PI0005478 (gene) Melon (PI 482460) v1

Overview


Name	PI0005478
Type	gene
Organism	Cucumis metuliferus (Melon (PI 482460) v1)
Description	Reverse transcriptase
Location	chr04: 24575981 .. 24576489 (+)
RNA-Seq Expression	PI0005478
Synteny	PI0005478

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCCCGATAGTTTGCCCAAATTTCTACCGCCGAGAAGGATAATTGACCACGAGATCAAACTTATACCCGGAGCGAAGCCGCCCGCAAAGAATGTTTATTGTATGGCACCACCAGAGCTGGCTGAACTTCGAAAACAGTTAGACGAGTTGCTGAGTACAGGGTTCATCAGGCCTGCAAAAGCTCCATATGGGGCCCCGGTTCTTTTCCAAAAGAAGAAGGATGGGAGCTTACGTCTATGTATCGACTACTGTGCATTGAATAAGCTCATAGTTCGCAACAAATACCTGTTCCCCATAATCACGAATTTATTTGACCGCCTACATGGAACGAAGTATTTCTCAAAATTGGATCTGCGATCGGGATACTATCAAGTAAGGATTGTCGAAGGGGACGAGCCAAAGACAACATGTGTCACCAGGTACGGAGCATTCGAATTCCTCGTAATGCCCTTTGGTCTCACCAATGCCCCTGCAACCTTTTGTATACTAATGAACCAAGTCTTCCATGA

mRNA sequence

ATGCCCGATAGTTTGCCCAAATTTCTACCGCCGAGAAGGATAATTGACCACGAGATCAAACTTATACCCGGAGCGAAGCCGCCCGCAAAGAATGTTTATTGTATGGCACCACCAGAGCTGGCTGAACTTCGAAAACAGTTAGACGAGTTGCTGAGTACAGGGTTCATCAGGCCTGCAAAAGCTCCATATGGGGCCCCGGTTCTTTTCCAAAAGAAGAAGGATGGGAGCTTACGTCTATTATTTCTCAAAATTGGATCTGCGATCGGGATACTATCAAGTAAGGATTGTCGAAGGGGACGAGCCAAAGACAACATGTGTCACCAGGTACGGAGCATTCGAATTCCTCGTAATGCCCTTTGGTCTCACCAATGCCCCTGCAACCTTTTGTATACTAATGAACCAAGTCTTCCATGA

Coding sequence (CDS)

ATGCCCGATAGTTTGCCCAAATTTCTACCGCCGAGAAGGATAATTGACCACGAGATCAAACTTATACCCGGAGCGAAGCCGCCCGCAAAGAATGTTTATTGTATGGCACCACCAGAGCTGGCTGAACTTCGAAAACAGTTAGACGAGTTGCTGAGTACAGGGTTCATCAGGCCTGCAAAAGCTCCATATGGGGCCCCGGTTCTTTTCCAAAAGAAGAAGGATGGGAGCTTACGTCTATTATTTCTCAAAATTGGATCTGCGATCGGGATACTATCAAGTAAGGATTGTCGAAGGGGACGAGCCAAAGACAACATGTGTCACCAGGTACGGAGCATTCGAATTCCTCGTAATGCCCTTTGGTCTCACCAATGCCCCTGCAACCTTTTGTATACTAATGAACCAAGTCTTCCATGA

Protein sequence

MPDSLPKFLPPRRIIDHEIKLIPGAKPPAKNVYCMAPPELAELRKQLDELLSTGFIRPAKAPYGAPVLFQKKKDGSLRLLFLKIGSAIGILSSKDCRRGRAKDNMCHQVRSIRIPRNALWSHQCPCNLLYTNEPSLP

Homology

[Close]

BLAST of PI0005478 vs. ExPASy Swiss-Prot
Match: Q99315 (Transposon Ty3-G Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY3B-G PE=1 SV=3)

HSP 1 Score: 52.8 bits (125), Expect = 3.7e-06
Identity = 28/77 (36.36%), Postives = 42/77 (54.55%), Query Frame = 0


Query: 9   LPPRRI------IDHEIKLIPGAKPPAKNVYCMAPPELAELRKQLDELLSTGFIRPAKAP 68
           LPPR        + H+I++ PGA+ P    Y +      E+ K + +LL   FI P+K+P
Sbjct: 572 LPPRPADINNIPVKHDIEIKPGARLPRLQPYHVTEKNEQEINKIVQKLLDNKFIVPSKSP 631

Query: 69  YGAPVLFQKKKDGSLRL 80
             +PV+   KKDG+ RL
Sbjct: 632 CSSPVVLVPKKDGTFRL 648

[Close]

