PI0006323 (gene) Melon (PI 482460) v1

Overview
NamePI0006323
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
DescriptionGDSL esterase/lipase
Locationchr08: 17226731 .. 17228750 (+)
RNA-Seq ExpressionPI0006323
SyntenyPI0006323
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCAGCTTCGAGTCATTGGCTTCTTGCTTCCCTTCACCCTTCTAGCTCTCATTTTTCCTGTGGCTATCAGTGATCCTCTTGTCCCTGCACTCTGCATCTTCGGCGACTCGGTGTCGATGTTGGAAATAACAACAATCTTCTAACTGTTGTCAAAGCTAACTTCCCTCCTATGGAAGAGACTTTGTTACTCACGCACCAACAGGAAGGTTTTGCAATGGCAAGCTTGCCACTGATTTTACTGGTATCTTTCCATCTTCTTCTTTATTTTTCTCCAAAGTCCCGTTTTCATCCTTTCCGACCAGTTTGTTTCTGTTTTTTATTATATACACAGCTGAACTCCTTGGATTTTCTTCATATCCTCCTGCGTATTTGAGCCAAGATGCTACTGGAAACAAGCTTTAACAGGAGCTAACTTTGCTTCTGCTGCTTCTGGCTTTTATGATGGCACAGCTCAGCTCTATGTAAATAATCTTCTATAACTCTCCATGCAGTAGTTTATGAACTGTGTACTAACATCGAAGCTAACACTTGAAATAAGGTGTTTAGATCTAATATCTGATTGAGATCGTGTGTACGAACTCATCAGTGTTGGCTTTCGTTTTAAGTTTTTTAGTTTAGTGACATTTAATATGGCCATGTTTGCGTTGGTTTTGCAGCATGCAGTTTCACTAACTCAGCAGTTGAATTATTACAAGGAATATCAAAGCAAAGTTGTAAACATGGTGGGAACAGAGAAAGCCAATGCTATATTCTCTGGTGCAATTCATCTTCTTAGTGCAGGCAGCAGTGATTTCATTCAGAATACTATGTTAATCCTCTTCTTTACAGAACATACTCACCACAGCAGTTCTCTGACATTCTCATTACATCTTCTCAAACTTCATTCAGGTTTCCCAGTTTTTTTATTTTGATTTCTTCTTATTTACCGATATATAATGCATCTTGAGGAACTGAGAACAGATTCCCACAGTTTCTCTTTCACAAACCTCAAATTTATTTTCATATTTTTTGACTACCTTATGTTGGCTAAATTACTATATATCAAGAATTCTTTGAGCCCCATGAATTTAAACCATGATGTACAACCATTGAAACAAGTTTTTTGTCTGAGTGTTTTTAACATCAAATACTACAAACATATCAAGATCTTCACTTTGACAGAACTTATATGGAATGGGAGCAAGGAGAATAGGGGTGACGGGATTACCTCCATTGGGTTGCTTGCCAGCAGCTATAACCCTCTTTGGTTCTGGCAGCAATCAGTGCATCCAAAGGTTAAATCAAGATGCCATTGCATTCAACACTAAGCTGCAAAGCGCAACTAAGAGTTTGCAGAACAGATTCTCTGATCTCAAGCTTGTTGCCTTTGATATTTATCAACCTCTCTTGAATATGGTCTCAAAGCCTGCAGAAAATGGTAAAAATCCTCCTTAAAACAGCTTCACGTGGAACTTTAAATTAAAGGATATGCATATGAAAACATTCTCAAAGTTTTGCATACCCCTTTACGTTATTGCCTACTGATCTGTTTTGAGATCATTCTGCATAGTTGATGGAGATTTACAATATGCACAAATTTGTTTCCCAATTAGTTTTGTTTGCTTCTCTTTGCTCTTTGCTCTTTGAGCCCTTGAGAAACCTCTCAACCAGTTTTGAAAGACGAGTTTTACGCTCAGTTCAAGATGGTTTTCCCTCTCTTTCTCAAATTGTATTATTTCCATCACACCTTACAATTTTCATCAGAGATAAAAGTGCAGATGGAAAGATGAAAAAGACTTTTTTTTTAGATGGAAACTGACCTTTCTAGTTGGAACTTTGAATGTCACAGGGTTTTTCGAGTCGAGAAGGGCTTGTTGTGGAACAGGAACAGTAGAGACCTCCTTCCTTTGCAACAGTAGATCAGTTGGAACCTGCTCAAATGCCACCGGCTACGTTTTCTGGGACGGTTTCCATCCAACTGAAGCTGCAAATCAAGTTTTGGCTGGGGATTTACTTACTCAGGGATTCAGCCTCATCTGA

mRNA sequence

ATGCAGCTTCGAGTCATTGGCTTCTTGCTTCCCTTCACCCTTCTAGCTCTCATTTTTCCTGTGGCTATCAGTGATCCTCTTGTCCCTGCACTCTGCATCTTCGGCGACTCGGTGTCGATAGACTTTGTTACTCACGCACCAACAGGAAGGTTTTGCAATGGCAAGCTTGCCACTGATTTTACTGCTTTAACAGGAGCTAACTTTGCTTCTGCTGCTTCTGGCTTTTATGATGGCACAGCTCAGCTCTATCATGCAGTTTCACTAACTCAGCAGTTGAATTATTACAAGGAATATCAAAGCAAAGTTGTAAACATGGTGGGAACAGAGAAAGCCAATGCTATATTCTCTGGTGCAATTCATCTTCTTAGTGCAGGCAGCAGTGATTTCATTCAGAATACTATGTTAATCCTCTTCTTTACAGAACATACTCACCACAGCAGTTCTCTGACATTCTCATTACATCTTCTCAAACTTCATTCAGGTTTCCCAAACTTATATGGAATGGGAGCAAGGAGAATAGGGGTGACGGGATTACCTCCATTGGGTTGCTTGCCAGCAGCTATAACCCTCTTTGGTTCTGGCAGCAATCAGTGCATCCAAAGGTTAAATCAAGATGCCATTGCATTCAACACTAAGCTGCAAAGCGCAACTAAGAGTTTGCAGAACAGATTCTCTGATCTCAAGCTTGTTGCCTTTGATATTTATCAACCTCTCTTGAATATGGTCTCAAAGCCTGCAGAAAATGGGTTTTTCGAGTCGAGAAGGGCTTGTTGTGGAACAGGAACAGTAGAGACCTCCTTCCTTTGCAACAGTAGATCAGTTGGAACCTGCTCAAATGCCACCGGCTACGTTTTCTGGGACGGTTTCCATCCAACTGAAGCTGCAAATCAAGTTTTGGCTGGGGATTTACTTACTCAGGGATTCAGCCTCATCTGA

Coding sequence (CDS)

ATGCAGCTTCGAGTCATTGGCTTCTTGCTTCCCTTCACCCTTCTAGCTCTCATTTTTCCTGTGGCTATCAGTGATCCTCTTGTCCCTGCACTCTGCATCTTCGGCGACTCGGTGTCGATAGACTTTGTTACTCACGCACCAACAGGAAGGTTTTGCAATGGCAAGCTTGCCACTGATTTTACTGCTTTAACAGGAGCTAACTTTGCTTCTGCTGCTTCTGGCTTTTATGATGGCACAGCTCAGCTCTATCATGCAGTTTCACTAACTCAGCAGTTGAATTATTACAAGGAATATCAAAGCAAAGTTGTAAACATGGTGGGAACAGAGAAAGCCAATGCTATATTCTCTGGTGCAATTCATCTTCTTAGTGCAGGCAGCAGTGATTTCATTCAGAATACTATGTTAATCCTCTTCTTTACAGAACATACTCACCACAGCAGTTCTCTGACATTCTCATTACATCTTCTCAAACTTCATTCAGGTTTCCCAAACTTATATGGAATGGGAGCAAGGAGAATAGGGGTGACGGGATTACCTCCATTGGGTTGCTTGCCAGCAGCTATAACCCTCTTTGGTTCTGGCAGCAATCAGTGCATCCAAAGGTTAAATCAAGATGCCATTGCATTCAACACTAAGCTGCAAAGCGCAACTAAGAGTTTGCAGAACAGATTCTCTGATCTCAAGCTTGTTGCCTTTGATATTTATCAACCTCTCTTGAATATGGTCTCAAAGCCTGCAGAAAATGGGTTTTTCGAGTCGAGAAGGGCTTGTTGTGGAACAGGAACAGTAGAGACCTCCTTCCTTTGCAACAGTAGATCAGTTGGAACCTGCTCAAATGCCACCGGCTACGTTTTCTGGGACGGTTTCCATCCAACTGAAGCTGCAAATCAAGTTTTGGCTGGGGATTTACTTACTCAGGGATTCAGCCTCATCTGA

