PI0005161 (gene) Melon (PI 482460) v1

Overview


Name	PI0005161
Type	gene
Organism	Cucumis metuliferus (Melon (PI 482460) v1)
Description	meiotic recombination protein SPO11-1
Location	chr01: 13174943 .. 13178656 (+)
RNA-Seq Expression	PI0005161
Synteny	PI0005161

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGGGAAACCGAGGAAGCTTGGACAGAATTGAAGTTCTGAGAAAAATCACAGGTCACAATTCCATTGATTTTTCTGATTCTCATATTTATTCCAATTGATTTCTTTTTCGTCGTTGCTATTTTCTTTCCGTTTTCCAATTCAACGTTTTGTAGAGCTAACGAGATCTATGATCGTCGAACTGAGCAATGGAAGCTCCAAGATGATCGCCATCGATCGGTTCAAAAATTACTGCACCGATTCCCTTGAAAACTGGTTCGTAATTTTCTGTGTAGAATATTTTGCTTCCAATATTGATTTCTTTTGAATCTTCGAATTGGTAACCAAATAAAGTTCATGATCTACAATGTTTCCAAGTGATTTGCTCGACTACGAATATTCATTTCCCTATTCGTATGACAATTTTCGATAGTTTCCGATTCTTTATTTACCATCGGGTCTATTGGATCGTATCGAGGCCAAACCAGTAGTCCCTCACAAAATCCAGTAGAAAATCAGTGTTAGTTCAGATGCGAATATATCTCTAGAACTTGAAGCACAGTTGCATAGTTTAGCTATGATTTGTCATTTTCGAAAGTCACTGCTCTATCTTAATTCTTAACTCAAATGCATTTAATGTTTATGGCCTAGTTTTCAGTCATTCTTTCGTTTTTTTGATATAATATTTTTATTTTTCAATAAAATTTAGTTCTCGAATGGGAAGATGACACTATCTTGGAATGGACTTGTGTTTTGAAAACGATATTTGTACCAAAAGAGATGTTTAGATGATTTTTCGCTAATTTTTTATGCAGTTGTTGTAGGTTCAATTTATCTGAAGGGAAGGAGATTCTAACACTTCAAAGGGAAAGCCATGTCCGTAGACTAGGTGATTTAGTTATTCCGATGAATGATACTACAAAATCATAATCTGTATAGACTATAGAGTACACTTGTATTTGATATTATTTTGTTCTATCAGATGTCTTGCTTAGAGTTCTACTCATAATTCAGCAGCTTCTGCGAGACAATAGACATAGCTCAAAGAGAGATATATATTATATGCATCCCTCTATATTTACAGGTAATCGAACCCACAATTATGTTCTAAGATACAACATCTTACAACAATTAATTGGCAGGCACGTTCAGTAAATTCGAATTTCAACATGTCCTTCAAATTTATGATTTCAGCAAGTTTGTGGCAGGAGATTTTCTGAACATTTCCTTGTTTTGCTTGGTTATGGTACAACAGAGTTTAATGACATTGGCTTCCTCATATAATGATGAATATTTTTCAGACCAATCAGTTGTTGACCGGGCAATCAATGACATCTGCATTCTTTTGCAATGTAGCCGGCATAATCTGAATGTGGTTGGTGCTGCACTTCATTTTACTTTGGCTCCAATTTACATATCTTCTTTTCCTCCATGATTATATCGGTTCATGTGGGATCCAATCTCAGTATAGCATTTAGGGTTGGCTGCGTGCCACAAAATGCTGTTCTTTGATAAAAGAAATGCATTATCGTTTCTTGTTGTCTTGCATCACTATTAGGTGTTGAACTAGGAAGATACTCTTCAGCCGATCAATTTCATTTTTCTTTAAATCATATAGTTTTTGTCTGTTTCTGATGATCATTTAAACTTGCGTTCTCTCAGGTTTCTGTTGGTAATGGGTAAGTATCGAGGAAAACATATGCTCAGTTCAGATAAGAAGAATATAGTGCTGAATGCTGCTTGCTTTCTTGACTTAATCCTTTACAAGCAGTCTGTAATTCATAATTATTTGTTTTGTGGGTTGATACCCATGTCAGGCTTTTCTATACCATTAGATTCTGTTAAATTTCCTCTTAGTGCTGCAATTTGTTTTCCTTGTGGAAGAGAATTAAGAACTGAATCTTGACATTTTCATTCAGGAAGAAATTTAAGAACTGAATCTTGACATTTTCATTGAGGAAGAAATTTAAGAACTGAATCTTGACATAGTCACTTTTTTTTTTTATAGGTTGGTGATGGGTTGGTTGAGGTTCTCAGAAGCTGGCAGAAGATTTGATTGCTTGAAGAAGCCTAACAGTGTAAACATTATTATTCTTCACAATACAATTGCCTCTTTAGTCACTCTCAGCTGTAACTGATTTGCATGCATGCATCTCAATCTCTTACAGGCCCACTTTATTCCTGTTCAAGTTGAAGAAGTTACAGGTATGCTGTCTGCTGATACTATATATAACTTAGCCAATCTCCTAGATTAGAGCCTAAATTAGACGCTTGGAATGATACCTTTAAATTACATTGTTCTTATGGCATTCCTTTCCTGCATATTCTTGAAAGAAAAATCCTTAAGTTCATTTTAGATGTCACCTATTTCTGTGTATTCCGACAAATTGTCTAATCATAATTTGTCACAGGACAAACTCTTCCTACATTTTTTAAAATATTTTAATTAATTTTTATGTTATGTAATGAGAGTGGGATGCATCTTTGGATGGATCCAAGTATTACTCTTTTCGAATGCATTTTGACTGAGTTATGCTTTCTCTATGATTATTTTTTATTTTTATATGCAGATATTGCTAGTGCTGCAGACTACATACTCGTTGTAGAGAAAGAATCAGGTCAAGACTATAGTGATAAAAACTGGTTTCACTCTCAAGGAAGCTCAATATTAACTTTCACTTTGATCATTTAAAGTATCTGGTGTTTCATTTTTTGAGCGTGCAGTTTTTCAGCGTTTAGCAAATGACCGGTTCTGCAGCAGAAACCGGTGCATTGTAATCACGGTGAGCATGCCTCAAGTCCTACAAACTCGAAAGTTCTCATATTTAAATGCTAGCTTTTTTCTTTTAGGGACGTGGATATCCAGATGTTCCCACTAGGAGGTAAAGAAATTATGGTCATGAACATGCTAATCTCATTTTTTAATTGATGATGTGAGTCATTGAAATATGCACACATGTATTGATCAGGTTTTTGCGGCTTCTCGTTGATGTTTTGGCATTGCCTGCATTTTGCTTGGTAGATTGTGATCCATATGGTTTTGACATTCTGACGACATATCGATTTGGTTCTATGGTTAGGCTCCTCAATTAAACAATTATCTAAATTTATTATCTACTAAATTATTTAATGCGAACTGCAAACTAACTAGTTGTATTATGTGTGAAACAATGAGAGCATGATAACTCGCTTTCCCTTCAATGTTTTTGATCCATTTCCTTTTCGAAAGCAAATGGCCTACGATGCAAAGTATCTCAGAATCCCACAACTACAATGGCTTGGAGCTTTCGCTTCAGATTCTGAGAAATACAATCTACCAGAACAATGTCTCATCCCATTGACTTTCAAGGGTGCGTGATCTCACATGAACGCAGATGCACACACGTACACTGTATTTTCAAATGTCATTTTCTGTATTTCTGATCTTTTGAAGGTCTTGTTTAACCTGTCAGACAAGAGAAGAACTGAAGCCTTGTTACAAAGATGCTACTTGCGGCGTGAGGTACCACATTGGAGGTAAAATTTTACCATTACAGCTCTAGTTTTCTATCATTGTTTCATGCATTGCCATTTTCTACAAAGGGTACTTCCCCCTTAGGTTGGAATTGGACTCGATGCTGGAAAGAGGGGTGAAATTTGAGATAGAAGCACTATCTGCGCACAATATTTCATTTTTGTCAGAGGAATATTTACCATCCAAAATAGAAAGTGAACTAACAACTGGTAAACTCTAG

mRNA sequence

ATGGAGGGAAACCGAGGAAGCTTGGACAGAATTGAAGTTCTGAGAAAAATCACAGAGCTAACGAGATCTATGATCGTCGAACTGAGCAATGGAAGCTCCAAGATGATCGCCATCGATCGGTTCAAAAATTACTGCACCGATTCCCTTGAAAACTGTTGTTGTAGGTTCAATTTATCTGAAGGGAAGGAGATTCTAACACTTCAAAGGGAAAGCCATGTCCGTAGACTAGATGTCTTGCTTAGAGTTCTACTCATAATTCAGCAGCTTCTGCGAGACAATAGACATAGCTCAAAGAGAGATATATATTATATGCATCCCTCTATATTTACAGACCAATCAGTTGTTGACCGGGCAATCAATGACATCTGCATTCTTTTGCAATGTAGCCGGCATAATCTGAATGTGGTTTCTGTTGGTAATGGGTTGGTGATGGGTTGGTTGAGGTTCTCAGAAGCTGGCAGAAGATTTGATTGCTTGAAGAAGCCTAACAGTGCCCACTTTATTCCTGTTCAAGTTGAAGAAGTTACAGATATTGCTAGTGCTGCAGACTACATACTCGTTGTAGAGAAAGAATCAGTTTTTCAGCGTTTAGCAAATGACCGGTTCTGCAGCAGAAACCGGTGCATTGTAATCACGGGACGTGGATATCCAGATGTTCCCACTAGGAGGTTTTTGCGGCTTCTCGTTGATGTTTTGGCATTGCCTGCATTTTGCTTGGTAGATTGTGATCCATATGGTTTTGACATTCTGACGACATATCGATTTGGTTCTATGCAAATGGCCTACGATGCAAAGTATCTCAGAATCCCACAACTACAATGGCTTGGAGCTTTCGCTTCAGATTCTGAGAAATACAATCTACCAGAACAATGTCTCATCCCATTGACTTTCAAGGACAAGAGAAGAACTGAAGCCTTGTTACAAAGATGCTACTTGCGGCGTGAGGTACCACATTGGAGGTTGGAATTGGACTCGATGCTGGAAAGAGGGGTGAAATTTGAGATAGAAGCACTATCTGCGCACAATATTTCATTTTTGTCAGAGGAATATTTACCATCCAAAATAGAAAGTGAACTAACAACTGGTAAACTCTAG

