PI0008337 (gene) Melon (PI 482460) v1

Overview
NamePI0008337
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
Description(1->3)-beta-glucan endohydrolase
Locationchr04: 10158975 .. 10161479 (-)
RNA-Seq ExpressionPI0008337
SyntenyPI0008337
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGAAGATTAGGGTTCTTGAAGTGGGTTTTGTTGGTTGGGGTTTTGGGTTCTTGTGTTGAAGGTCTTGGTGTTAATTGGGGAACCATGGCCAATCATAGATTACCCCCTAAGACTATTGTGCAGATGTTTAAGGATAATGGGATTCAGAAAGTGAAGCTGTTTGATGCAGATCAATCCTCCATGGGTGCTCTGGCTGGGACTGGTATTGAGGTCATGGTTGCCATTCCTAATGATCAACTTTCTGCCATGGGAGACTACAATCGAGCTAAGCAATGGGTGCAGAGGAATGTCACCCGTTACATCTTTGATGGAGGAGTTACCATCAAGTAATTCATTCCACTTTTTATAACTGTTTCAAACCATGGAAAATTGATTGTGTTTCCTTTCTTTTGATCTTTTCTGTTTTATTTTTATACTAAGCCTGTTCTGTTATTATTTGGATCTTGCAAATCCTCATCCCATGTAGATAGTTATGTTATTTCTATATCTGAGGAAGTCTCTTGGTTTGCATATACGTGGGCAACTTTGCAATCATTTGGAGTTGTTTGAATTTGTTTATTTGTGGTTGAAATAAGTCTCGTATATTTATTAGCAATCTGATAAACATGGTTTGAAATTTTTTCCTGAGATGGGTTTTGGAAATTCATTTTGAAATTCTTTTTCATTTTCATAAGCATTGACTTTTCTGATTAAGTTAATCATATAATAATACATAGATCGTTGGCTTTCCAGCTTCAGCAAAGGGTGAAAAGTTCTTTAACTGGTTACGAACAGGTTGTGGATGTGGTTTACTTTGACCATGAATCCTTGAATGGGTCGATGTATTCTGCTCATGAATTCAGCTCAGTCGTTTTCCTATGGAATTTTATCTTGTGTTTTTTCAAATAGATCAACAATATGTATAATTATAGTGCATGCCAAATCTTTTCTTTAAATATATGCCTGTCGTTTTCTTATTTATTAAATACAAAGATTAAAGTACAGTTAGCAATTTCTTTGTGCAAATGTTCTTGCAAGTGACTAATTTTATCTCCTTGGATGGAACTTGTTCTCTTTTACTATGGAGTCCTTAAGGTATAAAAGCCCTTTTGGTGGAGATTCTTGTATTTCTCGCTGCATCAACTTCATCCAATTTCTCACAAGGAACACATATGAAATATTTTATCAGCCTGCACATAAATAAGTAAATCATGAAAAACAAACAAAAAAAGAAACTGTGTTTGAGTACTTAGCTTTGATCAAACTCTTGGGAAGTGTCGTTATCTTTGATTCGAGTTGTTCACTTTCTGTTGAATTTTAAATTAGAAGATAGGATTGGTTTACGATTGTTGATTTTGTCTTGGGGTTATGAGCATAAAACTAACCTTTTCTTACTATGCTGATCTTGTTCAGATATGTTGCTGTTGGCAATGAACCATTTCTGTCATCCTATAATGGATCATTCTTGAACGTTACCTTTCCAGCTCTTCAAAACATTCAAAATGCACTCAATGAAGCAGGTCATGGAGATTCCATCAAGGCAACGGTGCCCTTAAATGCTGATGTTTACAACTCCCCAGTTAATAGCCCGTACCCGTCTGCTGGTAGATTTCGCAATGATATTAATCAACTCATGACTGATATTGTCCAGTTTCTAAACAAGAACAAGGCACCTTTTACTGTAAACATTTACCCTTTCTTAGTCTTTATGGCAACGACAACTTCCCTTTCGACTATGCTTTCTTTGATGGTGCAAGTAATCCTGTTGTTGACATTGGAACTGGGATTCAGTATACCAATGTATTTGATGCCAACTTCGACACGTTAGTTTCGTCGCTGAAGGCTGTTGGACTTGGGGACATGCCCATTCTAGTTGGTGAGGTTGGATGGCCAACTGATGGCGACAAGAATGCCAATGCTGGTAATGCTTACAGATTTTACAATGGCCTTCTACCAAGGCTTGCAGCCAACAAGGGCACTCCACTGAGGCCCGGTTTCATTGAAGTATACTTGTTCAGTTTTCTGGATGAAAATGGCAAGAGCATTGCTCCAGGAAATTTCGAACGGCATTGGGGTATTTTCGGGTATGACGGTCAGCCAAAGTTTGGCATGGATCTTTCTGGTCAGGGCCAAAACAAATTACTTGTGGGAGCACAGAATGTTCAGTATCTTCCTCAAAAGTGGTGTATGTTCAATCCAAATGCAAAGGATCTAAGTAAACTTGCTGATAACATAGATTATGCCTGCACTTTTTCTGATTGCACTTCCCTTGGATATGGATCTTCTTGCAATAATTTGGATGCTAATGGGAACGCGTCCTATGCATTCAATATGTATTTCCAGGTGCAAAATCAAGATGAATTGGCATGTAATTTCCAAGGTTTAGCCACAATAACCTCACAGAATCTTTCTCAAGGTACTTGCAATTTCATCATCCAGCTAGGTTCCTCGCCTCCGTCCCATGCTGCTTCATTTGTTAGTTTAGCATCTATGGTTTTGTTATTCATAGCACTATTGCTATAA

mRNA sequence

ATGGGAAGATTAGGGTTCTTGAAGTGGGTTTTGTTGGTTGGGGTTTTGGGTTCTTGTGTTGAAGGTCTTGGTGTTAATTGGGGAACCATGGCCAATCATAGATTACCCCCTAAGACTATTGTGCAGATGTTTAAGGATAATGGGATTCAGAAAGTGAAGCTGTTTGATGCAGATCAATCCTCCATGGGTGCTCTGGCTGGGACTGGTATTGAGGTCATGGTTGCCATTCCTAATGATCAACTTTCTGCCATGGGAGACTACAATCGAGCTAAGCAATGGGTGCAGAGGAATGTCACCCGTTACATCTTTGATGGAGGAGTTACCATCAAATATGTTGCTGTTGGCAATGAACCATTTCTGTCATCCTATAATGGATCATTCTTGAACGTTACCTTTCCAGCTCTTCAAAACATTCAAAATGCACTCAATGAAGCAGGTCATGGAGATTCCATCAAGGCAACGGTGCCCTTAAATGCTGATGTTTACAACTCCCCAGTTAATAGCCCGTACCCGTCTGCTGGTAGATTTCGCAATGATATTAATCAACTCATGACTGATATTGTCCATCTTTATGGCAACGACAACTTCCCTTTCGACTATGCTTTCTTTGATGGTGCAAGTAATCCTGTTGTTGACATTGGAACTGGGATTCAGTATACCAATGTATTTGATGCCAACTTCGACACGTTAGTTTCGTCGCTGAAGGCTGTTGGACTTGGGGACATGCCCATTCTAGTTGGTGAGGTTGGATGGCCAACTGATGGCGACAAGAATGCCAATGCTGGTAATGCTTACAGATTTTACAATGGCCTTCTACCAAGGCTTGCAGCCAACAAGGGCACTCCACTGAGGCCCGGTTTCATTGAAGTATACTTGTTCAGTTTTCTGGATGAAAATGGCAAGAGCATTGCTCCAGGAAATTTCGAACGGCATTGGGGTATTTTCGGGTATGACGGTCAGCCAAAGTTTGGCATGGATCTTTCTGGTCAGGGCCAAAACAAATTACTTGTGGGAGCACAGAATGTTCAGTATCTTCCTCAAAAGTGGTGTATGTTCAATCCAAATGCAAAGGATCTAAGTAAACTTGCTGATAACATAGATTATGCCTGCACTTTTTCTGATTGCACTTCCCTTGGATATGGATCTTCTTGCAATAATTTGGATGCTAATGGGAACGCGTCCTATGCATTCAATATGTATTTCCAGGTGCAAAATCAAGATGAATTGGCATGTAATTTCCAAGGTTTAGCCACAATAACCTCACAGAATCTTTCTCAAGGTACTTGCAATTTCATCATCCAGCTAGGTTCCTCGCCTCCGTCCCATGCTGCTTCATTTGTTAGTTTAGCATCTATGGTTTTGTTATTCATAGCACTATTGCTATAA

Coding sequence (CDS)

ATGGGAAGATTAGGGTTCTTGAAGTGGGTTTTGTTGGTTGGGGTTTTGGGTTCTTGTGTTGAAGGTCTTGGTGTTAATTGGGGAACCATGGCCAATCATAGATTACCCCCTAAGACTATTGTGCAGATGTTTAAGGATAATGGGATTCAGAAAGTGAAGCTGTTTGATGCAGATCAATCCTCCATGGGTGCTCTGGCTGGGACTGGTATTGAGGTCATGGTTGCCATTCCTAATGATCAACTTTCTGCCATGGGAGACTACAATCGAGCTAAGCAATGGGTGCAGAGGAATGTCACCCGTTACATCTTTGATGGAGGAGTTACCATCAAATATGTTGCTGTTGGCAATGAACCATTTCTGTCATCCTATAATGGATCATTCTTGAACGTTACCTTTCCAGCTCTTCAAAACATTCAAAATGCACTCAATGAAGCAGGTCATGGAGATTCCATCAAGGCAACGGTGCCCTTAAATGCTGATGTTTACAACTCCCCAGTTAATAGCCCGTACCCGTCTGCTGGTAGATTTCGCAATGATATTAATCAACTCATGACTGATATTGTCCATCTTTATGGCAACGACAACTTCCCTTTCGACTATGCTTTCTTTGATGGTGCAAGTAATCCTGTTGTTGACATTGGAACTGGGATTCAGTATACCAATGTATTTGATGCCAACTTCGACACGTTAGTTTCGTCGCTGAAGGCTGTTGGACTTGGGGACATGCCCATTCTAGTTGGTGAGGTTGGATGGCCAACTGATGGCGACAAGAATGCCAATGCTGGTAATGCTTACAGATTTTACAATGGCCTTCTACCAAGGCTTGCAGCCAACAAGGGCACTCCACTGAGGCCCGGTTTCATTGAAGTATACTTGTTCAGTTTTCTGGATGAAAATGGCAAGAGCATTGCTCCAGGAAATTTCGAACGGCATTGGGGTATTTTCGGGTATGACGGTCAGCCAAAGTTTGGCATGGATCTTTCTGGTCAGGGCCAAAACAAATTACTTGTGGGAGCACAGAATGTTCAGTATCTTCCTCAAAAGTGGTGTATGTTCAATCCAAATGCAAAGGATCTAAGTAAACTTGCTGATAACATAGATTATGCCTGCACTTTTTCTGATTGCACTTCCCTTGGATATGGATCTTCTTGCAATAATTTGGATGCTAATGGGAACGCGTCCTATGCATTCAATATGTATTTCCAGGTGCAAAATCAAGATGAATTGGCATGTAATTTCCAAGGTTTAGCCACAATAACCTCACAGAATCTTTCTCAAGGTACTTGCAATTTCATCATCCAGCTAGGTTCCTCGCCTCCGTCCCATGCTGCTTCATTTGTTAGTTTAGCATCTATGGTTTTGTTATTCATAGCACTATTGCTATAA