Protein sequence

MQLRVIGFLLPFTLLALIFPVAISDPLVPALCIFGDSVSIDFVTHAPTGRFCNGKLATDFTALTGANFASAASGFYDGTAQLYHAVSLTQQLNYYKEYQSKVVNMVGTEKANAIFSGAIHLLSAGSSDFIQNTMLILFFTEHTHHSSSLTFSLHLLKLHSGFPNLYGMGARRIGVTGLPPLGCLPAAITLFGSGSNQCIQRLNQDAIAFNTKLQSATKSLQNRFSDLKLVAFDIYQPLLNMVSKPAENGFFESRRACCGTGTVETSFLCNSRSVGTCSNATGYVFWDGFHPTEAANQVLAGDLLTQGFSLI
Homology
BLAST of PI0006323 vs. ExPASy Swiss-Prot
Match: Q9FFN0 (GDSL esterase/lipase At5g03810 OS=Arabidopsis thaliana OX=3702 GN=At5g03810 PE=3 SV=1)

HSP 1 Score: 351.7 bits (901), Expect = 8.7e-96
Identity = 199/339 (58.70%), Postives = 228/339 (67.26%), Query Frame = 0

Query: 21  VAISDPLVPALCIFGDSV--------------------SIDFVTHAPTGRFCNGKLATDF 80
           V   + LVPAL I GDSV                      DFV H+ TGRF NGKLATDF
Sbjct: 21  VGTGETLVPALIIMGDSVVDAGNNNHRITLVKANFPPYGRDFVAHSATGRFSNGKLATDF 80

Query: 81  TA----------------------LTGANFASAASGFYDGTAQLYHAVSLTQQLNYYKEY 140
           TA                      LTGANFAS ASGF D TA  Y+A++L+QQL  YKEY
Sbjct: 81  TAENLGFTSYPVAYLSQEANETNLLTGANFASGASGFDDATAIFYNAITLSQQLKNYKEY 140

Query: 141 QSKVVNMVGTEKANAIFSGAIHLLSAGSSDFIQ----NTMLILFFTEHTHHSSSLTFSLH 200
           Q+KV N+VG E+AN IFSGAIHLLS GSSDF+Q    N +L   FT          +S H
Sbjct: 141 QNKVTNIVGKERANEIFSGAIHLLSTGSSDFLQSYYINPILNRIFTPD-------QYSDH 200

Query: 201 LLKLHSGF-PNLYGMGARRIGVTGLPPLGCLPAAITLFGS-GSNQCIQRLNQDAIAFNTK 260
           LL+ +S F  NLYG+GARRIGVT LPPLGCLPAAITLFG  G+N C++RLNQDA++FNTK
Sbjct: 201 LLRSYSTFVQNLYGLGARRIGVTTLPPLGCLPAAITLFGGVGNNMCVERLNQDAVSFNTK 260

Query: 261 LQSATKSLQNRFSDLKLVAFDIYQPLLNMVSKPAENGFFESRRACCGTGTVETSFLCNSR 312
           L + + +L N    LKLV FDIY PLLNMV  P E GFFESRRACCGTGT+ETSFLCN+ 
Sbjct: 261 LNNTSINLTNNLPGLKLVVFDIYNPLLNMVINPVEYGFFESRRACCGTGTMETSFLCNAL 320

BLAST of PI0006323 vs. ExPASy Swiss-Prot
Match: Q9LZC5 (GDSL esterase/lipase At5g03820 OS=Arabidopsis thaliana OX=3702 GN=At5g03820 PE=3 SV=1)

HSP 1 Score: 350.5 bits (898), Expect = 1.9e-95
Identity = 200/353 (56.66%), Postives = 235/353 (66.57%), Query Frame = 0

Query: 9   LLPFTLLALIFP-VAISDPLVPALCIFGDSV--------------------SIDFVTHAP 68
           L+ F+++A  +  V   +PLVPAL I GDSV                      DF+ H  
Sbjct: 8   LMTFSVIACFYAGVGTGEPLVPALIIMGDSVVDAGNNNRLNTLIKANFPPYGRDFLAHNA 67

Query: 69  TGRFCNGKLATDFTA----------------------LTGANFASAASGFYDGTAQLYHA 128
           TGRF NGKLATDFTA                      LTGANFAS ASG+ DGTA  Y+A
Sbjct: 68  TGRFSNGKLATDFTAESLGFTSYPVPYLSQEANGTNLLTGANFASGASGYDDGTAIFYNA 127

Query: 129 VSLTQQLNYYKEYQSKVVNMVGTEKANAIFSGAIHLLSAGSSDFIQ----NTMLILFFTE 188
           ++L QQL  YKEYQ+KV N+VG+E+AN IFSGAIHLLS GSSDF+Q    N +L   FT 
Sbjct: 128 ITLNQQLKNYKEYQNKVTNIVGSERANKIFSGAIHLLSTGSSDFLQSYYINPILNRIFTP 187

Query: 189 HTHHSSSLTFSLHLLKLHSGF-PNLYGMGARRIGVTGLPPLGCLPAAITLFG--SGSNQC 248
                    +S  L+K +S F  NLY +GAR+IGVT LPPLGCLPAAITLFG    +N C
Sbjct: 188 D-------QYSDRLMKPYSTFVQNLYDLGARKIGVTTLPPLGCLPAAITLFGETGNNNTC 247

Query: 249 IQRLNQDAIAFNTKLQSATKSLQNRFSDLKLVAFDIYQPLLNMVSKPAENGFFESRRACC 308
           ++RLNQDA++FNTKL + + +L N    LKLV FDIY PLLNM   P ENGFFESRRACC
Sbjct: 248 VERLNQDAVSFNTKLNNTSMNLTNNLPGLKLVVFDIYNPLLNMAMNPVENGFFESRRACC 307

Query: 309 GTGTVETSFLCNSRSVGTCSNATGYVFWDGFHPTEAANQVLAGDLLTQGFSLI 312
           GTGTVETSFLCN+RSVGTCSNAT YVFWDGFHP+EAAN+V+A +LL QG  LI
Sbjct: 308 GTGTVETSFLCNARSVGTCSNATNYVFWDGFHPSEAANRVIANNLLVQGIPLI 353

BLAST of PI0006323 vs. ExPASy Swiss-Prot
Match: Q0WPI9 (GDSL esterase/lipase At3g53100 OS=Arabidopsis thaliana OX=3702 GN=At3g53100 PE=2 SV=1)

HSP 1 Score: 325.5 bits (833), Expect = 6.7e-88
Identity = 182/357 (50.98%), Postives = 231/357 (64.71%), Query Frame = 0

Query: 2   QLRVIGFLLPFTLLALIFPVAISDPLVPALCIFGDSV--------------------SID 61
           ++RV GF +   LL   F       +VPAL +FGDS+                      D
Sbjct: 3   KMRVSGFRV--LLLVSCFFCKSKGAVVPALIMFGDSIVDVGNNNNLLSIVKSNFLPYGRD 62

Query: 62  FVTHAPTGRFCNGKLATDFTA----------------------LTGANFASAASGFYDGT 121
           F+   PTGRFCNGKLA DF+A                      L GANFASA+SG+YD T
Sbjct: 63  FIDQRPTGRFCNGKLAVDFSAEYLGFSSYPPAFLSREASNENILIGANFASASSGYYDAT 122

Query: 122 AQLYHAVSLTQQLNYYKEYQSKVVNMVGTEKANAIFSGAIHLLSAGSSDFIQ----NTML 181
           +  + ++SLT+QL+YY+ YQ++V  M+G   A  +FS  IH+LSAGSSDF+Q    N +L
Sbjct: 123 SVPFGSISLTRQLSYYRAYQNRVTRMIGRGNARILFSRGIHILSAGSSDFLQNYYINPLL 182

Query: 182 ILFFTEHTHHSSSLTFSLHLLKLHSGF-PNLYGMGARRIGVTGLPPLGCLPAAITLFGSG 241
            +  T          F+  LL+  S F  NLY +GARRIGV  LPP+GCLPAAITLFG+G
Sbjct: 183 NILNTPD-------QFADILLRSFSEFIQNLYELGARRIGVISLPPMGCLPAAITLFGAG 242

Query: 242 SNQCIQRLNQDAIAFNTKLQSATKSLQNRFSDLKLVAFDIYQPLLNMVSKPAENGFFESR 301
           +  C++RLN DAI FNTKL++ T+ L NR S L+LVAF++YQP L++++ P +NGFFE++
Sbjct: 243 NKSCVERLNNDAIMFNTKLENTTRLLMNRHSGLRLVAFNVYQPFLDIITNPTDNGFFETK 302