Coding sequence (CDS)

ATGGAGGGAAACCGAGGAAGCTTGGACAGAATTGAAGTTCTGAGAAAAATCACAGAGCTAACGAGATCTATGATCGTCGAACTGAGCAATGGAAGCTCCAAGATGATCGCCATCGATCGGTTCAAAAATTACTGCACCGATTCCCTTGAAAACTGTTGTTGTAGGTTCAATTTATCTGAAGGGAAGGAGATTCTAACACTTCAAAGGGAAAGCCATGTCCGTAGACTAGATGTCTTGCTTAGAGTTCTACTCATAATTCAGCAGCTTCTGCGAGACAATAGACATAGCTCAAAGAGAGATATATATTATATGCATCCCTCTATATTTACAGACCAATCAGTTGTTGACCGGGCAATCAATGACATCTGCATTCTTTTGCAATGTAGCCGGCATAATCTGAATGTGGTTTCTGTTGGTAATGGGTTGGTGATGGGTTGGTTGAGGTTCTCAGAAGCTGGCAGAAGATTTGATTGCTTGAAGAAGCCTAACAGTGCCCACTTTATTCCTGTTCAAGTTGAAGAAGTTACAGATATTGCTAGTGCTGCAGACTACATACTCGTTGTAGAGAAAGAATCAGTTTTTCAGCGTTTAGCAAATGACCGGTTCTGCAGCAGAAACCGGTGCATTGTAATCACGGGACGTGGATATCCAGATGTTCCCACTAGGAGGTTTTTGCGGCTTCTCGTTGATGTTTTGGCATTGCCTGCATTTTGCTTGGTAGATTGTGATCCATATGGTTTTGACATTCTGACGACATATCGATTTGGTTCTATGCAAATGGCCTACGATGCAAAGTATCTCAGAATCCCACAACTACAATGGCTTGGAGCTTTCGCTTCAGATTCTGAGAAATACAATCTACCAGAACAATGTCTCATCCCATTGACTTTCAAGGACAAGAGAAGAACTGAAGCCTTGTTACAAAGATGCTACTTGCGGCGTGAGGTACCACATTGGAGGTTGGAATTGGACTCGATGCTGGAAAGAGGGGTGAAATTTGAGATAGAAGCACTATCTGCGCACAATATTTCATTTTTGTCAGAGGAATATTTACCATCCAAAATAGAAAGTGAACTAACAACTGGTAAACTCTAG

Protein sequence

MEGNRGSLDRIEVLRKITELTRSMIVELSNGSSKMIAIDRFKNYCTDSLENCCCRFNLSEGKEILTLQRESHVRRLDVLLRVLLIIQQLLRDNRHSSKRDIYYMHPSIFTDQSVVDRAINDICILLQCSRHNLNVVSVGNGLVMGWLRFSEAGRRFDCLKKPNSAHFIPVQVEEVTDIASAADYILVVEKESVFQRLANDRFCSRNRCIVITGRGYPDVPTRRFLRLLVDVLALPAFCLVDCDPYGFDILTTYRFGSMQMAYDAKYLRIPQLQWLGAFASDSEKYNLPEQCLIPLTFKDKRRTEALLQRCYLRREVPHWRLELDSMLERGVKFEIEALSAHNISFLSEEYLPSKIESELTTGKL

Homology

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BLAST of PI0005161 vs. ExPASy Swiss-Prot
Match: Q9M4A2 (Meiotic recombination protein SPO11-1 OS=Arabidopsis thaliana OX=3702 GN=SPO11-1 PE=1 SV=1)

HSP 1 Score: 498.4 bits (1282), Expect = 6.7e-140
Identity = 237/361 (65.65%), Postives = 297/361 (82.27%), Query Frame = 0


Query: 1   MEGNRGSLDRIEVLRKITELTRSMIVELSNGSSKMIAIDRFKNYCTDSLENCCCRFNLSE 60
           MEG     +   +L++I + T+S++V+L+ G S  I+I++F+NYC +   +C C  +  +
Sbjct: 1   MEGKFAISESTNLLQRIKDFTQSVVVDLAEGRSPKISINQFRNYCMNPEADCLCSSDKPK 60

Query: 61  GKEILTLQRESHVRRLDVLLRVLLIIQQLLRDNRHSSKRDIYYMHPSIFTDQSVVDRAIN 120
           G+EI TL++E    R+D+LLRVLLI+QQLL++NRH+SKRDIYYMHPS F  QS+VDRAI 
Sbjct: 61  GQEIFTLKKEPQTYRIDMLLRVLLIVQQLLQENRHASKRDIYYMHPSAFKAQSIVDRAIG 120

Query: 121 DICILLQCSRHNLNVVSVGNGLVMGWLRFSEAGRRFDCLKKPNSAHFIPVQVEEVTDIAS 180
           DICIL QCSR+NLNVVSVGNGLVMGWL+F EAGR+FDCL   N+A+ +PV VEEV DI S
Sbjct: 121 DICILFQCSRYNLNVVSVGNGLVMGWLKFREAGRKFDCLNSLNTAYPVPVLVEEVEDIVS 180

Query: 181 AADYILVVEKESVFQRLANDRFCSRNRCIVITGRGYPDVPTRRFLRLLVDVLALPAFCLV 240
            A+YILVVEKE+VFQRLAND FC  NRCIVITGRGYPDV TRRFLRLL++ L LP  CLV
Sbjct: 181 LAEYILVVEKETVFQRLANDMFCKTNRCIVITGRGYPDVSTRRFLRLLMEKLHLPVHCLV 240

Query: 241 DCDPYGFDILTTYRFGSMQMAYDAKYLRIPQLQWLGAFASDSEKYNLPEQCLIPLTFKDK 300
           DCDPYGF+IL TYRFGSMQMAYD + LR P ++WLGAF SDSE Y++P+QCL+PLT +DK
Sbjct: 241 DCDPYGFEILATYRFGSMQMAYDIESLRAPDMKWLGAFPSDSEVYSVPKQCLLPLTEEDK 300

Query: 301 RRTEALLQRCYLRREVPHWRLELDSMLERGVKFEIEALSAHNISFLSEEYLPSKIESELT 360
           +RTEA+L RCYL+RE+P WRLEL++ML+RGVKFEIEALS H++SFLSE Y+PSKI  E++
Sbjct: 301 KRTEAMLLRCYLKREMPQWRLELETMLKRGVKFEIEALSVHSLSFLSEVYIPSKIRREVS 360

Query: 361 T 362
           +
Sbjct: 361 S 361

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BLAST of PI0005161 vs. ExPASy Swiss-Prot
Match: Q5ZPV8 (Meiotic recombination protein SPO11-1 OS=Oryza sativa subsp. indica OX=39946 GN=SPO11-1 PE=2 SV=1)

HSP 1 Score: 449.9 bits (1156), Expect = 2.7e-125
Identity = 212/360 (58.89%), Postives = 275/360 (76.39%), Query Frame = 0


Query: 2   EGNRGSLDRIEVLRKITELTRSMIVELSNGSSKMIAIDRFKNYCTD----SLENCCCRFN 61
           E  R   +   +L +I  L R ++ E++ G S  +A+ R++NYC+     +   C C ++
Sbjct: 17  ERRRRQEEAATLLHRIRGLVRWVVAEVAAGRSPTVALHRYQNYCSSASAAAASPCACSYD 76

Query: 62  LSEGKEILT-LQRESHVRRLDVLLRVLLIIQQLLRDNRHSSKRDIYYMHPSIFTDQSVVD 121
           +  G ++L+ L R SH  RL+VLLRVLL++QQLL+ N+H SKRDIYYM+PSIF +Q+VVD
Sbjct: 77  VPVGTDVLSLLHRGSHASRLNVLLRVLLVVQQLLQQNKHCSKRDIYYMYPSIFQEQAVVD 136

Query: 122 RAINDICILLQCSRHNLNVVSVGNGLVMGWLRFSEAGRRFDCLKKPNSAHFIPVQVEEVT 181
           RAINDIC+L +CSRHNLNVV V  GLVMGW+RF E  +   C+   N+A  IPV +E + 
Sbjct: 137 RAINDICVLFKCSRHNLNVVPVAKGLVMGWIRFLEGEKEVYCVTNVNAAFSIPVSIEAIK 196

Query: 182 DIASAADYILVVEKESVFQRLANDRFCSRNRCIVITGRGYPDVPTRRFLRLLVDVLALPA 241
           D+ S ADYIL+VEKE+VFQRLAND+FC RNRCIVITGRGYPD+PTRRFLR LV+ L LP 
Sbjct: 197 DVVSVADYILIVEKETVFQRLANDKFCERNRCIVITGRGYPDIPTRRFLRYLVEQLHLPV 256

Query: 242 FCLVDCDPYGFDILTTYRFGSMQMAYDAKYLRIPQLQWLGAFASDSEKYNLPEQCLIPLT 301
           +CLVD DPYGFDIL TY+FGS+Q+AYDA +LR+P ++WLG F SD E Y LP+ CL+ L+
Sbjct: 257 YCLVDADPYGFDILATYKFGSLQLAYDANFLRVPDIRWLGVFTSDFEDYRLPDCCLLHLS 316

Query: 302 FKDKRRTEALLQRCYLRREVPHWRLELDSMLERGVKFEIEALSAHNISFLSEEYLPSKIE 357
            +D+R+ E +L RCYL RE P WRLEL++ML++GVKFEIEALSA +ISFLSEEY+P KI+
Sbjct: 317 SEDRRKAEGILSRCYLHREAPQWRLELEAMLQKGVKFEIEALSACSISFLSEEYIPKKIK 376

[Close]

BLAST of PI0005161 vs. ExPASy Swiss-Prot
Match: Q7Y021 (Meiotic recombination protein SPO11-1 OS=Oryza sativa subsp. japonica OX=39947 GN=SPO11-1 PE=2 SV=1)

HSP 1 Score: 449.9 bits (1156), Expect = 2.7e-125
Identity = 212/360 (58.89%), Postives = 275/360 (76.39%), Query Frame = 0


Query: 2   EGNRGSLDRIEVLRKITELTRSMIVELSNGSSKMIAIDRFKNYCTD----SLENCCCRFN 61
           E  R   +   +L +I  L R ++ E++ G S  +A+ R++NYC+     +   C C ++
Sbjct: 17  ERRRRQEEAATLLHRIRGLVRWVVAEVAAGRSPTVALHRYQNYCSSASAAAASPCACSYD 76

Query: 62  LSEGKEILT-LQRESHVRRLDVLLRVLLIIQQLLRDNRHSSKRDIYYMHPSIFTDQSVVD 121
           +  G ++L+ L R SH  RL+VLLRVLL++QQLL+ N+H SKRDIYYM+PSIF +Q+VVD
Sbjct: 77  VPVGTDVLSLLHRGSHASRLNVLLRVLLVVQQLLQQNKHCSKRDIYYMYPSIFQEQAVVD 136

Query: 122 RAINDICILLQCSRHNLNVVSVGNGLVMGWLRFSEAGRRFDCLKKPNSAHFIPVQVEEVT 181
           RAINDIC+L +CSRHNLNVV V  GLVMGW+RF E  +   C+   N+A  IPV +E + 
Sbjct: 137 RAINDICVLFKCSRHNLNVVPVAKGLVMGWIRFLEGEKEVYCVTNVNAAFSIPVSIEAIK 196

Query: 182 DIASAADYILVVEKESVFQRLANDRFCSRNRCIVITGRGYPDVPTRRFLRLLVDVLALPA 241
           D+ S ADYIL+VEKE+VFQRLAND+FC RNRCIVITGRGYPD+PTRRFLR LV+ L LP 
Sbjct: 197 DVVSVADYILIVEKETVFQRLANDKFCERNRCIVITGRGYPDIPTRRFLRYLVEQLHLPV 256

Query: 242 FCLVDCDPYGFDILTTYRFGSMQMAYDAKYLRIPQLQWLGAFASDSEKYNLPEQCLIPLT 301
           +CLVD DPYGFDIL TY+FGS+Q+AYDA +LR+P ++WLG F SD E Y LP+ CL+ L+
Sbjct: 257 YCLVDADPYGFDILATYKFGSLQLAYDANFLRVPDIRWLGVFTSDFEDYRLPDCCLLHLS 316

Query: 302 FKDKRRTEALLQRCYLRREVPHWRLELDSMLERGVKFEIEALSAHNISFLSEEYLPSKIE 357
            +D+R+ E +L RCYL RE P WRLEL++ML++GVKFEIEALSA +ISFLSEEY+P KI+
Sbjct: 317 SEDRRKAEGILSRCYLHREAPQWRLELEAMLQKGVKFEIEALSACSISFLSEEYIPKKIK 376

[Close]

BLAST of PI0005161 vs. ExPASy Swiss-Prot
Match: Q9LZ03 (DNA topoisomerase 6 subunit A OS=Arabidopsis thaliana OX=3702 GN=TOP6A PE=1 SV=1)

HSP 1 Score: 233.8 bits (595), Expect = 3.1e-60
Identity = 118/288 (40.97%), Postives = 178/288 (61.81%), Query Frame = 0


Query: 71  SHVRRLDVLLRVLLIIQQLLRDNRHSSKRDIYYMHPSIFTDQSVVDRAINDICILLQCSR 130
           S VR+  +  R+L +I QL   N H +KRD++Y    +F DQ+  D  ++D+  +L C+R
Sbjct: 138 SSVRKTTITTRILALIHQLCLRNIHVTKRDLFYTDVKLFQDQTQSDAVLDDVSCMLGCTR 197

Query: 131 HNLNVVSVGNGLVMGWLRFSEAGRRFDCLKKPNSAHFIPVQVEEVTDIASAADYILVVEK 190
            +LNV++   G+V+G L FS+ G   DC K       IP  ++ V D+ S A +IL+VEK
Sbjct: 198 SSLNVIAAEKGVVVGRLIFSDNGDMIDCTKMGMGGKAIPPNIDRVGDMQSDAMFILLVEK 257

Query: 191 ESVFQRLANDRFCSRNRCIVITGRGYPDVPTRRFLRLLVDVLALPAFCLVDCDPYGFDIL 250
           ++ + RLA DRF +R  CI++T +G PDV TR FLR +   L LP   LVD DPYG  IL
Sbjct: 258 DAAYMRLAEDRFYNRFPCIIVTAKGQPDVATRLFLRKMKMELKLPVLALVDSDPYGLKIL 317

Query: 251 TTYRFGSMQMAYDAKYLRIPQLQWLGAFASDSEKYNLPEQCLIPLTFKDKRRTEALLQRC 310
           + Y  GS  M+YD+  L  P ++WLG   SD +KY +PEQC +P+T +D +  + +L+  
Sbjct: 318 SVYGCGSKNMSYDSANLTTPDIKWLGIRPSDLDKYKIPEQCRLPMTEQDIKTGKDMLEED 377

Query: 311 YLRREVPHWRLELDSMLERGVKFEIEALSAHNISFLSEEYLPSKIESE 359
           ++++  P W  EL+ M++   K EI+ALS+    +LSE YLP K++ +
Sbjct: 378 FVKKN-PGWVEELNLMVKTKQKAEIQALSSFGFQYLSEVYLPLKLQQQ 424

[Close]

BLAST of PI0005161 vs. ExPASy Swiss-Prot
Match: Q9Y5K1 (Meiotic recombination protein SPO11 OS=Homo sapiens OX=9606 GN=SPO11 PE=1 SV=1)

HSP 1 Score: 229.2 bits (583), Expect = 7.6e-59
Identity = 123/355 (34.65%), Postives = 221/355 (62.25%), Query Frame = 0


Query: 12  EVLRKITELTRSMIVELSNGSSKMIAIDRFKNYCTDSLENCCCRFNLSEGKEILT----- 71
           EVL  I  + + +I  L+   +    ID        S EN   +F  S G ++++     
Sbjct: 46  EVLASIENIIQDIITSLARNEAPAFTIDN-----RSSWEN--IKFEDSVGLQMVSHCTTR 105

Query: 72  -LQRES--HVRRLDVLLRVLLIIQQLLRDNRHSSKRDIYYMHPSIFTDQSVVDRAINDIC 131
            ++ +S    ++  ++L++L +I +L++ N +++KRDIYY    +F +Q+VVD  INDI 
Sbjct: 106 KIKSDSPKSAQKFSLILKILSMIYKLVQSNTYATKRDIYYTDSQLFGNQTVVDNIINDIS 165

Query: 132 ILLQCSRHNLNVVSVGNGLVMGWLRF-SEAGRRFDCLKKPNSAHFIPVQVEEVTDIASAA 191
            +L+ SR +L+++S   GL+ G LR+  E G + +C     +A  +P  ++ + ++ + A
Sbjct: 166 CMLKVSRRSLHILSTSKGLIAGNLRYIEEDGTKVNCTCGA-TAVAVPSNIQGIRNLVTDA 225

Query: 192 DYILVVEKESVFQRLANDRFCSR-NRCIVITGRGYPDVPTRRFLRLLVDVLALPAFCLVD 251
            ++L+VEK++ FQRL +D FC++ + CI+ITG+G PD+ TR  ++ L D   +P F LVD
Sbjct: 226 KFVLIVEKDATFQRLLDDNFCNKLSPCIMITGKGVPDLNTRLLVKKLWDTFHVPVFTLVD 285