Protein sequence

MGRLGFLKWVLLVGVLGSCVEGLGVNWGTMANHRLPPKTIVQMFKDNGIQKVKLFDADQSSMGALAGTGIEVMVAIPNDQLSAMGDYNRAKQWVQRNVTRYIFDGGVTIKYVAVGNEPFLSSYNGSFLNVTFPALQNIQNALNEAGHGDSIKATVPLNADVYNSPVNSPYPSAGRFRNDINQLMTDIVHLYGNDNFPFDYAFFDGASNPVVDIGTGIQYTNVFDANFDTLVSSLKAVGLGDMPILVGEVGWPTDGDKNANAGNAYRFYNGLLPRLAANKGTPLRPGFIEVYLFSFLDENGKSIAPGNFERHWGIFGYDGQPKFGMDLSGQGQNKLLVGAQNVQYLPQKWCMFNPNAKDLSKLADNIDYACTFSDCTSLGYGSSCNNLDANGNASYAFNMYFQVQNQDELACNFQGLATITSQNLSQGTCNFIIQLGSSPPSHAASFVSLASMVLLFIALLL
Homology
BLAST of PI0008337 vs. ExPASy Swiss-Prot
Match: Q6NKW9 (Glucan endo-1,3-beta-glucosidase 8 OS=Arabidopsis thaliana OX=3702 GN=At1g64760 PE=2 SV=2)

HSP 1 Score: 680.6 bits (1755), Expect = 1.2e-194
Identity = 336/468 (71.79%), Postives = 383/468 (81.84%), Query Frame = 0

Query: 9   WVLLVGVLGSCVEGLGVNWGTMANHRLPPKTIVQMFKDNGIQKVKLFDADQSSMGALAGT 68
           +V+++G LG  V GLGVNWGTMA H+LPPKT+VQM KDN I KVKLFDAD+++MGALAG+
Sbjct: 11  FVIVIGHLGILVNGLGVNWGTMATHKLPPKTVVQMLKDNNINKVKLFDADETTMGALAGS 70

Query: 69  GIEVMVAIPNDQLSAMGDYNRAKQWVQRNVTRYIFDGGVTIKYVAVGNEPFLSSYNGSFL 128
           G+EVMVAIPNDQL  M  Y+RAK WV++NVTRY FDGGV I +VAVGNEPFL SYNGSF+
Sbjct: 71  GLEVMVAIPNDQLKVMTSYDRAKDWVRKNVTRYNFDGGVNITFVAVGNEPFLKSYNGSFI 130

Query: 129 NVTFPALQNIQNALNEAGHGDSIKATVPLNADVYNSPVNSPYPSAGRFRNDINQLMTDIV 188
           N+TFPAL NIQNALNEAG G+S+KATVPLNADVY+SP ++P PSAGRFR DI   MT IV
Sbjct: 131 NLTFPALANIQNALNEAGLGNSVKATVPLNADVYDSPASNPVPSAGRFRPDIIGQMTQIV 190

Query: 189 ------------------HLYGNDNFPFDYAFFDGASNPVVDIGTGIQYTNVFDANFDTL 248
                              LYGND+FP +YAFFDGA  P+ D   GI YTNVFDANFDTL
Sbjct: 191 DFLGKNNAPITINIYPFLSLYGNDDFPLNYAFFDGA-EPIND--NGIDYTNVFDANFDTL 250

Query: 249 VSSLKAVGLGDMPILVGEVGWPTDGDKNANAGNAYRFYNGLLPRLAANKGTPLRPGFIEV 308
           VSSLKAVG GDMPI+VGEVGWPT+GDK+ANAG+AYRFYNGLLPRL  NKGTPLRP +IEV
Sbjct: 251 VSSLKAVGHGDMPIIVGEVGWPTEGDKHANAGSAYRFYNGLLPRLGTNKGTPLRPTYIEV 310

Query: 309 YLFSFLDENGKSIAPGNFERHWGIFGYDGQPKFGMDLSGQGQNKLLVGAQNVQYLPQKWC 368
           YLF  LDE+ KSIAPG FERHWGIF +DGQPKF +DLSGQGQ+K L+GAQNV YLP KWC
Sbjct: 311 YLFGLLDEDAKSIAPGPFERHWGIFKFDGQPKFPIDLSGQGQSKFLIGAQNVPYLPNKWC 370

Query: 369 MFNPNAKDLSKLADNIDYACTFSDCTSLGYGSSCNNLDANGNASYAFNMYFQVQNQDELA 428
            FNP AKDL+KLA NIDYACTFSDCT+LGYGSSCN LDANGNASYAFNM+FQV+NQDE A
Sbjct: 371 TFNPEAKDLTKLAANIDYACTFSDCTALGYGSSCNTLDANGNASYAFNMFFQVKNQDESA 430

Query: 429 CNFQGLATITSQNLSQGTCNFIIQL-GSSPPSHAASFVSLASMVLLFI 458
           C FQGLATIT+QN+SQG CNF IQ+  SS  S + S  SL  +++ F+
Sbjct: 431 CYFQGLATITTQNISQGQCNFPIQIVASSASSFSCSSYSLVVLIVWFL 475

BLAST of PI0008337 vs. ExPASy Swiss-Prot
Match: Q9M088 (Glucan endo-1,3-beta-glucosidase 5 OS=Arabidopsis thaliana OX=3702 GN=At4g31140 PE=2 SV=1)

HSP 1 Score: 449.1 bits (1154), Expect = 5.9e-125
Identity = 234/465 (50.32%), Postives = 312/465 (67.10%), Query Frame = 0

Query: 20  VEGLGVNWGTMANHRLPPKTIVQMFKDNGIQKVKLFDADQSSMGALAGTGIEVMVAIPND 79
           VEG+GVNWG+ A H LPP T+V++ ++NGIQKVKLF+AD + + AL+ TGI+VMV IPND
Sbjct: 24  VEGIGVNWGSQARHPLPPATVVRLLRENGIQKVKLFEADSAILKALSRTGIQVMVGIPND 83

Query: 80  QLSAM-GDYNRAKQWVQRNVTRYIFDGGVTIKYVAVGNEPFLSSYNGSFLNVTFPALQNI 139
            L+ + G    A++WV +NV+ ++   GV I+YVAVGNEPFL ++NG+F  +T PALQNI
Sbjct: 84  LLAPLAGSVAAAERWVSQNVSAHVSSNGVDIRYVAVGNEPFLKAFNGTFEGITLPALQNI 143

Query: 140 QNALNEAGHGDSIKATVPLNADVYNSPVNSPYPSAGRFRNDINQLMTDIV---------- 199
           Q+A+ +AG    +K TVPLNADVY S  N   PS G FR +I  LM +IV          
Sbjct: 144 QSAIIKAGLATQVKVTVPLNADVYQSASN--LPSDGDFRPEIRDLMLNIVKFLSDNGAPF 203

Query: 200 --------HLYGNDNFPFDYAFFDGASNPVVDIGTGIQYTNVFDANFDTLVSSLKAVGLG 259
                    LY + NFP ++AFFDG   P+ D   G  Y NV DAN+DTLV SL+  G G
Sbjct: 204 TINIYPFISLYNDPNFPVEFAFFDGTGTPIND--NGRIYDNVLDANYDTLVWSLQKNGFG 263

Query: 260 DMPILVGEVGWPTDGDKNANAGNAYRFYNGLLPRLAANKGTPLRPGFIEVYLFSFLDENG 319
           ++ I+VGEVGWPTDGDKNAN   A R+  G + R  ANKGTP+RPG ++ YLF  +DE+ 
Sbjct: 264 NLTIIVGEVGWPTDGDKNANLMYARRYNQGFMNRQKANKGTPMRPGAMDAYLFGLIDEDA 323

Query: 320 KSIAPGNFERHWGIFGYDGQPKFGMDL-SGQGQNKLLVGAQNVQYLPQKWCMFNPNAK-D 379
           KSI PGNFERHWGIF  DGQPK+ + L SG G    L+ A++V YL +KWC+  PNA   
Sbjct: 324 KSIQPGNFERHWGIFYIDGQPKYQLSLGSGNG----LIPAKDVHYLAKKWCILAPNANLQ 383

Query: 380 LSKLADNIDYACTFSDCTSLGYGSSCNNLDANGNASYAFNMYFQVQNQDELACNFQGLAT 439
             +L  ++ YAC  +DCTSLGYGSSC NL+   N SYAFN Y+QV NQ + AC F GL+ 
Sbjct: 384 DPQLGPSVSYACDHADCTSLGYGSSCGNLNLAQNVSYAFNSYYQVSNQLDSACKFPGLSI 443

Query: 440 ITSQNLSQGTCNFIIQLGSSPPSHAASF--VSLASMVLLFIALLL 462
           +++++ S G+C F I + S   S A++   ++ ++ VLL +++ L
Sbjct: 444 VSTRDPSVGSCKFKIMIKSEDASEASAMMPITRSTAVLLLLSICL 480

BLAST of PI0008337 vs. ExPASy Swiss-Prot
Match: Q93Z08 (Glucan endo-1,3-beta-glucosidase 6 OS=Arabidopsis thaliana OX=3702 GN=At5g58090 PE=2 SV=2)

HSP 1 Score: 438.3 bits (1126), Expect = 1.0e-121
Identity = 220/451 (48.78%), Postives = 298/451 (66.08%), Query Frame = 0

Query: 5   GFLKWVLLVGVLGSCVEGLGVNWGTMANHRLPPKTIVQMFKDNGIQKVKLFDADQSSMGA 64
           G +  +L V +L      +G NWGT A+H LPP  +V+M ++NGIQKVKLFDA+  ++ A
Sbjct: 4   GSVLLLLAVALLCQRASSIGANWGTQASHPLPPDIVVRMLRENGIQKVKLFDAEYDTLRA 63

Query: 65  LAGTGIEVMVAIPNDQLSAM-GDYNRAKQWVQRNVTRYIFDGGVTIKYVAVGNEPFLSSY 124
           L  +GIEVMV IPN+ L+ +      A++WV +NV+ +I    V I+YVAVGNEPFLS+Y
Sbjct: 64  LGKSGIEVMVGIPNEMLATLASSLKAAEKWVAKNVSTHISTDNVNIRYVAVGNEPFLSTY 123

Query: 125 NGSFLNVTFPALQNIQNALNEAGHGDSIKATVPLNADVYNSPVNSPYPSAGRFRNDINQL 184
           NGS+L+ TFPAL+NIQ A+ +AG  + +K T PLNADVY+S  ++ +PS G FR +I  L
Sbjct: 124 NGSYLSTTFPALRNIQIAIIKAGLQNQVKVTCPLNADVYDS--STTFPSGGDFRANIRDL 183

Query: 185 MTDIV------------------HLYGNDNFPFDYAFFDGASNPVVDIGTGIQYTNVFDA 244
           M  IV                   LY N +FP DYAFFDG + P+ D GT   Y N+FDA
Sbjct: 184 MITIVKFLSENGGPFTVNIYPYISLYTNPDFPVDYAFFDGNAQPLNDGGT--FYYNMFDA 243

Query: 245 NFDTLVSSLKAVGLGDMPILVGEVGWPTDGDKNANAGNAYRFYNGLLPRLAANKGTPLRP 304
           N+DTLV +L+  G G+MPI++GE+GWPTDGD NAN   A +F  G +  ++  KGTP RP
Sbjct: 244 NYDTLVHALEKNGFGNMPIIIGEIGWPTDGDSNANLDYAKKFNQGFMAHISGGKGTPRRP 303

Query: 305 GFIEVYLFSFLDENGKSIAPGNFERHWGIFGYDGQPKFGMDLSGQGQNKLLVGAQNVQYL 364
           G I+ YLFS +DE+ KS+ PG FERHWGIF +DG PK+ ++L G      L+ A+ V+YL
Sbjct: 304 GPIDAYLFSLIDEDAKSVQPGYFERHWGIFTFDGLPKYALNL-GTTNTGALIQAKGVRYL 363

Query: 365 PQKWCMFNPNAK-DLSKLADNIDYACTFSDCTSLGYGSSCNNLDANGNASYAFNMYFQVQ 424
            +KWC+  PN + D  ++A  + YAC+  DCTSLG G+SC NLD   N SYAFN Y+Q+Q
Sbjct: 364 ERKWCVMKPNVRLDDPQVAPAVSYACSLGDCTSLGVGTSCANLDGKQNISYAFNSYYQIQ 423