Query: 302 RACCGTGTVETSFLCNSRSVGTCSNATGYVFWDGFHPTEAANQVLAGDLLTQGFSLI 312
           RACCGTGT+ETSFLCNS S GTC NATGYVFWDGFHPTEA N++LAG LL QG SLI
Sbjct: 303 RACCGTGTIETSFLCNSLSFGTCVNATGYVFWDGFHPTEAVNELLAGQLLGQGISLI 350

BLAST of PI0006323 vs. ExPASy Swiss-Prot
Match: Q9FFC6 (GDSL esterase/lipase At5g22810 OS=Arabidopsis thaliana OX=3702 GN=At5g22810 PE=3 SV=3)

HSP 1 Score: 310.1 bits (793), Expect = 2.9e-83
Identity = 178/354 (50.28%), Postives = 222/354 (62.71%), Query Frame = 0

Query: 5   VIGFLLPFTLLALIFPVAISDPLVPALCIFGDSV--------------------SIDFVT 64
           V G L+ F  + ++  V  + PLVPA+ IFGDSV                      DF T
Sbjct: 13  VFGSLMVFERM-VVMVVMKAQPLVPAIFIFGDSVVDVGNNNDIYTIVKANFPPYGRDFTT 72

Query: 65  HAPTGRFCNGKLATDFTA----------------------LTGANFASAASGFYDGTAQL 124
           H PTGRFCNGKLATDFTA                      L GANFASAASG+YDGTA+L
Sbjct: 73  HTPTGRFCNGKLATDFTAENLGFKSYPQAYLSKKAKGKNLLIGANFASAASGYYDGTAKL 132

Query: 125 YHAVSLTQQLNYYKEYQSKVVNMV---GTEKANAIFSGAIHLLSAGSSDFIQNTML--IL 184
           Y A+SL QQL +YK+Y S++  +        A+AI S  I+++SAGSSDFIQN  +  +L
Sbjct: 133 YSAISLPQQLEHYKDYISRIQEIATSNNNSNASAIISNGIYIVSAGSSDFIQNYYINPLL 192

Query: 185 FFTEHTHHSSSLTFSLHLLKLHSGFPNLYGMGARRIGVTGLPPLGCLPAAITLFGSGSNQ 244
           +  +     S L     +L   S   NLY +GARRIGVT LPPLGCLPAAIT+ G     
Sbjct: 193 YRDQSPDEFSDLL----ILSYSSFIQNLYSLGARRIGVTTLPPLGCLPAAITVVGPHEGG 252

Query: 245 CIQRLNQDAIAFNTKLQSATKSLQNRFSDLKLVAFDIYQPLLNMVSKPAENGFFESRRAC 304
           C ++LN DAI+FN KL + ++ L+     L LV FDIYQPL ++ ++P+E GF E+RRAC
Sbjct: 253 CSEKLNNDAISFNNKLNTTSQDLKRNLIGLNLVVFDIYQPLYDLATRPSEFGFAEARRAC 312

Query: 305 CGTGTVETSFLCNSRSVGTCSNATGYVFWDGFHPTEAANQVLAGDLLTQGFSLI 312
           CGTG +ETS LCN +SVGTC+NAT YVFWDGFHPTEAAN++LA +LL  G SLI
Sbjct: 313 CGTGLLETSILCNPKSVGTCNNATEYVFWDGFHPTEAANKILADNLLVSGISLI 361

BLAST of PI0006323 vs. ExPASy Swiss-Prot
Match: Q9LU14 (GDSL esterase/lipase APG OS=Arabidopsis thaliana OX=3702 GN=APG PE=2 SV=1)

HSP 1 Score: 286.6 bits (732), Expect = 3.4e-76
Identity = 166/356 (46.63%), Postives = 212/356 (59.55%), Query Frame = 0

Query: 12  FTLLALIFPVAISD----PLVPALCIFGDSV--------------------SIDFVTHAP 71
           F LL L+  ++I       LVPA+  FGDSV                      DF  H  
Sbjct: 8   FLLLTLVSTLSILQISFAQLVPAIMTFGDSVVDVGNNNYLPTLFRADYPPYGRDFANHKA 67

Query: 72  TGRFCNGKLATDFTA----------------------LTGANFASAASGFYDGTAQLYHA 131
           TGRFCNGKLATD TA                      L GANFASAASG+ D  A L HA
Sbjct: 68  TGRFCNGKLATDITAETLGFTKYPPAYLSPEASGKNLLIGANFASAASGYDDKAALLNHA 127

Query: 132 VSLTQQLNYYKEYQSKVVNMVGTEKANAIFSGAIHLLSAGSSDFIQNTMLILFFTEHTHH 191
           + L QQ+ Y+KEY+SK++ + G++KA++I  GAI LLSAGSSDF+QN           ++
Sbjct: 128 IPLYQQVEYFKEYKSKLIKIAGSKKADSIIKGAICLLSAGSSDFVQN-----------YY 187

Query: 192 SSSLTFSLHLLKLHSGF---------PNLYGMGARRIGVTGLPPLGCLPAAITLFGSGSN 251
            + L + ++ +  +  F           +Y +GAR+IGVT LPP GCLPAA TLFG    
Sbjct: 188 VNPLLYKVYTVDAYGSFLIDNFSTFIKQVYAVGARKIGVTSLPPTGCLPAARTLFGFHEK 247

Query: 252 QCIQRLNQDAIAFNTKLQSATKSLQNRFSDLKLVAFDIYQPLLNMVSKPAENGFFESRRA 311
            C+ RLN DA  FN KL +A   LQ ++SDLK+V FDIY PL ++V  P+++GF E+ + 
Sbjct: 248 GCVSRLNTDAQNFNKKLNAAASKLQKQYSDLKIVVFDIYSPLYDLVQNPSKSGFTEATKG 307

BLAST of PI0006323 vs. ExPASy TrEMBL
Match: A0A5A7SKR2 (GDSL esterase/lipase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold541G001620 PE=3 SV=1)

HSP 1 Score: 508.1 bits (1307), Expect = 2.7e-140
Identity = 282/356 (79.21%), Postives = 287/356 (80.62%), Query Frame = 0

Query: 1   MQLRVIGFLLPFTLLALIFPVAISDPLVPALCIFGDSV--------------------SI 60
           MQLRVIGFLLPF LLALIFPVAI+DPLVPALCIFGDSV                      
Sbjct: 1   MQLRVIGFLLPFILLALIFPVAITDPLVPALCIFGDSVVDVGNNNNLLTVVKANFPPYGR 60

Query: 61  DFVTHAPTGRFCNGKLATDFTA----------------------LTGANFASAASGFYDG 120
           DFVTH PTGRFCNGKLATDFTA                      LTGANFASAASGFYDG
Sbjct: 61  DFVTHTPTGRFCNGKLATDFTAENLGFSSYPPAYLSQDATGNKLLTGANFASAASGFYDG 120

Query: 121 TAQLYHAVSLTQQLNYYKEYQSKVVNMVGTEKANAIFSGAIHLLSAGSSDFIQNTML--I 180
           TAQLYHAVSLTQQLNYYKEYQSKVVNMVGTEKANAIFSGAIHLLSAGSSDFIQN  +  +
Sbjct: 121 TAQLYHAVSLTQQLNYYKEYQSKVVNMVGTEKANAIFSGAIHLLSAGSSDFIQNYYVNPL 180

Query: 181 LFFTEHTHHSSSLTFSLHLLKLHSGF-PNLYGMGARRIGVTGLPPLGCLPAAITLFGSGS 240
           L+ T      S   FS  L+   S F  NLYGMGARRIGVTGLPPLGCLPAAITLFGSGS
Sbjct: 181 LYRT-----YSPQQFSDILITSFSNFIQNLYGMGARRIGVTGLPPLGCLPAAITLFGSGS 240

Query: 241 NQCIQRLNQDAIAFNTKLQSATKSLQNRFSDLKLVAFDIYQPLLNMVSKPAENGFFESRR 300
           NQCIQRLNQDAIAFNTKLQSAT SLQNRFSDLKLVAFDIYQPLLNMVSKPAENGFFESRR
Sbjct: 241 NQCIQRLNQDAIAFNTKLQSATSSLQNRFSDLKLVAFDIYQPLLNMVSKPAENGFFESRR 300