Query: 252 CDPYGFDILTTYRFGSMQMAYDAKYLRIPQLQWLGAFASDSEKYNLPEQCLIPLTFKDKR 311
            DP+G +I+  Y++GSM M+++A +L +P ++WLG   SD ++ N+P+  LIPLT +D+ 
Sbjct: 286 ADPHGIEIMCIYKYGSMSMSFEAHHLTVPAIRWLGLLPSDLKRLNVPKDSLIPLTKRDQM 345

Query: 312 RTEALLQRCYLRREVPHWRLELDSMLERGVKFEIEALSAHNISFLSEEYLPSKIE 357
           + +++L+R Y+  + P WR E++ M +  +K EI+AL+  +  +LS  YLP+K++
Sbjct: 346 KLDSILRRPYVTCQ-PFWRKEMEIMADSKMKAEIQALTFLSSDYLSRVYLPNKLK 391

[Close]

BLAST of PI0005161 vs. ExPASy TrEMBL
Match: A0A1S3CJR6 (meiotic recombination protein SPO11-1 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103501238 PE=3 SV=1)

HSP 1 Score: 703.4 bits (1814), Expect = 5.1e-199
Identity = 347/364 (95.33%), Postives = 359/364 (98.63%), Query Frame = 0


Query: 1   MEGNRGSLDRIEVLRKITELTRSMIVELSNGSSKMIAIDRFKNYCTDSLENCCCRFNLSE 60
           MEGNRGSL+RIEVLRKITELTRSMIVELSNGSSKMIAIDRFK+YCT+SLENCCCRFNLSE
Sbjct: 1   MEGNRGSLERIEVLRKITELTRSMIVELSNGSSKMIAIDRFKSYCTNSLENCCCRFNLSE 60

Query: 61  GKEILTLQRESHVRRLDVLLRVLLIIQQLLRDNRHSSKRDIYYMHPSIFTDQSVVDRAIN 120
           GK++LTLQRESHVRRLDVLLRVLLIIQQLLR+NRHSSKRD+YYMHPSIFTDQSVVDRAIN
Sbjct: 61  GKDVLTLQRESHVRRLDVLLRVLLIIQQLLRENRHSSKRDMYYMHPSIFTDQSVVDRAIN 120

Query: 121 DICILLQCSRHNLNVVSVGNGLVMGWLRFSEAGRRFDCLKKPNSAHFIPVQVEEVTDIAS 180
           DICILLQCSRHNLNVVSVGNGLVMGWLRFSEAGRRFDCLKKPNSA FIPVQVEEVTDIA+
Sbjct: 121 DICILLQCSRHNLNVVSVGNGLVMGWLRFSEAGRRFDCLKKPNSAQFIPVQVEEVTDIAT 180

Query: 181 AADYILVVEKESVFQRLANDRFCSRNRCIVITGRGYPDVPTRRFLRLLVDVLALPAFCLV 240
           AADYILVVEKESVFQRLANDRFCSRNRCIVITGRGYPDVPTRRFLRLLVDVLALPAFCLV
Sbjct: 181 AADYILVVEKESVFQRLANDRFCSRNRCIVITGRGYPDVPTRRFLRLLVDVLALPAFCLV 240

Query: 241 DCDPYGFDILTTYRFGSMQMAYDAKYLRIPQLQWLGAFASDSEKYNLPEQCLIPLTFKDK 300
           DCDPYGFDILTTYRFGSMQMAYDAK LRIPQLQW+GAFASDSEKY+LPEQCLIPLT KDK
Sbjct: 241 DCDPYGFDILTTYRFGSMQMAYDAKNLRIPQLQWIGAFASDSEKYDLPEQCLIPLTSKDK 300

Query: 301 RRTEALLQRCYLRREVPHWRLELDSMLERGVKFEIEALSAHNISFLSEEYLPSKIESELT 360
           RRTEALLQRCYLRREVPHWRLELDSML+RGVKFEIEALS HNISFLS+ YLPSKIESELT
Sbjct: 301 RRTEALLQRCYLRREVPHWRLELDSMLQRGVKFEIEALSVHNISFLSDHYLPSKIESELT 360

Query: 361 TGKL 365
           TGKL
Sbjct: 361 TGKL 364

[Close]

BLAST of PI0005161 vs. ExPASy TrEMBL
Match: A0A1S3CI64 (meiotic recombination protein SPO11-1 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103501238 PE=3 SV=1)

HSP 1 Score: 685.6 bits (1768), Expect = 1.1e-193
Identity = 343/364 (94.23%), Postives = 355/364 (97.53%), Query Frame = 0


Query: 1   MEGNRGSLDRIEVLRKITELTRSMIVELSNGSSKMIAIDRFKNYCTDSLENCCCRFNLSE 60
           MEGNRGSL+RIEVLRKITELTRSMIVELSNGSSKMIAIDRFK+YCT+SLEN    FNLSE
Sbjct: 1   MEGNRGSLERIEVLRKITELTRSMIVELSNGSSKMIAIDRFKSYCTNSLENW---FNLSE 60

Query: 61  GKEILTLQRESHVRRLDVLLRVLLIIQQLLRDNRHSSKRDIYYMHPSIFTDQSVVDRAIN 120
           GK++LTLQRESHVRRLDVLLRVLLIIQQLLR+NRHSSKRD+YYMHPSIFTDQSVVDRAIN
Sbjct: 61  GKDVLTLQRESHVRRLDVLLRVLLIIQQLLRENRHSSKRDMYYMHPSIFTDQSVVDRAIN 120

Query: 121 DICILLQCSRHNLNVVSVGNGLVMGWLRFSEAGRRFDCLKKPNSAHFIPVQVEEVTDIAS 180
           DICILLQCSRHNLNVVSVGNGLVMGWLRFSEAGRRFDCLKKPNSA FIPVQVEEVTDIA+
Sbjct: 121 DICILLQCSRHNLNVVSVGNGLVMGWLRFSEAGRRFDCLKKPNSAQFIPVQVEEVTDIAT 180

Query: 181 AADYILVVEKESVFQRLANDRFCSRNRCIVITGRGYPDVPTRRFLRLLVDVLALPAFCLV 240
           AADYILVVEKESVFQRLANDRFCSRNRCIVITGRGYPDVPTRRFLRLLVDVLALPAFCLV
Sbjct: 181 AADYILVVEKESVFQRLANDRFCSRNRCIVITGRGYPDVPTRRFLRLLVDVLALPAFCLV 240

Query: 241 DCDPYGFDILTTYRFGSMQMAYDAKYLRIPQLQWLGAFASDSEKYNLPEQCLIPLTFKDK 300
           DCDPYGFDILTTYRFGSMQMAYDAK LRIPQLQW+GAFASDSEKY+LPEQCLIPLT KDK
Sbjct: 241 DCDPYGFDILTTYRFGSMQMAYDAKNLRIPQLQWIGAFASDSEKYDLPEQCLIPLTSKDK 300

Query: 301 RRTEALLQRCYLRREVPHWRLELDSMLERGVKFEIEALSAHNISFLSEEYLPSKIESELT 360
           RRTEALLQRCYLRREVPHWRLELDSML+RGVKFEIEALS HNISFLS+ YLPSKIESELT
Sbjct: 301 RRTEALLQRCYLRREVPHWRLELDSMLQRGVKFEIEALSVHNISFLSDHYLPSKIESELT 360

Query: 361 TGKL 365
           TGKL
Sbjct: 361 TGKL 361

[Close]

BLAST of PI0005161 vs. ExPASy TrEMBL
Match: A0A6J1IGM0 (meiotic recombination protein SPO11-1 OS=Cucurbita maxima OX=3661 GN=LOC111476764 PE=3 SV=1)

HSP 1 Score: 673.3 bits (1736), Expect = 5.6e-190
Identity = 330/363 (90.91%), Postives = 347/363 (95.59%), Query Frame = 0


Query: 1   MEGNRGSLDRIEVLRKITELTRSMIVELSNGSSKMIAIDRFKNYCTDSLENCCCRFNLSE 60
           M GN G  +RI+++RKI ELTRSMIVELSNGSSKMIAIDRFKNYC +S+ENCCCRFNLSE
Sbjct: 1   MAGNLGRSERIDIMRKIRELTRSMIVELSNGSSKMIAIDRFKNYCINSIENCCCRFNLSE 60

Query: 61  GKEILTLQRESHVRRLDVLLRVLLIIQQLLRDNRHSSKRDIYYMHPSIFTDQSVVDRAIN 120
           GKEILTLQRESHVRRLDVLLRVLLIIQQLLR+NRHSSKRDIYYMHPS+FTDQSVVDRAIN
Sbjct: 61  GKEILTLQRESHVRRLDVLLRVLLIIQQLLRENRHSSKRDIYYMHPSVFTDQSVVDRAIN 120

Query: 121 DICILLQCSRHNLNVVSVGNGLVMGWLRFSEAGRRFDCLKKPNSAHFIPVQVEEVTDIAS 180
           DICILLQCSRHNLNVVSVGNGLVMGWLRF+EAGRR DC+KKPNSAH IPVQVEEVTDIAS
Sbjct: 121 DICILLQCSRHNLNVVSVGNGLVMGWLRFAEAGRRSDCIKKPNSAHSIPVQVEEVTDIAS 180