Query: 425 NQDELACNFQGLATITSQNLSQGTCNFIIQL 436
           +Q + AC F  ++ +T  + S GTC F I +
Sbjct: 424 DQLDTACKFPNISEVTKTDPSTGTCRFPIMI 449

BLAST of PI0008337 vs. ExPASy Swiss-Prot
Match: Q9FGH4 (Glucan endo-1,3-beta-glucosidase 9 OS=Arabidopsis thaliana OX=3702 GN=At5g58480 PE=2 SV=1)

HSP 1 Score: 323.6 bits (828), Expect = 3.7e-87
Identity = 186/467 (39.83%), Postives = 263/467 (56.32%), Query Frame = 0

Query: 13  VGVLGSCVEGLGVNWGTMANHRLPPKTIVQMFKDNGIQKVKLFDADQSSMGALAGTGIEV 72
           + + G+ V  +G+NWGT A+H LPP  +V++ K NGI KVKLFDAD   + AL+G+ I V
Sbjct: 16  LSLTGTTVRAVGINWGTEASHPLPPSKVVELLKSNGIVKVKLFDADPKVLRALSGSNIGV 75

Query: 73  MVAIPNDQLSAM-GDYNRAKQWVQRNVTRYIFDGG--VTIKYVAVGNEPFLSSYNGSFLN 132
            + I N  L ++      A+ WV  NVTRY F+GG  V I+YVAVG EPFL SY   +  
Sbjct: 76  TIGIQNSMLKSLNASVKVAESWVHDNVTRY-FNGGNRVRIEYVAVGEEPFLQSYGNQYKP 135

Query: 133 VTFPALQNIQNALNEAGHGDSIKATVPLNADVYNSPVNSPYPSAGRFRNDINQLMTDI-- 192
               A  NIQNAL +A   + +K  VP + D + S   S  PS+G FR D+N+ M ++  
Sbjct: 136 FVIGAAMNIQNALVKANLANEVKVVVPSSFDSFLS--ESGRPSSGHFRADLNKTMIELLS 195

Query: 193 ----------------VHLYGNDNFPFDYAFFDGASNPVVDIGTGIQYTNVFDANFDTLV 252
                           +  + N N   D++ F   +    D      Y N FD ++DTLV
Sbjct: 196 FLTKHHSPFFVTISPFLSFHQNKNISLDFSLFKETAKAHKD--GRKTYRNSFDLSYDTLV 255

Query: 253 SSLKAVGLGDMPILVGEVGWPTDGDKNANAGNAYRFYNGLLPRLAANKGTPLRPGFIEVY 312
           S+L  +G  ++ I+V ++GWPTDG +NA +  A  F+ GL+  L     +  RP  +E Y
Sbjct: 256 SALFTIGFSEVDIVVSKIGWPTDGAENATSLTAEAFFKGLIVHLEKKTASLPRPP-VETY 315

Query: 313 LFSFLDENGKSIAPGNFERHWGIFGYDGQPKFGMDLSGQGQNKLLVGAQNVQYLPQKWCM 372
           + S LDE+ ++++ GNFERHWG+F +DGQ K+    + + Q    V AQNVQYLP KWC+
Sbjct: 316 IESLLDEDQRNLSAGNFERHWGVFTFDGQAKYNFSFNHKNQ----VNAQNVQYLPPKWCV 375

Query: 373 FNPNAKDLSKLADNIDYACTFSDCTSLGYGSSCNNLDANGNASYAFNMYFQVQNQDELAC 432
            N N KDLS  +     AC  +DCTS+  G SC+ +   GN SYAFN  +Q  +    +C
Sbjct: 376 VN-NNKDLSNASARALEACAVADCTSILPGGSCSGIRWPGNVSYAFNSLYQQNDHSAESC 435

Query: 433 NFQGLATITSQNLSQGTCNFIIQLGSS-PPSHAASFVSLASMVLLFI 458
           NF GL  IT+ + S+  C F IQL +S   S   +F     ++LLF+
Sbjct: 436 NFGGLGLITTVDPSEDNCRFSIQLDTSHSSSQTPNFFQSWPLLLLFL 471

BLAST of PI0008337 vs. ExPASy Swiss-Prot
Match: Q94G86 (Glucan endo-1,3-beta-D-glucosidase OS=Olea europaea OX=4146 GN=OLE9 PE=1 SV=1)

HSP 1 Score: 202.6 bits (514), Expect = 9.6e-51
Identity = 140/435 (32.18%), Postives = 214/435 (49.20%), Query Frame = 0

Query: 23  LGVNWGTMANHRLPPKTIVQMFKDNGIQKVKLFDADQSSMGALAGTGIEVMVAIPNDQLS 82
           LGVN+G ++++    +  V + K   IQKV+LF A+ + + A A TG+E+++   N  + 
Sbjct: 30  LGVNYGQLSDNLPSLQATVNLLKSTTIQKVRLFGAEPAVIKAFANTGVEIVIGFDNGDIP 89

Query: 83  AM-GDYNRAKQWVQRNVTRYIFDGGVTIKYVAVGNEPFLSSYNGSFLNVTFPALQNIQNA 142
            +  + N A Q+V+ NV    F     I  + VGNE  L+S +   ++   PA+QN+QNA
Sbjct: 90  TLASNPNVASQFVKSNVMS--FYPASNIIAITVGNE-VLTSGDQKLISQLLPAMQNVQNA 149

Query: 143 LNEAGHGDSIKATVPLNADVYNSPVNSPYPSAGRFRNDINQLMTDIVHLYGNDNFPF--- 202
           LN A  G  +K +      V +    S  PS+G F   +   M  ++     ++ PF   
Sbjct: 150 LNAASLGGKVKVSTVHAMAVLS---QSYPPSSGVFNPGLGDTMKALLQFQSANDAPFMIS 209

Query: 203 DYAFFDGASNPV---------------VDIGTGIQYTNVFDANFDTLVSSLKAVGLGDMP 262
            Y +F   + P                VD G G +YTN+FDA  D + S+L A+G  D+ 
Sbjct: 210 PYPYFAYKNQPTPDTLAFCLFQPNAGQVDSGNGHKYTNMFDAQVDAVHSALNAMGFKDIE 269

Query: 263 ILVGEVGWPTDGDKN---ANAGNAYRFYNGLLPRLAANKGTPLRPG-FIEVYLFSFLDEN 322
           I+V E GWP  GD N    +  NA  +   L+  L +  GTPL PG  I+ YLFS  DE+
Sbjct: 270 IVVAETGWPHGGDSNEVGPSLDNAKAYVGNLINHLKSKVGTPLMPGKSIDTYLFSLYDED 329

Query: 323 GKSIAPGNFERHWGIFGYDGQPKFGMDLSGQGQNKLLVGAQN-VQYLPQKWCMFNPNAKD 382
            K+ A  + E+++G+F  DG   + + L    QN                WC+  P   D
Sbjct: 330 KKTGA--SSEKYFGLFKPDGSTTYDVGLLKNTQNPTTPATPTPTPKAAGSWCVPKPGVSD 389

Query: 383 LSKLADNIDYACTFS-DCTSLGYGSSCNNLD-ANGNASYAFNMYFQVQNQDELACNFQGL 432
             +L  NI+YAC    DC  +  G +C   +    +A+Y  N+Y+Q   ++   C+F   
Sbjct: 390 -DQLTGNINYACGQGIDCGPIQPGGACFEPNTVKAHAAYVMNLYYQSAGRNSWNCDFSQT 449

BLAST of PI0008337 vs. ExPASy TrEMBL
Match: A0A0A0LFN2 ((1->3)-beta-glucan endohydrolase OS=Cucumis sativus OX=3659 GN=Csa_3G843830 PE=3 SV=1)

HSP 1 Score: 917.9 bits (2371), Expect = 1.7e-263
Identity = 451/479 (94.15%), Postives = 454/479 (94.78%), Query Frame = 0

Query: 1   MGRLGFLKWVLLVGVLGSCVEGLGVNWGTMANHRLPPKTIVQMFKDNGIQKVKLFDADQS 60
           MG LGFLKWVLLVGVLGSCVEGLGVNWGTMANHRLPPKT+VQMFKDNGIQKVKLFDADQS
Sbjct: 1   MGSLGFLKWVLLVGVLGSCVEGLGVNWGTMANHRLPPKTVVQMFKDNGIQKVKLFDADQS 60

Query: 61  SMGALAGTGIEVMVAIPNDQLSAMGDYNRAKQWVQRNVTRYIFDGGVTIKYVAVGNEPFL 120
           SMGALAGTGIEVMVAIPNDQLSAMGDYNRAKQWVQRNVTRY FDGGVTIKYVAVGNEPFL
Sbjct: 61  SMGALAGTGIEVMVAIPNDQLSAMGDYNRAKQWVQRNVTRYTFDGGVTIKYVAVGNEPFL 120

Query: 121 SSYNGSFLNVTFPALQNIQNALNEAGHGDSIKATVPLNADVYNSPVNSPYPSAGRFRNDI 180
           SSYNGSFLNVTFPAL NIQNALNEAGHGDSIKATVPLNADVYNSPVNSPYPSAGRFRNDI
Sbjct: 121 SSYNGSFLNVTFPALLNIQNALNEAGHGDSIKATVPLNADVYNSPVNSPYPSAGRFRNDI 180

Query: 181 NQLMTDIVH------------------LYGNDNFPFDYAFFDGASNPVVDIGTGIQYTNV 240
           NQLMTDIV                   LYGNDNFPFDYAFFDGASNPVVDIGTGIQYTNV
Sbjct: 181 NQLMTDIVQFLNKNKAPFTVNIYPFLSLYGNDNFPFDYAFFDGASNPVVDIGTGIQYTNV 240

Query: 241 FDANFDTLVSSLKAVGLGDMPILVGEVGWPTDGDKNANAGNAYRFYNGLLPRLAANKGTP 300
           FDANFDTLVSSLKAVGLGDMPILVGEVGWPTDGDKN N GNAYRFYNGLLPRLAANKGTP
Sbjct: 241 FDANFDTLVSSLKAVGLGDMPILVGEVGWPTDGDKNGNVGNAYRFYNGLLPRLAANKGTP 300

Query: 301 LRPGFIEVYLFSFLDENGKSIAPGNFERHWGIFGYDGQPKFGMDLSGQGQNKLLVGAQNV 360
           LRPGFIEVYLFSFLDENGKSIAPGNFERHWGIFGYDGQPKFGMDLSGQGQNKLLVGAQNV
Sbjct: 301 LRPGFIEVYLFSFLDENGKSIAPGNFERHWGIFGYDGQPKFGMDLSGQGQNKLLVGAQNV 360

Query: 361 QYLPQKWCMFNPNAKDLSKLADNIDYACTFSDCTSLGYGSSCNNLDANGNASYAFNMYFQ 420
           QYLPQKWCMFNPNAKDLSKLADNIDYACTFSDCTSLGYGSSCNNLDANGNASYAFNMYFQ
Sbjct: 361 QYLPQKWCMFNPNAKDLSKLADNIDYACTFSDCTSLGYGSSCNNLDANGNASYAFNMYFQ 420

Query: 421 VQNQDELACNFQGLATITSQNLSQGTCNFIIQLGSSPPSHAASFVSLASMVLLFIALLL 462
           VQNQDELACNFQGLAT+TSQNLSQGTCNFIIQL SSPPSHAASFVSLASM+LLFIALLL
Sbjct: 421 VQNQDELACNFQGLATVTSQNLSQGTCNFIIQLASSPPSHAASFVSLASMILLFIALLL 479

BLAST of PI0008337 vs. ExPASy TrEMBL
Match: A0A5D3E281 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold734G00130 PE=3 SV=1)