Query: 301 ACCGTGTVETSFLCNSRSVGTCSNATGYVFWDGFHPTEAANQVLAGDLLTQGFSLI 312
           ACCGTGTVETSFLCNSRSVGTCSNATGYVFWDGFHPTEAANQVLAGDLLTQGFSLI
Sbjct: 301 ACCGTGTVETSFLCNSRSVGTCSNATGYVFWDGFHPTEAANQVLAGDLLTQGFSLI 351

BLAST of PI0006323 vs. ExPASy TrEMBL
Match: A0A1S4E3S2 (GDSL esterase/lipase At5g03820 OS=Cucumis melo OX=3656 GN=LOC103500856 PE=3 SV=1)

HSP 1 Score: 508.1 bits (1307), Expect = 2.7e-140
Identity = 282/356 (79.21%), Postives = 287/356 (80.62%), Query Frame = 0

Query: 1   MQLRVIGFLLPFTLLALIFPVAISDPLVPALCIFGDSV--------------------SI 60
           MQLRVIGFLLPF LLALIFPVAI+DPLVPALCIFGDSV                      
Sbjct: 1   MQLRVIGFLLPFILLALIFPVAITDPLVPALCIFGDSVVDVGNNNNLLTVVKANFPPYGR 60

Query: 61  DFVTHAPTGRFCNGKLATDFTA----------------------LTGANFASAASGFYDG 120
           DFVTH PTGRFCNGKLATDFTA                      LTGANFASAASGFYDG
Sbjct: 61  DFVTHTPTGRFCNGKLATDFTAENLGFSSYPPAYLSQDATGNKLLTGANFASAASGFYDG 120

Query: 121 TAQLYHAVSLTQQLNYYKEYQSKVVNMVGTEKANAIFSGAIHLLSAGSSDFIQNTML--I 180
           TAQLYHAVSLTQQLNYYKEYQSKVVNMVGTEKANAIFSGAIHLLSAGSSDFIQN  +  +
Sbjct: 121 TAQLYHAVSLTQQLNYYKEYQSKVVNMVGTEKANAIFSGAIHLLSAGSSDFIQNYYVNPL 180

Query: 181 LFFTEHTHHSSSLTFSLHLLKLHSGF-PNLYGMGARRIGVTGLPPLGCLPAAITLFGSGS 240
           L+ T      S   FS  L+   S F  NLYGMGARRIGVTGLPPLGCLPAAITLFGSGS
Sbjct: 181 LYRT-----YSPQQFSDILITSFSNFIQNLYGMGARRIGVTGLPPLGCLPAAITLFGSGS 240

Query: 241 NQCIQRLNQDAIAFNTKLQSATKSLQNRFSDLKLVAFDIYQPLLNMVSKPAENGFFESRR 300
           NQCIQRLNQDAIAFNTKLQSAT SLQNRFSDLKLVAFDIYQPLLNMVSKPAENGFFESRR
Sbjct: 241 NQCIQRLNQDAIAFNTKLQSATSSLQNRFSDLKLVAFDIYQPLLNMVSKPAENGFFESRR 300

Query: 301 ACCGTGTVETSFLCNSRSVGTCSNATGYVFWDGFHPTEAANQVLAGDLLTQGFSLI 312
           ACCGTGTVETSFLCNSRSVGTCSNATGYVFWDGFHPTEAANQVLAGDLLTQGFSLI
Sbjct: 301 ACCGTGTVETSFLCNSRSVGTCSNATGYVFWDGFHPTEAANQVLAGDLLTQGFSLI 351

BLAST of PI0006323 vs. ExPASy TrEMBL
Match: A0A5D3C7V6 (GDSL esterase/lipase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold202G00810 PE=3 SV=1)

HSP 1 Score: 506.5 bits (1303), Expect = 7.8e-140
Identity = 281/356 (78.93%), Postives = 286/356 (80.34%), Query Frame = 0

Query: 1   MQLRVIGFLLPFTLLALIFPVAISDPLVPALCIFGDSV--------------------SI 60
           MQLRV GFLLPF LLALIFPVAI+DPLVPALCIFGDSV                      
Sbjct: 1   MQLRVFGFLLPFILLALIFPVAITDPLVPALCIFGDSVVDVGNNNNLLTVVKANFPPYGR 60

Query: 61  DFVTHAPTGRFCNGKLATDFTA----------------------LTGANFASAASGFYDG 120
           DFVTH PTGRFCNGKLATDFTA                      LTGANFASAASGFYDG
Sbjct: 61  DFVTHTPTGRFCNGKLATDFTAENLGFSSYPPAYLSQDATGNKLLTGANFASAASGFYDG 120

Query: 121 TAQLYHAVSLTQQLNYYKEYQSKVVNMVGTEKANAIFSGAIHLLSAGSSDFIQNTML--I 180
           TAQLYHAVSLTQQLNYYKEYQSKVVNMVGTEKANAIFSGAIHLLSAGSSDFIQN  +  +
Sbjct: 121 TAQLYHAVSLTQQLNYYKEYQSKVVNMVGTEKANAIFSGAIHLLSAGSSDFIQNYYVNPL 180

Query: 181 LFFTEHTHHSSSLTFSLHLLKLHSGF-PNLYGMGARRIGVTGLPPLGCLPAAITLFGSGS 240
           L+ T      S   FS  L+   S F  NLYGMGARRIGVTGLPPLGCLPAAITLFGSGS
Sbjct: 181 LYRT-----YSPQQFSDILITSFSNFIQNLYGMGARRIGVTGLPPLGCLPAAITLFGSGS 240

Query: 241 NQCIQRLNQDAIAFNTKLQSATKSLQNRFSDLKLVAFDIYQPLLNMVSKPAENGFFESRR 300
           NQCIQRLNQDAIAFNTKLQSAT SLQNRFSDLKLVAFDIYQPLLNMVSKPAENGFFESRR
Sbjct: 241 NQCIQRLNQDAIAFNTKLQSATSSLQNRFSDLKLVAFDIYQPLLNMVSKPAENGFFESRR 300

Query: 301 ACCGTGTVETSFLCNSRSVGTCSNATGYVFWDGFHPTEAANQVLAGDLLTQGFSLI 312
           ACCGTGTVETSFLCNSRSVGTCSNATGYVFWDGFHPTEAANQVLAGDLLTQGFSLI
Sbjct: 301 ACCGTGTVETSFLCNSRSVGTCSNATGYVFWDGFHPTEAANQVLAGDLLTQGFSLI 351

BLAST of PI0006323 vs. ExPASy TrEMBL
Match: A0A0A0KF82 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G449250 PE=3 SV=1)

HSP 1 Score: 492.7 bits (1267), Expect = 1.2e-135
Identity = 276/356 (77.53%), Postives = 282/356 (79.21%), Query Frame = 0

Query: 1   MQLRVIGFLLPFTLLALIFPVAISDPLVPALCIFGDSV--------------------SI 60
           MQLRVIGFLLPF LL LIFPVAI+DPLVPALCIFGDSV                      
Sbjct: 1   MQLRVIGFLLPFILLPLIFPVAITDPLVPALCIFGDSVVDVGNNNNLLTVVKANFPPYGR 60

Query: 61  DFVTHAPTGRFCNGKLATDFTA----------------------LTGANFASAASGFYDG 120
           DFVTHAPTGRFCNGKLATD TA                      LTGANFASAASGFYDG
Sbjct: 61  DFVTHAPTGRFCNGKLATDITAELLGFSSYPPAYLSQDATGNKLLTGANFASAASGFYDG 120

Query: 121 TAQLYHAVSLTQQLNYYKEYQSKVVNMVGTEKANAIFSGAIHLLSAGSSDFIQNTML--I 180
           TAQLYHAVSLTQQLNYYKEYQSKVVNMVGTEKANAIFSGAIHLLSAGSSDFIQN  +  +
Sbjct: 121 TAQLYHAVSLTQQLNYYKEYQSKVVNMVGTEKANAIFSGAIHLLSAGSSDFIQNYYVNPL 180

Query: 181 LFFTEHTHHSSSLTFSLHLLKLHSGF-PNLYGMGARRIGVTGLPPLGCLPAAITLFGSGS 240
           L+ T      S   FS  L+   S F  NLYGMGARRIGVTGLPPLGCLPAAITLFGSGS
Sbjct: 181 LYRT-----YSPQQFSDILITSFSNFAQNLYGMGARRIGVTGLPPLGCLPAAITLFGSGS 240

Query: 241 NQCIQRLNQDAIAFNTKLQSATKSLQNRFSDLKLVAFDIYQPLLNMVSKPAENGFFESRR 300
           NQCIQRLNQDAIAFNTKLQSAT SLQ RFSDLKLVAFDIYQPLLNMVSKPAENGFFESRR
Sbjct: 241 NQCIQRLNQDAIAFNTKLQSATTSLQKRFSDLKLVAFDIYQPLLNMVSKPAENGFFESRR 300