Query: 181 AADYILVVEKESVFQRLANDRFCSRNRCIVITGRGYPDVPTRRFLRLLVDVLALPAFCLV 240
            A+YILVVEKESVFQRLAND FCSRNRCIVI+GRGYPDVPTRRFLRLLVDVLALPAFCLV
Sbjct: 181 PANYILVVEKESVFQRLANDHFCSRNRCIVISGRGYPDVPTRRFLRLLVDVLALPAFCLV 240

Query: 241 DCDPYGFDILTTYRFGSMQMAYDAKYLRIPQLQWLGAFASDSEKYNLPEQCLIPLTFKDK 300
           DCDPYGFDILTTYRFGSMQMAYDAKYLRIPQL WLGAFASDS+ YNLP+QCLIPLT +DK
Sbjct: 241 DCDPYGFDILTTYRFGSMQMAYDAKYLRIPQLHWLGAFASDSDTYNLPQQCLIPLTLEDK 300

Query: 301 RRTEALLQRCYLRREVPHWRLELDSMLERGVKFEIEALSAHNISFLSEEYLPSKIESELT 360
           RRTEALL RCYLRREVPHWRLEL+SML+RGVKFEIEALS HNISFLSEEYLPSKIESELT
Sbjct: 301 RRTEALLLRCYLRREVPHWRLELESMLQRGVKFEIEALSVHNISFLSEEYLPSKIESELT 360

Query: 361 TGK 364
            GK
Sbjct: 361 KGK 363

[Close]

BLAST of PI0005161 vs. ExPASy TrEMBL
Match: A0A6J1F921 (meiotic recombination protein SPO11-1 OS=Cucurbita moschata OX=3662 GN=LOC111443423 PE=3 SV=1)

HSP 1 Score: 666.8 bits (1719), Expect = 5.3e-188
Identity = 328/365 (89.86%), Postives = 345/365 (94.52%), Query Frame = 0


Query: 1   MEGNRGSLD--RIEVLRKITELTRSMIVELSNGSSKMIAIDRFKNYCTDSLENCCCRFNL 60
           M G  GS +  RI+++RKI ELTRSMIVELSNGSSKMIAIDRFKNYCT+S ENCCCRFNL
Sbjct: 1   MAGKLGSSERIRIDIMRKIRELTRSMIVELSNGSSKMIAIDRFKNYCTNSTENCCCRFNL 60

Query: 61  SEGKEILTLQRESHVRRLDVLLRVLLIIQQLLRDNRHSSKRDIYYMHPSIFTDQSVVDRA 120
           SEGKEILTLQR+SHVRRLDVLLRVLLIIQQLLR+NRHSSKRDIYYMHPS+FTDQS VDRA
Sbjct: 61  SEGKEILTLQRQSHVRRLDVLLRVLLIIQQLLRENRHSSKRDIYYMHPSVFTDQSAVDRA 120

Query: 121 INDICILLQCSRHNLNVVSVGNGLVMGWLRFSEAGRRFDCLKKPNSAHFIPVQVEEVTDI 180
           INDICILLQCSRHNLNVVSVGNGLVMGWLRF+EAGRRFDC+KKPN+AH IPVQVEEVTDI
Sbjct: 121 INDICILLQCSRHNLNVVSVGNGLVMGWLRFAEAGRRFDCIKKPNNAHSIPVQVEEVTDI 180

Query: 181 ASAADYILVVEKESVFQRLANDRFCSRNRCIVITGRGYPDVPTRRFLRLLVDVLALPAFC 240
           A  A+YILVVEKESVFQRLAND FCSRNRCIVI+GRGYPDVPTRRFLRLLVDVLALPAFC
Sbjct: 181 AGPANYILVVEKESVFQRLANDHFCSRNRCIVISGRGYPDVPTRRFLRLLVDVLALPAFC 240

Query: 241 LVDCDPYGFDILTTYRFGSMQMAYDAKYLRIPQLQWLGAFASDSEKYNLPEQCLIPLTFK 300
           LVDCDPYGFDILTTYRFGSMQMAYDAKYLRIPQL WLGAFASDS  YNLP+QCLIPLT +
Sbjct: 241 LVDCDPYGFDILTTYRFGSMQMAYDAKYLRIPQLHWLGAFASDSNTYNLPQQCLIPLTLE 300

Query: 301 DKRRTEALLQRCYLRREVPHWRLELDSMLERGVKFEIEALSAHNISFLSEEYLPSKIESE 360
           DKRRTEALL RCYLRREVPHWRLEL+SML+RGVKFEIEALS HNISFLSEEYLPSKIESE
Sbjct: 301 DKRRTEALLPRCYLRREVPHWRLELESMLQRGVKFEIEALSVHNISFLSEEYLPSKIESE 360

Query: 361 LTTGK 364
           LT GK
Sbjct: 361 LTNGK 365

[Close]

BLAST of PI0005161 vs. ExPASy TrEMBL
Match: A0A6J1CDC7 (meiotic recombination protein SPO11-1 OS=Momordica charantia OX=3673 GN=LOC111010510 PE=3 SV=1)

HSP 1 Score: 657.1 bits (1694), Expect = 4.2e-185
Identity = 320/364 (87.91%), Postives = 345/364 (94.78%), Query Frame = 0


Query: 1   MEGNRGSLDRIEVLRKITELTRSMIVELSNGSSKMIAIDRFKNYCTDSLENCCCRFNLSE 60
           MEG R S +R ++LR+I E+TRSMIVELSNGSSKM+AIDRFKNYCT+SLENCCC+FNLSE
Sbjct: 1   MEGKRASSERSDILRRIREITRSMIVELSNGSSKMMAIDRFKNYCTNSLENCCCKFNLSE 60

Query: 61  GKEILTLQRESHVRRLDVLLRVLLIIQQLLRDNRHSSKRDIYYMHPSIFTDQSVVDRAIN 120
           GKEILTLQ+ESHVRRLDVLLRVLLI++QLLR+NRHSSKRDIYYMHPS+F+DQSVVDRAIN
Sbjct: 61  GKEILTLQKESHVRRLDVLLRVLLIVRQLLRENRHSSKRDIYYMHPSVFSDQSVVDRAIN 120

Query: 121 DICILLQCSRHNLNVVSVGNGLVMGWLRFSEAGRRFDCLKKPNSAHFIPVQVEEVTDIAS 180
           DICILLQCSRHNLNVVS GNGLVMGWLRFSEAGRRFDC+KKPNSAH IPVQVEEVTDI S
Sbjct: 121 DICILLQCSRHNLNVVSFGNGLVMGWLRFSEAGRRFDCIKKPNSAHSIPVQVEEVTDIVS 180

Query: 181 AADYILVVEKESVFQRLANDRFCSRNRCIVITGRGYPDVPTRRFLRLLVDVLALPAFCLV 240
           AA+YIL+VEKESVFQRLAND+FCSRNRCIVITGRGYPDVPTRRFLRLLVDV ALPAFCLV
Sbjct: 181 AAEYILIVEKESVFQRLANDQFCSRNRCIVITGRGYPDVPTRRFLRLLVDVSALPAFCLV 240

Query: 241 DCDPYGFDILTTYRFGSMQMAYDAKYLRIPQLQWLGAFASDSEKYNLPEQCLIPLTFKDK 300
           DCDPYGF+ILTTYRFGSMQMAYDAKYLRIP L+WLGAFASDSE YNLPEQCLIPLT +DK
Sbjct: 241 DCDPYGFEILTTYRFGSMQMAYDAKYLRIPHLRWLGAFASDSENYNLPEQCLIPLTMEDK 300

Query: 301 RRTEALLQRCYLRREVPHWRLELDSMLERGVKFEIEALSAHNISFLSEEYLPSKIES-EL 360
           RRTEALL RCYL+REVPHWRLELDSML++GVKFEIEALS HN+SFLSEEYLPSKI    L
Sbjct: 301 RRTEALLLRCYLQREVPHWRLELDSMLQKGVKFEIEALSVHNLSFLSEEYLPSKIGCVHL 360

Query: 361 TTGK 364
            TGK
Sbjct: 361 RTGK 364

[Close]

BLAST of PI0005161 vs. NCBI nr
Match: XP_008462989.1 (PREDICTED: meiotic recombination protein SPO11-1 isoform X1 [Cucumis melo])

HSP 1 Score: 703.4 bits (1814), Expect = 1.0e-198
Identity = 347/364 (95.33%), Postives = 359/364 (98.63%), Query Frame = 0


Query: 1   MEGNRGSLDRIEVLRKITELTRSMIVELSNGSSKMIAIDRFKNYCTDSLENCCCRFNLSE 60
           MEGNRGSL+RIEVLRKITELTRSMIVELSNGSSKMIAIDRFK+YCT+SLENCCCRFNLSE
Sbjct: 1   MEGNRGSLERIEVLRKITELTRSMIVELSNGSSKMIAIDRFKSYCTNSLENCCCRFNLSE 60

Query: 61  GKEILTLQRESHVRRLDVLLRVLLIIQQLLRDNRHSSKRDIYYMHPSIFTDQSVVDRAIN 120
           GK++LTLQRESHVRRLDVLLRVLLIIQQLLR+NRHSSKRD+YYMHPSIFTDQSVVDRAIN
Sbjct: 61  GKDVLTLQRESHVRRLDVLLRVLLIIQQLLRENRHSSKRDMYYMHPSIFTDQSVVDRAIN 120

Query: 121 DICILLQCSRHNLNVVSVGNGLVMGWLRFSEAGRRFDCLKKPNSAHFIPVQVEEVTDIAS 180
           DICILLQCSRHNLNVVSVGNGLVMGWLRFSEAGRRFDCLKKPNSA FIPVQVEEVTDIA+
Sbjct: 121 DICILLQCSRHNLNVVSVGNGLVMGWLRFSEAGRRFDCLKKPNSAQFIPVQVEEVTDIAT 180

Query: 181 AADYILVVEKESVFQRLANDRFCSRNRCIVITGRGYPDVPTRRFLRLLVDVLALPAFCLV 240
           AADYILVVEKESVFQRLANDRFCSRNRCIVITGRGYPDVPTRRFLRLLVDVLALPAFCLV
Sbjct: 181 AADYILVVEKESVFQRLANDRFCSRNRCIVITGRGYPDVPTRRFLRLLVDVLALPAFCLV 240

Query: 241 DCDPYGFDILTTYRFGSMQMAYDAKYLRIPQLQWLGAFASDSEKYNLPEQCLIPLTFKDK 300
           DCDPYGFDILTTYRFGSMQMAYDAK LRIPQLQW+GAFASDSEKY+LPEQCLIPLT KDK
Sbjct: 241 DCDPYGFDILTTYRFGSMQMAYDAKNLRIPQLQWIGAFASDSEKYDLPEQCLIPLTSKDK 300

Query: 301 RRTEALLQRCYLRREVPHWRLELDSMLERGVKFEIEALSAHNISFLSEEYLPSKIESELT 360
           RRTEALLQRCYLRREVPHWRLELDSML+RGVKFEIEALS HNISFLS+ YLPSKIESELT
Sbjct: 301 RRTEALLQRCYLRREVPHWRLELDSMLQRGVKFEIEALSVHNISFLSDHYLPSKIESELT 360

Query: 361 TGKL 365
           TGKL
Sbjct: 361 TGKL 364

[Close]

BLAST of PI0005161 vs. NCBI nr
Match: XP_031745985.1 (meiotic recombination protein SPO11-1-like isoform X3 [Cucumis sativus])

HSP 1 Score: 689.9 bits (1779), Expect = 1.2e-194
Identity = 339/360 (94.17%), Postives = 353/360 (98.06%), Query Frame = 0


Query: 1   MEGNRGSLDRIEVLRKITELTRSMIVELSNGSSKMIAIDRFKNYCTDSLENCCCRFNLSE 60
           MEGNRGSL+RIEV+RKITELTRSMIVELSNGSS+MIAIDRFK+YC++SLENCCCRFNLSE
Sbjct: 40  MEGNRGSLERIEVMRKITELTRSMIVELSNGSSRMIAIDRFKSYCSNSLENCCCRFNLSE 99

Query: 61  GKEILTLQRESHVRRLDVLLRVLLIIQQLLRDNRHSSKRDIYYMHPSIFTDQSVVDRAIN 120
           GK++LTLQRESHVRRLDVLLRVLLIIQQLL +NRHSSKRDIYYMHPSIFTDQSVVDRAIN
Sbjct: 100 GKDVLTLQRESHVRRLDVLLRVLLIIQQLLGENRHSSKRDIYYMHPSIFTDQSVVDRAIN 159

Query: 121 DICILLQCSRHNLNVVSVGNGLVMGWLRFSEAGRRFDCLKKPNSAHFIPVQVEEVTDIAS 180
           DICILLQCSRHNLNVVSVGNGLVMGWLRFSEAGRRFDCLKKPNSAHFIPVQVEEV DI S
Sbjct: 160 DICILLQCSRHNLNVVSVGNGLVMGWLRFSEAGRRFDCLKKPNSAHFIPVQVEEVIDITS 219

Query: 181 AADYILVVEKESVFQRLANDRFCSRNRCIVITGRGYPDVPTRRFLRLLVDVLALPAFCLV 240
           AADYILVVEKESVFQRLANDRFCSRNRCIVITGRGYPDVP+RRFLRLLVDVLALPAFCLV
Sbjct: 220 AADYILVVEKESVFQRLANDRFCSRNRCIVITGRGYPDVPSRRFLRLLVDVLALPAFCLV 279

Query: 241 DCDPYGFDILTTYRFGSMQMAYDAKYLRIPQLQWLGAFASDSEKYNLPEQCLIPLTFKDK 300
           DCDPYGFDILTTYRFGSMQMAYDAKYL+IPQL+WLGAFASD EKYNLPEQCLIPLTFKDK
Sbjct: 280 DCDPYGFDILTTYRFGSMQMAYDAKYLKIPQLRWLGAFASDFEKYNLPEQCLIPLTFKDK 339

Query: 301 RRTEALLQRCYLRREVPHWRLELDSMLERGVKFEIEALSAHNISFLSEEYLPSKIESELT 360
            RTEALLQRCYLRREV HWRLEL+SMLERGVKFEIEALS HNISFLS+EYLPSKIESELT
Sbjct: 340 SRTEALLQRCYLRREVQHWRLELESMLERGVKFEIEALSVHNISFLSDEYLPSKIESELT 399

[Close]

BLAST of PI0005161 vs. NCBI nr
Match: XP_031744443.1 (meiotic recombination protein SPO11-1-like isoform X3 [Cucumis sativus] >XP_031744451.1 meiotic recombination protein SPO11-1-like isoform X3 [Cucumis sativus] >XP_031745627.1 meiotic recombination protein SPO11-1 isoform X3 [Cucumis sativus] >KAE8637132.1 hypothetical protein CSA_021508 [Cucumis sativus])

HSP 1 Score: 689.9 bits (1779), Expect = 1.2e-194
Identity = 339/360 (94.17%), Postives = 353/360 (98.06%), Query Frame = 0


Query: 1   MEGNRGSLDRIEVLRKITELTRSMIVELSNGSSKMIAIDRFKNYCTDSLENCCCRFNLSE 60
           MEGNRGSL+RIEV+RKITELTRSMIVELSNGSS+MIAIDRFK+YC++SLENCCCRFNLSE
Sbjct: 1   MEGNRGSLERIEVMRKITELTRSMIVELSNGSSRMIAIDRFKSYCSNSLENCCCRFNLSE 60

Query: 61  GKEILTLQRESHVRRLDVLLRVLLIIQQLLRDNRHSSKRDIYYMHPSIFTDQSVVDRAIN 120
           GK++LTLQRESHVRRLDVLLRVLLIIQQLL +NRHSSKRDIYYMHPSIFTDQSVVDRAIN
Sbjct: 61  GKDVLTLQRESHVRRLDVLLRVLLIIQQLLGENRHSSKRDIYYMHPSIFTDQSVVDRAIN 120

Query: 121 DICILLQCSRHNLNVVSVGNGLVMGWLRFSEAGRRFDCLKKPNSAHFIPVQVEEVTDIAS 180
           DICILLQCSRHNLNVVSVGNGLVMGWLRFSEAGRRFDCLKKPNSAHFIPVQVEEV DI S
Sbjct: 121 DICILLQCSRHNLNVVSVGNGLVMGWLRFSEAGRRFDCLKKPNSAHFIPVQVEEVIDITS 180

Query: 181 AADYILVVEKESVFQRLANDRFCSRNRCIVITGRGYPDVPTRRFLRLLVDVLALPAFCLV 240
           AADYILVVEKESVFQRLANDRFCSRNRCIVITGRGYPDVP+RRFLRLLVDVLALPAFCLV
Sbjct: 181 AADYILVVEKESVFQRLANDRFCSRNRCIVITGRGYPDVPSRRFLRLLVDVLALPAFCLV 240

Query: 241 DCDPYGFDILTTYRFGSMQMAYDAKYLRIPQLQWLGAFASDSEKYNLPEQCLIPLTFKDK 300
           DCDPYGFDILTTYRFGSMQMAYDAKYL+IPQL+WLGAFASD EKYNLPEQCLIPLTFKDK
Sbjct: 241 DCDPYGFDILTTYRFGSMQMAYDAKYLKIPQLRWLGAFASDFEKYNLPEQCLIPLTFKDK 300

Query: 301 RRTEALLQRCYLRREVPHWRLELDSMLERGVKFEIEALSAHNISFLSEEYLPSKIESELT 360
            RTEALLQRCYLRREV HWRLEL+SMLERGVKFEIEALS HNISFLS+EYLPSKIESELT
Sbjct: 301 SRTEALLQRCYLRREVQHWRLELESMLERGVKFEIEALSVHNISFLSDEYLPSKIESELT 360

[Close]

BLAST of PI0005161 vs. NCBI nr
Match: XP_031744433.1 (meiotic recombination protein SPO11-1-like isoform X3 [Cucumis sativus])

HSP 1 Score: 689.9 bits (1779), Expect = 1.2e-194
Identity = 339/360 (94.17%), Postives = 353/360 (98.06%), Query Frame = 0


Query: 1   MEGNRGSLDRIEVLRKITELTRSMIVELSNGSSKMIAIDRFKNYCTDSLENCCCRFNLSE 60
           MEGNRGSL+RIEV+RKITELTRSMIVELSNGSS+MIAIDRFK+YC++SLENCCCRFNLSE
Sbjct: 12  MEGNRGSLERIEVMRKITELTRSMIVELSNGSSRMIAIDRFKSYCSNSLENCCCRFNLSE 71