HSP 1 Score: 917.5 bits (2370), Expect = 2.2e-263
Identity = 452/479 (94.36%), Postives = 456/479 (95.20%), Query Frame = 0

Query: 1   MGRLGFLKWVLLVGVLGSCVEGLGVNWGTMANHRLPPKTIVQMFKDNGIQKVKLFDADQS 60
           MGRLGFLKWVLLVGVLGSCVEGLGVNWGTMANHRLPPKT+VQM KDNGIQKVKLFDADQS
Sbjct: 1   MGRLGFLKWVLLVGVLGSCVEGLGVNWGTMANHRLPPKTVVQMLKDNGIQKVKLFDADQS 60

Query: 61  SMGALAGTGIEVMVAIPNDQLSAMGDYNRAKQWVQRNVTRYIFDGGVTIKYVAVGNEPFL 120
           SMGALAGTGIEVMVAIPNDQLSAMGDYNRAKQWVQRNVTRYIFDGGVTIKYVAVGNEPFL
Sbjct: 61  SMGALAGTGIEVMVAIPNDQLSAMGDYNRAKQWVQRNVTRYIFDGGVTIKYVAVGNEPFL 120

Query: 121 SSYNGSFLNVTFPALQNIQNALNEAGHGDSIKATVPLNADVYNSPVNSPYPSAGRFRNDI 180
           SSYNGSFLNVTFPALQNIQNALNEAGHGDSIKATVPLNADVYNSPVNSPYPSAGRFR+DI
Sbjct: 121 SSYNGSFLNVTFPALQNIQNALNEAGHGDSIKATVPLNADVYNSPVNSPYPSAGRFRSDI 180

Query: 181 NQLMTDIVH------------------LYGNDNFPFDYAFFDGASNPVVDIGTGIQYTNV 240
           NQLM DIV                   LYGNDNFPFDYAFFDGASNPVVDIGTGIQYTNV
Sbjct: 181 NQLMNDIVQFLNKNKAPFTVNIYPFLSLYGNDNFPFDYAFFDGASNPVVDIGTGIQYTNV 240

Query: 241 FDANFDTLVSSLKAVGLGDMPILVGEVGWPTDGDKNANAGNAYRFYNGLLPRLAANKGTP 300
           FDANFDTLVSSLKAVGLGDMPILVGEVGWPTDGDKNAN GNAYRFYNGLLPRLAANKGTP
Sbjct: 241 FDANFDTLVSSLKAVGLGDMPILVGEVGWPTDGDKNANDGNAYRFYNGLLPRLAANKGTP 300

Query: 301 LRPGFIEVYLFSFLDENGKSIAPGNFERHWGIFGYDGQPKFGMDLSGQGQNKLLVGAQNV 360
           LRPGFIEVYLFSFLDENGKSIAPGNFERHWGIFGYDGQPKFGMDLSGQGQNKLLVGAQNV
Sbjct: 301 LRPGFIEVYLFSFLDENGKSIAPGNFERHWGIFGYDGQPKFGMDLSGQGQNKLLVGAQNV 360

Query: 361 QYLPQKWCMFNPNAKDLSKLADNIDYACTFSDCTSLGYGSSCNNLDANGNASYAFNMYFQ 420
           +YLPQKWCMFNPNAKDLSKLADNIDYACTFSDCTSLGYGSSCNNLDANGNASYAFNMYFQ
Sbjct: 361 EYLPQKWCMFNPNAKDLSKLADNIDYACTFSDCTSLGYGSSCNNLDANGNASYAFNMYFQ 420

Query: 421 VQNQDELACNFQGLATITSQNLSQGTCNFIIQLGSSPPSHAASFVSLASMVLLFIALLL 462
           VQNQDELACNFQGLATITS+NLSQGTCNFIIQL SSPPSHAASFVSLASMVLLFIALLL
Sbjct: 421 VQNQDELACNFQGLATITSRNLSQGTCNFIIQLASSPPSHAASFVSLASMVLLFIALLL 479

BLAST of PI0008337 vs. ExPASy TrEMBL
Match: A0A1S3CNJ0 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo OX=3656 GN=LOC103502975 PE=3 SV=1)

HSP 1 Score: 917.5 bits (2370), Expect = 2.2e-263
Identity = 452/479 (94.36%), Postives = 456/479 (95.20%), Query Frame = 0

Query: 1   MGRLGFLKWVLLVGVLGSCVEGLGVNWGTMANHRLPPKTIVQMFKDNGIQKVKLFDADQS 60
           MGRLGFLKWVLLVGVLGSCVEGLGVNWGTMANHRLPPKT+VQM KDNGIQKVKLFDADQS
Sbjct: 1   MGRLGFLKWVLLVGVLGSCVEGLGVNWGTMANHRLPPKTVVQMLKDNGIQKVKLFDADQS 60

Query: 61  SMGALAGTGIEVMVAIPNDQLSAMGDYNRAKQWVQRNVTRYIFDGGVTIKYVAVGNEPFL 120
           SMGALAGTGIEVMVAIPNDQLSAMGDYNRAKQWVQRNVTRYIFDGGVTIKYVAVGNEPFL
Sbjct: 61  SMGALAGTGIEVMVAIPNDQLSAMGDYNRAKQWVQRNVTRYIFDGGVTIKYVAVGNEPFL 120

Query: 121 SSYNGSFLNVTFPALQNIQNALNEAGHGDSIKATVPLNADVYNSPVNSPYPSAGRFRNDI 180
           SSYNGSFLNVTFPALQNIQNALNEAGHGDSIKATVPLNADVYNSPVNSPYPSAGRFR+DI
Sbjct: 121 SSYNGSFLNVTFPALQNIQNALNEAGHGDSIKATVPLNADVYNSPVNSPYPSAGRFRSDI 180

Query: 181 NQLMTDIVH------------------LYGNDNFPFDYAFFDGASNPVVDIGTGIQYTNV 240
           NQLM DIV                   LYGNDNFPFDYAFFDGASNPVVDIGTGIQYTNV
Sbjct: 181 NQLMNDIVQFLNKNKAPFTVNIYPFLSLYGNDNFPFDYAFFDGASNPVVDIGTGIQYTNV 240

Query: 241 FDANFDTLVSSLKAVGLGDMPILVGEVGWPTDGDKNANAGNAYRFYNGLLPRLAANKGTP 300
           FDANFDTLVSSLKAVGLGDMPILVGEVGWPTDGDKNAN GNAYRFYNGLLPRLAANKGTP
Sbjct: 241 FDANFDTLVSSLKAVGLGDMPILVGEVGWPTDGDKNANDGNAYRFYNGLLPRLAANKGTP 300

Query: 301 LRPGFIEVYLFSFLDENGKSIAPGNFERHWGIFGYDGQPKFGMDLSGQGQNKLLVGAQNV 360
           LRPGFIEVYLFSFLDENGKSIAPGNFERHWGIFGYDGQPKFGMDLSGQGQNKLLVGAQNV
Sbjct: 301 LRPGFIEVYLFSFLDENGKSIAPGNFERHWGIFGYDGQPKFGMDLSGQGQNKLLVGAQNV 360

Query: 361 QYLPQKWCMFNPNAKDLSKLADNIDYACTFSDCTSLGYGSSCNNLDANGNASYAFNMYFQ 420
           +YLPQKWCMFNPNAKDLSKLADNIDYACTFSDCTSLGYGSSCNNLDANGNASYAFNMYFQ
Sbjct: 361 EYLPQKWCMFNPNAKDLSKLADNIDYACTFSDCTSLGYGSSCNNLDANGNASYAFNMYFQ 420

Query: 421 VQNQDELACNFQGLATITSQNLSQGTCNFIIQLGSSPPSHAASFVSLASMVLLFIALLL 462
           VQNQDELACNFQGLATITS+NLSQGTCNFIIQL SSPPSHAASFVSLASMVLLFIALLL
Sbjct: 421 VQNQDELACNFQGLATITSRNLSQGTCNFIIQLASSPPSHAASFVSLASMVLLFIALLL 479

BLAST of PI0008337 vs. ExPASy TrEMBL
Match: A0A6J1I9Q3 ((1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111472848 PE=3 SV=1)

HSP 1 Score: 877.5 bits (2266), Expect = 2.5e-251
Identity = 431/479 (89.98%), Postives = 443/479 (92.48%), Query Frame = 0

Query: 1   MGRLGFLKWVLLVGVLGSCVEGLGVNWGTMANHRLPPKTIVQMFKDNGIQKVKLFDADQS 60
           MGRL  L WVLLVGVLG C+EGLGVNWGTMANH+LPPKTIVQMFKDNGIQKVKLFDADQS
Sbjct: 1   MGRLWILNWVLLVGVLGCCIEGLGVNWGTMANHKLPPKTIVQMFKDNGIQKVKLFDADQS 60

Query: 61  SMGALAGTGIEVMVAIPNDQLSAMGDYNRAKQWVQRNVTRYIFDGGVTIKYVAVGNEPFL 120
           SMGALAGTGIEVMVAIPNDQL AMGDYNRAKQWVQRNV+RY FDGGVTIKYVAVGNEPFL
Sbjct: 61  SMGALAGTGIEVMVAIPNDQLVAMGDYNRAKQWVQRNVSRYTFDGGVTIKYVAVGNEPFL 120

Query: 121 SSYNGSFLNVTFPALQNIQNALNEAGHGDSIKATVPLNADVYNSPVNSPYPSAGRFRNDI 180
           SSYNGSFLNVTFPALQN+QNALNEAG+GD+IKATVPLNADVYNSPVN+PYPSAGRFR+DI
Sbjct: 121 SSYNGSFLNVTFPALQNVQNALNEAGYGDTIKATVPLNADVYNSPVNTPYPSAGRFRSDI 180

Query: 181 NQLMTDIVH------------------LYGNDNFPFDYAFFDGASNPVVDIGTGIQYTNV 240
           N LMT IV                   LYGNDNFPFDYAFFDGASNPVVD+ TGIQYTNV
Sbjct: 181 NGLMTQIVQFLSKNNAPFTVNIYPFLSLYGNDNFPFDYAFFDGASNPVVDVNTGIQYTNV 240

Query: 241 FDANFDTLVSSLKAVGLGDMPILVGEVGWPTDGDKNANAGNAYRFYNGLLPRLAANKGTP 300
           FDANFDTLVSSLKAVGLGDMPILVGEVGWPTDGDKNANAGNAYRFYNGLLPRLAANKGTP
Sbjct: 241 FDANFDTLVSSLKAVGLGDMPILVGEVGWPTDGDKNANAGNAYRFYNGLLPRLAANKGTP 300

Query: 301 LRPGFIEVYLFSFLDENGKSIAPGNFERHWGIFGYDGQPKFGMDLSGQGQNKLLVGAQNV 360
           LRPGFIEVYLFSFLDENGKSIAPGNFERHWGIFGYDGQPKFGMDLSGQGQNKLLVGAQ+V
Sbjct: 301 LRPGFIEVYLFSFLDENGKSIAPGNFERHWGIFGYDGQPKFGMDLSGQGQNKLLVGAQDV 360

Query: 361 QYLPQKWCMFNPNAKDLSKLADNIDYACTFSDCTSLGYGSSCNNLDANGNASYAFNMYFQ 420
           QYLPQKWCMFNPNAKDLSKLADNIDYACTFSDCTSLGYGSSCNNLDANGNASYAFNMYFQ
Sbjct: 361 QYLPQKWCMFNPNAKDLSKLADNIDYACTFSDCTSLGYGSSCNNLDANGNASYAFNMYFQ 420

Query: 421 VQNQDELACNFQGLATITSQNLSQGTCNFIIQLGSSPPSHAASFVSLASMVLLFIALLL 462
           VQNQDELACNFQGLATI+SQNLSQGTC FIIQL SS PS  ASFVSL SM LLF A+LL
Sbjct: 421 VQNQDELACNFQGLATISSQNLSQGTCKFIIQLASSSPSFDASFVSLVSMALLFTAMLL 479