Query: 301 ACCGTGTVETSFLCNSRSVGTCSNATGYVFWDGFHPTEAANQVLAGDLLTQGFSLI 312
           ACCGTGTVETSFLCN+ SVGTCSNATGYVFWDGFHPTEAANQVLA  LLTQGFSLI
Sbjct: 301 ACCGTGTVETSFLCNNISVGTCSNATGYVFWDGFHPTEAANQVLAEGLLTQGFSLI 351

BLAST of PI0006323 vs. ExPASy TrEMBL
Match: A0A6J1I5T7 (GDSL esterase/lipase At5g03820-like OS=Cucurbita maxima OX=3661 GN=LOC111471301 PE=3 SV=1)

HSP 1 Score: 470.7 bits (1210), Expect = 4.7e-129
Identity = 256/356 (71.91%), Postives = 278/356 (78.09%), Query Frame = 0

Query: 1   MQLRVIGFLLPFTLLALIFPVAISDPLVPALCIFGDSV--------------------SI 60
           MQL   GFL+ F+LLAL+FPVAISDPLVPALCIFGDSV                      
Sbjct: 1   MQLPAAGFLVTFSLLALVFPVAISDPLVPALCIFGDSVVDVGNNNNLLTVVKANFPPYGR 60

Query: 61  DFVTHAPTGRFCNGKLATDFTA----------------------LTGANFASAASGFYDG 120
           DFVTHAPTGRFCNGKLATDFTA                      LTGANFASAASGFYDG
Sbjct: 61  DFVTHAPTGRFCNGKLATDFTAELLGFSSYPPAYLGQDATGNKLLTGANFASAASGFYDG 120

Query: 121 TAQLYHAVSLTQQLNYYKEYQSKVVNMVGTEKANAIFSGAIHLLSAGSSDFIQNTML--I 180
           TAQLYH++SLTQQLNYYKEYQSKVVNMVGT K NAIFSG IHLLSAGSSDFIQN  +  +
Sbjct: 121 TAQLYHSISLTQQLNYYKEYQSKVVNMVGTGKGNAIFSGGIHLLSAGSSDFIQNYYVNPL 180

Query: 181 LFFTEHTHHSSSLTFSLHLLKLHSGF-PNLYGMGARRIGVTGLPPLGCLPAAITLFGSGS 240
           L+ T      S   FS  L+  +S F  NLYG+GARRIGVTGLPP+GCLPAAITLFGSGS
Sbjct: 181 LYRT-----YSPQQFSDILITSYSNFIQNLYGLGARRIGVTGLPPMGCLPAAITLFGSGS 240

Query: 241 NQCIQRLNQDAIAFNTKLQSATKSLQNRFSDLKLVAFDIYQPLLNMVSKPAENGFFESRR 300
           NQCIQRLNQDA+AFNTKLQS ++SLQNRFSDLKLV FDIYQPLLNMVSKPA+NGFFE+RR
Sbjct: 241 NQCIQRLNQDAMAFNTKLQSTSQSLQNRFSDLKLVVFDIYQPLLNMVSKPADNGFFEARR 300

Query: 301 ACCGTGTVETSFLCNSRSVGTCSNATGYVFWDGFHPTEAANQVLAGDLLTQGFSLI 312
           ACCGTGTVETS LCN+ S+GTCSN TGYVFWDGFHPTEAAN+VLA DL++QGFSLI
Sbjct: 301 ACCGTGTVETSLLCNNGSIGTCSNVTGYVFWDGFHPTEAANKVLAADLISQGFSLI 351

BLAST of PI0006323 vs. NCBI nr
Match: XP_016902881.1 (PREDICTED: GDSL esterase/lipase At5g03820 [Cucumis melo] >KAA0025307.1 GDSL esterase/lipase [Cucumis melo var. makuwa])

HSP 1 Score: 508.1 bits (1307), Expect = 5.5e-140
Identity = 282/356 (79.21%), Postives = 287/356 (80.62%), Query Frame = 0

Query: 1   MQLRVIGFLLPFTLLALIFPVAISDPLVPALCIFGDSV--------------------SI 60
           MQLRVIGFLLPF LLALIFPVAI+DPLVPALCIFGDSV                      
Sbjct: 1   MQLRVIGFLLPFILLALIFPVAITDPLVPALCIFGDSVVDVGNNNNLLTVVKANFPPYGR 60

Query: 61  DFVTHAPTGRFCNGKLATDFTA----------------------LTGANFASAASGFYDG 120
           DFVTH PTGRFCNGKLATDFTA                      LTGANFASAASGFYDG
Sbjct: 61  DFVTHTPTGRFCNGKLATDFTAENLGFSSYPPAYLSQDATGNKLLTGANFASAASGFYDG 120

Query: 121 TAQLYHAVSLTQQLNYYKEYQSKVVNMVGTEKANAIFSGAIHLLSAGSSDFIQNTML--I 180
           TAQLYHAVSLTQQLNYYKEYQSKVVNMVGTEKANAIFSGAIHLLSAGSSDFIQN  +  +
Sbjct: 121 TAQLYHAVSLTQQLNYYKEYQSKVVNMVGTEKANAIFSGAIHLLSAGSSDFIQNYYVNPL 180

Query: 181 LFFTEHTHHSSSLTFSLHLLKLHSGF-PNLYGMGARRIGVTGLPPLGCLPAAITLFGSGS 240
           L+ T      S   FS  L+   S F  NLYGMGARRIGVTGLPPLGCLPAAITLFGSGS
Sbjct: 181 LYRT-----YSPQQFSDILITSFSNFIQNLYGMGARRIGVTGLPPLGCLPAAITLFGSGS 240

Query: 241 NQCIQRLNQDAIAFNTKLQSATKSLQNRFSDLKLVAFDIYQPLLNMVSKPAENGFFESRR 300
           NQCIQRLNQDAIAFNTKLQSAT SLQNRFSDLKLVAFDIYQPLLNMVSKPAENGFFESRR
Sbjct: 241 NQCIQRLNQDAIAFNTKLQSATSSLQNRFSDLKLVAFDIYQPLLNMVSKPAENGFFESRR 300

Query: 301 ACCGTGTVETSFLCNSRSVGTCSNATGYVFWDGFHPTEAANQVLAGDLLTQGFSLI 312
           ACCGTGTVETSFLCNSRSVGTCSNATGYVFWDGFHPTEAANQVLAGDLLTQGFSLI
Sbjct: 301 ACCGTGTVETSFLCNSRSVGTCSNATGYVFWDGFHPTEAANQVLAGDLLTQGFSLI 351

BLAST of PI0006323 vs. NCBI nr
Match: TYK07362.1 (GDSL esterase/lipase [Cucumis melo var. makuwa])

HSP 1 Score: 506.5 bits (1303), Expect = 1.6e-139
Identity = 281/356 (78.93%), Postives = 286/356 (80.34%), Query Frame = 0

Query: 1   MQLRVIGFLLPFTLLALIFPVAISDPLVPALCIFGDSV--------------------SI 60
           MQLRV GFLLPF LLALIFPVAI+DPLVPALCIFGDSV                      
Sbjct: 1   MQLRVFGFLLPFILLALIFPVAITDPLVPALCIFGDSVVDVGNNNNLLTVVKANFPPYGR 60

Query: 61  DFVTHAPTGRFCNGKLATDFTA----------------------LTGANFASAASGFYDG 120
           DFVTH PTGRFCNGKLATDFTA                      LTGANFASAASGFYDG
Sbjct: 61  DFVTHTPTGRFCNGKLATDFTAENLGFSSYPPAYLSQDATGNKLLTGANFASAASGFYDG 120

Query: 121 TAQLYHAVSLTQQLNYYKEYQSKVVNMVGTEKANAIFSGAIHLLSAGSSDFIQNTML--I 180
           TAQLYHAVSLTQQLNYYKEYQSKVVNMVGTEKANAIFSGAIHLLSAGSSDFIQN  +  +
Sbjct: 121 TAQLYHAVSLTQQLNYYKEYQSKVVNMVGTEKANAIFSGAIHLLSAGSSDFIQNYYVNPL 180

Query: 181 LFFTEHTHHSSSLTFSLHLLKLHSGF-PNLYGMGARRIGVTGLPPLGCLPAAITLFGSGS 240
           L+ T      S   FS  L+   S F  NLYGMGARRIGVTGLPPLGCLPAAITLFGSGS
Sbjct: 181 LYRT-----YSPQQFSDILITSFSNFIQNLYGMGARRIGVTGLPPLGCLPAAITLFGSGS 240

Query: 241 NQCIQRLNQDAIAFNTKLQSATKSLQNRFSDLKLVAFDIYQPLLNMVSKPAENGFFESRR 300
           NQCIQRLNQDAIAFNTKLQSAT SLQNRFSDLKLVAFDIYQPLLNMVSKPAENGFFESRR
Sbjct: 241 NQCIQRLNQDAIAFNTKLQSATSSLQNRFSDLKLVAFDIYQPLLNMVSKPAENGFFESRR 300

Query: 301 ACCGTGTVETSFLCNSRSVGTCSNATGYVFWDGFHPTEAANQVLAGDLLTQGFSLI 312
           ACCGTGTVETSFLCNSRSVGTCSNATGYVFWDGFHPTEAANQVLAGDLLTQGFSLI
Sbjct: 301 ACCGTGTVETSFLCNSRSVGTCSNATGYVFWDGFHPTEAANQVLAGDLLTQGFSLI 351

BLAST of PI0006323 vs. NCBI nr
Match: XP_038881618.1 (GDSL esterase/lipase At5g03820 [Benincasa hispida])

HSP 1 Score: 493.4 bits (1269), Expect = 1.4e-135
Identity = 269/355 (75.77%), Postives = 280/355 (78.87%), Query Frame = 0

Query: 1   MQLRVIGFLLPFTLLALIFPVAISDPLVPALCIFGDSV--------------------SI 60
           MQL V GFLLPF+LLALIFPVAIS PLVPALCIFGDSV                      
Sbjct: 1   MQLPVSGFLLPFSLLALIFPVAISGPLVPALCIFGDSVVDVGNNNNLLTVVKANFPPYGR 60

Query: 61  DFVTHAPTGRFCNGKLATDFTA----------------------LTGANFASAASGFYDG 120
           DFVTH PTGRFCNGKLATDFTA                      LTGANFASAASGFYDG
Sbjct: 61  DFVTHTPTGRFCNGKLATDFTAEFLGFSSYPPAYLSQDATGNKLLTGANFASAASGFYDG 120

Query: 121 TAQLYHAVSLTQQLNYYKEYQSKVVNMVGTEKANAIFSGAIHLLSAGSSDFIQNTML--I 180
           TAQLYHAVSLTQQLNYYKEYQSKVVNMVGTEK N IFSGAIHLLSAGSSDFIQN  +  +
Sbjct: 121 TAQLYHAVSLTQQLNYYKEYQSKVVNMVGTEKGNTIFSGAIHLLSAGSSDFIQNYYVNPL 180

Query: 181 LFFTEHTHHSSSLTFSLHLLKLHSGFPNLYGMGARRIGVTGLPPLGCLPAAITLFGSGSN 240
           L+ T      S +     +    S   NLYG+GARRIGVTGLPPLGCLPAAITLFGSGSN
Sbjct: 181 LYRTYSPQQFSDIL----ITSFSSFIQNLYGLGARRIGVTGLPPLGCLPAAITLFGSGSN 240

Query: 241 QCIQRLNQDAIAFNTKLQSATKSLQNRFSDLKLVAFDIYQPLLNMVSKPAENGFFESRRA 300
           QCIQRLNQDAIAFNTKLQS ++SLQNRFSDLKLVAFDIYQPLL+MVSKPAENGFFESRRA
Sbjct: 241 QCIQRLNQDAIAFNTKLQSTSQSLQNRFSDLKLVAFDIYQPLLDMVSKPAENGFFESRRA 300

Query: 301 CCGTGTVETSFLCNSRSVGTCSNATGYVFWDGFHPTEAANQVLAGDLLTQGFSLI 312
           CCGTGTVETSFLCNSRS+GTCSNATGYVFWDGFHPTEAANQVLAGDLLTQGFSLI
Sbjct: 301 CCGTGTVETSFLCNSRSIGTCSNATGYVFWDGFHPTEAANQVLAGDLLTQGFSLI 351

BLAST of PI0006323 vs. NCBI nr
Match: XP_004143292.1 (GDSL esterase/lipase At5g03810 [Cucumis sativus] >KGN48218.1 hypothetical protein Csa_003335 [Cucumis sativus])

HSP 1 Score: 492.7 bits (1267), Expect = 2.4e-135
Identity = 276/356 (77.53%), Postives = 282/356 (79.21%), Query Frame = 0

Query: 1   MQLRVIGFLLPFTLLALIFPVAISDPLVPALCIFGDSV--------------------SI 60
           MQLRVIGFLLPF LL LIFPVAI+DPLVPALCIFGDSV                      
Sbjct: 1   MQLRVIGFLLPFILLPLIFPVAITDPLVPALCIFGDSVVDVGNNNNLLTVVKANFPPYGR 60

Query: 61  DFVTHAPTGRFCNGKLATDFTA----------------------LTGANFASAASGFYDG 120
           DFVTHAPTGRFCNGKLATD TA                      LTGANFASAASGFYDG
Sbjct: 61  DFVTHAPTGRFCNGKLATDITAELLGFSSYPPAYLSQDATGNKLLTGANFASAASGFYDG 120

Query: 121 TAQLYHAVSLTQQLNYYKEYQSKVVNMVGTEKANAIFSGAIHLLSAGSSDFIQNTML--I 180
           TAQLYHAVSLTQQLNYYKEYQSKVVNMVGTEKANAIFSGAIHLLSAGSSDFIQN  +  +
Sbjct: 121 TAQLYHAVSLTQQLNYYKEYQSKVVNMVGTEKANAIFSGAIHLLSAGSSDFIQNYYVNPL 180

Query: 181 LFFTEHTHHSSSLTFSLHLLKLHSGF-PNLYGMGARRIGVTGLPPLGCLPAAITLFGSGS 240
           L+ T      S   FS  L+   S F  NLYGMGARRIGVTGLPPLGCLPAAITLFGSGS
Sbjct: 181 LYRT-----YSPQQFSDILITSFSNFAQNLYGMGARRIGVTGLPPLGCLPAAITLFGSGS 240

Query: 241 NQCIQRLNQDAIAFNTKLQSATKSLQNRFSDLKLVAFDIYQPLLNMVSKPAENGFFESRR 300
           NQCIQRLNQDAIAFNTKLQSAT SLQ RFSDLKLVAFDIYQPLLNMVSKPAENGFFESRR
Sbjct: 241 NQCIQRLNQDAIAFNTKLQSATTSLQKRFSDLKLVAFDIYQPLLNMVSKPAENGFFESRR 300

Query: 301 ACCGTGTVETSFLCNSRSVGTCSNATGYVFWDGFHPTEAANQVLAGDLLTQGFSLI 312
           ACCGTGTVETSFLCN+ SVGTCSNATGYVFWDGFHPTEAANQVLA  LLTQGFSLI
Sbjct: 301 ACCGTGTVETSFLCNNISVGTCSNATGYVFWDGFHPTEAANQVLAEGLLTQGFSLI 351

BLAST of PI0006323 vs. NCBI nr
Match: XP_022972797.1 (GDSL esterase/lipase At5g03820-like [Cucurbita maxima])

HSP 1 Score: 470.7 bits (1210), Expect = 9.8e-129
Identity = 256/356 (71.91%), Postives = 278/356 (78.09%), Query Frame = 0

Query: 1   MQLRVIGFLLPFTLLALIFPVAISDPLVPALCIFGDSV--------------------SI 60
           MQL   GFL+ F+LLAL+FPVAISDPLVPALCIFGDSV                      
Sbjct: 1   MQLPAAGFLVTFSLLALVFPVAISDPLVPALCIFGDSVVDVGNNNNLLTVVKANFPPYGR 60

Query: 61  DFVTHAPTGRFCNGKLATDFTA----------------------LTGANFASAASGFYDG 120
           DFVTHAPTGRFCNGKLATDFTA                      LTGANFASAASGFYDG
Sbjct: 61  DFVTHAPTGRFCNGKLATDFTAELLGFSSYPPAYLGQDATGNKLLTGANFASAASGFYDG 120

Query: 121 TAQLYHAVSLTQQLNYYKEYQSKVVNMVGTEKANAIFSGAIHLLSAGSSDFIQNTML--I 180
           TAQLYH++SLTQQLNYYKEYQSKVVNMVGT K NAIFSG IHLLSAGSSDFIQN  +  +
Sbjct: 121 TAQLYHSISLTQQLNYYKEYQSKVVNMVGTGKGNAIFSGGIHLLSAGSSDFIQNYYVNPL 180