BLAST of PI0005478 vs. ExPASy Swiss-Prot
Match: Q7LHG5 (Transposon Ty3-I Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY3B-I PE=1 SV=2)

HSP 1 Score: 52.8 bits (125), Expect = 3.7e-06
Identity = 28/77 (36.36%), Postives = 42/77 (54.55%), Query Frame = 0


Query: 9   LPPRRI------IDHEIKLIPGAKPPAKNVYCMAPPELAELRKQLDELLSTGFIRPAKAP 68
           LPPR        + H+I++ PGA+ P    Y +      E+ K + +LL   FI P+K+P
Sbjct: 598 LPPRPADINNIPVKHDIEIKPGARLPRLQPYHVTEKNEQEINKIVQKLLDNKFIVPSKSP 657

Query: 69  YGAPVLFQKKKDGSLRL 80
             +PV+   KKDG+ RL
Sbjct: 658 CSSPVVLVPKKDGTFRL 674

[Close]

BLAST of PI0005478 vs. ExPASy TrEMBL
Match: A0A5A7UQ94 (Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold280G001210 PE=4 SV=1)

HSP 1 Score: 153.3 bits (386), Expect = 7.4e-34
Identity = 71/79 (89.87%), Postives = 75/79 (94.94%), Query Frame = 0


Query: 1   MPDSLPKFLPPRRIIDHEIKLIPGAKPPAKNVYCMAPPELAELRKQLDELLSTGFIRPAK 60
           MPDSLPK LPPRR+IDHEI+L+PGAKPPAKN YCMAPPELAELRKQLDELL+ GFIRPAK
Sbjct: 528 MPDSLPKSLPPRRMIDHEIELVPGAKPPAKNAYCMAPPELAELRKQLDELLNAGFIRPAK 587

Query: 61  APYGAPVLFQKKKDGSLRL 80
           APYGAPVLFQKKKDG LRL
Sbjct: 588 APYGAPVLFQKKKDGRLRL 606

[Close]

BLAST of PI0005478 vs. ExPASy TrEMBL
Match: A0A5A7V6G0 (Retrotrans_gag domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold43900G00020 PE=4 SV=1)

HSP 1 Score: 151.4 bits (381), Expect = 2.8e-33
Identity = 72/79 (91.14%), Postives = 75/79 (94.94%), Query Frame = 0


Query: 1   MPDSLPKFLPPRRIIDHEIKLIPGAKPPAKNVYCMAPPELAELRKQLDELLSTGFIRPAK 60
           MPDSLPK LPPRR+IDHEI+L+PGAKPPAKN Y MAPPELAELRKQLDELLS GFIRPAK
Sbjct: 355 MPDSLPKSLPPRRMIDHEIELLPGAKPPAKNAYRMAPPELAELRKQLDELLSAGFIRPAK 414

Query: 61  APYGAPVLFQKKKDGSLRL 80
           APYGAPVLFQKKKDGSLRL
Sbjct: 415 APYGAPVLFQKKKDGSLRL 433

[Close]

BLAST of PI0005478 vs. ExPASy TrEMBL
Match: A0A5A7VHT2 (Reverse transcriptase domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold271G00020 PE=4 SV=1)

HSP 1 Score: 150.6 bits (379), Expect = 4.8e-33
Identity = 71/79 (89.87%), Postives = 75/79 (94.94%), Query Frame = 0


Query: 1   MPDSLPKFLPPRRIIDHEIKLIPGAKPPAKNVYCMAPPELAELRKQLDELLSTGFIRPAK 60
           MPDSLPK LPPRR+IDHEI+L+PGAKPPAKN Y MAPPELAELRKQLDELL+ GFIRPAK
Sbjct: 576 MPDSLPKSLPPRRMIDHEIELVPGAKPPAKNAYRMAPPELAELRKQLDELLNVGFIRPAK 635

Query: 61  APYGAPVLFQKKKDGSLRL 80
           APYGAPVLFQKKKDGSLRL
Sbjct: 636 APYGAPVLFQKKKDGSLRL 654

[Close]

BLAST of PI0005478 vs. ExPASy TrEMBL
Match: A0A5D3C9P8 (Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold265G00750 PE=4 SV=1)

HSP 1 Score: 150.6 bits (379), Expect = 4.8e-33
Identity = 71/79 (89.87%), Postives = 75/79 (94.94%), Query Frame = 0


Query: 1   MPDSLPKFLPPRRIIDHEIKLIPGAKPPAKNVYCMAPPELAELRKQLDELLSTGFIRPAK 60
           MPDSLPK LPPRR+IDHEI+L+PGAKPPAKN Y MAPPELAELRKQLDELL+ GFIRPAK
Sbjct: 610 MPDSLPKSLPPRRMIDHEIELVPGAKPPAKNAYRMAPPELAELRKQLDELLNAGFIRPAK 669

Query: 61  APYGAPVLFQKKKDGSLRL 80
           APYGAPVLFQKKKDGSLRL
Sbjct: 670 APYGAPVLFQKKKDGSLRL 688

[Close]

BLAST of PI0005478 vs. ExPASy TrEMBL
Match: A0A5D3DY42 (Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold165G00110 PE=4 SV=1)

HSP 1 Score: 150.6 bits (379), Expect = 4.8e-33
Identity = 71/79 (89.87%), Postives = 75/79 (94.94%), Query Frame = 0


Query: 1   MPDSLPKFLPPRRIIDHEIKLIPGAKPPAKNVYCMAPPELAELRKQLDELLSTGFIRPAK 60
           MPDSLPK LPPRR+IDHEI+L+PGAKPPAKN Y MAPPELAELRKQLDELL+ GFIRPAK
Sbjct: 96  MPDSLPKSLPPRRMIDHEIELVPGAKPPAKNAYRMAPPELAELRKQLDELLNAGFIRPAK 155

Query: 61  APYGAPVLFQKKKDGSLRL 80
           APYGAPVLFQKKKDGSLRL
Sbjct: 156 APYGAPVLFQKKKDGSLRL 174

[Close]

BLAST of PI0005478 vs. NCBI nr
Match: KAA0057280.1 (uncharacterized protein E6C27_scaffold280G001210 [Cucumis melo var. makuwa])

HSP 1 Score: 153.3 bits (386), Expect = 1.5e-33
Identity = 71/79 (89.87%), Postives = 75/79 (94.94%), Query Frame = 0


Query: 1   MPDSLPKFLPPRRIIDHEIKLIPGAKPPAKNVYCMAPPELAELRKQLDELLSTGFIRPAK 60
           MPDSLPK LPPRR+IDHEI+L+PGAKPPAKN YCMAPPELAELRKQLDELL+ GFIRPAK
Sbjct: 528 MPDSLPKSLPPRRMIDHEIELVPGAKPPAKNAYCMAPPELAELRKQLDELLNAGFIRPAK 587

Query: 61  APYGAPVLFQKKKDGSLRL 80
           APYGAPVLFQKKKDG LRL
Sbjct: 588 APYGAPVLFQKKKDGRLRL 606

[Close]

BLAST of PI0005478 vs. NCBI nr
Match: KAA0062527.1 (uncharacterized protein E6C27_scaffold43900G00020 [Cucumis melo var. makuwa])

HSP 1 Score: 151.4 bits (381), Expect = 5.8e-33
Identity = 72/79 (91.14%), Postives = 75/79 (94.94%), Query Frame = 0


Query: 1   MPDSLPKFLPPRRIIDHEIKLIPGAKPPAKNVYCMAPPELAELRKQLDELLSTGFIRPAK 60
           MPDSLPK LPPRR+IDHEI+L+PGAKPPAKN Y MAPPELAELRKQLDELLS GFIRPAK
Sbjct: 355 MPDSLPKSLPPRRMIDHEIELLPGAKPPAKNAYRMAPPELAELRKQLDELLSAGFIRPAK 414

Query: 61  APYGAPVLFQKKKDGSLRL 80
           APYGAPVLFQKKKDGSLRL
Sbjct: 415 APYGAPVLFQKKKDGSLRL 433

[Close]

BLAST of PI0005478 vs. NCBI nr
Match: TYK28653.1 (reverse transcriptase [Cucumis melo var. makuwa])

HSP 1 Score: 150.6 bits (379), Expect = 9.9e-33
Identity = 71/79 (89.87%), Postives = 75/79 (94.94%), Query Frame = 0


Query: 1   MPDSLPKFLPPRRIIDHEIKLIPGAKPPAKNVYCMAPPELAELRKQLDELLSTGFIRPAK 60
           MPDSLPK LPPRR+IDHEI+L+PGAKPPAKN Y MAPPELAELRKQLDELL+ GFIRPAK
Sbjct: 96  MPDSLPKSLPPRRMIDHEIELVPGAKPPAKNAYRMAPPELAELRKQLDELLNAGFIRPAK 155

Query: 61  APYGAPVLFQKKKDGSLRL 80
           APYGAPVLFQKKKDGSLRL
Sbjct: 156 APYGAPVLFQKKKDGSLRL 174

[Close]

BLAST of PI0005478 vs. NCBI nr
Match: TYK07954.1 (reverse transcriptase [Cucumis melo var. makuwa])

HSP 1 Score: 150.6 bits (379), Expect = 9.9e-33
Identity = 71/79 (89.87%), Postives = 75/79 (94.94%), Query Frame = 0


Query: 1   MPDSLPKFLPPRRIIDHEIKLIPGAKPPAKNVYCMAPPELAELRKQLDELLSTGFIRPAK 60
           MPDSLPK LPPRR+IDHEI+L+PGAKPPAKN Y MAPPELAELRKQLDELL+ GFIRPAK
Sbjct: 610 MPDSLPKSLPPRRMIDHEIELVPGAKPPAKNAYRMAPPELAELRKQLDELLNAGFIRPAK 669

Query: 61  APYGAPVLFQKKKDGSLRL 80
           APYGAPVLFQKKKDGSLRL
Sbjct: 670 APYGAPVLFQKKKDGSLRL 688

[Close]

BLAST of PI0005478 vs. NCBI nr
Match: KAA0066677.1 (uncharacterized protein E6C27_scaffold271G00020 [Cucumis melo var. makuwa])

HSP 1 Score: 150.6 bits (379), Expect = 9.9e-33
Identity = 71/79 (89.87%), Postives = 75/79 (94.94%), Query Frame = 0


Query: 1   MPDSLPKFLPPRRIIDHEIKLIPGAKPPAKNVYCMAPPELAELRKQLDELLSTGFIRPAK 60
           MPDSLPK LPPRR+IDHEI+L+PGAKPPAKN Y MAPPELAELRKQLDELL+ GFIRPAK
Sbjct: 576 MPDSLPKSLPPRRMIDHEIELVPGAKPPAKNAYRMAPPELAELRKQLDELLNVGFIRPAK 635

Query: 61  APYGAPVLFQKKKDGSLRL 80
           APYGAPVLFQKKKDGSLRL
Sbjct: 636 APYGAPVLFQKKKDGSLRL 654

The following BLAST results are available for this feature:

BLAST of PI0005478 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q99315	3.7e-06	36.36	Transposon Ty3-G Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 20...	[more]
Q7LHG5	3.7e-06	36.36	Transposon Ty3-I Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 20...	[more]

BLAST of PI0005478 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7UQ94	7.4e-34	89.87	Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold...	[more]
A0A5A7V6G0	2.8e-33	91.14	Retrotrans_gag domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 ...	[more]
A0A5A7VHT2	4.8e-33	89.87	Reverse transcriptase domain-containing protein OS=Cucumis melo var. makuwa OX=1...	[more]
A0A5D3C9P8	4.8e-33	89.87	Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold26...	[more]
A0A5D3DY42	4.8e-33	89.87	Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16...	[more]

BLAST of PI0005478 vs. NCBI nr


Match Name	E-value	Identity	Description
KAA0057280.1	1.5e-33	89.87	uncharacterized protein E6C27_scaffold280G001210 [Cucumis melo var. makuwa]	[more]
KAA0062527.1	5.8e-33	91.14	uncharacterized protein E6C27_scaffold43900G00020 [Cucumis melo var. makuwa]	[more]
TYK28653.1	9.9e-33	89.87	reverse transcriptase [Cucumis melo var. makuwa]	[more]
TYK07954.1	9.9e-33	89.87	reverse transcriptase [Cucumis melo var. makuwa]	[more]
KAA0066677.1	9.9e-33	89.87	uncharacterized protein E6C27_scaffold271G00020 [Cucumis melo var. makuwa]	[more]

BLAST of PI0005478 vs. NCBI nr


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.10.10.10	HIV Type 1 Reverse Transcriptase, subunit A, domain 1	coord: 8..115 e-value: 2.5E-18 score: 68.3
None	No IPR available	PANTHER	PTHR24559	TRANSPOSON TY3-I GAG-POL POLYPROTEIN	coord: 2..102
None	No IPR available	PANTHER	PTHR24559:SF330	RNA-DIRECTED DNA POLYMERASE HOMOLOG	coord: 2..102
IPR043502	DNA/RNA polymerase superfamily	SUPERFAMILY	56672	DNA/RNA polymerases	coord: 5..80

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
PI0005478.1	PI0005478.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0015074	DNA integration
biological_process	GO:0006278	RNA-dependent DNA biosynthetic process
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0003964	RNA-directed DNA polymerase activity