BLAST of PI0008337 vs. ExPASy TrEMBL
Match: A0A6J1EXE0 ((1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111439258 PE=3 SV=1)

HSP 1 Score: 874.0 bits (2257), Expect = 2.7e-250
Identity = 429/477 (89.94%), Postives = 441/477 (92.45%), Query Frame = 0

Query: 1   MGRLGFLKWVLLVGVLGSCVEGLGVNWGTMANHRLPPKTIVQMFKDNGIQKVKLFDADQS 60
           MGRL  LKWVLLVGVLG C+EGLGVNWGTMANH+LPPKT+VQMFKDNGIQKVKLFDADQS
Sbjct: 1   MGRLWILKWVLLVGVLGCCIEGLGVNWGTMANHKLPPKTVVQMFKDNGIQKVKLFDADQS 60

Query: 61  SMGALAGTGIEVMVAIPNDQLSAMGDYNRAKQWVQRNVTRYIFDGGVTIKYVAVGNEPFL 120
           SMGALAGTGIEVMVAIPNDQL AMGDYNRAKQWVQRNV+RY FDGGVTIKYVAVGNEPFL
Sbjct: 61  SMGALAGTGIEVMVAIPNDQLVAMGDYNRAKQWVQRNVSRYTFDGGVTIKYVAVGNEPFL 120

Query: 121 SSYNGSFLNVTFPALQNIQNALNEAGHGDSIKATVPLNADVYNSPVNSPYPSAGRFRNDI 180
           SSYNGSFLNVTFPALQNIQNALNEAG+GD+IKATVPLNADVYNSPVN+PYPSAGRFR+DI
Sbjct: 121 SSYNGSFLNVTFPALQNIQNALNEAGYGDTIKATVPLNADVYNSPVNTPYPSAGRFRSDI 180

Query: 181 NQLMTDIVH------------------LYGNDNFPFDYAFFDGASNPVVDIGTGIQYTNV 240
           N LMT IV                   LYGNDNFPFDYAFFDGASNPVVD+ TGIQYTNV
Sbjct: 181 NGLMTQIVQFLSKNNAPFTVNIYPFLSLYGNDNFPFDYAFFDGASNPVVDMNTGIQYTNV 240

Query: 241 FDANFDTLVSSLKAVGLGDMPILVGEVGWPTDGDKNANAGNAYRFYNGLLPRLAANKGTP 300
           FDANFDTLVSSLKAVGLGDMPILVGEVGWPTDGDKNANAGNAYRFYNGLLPRLAANKGTP
Sbjct: 241 FDANFDTLVSSLKAVGLGDMPILVGEVGWPTDGDKNANAGNAYRFYNGLLPRLAANKGTP 300

Query: 301 LRPGFIEVYLFSFLDENGKSIAPGNFERHWGIFGYDGQPKFGMDLSGQGQNKLLVGAQNV 360
           LRPGFIEVYLFSFLDENGKSIAPGNFERHWGIFGYDGQPKFGMDLSGQGQNKLLVGAQ+V
Sbjct: 301 LRPGFIEVYLFSFLDENGKSIAPGNFERHWGIFGYDGQPKFGMDLSGQGQNKLLVGAQDV 360

Query: 361 QYLPQKWCMFNPNAKDLSKLADNIDYACTFSDCTSLGYGSSCNNLDANGNASYAFNMYFQ 420
           QYLPQKWCMFNPNAKDLSKLADNIDYACTFSDCTSLGYGSSCNNLDANGNASYAFNMYFQ
Sbjct: 361 QYLPQKWCMFNPNAKDLSKLADNIDYACTFSDCTSLGYGSSCNNLDANGNASYAFNMYFQ 420

Query: 421 VQNQDELACNFQGLATITSQNLSQGTCNFIIQLGSSPPSHAASFVSLASMVLLFIAL 460
           VQNQDELACNFQGLAT+TSQNLSQGTC FIIQL SS PS   SFVSL SM LLF A+
Sbjct: 421 VQNQDELACNFQGLATMTSQNLSQGTCKFIIQLASSSPSLDDSFVSLVSMALLFTAM 477

BLAST of PI0008337 vs. NCBI nr
Match: XP_004141142.1 (glucan endo-1,3-beta-glucosidase 8 [Cucumis sativus] >KGN59764.1 hypothetical protein Csa_001412 [Cucumis sativus])

HSP 1 Score: 917.9 bits (2371), Expect = 3.4e-263
Identity = 451/479 (94.15%), Postives = 454/479 (94.78%), Query Frame = 0

Query: 1   MGRLGFLKWVLLVGVLGSCVEGLGVNWGTMANHRLPPKTIVQMFKDNGIQKVKLFDADQS 60
           MG LGFLKWVLLVGVLGSCVEGLGVNWGTMANHRLPPKT+VQMFKDNGIQKVKLFDADQS
Sbjct: 1   MGSLGFLKWVLLVGVLGSCVEGLGVNWGTMANHRLPPKTVVQMFKDNGIQKVKLFDADQS 60

Query: 61  SMGALAGTGIEVMVAIPNDQLSAMGDYNRAKQWVQRNVTRYIFDGGVTIKYVAVGNEPFL 120
           SMGALAGTGIEVMVAIPNDQLSAMGDYNRAKQWVQRNVTRY FDGGVTIKYVAVGNEPFL
Sbjct: 61  SMGALAGTGIEVMVAIPNDQLSAMGDYNRAKQWVQRNVTRYTFDGGVTIKYVAVGNEPFL 120

Query: 121 SSYNGSFLNVTFPALQNIQNALNEAGHGDSIKATVPLNADVYNSPVNSPYPSAGRFRNDI 180
           SSYNGSFLNVTFPAL NIQNALNEAGHGDSIKATVPLNADVYNSPVNSPYPSAGRFRNDI
Sbjct: 121 SSYNGSFLNVTFPALLNIQNALNEAGHGDSIKATVPLNADVYNSPVNSPYPSAGRFRNDI 180

Query: 181 NQLMTDIVH------------------LYGNDNFPFDYAFFDGASNPVVDIGTGIQYTNV 240
           NQLMTDIV                   LYGNDNFPFDYAFFDGASNPVVDIGTGIQYTNV
Sbjct: 181 NQLMTDIVQFLNKNKAPFTVNIYPFLSLYGNDNFPFDYAFFDGASNPVVDIGTGIQYTNV 240

Query: 241 FDANFDTLVSSLKAVGLGDMPILVGEVGWPTDGDKNANAGNAYRFYNGLLPRLAANKGTP 300
           FDANFDTLVSSLKAVGLGDMPILVGEVGWPTDGDKN N GNAYRFYNGLLPRLAANKGTP
Sbjct: 241 FDANFDTLVSSLKAVGLGDMPILVGEVGWPTDGDKNGNVGNAYRFYNGLLPRLAANKGTP 300

Query: 301 LRPGFIEVYLFSFLDENGKSIAPGNFERHWGIFGYDGQPKFGMDLSGQGQNKLLVGAQNV 360
           LRPGFIEVYLFSFLDENGKSIAPGNFERHWGIFGYDGQPKFGMDLSGQGQNKLLVGAQNV
Sbjct: 301 LRPGFIEVYLFSFLDENGKSIAPGNFERHWGIFGYDGQPKFGMDLSGQGQNKLLVGAQNV 360

Query: 361 QYLPQKWCMFNPNAKDLSKLADNIDYACTFSDCTSLGYGSSCNNLDANGNASYAFNMYFQ 420
           QYLPQKWCMFNPNAKDLSKLADNIDYACTFSDCTSLGYGSSCNNLDANGNASYAFNMYFQ
Sbjct: 361 QYLPQKWCMFNPNAKDLSKLADNIDYACTFSDCTSLGYGSSCNNLDANGNASYAFNMYFQ 420

Query: 421 VQNQDELACNFQGLATITSQNLSQGTCNFIIQLGSSPPSHAASFVSLASMVLLFIALLL 462
           VQNQDELACNFQGLAT+TSQNLSQGTCNFIIQL SSPPSHAASFVSLASM+LLFIALLL
Sbjct: 421 VQNQDELACNFQGLATVTSQNLSQGTCNFIIQLASSPPSHAASFVSLASMILLFIALLL 479

BLAST of PI0008337 vs. NCBI nr
Match: XP_008465328.1 (PREDICTED: glucan endo-1,3-beta-glucosidase 8-like [Cucumis melo] >KAA0051277.1 glucan endo-1,3-beta-glucosidase 8-like [Cucumis melo var. makuwa] >TYK29984.1 glucan endo-1,3-beta-glucosidase 8-like [Cucumis melo var. makuwa])

HSP 1 Score: 917.5 bits (2370), Expect = 4.5e-263
Identity = 452/479 (94.36%), Postives = 456/479 (95.20%), Query Frame = 0

Query: 1   MGRLGFLKWVLLVGVLGSCVEGLGVNWGTMANHRLPPKTIVQMFKDNGIQKVKLFDADQS 60
           MGRLGFLKWVLLVGVLGSCVEGLGVNWGTMANHRLPPKT+VQM KDNGIQKVKLFDADQS
Sbjct: 1   MGRLGFLKWVLLVGVLGSCVEGLGVNWGTMANHRLPPKTVVQMLKDNGIQKVKLFDADQS 60

Query: 61  SMGALAGTGIEVMVAIPNDQLSAMGDYNRAKQWVQRNVTRYIFDGGVTIKYVAVGNEPFL 120
           SMGALAGTGIEVMVAIPNDQLSAMGDYNRAKQWVQRNVTRYIFDGGVTIKYVAVGNEPFL
Sbjct: 61  SMGALAGTGIEVMVAIPNDQLSAMGDYNRAKQWVQRNVTRYIFDGGVTIKYVAVGNEPFL 120

Query: 121 SSYNGSFLNVTFPALQNIQNALNEAGHGDSIKATVPLNADVYNSPVNSPYPSAGRFRNDI 180
           SSYNGSFLNVTFPALQNIQNALNEAGHGDSIKATVPLNADVYNSPVNSPYPSAGRFR+DI
Sbjct: 121 SSYNGSFLNVTFPALQNIQNALNEAGHGDSIKATVPLNADVYNSPVNSPYPSAGRFRSDI 180

Query: 181 NQLMTDIVH------------------LYGNDNFPFDYAFFDGASNPVVDIGTGIQYTNV 240
           NQLM DIV                   LYGNDNFPFDYAFFDGASNPVVDIGTGIQYTNV
Sbjct: 181 NQLMNDIVQFLNKNKAPFTVNIYPFLSLYGNDNFPFDYAFFDGASNPVVDIGTGIQYTNV 240

Query: 241 FDANFDTLVSSLKAVGLGDMPILVGEVGWPTDGDKNANAGNAYRFYNGLLPRLAANKGTP 300
           FDANFDTLVSSLKAVGLGDMPILVGEVGWPTDGDKNAN GNAYRFYNGLLPRLAANKGTP
Sbjct: 241 FDANFDTLVSSLKAVGLGDMPILVGEVGWPTDGDKNANDGNAYRFYNGLLPRLAANKGTP 300

Query: 301 LRPGFIEVYLFSFLDENGKSIAPGNFERHWGIFGYDGQPKFGMDLSGQGQNKLLVGAQNV 360
           LRPGFIEVYLFSFLDENGKSIAPGNFERHWGIFGYDGQPKFGMDLSGQGQNKLLVGAQNV
Sbjct: 301 LRPGFIEVYLFSFLDENGKSIAPGNFERHWGIFGYDGQPKFGMDLSGQGQNKLLVGAQNV 360

Query: 361 QYLPQKWCMFNPNAKDLSKLADNIDYACTFSDCTSLGYGSSCNNLDANGNASYAFNMYFQ 420
           +YLPQKWCMFNPNAKDLSKLADNIDYACTFSDCTSLGYGSSCNNLDANGNASYAFNMYFQ
Sbjct: 361 EYLPQKWCMFNPNAKDLSKLADNIDYACTFSDCTSLGYGSSCNNLDANGNASYAFNMYFQ 420

