PI0009160 (gene) Melon (PI 482460) v1

Overview
NamePI0009160
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
DescriptionRING-type E3 ubiquitin transferase
Locationchr04: 17212007 .. 17213488 (+)
RNA-Seq ExpressionPI0009160
SyntenyPI0009160
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGAGATCCTGTCATTCTCTCTACTGGCGAGGTTTGTCATCATTTCTTTTCTTTTTTTTTTTTTTTTTTCCTTCTCTTCTAGGTCTTCAAATTAATCTAATTCGCGCTTCTTGCTCTTTGTTCTTGACAAGATTTTTAGCTTTTTGGATTACGATTTTTTATTTAACTTGTATGTTTATTGACATTGCATCTTTGTATTTTGTTTTTTTAATTTAAATGAATTCAAACCCTTTGGATTGGATCTTCTGTTAAATTGTATAGAATTGAGTAGGGGGACGAGACGTTCTTGAGGATCTTTTGTTATATTATATGTATGGTTTTCGTTTTGGTTTGGGGGGGACTGGATATGATCTCTTGCCCATTATATTATATCGTTTGATTTCCTTTCATTATGTGGTTTTTGAGTTTCTTCTTCTTATTTGCAGACATATGACAGACCTTCATTCAGAAATGGTTAAATTCAGGGAACAGGACTTGCCCTAGAACTCAGCAAGTTCTTTCGCCACACCAATCTCACTCCCAATCACTTGATTCGAGAAATGATATCTCAGTGGTGTGCAACTCGGGGAATGAGTTGCAAGATCGTGTCCGTGTTCATATCTTGATGACGATGTCATAACAGAGGCTGATCGAGACCGTTTTCTCATGTACTTGAGAAAATGTCTATGACAATATCTGAACAGAAAGAAGCTGCAAAGGAGCTAAGAATGTTGACGAAGCGAATGCCTTCATTTCGAGCACTTTTGGTGAGTCCCTTGATGCCATATCTTTGTTGCTTAACCACTTTGGGAGATAAGTCACAAAGTAGTAGTAGTGTTCACACGGATTTGCAAGAAGATGTGATTCAACCCTCTTGAATTTGTCGATCCATGACAACAACAAGAAACTTGTTGCCGAAACACCAATGCAATACCTCTTCTAATGGAAGCATTGAGATCAGGAACCATGGAAACAAGGAGCAAGGCTGCAGCCCTATTTACTCTATCTGCTCTCGACCCAAACAAAGCAATCATTGGGAAAGCTGGTGCCTTGAAACCACTTATGAGCTTTTGGACTTGGGGCATCCATATCAATCAAAGATGCGCTTCAGCTATTCAATCTTGTATACTTCATGAAAACAAAGCAAGAGCCGTGCGTGATGGTGCAGTTCGAGTGCTTCTGAAAAAGATTATGAGCCAGATGCATGTCGATGAGTTGTTGGCTATTTTGCAATGCTATCATGCCATCAAGAGCTATTGAAGAAATTGGAGAACTGGAGCTGTCCCCTCTTACTCAGAATATCAGGGAAAGTTCTTGTTCACGTAACAAGGAAAATTGCATTGTGATCATCCATTCTGTCTGCTTATACGATCGAACCAAATGGAAGGATATGAGGGAAGAAGAGAAATGCTATCGAACCATTTCTGAACTTGCTCAGAATGGGACTTCAAGGGCCAAGAGAAAAGCCAGTGGCATACTTGAAAGAATAAACGGTAGTTAA

mRNA sequence

ATGAGAGATCCTGTCATTCTCTCTACTGGCGAGACCTTCATTCAGAAATGGTTAAATTCAGGGAACAGGACTTGCCCTAGAACTCAGCAAGTTCTTTCGCCACACCAATCTCACTCCCAATCACTTGATTCGAGAAATGATATCTCAGTGGTGTGCAACTCGGGGAATGAGTTGCAAGATCGTGTCCGTGTTCATATCTTGATGACGATAAGCTGCAAAGGAGCTAAGAATGTTGACGAAGCGAATGCCTTCATTTCGAGCACTTTTGGTGAGTCCCTTGATGCCATATCTTTATGCATGTCGATGAGTTGTTGGCTATTTTGCAATGCTATCATGCCATCAAGAGCTATTGAAGAAATTGGAGAACTGGAGCTGTCCCCTCTTACTCAGAATATCAGGGAAAGTTCTTGTTCACGTAACAAGGAAAATTGCATTGTGATCATCCATTCTGTCTGCTTATACGATCGAACCAAATGGAAGGATATGAGGGAAGAAGAGAAATGCTATCGAACCATTTCTGAACTTGCTCAGAATGGGACTTCAAGGGCCAAGAGAAAAGCCAGTGGCATACTTGAAAGAATAAACGGTAGTTAA

Coding sequence (CDS)