Query: 181 LFFTEHTHHSSSLTFSLHLLKLHSGF-PNLYGMGARRIGVTGLPPLGCLPAAITLFGSGS 240
           L+ T      S   FS  L+  +S F  NLYG+GARRIGVTGLPP+GCLPAAITLFGSGS
Sbjct: 181 LYRT-----YSPQQFSDILITSYSNFIQNLYGLGARRIGVTGLPPMGCLPAAITLFGSGS 240

Query: 241 NQCIQRLNQDAIAFNTKLQSATKSLQNRFSDLKLVAFDIYQPLLNMVSKPAENGFFESRR 300
           NQCIQRLNQDA+AFNTKLQS ++SLQNRFSDLKLV FDIYQPLLNMVSKPA+NGFFE+RR
Sbjct: 241 NQCIQRLNQDAMAFNTKLQSTSQSLQNRFSDLKLVVFDIYQPLLNMVSKPADNGFFEARR 300

Query: 301 ACCGTGTVETSFLCNSRSVGTCSNATGYVFWDGFHPTEAANQVLAGDLLTQGFSLI 312
           ACCGTGTVETS LCN+ S+GTCSN TGYVFWDGFHPTEAAN+VLA DL++QGFSLI
Sbjct: 301 ACCGTGTVETSLLCNNGSIGTCSNVTGYVFWDGFHPTEAANKVLAADLISQGFSLI 351

BLAST of PI0006323 vs. TAIR 10
Match: AT5G03810.1 (GDSL-like Lipase/Acylhydrolase family protein )

HSP 1 Score: 351.7 bits (901), Expect = 6.2e-97
Identity = 199/339 (58.70%), Postives = 228/339 (67.26%), Query Frame = 0

Query: 21  VAISDPLVPALCIFGDSV--------------------SIDFVTHAPTGRFCNGKLATDF 80
           V   + LVPAL I GDSV                      DFV H+ TGRF NGKLATDF
Sbjct: 21  VGTGETLVPALIIMGDSVVDAGNNNHRITLVKANFPPYGRDFVAHSATGRFSNGKLATDF 80

Query: 81  TA----------------------LTGANFASAASGFYDGTAQLYHAVSLTQQLNYYKEY 140
           TA                      LTGANFAS ASGF D TA  Y+A++L+QQL  YKEY
Sbjct: 81  TAENLGFTSYPVAYLSQEANETNLLTGANFASGASGFDDATAIFYNAITLSQQLKNYKEY 140

Query: 141 QSKVVNMVGTEKANAIFSGAIHLLSAGSSDFIQ----NTMLILFFTEHTHHSSSLTFSLH 200
           Q+KV N+VG E+AN IFSGAIHLLS GSSDF+Q    N +L   FT          +S H
Sbjct: 141 QNKVTNIVGKERANEIFSGAIHLLSTGSSDFLQSYYINPILNRIFTPD-------QYSDH 200

Query: 201 LLKLHSGF-PNLYGMGARRIGVTGLPPLGCLPAAITLFGS-GSNQCIQRLNQDAIAFNTK 260
           LL+ +S F  NLYG+GARRIGVT LPPLGCLPAAITLFG  G+N C++RLNQDA++FNTK
Sbjct: 201 LLRSYSTFVQNLYGLGARRIGVTTLPPLGCLPAAITLFGGVGNNMCVERLNQDAVSFNTK 260

Query: 261 LQSATKSLQNRFSDLKLVAFDIYQPLLNMVSKPAENGFFESRRACCGTGTVETSFLCNSR 312
           L + + +L N    LKLV FDIY PLLNMV  P E GFFESRRACCGTGT+ETSFLCN+ 
Sbjct: 261 LNNTSINLTNNLPGLKLVVFDIYNPLLNMVINPVEYGFFESRRACCGTGTMETSFLCNAL 320

BLAST of PI0006323 vs. TAIR 10
Match: AT5G03820.1 (GDSL-like Lipase/Acylhydrolase family protein )

HSP 1 Score: 350.5 bits (898), Expect = 1.4e-96
Identity = 200/353 (56.66%), Postives = 235/353 (66.57%), Query Frame = 0

Query: 9   LLPFTLLALIFP-VAISDPLVPALCIFGDSV--------------------SIDFVTHAP 68
           L+ F+++A  +  V   +PLVPAL I GDSV                      DF+ H  
Sbjct: 8   LMTFSVIACFYAGVGTGEPLVPALIIMGDSVVDAGNNNRLNTLIKANFPPYGRDFLAHNA 67

Query: 69  TGRFCNGKLATDFTA----------------------LTGANFASAASGFYDGTAQLYHA 128
           TGRF NGKLATDFTA                      LTGANFAS ASG+ DGTA  Y+A
Sbjct: 68  TGRFSNGKLATDFTAESLGFTSYPVPYLSQEANGTNLLTGANFASGASGYDDGTAIFYNA 127

Query: 129 VSLTQQLNYYKEYQSKVVNMVGTEKANAIFSGAIHLLSAGSSDFIQ----NTMLILFFTE 188
           ++L QQL  YKEYQ+KV N+VG+E+AN IFSGAIHLLS GSSDF+Q    N +L   FT 
Sbjct: 128 ITLNQQLKNYKEYQNKVTNIVGSERANKIFSGAIHLLSTGSSDFLQSYYINPILNRIFTP 187

Query: 189 HTHHSSSLTFSLHLLKLHSGF-PNLYGMGARRIGVTGLPPLGCLPAAITLFG--SGSNQC 248
                    +S  L+K +S F  NLY +GAR+IGVT LPPLGCLPAAITLFG    +N C
Sbjct: 188 D-------QYSDRLMKPYSTFVQNLYDLGARKIGVTTLPPLGCLPAAITLFGETGNNNTC 247

Query: 249 IQRLNQDAIAFNTKLQSATKSLQNRFSDLKLVAFDIYQPLLNMVSKPAENGFFESRRACC 308
           ++RLNQDA++FNTKL + + +L N    LKLV FDIY PLLNM   P ENGFFESRRACC
Sbjct: 248 VERLNQDAVSFNTKLNNTSMNLTNNLPGLKLVVFDIYNPLLNMAMNPVENGFFESRRACC 307

Query: 309 GTGTVETSFLCNSRSVGTCSNATGYVFWDGFHPTEAANQVLAGDLLTQGFSLI 312
           GTGTVETSFLCN+RSVGTCSNAT YVFWDGFHP+EAAN+V+A +LL QG  LI
Sbjct: 308 GTGTVETSFLCNARSVGTCSNATNYVFWDGFHPSEAANRVIANNLLVQGIPLI 353

BLAST of PI0006323 vs. TAIR 10
Match: AT3G53100.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 325.5 bits (833), Expect = 4.7e-89
Identity = 182/357 (50.98%), Postives = 231/357 (64.71%), Query Frame = 0

Query: 2   QLRVIGFLLPFTLLALIFPVAISDPLVPALCIFGDSV--------------------SID 61
           ++RV GF +   LL   F       +VPAL +FGDS+                      D
Sbjct: 3   KMRVSGFRV--LLLVSCFFCKSKGAVVPALIMFGDSIVDVGNNNNLLSIVKSNFLPYGRD 62

Query: 62  FVTHAPTGRFCNGKLATDFTA----------------------LTGANFASAASGFYDGT 121
           F+   PTGRFCNGKLA DF+A                      L GANFASA+SG+YD T
Sbjct: 63  FIDQRPTGRFCNGKLAVDFSAEYLGFSSYPPAFLSREASNENILIGANFASASSGYYDAT 122

Query: 122 AQLYHAVSLTQQLNYYKEYQSKVVNMVGTEKANAIFSGAIHLLSAGSSDFIQ----NTML 181
           +  + ++SLT+QL+YY+ YQ++V  M+G   A  +FS  IH+LSAGSSDF+Q    N +L
Sbjct: 123 SVPFGSISLTRQLSYYRAYQNRVTRMIGRGNARILFSRGIHILSAGSSDFLQNYYINPLL 182

Query: 182 ILFFTEHTHHSSSLTFSLHLLKLHSGF-PNLYGMGARRIGVTGLPPLGCLPAAITLFGSG 241
            +  T          F+  LL+  S F  NLY +GARRIGV  LPP+GCLPAAITLFG+G
Sbjct: 183 NILNTPD-------QFADILLRSFSEFIQNLYELGARRIGVISLPPMGCLPAAITLFGAG 242

Query: 242 SNQCIQRLNQDAIAFNTKLQSATKSLQNRFSDLKLVAFDIYQPLLNMVSKPAENGFFESR 301
           +  C++RLN DAI FNTKL++ T+ L NR S L+LVAF++YQP L++++ P +NGFFE++
Sbjct: 243 NKSCVERLNNDAIMFNTKLENTTRLLMNRHSGLRLVAFNVYQPFLDIITNPTDNGFFETK 302

Query: 302 RACCGTGTVETSFLCNSRSVGTCSNATGYVFWDGFHPTEAANQVLAGDLLTQGFSLI 312
           RACCGTGT+ETSFLCNS S GTC NATGYVFWDGFHPTEA N++LAG LL QG SLI
Sbjct: 303 RACCGTGTIETSFLCNSLSFGTCVNATGYVFWDGFHPTEAVNELLAGQLLGQGISLI 350

BLAST of PI0006323 vs. TAIR 10
Match: AT5G22810.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 311.6 bits (797), Expect = 7.1e-85
Identity = 173/335 (51.64%), Postives = 213/335 (63.58%), Query Frame = 0

Query: 24  SDPLVPALCIFGDSV--------------------SIDFVTHAPTGRFCNGKLATDFTA- 83
           + PLVPA+ IFGDSV                      DF TH PTGRFCNGKLATDFTA 
Sbjct: 6   AQPLVPAIFIFGDSVVDVGNNNDIYTIVKANFPPYGRDFTTHTPTGRFCNGKLATDFTAE 65

Query: 84  ---------------------LTGANFASAASGFYDGTAQLYHAVSLTQQLNYYKEYQSK 143
                                L GANFASAASG+YDGTA+LY A+SL QQL +YK+Y S+
Sbjct: 66  NLGFKSYPQAYLSKKAKGKNLLIGANFASAASGYYDGTAKLYSAISLPQQLEHYKDYISR 125

Query: 144 VVNMV---GTEKANAIFSGAIHLLSAGSSDFIQNTML--ILFFTEHTHHSSSLTFSLHLL 203
           +  +        A+AI S  I+++SAGSSDFIQN  +  +L+  +     S L     +L
Sbjct: 126 IQEIATSNNNSNASAIISNGIYIVSAGSSDFIQNYYINPLLYRDQSPDEFSDLL----IL 185

Query: 204 KLHSGFPNLYGMGARRIGVTGLPPLGCLPAAITLFGSGSNQCIQRLNQDAIAFNTKLQSA 263
              S   NLY +GARRIGVT LPPLGCLPAAIT+ G     C ++LN DAI+FN KL + 
Sbjct: 186 SYSSFIQNLYSLGARRIGVTTLPPLGCLPAAITVVGPHEGGCSEKLNNDAISFNNKLNTT 245

Query: 264 TKSLQNRFSDLKLVAFDIYQPLLNMVSKPAENGFFESRRACCGTGTVETSFLCNSRSVGT 312
           ++ L+     L LV FDIYQPL ++ ++P+E GF E+RRACCGTG +ETS LCN +SVGT
Sbjct: 246 SQDLKRNLIGLNLVVFDIYQPLYDLATRPSEFGFAEARRACCGTGLLETSILCNPKSVGT 305

BLAST of PI0006323 vs. TAIR 10
Match: AT3G16370.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 286.6 bits (732), Expect = 2.4e-77
Identity = 166/356 (46.63%), Postives = 212/356 (59.55%), Query Frame = 0

Query: 12  FTLLALIFPVAISD----PLVPALCIFGDSV--------------------SIDFVTHAP 71
           F LL L+  ++I       LVPA+  FGDSV                      DF  H  
Sbjct: 8   FLLLTLVSTLSILQISFAQLVPAIMTFGDSVVDVGNNNYLPTLFRADYPPYGRDFANHKA 67

Query: 72  TGRFCNGKLATDFTA----------------------LTGANFASAASGFYDGTAQLYHA 131
           TGRFCNGKLATD TA                      L GANFASAASG+ D  A L HA
Sbjct: 68  TGRFCNGKLATDITAETLGFTKYPPAYLSPEASGKNLLIGANFASAASGYDDKAALLNHA 127

Query: 132 VSLTQQLNYYKEYQSKVVNMVGTEKANAIFSGAIHLLSAGSSDFIQNTMLILFFTEHTHH 191
           + L QQ+ Y+KEY+SK++ + G++KA++I  GAI LLSAGSSDF+QN           ++
Sbjct: 128 IPLYQQVEYFKEYKSKLIKIAGSKKADSIIKGAICLLSAGSSDFVQN-----------YY 187

Query: 192 SSSLTFSLHLLKLHSGF---------PNLYGMGARRIGVTGLPPLGCLPAAITLFGSGSN 251
            + L + ++ +  +  F           +Y +GAR+IGVT LPP GCLPAA TLFG    
Sbjct: 188 VNPLLYKVYTVDAYGSFLIDNFSTFIKQVYAVGARKIGVTSLPPTGCLPAARTLFGFHEK 247

Query: 252 QCIQRLNQDAIAFNTKLQSATKSLQNRFSDLKLVAFDIYQPLLNMVSKPAENGFFESRRA 311
            C+ RLN DA  FN KL +A   LQ ++SDLK+V FDIY PL ++V  P+++GF E+ + 
Sbjct: 248 GCVSRLNTDAQNFNKKLNAAASKLQKQYSDLKIVVFDIYSPLYDLVQNPSKSGFTEATKG 307

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FFN08.7e-9658.70GDSL esterase/lipase At5g03810 OS=Arabidopsis thaliana OX=3702 GN=At5g03810 PE=3... [more]
Q9LZC51.9e-9556.66GDSL esterase/lipase At5g03820 OS=Arabidopsis thaliana OX=3702 GN=At5g03820 PE=3... [more]
Q0WPI96.7e-8850.98GDSL esterase/lipase At3g53100 OS=Arabidopsis thaliana OX=3702 GN=At3g53100 PE=2... [more]
Q9FFC62.9e-8350.28GDSL esterase/lipase At5g22810 OS=Arabidopsis thaliana OX=3702 GN=At5g22810 PE=3... [more]
Q9LU143.4e-7646.63GDSL esterase/lipase APG OS=Arabidopsis thaliana OX=3702 GN=APG PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A5A7SKR22.7e-14079.21GDSL esterase/lipase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold541... [more]
A0A1S4E3S22.7e-14079.21GDSL esterase/lipase At5g03820 OS=Cucumis melo OX=3656 GN=LOC103500856 PE=3 SV=1[more]
A0A5D3C7V67.8e-14078.93GDSL esterase/lipase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold202... [more]
A0A0A0KF821.2e-13577.53Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G449250 PE=3 SV=1[more]
A0A6J1I5T74.7e-12971.91GDSL esterase/lipase At5g03820-like OS=Cucurbita maxima OX=3661 GN=LOC111471301 ... [more]
Match NameE-valueIdentityDescription
XP_016902881.15.5e-14079.21PREDICTED: GDSL esterase/lipase At5g03820 [Cucumis melo] >KAA0025307.1 GDSL este... [more]
TYK07362.11.6e-13978.93GDSL esterase/lipase [Cucumis melo var. makuwa][more]
XP_038881618.11.4e-13575.77GDSL esterase/lipase At5g03820 [Benincasa hispida][more]
XP_004143292.12.4e-13577.53GDSL esterase/lipase At5g03810 [Cucumis sativus] >KGN48218.1 hypothetical protei... [more]
XP_022972797.19.8e-12971.91GDSL esterase/lipase At5g03820-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT5G03810.16.2e-9758.70GDSL-like Lipase/Acylhydrolase family protein [more]
AT5G03820.11.4e-9656.66GDSL-like Lipase/Acylhydrolase family protein [more]
AT3G53100.14.7e-8950.98GDSL-like Lipase/Acylhydrolase superfamily protein [more]
AT5G22810.17.1e-8551.64GDSL-like Lipase/Acylhydrolase superfamily protein [more]
AT3G16370.12.4e-7746.63GDSL-like Lipase/Acylhydrolase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036514SGNH hydrolase superfamilyGENE3D3.40.50.1110SGNH hydrolasecoord: 24..307
e-value: 4.5E-49
score: 169.6
IPR001087GDSL lipase/esterasePFAMPF00657Lipase_GDSLcoord: 33..300
e-value: 4.1E-24
score: 86.3
NoneNo IPR availablePANTHERPTHR45642:SF103GDSL-MOTIF LIPASEcoord: 63..310
NoneNo IPR availablePANTHERPTHR45642:SF103GDSL-MOTIF LIPASEcoord: 10..62
NoneNo IPR availablePANTHERPTHR45642GDSL ESTERASE/LIPASE EXL3coord: 10..62
coord: 63..310
IPR035669GDSL lipase/esterase-like, plantCDDcd01837SGNH_plant_lipase_likecoord: 29..305
e-value: 1.51046E-94
score: 280.656

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0006323.1PI0006323.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016788 hydrolase activity, acting on ester bonds