Query: 421 VQNQDELACNFQGLATITSQNLSQGTCNFIIQLGSSPPSHAASFVSLASMVLLFIALLL 462
           VQNQDELACNFQGLATITS+NLSQGTCNFIIQL SSPPSHAASFVSLASMVLLFIALLL
Sbjct: 421 VQNQDELACNFQGLATITSRNLSQGTCNFIIQLASSPPSHAASFVSLASMVLLFIALLL 479

BLAST of PI0008337 vs. NCBI nr
Match: XP_038903901.1 (glucan endo-1,3-beta-glucosidase 8-like [Benincasa hispida])

HSP 1 Score: 895.2 bits (2312), Expect = 2.4e-256
Identity = 443/479 (92.48%), Postives = 449/479 (93.74%), Query Frame = 0

Query: 1   MGRLGFLKWVLLVGVLGSCVEGLGVNWGTMANHRLPPKTIVQMFKDNGIQKVKLFDADQS 60
           MGRLGFLKWVLLVGVLG  VEGLGVNWGTMANHRLPPKTIVQMFKDNGI+KVKLFDADQS
Sbjct: 1   MGRLGFLKWVLLVGVLGCYVEGLGVNWGTMANHRLPPKTIVQMFKDNGIKKVKLFDADQS 60

Query: 61  SMGALAGTGIEVMVAIPNDQLSAMGDYNRAKQWVQRNVTRYIFDGGVTIKYVAVGNEPFL 120
           SMGALAGTGIEVMVAIPNDQLSAMGDYNRAKQWVQRNV+RY FDGGVTIKYVAVGNEPFL
Sbjct: 61  SMGALAGTGIEVMVAIPNDQLSAMGDYNRAKQWVQRNVSRYTFDGGVTIKYVAVGNEPFL 120

Query: 121 SSYNGSFLNVTFPALQNIQNALNEAGHGDSIKATVPLNADVYNSPVNSPYPSAGRFRNDI 180
           SSYNGSFLNVTFPALQNIQNALNEAGHGDSIKATVPLNADVYNSPVNSPYPSAGRFR+DI
Sbjct: 121 SSYNGSFLNVTFPALQNIQNALNEAGHGDSIKATVPLNADVYNSPVNSPYPSAGRFRSDI 180

Query: 181 NQLMTDIVH------------------LYGNDNFPFDYAFFDGASNPVVDIGTGIQYTNV 240
           N LMTDIV                   LYGNDNFPFDYAFFDGASNPVVD+GTGIQYTNV
Sbjct: 181 NGLMTDIVQFLSKNNAPFTVNIYPFLSLYGNDNFPFDYAFFDGASNPVVDVGTGIQYTNV 240

Query: 241 FDANFDTLVSSLKAVGLGDMPILVGEVGWPTDGDKNANAGNAYRFYNGLLPRLAANKGTP 300
           FDANFDTLVSSLKAV LGDMPILVGEVGWPTDGDKNANAGNAYRFYNGLLPRLAANKGTP
Sbjct: 241 FDANFDTLVSSLKAVELGDMPILVGEVGWPTDGDKNANAGNAYRFYNGLLPRLAANKGTP 300

Query: 301 LRPGFIEVYLFSFLDENGKSIAPGNFERHWGIFGYDGQPKFGMDLSGQGQNKLLVGAQNV 360
           LRPGFIEVYLFSFLDENGKSIAPGNFERHWGIFGYDGQPKF MDLSGQGQNKLLVGAQNV
Sbjct: 301 LRPGFIEVYLFSFLDENGKSIAPGNFERHWGIFGYDGQPKFAMDLSGQGQNKLLVGAQNV 360

Query: 361 QYLPQKWCMFNPNAKDLSKLADNIDYACTFSDCTSLGYGSSCNNLDANGNASYAFNMYFQ 420
           QYLP+KWCMFNPNAKDLSKLADNIDYACTFSDCTSLGYGSSCNNLDANGNASYAFNMYFQ
Sbjct: 361 QYLPKKWCMFNPNAKDLSKLADNIDYACTFSDCTSLGYGSSCNNLDANGNASYAFNMYFQ 420

Query: 421 VQNQDELACNFQGLATITSQNLSQGTCNFIIQLGSSPPSHAASFVSLASMVLLFIALLL 462
           VQNQDELACNFQGLAT+TSQNLSQGTCNFIIQL SSP S AASFVSLASM LLF ALLL
Sbjct: 421 VQNQDELACNFQGLATMTSQNLSQGTCNFIIQLASSPSSQAASFVSLASMALLFTALLL 479

BLAST of PI0008337 vs. NCBI nr
Match: XP_023526667.1 (glucan endo-1,3-beta-glucosidase 8-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 879.4 bits (2271), Expect = 1.4e-251
Identity = 434/479 (90.61%), Postives = 444/479 (92.69%), Query Frame = 0

Query: 1   MGRLGFLKWVLLVGVLGSCVEGLGVNWGTMANHRLPPKTIVQMFKDNGIQKVKLFDADQS 60
           MGRL  LKWVLLVGVLG CVEGLGVNWGTMANH+LPPKTIVQMFKDNGIQKVKLFDADQS
Sbjct: 1   MGRLWILKWVLLVGVLGCCVEGLGVNWGTMANHKLPPKTIVQMFKDNGIQKVKLFDADQS 60

Query: 61  SMGALAGTGIEVMVAIPNDQLSAMGDYNRAKQWVQRNVTRYIFDGGVTIKYVAVGNEPFL 120
           SMGALAGTGIEVMVAIPNDQL AMGDYNRAKQWVQRNV+RY FDGGVTIKYVAVGNEPFL
Sbjct: 61  SMGALAGTGIEVMVAIPNDQLVAMGDYNRAKQWVQRNVSRYTFDGGVTIKYVAVGNEPFL 120

Query: 121 SSYNGSFLNVTFPALQNIQNALNEAGHGDSIKATVPLNADVYNSPVNSPYPSAGRFRNDI 180
           SSYNGSFLNVTFPALQNIQNALNEAG+G++IKATVPLNADVYNSPVN+PYPSAGRFR+DI
Sbjct: 121 SSYNGSFLNVTFPALQNIQNALNEAGYGETIKATVPLNADVYNSPVNTPYPSAGRFRSDI 180

Query: 181 NQLMTDIVH------------------LYGNDNFPFDYAFFDGASNPVVDIGTGIQYTNV 240
           N LMT IV                   LYGNDNFPFDYAFFDGASNPVVD+ TGIQYTNV
Sbjct: 181 NGLMTQIVQFLSKNNAPFTVNIYPFLSLYGNDNFPFDYAFFDGASNPVVDMNTGIQYTNV 240

Query: 241 FDANFDTLVSSLKAVGLGDMPILVGEVGWPTDGDKNANAGNAYRFYNGLLPRLAANKGTP 300
           FDANFDTLVSSLKAVGLGDMPILVGEVGWPTDGDKNANAGNAYRFYNGLLPRLAANKGTP
Sbjct: 241 FDANFDTLVSSLKAVGLGDMPILVGEVGWPTDGDKNANAGNAYRFYNGLLPRLAANKGTP 300

Query: 301 LRPGFIEVYLFSFLDENGKSIAPGNFERHWGIFGYDGQPKFGMDLSGQGQNKLLVGAQNV 360
           LRPGFIEVYLFSFLDENGKSIAPGNFERHWGIFGYDGQPKFGMDLSGQGQNKLLVGAQ+V
Sbjct: 301 LRPGFIEVYLFSFLDENGKSIAPGNFERHWGIFGYDGQPKFGMDLSGQGQNKLLVGAQDV 360

Query: 361 QYLPQKWCMFNPNAKDLSKLADNIDYACTFSDCTSLGYGSSCNNLDANGNASYAFNMYFQ 420
           QYLPQKWCMFNPNAKDLSKLADNIDYACTFSDCTSLGYGSSCNNLDANGNASYAFNMYFQ
Sbjct: 361 QYLPQKWCMFNPNAKDLSKLADNIDYACTFSDCTSLGYGSSCNNLDANGNASYAFNMYFQ 420

Query: 421 VQNQDELACNFQGLATITSQNLSQGTCNFIIQLGSSPPSHAASFVSLASMVLLFIALLL 462
           VQNQDELACNFQGLATITSQNLSQGTC FIIQL SS PS  ASFVSL SM LLF A+LL
Sbjct: 421 VQNQDELACNFQGLATITSQNLSQGTCKFIIQLASSSPSFDASFVSLVSMALLFTAMLL 479

BLAST of PI0008337 vs. NCBI nr
Match: KAG7019320.1 (Glucan endo-1,3-beta-glucosidase 8 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 879.0 bits (2270), Expect = 1.8e-251
Identity = 433/479 (90.40%), Postives = 444/479 (92.69%), Query Frame = 0

Query: 1   MGRLGFLKWVLLVGVLGSCVEGLGVNWGTMANHRLPPKTIVQMFKDNGIQKVKLFDADQS 60
           MGRL  LKWVLLVGVLG C+EGLGVNWGTMANH+LPPKT+VQMFKDNGIQKVKLFDADQS
Sbjct: 1   MGRLWILKWVLLVGVLGCCIEGLGVNWGTMANHKLPPKTVVQMFKDNGIQKVKLFDADQS 60

Query: 61  SMGALAGTGIEVMVAIPNDQLSAMGDYNRAKQWVQRNVTRYIFDGGVTIKYVAVGNEPFL 120
           SMGALAGTGIEVMVAIPNDQL AMGDYNRAKQWVQRNV+RY FDGGVTIKYVAVGNEPFL
Sbjct: 61  SMGALAGTGIEVMVAIPNDQLVAMGDYNRAKQWVQRNVSRYTFDGGVTIKYVAVGNEPFL 120

Query: 121 SSYNGSFLNVTFPALQNIQNALNEAGHGDSIKATVPLNADVYNSPVNSPYPSAGRFRNDI 180
           SSYNGSFLNVTFPALQNIQNALNEAG+GD+IKATVPLNADVYNSPVN+PYPSAGRFR+DI
Sbjct: 121 SSYNGSFLNVTFPALQNIQNALNEAGYGDTIKATVPLNADVYNSPVNTPYPSAGRFRSDI 180

Query: 181 NQLMTDIVH------------------LYGNDNFPFDYAFFDGASNPVVDIGTGIQYTNV 240
           N LMT IV                   LYGNDNFPFDYAFFDGASNPVVD+ TGIQYTNV
Sbjct: 181 NGLMTQIVQFLSKNNAPFTVNIYPFLSLYGNDNFPFDYAFFDGASNPVVDMNTGIQYTNV 240

Query: 241 FDANFDTLVSSLKAVGLGDMPILVGEVGWPTDGDKNANAGNAYRFYNGLLPRLAANKGTP 300
           FDANFDTLVSSLKAVGLGDMPILVGEVGWPTDGDKNANAGNAYRFYNGLLPRLAANKGTP
Sbjct: 241 FDANFDTLVSSLKAVGLGDMPILVGEVGWPTDGDKNANAGNAYRFYNGLLPRLAANKGTP 300

Query: 301 LRPGFIEVYLFSFLDENGKSIAPGNFERHWGIFGYDGQPKFGMDLSGQGQNKLLVGAQNV 360
           LRPGFIEVYLFSFLDENGKSIAPGNFERHWGIFGYDGQPKFGMDLSGQGQNKLLVGAQ+V
Sbjct: 301 LRPGFIEVYLFSFLDENGKSIAPGNFERHWGIFGYDGQPKFGMDLSGQGQNKLLVGAQDV 360

Query: 361 QYLPQKWCMFNPNAKDLSKLADNIDYACTFSDCTSLGYGSSCNNLDANGNASYAFNMYFQ 420
           QYLPQKWCMFNPNAKDLSKLADNIDYACTFSDCTSLGYGSSCNNLDANGNASYAFNMYFQ
Sbjct: 361 QYLPQKWCMFNPNAKDLSKLADNIDYACTFSDCTSLGYGSSCNNLDANGNASYAFNMYFQ 420