ATGAGAGATCCTGTCATTCTCTCTACTGGCGAGACCTTCATTCAGAAATGGTTAAATTCAGGGAACAGGACTTGCCCTAGAACTCAGCAAGTTCTTTCGCCACACCAATCTCACTCCCAATCACTTGATTCGAGAAATGATATCTCAGTGGTGTGCAACTCGGGGAATGAGTTGCAAGATCGTGTCCGTGTTCATATCTTGATGACGATAAGCTGCAAAGGAGCTAAGAATGTTGACGAAGCGAATGCCTTCATTTCGAGCACTTTTGGTGAGTCCCTTGATGCCATATCTTTATGCATGTCGATGAGTTGTTGGCTATTTTGCAATGCTATCATGCCATCAAGAGCTATTGAAGAAATTGGAGAACTGGAGCTGTCCCCTCTTACTCAGAATATCAGGGAAAGTTCTTGTTCACGTAACAAGGAAAATTGCATTGTGATCATCCATTCTGTCTGCTTATACGATCGAACCAAATGGAAGGATATGAGGGAAGAAGAGAAATGCTATCGAACCATTTCTGAACTTGCTCAGAATGGGACTTCAAGGGCCAAGAGAAAAGCCAGTGGCATACTTGAAAGAATAAACGGTAGTTAA

Protein sequence

MRDPVILSTGETFIQKWLNSGNRTCPRTQQVLSPHQSHSQSLDSRNDISVVCNSGNELQDRVRVHILMTISCKGAKNVDEANAFISSTFGESLDAISLCMSMSCWLFCNAIMPSRAIEEIGELELSPLTQNIRESSCSRNKENCIVIIHSVCLYDRTKWKDMREEEKCYRTISELAQNGTSRAKRKASGILERINGS
Homology
BLAST of PI0009160 vs. ExPASy Swiss-Prot
Match: Q9SRT0 (U-box domain-containing protein 9 OS=Arabidopsis thaliana OX=3702 GN=PUB9 PE=1 SV=1)

HSP 1 Score: 97.8 bits (242), Expect = 1.4e-19
Identity = 46/81 (56.79%), Postives = 66/81 (81.48%), Query Frame = 0

Query: 115 RAIEEIGEL-ELSPLTQNIRESSCSRNKENCIVIIHSVCLYDRTKWKDMREEEKCYRTIS 174
           +A+EE+GEL  +S L +  RES C RNKEN IVI+H++C  DRTKWK+++EEE  + TI+
Sbjct: 370 KAVEELGELGGVSWLLKITRESECKRNKENAIVILHTICFSDRTKWKEIKEEENAHGTIT 429

Query: 175 ELAQNGTSRAKRKASGILERI 195
           +L++ GTSRA+RKA+GIL+R+
Sbjct: 430 KLSREGTSRAQRKANGILDRL 450


HSP 2 Score: 63.9 bits (154), Expect = 2.3e-09
Identity = 36/69 (52.17%), Postives = 48/69 (69.57%), Query Frame = 0

Query: 1   MRDPVILSTGET----FIQKWLNSGNRTCPRTQQVLSPHQSHSQSLDSRNDISVVCNSGN 60
           MRDPV+L++G+T    FIQKWL+SGNRTCP+TQQVL PH + + +L  R  IS  C   N
Sbjct: 87  MRDPVVLASGQTYDKLFIQKWLSSGNRTCPKTQQVL-PHTALTPNLLIREMISKWCKK-N 146

Query: 61  ELQDRVRVH 66
            L+ + + H
Sbjct: 147 GLETKSQYH 153

BLAST of PI0009160 vs. ExPASy Swiss-Prot
Match: Q5VRH9 (U-box domain-containing protein 12 OS=Oryza sativa subsp. japonica OX=39947 GN=PUB12 PE=2 SV=1)

HSP 1 Score: 53.1 bits (126), Expect = 4.0e-06
Identity = 55/200 (27.50%), Postives = 94/200 (47.00%), Query Frame = 0

Query: 1   MRDPVILSTGETF----IQKWLNSGNRTCPRTQQVLSPHQSHSQSLDSRNDISVVCN-SG 60
           M+DPVI+S+G+T+    IQKWL+SG++TCP+TQQ LS H S + +   ++ IS  C  +G
Sbjct: 241 MQDPVIVSSGQTYERSCIQKWLDSGHKTCPKTQQPLS-HTSLTPNFVLKSLISQWCEANG 300

Query: 61  NELQDRVRVHILMTISCKGAKNVDEANAFISSTFGE----SLDAISLCMSMSCWLFCNAI 120
            EL    +     +   K AK+ D  +A + S        + D           L    +
Sbjct: 301 IELPKNKQ----NSRDKKAAKSSDYDHAGLVSLMNRLRSGNQDEQRAAAGEIRLLAKRNV 360

Query: 121 MPSRAIEEIGELELSPLTQNIRESSCSRNKENCIVIIHSVCLYDRTKWKDMREEEKCYRT 180
                I E G +   PL  N+  SS  R +E+ +  + ++ +++  K   +  +      
Sbjct: 361 NNRICIAEAGAI---PLLVNLLSSSDPRTQEHAVTALLNLSIHENNKASIV--DSHAIPK 420

Query: 181 ISELAQNGTSRAKRKASGIL 192
           I E+ + G+   +  A+  L
Sbjct: 421 IVEVLKTGSMETRENAAATL 430

BLAST of PI0009160 vs. ExPASy Swiss-Prot
Match: Q9SNC6 (U-box domain-containing protein 13 OS=Arabidopsis thaliana OX=3702 GN=PUB13 PE=1 SV=1)

HSP 1 Score: 48.5 bits (114), Expect = 9.9e-05
Identity = 20/37 (54.05%), Postives = 29/37 (78.38%), Query Frame = 0

Query: 1   MRDPVILSTGETF----IQKWLNSGNRTCPRTQQVLS 34
           MRDPVI+S+G+T+    I+KW+  G+ TCP+TQQ L+
Sbjct: 269 MRDPVIVSSGQTYERTCIEKWIEGGHSTCPKTQQALT 305

BLAST of PI0009160 vs. ExPASy Swiss-Prot
Match: Q8VZ40 (U-box domain-containing protein 14 OS=Arabidopsis thaliana OX=3702 GN=PUB14 PE=1 SV=1)

HSP 1 Score: 48.1 bits (113), Expect = 1.3e-04
Identity = 46/198 (23.23%), Postives = 92/198 (46.46%), Query Frame = 0

Query: 1   MRDPVILSTGETF----IQKWLNSGNRTCPRTQQVLSPHQSHSQSLDSRNDISVVCNSGN 60
           M+DPVI+STG+T+    IQKWL++G++TCP++Q+ L  H   + +   ++ I++ C S N
Sbjct: 261 MKDPVIVSTGQTYERSSIQKWLDAGHKTCPKSQETLL-HAGLTPNYVLKSLIALWCES-N 320

Query: 61  ELQDRVRVHILMTISCKGAKNVDEANAFISSTFGESLDAISLCMSMSCWLFCNAIMPSRA 120
            ++         T    G+ + D    F+ S   +  +  +     +       ++  R 
Sbjct: 321 GIELPQNQGSCRTTKIGGSSSSDCDRTFVLSLLEKLANGTTEQQRAAAGEL--RLLAKRN 380

Query: 121 IEE---IGELELSPLTQNIRESSCSRNKENCIVIIHSVCLYDRTKWKDMREEEKCYRTIS 180
           ++    I E    PL   +  S   R +E+ +  + ++ + +  K   +  +      I 
Sbjct: 381 VDNRVCIAEAGAIPLLVELLSSPDPRTQEHSVTALLNLSINEGNKGAIV--DAGAITDIV 440

Query: 181 ELAQNGTSRAKRKASGIL 192
           E+ +NG+  A+  A+  L
Sbjct: 441 EVLKNGSMEARENAAATL 452

BLAST of PI0009160 vs. ExPASy Swiss-Prot
Match: Q681N2 (U-box domain-containing protein 15 OS=Arabidopsis thaliana OX=3702 GN=PUB15 PE=2 SV=2)

HSP 1 Score: 48.1 bits (113), Expect = 1.3e-04
Identity = 25/60 (41.67%), Postives = 38/60 (63.33%), Query Frame = 0

Query: 1   MRDPVILSTGETF----IQKWLNSGNRTCPRTQQVLSPHQSHSQSLDSRNDISVVCNSGN 57
           M DPVI++TG+T+    IQKW ++G++TCP+T+Q L  H S + +   +N I   C   N
Sbjct: 303 MLDPVIIATGQTYEKESIQKWFDAGHKTCPKTRQELD-HLSLAPNFALKNLIMQWCEKNN 361

BLAST of PI0009160 vs. ExPASy TrEMBL
Match: A0A0A0L8U1 (RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_3G634320 PE=4 SV=1)

HSP 1 Score: 167.9 bits (424), Expect = 4.2e-38
Identity = 143/368 (38.86%), Postives = 155/368 (42.12%), Query Frame = 0

Query: 1   MRDPVILSTGET----FIQKWLNSGNRTCPRTQQVLSPHQSHSQSLDSRNDISVVC-NSG 60
           MRDPVILSTGET    FIQKWLNSGNRTCPRTQQVLS H + + +   R  IS  C   G
Sbjct: 86  MRDPVILSTGETYDRPFIQKWLNSGNRTCPRTQQVLS-HTNLTPNHLIREMISQWCATRG 145

Query: 61  NELQDRVRVHIL---------------------MTIS-----CKGAKNVDEANAFISSTF 120
            ELQDRVRVH L                     +T+S      K  + + +      + F
Sbjct: 146 IELQDRVRVHYLDDDVITEADRDRFLMLLEKMSLTVSEQKEAAKELRMLTKRMPSFRALF 205

Query: 121 GESLDAISLCMSMSCW-------------------------------------------- 180
           GESLDAISL +S  C                                             
Sbjct: 206 GESLDAISLLLSPLCGDKSQSSSSIHTDLQEDVITTLLNLSIHDNNKKLVAETPYAIPLL 265

Query: 181 ------------------LF--------------CNAIMP-------------------- 196
                             LF                A+ P                    
Sbjct: 266 MEALRSGTMETRSNAAAALFTLSALDPNKTIIGKAGALKPLIELLDLGHPLSIKDAASAI 325

BLAST of PI0009160 vs. ExPASy TrEMBL
Match: A0A5A7VQS8 (RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold238G00370 PE=4 SV=1)

HSP 1 Score: 164.9 bits (416), Expect = 3.5e-37
Identity = 142/368 (38.59%), Postives = 154/368 (41.85%), Query Frame = 0

Query: 1   MRDPVILSTGET----FIQKWLNSGNRTCPRTQQVLSPHQSHSQSLDSRNDISVVC-NSG 60
           MRDPVILSTGET    FIQKWLNSGNRTCPRTQQVLS H   + +   R  IS  C   G
Sbjct: 86  MRDPVILSTGETYDRPFIQKWLNSGNRTCPRTQQVLS-HTDLTPNHLIREMISQWCATRG 145

Query: 61  NELQDRVRVHIL---------------------MTIS-----CKGAKNVDEANAFISSTF 120
            ELQDRVRVH L                     MTIS      K  + + +      + F
Sbjct: 146 IELQDRVRVHYLDDDVITEADRDRFLMLLEKMSMTISEQKEAAKELRMLTKRMPSFRALF 205

Query: 121 GESLDAISLCMS------------------------------------------------ 180
           GESLDAISL +S                                                
Sbjct: 206 GESLDAISLLLSPLSGDKSQSNSNIHTDLQEDLITTLLNLSIHDNNKKLVAETPYAIPLL 265

Query: 181 --------------MSCWLF--------------CNAIMP-------------------- 196
                          +  LF                A+ P                    
Sbjct: 266 MEALRSGTMETRSNAAAALFTLSALDPNKAIIGKAGALKPLIELLDLGHPLSIKDAASAI 325

BLAST of PI0009160 vs. ExPASy TrEMBL
Match: A0A5D3DRP9 (RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1185G00250 PE=4 SV=1)

HSP 1 Score: 164.9 bits (416), Expect = 3.5e-37
Identity = 142/368 (38.59%), Postives = 154/368 (41.85%), Query Frame = 0

Query: 1   MRDPVILSTGET----FIQKWLNSGNRTCPRTQQVLSPHQSHSQSLDSRNDISVVC-NSG 60
           MRDPVILSTGET    FIQKWLNSGNRTCPRTQQVLS H   + +   R  IS  C   G
Sbjct: 86  MRDPVILSTGETYDRPFIQKWLNSGNRTCPRTQQVLS-HTDLTPNHLIREMISQWCATRG 145

Query: 61  NELQDRVRVHIL---------------------MTIS-----CKGAKNVDEANAFISSTF 120
            ELQDRVRVH L                     MTIS      K  + + +      + F
Sbjct: 146 IELQDRVRVHYLDDDVITEADRDRFLMLLEKMSMTISEQKEAAKELRMLTKRMPSFRALF 205

Query: 121 GESLDAISLCMS------------------------------------------------ 180
           GESLDAISL +S                                                
Sbjct: 206 GESLDAISLLLSPLSGDKSQSNSNIHTDLQEDVITTLLNLSIHDNNKKLVAETPYAIPLL 265

Query: 181 --------------MSCWLF--------------CNAIMP-------------------- 196
                          +  LF                A+ P                    
Sbjct: 266 MEALRSGTMETRSNAAAALFTLSALDPNKAIIGKAGALKPLIELLDLGHPLSIKDAASAI 325

BLAST of PI0009160 vs. ExPASy TrEMBL
Match: A0A1S3CDM9 (RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103499224 PE=4 SV=1)

HSP 1 Score: 164.9 bits (416), Expect = 3.5e-37
Identity = 142/368 (38.59%), Postives = 154/368 (41.85%), Query Frame = 0

Query: 1   MRDPVILSTGET----FIQKWLNSGNRTCPRTQQVLSPHQSHSQSLDSRNDISVVC-NSG 60
           MRDPVILSTGET    FIQKWLNSGNRTCPRTQQVLS H   + +   R  IS  C   G
Sbjct: 86  MRDPVILSTGETYDRPFIQKWLNSGNRTCPRTQQVLS-HTDLTPNHLIREMISQWCATRG 145

Query: 61  NELQDRVRVHIL---------------------MTIS-----CKGAKNVDEANAFISSTF 120
            ELQDRVRVH L                     MTIS      K  + + +      + F
Sbjct: 146 IELQDRVRVHYLDDDVITEADRDRFLMLLEKMSMTISEQKEAAKELRMLTKRMPSFRALF 205

Query: 121 GESLDAISLCMS------------------------------------------------ 180
           GESLDAISL +S                                                
Sbjct: 206 GESLDAISLLLSPLSGDKSQSNSNIHTDLQEDVITTLLNLSIHDNNKKLVAETPYAIPLL 265

Query: 181 --------------MSCWLF--------------CNAIMP-------------------- 196
                          +  LF                A+ P                    
Sbjct: 266 MEALRSGTMETRSNAAAALFTLSALDPNKAIIGKAGALKPLIELLDLGHPLSIKDAASAI 325

BLAST of PI0009160 vs. ExPASy TrEMBL
Match: A0A6J1HKK5 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111463870 PE=4 SV=1)

HSP 1 Score: 155.6 bits (392), Expect = 2.1e-34
Identity = 133/366 (36.34%), Postives = 153/366 (41.80%), Query Frame = 0

Query: 1   MRDPVILSTGET----FIQKWLNSGNRTCPRTQQVLSPHQSHSQSLDSRNDISVVCNS-G 60
           MRDPV++STGET    FI KWL SGNRTCPRTQQVLS H + + +   R  IS  C S G
Sbjct: 85  MRDPVVVSTGETYDRPFIHKWLRSGNRTCPRTQQVLS-HTNLTPNHLIREMISQWCTSRG 144

Query: 61  NELQDRVRVHIL---------------------MTI-----SCKGAKNVDEANAFISSTF 120
            ELQDRVRVH L                     MTI     + K  + + +      + F
Sbjct: 145 IELQDRVRVHCLDDDVITEADRDRFLTLIENMSMTIPEQKAAAKELRMLTKRMPSFRALF 204

Query: 121 GESLDAISLCMSMSCW-------------------------------------------- 180
           GESLDAISL +S  C                                             
Sbjct: 205 GESLDAISLLLSPLCGDKSQSSVHADLQEDVITTLLNLSIHDNNKKLVAETPNVIPLLKE 264

Query: 181 ----------------LFCNAIMPS----------------------------------- 196
                           LF  + + S                                   
Sbjct: 265 ALRSGTMETRSNAAAALFTLSALDSNKALIGKSGALKPLIDLLDEGHPLSMKDAASAIFN 324

BLAST of PI0009160 vs. NCBI nr
Match: XP_004144394.1 (U-box domain-containing protein 9 [Cucumis sativus] >KGN58380.1 hypothetical protein Csa_002394 [Cucumis sativus])

HSP 1 Score: 167.9 bits (424), Expect = 8.6e-38
Identity = 143/368 (38.86%), Postives = 155/368 (42.12%), Query Frame = 0

Query: 1   MRDPVILSTGET----FIQKWLNSGNRTCPRTQQVLSPHQSHSQSLDSRNDISVVC-NSG 60
           MRDPVILSTGET    FIQKWLNSGNRTCPRTQQVLS H + + +   R  IS  C   G
Sbjct: 86  MRDPVILSTGETYDRPFIQKWLNSGNRTCPRTQQVLS-HTNLTPNHLIREMISQWCATRG 145

Query: 61  NELQDRVRVHIL---------------------MTIS-----CKGAKNVDEANAFISSTF 120
            ELQDRVRVH L                     +T+S      K  + + +      + F
Sbjct: 146 IELQDRVRVHYLDDDVITEADRDRFLMLLEKMSLTVSEQKEAAKELRMLTKRMPSFRALF 205

Query: 121 GESLDAISLCMSMSCW-------------------------------------------- 180
           GESLDAISL +S  C                                             
Sbjct: 206 GESLDAISLLLSPLCGDKSQSSSSIHTDLQEDVITTLLNLSIHDNNKKLVAETPYAIPLL 265

Query: 181 ------------------LF--------------CNAIMP-------------------- 196
                             LF                A+ P                    
Sbjct: 266 MEALRSGTMETRSNAAAALFTLSALDPNKTIIGKAGALKPLIELLDLGHPLSIKDAASAI 325

BLAST of PI0009160 vs. NCBI nr
Match: XP_038888241.1 (U-box domain-containing protein 9 [Benincasa hispida])

HSP 1 Score: 164.9 bits (416), Expect = 7.3e-37
Identity = 140/367 (38.15%), Postives = 155/367 (42.23%), Query Frame = 0

Query: 1   MRDPVILSTGET----FIQKWLNSGNRTCPRTQQVLSPHQSHSQSLDSRNDISVVCNS-G 60
           MRDPVI+STGET    FIQKWL SGNRTCPRTQQVLS H + + +   R  IS  C S G
Sbjct: 86  MRDPVIVSTGETYDKPFIQKWLKSGNRTCPRTQQVLS-HTNLTPNHLIREMISQWCTSRG 145

Query: 61  NELQDRVRVHIL---------------------MTIS-----CKGAKNVDEANAFISSTF 120
            ELQDRVRVH L                     MTIS      K  + + +      + F
Sbjct: 146 IELQDRVRVHYLDDDVITEADRDRFLTLIEKMSMTISEQKAAAKELRMLTKRMPSFRALF 205

Query: 121 GESLDAISLCMSMSCW-------------------------------------------- 180
           GESLDAISL +S  C                                             
Sbjct: 206 GESLDAISLLLSPLCGDKSQSSSIHTDLQEDVITTLLNLSIHDNNKKLVIETPNVIPLLM 265

Query: 181 -----------------LF--------------CNAIMP--------------------- 196
                            LF                A+ P                     
Sbjct: 266 EALGSGTMETRSNAAAALFTLSALDSNKAIIGKAGALKPLIKLLDEGHPLSIKDAASAIF 325

BLAST of PI0009160 vs. NCBI nr
Match: XP_008460394.1 (PREDICTED: U-box domain-containing protein 9 [Cucumis melo] >TYK26069.1 U-box domain-containing protein 9 [Cucumis melo var. makuwa])

HSP 1 Score: 164.9 bits (416), Expect = 7.3e-37
Identity = 142/368 (38.59%), Postives = 154/368 (41.85%), Query Frame = 0

Query: 1   MRDPVILSTGET----FIQKWLNSGNRTCPRTQQVLSPHQSHSQSLDSRNDISVVC-NSG 60
           MRDPVILSTGET    FIQKWLNSGNRTCPRTQQVLS H   + +   R  IS  C   G
Sbjct: 86  MRDPVILSTGETYDRPFIQKWLNSGNRTCPRTQQVLS-HTDLTPNHLIREMISQWCATRG 145

Query: 61  NELQDRVRVHIL---------------------MTIS-----CKGAKNVDEANAFISSTF 120
            ELQDRVRVH L                     MTIS      K  + + +      + F
Sbjct: 146 IELQDRVRVHYLDDDVITEADRDRFLMLLEKMSMTISEQKEAAKELRMLTKRMPSFRALF 205

Query: 121 GESLDAISLCMS------------------------------------------------ 180
           GESLDAISL +S                                                
Sbjct: 206 GESLDAISLLLSPLSGDKSQSNSNIHTDLQEDVITTLLNLSIHDNNKKLVAETPYAIPLL 265

Query: 181 --------------MSCWLF--------------CNAIMP-------------------- 196
                          +  LF                A+ P                    
Sbjct: 266 MEALRSGTMETRSNAAAALFTLSALDPNKAIIGKAGALKPLIELLDLGHPLSIKDAASAI 325

BLAST of PI0009160 vs. NCBI nr
Match: KAA0068085.1 (U-box domain-containing protein 9 [Cucumis melo var. makuwa])

HSP 1 Score: 164.9 bits (416), Expect = 7.3e-37
Identity = 142/368 (38.59%), Postives = 154/368 (41.85%), Query Frame = 0

Query: 1   MRDPVILSTGET----FIQKWLNSGNRTCPRTQQVLSPHQSHSQSLDSRNDISVVC-NSG 60
           MRDPVILSTGET    FIQKWLNSGNRTCPRTQQVLS H   + +   R  IS  C   G
Sbjct: 86  MRDPVILSTGETYDRPFIQKWLNSGNRTCPRTQQVLS-HTDLTPNHLIREMISQWCATRG 145

Query: 61  NELQDRVRVHIL---------------------MTIS-----CKGAKNVDEANAFISSTF 120
            ELQDRVRVH L                     MTIS      K  + + +      + F
Sbjct: 146 IELQDRVRVHYLDDDVITEADRDRFLMLLEKMSMTISEQKEAAKELRMLTKRMPSFRALF 205

Query: 121 GESLDAISLCMS------------------------------------------------ 180
           GESLDAISL +S                                                
Sbjct: 206 GESLDAISLLLSPLSGDKSQSNSNIHTDLQEDLITTLLNLSIHDNNKKLVAETPYAIPLL 265

Query: 181 --------------MSCWLF--------------CNAIMP-------------------- 196
                          +  LF                A+ P                    
Sbjct: 266 MEALRSGTMETRSNAAAALFTLSALDPNKAIIGKAGALKPLIELLDLGHPLSIKDAASAI 325

BLAST of PI0009160 vs. NCBI nr
Match: XP_023554208.1 (U-box domain-containing protein 9-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 155.6 bits (392), Expect = 4.4e-34
Identity = 133/366 (36.34%), Postives = 153/366 (41.80%), Query Frame = 0

Query: 1   MRDPVILSTGET----FIQKWLNSGNRTCPRTQQVLSPHQSHSQSLDSRNDISVVCNS-G 60
           MRDPV++STGET    FI KWL SGNRTCPRTQQVLS H + + +   R  IS  C S G
Sbjct: 85  MRDPVVVSTGETYDRPFIHKWLRSGNRTCPRTQQVLS-HTNLTPNHLIREMISQWCTSRG 144

Query: 61  NELQDRVRVHIL---------------------MTI-----SCKGAKNVDEANAFISSTF 120
            ELQDRVRVH L                     MTI     + K  + + +      + F
Sbjct: 145 IELQDRVRVHCLEDDVITEADRDRFLTLIENMSMTIPEQKAAAKELRMLTKRMPSFRALF 204

Query: 121 GESLDAISLCMSMSCW-------------------------------------------- 180
           GESLDAISL +S  C                                             
Sbjct: 205 GESLDAISLLLSPLCGDKSQSSIHADLQEDVITTLLNLSIHDNNKKLVAETPNVIPLLKE 264

Query: 181 ----------------LFCNAIMPS----------------------------------- 196
                           LF  + + S                                   
Sbjct: 265 ALRSGTMETRSNAAAALFTLSALDSNKALIGKSGALKPLIDLLDEGHPLSMKDAASAIFN 324

BLAST of PI0009160 vs. TAIR 10
Match: AT3G07360.1 (plant U-box 9 )

HSP 1 Score: 97.8 bits (242), Expect = 1.0e-20
Identity = 46/81 (56.79%), Postives = 66/81 (81.48%), Query Frame = 0

Query: 115 RAIEEIGEL-ELSPLTQNIRESSCSRNKENCIVIIHSVCLYDRTKWKDMREEEKCYRTIS 174
           +A+EE+GEL  +S L +  RES C RNKEN IVI+H++C  DRTKWK+++EEE  + TI+
Sbjct: 370 KAVEELGELGGVSWLLKITRESECKRNKENAIVILHTICFSDRTKWKEIKEEENAHGTIT 429

Query: 175 ELAQNGTSRAKRKASGILERI 195
           +L++ GTSRA+RKA+GIL+R+
Sbjct: 430 KLSREGTSRAQRKANGILDRL 450


HSP 2 Score: 63.9 bits (154), Expect = 1.6e-10
Identity = 36/69 (52.17%), Postives = 48/69 (69.57%), Query Frame = 0

Query: 1   MRDPVILSTGET----FIQKWLNSGNRTCPRTQQVLSPHQSHSQSLDSRNDISVVCNSGN 60
           MRDPV+L++G+T    FIQKWL+SGNRTCP+TQQVL PH + + +L  R  IS  C   N
Sbjct: 87  MRDPVVLASGQTYDKLFIQKWLSSGNRTCPKTQQVL-PHTALTPNLLIREMISKWCKK-N 146

Query: 61  ELQDRVRVH 66
            L+ + + H
Sbjct: 147 GLETKSQYH 153

BLAST of PI0009160 vs. TAIR 10
Match: AT3G07360.2 (plant U-box 9 )

HSP 1 Score: 97.8 bits (242), Expect = 1.0e-20
Identity = 46/81 (56.79%), Postives = 66/81 (81.48%), Query Frame = 0

Query: 115 RAIEEIGEL-ELSPLTQNIRESSCSRNKENCIVIIHSVCLYDRTKWKDMREEEKCYRTIS 174
           +A+EE+GEL  +S L +  RES C RNKEN IVI+H++C  DRTKWK+++EEE  + TI+
Sbjct: 235 KAVEELGELGGVSWLLKITRESECKRNKENAIVILHTICFSDRTKWKEIKEEENAHGTIT 294

Query: 175 ELAQNGTSRAKRKASGILERI 195
           +L++ GTSRA+RKA+GIL+R+
Sbjct: 295 KLSREGTSRAQRKANGILDRL 315

BLAST of PI0009160 vs. TAIR 10
Match: AT3G07360.3 (plant U-box 9 )

HSP 1 Score: 97.8 bits (242), Expect = 1.0e-20
Identity = 46/81 (56.79%), Postives = 66/81 (81.48%), Query Frame = 0

Query: 115 RAIEEIGEL-ELSPLTQNIRESSCSRNKENCIVIIHSVCLYDRTKWKDMREEEKCYRTIS 174
           +A+EE+GEL  +S L +  RES C RNKEN IVI+H++C  DRTKWK+++EEE  + TI+
Sbjct: 235 KAVEELGELGGVSWLLKITRESECKRNKENAIVILHTICFSDRTKWKEIKEEENAHGTIT 294

Query: 175 ELAQNGTSRAKRKASGILERI 195
           +L++ GTSRA+RKA+GIL+R+
Sbjct: 295 KLSREGTSRAQRKANGILDRL 315

BLAST of PI0009160 vs. TAIR 10
Match: AT3G46510.1 (plant U-box 13 )

HSP 1 Score: 48.5 bits (114), Expect = 7.1e-06
Identity = 20/37 (54.05%), Postives = 29/37 (78.38%), Query Frame = 0

Query: 1   MRDPVILSTGETF----IQKWLNSGNRTCPRTQQVLS 34
           MRDPVI+S+G+T+    I+KW+  G+ TCP+TQQ L+
Sbjct: 269 MRDPVIVSSGQTYERTCIEKWIEGGHSTCPKTQQALT 305

BLAST of PI0009160 vs. TAIR 10
Match: AT1G23030.1 (ARM repeat superfamily protein )

HSP 1 Score: 48.1 bits (113), Expect = 9.2e-06
Identity = 51/199 (25.63%), Postives = 91/199 (45.73%), Query Frame = 0

Query: 1   MRDPVILSTGET----FIQKWLNSGNRTCPRTQQVLSPHQSHSQSLDSRNDISVVCNSGN 60
           M+DPVI++TG+T    +IQ+W++ GN TCP+TQQ L  + + + +   R+ IS  C   N
Sbjct: 254 MKDPVIVATGQTYERAYIQRWIDCGNLTCPKTQQKLE-NFTLTPNYVLRSLISRWCAEHN 313

Query: 61  ELQDRVRVHILMTISCKGAKNVDEANAFISSTFGESLD----AISLCMSMSCWLFCNAIM 120
             Q    ++       K + ++    A +      S +    A+S   S+S     N I+
Sbjct: 314 IEQPAGYIN----GRTKNSGDMSVIRALVQRLSSRSTEDRRNAVSEIRSLSKRSTDNRIL 373

Query: 121 PSRAIEEIGELELSPLTQNIRESSCSRNKENCIVIIHSVCLYDRTKWKDMREEEKCYRTI 180
                  I E    P+  N+  S     +EN I  + ++ +Y+    K++        +I
Sbjct: 374 -------IAEAGAIPVLVNLLTSEDVATQENAITCVLNLSIYENN--KELIMFAGAVTSI 433

Query: 181 SELAQNGTSRAKRKASGIL 192
            ++ + GT  A+  A+  L
Sbjct: 434 VQVLRAGTMEARENAAATL 438

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SRT01.4e-1956.79U-box domain-containing protein 9 OS=Arabidopsis thaliana OX=3702 GN=PUB9 PE=1 S... [more]
Q5VRH94.0e-0627.50U-box domain-containing protein 12 OS=Oryza sativa subsp. japonica OX=39947 GN=P... [more]
Q9SNC69.9e-0554.05U-box domain-containing protein 13 OS=Arabidopsis thaliana OX=3702 GN=PUB13 PE=1... [more]
Q8VZ401.3e-0423.23U-box domain-containing protein 14 OS=Arabidopsis thaliana OX=3702 GN=PUB14 PE=1... [more]
Q681N21.3e-0441.67U-box domain-containing protein 15 OS=Arabidopsis thaliana OX=3702 GN=PUB15 PE=2... [more]
Match NameE-valueIdentityDescription
A0A0A0L8U14.2e-3838.86RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_3G634320 PE... [more]
A0A5A7VQS83.5e-3738.59RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C... [more]
A0A5D3DRP93.5e-3738.59RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
A0A1S3CDM93.5e-3738.59RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103499224 PE=4 ... [more]
A0A6J1HKK52.1e-3436.34RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111463870... [more]
Match NameE-valueIdentityDescription
XP_004144394.18.6e-3838.86U-box domain-containing protein 9 [Cucumis sativus] >KGN58380.1 hypothetical pro... [more]
XP_038888241.17.3e-3738.15U-box domain-containing protein 9 [Benincasa hispida][more]
XP_008460394.17.3e-3738.59PREDICTED: U-box domain-containing protein 9 [Cucumis melo] >TYK26069.1 U-box do... [more]
KAA0068085.17.3e-3738.59U-box domain-containing protein 9 [Cucumis melo var. makuwa][more]
XP_023554208.14.4e-3436.34U-box domain-containing protein 9-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT3G07360.11.0e-2056.79plant U-box 9 [more]
AT3G07360.21.0e-2056.79plant U-box 9 [more]
AT3G07360.31.0e-2056.79plant U-box 9 [more]
AT3G46510.17.1e-0654.05plant U-box 13 [more]
AT1G23030.19.2e-0625.63ARM repeat superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003613U box domainSMARTSM00504Ubox_2coord: 1..51
e-value: 0.0026
score: 19.9
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 1..57
e-value: 9.5E-7
score: 30.8
NoneNo IPR availablePANTHERPTHR23315:SF253U-BOX DOMAIN-CONTAINING PROTEIN 9coord: 114..195
NoneNo IPR availablePANTHERPTHR23315U BOX DOMAIN-CONTAININGcoord: 1..63
NoneNo IPR availablePANTHERPTHR23315U BOX DOMAIN-CONTAININGcoord: 114..195
NoneNo IPR availablePANTHERPTHR23315:SF253U-BOX DOMAIN-CONTAINING PROTEIN 9coord: 1..63
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 1..36

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0009160.1PI0009160.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016567 protein ubiquitination
molecular_function GO:0004842 ubiquitin-protein transferase activity