PI0015130 (gene) Melon (PI 482460) v1

Overview
NamePI0015130
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
DescriptionGlutamyl-tRNA(Gln) amidotransferase subunit A
Locationchr11: 28369668 .. 28372543 (+)
RNA-Seq ExpressionPI0015130
SyntenyPI0015130
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGCAGAGCTGCCATGGCTGCCCAAAGCTTTCACATTTACATGTTCATGGTTCTGATTTTATCATCATATGGGAGTTGCAGCTTTGACACTGACCTCTCCATTGAAGAAGCAACTTTGAAAGATCTCCAACTTGCTTTTTACAAAAACAAACTCACCTCCAAGCAACTTGTTGAGTTTTACCTCCAACAAGTGGGTAGATTTAACCCCATTTTGAAAGGGATCATAGAAGTGAATCCAGATGCTTTAGACCAAGCCTTTCGAGCCGACATCGAGCGCAAGAGAACCTCGCCTAGCTCTTTGTCTCCATTGCATGGCATTCCTGTACTTGTAAAAGATAACATTGGAACCAAGGATAAGCTGAACACAACGGCTGGCTCTTTTGCTCTGCTTGGTTCTATTGTTCCTCGTGATGCAGGTGTGGTGACCAAGTTGAGGAAGGCAGGTGCAATTATCTTTGGAAAGGCGAGCCTGAGCGAGTGGTCTTACTTCAGGTCCTCCGGTCTCCCCAATGGTTGGAGCGCTAGGGGTGGCCAAGGCAAGGTGTGTAACTTTTCCAACTTTTTCAATAATTAGCTAAAACCAGACCAAACTGAACCATGAACATTGTGAGGTTCTTTTGATTATTTCAGAGATAGTGCCTCTTTTAAGTTCATCTTTGAACTTGATCCAATTTTTGTTTTCAAACCAAATGTTCCATTAGATATTGTATTAAATACACCTTCACCTACTAGATGAAGCTTTTGAGTTAATTAATGATTTACGATGGTGTTAGAGCATAGTTTTGTTTACATTCTCGTGGAATATGGTTCTTAAATCACCAATTGACTCAAAAGTTTAAGCCAGTAGGTGAAGGTAAATTTAATATAATATATAACATGTTTATTTAAGGTTTGACTCTGAAACCATGTTAAATCACTGACCCAAAAGCTTAAGTTGAACGGTTTTGTATACTCATATTTAGCCCATAAACAATCCTAATTCAAAATATGCAGTTAACATCAGCGTCTAAGCTAACTTTTAAATCAAAAGCAGTCAGTTAGAAAGTCCAATTAAAAGACAACAAACTAAGCACACTTTTTTCACTTTGCAGAATCCTTACACACTAGGAGAACCTTGTGGATCGAGTAGCGGCTCCGCCATATCGGTTGCAGCAAATATGGTTGCAGTTTCACTAGGAACTGACACTGATGGATCAATATTATGCCCTTCTACTTTCAACTCAGTAGTTGGCATCAGACCAACAGTAGGTCTCACTAGTCGAGCAGGGGTTGTTCCAATCTCTTTAAGGCAGGATGCTATCGGGTAATTAATCAATTTGTTTCTATTAAAATGTTACCATATATATTAATATCCAAACGAAGTACTTTCCGCTGAATGAGTAATGATGGAACAACACAGGCCTATTTGTAGAACAGTATCAGATGCTGCTTATGTTCTAGATGCTATTGTAGGAGTGGACAGATATGATAATTCAACAATTGAAGCATCAAAATACATTCCAAAAGGTGGATATGGCCAATTTTTAAGGGTTGATGGGCTGAAAGGAAAGAGAATAGGAATTGTGAGGAAATTCTTTGATTTTGGCCATGATGATACCTTCTACAGTGGAGCTTTTGAGAAAGTTTTCGAAACCCTGAAGTAAGAAAACAAACTCACTTTCCATAACAATTCATGTCGTTACATGTTTTTATCAATGTTTGAAAAGGCGGGCTTGAGGCTTTTCCTCTCCAAGAGGTGAGGCGTAAGCCTCAAATAATGAATTAAATACATATCACAAAACCTTAATTGGTATGATCAAGTATTGCTAAACATGATAATCATATAAAAGTGTAAAAAAGAAACTTCACCCCCACAATTTTCCCTTGATAAAAAATTCTAAAAGGACATCTTTATTATTGCTATTATTACTAATAGGATTGATTTATACTATATATTTTTGCTTTCAGCATTCTTTTCACAAGAGTAAAAATAGTCTTAGTTCAAATTGCCATAGCTCCAACTATAATATTTTTCAAAAATTAGGCAAATCATGATTATTTTTCGGTGAGGCATAGAGATTTTGCGTTTTTGCAAAAGCGTAAACCTGTTATGCTTCAACGAAGGTGTAAGTCTTAAGGCTAAAACTTAGAGCCTTGCCTCGAGGCGAGCCTCAAGGCGTAAGCCTCAAAATAGCTTTTTTTAAAACATTGGTTTTTATATGACCCTTTAATCTTCAATAACATGAAAGGCAAGGAGGAGCAATATTGGTGGACAATCTGATAATAGACAACCTACATGTAATCATTGGTGGTTCAAGTGGAGAGTGGACTGCATTGCCTGCTGAATTCAAAATATCCTTAAATGAATATCTCAAAGAGCTAGTTGCTTCTCCAATTCGATCCTTATCAGATGCAATAGAATTCAACAAAAAGAACTCAAAACTTGTGAGCAATTATCATCTATTCTGTCGTAATTGTATACATAGGATAGCGAAAAATAGACTATCATAACAATGAAATCTTATCACTATGTAGGAAAAGCTAAAGGAGTATGGTCAGAAGTTATTTTTAAAAGCAGAAGCCACAAATGGAATTGGAGATGAAGAAAAGGCAGCATTAGCCAGAGTAGGATCATTGTCGAAAAACGGATTAGAGAGAGTAATGATTAAGAATAAGCTTGATGCAATCGCAGCTCCTGGTTGGTTAATCTCTCCTGTTCTTGCAATTGGAGGTTTTCCTGGAGTTAGTGTACCAGCTGGATATGAACCTGAAGGGGTTCCCTATGGCGTTGGCTTTGGAGGATTAAAAGGGTTTGAGCCAAGGCTGATAGAGATTGCTTATGGATTTGAGCAGTTGACTAAGAGTAGAAAGCCCCCTTCAATTAAGAGACGTTAA

mRNA sequence

ATGAGCAGAGCTGCCATGGCTGCCCAAAGCTTTCACATTTACATGTTCATGGTTCTGATTTTATCATCATATGGGAGTTGCAGCTTTGACACTGACCTCTCCATTGAAGAAGCAACTTTGAAAGATCTCCAACTTGCTTTTTACAAAAACAAACTCACCTCCAAGCAACTTGTTGAGTTTTACCTCCAACAAGTGGGTAGATTTAACCCCATTTTGAAAGGGATCATAGAAGTGAATCCAGATGCTTTAGACCAAGCCTTTCGAGCCGACATCGAGCGCAAGAGAACCTCGCCTAGCTCTTTGTCTCCATTGCATGGCATTCCTGTACTTGTAAAAGATAACATTGGAACCAAGGATAAGCTGAACACAACGGCTGGCTCTTTTGCTCTGCTTGGTTCTATTGTTCCTCGTGATGCAGGTGTGGTGACCAAGTTGAGGAAGGCAGGTGCAATTATCTTTGGAAAGGCGAGCCTGAGCGAGTGGTCTTACTTCAGGTCCTCCGGTCTCCCCAATGGTTGGAGCGCTAGGGGTGGCCAAGGCAAGAATCCTTACACACTAGGAGAACCTTGTGGATCGAGTAGCGGCTCCGCCATATCGGTTGCAGCAAATATGGTTGCAGTTTCACTAGGAACTGACACTGATGGATCAATATTATGCCCTTCTACTTTCAACTCAGTAGTTGGCATCAGACCAACAGTAGGTCTCACTAGTCGAGCAGGGGTTGTTCCAATCTCTTTAAGGCAGGATGCTATCGGGCCTATTTGTAGAACAGTATCAGATGCTGCTTATGTTCTAGATGCTATTGTAGGAGTGGACAGATATGATAATTCAACAATTGAAGCATCAAAATACATTCCAAAAGGTGGATATGGCCAATTTTTAAGGGTTGATGGGCTGAAAGGAAAGAGAATAGGAATTGTGAGGAAATTCTTTGATTTTGGCCATGATGATACCTTCTACAGTGGAGCTTTTGAGAAAGTTTTCGAAACCCTGAAGCAAGGAGGAGCAATATTGGTGGACAATCTGATAATAGACAACCTACATGTAATCATTGGTGGTTCAAGTGGAGAGTGGACTGCATTGCCTGCTGAATTCAAAATATCCTTAAATGAATATCTCAAAGAGCTAGTTGCTTCTCCAATTCGATCCTTATCAGATGCAATAGAATTCAACAAAAAGAACTCAAAACTTGAAAAGCTAAAGGAGTATGGTCAGAAGTTATTTTTAAAAGCAGAAGCCACAAATGGAATTGGAGATGAAGAAAAGGCAGCATTAGCCAGAGTAGGATCATTGTCGAAAAACGGATTAGAGAGAGTAATGATTAAGAATAAGCTTGATGCAATCGCAGCTCCTGGTTGGTTAATCTCTCCTGTTCTTGCAATTGGAGGTTTTCCTGGAGTTAGTGTACCAGCTGGATATGAACCTGAAGGGGTTCCCTATGGCGTTGGCTTTGGAGGATTAAAAGGGTTTGAGCCAAGGCTGATAGAGATTGCTTATGGATTTGAGCAGTTGACTAAGAGTAGAAAGCCCCCTTCAATTAAGAGACGTTAA

Coding sequence (CDS)

ATGAGCAGAGCTGCCATGGCTGCCCAAAGCTTTCACATTTACATGTTCATGGTTCTGATTTTATCATCATATGGGAGTTGCAGCTTTGACACTGACCTCTCCATTGAAGAAGCAACTTTGAAAGATCTCCAACTTGCTTTTTACAAAAACAAACTCACCTCCAAGCAACTTGTTGAGTTTTACCTCCAACAAGTGGGTAGATTTAACCCCATTTTGAAAGGGATCATAGAAGTGAATCCAGATGCTTTAGACCAAGCCTTTCGAGCCGACATCGAGCGCAAGAGAACCTCGCCTAGCTCTTTGTCTCCATTGCATGGCATTCCTGTACTTGTAAAAGATAACATTGGAACCAAGGATAAGCTGAACACAACGGCTGGCTCTTTTGCTCTGCTTGGTTCTATTGTTCCTCGTGATGCAGGTGTGGTGACCAAGTTGAGGAAGGCAGGTGCAATTATCTTTGGAAAGGCGAGCCTGAGCGAGTGGTCTTACTTCAGGTCCTCCGGTCTCCCCAATGGTTGGAGCGCTAGGGGTGGCCAAGGCAAGAATCCTTACACACTAGGAGAACCTTGTGGATCGAGTAGCGGCTCCGCCATATCGGTTGCAGCAAATATGGTTGCAGTTTCACTAGGAACTGACACTGATGGATCAATATTATGCCCTTCTACTTTCAACTCAGTAGTTGGCATCAGACCAACAGTAGGTCTCACTAGTCGAGCAGGGGTTGTTCCAATCTCTTTAAGGCAGGATGCTATCGGGCCTATTTGTAGAACAGTATCAGATGCTGCTTATGTTCTAGATGCTATTGTAGGAGTGGACAGATATGATAATTCAACAATTGAAGCATCAAAATACATTCCAAAAGGTGGATATGGCCAATTTTTAAGGGTTGATGGGCTGAAAGGAAAGAGAATAGGAATTGTGAGGAAATTCTTTGATTTTGGCCATGATGATACCTTCTACAGTGGAGCTTTTGAGAAAGTTTTCGAAACCCTGAAGCAAGGAGGAGCAATATTGGTGGACAATCTGATAATAGACAACCTACATGTAATCATTGGTGGTTCAAGTGGAGAGTGGACTGCATTGCCTGCTGAATTCAAAATATCCTTAAATGAATATCTCAAAGAGCTAGTTGCTTCTCCAATTCGATCCTTATCAGATGCAATAGAATTCAACAAAAAGAACTCAAAACTTGAAAAGCTAAAGGAGTATGGTCAGAAGTTATTTTTAAAAGCAGAAGCCACAAATGGAATTGGAGATGAAGAAAAGGCAGCATTAGCCAGAGTAGGATCATTGTCGAAAAACGGATTAGAGAGAGTAATGATTAAGAATAAGCTTGATGCAATCGCAGCTCCTGGTTGGTTAATCTCTCCTGTTCTTGCAATTGGAGGTTTTCCTGGAGTTAGTGTACCAGCTGGATATGAACCTGAAGGGGTTCCCTATGGCGTTGGCTTTGGAGGATTAAAAGGGTTTGAGCCAAGGCTGATAGAGATTGCTTATGGATTTGAGCAGTTGACTAAGAGTAGAAAGCCCCCTTCAATTAAGAGACGTTAA

Protein sequence

MSRAAMAAQSFHIYMFMVLILSSYGSCSFDTDLSIEEATLKDLQLAFYKNKLTSKQLVEFYLQQVGRFNPILKGIIEVNPDALDQAFRADIERKRTSPSSLSPLHGIPVLVKDNIGTKDKLNTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWSYFRSSGLPNGWSARGGQGKNPYTLGEPCGSSSGSAISVAANMVAVSLGTDTDGSILCPSTFNSVVGIRPTVGLTSRAGVVPISLRQDAIGPICRTVSDAAYVLDAIVGVDRYDNSTIEASKYIPKGGYGQFLRVDGLKGKRIGIVRKFFDFGHDDTFYSGAFEKVFETLKQGGAILVDNLIIDNLHVIIGGSSGEWTALPAEFKISLNEYLKELVASPIRSLSDAIEFNKKNSKLEKLKEYGQKLFLKAEATNGIGDEEKAALARVGSLSKNGLERVMIKNKLDAIAAPGWLISPVLAIGGFPGVSVPAGYEPEGVPYGVGFGGLKGFEPRLIEIAYGFEQLTKSRKPPSIKRR
Homology
BLAST of PI0015130 vs. ExPASy Swiss-Prot
Match: A0A1P8B760 (Probable amidase At4g34880 OS=Arabidopsis thaliana OX=3702 GN=At4g34880 PE=2 SV=1)

HSP 1 Score: 572.8 bits (1475), Expect = 4.0e-162
Identity = 307/511 (60.08%), Postives = 395/511 (77.30%), Query Frame = 0

Query: 11  FHIYMFMVLILS-----SYGSCS---FDTDLSIEEATLKDLQLAFYKNKLTSKQLVEFYL 70
           F   + ++LILS     S GS S     +  SI+EAT++D+++AF + +LTSKQLVE YL
Sbjct: 8   FFSSLLILLILSQALIMSVGSASQIRLSSTFSIQEATIEDIRVAFNEKRLTSKQLVELYL 67

Query: 71  QQVGRFNPILKGIIEVNPDALDQAFRADIERKRTSPSSLSPLHGIPVLVKDNIGTKDKLN 130
           + + + NPIL  +IE NPDAL QA  AD ER   + + L  LHG+PVL+KD+I TKDKLN
Sbjct: 68  EAISKLNPILHAVIETNPDALIQAEIADRERDLKNTTKLPILHGVPVLLKDSISTKDKLN 127

Query: 131 TTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWSYFRSSGLPNGWSARGGQGKN 190
           TTAGSFALLGS+V RDAGVV +LR++GA+I GKASLSEW++FRS  +P+GWSARG QGKN
Sbjct: 128 TTAGSFALLGSVVARDAGVVKRLRESGAVILGKASLSEWAHFRSFSIPDGWSARGLQGKN 187

Query: 191 PYTL-GEPCGSSSGSAISVAANMVAVSLGTDTDGSILCPSTFNSVVGIRPTVGLTSRAGV 250
           PY L   P GSSSGSAISV AN+VAVSLGT+TDGSIL P++ NSVVGI+P+VGLTSRAGV
Sbjct: 188 PYVLSANPSGSSSGSAISVTANLVAVSLGTETDGSILSPASQNSVVGIKPSVGLTSRAGV 247

Query: 251 VPISLRQDAIGPICRTVSDAAYVLDAIVGVDRYDNSTIEASKYIPKGGYGQFLRVDGLKG 310
           VPISLRQD+IGPICRTVSDA ++LDAIVG D  D +T  AS++IP+GGY QFL   GLKG
Sbjct: 248 VPISLRQDSIGPICRTVSDAVHLLDAIVGYDPLDEATKTASEFIPEGGYKQFLTTSGLKG 307

Query: 311 KRIGIVRKFFDFGHDDTFYSGAFEKVFETLKQGGAILVDNLIIDNLHVIIGGS-SGEWTA 370
           KR+GIV K          +S   +   +TL++ GAI+++NL I N+ VI+GG+ SGE  A
Sbjct: 308 KRLGIVMK----------HSSLLDHHIKTLRREGAIVINNLTIPNIEVIVGGTDSGEEIA 367

Query: 371 LPAEFKISLNEYLKELVASPIRSLSDAIEFNKKNSKLEKLKEYGQKLFLKAEATNGIGDE 430
           L AEFK+SLN YLKELV SP+RSL+D I +N++ ++ EK+KE+GQ++FL AEAT+G+G++
Sbjct: 368 LLAEFKMSLNAYLKELVKSPVRSLADVIAYNEEFAEQEKVKEWGQEVFLTAEATSGMGEK 427

Query: 431 EKAALARVGSLSKNGLERVMIKNKLDAIAAPGWLISPVLAIGGFPGVSVPAGYEPEGVPY 490
           EK AL ++  LS+NG+E+++ +NKLDAI   G  +S VLAIGG+PG++VPAGY+  GVPY
Sbjct: 428 EKTALQKMKELSRNGIEKLIEENKLDAIVTLGSDLSSVLAIGGYPGINVPAGYDSGGVPY 487

Query: 491 GVGFGGLKGFEPRLIEIAYGFEQLTKSRKPP 512
           G+ FGGL+  EP+LIEIA+ FEQ T  RKPP
Sbjct: 488 GISFGGLRFSEPKLIEIAFAFEQATLIRKPP 508

BLAST of PI0015130 vs. ExPASy Swiss-Prot
Match: Q9URY4 (Putative amidase C869.01 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC869.01 PE=3 SV=1)

HSP 1 Score: 291.2 bits (744), Expect = 2.3e-77
Identity = 196/514 (38.13%), Postives = 293/514 (57.00%), Query Frame = 0

Query: 32  DLSIEEATLKDLQLAFYKNKLTSKQLVEFYLQQVGRFNPILKGIIEVNPDALDQAFRADI 91
           ++++E+AT+  LQ       LTS  +V  YL +  + NP + GI+++NPD L  A  +++
Sbjct: 63  NITLEDATIDQLQNYMENGILTSTDIVHCYLDRYLQVNPYVNGILQLNPDVLTIA--SEL 122

Query: 92  ERKRTSPSSLSPLHGIPVLVKDNIGTKDKLNTTAGSFALLGSIVPRDAGVVTKLRKAGAI 151
           + +R +     PLHGIP +VKDN  TKDK++TTAGS+ALLGSIVPRDA VV +LR+AGA+
Sbjct: 123 DDERANGIIRGPLHGIPFIVKDNFATKDKMDTTAGSYALLGSIVPRDAYVVKQLREAGAV 182

Query: 152 IFGKASLSEWSYFRSSGLPNGWSARGGQGKNPYTLG-EPCGSSSGSAISVAANMVAVSLG 211
           +FG A+LSEW+  RS+    G+SARGGQ + P+ L   P GSSSGSAISVA+NM+A +LG
Sbjct: 183 LFGHATLSEWADMRSNDYSEGYSARGGQSRCPFNLTVNPGGSSSGSAISVASNMIAFALG 242

Query: 212 TDTDGSILCPSTFNSVVGIRPTVGLTSRAGVVPISLRQDAIGPICRTVSDAAYVLDAIVG 271
           T+TDGSI+ P+  N VVG++PTVGLTSR GV+P S  QD  GPI RTV DA YV  ++ G
Sbjct: 243 TETDGSIIDPAMRNGVVGLKPTVGLTSRYGVIPESEHQDTTGPIARTVRDAVYVFQSMWG 302

Query: 272 VDRYDNSTIEASKYIPK-GGYGQFL-RVDGLKGKRIGIV-RKFFDFGHDDTFYSGAFEKV 331
           +D  D  T+  +   P+ G Y +FL     L+G R G+  ++ +     D        +V
Sbjct: 303 IDENDIYTLNQTGKTPEDGDYMKFLSNKTSLEGARFGLPWKRLWQNAKADEI--DRLLEV 362

Query: 332 FETLKQGGAILVDNLIIDNLHVI--------IGG-SSGEWTALPAEFKISLNEYLKELVA 391
            + +++ GAI+ +N    NL VI        +G  +  E+T +  +F  ++  YL E+  
Sbjct: 363 VKQIEEAGAIVYNNTNFYNLDVISNDGWNWELGSVNESEYTVVKVDFYNNIKSYLSEVKN 422

Query: 392 SPIRSLSDAIEFNKKNSKLEKLK-------EYGQKLFLKAEATNGIGDEEK-AALARVGS 451
           + I SL D +E+N K    E  K         GQ  FL +    G+ +E    A+  V  
Sbjct: 423 TEIHSLEDIVEYNNKYMGTEGGKPNIVPAFSSGQDGFLASLEWGGVKNETYWQAVEYVRR 482

Query: 452 LSKN-GLERVMIKNKLDAIAAPGWLISPVL------------AIGGFPGVSVPAGYEPEG 511
            S++ G++  +  N  D      ++++ +L            A  G+P +++P G +  G
Sbjct: 483 TSQDEGIDYAL--NYTDPKTNDSFILNGLLVPSGTSITYQQAAKAGYPMITLPIGVKTNG 542

BLAST of PI0015130 vs. ExPASy Swiss-Prot
Match: D4B3C8 (Putative amidase ARB_02965 OS=Arthroderma benhamiae (strain ATCC MYA-4681 / CBS 112371) OX=663331 GN=ARB_02965 PE=1 SV=1)

HSP 1 Score: 250.8 bits (639), Expect = 3.5e-65
Identity = 174/496 (35.08%), Postives = 266/496 (53.63%), Query Frame = 0

Query: 43  LQLAFYKNKLTSKQLVEFYLQQVGRFNPILKGIIEVNPDALDQAFRADIERKRTSPSSLS 102
           LQ  + +  +    +V+ Y+ ++   N  ++ + E+NPDAL  A + D ERK        
Sbjct: 94  LQTPYRQGNVLIIVVVQTYVARIAEVNSTVRAVTEINPDALTIAKQMDNERK--MGKLRG 153

Query: 103 PLHGIPVLVKDNIGTKDKLNTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWS 162
           PLHG+P+++K+NI T DK+++TAGS+A+ G+    DA V TKLR+AG +I GK+  S+W+
Sbjct: 154 PLHGLPIVIKNNIFTDDKMSSTAGSYAIFGARTSADATVATKLREAGLVIMGKSGASQWA 213

Query: 163 YFRSSGLPNGWSARGGQGKNPYTLG-EPCGSSSGSAISVAANMVAVSLGTDTDGSILCPS 222
            FRS    NGWSA GGQ    Y    +P GSSSGS ++    +   +LGT+T GSI+ P+
Sbjct: 214 NFRSLNSTNGWSAYGGQVTAAYIKNQDPSGSSSGSGVASDLGLAFATLGTETSGSIVSPA 273

Query: 223 TFNSVVGIRPTVGLTSRAGVVPISLRQDAIGPICRTVSDAAYVLDAIVGVDRYDNSTIEA 282
             +++VG++PTVGLTSR  VVPIS RQD +GP+ R+V DAAY+L  I G D  DN T  A
Sbjct: 274 DKSNIVGLKPTVGLTSRRFVVPISERQDTVGPMARSVKDAAYLLQVIAGKDSNDNYT-SA 333

Query: 283 SKYIPKGGYGQFLRVDGLKGKRIGIVRKFFD-FGHDDTFYSGAFEKVFETLKQGGAILVD 342
             +     Y +   ++ LKGKRIG+ R     FG   T     F +    +K+ GAI+V+
Sbjct: 334 IPFDTIPDYVKACDINALKGKRIGVPRNVIKIFGSPQTVVD-QFNQALAVMKKAGAIIVE 393

Query: 343 NLIIDNLHVIIGGSSGEWTALPAEFKISLNEYLKELVASPIRSLSDAIEFNKKNSKLEKL 402
           N    +          +   L A+   +L  + K+L  +P  +++D +E  ++ ++  +L
Sbjct: 394 NTDFTSFAEFAQSPIPD-DILYADSLTNLPAFFKQLKVNP-HNITD-LESLRRFTQHHRL 453

Query: 403 KEYGQK------LFLKAEATNGIGDEEKAALARVGSLSKNGLERVMIKNKLDAIAAPGWL 462
           +EY  +      + L+    N            V   ++ G+   + ++KLDA   P  L
Sbjct: 454 EEYPSRDTARWDIALQKGIKNTDPKFWPMYQKNVKFGNEGGILGALRRHKLDAAVLPTDL 513

Query: 463 ISPVLAIGGFPGVSVPAGYEPE---------------GVPYGVGFGGLKGFEPRLIEIAY 516
              + A+ G P ++VP G  P                G+P G+GF G    E +LI +AY
Sbjct: 514 SPYIPALIGSPIITVPMGVYPNGTKVNHDRELVTSGPGIPIGIGFMGDLWSEEKLIGLAY 573

BLAST of PI0015130 vs. ExPASy Swiss-Prot
Match: B0K3S3 (Glutamyl-tRNA(Gln) amidotransferase subunit A OS=Thermoanaerobacter sp. (strain X514) OX=399726 GN=gatA PE=3 SV=1)

HSP 1 Score: 176.8 bits (447), Expect = 6.3e-43
Identity = 162/524 (30.92%), Postives = 258/524 (49.24%), Query Frame = 0

Query: 33  LSIEEATLKDLQLAFYKNKLTSKQLVEFYLQQVGRFNPILKGIIEVNPD-ALDQAFRADI 92
           + +   T+ +L+    K ++++ ++ + YL+++    P +  ++ +  D AL +A  AD 
Sbjct: 1   MELYSLTIHELRELLKKREVSALEVTKSYLERIKEVEPKIDALVTITEDFALQKAKEADE 60

Query: 93  ERKRTSPSSLSPLHGIPVLVKDNIGTKDKLNTTAGSFALLGSIVPRDAGVVTKLRKAGAI 152
           + K+   ++L+   GIPV++KDNI T + + TT  S  L   I P +A VV KL + G I
Sbjct: 61  KIKKGEDTALT---GIPVIIKDNIST-EGIKTTCSSKMLENYIPPYNATVVEKLLEEGVI 120

Query: 153 IFGKASLSEWSYFRSSGLPNGWSARGGQGKNPYTLGE-PCGSSSGSAISVAANMVAVSLG 212
           I GK++L E++   S+      ++     KNP+ L   P GSS GSA ++AA+  A +LG
Sbjct: 121 ILGKSNLDEFAMGSSTE-----NSAFKTTKNPWDLSRVPGGSSGGSAAAIAADEAAFALG 180

Query: 213 TDTDGSILCPSTFNSVVGIRPTVGLTSRAGVVPISLRQDAIGPICRTVSDAAYVLDAIVG 272
           +DT GSI  P++   VVG++PT GL SR G+V  +   D IGP  + V+D A VL+ I+G
Sbjct: 181 SDTGGSIRQPASLCGVVGMKPTYGLVSRYGLVAFASSLDQIGPFTKDVTDCAIVLNTIIG 240

Query: 273 VDRYDNSTIEASKYIPKGGYGQFLRVDGLKGKRIGIVRKFFDFGHDDTFYSGAFEKVFET 332
            D  D+++++    I K  Y  +L+ D +KG RIG+ ++FF  G ++    G  E V E+
Sbjct: 241 HDPKDSTSLK----IDKPDYTSYLKED-IKGLRIGVAKEFFGEGIEE----GVKETVQES 300

Query: 333 LKQGGAILVDNLIIDNLHVIIGGSSGEWT--ALPAEFKISLNEYLKELVASPIRSLSDAI 392
           +K          ++ +L   I   S  +   ALPA + I+  E      AS   +  D I
Sbjct: 301 IK----------VLQDLGAEIIDISIPYVEYALPAYYIIASAE------ASSNLARYDGI 360

Query: 393 EFNKKNSKLEKL-------------KEYGQKLFLKAEA-TNGIGDEEKAALARVGSLSKN 452
            +     K E L             KE  +++ L   A ++G  D       +V +L KN
Sbjct: 361 RYGHIAEKYEDLIDMYMVTRSEGFGKEVKRRIMLGTYALSSGYYDAYYKKALKVRTLIKN 420

Query: 453 GLERVMIKNKLDAIAAPGWLISPVLA------------------------IGGFPGVSVP 512
             E+     K D I  P    SP +A                        I G PG+S+P
Sbjct: 421 DFEKAF--EKCDVIIGP---TSPTVAFKIGERANDPLAMYLADIYTVSVNIAGLPGISIP 480

Query: 513 AGYEPEGVPYGVGFGGLKGFEPRLIEIAYGFEQLTK-SRKPPSI 514
            G   +G+P G+   G    E +++ +AY FEQ  K S KP +I
Sbjct: 481 CGLS-DGLPVGLQIIGRHFDEGKILNVAYAFEQANKFSAKPQAI 484

BLAST of PI0015130 vs. ExPASy Swiss-Prot
Match: B0KBN4 (Glutamyl-tRNA(Gln) amidotransferase subunit A OS=Thermoanaerobacter pseudethanolicus (strain ATCC 33223 / 39E) OX=340099 GN=gatA PE=3 SV=1)

HSP 1 Score: 175.3 bits (443), Expect = 1.8e-42
Identity = 162/524 (30.92%), Postives = 257/524 (49.05%), Query Frame = 0

Query: 33  LSIEEATLKDLQLAFYKNKLTSKQLVEFYLQQVGRFNPILKGIIEVNPD-ALDQAFRADI 92
           + +   T+ +L+    K ++++ ++ + YL+++    P +  +I +  D AL +A  AD 
Sbjct: 1   MELYSLTIHELRELLKKREVSALEVTKSYLERIKEVEPKIDALITITEDFALQRAKEADE 60

Query: 93  ERKRTSPSSLSPLHGIPVLVKDNIGTKDKLNTTAGSFALLGSIVPRDAGVVTKLRKAGAI 152
           + K    ++L+   GIPV++KDNI T + + TT  S  L   I P +A VV KL + G I
Sbjct: 61  KIKNGEDTALT---GIPVIIKDNIST-EGIKTTCSSKMLENYIPPYNATVVEKLLEEGVI 120

Query: 153 IFGKASLSEWSYFRSSGLPNGWSARGGQGKNPYTLGE-PCGSSSGSAISVAANMVAVSLG 212
           I GK++L E++   S+      ++     KNP+ L   P GSS GSA ++AA+  A +LG
Sbjct: 121 ILGKSNLDEFAMGSSTE-----NSAFKTTKNPWDLSRVPGGSSGGSAAAIAADEAAFALG 180

Query: 213 TDTDGSILCPSTFNSVVGIRPTVGLTSRAGVVPISLRQDAIGPICRTVSDAAYVLDAIVG 272
           +DT GSI  P++   VVG++PT GL SR G+V  +   D IGP  + V+D A VL+ I+G
Sbjct: 181 SDTGGSIRQPASLCGVVGMKPTYGLVSRYGLVAFASSLDQIGPFTKDVTDCAIVLNTIIG 240

Query: 273 VDRYDNSTIEASKYIPKGGYGQFLRVDGLKGKRIGIVRKFFDFGHDDTFYSGAFEKVFET 332
            D  D+++++    I K  Y  +L+ D +KG RIG+ ++FF  G ++    G  E V E+
Sbjct: 241 HDPKDSTSLK----IDKPDYTSYLKED-IKGLRIGVAKEFFGEGIEE----GVKETVQES 300

Query: 333 LKQGGAILVDNLIIDNLHVIIGGSSGEWT--ALPAEFKISLNEYLKELVASPIRSLSDAI 392
           +K          ++ +L   I   S  +   ALPA + I+  E      AS   +  D I
Sbjct: 301 IK----------VLQDLGAEIIDISIPYVEYALPAYYIIASAE------ASSNLARYDGI 360

Query: 393 EFNKKNSKLEKL-------------KEYGQKLFLKAEA-TNGIGDEEKAALARVGSLSKN 452
            +     K E L             KE  +++ L   A ++G  D       +V +L KN
Sbjct: 361 RYGHIAGKYEDLIDMYMVTRSEGFGKEVKRRIMLGTYALSSGYYDAYYKKALKVRTLIKN 420

Query: 453 GLERVMIKNKLDAIAAPGWLISPVLA------------------------IGGFPGVSVP 512
             E+     K D I  P    SP +A                        I G PG+S+P
Sbjct: 421 DFEKAF--EKCDVIIGP---TSPTVAFKIGERANDPLAMYLADIYTVSVNIAGLPGISIP 480

Query: 513 AGYEPEGVPYGVGFGGLKGFEPRLIEIAYGFEQLTK-SRKPPSI 514
            G   +G+P G+   G    E +++ +AY FEQ  K + KP +I
Sbjct: 481 CGLS-DGLPVGLQIIGRHFDEGKILNVAYAFEQANKFNAKPQAI 484

BLAST of PI0015130 vs. ExPASy TrEMBL
Match: A0A0A0LLK0 (Amidase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G264040 PE=4 SV=1)

HSP 1 Score: 839.3 bits (2167), Expect = 8.4e-240
Identity = 431/514 (83.85%), Postives = 465/514 (90.47%), Query Frame = 0

Query: 6   MAAQSFHIYMFMVL----ILSSYGSCSFDTDLSIEEATLKDLQLAFYKNKLTSKQLVEFY 65
           MAAQSF IY+ M+L    ILSSYGSCSFDT+ SIEEATLKDLQLAFY+NKLTS QLVEFY
Sbjct: 1   MAAQSFPIYISMLLGLLAILSSYGSCSFDTNFSIEEATLKDLQLAFYQNKLTSTQLVEFY 60

Query: 66  LQQVGRFNPILKGIIEVNPDALDQAFRADIERKRTSPSSLSPLHGIPVLVKDNIGTKDKL 125
           L+QV RFNPIL GIIEVNPDAL+QA +AD+ERKR+SP SLSPLHGIPV VKDNI TKD+L
Sbjct: 61  LEQVRRFNPILNGIIEVNPDALNQASQADLERKRSSPRSLSPLHGIPVFVKDNIATKDQL 120

Query: 126 NTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWSYFRSSGLPNGWSARGGQGK 185
           NTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWS FRS   PNGWSARGGQGK
Sbjct: 121 NTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWSDFRSYEQPNGWSARGGQGK 180

Query: 186 NPYTLGEPCGSSSGSAISVAANMVAVSLGTDTDGSILCPSTFNSVVGIRPTVGLTSRAGV 245
           NPYT+GEPCGSSSGSAISVAANMV VSLGT+TDGSILCPST NSVVGI+PTVGLTSRAGV
Sbjct: 181 NPYTMGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGV 240

Query: 246 VPISLRQDAIGPICRTVSDAAYVLDAIVGVDRYDNSTIEASKYIPKGGYGQFLRVDGLKG 305
           VPISLRQD +GPICRTV+DAAYVLDAI G DRYDNSTIEASKY+PKGGYGQFL+ DGLKG
Sbjct: 241 VPISLRQDTVGPICRTVADAAYVLDAIAGPDRYDNSTIEASKYVPKGGYGQFLKEDGLKG 300

Query: 306 KRIGIVRKFFDFGHDDTFYSGAFEKVFETLKQGGAILVDNLIIDNLHVIIGGSSGEWTAL 365
           KRIGIVRK +DFGHDD FY GAFEKVF+TLKQGGAILVDNL I++ HVI G SSGEWTA+
Sbjct: 301 KRIGIVRKLYDFGHDDVFYIGAFEKVFKTLKQGGAILVDNLTINSFHVITGSSSGEWTAV 360

Query: 366 PAEFKISLNEYLKELVASPIRSLSDAIEFNKKNSKLEKLKEYGQKLFLKAEATNGIGDEE 425
            AEFKIS+N YLK+LVASPIRSLSDAIEFN+KNSKLEKLKEYGQ+LFL+AEAT GIG  E
Sbjct: 361 LAEFKISINVYLKQLVASPIRSLSDAIEFNRKNSKLEKLKEYGQELFLEAEATKGIGGAE 420

Query: 426 KAALARVGSLSKNGLERVMIKNKLDAIAAPGWLISPVLAIGGFPGVSVPAGYEPEGVPYG 485
           KAALAR+  LSK G ER+MIKNKLDAIAAPG LIS  LAIGGFPGVSVPAGY P+G+P+G
Sbjct: 421 KAALARLAKLSKEGFERLMIKNKLDAIAAPGRLISHFLAIGGFPGVSVPAGYNPQGLPFG 480

Query: 486 VGFGGLKGFEPRLIEIAYGFEQLTKSRKPPSIKR 516
           +GFGGLKGFEPRLIEIAYGFE LT  RK PS+ R
Sbjct: 481 IGFGGLKGFEPRLIEIAYGFEHLTMGRKSPSLGR 514

BLAST of PI0015130 vs. ExPASy TrEMBL
Match: A0A5D3BKT1 (Putative amidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold123G001390 PE=4 SV=1)

HSP 1 Score: 835.1 bits (2156), Expect = 1.6e-238
Identity = 435/512 (84.96%), Postives = 464/512 (90.62%), Query Frame = 0

Query: 6   MAAQSFHIYMFMVL----ILSSYGSCSFDTDLSIEEATLKDLQLAFYKNKLTSKQLVEFY 65
           MA QSF +Y+ M+L    ILSSYGSCSF T LSIEEATL DLQ AFY+NKLTS+QLVEFY
Sbjct: 1   MAGQSFLLYVSMLLGLMAILSSYGSCSFHTKLSIEEATLIDLQHAFYQNKLTSRQLVEFY 60

Query: 66  LQQVGRFNPILKGIIEVNPDALDQAFRADIERKRTSPSSLSPLHGIPVLVKDNIGTKDKL 125
           L+QV RFNPILKGIIEVNPDALDQA RADIERK++SP SLSPLHGIPVLVKDNI TKDKL
Sbjct: 61  LEQVRRFNPILKGIIEVNPDALDQASRADIERKKSSPRSLSPLHGIPVLVKDNIATKDKL 120

Query: 126 NTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWSYFRSSGLPNGWSARGGQGK 185
           NTTAGSFALLGSIVPRDAGVVTKLR AGAIIFGKASLSEWSYFRS+ LP+GWSARGGQGK
Sbjct: 121 NTTAGSFALLGSIVPRDAGVVTKLRMAGAIIFGKASLSEWSYFRSNELPSGWSARGGQGK 180

Query: 186 NPYTLGEPCGSSSGSAISVAANMVAVSLGTDTDGSILCPSTFNSVVGIRPTVGLTSRAGV 245
           NPY LGEPCGSSSGSAISVAANMV VSLGT+TDGSILCPST NSVVGI+PTVGLTSRAGV
Sbjct: 181 NPYILGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGV 240

Query: 246 VPISLRQDAIGPICRTVSDAAYVLDAIVGVDRYDNSTIEASKYIPKGGYGQFLRVDGLKG 305
           +PIS RQD++GPICRTVSDA YVLDAIVG DRYDNSTIEASKYIPKGGYGQFLR  GLKG
Sbjct: 241 IPISSRQDSVGPICRTVSDATYVLDAIVGADRYDNSTIEASKYIPKGGYGQFLRAGGLKG 300

Query: 306 KRIGIVRKFFDFGHDDTFYSGAFEKVFETLKQGGAILVDNLIIDNLHVIIGGSSGEWTAL 365
           KRIGIVR+F+DFGHD+TFY  A+EKV +TLK+GGAILVDN  IDNL +I  GSSGE  AL
Sbjct: 301 KRIGIVREFYDFGHDETFYPQAYEKVLKTLKKGGAILVDNRTIDNLQLIFDGSSGEQIAL 360

Query: 366 PAEFKISLNEYLKELVASPIRSLSDAIEFNKKNSKLEKLKEYGQKLFLKAEATNGIGDEE 425
            AEFKISLN YLKELVASPIRSLSDAIEFNKKNSKLE L EYGQ+LFLKAEATNGIGD E
Sbjct: 361 LAEFKISLNAYLKELVASPIRSLSDAIEFNKKNSKLENL-EYGQELFLKAEATNGIGDAE 420

Query: 426 KAALARVGSLSKNGLERVMIKNKLDAIAAPGWLISPVLAIGGFPGVSVPAGYEPEGVPYG 485
           KAALAR+  LSK+G ER+MIKNKLDAIAAPG LIS V AIGGFPGVSVPAGY+P+GVPYG
Sbjct: 421 KAALARLAKLSKDGFERLMIKNKLDAIAAPGSLISSVFAIGGFPGVSVPAGYDPQGVPYG 480

Query: 486 VGFGGLKGFEPRLIEIAYGFEQLTKSRKPPSI 514
           + FGGLKGFEPRLIEIAYGFE LTKSRKPPSI
Sbjct: 481 ITFGGLKGFEPRLIEIAYGFEHLTKSRKPPSI 511

BLAST of PI0015130 vs. ExPASy TrEMBL
Match: E5GC09 (Amidase OS=Cucumis melo subsp. melo OX=412675 PE=4 SV=1)

HSP 1 Score: 835.1 bits (2156), Expect = 1.6e-238
Identity = 435/512 (84.96%), Postives = 464/512 (90.62%), Query Frame = 0

Query: 6   MAAQSFHIYMFMVL----ILSSYGSCSFDTDLSIEEATLKDLQLAFYKNKLTSKQLVEFY 65
           MA QSF +Y+ M+L    ILSSYGSCSF T LSIEEATL DLQ AFY+NKLTS+QLVEFY
Sbjct: 1   MAGQSFLLYVSMLLGLMAILSSYGSCSFHTKLSIEEATLIDLQHAFYQNKLTSRQLVEFY 60

Query: 66  LQQVGRFNPILKGIIEVNPDALDQAFRADIERKRTSPSSLSPLHGIPVLVKDNIGTKDKL 125
           L+QV RFNPILKGIIEVNPDALDQA RADIERK++SP SLSPLHGIPVLVKDNI TKDKL
Sbjct: 61  LEQVRRFNPILKGIIEVNPDALDQASRADIERKKSSPRSLSPLHGIPVLVKDNIATKDKL 120

Query: 126 NTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWSYFRSSGLPNGWSARGGQGK 185
           NTTAGSFALLGSIVPRDAGVVTKLR AGAIIFGKASLSEWSYFRS+ LP+GWSARGGQGK
Sbjct: 121 NTTAGSFALLGSIVPRDAGVVTKLRMAGAIIFGKASLSEWSYFRSNELPSGWSARGGQGK 180

Query: 186 NPYTLGEPCGSSSGSAISVAANMVAVSLGTDTDGSILCPSTFNSVVGIRPTVGLTSRAGV 245
           NPY LGEPCGSSSGSAISVAANMV VSLGT+TDGSILCPST NSVVGI+PTVGLTSRAGV
Sbjct: 181 NPYILGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGV 240

Query: 246 VPISLRQDAIGPICRTVSDAAYVLDAIVGVDRYDNSTIEASKYIPKGGYGQFLRVDGLKG 305
           +PIS RQD++GPICRTVSDA YVLDAIVG DRYDNSTIEASKYIPKGGYGQFLR  GLKG
Sbjct: 241 IPISSRQDSVGPICRTVSDATYVLDAIVGADRYDNSTIEASKYIPKGGYGQFLRAGGLKG 300

Query: 306 KRIGIVRKFFDFGHDDTFYSGAFEKVFETLKQGGAILVDNLIIDNLHVIIGGSSGEWTAL 365
           KRIGIVR+F+DFGHD+TFY  A+EKV +TLK+GGAILVDN  IDNL +I  GSSGE  AL
Sbjct: 301 KRIGIVREFYDFGHDETFYPQAYEKVLKTLKKGGAILVDNRTIDNLQLIFDGSSGEQIAL 360

Query: 366 PAEFKISLNEYLKELVASPIRSLSDAIEFNKKNSKLEKLKEYGQKLFLKAEATNGIGDEE 425
            AEFKISLN YLKELVASPIRSLSDAIEFNKKNSKLE L EYGQ+LFLKAEATNGIGD E
Sbjct: 361 LAEFKISLNAYLKELVASPIRSLSDAIEFNKKNSKLENL-EYGQELFLKAEATNGIGDAE 420

Query: 426 KAALARVGSLSKNGLERVMIKNKLDAIAAPGWLISPVLAIGGFPGVSVPAGYEPEGVPYG 485
           KAALAR+  LSK+G ER+MIKNKLDAIAAPG LIS V AIGGFPGVSVPAGY+P+GVPYG
Sbjct: 421 KAALARLAKLSKDGFERLMIKNKLDAIAAPGSLISSVFAIGGFPGVSVPAGYDPQGVPYG 480

Query: 486 VGFGGLKGFEPRLIEIAYGFEQLTKSRKPPSI 514
           + FGGLKGFEPRLIEIAYGFE LTKSRKPPSI
Sbjct: 481 ITFGGLKGFEPRLIEIAYGFEHLTKSRKPPSI 511

BLAST of PI0015130 vs. ExPASy TrEMBL
Match: A0A1S3C5K8 (putative amidase C869.01 OS=Cucumis melo OX=3656 GN=LOC103497307 PE=4 SV=1)

HSP 1 Score: 835.1 bits (2156), Expect = 1.6e-238
Identity = 435/512 (84.96%), Postives = 464/512 (90.62%), Query Frame = 0

Query: 6   MAAQSFHIYMFMVL----ILSSYGSCSFDTDLSIEEATLKDLQLAFYKNKLTSKQLVEFY 65
           MA QSF +Y+ M+L    ILSSYGSCSF T LSIEEATL DLQ AFY+NKLTS+QLVEFY
Sbjct: 1   MAGQSFLLYVSMLLGLMAILSSYGSCSFHTKLSIEEATLIDLQHAFYQNKLTSRQLVEFY 60

Query: 66  LQQVGRFNPILKGIIEVNPDALDQAFRADIERKRTSPSSLSPLHGIPVLVKDNIGTKDKL 125
           L+QV RFNPILKGIIEVNPDALDQA RADIERK++SP SLSPLHGIPVLVKDNI TKDKL
Sbjct: 61  LEQVRRFNPILKGIIEVNPDALDQASRADIERKKSSPRSLSPLHGIPVLVKDNIATKDKL 120

Query: 126 NTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWSYFRSSGLPNGWSARGGQGK 185
           NTTAGSFALLGSIVPRDAGVVTKLR AGAIIFGKASLSEWSYFRS+ LP+GWSARGGQGK
Sbjct: 121 NTTAGSFALLGSIVPRDAGVVTKLRMAGAIIFGKASLSEWSYFRSNELPSGWSARGGQGK 180

Query: 186 NPYTLGEPCGSSSGSAISVAANMVAVSLGTDTDGSILCPSTFNSVVGIRPTVGLTSRAGV 245
           NPY LGEPCGSSSGSAISVAANMV VSLGT+TDGSILCPST NSVVGI+PTVGLTSRAGV
Sbjct: 181 NPYILGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGV 240

Query: 246 VPISLRQDAIGPICRTVSDAAYVLDAIVGVDRYDNSTIEASKYIPKGGYGQFLRVDGLKG 305
           +PIS RQD++GPICRTVSDA YVLDAIVG DRYDNSTIEASKYIPKGGYGQFLR  GLKG
Sbjct: 241 IPISSRQDSVGPICRTVSDATYVLDAIVGADRYDNSTIEASKYIPKGGYGQFLRAGGLKG 300

Query: 306 KRIGIVRKFFDFGHDDTFYSGAFEKVFETLKQGGAILVDNLIIDNLHVIIGGSSGEWTAL 365
           KRIGIVR+F+DFGHD+TFY  A+EKV +TLK+GGAILVDN  IDNL +I  GSSGE  AL
Sbjct: 301 KRIGIVREFYDFGHDETFYPQAYEKVLKTLKKGGAILVDNRTIDNLQLIFDGSSGEQIAL 360

Query: 366 PAEFKISLNEYLKELVASPIRSLSDAIEFNKKNSKLEKLKEYGQKLFLKAEATNGIGDEE 425
            AEFKISLN YLKELVASPIRSLSDAIEFNKKNSKLE L EYGQ+LFLKAEATNGIGD E
Sbjct: 361 LAEFKISLNAYLKELVASPIRSLSDAIEFNKKNSKLENL-EYGQELFLKAEATNGIGDAE 420

Query: 426 KAALARVGSLSKNGLERVMIKNKLDAIAAPGWLISPVLAIGGFPGVSVPAGYEPEGVPYG 485
           KAALAR+  LSK+G ER+MIKNKLDAIAAPG LIS V AIGGFPGVSVPAGY+P+GVPYG
Sbjct: 421 KAALARLAKLSKDGFERLMIKNKLDAIAAPGSLISSVFAIGGFPGVSVPAGYDPQGVPYG 480

Query: 486 VGFGGLKGFEPRLIEIAYGFEQLTKSRKPPSI 514
           + FGGLKGFEPRLIEIAYGFE LTKSRKPPSI
Sbjct: 481 ITFGGLKGFEPRLIEIAYGFEHLTKSRKPPSI 511

BLAST of PI0015130 vs. ExPASy TrEMBL
Match: A0A0A0LM97 (Amidase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G264020 PE=4 SV=1)

HSP 1 Score: 831.6 bits (2147), Expect = 1.8e-237
Identity = 432/515 (83.88%), Postives = 468/515 (90.87%), Query Frame = 0

Query: 6   MAAQSFHIYMFMVL----ILSSYGSCSFDTDLSIEEATLKDLQLAFYKNKLTSKQLVEFY 65
           MA QSF + + M+L    ILSS GSCSF T+LS+EEATLKDLQ AFY+NKLTS+QLVEFY
Sbjct: 1   MAGQSFLLDISMLLGLMAILSSCGSCSFHTELSLEEATLKDLQRAFYQNKLTSRQLVEFY 60

Query: 66  LQQVGRFNPILKGIIEVNPDALDQAFRADIERKRTSPSSLSPLHGIPVLVKDNIGTKDKL 125
           L+QV R NPILKGIIEVNPDALDQA RAD+ERK++SP SLSPLHGIPVLVKDNI TKDKL
Sbjct: 61  LEQVRRLNPILKGIIEVNPDALDQASRADLERKKSSPRSLSPLHGIPVLVKDNIATKDKL 120

Query: 126 NTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWSYFRSSGLPNGWSARGGQGK 185
           NTTAGSFALLGS+VPRDAGVVTKLR AGAIIFGKASLSEWSYFRS+ LP+GWSARGGQGK
Sbjct: 121 NTTAGSFALLGSVVPRDAGVVTKLRMAGAIIFGKASLSEWSYFRSNALPSGWSARGGQGK 180

Query: 186 NPYTLGEPCGSSSGSAISVAANMVAVSLGTDTDGSILCPSTFNSVVGIRPTVGLTSRAGV 245
           NPYT+GEPCGSSSGSAISVAANMV VSLGT+TDGSILCPST NSVVGI+PTVGLTSRAGV
Sbjct: 181 NPYTMGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGV 240

Query: 246 VPISLRQDAIGPICRTVSDAAYVLDAIVGVDRYDNSTIEASKYIPKGGYGQFLRVDGLKG 305
           VPIS RQD +GPICRTVSDAAYVL+AIVG DRYDNSTIEASKYIPKGGYGQFLR  GLKG
Sbjct: 241 VPISSRQDTVGPICRTVSDAAYVLEAIVGADRYDNSTIEASKYIPKGGYGQFLRAGGLKG 300

Query: 306 KRIGIVRKFFDFGHDDTFYSGAFEKVFETLKQGGAILVDNLIIDNLHVIIGGSSGEWTAL 365
           KRIGIVR+F+DFG DDTFY+ A+EKV +TLK+GGAILVDNL+IDNL  I  GSSGE  AL
Sbjct: 301 KRIGIVREFYDFGPDDTFYTQAYEKVVKTLKKGGAILVDNLMIDNLEQIFDGSSGEQIAL 360

Query: 366 PAEFKISLNEYLKELVASPIRSLSDAIEFNKKNSKLEKLKEYGQKLFLKAEATNGIGDEE 425
            AEFKISLN YLKELVASPIRSLSDAIEFNKKNSKLE L EYGQ+ FLKAEATNGIGD E
Sbjct: 361 LAEFKISLNAYLKELVASPIRSLSDAIEFNKKNSKLENL-EYGQEEFLKAEATNGIGDAE 420

Query: 426 KAALARVGSLSKNGLERVMIKNKLDAIAAPGWLISPVLAIGGFPGVSVPAGYEPEGVPYG 485
           KAALAR+  LSK+G ER+MIKNKLDA+AAPG LISPV AIGGFPGVSVPAGY+P+G PYG
Sbjct: 421 KAALARLAKLSKDGFERLMIKNKLDAVAAPGSLISPVFAIGGFPGVSVPAGYDPQGNPYG 480

Query: 486 VGFGGLKGFEPRLIEIAYGFEQLTKSRKPPSIKRR 517
           + FGGLKGFEPRLIEIAYGFE+LTKSRKPPSIKR+
Sbjct: 481 ICFGGLKGFEPRLIEIAYGFERLTKSRKPPSIKRQ 514

BLAST of PI0015130 vs. NCBI nr
Match: XP_038900913.1 (probable amidase At4g34880 [Benincasa hispida])

HSP 1 Score: 868.2 bits (2242), Expect = 3.5e-248
Identity = 451/514 (87.74%), Postives = 476/514 (92.61%), Query Frame = 0

Query: 6   MAAQSFHIYMFMVL----ILSSYGSCSFDTDLSIEEATLKDLQLAFYKNKLTSKQLVEFY 65
           MAAQSF +Y+ MVL    ILSSYG+CSF T   IEEAT+KDLQLAFY+NKLTS+QLV+FY
Sbjct: 1   MAAQSFWLYISMVLSLVAILSSYGNCSFGTHFPIEEATVKDLQLAFYQNKLTSRQLVKFY 60

Query: 66  LQQVGRFNPILKGIIEVNPDALDQAFRADIERKRTSPSSLSPLHGIPVLVKDNIGTKDKL 125
           L+QV RFNPILKGIIEVNPDALDQA RAD ERKR+S SSLSPLHGIPVL+KDNI TKDKL
Sbjct: 61  LKQVRRFNPILKGIIEVNPDALDQATRADHERKRSSLSSLSPLHGIPVLLKDNIATKDKL 120

Query: 126 NTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWSYFRSSGLPNGWSARGGQGK 185
           NTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWS FRS+ LPNGW ARGGQGK
Sbjct: 121 NTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWSQFRSN-LPNGWCARGGQGK 180

Query: 186 NPYTLGEPCGSSSGSAISVAANMVAVSLGTDTDGSILCPSTFNSVVGIRPTVGLTSRAGV 245
           NPYT+GEPCGSSSGSAISVAANMVAVSLGTDTDGSILCPST+NSVVGIRPTVGLTSRAGV
Sbjct: 181 NPYTMGEPCGSSSGSAISVAANMVAVSLGTDTDGSILCPSTYNSVVGIRPTVGLTSRAGV 240

Query: 246 VPISLRQDAIGPICRTVSDAAYVLDAIVGVDRYDNSTIEASKYIPKGGYGQFLRVDGLKG 305
           VPIS RQDA+GPICRTVSDAAYVLDAIVG D+YDNSTIEASKYIPKGGYGQFLR DGLKG
Sbjct: 241 VPISSRQDAVGPICRTVSDAAYVLDAIVGADKYDNSTIEASKYIPKGGYGQFLRPDGLKG 300

Query: 306 KRIGIVRKFFDFGHDDTFYSGAFEKVFETLKQGGAILVDNLIIDNLHVIIGGSSGEWTAL 365
            RIGIVRKFFDFG D  FYS A+EKVF++LKQGGAILVDNL ID+L +IIGG SGEWTAL
Sbjct: 301 TRIGIVRKFFDFGDDGVFYSEAYEKVFKSLKQGGAILVDNLTIDSLDIIIGGLSGEWTAL 360

Query: 366 PAEFKISLNEYLKELVASPIRSLSDAIEFNKKNSKLEKLKEYGQKLFLKAEATNGIGDEE 425
           PAEFKIS+N YLKELVASPIRSLSDAIEFNKKNSKLEKLKEYGQ LFL+AEATNGIGD E
Sbjct: 361 PAEFKISINAYLKELVASPIRSLSDAIEFNKKNSKLEKLKEYGQVLFLEAEATNGIGDVE 420

Query: 426 KAALARVGSLSKNGLERVMIKNKLDAIAAPGWLISPVLAIGGFPGVSVPAGYEPEGVPYG 485
           KAALA V SLSKNG ERVMIKNKLDAIAAPG LISPVLAIGGFPG+SVPAGY P+GVPYG
Sbjct: 421 KAALASVASLSKNGFERVMIKNKLDAIAAPGGLISPVLAIGGFPGISVPAGYSPDGVPYG 480

Query: 486 VGFGGLKGFEPRLIEIAYGFEQLTKSRKPPSIKR 516
           +GFGGLKGFEPRLIEIAYGFEQLTKSRKPP IKR
Sbjct: 481 IGFGGLKGFEPRLIEIAYGFEQLTKSRKPPLIKR 513

BLAST of PI0015130 vs. NCBI nr
Match: XP_038900912.1 (LOW QUALITY PROTEIN: probable amidase At4g34880 [Benincasa hispida])

HSP 1 Score: 842.4 bits (2175), Expect = 2.0e-240
Identity = 431/520 (82.88%), Postives = 470/520 (90.38%), Query Frame = 0

Query: 1   MSRAAMAAQSFHIYMFMVL----ILSSYGSCSFDTDLSIEEATLKDLQLAFYKNKLTSKQ 60
           M RAAMAAQSF +Y+ M+L    ILSSYGSCSFDT LSIEEAT+KDLQLAFY+NKL S+Q
Sbjct: 1   MCRAAMAAQSFWLYISMLLSLLAILSSYGSCSFDTHLSIEEATVKDLQLAFYQNKLISRQ 60

Query: 61  LVEFYLQQVGRFNPILKGIIEVNPDALDQAFRADIERKRTSPSSLSPLHGIPVLVKDNIG 120
           LV+FYL  V RFNPILKGIIEVNPDALDQA +AD+ERKR SPSSLSPLHGIPVLVKDNI 
Sbjct: 61  LVKFYLNXVRRFNPILKGIIEVNPDALDQASQADLERKRNSPSSLSPLHGIPVLVKDNIA 120

Query: 121 TKDKLNTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWSYFRSSGLPNGWSAR 180
           T+DKLNTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLS+WSYFRS  +P+GWSAR
Sbjct: 121 TRDKLNTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSKWSYFRSYXIPSGWSAR 180

Query: 181 GGQGKNPYTLGEPCGSSSGSAISVAANMVAVSLGTDTDGSILCPSTFNSVVGIRPTVGLT 240
           GGQGKNPYT+GEPCGSS GSAIS+AANMVAVSLGT+TDGSILCPS  NSVVGI+PTVGLT
Sbjct: 181 GGQGKNPYTMGEPCGSSGGSAISIAANMVAVSLGTETDGSILCPSILNSVVGIKPTVGLT 240

Query: 241 SRAGVVPISLRQDAIGPICRTVSDAAYVLDAIVGVDRYDNSTIEASKYIPKGGYGQFLRV 300
           SRAGV+PISLRQD +GPICRTVSD AYVL AIVG D+ DNSTIEASKYIP+GGY QFLR 
Sbjct: 241 SRAGVIPISLRQDTVGPICRTVSDDAYVLGAIVGADKNDNSTIEASKYIPRGGYAQFLRA 300

Query: 301 DGLKGKRIGIVRKFFDFGHDDTFYSGAFEKVFETLKQGGAILVDNLIIDNLHVIIGGSSG 360
           DGLKGKRIGIVRKFFDFGHDD FY  A+EKVF+TL+QGGA+ VDNL ID+L VI+G SSG
Sbjct: 301 DGLKGKRIGIVRKFFDFGHDDAFYPRAYEKVFKTLRQGGAVFVDNLTIDSLQVIMGSSSG 360

Query: 361 EWTALPAEFKISLNEYLKELVASPIRSLSDAIEFNKKNSKLEKLKEYGQKLFLKAEATNG 420
           E TAL AEFKISLN YLK+LVASPIRSLSDAIEFN+KNSKLEKL+EYGQ LF++AEAT G
Sbjct: 361 EETALLAEFKISLNAYLKQLVASPIRSLSDAIEFNEKNSKLEKLREYGQNLFIEAEATKG 420

Query: 421 IGDEEKAALARVGSLSKNGLERVMIKNKLDAIAAPGWLISPVLAIGGFPGVSVPAGYEPE 480
           IGD EKAALAR+  LSK+G ERVM+KNKLDAIAAPGW IS VLAIGGFPG+SVPAGY  +
Sbjct: 421 IGDAEKAALARLAKLSKDGFERVMLKNKLDAIAAPGWEISSVLAIGGFPGISVPAGYNSQ 480

Query: 481 GVPYGVGFGGLKGFEPRLIEIAYGFEQLTKSRKPPSIKRR 517
           GVP+G+ FGGLKGFEPRLIEIAYGFE LTKSRK PSIKRR
Sbjct: 481 GVPFGISFGGLKGFEPRLIEIAYGFEHLTKSRKSPSIKRR 520

BLAST of PI0015130 vs. NCBI nr
Match: XP_004147024.3 (probable amidase At4g34880 [Cucumis sativus])

HSP 1 Score: 840.9 bits (2171), Expect = 6.0e-240
Identity = 432/514 (84.05%), Postives = 466/514 (90.66%), Query Frame = 0

Query: 6   MAAQSFHIYMFMVL----ILSSYGSCSFDTDLSIEEATLKDLQLAFYKNKLTSKQLVEFY 65
           MAAQSF IY+ M+L    ILSSYGSCSFDT+ SIEEATLKDLQLAFY+NKLTS QLVEFY
Sbjct: 1   MAAQSFPIYISMLLGLLAILSSYGSCSFDTNFSIEEATLKDLQLAFYQNKLTSTQLVEFY 60

Query: 66  LQQVGRFNPILKGIIEVNPDALDQAFRADIERKRTSPSSLSPLHGIPVLVKDNIGTKDKL 125
           L+QV RFNPIL GIIEVNPDAL+QA +AD+ERKR+SP SLSPLHGIPVLVKDNI TKD+L
Sbjct: 61  LEQVRRFNPILNGIIEVNPDALNQASQADLERKRSSPRSLSPLHGIPVLVKDNIATKDQL 120

Query: 126 NTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWSYFRSSGLPNGWSARGGQGK 185
           NTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWS FRS   PNGWSARGGQGK
Sbjct: 121 NTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWSDFRSYEQPNGWSARGGQGK 180

Query: 186 NPYTLGEPCGSSSGSAISVAANMVAVSLGTDTDGSILCPSTFNSVVGIRPTVGLTSRAGV 245
           NPYT+GEPCGSSSGSAISVAANMV VSLGT+TDGSILCPST NSVVGI+PTVGLTSRAGV
Sbjct: 181 NPYTMGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGV 240

Query: 246 VPISLRQDAIGPICRTVSDAAYVLDAIVGVDRYDNSTIEASKYIPKGGYGQFLRVDGLKG 305
           VPISLRQD +GPICRTV+DAAYVLDAI G DRYDNSTIEASKY+PKGGYGQFL+ DGLKG
Sbjct: 241 VPISLRQDTVGPICRTVADAAYVLDAIAGPDRYDNSTIEASKYVPKGGYGQFLKEDGLKG 300

Query: 306 KRIGIVRKFFDFGHDDTFYSGAFEKVFETLKQGGAILVDNLIIDNLHVIIGGSSGEWTAL 365
           KRIGIVRK +DFGHDD FY GAFEKVF+TLKQGGAILVDNL I++ HVI G SSGEWTA+
Sbjct: 301 KRIGIVRKLYDFGHDDVFYIGAFEKVFKTLKQGGAILVDNLTINSFHVITGSSSGEWTAV 360

Query: 366 PAEFKISLNEYLKELVASPIRSLSDAIEFNKKNSKLEKLKEYGQKLFLKAEATNGIGDEE 425
            AEFKIS+N YLK+LVASPIRSLSDAIEFN+KNSKLEKLKEYGQ+LFL+AEAT GIG  E
Sbjct: 361 LAEFKISINVYLKQLVASPIRSLSDAIEFNRKNSKLEKLKEYGQELFLEAEATKGIGGAE 420

Query: 426 KAALARVGSLSKNGLERVMIKNKLDAIAAPGWLISPVLAIGGFPGVSVPAGYEPEGVPYG 485
           KAALAR+  LSK G ER+MIKNKLDAIAAPG LIS  LAIGGFPGVSVPAGY P+G+P+G
Sbjct: 421 KAALARLAKLSKEGFERLMIKNKLDAIAAPGRLISHFLAIGGFPGVSVPAGYNPQGLPFG 480

Query: 486 VGFGGLKGFEPRLIEIAYGFEQLTKSRKPPSIKR 516
           +GFGGLKGFEPRLIEIAYGFE LT  RK PS+ R
Sbjct: 481 IGFGGLKGFEPRLIEIAYGFEHLTMGRKSPSLGR 514

BLAST of PI0015130 vs. NCBI nr
Match: XP_008457661.1 (PREDICTED: putative amidase C869.01 [Cucumis melo] >ADN34008.1 amidase [Cucumis melo subsp. melo] >KAA0045711.1 putative amidase [Cucumis melo var. makuwa] >TYJ99569.1 putative amidase [Cucumis melo var. makuwa])

HSP 1 Score: 835.1 bits (2156), Expect = 3.3e-238
Identity = 435/512 (84.96%), Postives = 464/512 (90.62%), Query Frame = 0

Query: 6   MAAQSFHIYMFMVL----ILSSYGSCSFDTDLSIEEATLKDLQLAFYKNKLTSKQLVEFY 65
           MA QSF +Y+ M+L    ILSSYGSCSF T LSIEEATL DLQ AFY+NKLTS+QLVEFY
Sbjct: 1   MAGQSFLLYVSMLLGLMAILSSYGSCSFHTKLSIEEATLIDLQHAFYQNKLTSRQLVEFY 60

Query: 66  LQQVGRFNPILKGIIEVNPDALDQAFRADIERKRTSPSSLSPLHGIPVLVKDNIGTKDKL 125
           L+QV RFNPILKGIIEVNPDALDQA RADIERK++SP SLSPLHGIPVLVKDNI TKDKL
Sbjct: 61  LEQVRRFNPILKGIIEVNPDALDQASRADIERKKSSPRSLSPLHGIPVLVKDNIATKDKL 120

Query: 126 NTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWSYFRSSGLPNGWSARGGQGK 185
           NTTAGSFALLGSIVPRDAGVVTKLR AGAIIFGKASLSEWSYFRS+ LP+GWSARGGQGK
Sbjct: 121 NTTAGSFALLGSIVPRDAGVVTKLRMAGAIIFGKASLSEWSYFRSNELPSGWSARGGQGK 180

Query: 186 NPYTLGEPCGSSSGSAISVAANMVAVSLGTDTDGSILCPSTFNSVVGIRPTVGLTSRAGV 245
           NPY LGEPCGSSSGSAISVAANMV VSLGT+TDGSILCPST NSVVGI+PTVGLTSRAGV
Sbjct: 181 NPYILGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGV 240

Query: 246 VPISLRQDAIGPICRTVSDAAYVLDAIVGVDRYDNSTIEASKYIPKGGYGQFLRVDGLKG 305
           +PIS RQD++GPICRTVSDA YVLDAIVG DRYDNSTIEASKYIPKGGYGQFLR  GLKG
Sbjct: 241 IPISSRQDSVGPICRTVSDATYVLDAIVGADRYDNSTIEASKYIPKGGYGQFLRAGGLKG 300

Query: 306 KRIGIVRKFFDFGHDDTFYSGAFEKVFETLKQGGAILVDNLIIDNLHVIIGGSSGEWTAL 365
           KRIGIVR+F+DFGHD+TFY  A+EKV +TLK+GGAILVDN  IDNL +I  GSSGE  AL
Sbjct: 301 KRIGIVREFYDFGHDETFYPQAYEKVLKTLKKGGAILVDNRTIDNLQLIFDGSSGEQIAL 360

Query: 366 PAEFKISLNEYLKELVASPIRSLSDAIEFNKKNSKLEKLKEYGQKLFLKAEATNGIGDEE 425
            AEFKISLN YLKELVASPIRSLSDAIEFNKKNSKLE L EYGQ+LFLKAEATNGIGD E
Sbjct: 361 LAEFKISLNAYLKELVASPIRSLSDAIEFNKKNSKLENL-EYGQELFLKAEATNGIGDAE 420

Query: 426 KAALARVGSLSKNGLERVMIKNKLDAIAAPGWLISPVLAIGGFPGVSVPAGYEPEGVPYG 485
           KAALAR+  LSK+G ER+MIKNKLDAIAAPG LIS V AIGGFPGVSVPAGY+P+GVPYG
Sbjct: 421 KAALARLAKLSKDGFERLMIKNKLDAIAAPGSLISSVFAIGGFPGVSVPAGYDPQGVPYG 480

Query: 486 VGFGGLKGFEPRLIEIAYGFEQLTKSRKPPSI 514
           + FGGLKGFEPRLIEIAYGFE LTKSRKPPSI
Sbjct: 481 ITFGGLKGFEPRLIEIAYGFEHLTKSRKPPSI 511

BLAST of PI0015130 vs. NCBI nr
Match: XP_004147023.1 (probable amidase At4g34880 [Cucumis sativus] >KGN61907.1 hypothetical protein Csa_006678 [Cucumis sativus])

HSP 1 Score: 831.6 bits (2147), Expect = 3.6e-237
Identity = 432/515 (83.88%), Postives = 468/515 (90.87%), Query Frame = 0

Query: 6   MAAQSFHIYMFMVL----ILSSYGSCSFDTDLSIEEATLKDLQLAFYKNKLTSKQLVEFY 65
           MA QSF + + M+L    ILSS GSCSF T+LS+EEATLKDLQ AFY+NKLTS+QLVEFY
Sbjct: 1   MAGQSFLLDISMLLGLMAILSSCGSCSFHTELSLEEATLKDLQRAFYQNKLTSRQLVEFY 60

Query: 66  LQQVGRFNPILKGIIEVNPDALDQAFRADIERKRTSPSSLSPLHGIPVLVKDNIGTKDKL 125
           L+QV R NPILKGIIEVNPDALDQA RAD+ERK++SP SLSPLHGIPVLVKDNI TKDKL
Sbjct: 61  LEQVRRLNPILKGIIEVNPDALDQASRADLERKKSSPRSLSPLHGIPVLVKDNIATKDKL 120

Query: 126 NTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWSYFRSSGLPNGWSARGGQGK 185
           NTTAGSFALLGS+VPRDAGVVTKLR AGAIIFGKASLSEWSYFRS+ LP+GWSARGGQGK
Sbjct: 121 NTTAGSFALLGSVVPRDAGVVTKLRMAGAIIFGKASLSEWSYFRSNALPSGWSARGGQGK 180

Query: 186 NPYTLGEPCGSSSGSAISVAANMVAVSLGTDTDGSILCPSTFNSVVGIRPTVGLTSRAGV 245
           NPYT+GEPCGSSSGSAISVAANMV VSLGT+TDGSILCPST NSVVGI+PTVGLTSRAGV
Sbjct: 181 NPYTMGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGV 240

Query: 246 VPISLRQDAIGPICRTVSDAAYVLDAIVGVDRYDNSTIEASKYIPKGGYGQFLRVDGLKG 305
           VPIS RQD +GPICRTVSDAAYVL+AIVG DRYDNSTIEASKYIPKGGYGQFLR  GLKG
Sbjct: 241 VPISSRQDTVGPICRTVSDAAYVLEAIVGADRYDNSTIEASKYIPKGGYGQFLRAGGLKG 300

Query: 306 KRIGIVRKFFDFGHDDTFYSGAFEKVFETLKQGGAILVDNLIIDNLHVIIGGSSGEWTAL 365
           KRIGIVR+F+DFG DDTFY+ A+EKV +TLK+GGAILVDNL+IDNL  I  GSSGE  AL
Sbjct: 301 KRIGIVREFYDFGPDDTFYTQAYEKVVKTLKKGGAILVDNLMIDNLEQIFDGSSGEQIAL 360

Query: 366 PAEFKISLNEYLKELVASPIRSLSDAIEFNKKNSKLEKLKEYGQKLFLKAEATNGIGDEE 425
            AEFKISLN YLKELVASPIRSLSDAIEFNKKNSKLE L EYGQ+ FLKAEATNGIGD E
Sbjct: 361 LAEFKISLNAYLKELVASPIRSLSDAIEFNKKNSKLENL-EYGQEEFLKAEATNGIGDAE 420

Query: 426 KAALARVGSLSKNGLERVMIKNKLDAIAAPGWLISPVLAIGGFPGVSVPAGYEPEGVPYG 485
           KAALAR+  LSK+G ER+MIKNKLDA+AAPG LISPV AIGGFPGVSVPAGY+P+G PYG
Sbjct: 421 KAALARLAKLSKDGFERLMIKNKLDAVAAPGSLISPVFAIGGFPGVSVPAGYDPQGNPYG 480

Query: 486 VGFGGLKGFEPRLIEIAYGFEQLTKSRKPPSIKRR 517
           + FGGLKGFEPRLIEIAYGFE+LTKSRKPPSIKR+
Sbjct: 481 ICFGGLKGFEPRLIEIAYGFERLTKSRKPPSIKRQ 514

BLAST of PI0015130 vs. TAIR 10
Match: AT4G34880.1 (Amidase family protein )

HSP 1 Score: 492.3 bits (1266), Expect = 4.8e-139
Identity = 273/510 (53.53%), Postives = 357/510 (70.00%), Query Frame = 0

Query: 11  FHIYMFMVLILS-----SYGSCS---FDTDLSIEEATLKDLQLAFYKNKLTSKQLVEFYL 70
           F   + ++LILS     S GS S     +  SI+EAT++D+++AF + +LTSKQLVE YL
Sbjct: 8   FFSSLLILLILSQALIMSVGSASQIRLSSTFSIQEATIEDIRVAFNEKRLTSKQLVELYL 67

Query: 71  QQVGRFNPILKGIIEVNPDALDQAFRADIERKRTSPSSLSPLHGIPVLVKDNIGTKDKLN 130
           + + + NPIL  +IE NPDAL QA  AD ER   + + L  LHG+PVL+KD+I TKDKLN
Sbjct: 68  EAISKLNPILHAVIETNPDALIQAEIADRERDLKNTTKLPILHGVPVLLKDSISTKDKLN 127

Query: 131 TTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWSYFRSSGLPNGWSARGGQGKN 190
           TTAGSFALLGS+V RDAGVV +LR++GA+I GKASLSEW++FRS  +P+GWSA       
Sbjct: 128 TTAGSFALLGSVVARDAGVVKRLRESGAVILGKASLSEWAHFRSFSIPDGWSA------- 187

Query: 191 PYTLGEPCGSSSGSAISVAANMVAVSLGTDTDGSILCPSTFNSVVGIRPTVGLTSRAGVV 250
                                                PS  NSVVGI+P+VGLTSRAGVV
Sbjct: 188 -------------------------------------PSQ-NSVVGIKPSVGLTSRAGVV 247

Query: 251 PISLRQDAIGPICRTVSDAAYVLDAIVGVDRYDNSTIEASKYIPKGGYGQFLRVDGLKGK 310
           PISLRQD+IGPICRTVSDA ++LDAIVG D  D +T  AS++IP+GGY QFL   GLKGK
Sbjct: 248 PISLRQDSIGPICRTVSDAVHLLDAIVGYDPLDEATKTASEFIPEGGYKQFLTTSGLKGK 307

Query: 311 RIGIVRKFFDFGHDDTFYSGAFEKVFETLKQGGAILVDNLIIDNLHVIIGGS-SGEWTAL 370
           R+GIV K          +S   +   +TL++ GAI+++NL I N+ VI+GG+ SGE  AL
Sbjct: 308 RLGIVMK----------HSSLLDHHIKTLRREGAIVINNLTIPNIEVIVGGTDSGEEIAL 367

Query: 371 PAEFKISLNEYLKELVASPIRSLSDAIEFNKKNSKLEKLKEYGQKLFLKAEATNGIGDEE 430
            AEFK+SLN YLKELV SP+RSL+D I +N++ ++ EK+KE+GQ++FL AEAT+G+G++E
Sbjct: 368 LAEFKMSLNAYLKELVKSPVRSLADVIAYNEEFAEQEKVKEWGQEVFLTAEATSGMGEKE 427

Query: 431 KAALARVGSLSKNGLERVMIKNKLDAIAAPGWLISPVLAIGGFPGVSVPAGYEPEGVPYG 490
           K AL ++  LS+NG+E+++ +NKLDAI   G  +S VLAIGG+PG++VPAGY+  GVPYG
Sbjct: 428 KTALQKMKELSRNGIEKLIEENKLDAIVTLGSDLSSVLAIGGYPGINVPAGYDSGGVPYG 462

Query: 491 VGFGGLKGFEPRLIEIAYGFEQLTKSRKPP 512
           + FGGL+  EP+LIEIA+ FEQ T  RKPP
Sbjct: 488 ISFGGLRFSEPKLIEIAFAFEQATLIRKPP 462

BLAST of PI0015130 vs. TAIR 10
Match: AT3G25660.1 (Amidase family protein )

HSP 1 Score: 134.4 bits (337), Expect = 2.6e-31
Identity = 144/513 (28.07%), Postives = 236/513 (46.00%), Query Frame = 0

Query: 20  ILSSYGSCSFDTD-LSIEEATLKDLQLAFYKNKLTSKQLVEFYLQQVGRFNPILKGIIEV 79
           I+S+  S + DT  +S  ++ +   + +    + T+ ++ + YL ++    P LK  + V
Sbjct: 25  IVSAASSKTIDTSVISPPQSQILTTRRSLLSGETTAVEIAKSYLSRIRLTEPQLKCFLHV 84

Query: 80  NPDALDQAFRADIERKRTSPSSLSPLHGIPVLVKDNIGTKDKLNTTAGSFALLGSIVPRD 139
           + + L  A   +I+++      L PL G+ + VKDNI T+  + +TA S  L     P D
Sbjct: 85  SENVLKDA--QEIDQRIAKGEELGPLAGVLIGVKDNICTQG-MPSTAASRILEHYRPPFD 144

Query: 140 AGVVTKLRKAGAIIFGKASLSEWSYFRSSGLPNGWSARGGQ-GKNPYTLGE-PCGSSSGS 199
           A  V K+++ G I+ GK ++ E+      G+ +   A   Q   NP+ L   P GSS GS
Sbjct: 145 ATAVKKIKELGGIVVGKTNMDEF------GMGSTTEASAFQVTANPWDLSRVPGGSSGGS 204

Query: 200 AISVAANMVAVSLGTDTDGSILCPSTFNSVVGIRPTVGLTSRAGVVPISLRQDAIGPICR 259
           A +VAA    VSLG+DT GS+  P++F  VVG++PT G  SR G++  +   D IG    
Sbjct: 205 AAAVAARQCMVSLGSDTGGSVRQPASFCGVVGLKPTYGRVSRFGLMAYASSLDVIGCFGS 264

Query: 260 TVSDAAYVLDAIVGVDRYDNSTIEASKYIPKGGYGQFLRVD-----GLKGKRIGIVRKFF 319
           TV+DA  +L AI G DR+D++   +SK        QFL VD      L G ++GI+R+  
Sbjct: 265 TVADAGMLLHAISGYDRFDST---SSKQDVPEFQSQFLSVDHFESKPLNGVKVGIIRETL 324

Query: 320 DFGHDDTFYSGAFEKVFETLKQGGAILVD------NLIIDNLHVIIGGSS----GEWTAL 379
           + G D    S A ++    L+  G IL +      +L +   +VI    S      +  +
Sbjct: 325 EDGVDSGVRS-ATQEAASHLEALGCILTEVSLPSFSLGLPAYYVIASSESSSNLSRYDGV 384

Query: 380 PAEFKISLNEYLKELVASPIRSLSDAIEFNKKNSKLEKLKEYGQKLFLKAEATNG-IGDE 439
               ++   E  K    S        ++             Y    + +A+     I  +
Sbjct: 385 RYGNQVMAEELNKLYECSRGEGFGGEVKMRILMGTYALSAGYYDAYYKRAQQVRTLIRKD 444

Query: 440 EKAALARVGSLSKNGLERVMIK---NKLDAIAA-PGWLISPVLAIGGFPGVSVPAGY--- 499
            KAAL +   L          K    K D +A   G +++  + + G P + +P G    
Sbjct: 445 FKAALEQNDILISPAAPSAAYKIGEKKDDPLAMYAGDIMTVNVNLAGLPAMVLPCGLVEG 504

Query: 500 EPEGVPYGVGFGGLKGFEPRLIEIAYGFEQLTK 507
            P G+P G+   G    E +L+++ + FEQ  K
Sbjct: 505 GPSGLPVGLQMIGAAFDEEKLLKVGHIFEQTLK 524

BLAST of PI0015130 vs. TAIR 10
Match: AT5G07360.1 (Amidase family protein )

HSP 1 Score: 121.7 bits (304), Expect = 1.7e-27
Identity = 82/232 (35.34%), Postives = 126/232 (54.31%), Query Frame = 0

Query: 51  KLTSKQLVEFYLQQVGRFNPILKGIIEVNPD-ALDQAFRADIERKRTSPSSLSPLHGIPV 110
           ++TSK+LV  YL+Q+ R+N +L+ ++    + A  QA  AD     +  + L PLHGIP 
Sbjct: 216 QITSKELVRIYLKQLKRYNHVLEAVVTYTEELAYKQAKEAD--DLLSQGTYLGPLHGIPY 275

Query: 111 LVKDNIGTKDKLNTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWSYFRSSGL 170
            +KD +       TT GS +     +  +A V  +L+ +GA++  K      +Y      
Sbjct: 276 GLKDIVAVPG-YKTTWGSTSFKDQFLDIEAWVYKRLKASGAVLVAKLVTGSMAY------ 335

Query: 171 PNGWSARGGQGKNPYTLGE-PCGSSSGSAISVAANMVAVSLGTDTDGSILCPSTFNSVVG 230
            + W   GG+ +NP+ + E   GSS+G A S +A MV  ++G++T GS+  P+    +  
Sbjct: 336 DDIWF--GGRTRNPWNIEEFSTGSSAGPAASTSAGMVPFAIGSETAGSMTYPAARCGITA 395

Query: 231 IRPTVGLTSRAGVVPISLRQDAIGPICRTVSDAAYVLDAIVGVDRYDNSTIE 281
           +RPT G   R GV+ IS   D +GP CRT +D A +LDAI G D  D S+ E
Sbjct: 396 LRPTFGSVGRTGVMSISESLDKLGPFCRTAADCAVILDAIKGKDPDDLSSRE 436

BLAST of PI0015130 vs. TAIR 10
Match: AT5G07360.2 (Amidase family protein )

HSP 1 Score: 110.9 bits (276), Expect = 3.0e-24
Identity = 80/232 (34.48%), Postives = 122/232 (52.59%), Query Frame = 0

Query: 51  KLTSKQLVEFYLQQVGRFNPILKGIIEVNPD-ALDQAFRADIERKRTSPSSLSPLHGIPV 110
           ++TSK+LV  YL+Q+ R+N +L+ ++    + A  QA  AD     +  + L PLHGIP 
Sbjct: 216 QITSKELVRIYLKQLKRYNHVLEAVVTYTEELAYKQAKEAD--DLLSQGTYLGPLHGIPY 275

Query: 111 LVKDNIGTKDKLNTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWSYFRSSGL 170
            +KD +       TT GS +     +  +A V  +L+ +GA++  K      +Y      
Sbjct: 276 GLKDIVAVPG-YKTTWGSTSFKDQFLDIEAWVYKRLKASGAVLVAKLVTGSMAY------ 335

Query: 171 PNGWSARGGQGKNPYTLGE-PCGSSSGSAISVAANMVAVSLGTDTDGSILCPSTFNSVVG 230
            + W   GG+ +NP+ + E   GSS+G A S +A       G++T GS+  P+    +  
Sbjct: 336 DDIWF--GGRTRNPWNIEEFSTGSSAGPAASTSA-------GSETAGSMTYPAARCGITA 395

Query: 231 IRPTVGLTSRAGVVPISLRQDAIGPICRTVSDAAYVLDAIVGVDRYDNSTIE 281
           +RPT G   R GV+ IS   D +GP CRT +D A +LDAI G D  D S+ E
Sbjct: 396 LRPTFGSVGRTGVMSISESLDKLGPFCRTAADCAVILDAIKGKDPDDLSSRE 429

BLAST of PI0015130 vs. TAIR 10
Match: AT5G64440.1 (fatty acid amide hydrolase )

HSP 1 Score: 109.4 bits (272), Expect = 8.8e-24
Identity = 127/485 (26.19%), Postives = 201/485 (41.44%), Query Frame = 0

Query: 48  YKNKLT-----SKQLVEFYLQQVGRFNPILKGIIEVNPDALDQAFRADIERKRTSPSSLS 107
           Y++KLT     +K+++   +++ G   P    +I  + + + +   A   R+    + +S
Sbjct: 138 YRSKLTTPLQVAKRIISI-IEEFGYDKPPTPFLIRFDANEVIKQAEAS-TRRFEQGNPIS 197

Query: 108 PLHGIPVLVKDNIGTKDKLNTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWS 167
            L GI V +KD+I           ++      V +D+ VV+KLR  GAI+ GKA++ E  
Sbjct: 198 VLDGIFVTIKDDIDCLPHPTNGGTTWLHEDRSVEKDSAVVSKLRSCGAILLGKANMHELG 257

Query: 168 YFRSSGLPNGWSARGGQGKNPYTLGEPCGSSSGSAISVAANMVAVSLGTDTDGSILCPST 227
              +    N  + R       YT     GSSSGSA  VAA + + +LGTD  GS+  PS 
Sbjct: 258 MGTTGNNSNYGTTRNPHDPKRYT----GGSSSGSAAIVAAGLCSAALGTDGGGSVRIPSA 317

Query: 228 FNSVVGIRPTVGLTSRAGVVPISLRQDAIGPICRTVSDAAYVLDAIVGVDRYDNSTIEAS 287
              + G++ T G T   G +      + IGP+  ++ DA  V  AI+G    D   ++ S
Sbjct: 318 LCGITGLKTTYGRTDMTGSLCEGGTVEIIGPLASSLEDAFLVYAAILGSSSADRYNLKPS 377

Query: 288 -----KYIPKGGYGQFLRVDGLKGKRIGIVRKFF-DFGHDDTFYSGAFEKVFETLKQGGA 347
                K +   G       + +   R+G   K+F D    D   S   E + + L     
Sbjct: 378 PPCFPKLLSHNG------SNAIGSLRLGKYTKWFNDVSSSD--ISDKCEDILKLLSNNHG 437

Query: 348 ILVDNLIIDNL------HVIIGGSS--GEWTALPAEFKISLNEYLKELVASPIRSLSDAI 407
             V  +++  L      HVI  GS      T      K S   Y      +  RS S A 
Sbjct: 438 CKVVEIVVPELEEMRAAHVISIGSPTLSSLTPYCEAGKNSKLSYDTRTSFAIFRSFS-AS 497

Query: 408 EFNKKNSKLEKLKEYGQKLFLKAEATNGIGDEEKAALARVGSLSKNGLERVMIKNKLDAI 467
           ++        +L EY   +F   +          A +    +L KNG   + +   L   
Sbjct: 498 DYIAAQCLRRRLMEYHLNIFKDVDVIVTPTTGMTAPVIPPDAL-KNGETNIQVTTDLMRF 557

Query: 468 AAPGWLISPVLAIGGFPGVSVPAGYEPEGVPYGVGFGGLKGFEPRLIEIAYGFEQLTKSR 514
                L+       GFP +SVP GY+ EG+P G+   G    E  ++ +A   E+L    
Sbjct: 558 VLAANLL-------GFPAISVPVGYDKEGLPIGLQIMGRPWAEATVLGLAAAVEELAPVT 599

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
A0A1P8B7604.0e-16260.08Probable amidase At4g34880 OS=Arabidopsis thaliana OX=3702 GN=At4g34880 PE=2 SV=... [more]
Q9URY42.3e-7738.13Putative amidase C869.01 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) ... [more]
D4B3C83.5e-6535.08Putative amidase ARB_02965 OS=Arthroderma benhamiae (strain ATCC MYA-4681 / CBS ... [more]
B0K3S36.3e-4330.92Glutamyl-tRNA(Gln) amidotransferase subunit A OS=Thermoanaerobacter sp. (strain ... [more]
B0KBN41.8e-4230.92Glutamyl-tRNA(Gln) amidotransferase subunit A OS=Thermoanaerobacter pseudethanol... [more]
Match NameE-valueIdentityDescription
A0A0A0LLK08.4e-24083.85Amidase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G264040 PE=... [more]
A0A5D3BKT11.6e-23884.96Putative amidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold123G001... [more]
E5GC091.6e-23884.96Amidase OS=Cucumis melo subsp. melo OX=412675 PE=4 SV=1[more]
A0A1S3C5K81.6e-23884.96putative amidase C869.01 OS=Cucumis melo OX=3656 GN=LOC103497307 PE=4 SV=1[more]
A0A0A0LM971.8e-23783.88Amidase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G264020 PE=... [more]
Match NameE-valueIdentityDescription
XP_038900913.13.5e-24887.74probable amidase At4g34880 [Benincasa hispida][more]
XP_038900912.12.0e-24082.88LOW QUALITY PROTEIN: probable amidase At4g34880 [Benincasa hispida][more]
XP_004147024.36.0e-24084.05probable amidase At4g34880 [Cucumis sativus][more]
XP_008457661.13.3e-23884.96PREDICTED: putative amidase C869.01 [Cucumis melo] >ADN34008.1 amidase [Cucumis ... [more]
XP_004147023.13.6e-23783.88probable amidase At4g34880 [Cucumis sativus] >KGN61907.1 hypothetical protein Cs... [more]
Match NameE-valueIdentityDescription
AT4G34880.14.8e-13953.53Amidase family protein [more]
AT3G25660.12.6e-3128.07Amidase family protein [more]
AT5G07360.11.7e-2735.34Amidase family protein [more]
AT5G07360.23.0e-2434.48Amidase family protein [more]
AT5G64440.18.8e-2426.19fatty acid amide hydrolase [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036928Amidase signature (AS) superfamilyGENE3D3.90.1300.10Amidase signature (AS) domaincoord: 25..513
e-value: 4.1E-141
score: 473.4
IPR036928Amidase signature (AS) superfamilySUPERFAMILY75304Amidase signature (AS) enzymescoord: 34..512
IPR023631Amidase signature domainPFAMPF01425Amidasecoord: 57..402
e-value: 4.6E-67
score: 227.0
NoneNo IPR availablePANTHERPTHR42678:SF19C869.01-LIKE PROTEIN, PUTATIVE-RELATEDcoord: 17..514
NoneNo IPR availablePANTHERPTHR42678AMIDASEcoord: 17..514

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0015130.1PI0015130.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0050566 asparaginyl-tRNA synthase (glutamine-hydrolyzing) activity