Query: 421 VQNQDELACNFQGLATITSQNLSQGTCNFIIQLGSSPPSHAASFVSLASMVLLFIALLL 462
           VQNQDELACNFQGLATITSQNLSQGTC FIIQL SS PS  ASFVSL SM LLFIA+ L
Sbjct: 421 VQNQDELACNFQGLATITSQNLSQGTCKFIIQLASSSPSLDASFVSLVSMALLFIAMWL 479

BLAST of PI0008337 vs. TAIR 10
Match: AT1G64760.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 680.6 bits (1755), Expect = 8.6e-196
Identity = 336/468 (71.79%), Postives = 383/468 (81.84%), Query Frame = 0

Query: 9   WVLLVGVLGSCVEGLGVNWGTMANHRLPPKTIVQMFKDNGIQKVKLFDADQSSMGALAGT 68
           +V+++G LG  V GLGVNWGTMA H+LPPKT+VQM KDN I KVKLFDAD+++MGALAG+
Sbjct: 11  FVIVIGHLGILVNGLGVNWGTMATHKLPPKTVVQMLKDNNINKVKLFDADETTMGALAGS 70

Query: 69  GIEVMVAIPNDQLSAMGDYNRAKQWVQRNVTRYIFDGGVTIKYVAVGNEPFLSSYNGSFL 128
           G+EVMVAIPNDQL  M  Y+RAK WV++NVTRY FDGGV I +VAVGNEPFL SYNGSF+
Sbjct: 71  GLEVMVAIPNDQLKVMTSYDRAKDWVRKNVTRYNFDGGVNITFVAVGNEPFLKSYNGSFI 130

Query: 129 NVTFPALQNIQNALNEAGHGDSIKATVPLNADVYNSPVNSPYPSAGRFRNDINQLMTDIV 188
           N+TFPAL NIQNALNEAG G+S+KATVPLNADVY+SP ++P PSAGRFR DI   MT IV
Sbjct: 131 NLTFPALANIQNALNEAGLGNSVKATVPLNADVYDSPASNPVPSAGRFRPDIIGQMTQIV 190

Query: 189 ------------------HLYGNDNFPFDYAFFDGASNPVVDIGTGIQYTNVFDANFDTL 248
                              LYGND+FP +YAFFDGA  P+ D   GI YTNVFDANFDTL
Sbjct: 191 DFLGKNNAPITINIYPFLSLYGNDDFPLNYAFFDGA-EPIND--NGIDYTNVFDANFDTL 250

Query: 249 VSSLKAVGLGDMPILVGEVGWPTDGDKNANAGNAYRFYNGLLPRLAANKGTPLRPGFIEV 308
           VSSLKAVG GDMPI+VGEVGWPT+GDK+ANAG+AYRFYNGLLPRL  NKGTPLRP +IEV
Sbjct: 251 VSSLKAVGHGDMPIIVGEVGWPTEGDKHANAGSAYRFYNGLLPRLGTNKGTPLRPTYIEV 310

Query: 309 YLFSFLDENGKSIAPGNFERHWGIFGYDGQPKFGMDLSGQGQNKLLVGAQNVQYLPQKWC 368
           YLF  LDE+ KSIAPG FERHWGIF +DGQPKF +DLSGQGQ+K L+GAQNV YLP KWC
Sbjct: 311 YLFGLLDEDAKSIAPGPFERHWGIFKFDGQPKFPIDLSGQGQSKFLIGAQNVPYLPNKWC 370

Query: 369 MFNPNAKDLSKLADNIDYACTFSDCTSLGYGSSCNNLDANGNASYAFNMYFQVQNQDELA 428
            FNP AKDL+KLA NIDYACTFSDCT+LGYGSSCN LDANGNASYAFNM+FQV+NQDE A
Sbjct: 371 TFNPEAKDLTKLAANIDYACTFSDCTALGYGSSCNTLDANGNASYAFNMFFQVKNQDESA 430

Query: 429 CNFQGLATITSQNLSQGTCNFIIQL-GSSPPSHAASFVSLASMVLLFI 458
           C FQGLATIT+QN+SQG CNF IQ+  SS  S + S  SL  +++ F+
Sbjct: 431 CYFQGLATITTQNISQGQCNFPIQIVASSASSFSCSSYSLVVLIVWFL 475

BLAST of PI0008337 vs. TAIR 10
Match: AT1G64760.2 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 680.6 bits (1755), Expect = 8.6e-196
Identity = 336/468 (71.79%), Postives = 383/468 (81.84%), Query Frame = 0

Query: 9   WVLLVGVLGSCVEGLGVNWGTMANHRLPPKTIVQMFKDNGIQKVKLFDADQSSMGALAGT 68
           +V+++G LG  V GLGVNWGTMA H+LPPKT+VQM KDN I KVKLFDAD+++MGALAG+
Sbjct: 11  FVIVIGHLGILVNGLGVNWGTMATHKLPPKTVVQMLKDNNINKVKLFDADETTMGALAGS 70

Query: 69  GIEVMVAIPNDQLSAMGDYNRAKQWVQRNVTRYIFDGGVTIKYVAVGNEPFLSSYNGSFL 128
           G+EVMVAIPNDQL  M  Y+RAK WV++NVTRY FDGGV I +VAVGNEPFL SYNGSF+
Sbjct: 71  GLEVMVAIPNDQLKVMTSYDRAKDWVRKNVTRYNFDGGVNITFVAVGNEPFLKSYNGSFI 130

Query: 129 NVTFPALQNIQNALNEAGHGDSIKATVPLNADVYNSPVNSPYPSAGRFRNDINQLMTDIV 188
           N+TFPAL NIQNALNEAG G+S+KATVPLNADVY+SP ++P PSAGRFR DI   MT IV
Sbjct: 131 NLTFPALANIQNALNEAGLGNSVKATVPLNADVYDSPASNPVPSAGRFRPDIIGQMTQIV 190

Query: 189 ------------------HLYGNDNFPFDYAFFDGASNPVVDIGTGIQYTNVFDANFDTL 248
                              LYGND+FP +YAFFDGA  P+ D   GI YTNVFDANFDTL
Sbjct: 191 DFLGKNNAPITINIYPFLSLYGNDDFPLNYAFFDGA-EPIND--NGIDYTNVFDANFDTL 250

Query: 249 VSSLKAVGLGDMPILVGEVGWPTDGDKNANAGNAYRFYNGLLPRLAANKGTPLRPGFIEV 308
           VSSLKAVG GDMPI+VGEVGWPT+GDK+ANAG+AYRFYNGLLPRL  NKGTPLRP +IEV
Sbjct: 251 VSSLKAVGHGDMPIIVGEVGWPTEGDKHANAGSAYRFYNGLLPRLGTNKGTPLRPTYIEV 310

Query: 309 YLFSFLDENGKSIAPGNFERHWGIFGYDGQPKFGMDLSGQGQNKLLVGAQNVQYLPQKWC 368
           YLF  LDE+ KSIAPG FERHWGIF +DGQPKF +DLSGQGQ+K L+GAQNV YLP KWC
Sbjct: 311 YLFGLLDEDAKSIAPGPFERHWGIFKFDGQPKFPIDLSGQGQSKFLIGAQNVPYLPNKWC 370

Query: 369 MFNPNAKDLSKLADNIDYACTFSDCTSLGYGSSCNNLDANGNASYAFNMYFQVQNQDELA 428
            FNP AKDL+KLA NIDYACTFSDCT+LGYGSSCN LDANGNASYAFNM+FQV+NQDE A
Sbjct: 371 TFNPEAKDLTKLAANIDYACTFSDCTALGYGSSCNTLDANGNASYAFNMFFQVKNQDESA 430

Query: 429 CNFQGLATITSQNLSQGTCNFIIQL-GSSPPSHAASFVSLASMVLLFI 458
           C FQGLATIT+QN+SQG CNF IQ+  SS  S + S  SL  +++ F+
Sbjct: 431 CYFQGLATITTQNISQGQCNFPIQIVASSASSFSCSSYSLVVLIVWFL 475

BLAST of PI0008337 vs. TAIR 10
Match: AT2G19440.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 665.6 bits (1716), Expect = 2.9e-191
Identity = 334/471 (70.91%), Postives = 384/471 (81.53%), Query Frame = 0

Query: 4   LGFLKWVLLVGVLG-SCVEGLGVNWGTMANHRLPPKTIVQMFKDNGIQKVKLFDADQSSM 63
           +  L +V+  G++G   V+GLGVNWGTMA H+LPPK +VQM KDN I KVKLFDAD+++M
Sbjct: 1   MNLLAFVVGFGIMGIVMVDGLGVNWGTMATHKLPPKKVVQMLKDNNINKVKLFDADETTM 60

Query: 64  GALAGTGIEVMVAIPNDQLSAMGDYNRAKQWVQRNVTRYIFDGGVTIKYVAVGNEPFLSS 123
            AL+G+G+EVMVAIPNDQL  MG Y+RAK WV +NVTRY F+GGV I +VAVGNEPFL S
Sbjct: 61  SALSGSGLEVMVAIPNDQLKVMGSYDRAKDWVHKNVTRYNFNGGVNITFVAVGNEPFLKS 120

Query: 124 YNGSFLNVTFPALQNIQNALNEAGHGDSIKATVPLNADVYNSPVNSPYPSAGRFRNDINQ 183
           YNGSF+N+TFPALQNIQNALNEAG G S+KATVPLNADVY+SP ++P PSAGRFR DI  
Sbjct: 121 YNGSFINLTFPALQNIQNALNEAGLGSSVKATVPLNADVYDSPSSNPVPSAGRFRPDIIG 180

Query: 184 LMTDIV------------------HLYGNDNFPFDYAFFDGASNPVVDIGTGIQYTNVFD 243
            MT IV                   LYGND+FP +YAFFDGA  PV D   GI YTNVFD
Sbjct: 181 QMTQIVDFLGNNSAPITINIYPFLSLYGNDDFPLNYAFFDGA-KPVDD--NGIAYTNVFD 240

Query: 244 ANFDTLVSSLKAVGLGDMPILVGEVGWPTDGDKNANAGNAYRFYNGLLPRLAANKGTPLR 303
           ANFDTLVS+LKAVG GDMPI+VGEVGWPT+GDK+AN+G+AYRFYNGLLPRL  N+GTPLR
Sbjct: 241 ANFDTLVSALKAVGHGDMPIIVGEVGWPTEGDKHANSGSAYRFYNGLLPRLGENRGTPLR 300

Query: 304 PGFIEVYLFSFLDENGKSIAPGNFERHWGIFGYDGQPKFGMDLSGQGQNKLLVGAQNVQY 363
           P +IEVYLF  LDE+ KSIAPG FERHWGIF +DGQPKF +DLSGQGQNKLL+GA+NV Y
Sbjct: 301 PTYIEVYLFGLLDEDAKSIAPGEFERHWGIFKFDGQPKFPIDLSGQGQNKLLIGAENVTY 360

Query: 364 LPQKWCMFNPNAKDLSKLADNIDYACTFSDCTSLGYGSSCNNLDANGNASYAFNMYFQVQ 423
            P+KWCMFN  AKDL+KLA NIDYACTFSDCT+LGYGSSCN LDANGNASYAFNMYFQV+
Sbjct: 361 QPKKWCMFNTEAKDLTKLAANIDYACTFSDCTALGYGSSCNTLDANGNASYAFNMYFQVK 420

Query: 424 NQDELACNFQGLATITSQNLSQGTCNFIIQLGSSPPSHAASFVSLASMVLL 456
           NQDE AC FQGLATIT++N+SQG CNF IQ+ +S    A+SF S  S+VLL
Sbjct: 421 NQDEDACIFQGLATITTKNISQGQCNFPIQIVAST---ASSFSS--SLVLL 463