Query: 61  GKEILTLQRESHVRRLDVLLRVLLIIQQLLRDNRHSSKRDIYYMHPSIFTDQSVVDRAIN 120
           GK++LTLQRESHVRRLDVLLRVLLIIQQLL +NRHSSKRDIYYMHPSIFTDQSVVDRAIN
Sbjct: 72  GKDVLTLQRESHVRRLDVLLRVLLIIQQLLGENRHSSKRDIYYMHPSIFTDQSVVDRAIN 131

Query: 121 DICILLQCSRHNLNVVSVGNGLVMGWLRFSEAGRRFDCLKKPNSAHFIPVQVEEVTDIAS 180
           DICILLQCSRHNLNVVSVGNGLVMGWLRFSEAGRRFDCLKKPNSAHFIPVQVEEV DI S
Sbjct: 132 DICILLQCSRHNLNVVSVGNGLVMGWLRFSEAGRRFDCLKKPNSAHFIPVQVEEVIDITS 191

Query: 181 AADYILVVEKESVFQRLANDRFCSRNRCIVITGRGYPDVPTRRFLRLLVDVLALPAFCLV 240
           AADYILVVEKESVFQRLANDRFCSRNRCIVITGRGYPDVP+RRFLRLLVDVLALPAFCLV
Sbjct: 192 AADYILVVEKESVFQRLANDRFCSRNRCIVITGRGYPDVPSRRFLRLLVDVLALPAFCLV 251

Query: 241 DCDPYGFDILTTYRFGSMQMAYDAKYLRIPQLQWLGAFASDSEKYNLPEQCLIPLTFKDK 300
           DCDPYGFDILTTYRFGSMQMAYDAKYL+IPQL+WLGAFASD EKYNLPEQCLIPLTFKDK
Sbjct: 252 DCDPYGFDILTTYRFGSMQMAYDAKYLKIPQLRWLGAFASDFEKYNLPEQCLIPLTFKDK 311

Query: 301 RRTEALLQRCYLRREVPHWRLELDSMLERGVKFEIEALSAHNISFLSEEYLPSKIESELT 360
            RTEALLQRCYLRREV HWRLEL+SMLERGVKFEIEALS HNISFLS+EYLPSKIESELT
Sbjct: 312 SRTEALLQRCYLRREVQHWRLELESMLERGVKFEIEALSVHNISFLSDEYLPSKIESELT 371

[Close]

BLAST of PI0005161 vs. NCBI nr
Match: XP_008462990.1 (PREDICTED: meiotic recombination protein SPO11-1 isoform X2 [Cucumis melo])

HSP 1 Score: 685.6 bits (1768), Expect = 2.3e-193
Identity = 343/364 (94.23%), Postives = 355/364 (97.53%), Query Frame = 0


Query: 1   MEGNRGSLDRIEVLRKITELTRSMIVELSNGSSKMIAIDRFKNYCTDSLENCCCRFNLSE 60
           MEGNRGSL+RIEVLRKITELTRSMIVELSNGSSKMIAIDRFK+YCT+SLEN    FNLSE
Sbjct: 1   MEGNRGSLERIEVLRKITELTRSMIVELSNGSSKMIAIDRFKSYCTNSLENW---FNLSE 60

Query: 61  GKEILTLQRESHVRRLDVLLRVLLIIQQLLRDNRHSSKRDIYYMHPSIFTDQSVVDRAIN 120
           GK++LTLQRESHVRRLDVLLRVLLIIQQLLR+NRHSSKRD+YYMHPSIFTDQSVVDRAIN
Sbjct: 61  GKDVLTLQRESHVRRLDVLLRVLLIIQQLLRENRHSSKRDMYYMHPSIFTDQSVVDRAIN 120

Query: 121 DICILLQCSRHNLNVVSVGNGLVMGWLRFSEAGRRFDCLKKPNSAHFIPVQVEEVTDIAS 180
           DICILLQCSRHNLNVVSVGNGLVMGWLRFSEAGRRFDCLKKPNSA FIPVQVEEVTDIA+
Sbjct: 121 DICILLQCSRHNLNVVSVGNGLVMGWLRFSEAGRRFDCLKKPNSAQFIPVQVEEVTDIAT 180

Query: 181 AADYILVVEKESVFQRLANDRFCSRNRCIVITGRGYPDVPTRRFLRLLVDVLALPAFCLV 240
           AADYILVVEKESVFQRLANDRFCSRNRCIVITGRGYPDVPTRRFLRLLVDVLALPAFCLV
Sbjct: 181 AADYILVVEKESVFQRLANDRFCSRNRCIVITGRGYPDVPTRRFLRLLVDVLALPAFCLV 240

Query: 241 DCDPYGFDILTTYRFGSMQMAYDAKYLRIPQLQWLGAFASDSEKYNLPEQCLIPLTFKDK 300
           DCDPYGFDILTTYRFGSMQMAYDAK LRIPQLQW+GAFASDSEKY+LPEQCLIPLT KDK
Sbjct: 241 DCDPYGFDILTTYRFGSMQMAYDAKNLRIPQLQWIGAFASDSEKYDLPEQCLIPLTSKDK 300

Query: 301 RRTEALLQRCYLRREVPHWRLELDSMLERGVKFEIEALSAHNISFLSEEYLPSKIESELT 360
           RRTEALLQRCYLRREVPHWRLELDSML+RGVKFEIEALS HNISFLS+ YLPSKIESELT
Sbjct: 301 RRTEALLQRCYLRREVPHWRLELDSMLQRGVKFEIEALSVHNISFLSDHYLPSKIESELT 360

Query: 361 TGKL 365
           TGKL
Sbjct: 361 TGKL 361

[Close]

BLAST of PI0005161 vs. TAIR 10
Match: AT3G13170.1 (Spo11/DNA topoisomerase VI, subunit A protein )

HSP 1 Score: 498.4 bits (1282), Expect = 4.8e-141
Identity = 237/361 (65.65%), Postives = 297/361 (82.27%), Query Frame = 0


Query: 1   MEGNRGSLDRIEVLRKITELTRSMIVELSNGSSKMIAIDRFKNYCTDSLENCCCRFNLSE 60
           MEG     +   +L++I + T+S++V+L+ G S  I+I++F+NYC +   +C C  +  +
Sbjct: 1   MEGKFAISESTNLLQRIKDFTQSVVVDLAEGRSPKISINQFRNYCMNPEADCLCSSDKPK 60

Query: 61  GKEILTLQRESHVRRLDVLLRVLLIIQQLLRDNRHSSKRDIYYMHPSIFTDQSVVDRAIN 120
           G+EI TL++E    R+D+LLRVLLI+QQLL++NRH+SKRDIYYMHPS F  QS+VDRAI 
Sbjct: 61  GQEIFTLKKEPQTYRIDMLLRVLLIVQQLLQENRHASKRDIYYMHPSAFKAQSIVDRAIG 120

Query: 121 DICILLQCSRHNLNVVSVGNGLVMGWLRFSEAGRRFDCLKKPNSAHFIPVQVEEVTDIAS 180
           DICIL QCSR+NLNVVSVGNGLVMGWL+F EAGR+FDCL   N+A+ +PV VEEV DI S
Sbjct: 121 DICILFQCSRYNLNVVSVGNGLVMGWLKFREAGRKFDCLNSLNTAYPVPVLVEEVEDIVS 180

Query: 181 AADYILVVEKESVFQRLANDRFCSRNRCIVITGRGYPDVPTRRFLRLLVDVLALPAFCLV 240
            A+YILVVEKE+VFQRLAND FC  NRCIVITGRGYPDV TRRFLRLL++ L LP  CLV
Sbjct: 181 LAEYILVVEKETVFQRLANDMFCKTNRCIVITGRGYPDVSTRRFLRLLMEKLHLPVHCLV 240

Query: 241 DCDPYGFDILTTYRFGSMQMAYDAKYLRIPQLQWLGAFASDSEKYNLPEQCLIPLTFKDK 300
           DCDPYGF+IL TYRFGSMQMAYD + LR P ++WLGAF SDSE Y++P+QCL+PLT +DK
Sbjct: 241 DCDPYGFEILATYRFGSMQMAYDIESLRAPDMKWLGAFPSDSEVYSVPKQCLLPLTEEDK 300

Query: 301 RRTEALLQRCYLRREVPHWRLELDSMLERGVKFEIEALSAHNISFLSEEYLPSKIESELT 360
           +RTEA+L RCYL+RE+P WRLEL++ML+RGVKFEIEALS H++SFLSE Y+PSKI  E++
Sbjct: 301 KRTEAMLLRCYLKREMPQWRLELETMLKRGVKFEIEALSVHSLSFLSEVYIPSKIRREVS 360

Query: 361 T 362
           +
Sbjct: 361 S 361

[Close]

BLAST of PI0005161 vs. TAIR 10
Match: AT5G02820.1 (Spo11/DNA topoisomerase VI, subunit A protein )

HSP 1 Score: 233.8 bits (595), Expect = 2.2e-61
Identity = 118/288 (40.97%), Postives = 178/288 (61.81%), Query Frame = 0


Query: 71  SHVRRLDVLLRVLLIIQQLLRDNRHSSKRDIYYMHPSIFTDQSVVDRAINDICILLQCSR 130
           S VR+  +  R+L +I QL   N H +KRD++Y    +F DQ+  D  ++D+  +L C+R
Sbjct: 138 SSVRKTTITTRILALIHQLCLRNIHVTKRDLFYTDVKLFQDQTQSDAVLDDVSCMLGCTR 197

Query: 131 HNLNVVSVGNGLVMGWLRFSEAGRRFDCLKKPNSAHFIPVQVEEVTDIASAADYILVVEK 190
            +LNV++   G+V+G L FS+ G   DC K       IP  ++ V D+ S A +IL+VEK
Sbjct: 198 SSLNVIAAEKGVVVGRLIFSDNGDMIDCTKMGMGGKAIPPNIDRVGDMQSDAMFILLVEK 257

Query: 191 ESVFQRLANDRFCSRNRCIVITGRGYPDVPTRRFLRLLVDVLALPAFCLVDCDPYGFDIL 250
           ++ + RLA DRF +R  CI++T +G PDV TR FLR +   L LP   LVD DPYG  IL
Sbjct: 258 DAAYMRLAEDRFYNRFPCIIVTAKGQPDVATRLFLRKMKMELKLPVLALVDSDPYGLKIL 317

Query: 251 TTYRFGSMQMAYDAKYLRIPQLQWLGAFASDSEKYNLPEQCLIPLTFKDKRRTEALLQRC 310
           + Y  GS  M+YD+  L  P ++WLG   SD +KY +PEQC +P+T +D +  + +L+  
Sbjct: 318 SVYGCGSKNMSYDSANLTTPDIKWLGIRPSDLDKYKIPEQCRLPMTEQDIKTGKDMLEED 377

Query: 311 YLRREVPHWRLELDSMLERGVKFEIEALSAHNISFLSEEYLPSKIESE 359
           ++++  P W  EL+ M++   K EI+ALS+    +LSE YLP K++ +
Sbjct: 378 FVKKN-PGWVEELNLMVKTKQKAEIQALSSFGFQYLSEVYLPLKLQQQ 424

[Close]

BLAST of PI0005161 vs. TAIR 10
Match: AT1G63990.1 (sporulation 11-2 )

HSP 1 Score: 148.3 bits (373), Expect = 1.2e-35
Identity = 96/283 (33.92%), Postives = 157/283 (55.48%), Query Frame = 0


Query: 81  RVLLIIQQLLRDNRHSSKRDIYYM----HPSIFTDQSVVDRAINDICILLQCSRHNLNVV 140
           +V+ I  Q+L   +  ++R+++Y      P  F+ Q  V+R++ D+  LL+CSR++L ++
Sbjct: 102 KVMEICFQILLQEKRVTQRELFYKLLCDSPDYFSSQIEVNRSVQDVVALLRCSRYSLGIM 161

Query: 141 SVGNGLVMGWLRFSEAGRR-FDCLKKPNSAHFI--PVQVEEVTDIASAADYILVVEKESV 200
           +   GLV G L   E G+   DC    +S   I   + + + T + + A YI++VEK ++
Sbjct: 162 ASSRGLVAGRLFLQEPGKEAVDCSACGSSGFAITGDLNLLDNTIMRTDARYIIIVEKHAI 221

Query: 201 FQRLANDRFCSRNRCIVITGRGYPDVPTRRFL-RLLVDVLALPAFCLVDCDPYGFDILTT 260
           F RL  DR  +   C+ IT +GYPD+ TR FL R+      LP   LVD +P G  IL T
Sbjct: 222 FHRLVEDRVFNHIPCVFITAKGYPDIATRFFLHRMSTTFPDLPILVLVDWNPAGLAILCT 281

Query: 261 YRFGSMQMAYDAKYLRIPQLQWLGAFASDSEKYNLPEQCLIPLTFKDKRRTEALLQRCYL 320
           ++FGS+ M  +A Y     ++W+G    D     +PE+ L+PL  KD +  ++LL    L
Sbjct: 282 FKFGSIGMGLEA-YRYACNVKWIGLRGDDLNL--IPEESLVPLKPKDSQIAKSLLSSKIL 341

Query: 321 RREVPHWRLELDSMLERGVKFEIEALSAHNISFLSEEYLPSKI 356
           +    ++  EL  M++ G + EIEAL  H  ++L  +Y+ +KI
Sbjct: 342 QE---NYIEELSLMVQTGKRAEIEALYCHGYNYLG-KYIATKI 377

The following BLAST results are available for this feature:

BLAST of PI0005161 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9M4A2	6.7e-140	65.65	Meiotic recombination protein SPO11-1 OS=Arabidopsis thaliana OX=3702 GN=SPO11-1...	[more]
Q5ZPV8	2.7e-125	58.89	Meiotic recombination protein SPO11-1 OS=Oryza sativa subsp. indica OX=39946 GN=...	[more]
Q7Y021	2.7e-125	58.89	Meiotic recombination protein SPO11-1 OS=Oryza sativa subsp. japonica OX=39947 G...	[more]
Q9LZ03	3.1e-60	40.97	DNA topoisomerase 6 subunit A OS=Arabidopsis thaliana OX=3702 GN=TOP6A PE=1 SV=1	[more]
Q9Y5K1	7.6e-59	34.65	Meiotic recombination protein SPO11 OS=Homo sapiens OX=9606 GN=SPO11 PE=1 SV=1	[more]

BLAST of PI0005161 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3CJR6	5.1e-199	95.33	meiotic recombination protein SPO11-1 isoform X1 OS=Cucumis melo OX=3656 GN=LOC1...	[more]
A0A1S3CI64	1.1e-193	94.23	meiotic recombination protein SPO11-1 isoform X2 OS=Cucumis melo OX=3656 GN=LOC1...	[more]
A0A6J1IGM0	5.6e-190	90.91	meiotic recombination protein SPO11-1 OS=Cucurbita maxima OX=3661 GN=LOC11147676...	[more]
A0A6J1F921	5.3e-188	89.86	meiotic recombination protein SPO11-1 OS=Cucurbita moschata OX=3662 GN=LOC111443...	[more]
A0A6J1CDC7	4.2e-185	87.91	meiotic recombination protein SPO11-1 OS=Momordica charantia OX=3673 GN=LOC11101...	[more]

BLAST of PI0005161 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008462989.1	1.0e-198	95.33	PREDICTED: meiotic recombination protein SPO11-1 isoform X1 [Cucumis melo]	[more]
XP_031745985.1	1.2e-194	94.17	meiotic recombination protein SPO11-1-like isoform X3 [Cucumis sativus]	[more]
XP_031744443.1	1.2e-194	94.17	meiotic recombination protein SPO11-1-like isoform X3 [Cucumis sativus] >XP_0317...	[more]
XP_031744433.1	1.2e-194	94.17	meiotic recombination protein SPO11-1-like isoform X3 [Cucumis sativus]	[more]
XP_008462990.1	2.3e-193	94.23	PREDICTED: meiotic recombination protein SPO11-1 isoform X2 [Cucumis melo]	[more]

BLAST of PI0005161 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G13170.1	4.8e-141	65.65	Spo11/DNA topoisomerase VI, subunit A protein	[more]
AT5G02820.1	2.2e-61	40.97	Spo11/DNA topoisomerase VI, subunit A protein	[more]
AT1G63990.1	1.2e-35	33.92	sporulation 11-2	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR013048	Meiotic recombination, Spo11	PRINTS	PR01551	SPO11HOMOLOG	coord: 342..361 score: 30.0 coord: 281..305 score: 36.0 coord: 199..214 score: 27.68 coord: 104..124 score: 40.14 coord: 246..265 score: 40.0
IPR002815	Spo11/DNA topoisomerase VI subunit A	PRINTS	PR01550	TOP6AFAMILY	coord: 112..129 score: 43.06 coord: 130..145 score: 41.41 coord: 209..230 score: 46.88 coord: 241..257 score: 52.57 coord: 98..109 score: 49.48 coord: 185..202 score: 54.51
IPR002815	Spo11/DNA topoisomerase VI subunit A	PANTHER	PTHR10848	MEIOTIC RECOMBINATION PROTEIN SPO11	coord: 10..357
IPR013049	Spo11/DNA topoisomerase VI, subunit A, N-terminal	PFAM	PF04406	TP6A_N	coord: 74..135 e-value: 2.0E-21 score: 75.8
IPR036388	Winged helix-like DNA-binding domain superfamily	GENE3D	1.10.10.10		coord: 3..138 e-value: 1.1E-22 score: 82.4
None	No IPR available	GENE3D	3.40.1360.10		coord: 140..359 e-value: 1.1E-81 score: 275.7
None	No IPR available	PANTHER	PTHR10848:SF3	MEIOTIC RECOMBINATION PROTEIN SPO11-1	coord: 10..357
IPR034136	Topoisomerase 6 subunit A/Spo11, TOPRIM domain	CDD	cd00223	TOPRIM_TopoIIB_SPO	coord: 183..345 e-value: 1.04925E-66 score: 205.952
IPR036078	Spo11/DNA topoisomerase VI subunit A superfamily	SUPERFAMILY	56726	DNA topoisomerase IV, alpha subunit	coord: 73..357

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
PI0005161.1	PI0005161.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0051026	chiasma assembly
biological_process	GO:0000724	double-strand break repair via homologous recombination
biological_process	GO:0042138	meiotic DNA double-strand break formation
biological_process	GO:0006259	DNA metabolic process
cellular_component	GO:0005694	chromosome
cellular_component	GO:0005634	nucleus
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0003677	DNA binding
molecular_function	GO:0003918	DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity
molecular_function	GO:0016787	hydrolase activity
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0003824	catalytic activity