFA 497

[Close]

BLAST of PI0015536 vs. TAIR 10
Match: AT3G62120.1 (Class II aaRS and biotin synthetases superfamily protein )

HSP 1 Score: 159.8 bits (403), Expect = 2.0e-39
Identity = 76/110 (69.09%), Postives = 93/110 (84.55%), Query Frame = 0


Query: 75  VRAVRRDNSAK-DIPRDSLVEQVKELLESIQQSLFDAAKEKQDACIQVINTWEEFTEALG 134
           VR VRRDN  K DIPR SLVE VKELLE IQQ++++ AK+K++AC+Q + TW+EF +AL 
Sbjct: 406 VRTVRRDNGVKEDIPRGSLVEHVKELLEKIQQNMYEVAKQKREACVQEVKTWDEFIKALN 465

Query: 135 QKKMILAPWCDEEEVEKDVKTRTKGEVGAAKTLCSPFEQPPLPE-VVCFS 183
           +KK+ILAPWCDEEEVE+DVK RTKGE GAAKTLCSPF+QP LPE  +CF+
Sbjct: 466 EKKLILAPWCDEEEVERDVKARTKGETGAAKTLCSPFDQPELPEGTLCFA 515

[Close]

BLAST of PI0015536 vs. TAIR 10
Match: AT3G62120.2 (Class II aaRS and biotin synthetases superfamily protein )

HSP 1 Score: 159.8 bits (403), Expect = 2.0e-39
Identity = 76/110 (69.09%), Postives = 93/110 (84.55%), Query Frame = 0


Query: 75  VRAVRRDNSAK-DIPRDSLVEQVKELLESIQQSLFDAAKEKQDACIQVINTWEEFTEALG 134
           VR VRRDN  K DIPR SLVE VKELLE IQQ++++ AK+K++AC+Q + TW+EF +AL 
Sbjct: 406 VRTVRRDNGVKEDIPRGSLVEHVKELLEKIQQNMYEVAKQKREACVQEVKTWDEFIKALN 465

Query: 135 QKKMILAPWCDEEEVEKDVKTRTKGEVGAAKTLCSPFEQPPLPE-VVCFS 183
           +KK+ILAPWCDEEEVE+DVK RTKGE GAAKTLCSPF+QP LPE  +CF+
Sbjct: 466 EKKLILAPWCDEEEVERDVKARTKGETGAAKTLCSPFDQPELPEGTLCFA 515

[Close]

BLAST of PI0015536 vs. TAIR 10
Match: AT5G10880.1 (tRNA synthetase-related / tRNA ligase-related )

HSP 1 Score: 132.5 bits (332), Expect = 3.4e-31
Identity = 68/115 (59.13%), Postives = 83/115 (72.17%), Query Frame = 0


Query: 75  VRAVRRDNSAK-DIPRDSLVEQVKELLESIQQSLFDAAKEKQDACIQVINTWEEFTEALG 134
           VR V RDN AK D+ R  L+EQVK+LLE IQ +L+D AK K + C Q + TW+EF EAL 
Sbjct: 180 VRIVTRDNGAKMDVKRGDLIEQVKDLLEKIQSNLYDVAKRKVEECTQKVETWDEFVEALS 239

Query: 135 QKKMILAPWCDEEEVEKDVKTRTKGEV-----GAAKTLCSPFEQPPL-PEVVCFS 183
           QKK+ILAPWCD+ EVEKDVK RT+G+      G AKTLC+P EQP L  E +CF+
Sbjct: 240 QKKLILAPWCDKVEVEKDVKRRTRGDETGGGGGGAKTLCTPLEQPELGEETLCFA 294

[Close]

BLAST of PI0015536 vs. TAIR 10
Match: AT1G13560.2 (aminoalcoholphosphotransferase 1 )

HSP 1 Score: 117.1 bits (292), Expect = 1.5e-26
Identity = 52/63 (82.54%), Postives = 58/63 (92.06%), Query Frame = 0


Query: 12  LYPFVVLVGGVLAWDHLSPSDIIGSYPHLVITGTGLAFGFLVGRMILAHLCDEPKGLKTG 71
           LYPFVVL+GGVL WD+LSP ++I +YPHLV+ GTGLAFGFLVGRMILAHLCDEPKGLKT 
Sbjct: 216 LYPFVVLLGGVLIWDYLSPINLIATYPHLVVLGTGLAFGFLVGRMILAHLCDEPKGLKTN 275

Query: 72  MCM 75
           MCM
Sbjct: 276 MCM 278

[Close]

BLAST of PI0015536 vs. TAIR 10
Match: AT1G13560.1 (aminoalcoholphosphotransferase 1 )

HSP 1 Score: 117.1 bits (292), Expect = 1.5e-26
Identity = 52/63 (82.54%), Postives = 58/63 (92.06%), Query Frame = 0


Query: 12  LYPFVVLVGGVLAWDHLSPSDIIGSYPHLVITGTGLAFGFLVGRMILAHLCDEPKGLKTG 71
           LYPFVVL+GGVL WD+LSP ++I +YPHLV+ GTGLAFGFLVGRMILAHLCDEPKGLKT 
Sbjct: 259 LYPFVVLLGGVLIWDYLSPINLIATYPHLVVLGTGLAFGFLVGRMILAHLCDEPKGLKTN 318

Query: 72  MCM 75
           MCM
Sbjct: 319 MCM 321

The following BLAST results are available for this feature:

BLAST of PI0015536 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9M1R2	2.8e-38	69.09	Proline--tRNA ligase, cytoplasmic OS=Arabidopsis thaliana OX=3702 GN=At3g62120 P...	[more]
O82567	2.1e-25	82.54	Choline/ethanolaminephosphotransferase 1 OS=Arabidopsis thaliana OX=3702 GN=AAPT...	[more]
O82568	4.0e-24	79.37	Choline/ethanolaminephosphotransferase 2 OS=Arabidopsis thaliana OX=3702 GN=AAPT...	[more]
Q8I5R7	2.0e-15	37.69	Proline--tRNA ligase OS=Plasmodium falciparum (isolate 3D7) OX=36329 GN=proRS PE...	[more]
O60155	1.6e-12	34.82	Putative proline--tRNA ligase C19C7.06 OS=Schizosaccharomyces pombe (strain 972 ...	[more]

BLAST of PI0015536 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S4DS87	5.0e-46	85.00	Prolyl-tRNA synthetase OS=Cucumis melo OX=3656 GN=LOC103503756 PE=3 SV=1	[more]
A0A5A7U355	5.0e-46	85.00	Prolyl-tRNA synthetase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1...	[more]
A0A1S3CR78	5.0e-46	85.00	Prolyl-tRNA synthetase OS=Cucumis melo OX=3656 GN=LOC103503756 PE=3 SV=1	[more]
A0A0A0KTZ1	2.5e-45	84.17	Prolyl-tRNA synthetase OS=Cucumis sativus OX=3659 GN=Csa_4G015750 PE=3 SV=1	[more]
A0A5A7TN35	4.2e-45	84.17	Prolyl-tRNA synthetase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold4...	[more]

BLAST of PI0015536 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038907039.1	4.6e-46	85.83	proline--tRNA ligase, cytoplasmic-like isoform X2 [Benincasa hispida]	[more]
XP_038907011.1	4.6e-46	85.83	proline--tRNA ligase, cytoplasmic-like isoform X1 [Benincasa hispida] >XP_038907...	[more]
XP_008466343.1	1.0e-45	85.00	PREDICTED: proline--tRNA ligase, cytoplasmic isoform X4 [Cucumis melo]	[more]
XP_008466310.1	1.0e-45	85.00	PREDICTED: proline--tRNA ligase, cytoplasmic isoform X1 [Cucumis melo] >XP_00846...	[more]
KAA0050272.1	1.0e-45	85.00	proline--tRNA ligase [Cucumis melo var. makuwa] >TYJ98135.1 proline--tRNA ligase...	[more]

BLAST of PI0015536 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G62120.1	2.0e-39	69.09	Class II aaRS and biotin synthetases superfamily protein	[more]
AT3G62120.2	2.0e-39	69.09	Class II aaRS and biotin synthetases superfamily protein	[more]
AT5G10880.1	3.4e-31	59.13	tRNA synthetase-related / tRNA ligase-related	[more]
AT1G13560.2	1.5e-26	82.54	aminoalcoholphosphotransferase 1	[more]
AT1G13560.1	1.5e-26	82.54	aminoalcoholphosphotransferase 1	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR016061	Proline-tRNA ligase, class II, C-terminal	SMART	SM00946	ProRS_C_1_2	coord: 125..181 e-value: 3.9E-7 score: 39.7
IPR016061	Proline-tRNA ligase, class II, C-terminal	PFAM	PF09180	ProRS-C_1	coord: 125..181 e-value: 8.5E-15 score: 54.8
IPR017449	Prolyl-tRNA synthetase, class II	GENE3D	3.30.110.30		coord: 119..182 e-value: 2.2E-16 score: 61.8
IPR017449	Prolyl-tRNA synthetase, class II	SUPERFAMILY	64586	C-terminal domain of ProRS	coord: 120..181
None	No IPR available	PANTHER	PTHR43382:SF4	PROLINE--TRNA LIGASE, CYTOPLASMIC-LIKE	coord: 74..181
IPR004499	Proline-tRNA ligase, class IIa, archaeal-type	PANTHER	PTHR43382	PROLYL-TRNA SYNTHETASE	coord: 74..181

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
PI0015536.1	PI0015536.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006433	prolyl-tRNA aminoacylation
cellular_component	GO:0017101	aminoacyl-tRNA synthetase multienzyme complex
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0004827	proline-tRNA ligase activity
molecular_function	GO:0000166	nucleotide binding