BLAST of PI0008337 vs. TAIR 10
Match: AT3G04010.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 620.5 bits (1599), Expect = 1.1e-177
Identity = 309/463 (66.74%), Postives = 365/463 (78.83%), Query Frame = 0

Query: 16  LGSCVEGLGVNWGTMANHRLPPKTIVQMFKDNGIQKVKLFDADQSSMGALAGTGIEVMVA 75
           L   V GLGVNWGTMA+H+LPPKT+V+M KDN IQKVKLFDAD ++MGALAG+G+EVMVA
Sbjct: 26  LSGGVSGLGVNWGTMASHQLPPKTVVEMLKDNNIQKVKLFDADTNTMGALAGSGVEVMVA 85

Query: 76  IPNDQLSAMGDYNRAKQWVQRNVTRYIFDGGVTIKYVAVGNEPFLSSYNGSFLNVTFPAL 135
           IPND L AMG+Y RAK WVQRNV+R+ F+ GV IKYVAVGNEPFL++YNGSF+N+T+PAL
Sbjct: 86  IPNDLLLAMGNYQRAKDWVQRNVSRFNFNNGVKIKYVAVGNEPFLTAYNGSFINLTYPAL 145

Query: 136 QNIQNALNEAGHGDSIKATVPLNADVYNSPVNSPYPSAGRFRNDINQLMTDIVH------ 195
            NIQ ALNEAG GD  KATVPLNADVYNSP ++  PSAGRFR+DI Q MT IV+      
Sbjct: 146 FNIQTALNEAGVGDFTKATVPLNADVYNSPPDNQVPSAGRFRSDIIQEMTQIVNFLAQNK 205

Query: 196 ------------LYGNDNFPFDYAFFDGASNPVVDIGTGIQYTNVFDANFDTLVSSLKAV 255
                       LY + +FPF+YAFFDG  N V D   G+ YTNVFDA+FDTL++SL A+
Sbjct: 206 APFTVNIYPFLSLYLSSDFPFEYAFFDG-QNTVND--NGVIYTNVFDASFDTLLASLNAL 265

Query: 256 GLGDMPILVGEVGWPTDGDKNANAGNAYRFYNGLLPRLAANKGTPLRPGFIEVYLFSFLD 315
             G+M ++VGEVGWPTDGDKNAN  NA RFY+GLLPRLA N GTP+R G+IEVYLF F+D
Sbjct: 266 NHGNMEVIVGEVGWPTDGDKNANVPNAERFYSGLLPRLANNVGTPMRKGYIEVYLFGFID 325

Query: 316 ENGKSIAPGNFERHWGIFGYDGQPKFGMDLSGQGQNKLLVGAQNVQYLPQKWCMFNPNAK 375
           E+ KS+APGNFERHWGIF +DGQPKF +D  GQGQ K L GAQNVQY   +WCMFNPN +
Sbjct: 326 EDAKSVAPGNFERHWGIFKFDGQPKFPVDFRGQGQKKFLTGAQNVQYFLNQWCMFNPNGR 385

Query: 376 -DLSKLADNIDYACTFSDCTSLGYGSSCNNLDANGNASYAFNMYFQVQNQDELACNFQGL 435
            ++S+L DNI+YAC+ SDCT+LGYGSSC NLDANGNASYAFNMYFQVQNQ+  AC+F+GL
Sbjct: 386 GNMSRLGDNINYACSHSDCTALGYGSSCGNLDANGNASYAFNMYFQVQNQEAQACDFEGL 445

Query: 436 ATITSQNLSQGTCNFIIQLGSSPPSHAASFVSLASMVLLFIAL 460
           ATIT+QN+SQG CNF IQ+G   PS      S  SMV L + +
Sbjct: 446 ATITTQNISQGQCNFPIQIGE--PSSGHYDYSYGSMVRLCLVM 483

BLAST of PI0008337 vs. TAIR 10
Match: AT5G18220.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 620.5 bits (1599), Expect = 1.1e-177
Identity = 315/482 (65.35%), Postives = 370/482 (76.76%), Query Frame = 0

Query: 5   GFLKWVLLVGVLGSCVEGLGVNWGTMANHRLPPKTIVQMFKDNGIQKVKLFDADQSSMGA 64
           G    + +V +L   V  LGVNWGTM+ H+LPPKT+VQM KDN ++KVKLFDAD ++M A
Sbjct: 12  GIFLCITIVSLLSGDVSALGVNWGTMSTHQLPPKTVVQMLKDNNVKKVKLFDADTNTMVA 71

Query: 65  LAGTGIEVMVAIPNDQLSAMGDYNRAKQWVQRNVTRYIFDGGVTIKYVAVGNEPFLSSYN 124
           LAG+GIEVMVAIPNDQL AMG YNRAK WV+RN+TR  F+  V IKYVAVGNEPFL++YN
Sbjct: 72  LAGSGIEVMVAIPNDQLKAMGSYNRAKDWVRRNITR--FNDDVKIKYVAVGNEPFLTAYN 131

Query: 125 GSFLNVTFPALQNIQNALNEAGHGDSIKATVPLNADVYNSPVNSPYPSAGRFRNDINQLM 184
           GSF+N+T+PAL NIQ ALNEAG GD IKATVPLNADVYNSP+ +P PSAG FR DI + M
Sbjct: 132 GSFINLTYPALFNIQKALNEAGVGDFIKATVPLNADVYNSPLENPVPSAGSFRQDIFEEM 191

Query: 185 TDIVH------------------LYGNDNFPFDYAFFDGASNPVVDIGTGIQYTNVFDAN 244
             IV+                  LY + +FPFDYAFF+G  N V D   G+ YTNVFDAN
Sbjct: 192 KLIVNFLAHNKAPFTVNIYPFLSLYLSSDFPFDYAFFNG-QNTVSD--NGVIYTNVFDAN 251

Query: 245 FDTLVSSLKAVGLGDMPILVGEVGWPTDGDKNANAGNAYRFYNGLLPRLAANKGTPLRPG 304
           FDTL++SLKA+G GDM ++VGEVGWPTDGDKNAN  NA RFY GLLP+LAAN+GTP+RPG
Sbjct: 252 FDTLLASLKALGHGDMTVIVGEVGWPTDGDKNANIPNAERFYTGLLPKLAANRGTPMRPG 311

Query: 305 FIEVYLFSFLDENGKSIAPGNFERHWGIFGYDGQPKFGMDLSGQGQNKLLVGAQNVQYLP 364
           +IEVYLF F+DE+ KSIAPGNFERHWGIF YDGQPKF  DLSG GQ K+L GAQNVQYL 
Sbjct: 312 YIEVYLFGFIDEDAKSIAPGNFERHWGIFKYDGQPKFPADLSGAGQKKILTGAQNVQYLR 371

Query: 365 QKWCMFNPNA----KDLSKLADNIDYACTFSDCTSLGYGSSCNNLDANGNASYAFNMYFQ 424
            +WCMFNPNA     + ++L DN++YACTFSDCT+LGYGSSC NLD  GNASYAFNMYFQ
Sbjct: 372 NQWCMFNPNALTFSNNTNQLGDNVNYACTFSDCTALGYGSSCGNLDEVGNASYAFNMYFQ 431

Query: 425 VQNQDELACNFQGLATITSQNLSQGTCNFIIQLGS-----SPPSHAASFVSLASMVLLFI 460
           VQNQ   AC+F+GLA IT++N+S+  CNF IQ+G      S  +H     SL SM+LLF 
Sbjct: 432 VQNQKAEACDFEGLAIITTRNISREQCNFPIQIGDPTSGHSDHNHRFISFSLVSMLLLFT 488

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q6NKW91.2e-19471.79Glucan endo-1,3-beta-glucosidase 8 OS=Arabidopsis thaliana OX=3702 GN=At1g64760 ... [more]
Q9M0885.9e-12550.32Glucan endo-1,3-beta-glucosidase 5 OS=Arabidopsis thaliana OX=3702 GN=At4g31140 ... [more]
Q93Z081.0e-12148.78Glucan endo-1,3-beta-glucosidase 6 OS=Arabidopsis thaliana OX=3702 GN=At5g58090 ... [more]
Q9FGH43.7e-8739.83Glucan endo-1,3-beta-glucosidase 9 OS=Arabidopsis thaliana OX=3702 GN=At5g58480 ... [more]
Q94G869.6e-5132.18Glucan endo-1,3-beta-D-glucosidase OS=Olea europaea OX=4146 GN=OLE9 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0LFN21.7e-26394.15(1->3)-beta-glucan endohydrolase OS=Cucumis sativus OX=3659 GN=Csa_3G843830 PE=3... [more]
A0A5D3E2812.2e-26394.36(1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676... [more]
A0A1S3CNJ02.2e-26394.36(1->3)-beta-glucan endohydrolase OS=Cucumis melo OX=3656 GN=LOC103502975 PE=3 SV... [more]
A0A6J1I9Q32.5e-25189.98(1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111472848 PE=... [more]
A0A6J1EXE02.7e-25089.94(1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111439258 P... [more]
Match NameE-valueIdentityDescription
XP_004141142.13.4e-26394.15glucan endo-1,3-beta-glucosidase 8 [Cucumis sativus] >KGN59764.1 hypothetical pr... [more]
XP_008465328.14.5e-26394.36PREDICTED: glucan endo-1,3-beta-glucosidase 8-like [Cucumis melo] >KAA0051277.1 ... [more]
XP_038903901.12.4e-25692.48glucan endo-1,3-beta-glucosidase 8-like [Benincasa hispida][more]
XP_023526667.11.4e-25190.61glucan endo-1,3-beta-glucosidase 8-like isoform X1 [Cucurbita pepo subsp. pepo][more]
KAG7019320.11.8e-25190.40Glucan endo-1,3-beta-glucosidase 8 [Cucurbita argyrosperma subsp. argyrosperma][more]
Match NameE-valueIdentityDescription
AT1G64760.18.6e-19671.79O-Glycosyl hydrolases family 17 protein [more]
AT1G64760.28.6e-19671.79O-Glycosyl hydrolases family 17 protein [more]
AT2G19440.12.9e-19170.91O-Glycosyl hydrolases family 17 protein [more]
AT3G04010.11.1e-17766.74O-Glycosyl hydrolases family 17 protein [more]
AT5G18220.11.1e-17765.35O-Glycosyl hydrolases family 17 protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR012946X8 domainSMARTSM00768X8_clscoord: 348..431
e-value: 7.6E-38
score: 141.7
IPR012946X8 domainPFAMPF07983X8coord: 348..418
e-value: 8.8E-18
score: 64.8
NoneNo IPR availableGENE3D3.20.20.80Glycosidasescoord: 22..334
e-value: 1.6E-91
score: 308.6
NoneNo IPR availableGENE3D1.20.58.1040coord: 345..438
e-value: 8.2E-9
score: 37.7
NoneNo IPR availablePANTHERPTHR32227:SF95GLUCAN ENDO-1,3-BETA-GLUCOSIDASE 8-RELATEDcoord: 8..438
NoneNo IPR availablePROSITEPS51257PROKAR_LIPOPROTEINcoord: 1..19
score: 5.0
IPR000490Glycoside hydrolase family 17PFAMPF00332Glyco_hydro_17coord: 23..326
e-value: 5.4E-52
score: 177.3
IPR000490Glycoside hydrolase family 17PROSITEPS00587GLYCOSYL_HYDROL_F17coord: 242..255
IPR044965Glycoside hydrolase family 17, plantPANTHERPTHR32227GLUCAN ENDO-1,3-BETA-GLUCOSIDASE BG1-RELATED-RELATEDcoord: 8..438
IPR017853Glycoside hydrolase superfamilySUPERFAMILY51445(Trans)glycosidasescoord: 23..326

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0008337.1PI0008337.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
cellular_component GO:0046658 anchored component of plasma membrane
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0042973 glucan endo-1,3-beta-D-glucosidase activity
molecular_function GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds