PI0003842 (gene) Melon (PI 482460) v1

Overview


Name	PI0003842
Type	gene
Organism	Cucumis metuliferus (Melon (PI 482460) v1)
Description	RING-type domain-containing protein
Location	chr08: 7074040 .. 7074765 (-)
RNA-Seq Expression	PI0003842
Synteny	PI0003842

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGGTGGAATATCAAATCTCTCGATACTCAACTATGGCAGTGGAAGTAGATACAGATCTTCTGATGTTGACAAAAATCCCGAAGCATCCATCACCAATCGAACTAATTGGCTTGCTCTTCACCCTACGTCGCCGTTTCAACCACTTTCAGGTGTTAGAGAATGGTGAACGACAATTAATAGAGGAACGAGAAATATCAAAAGTTCCTCTATGTGACCACCAATTCCCCGTTGAAATCTTTAATGGAGACAACAATTCTCTATTCCAATACCTTTGTTGTAGCTTCGAAAACTACGAATCTAAGATAGATGCAGAGGTAGTCGCAAGTGTCCTAATCGAGCAGTGGATTGATATAAGGGACCAAGTGAATTCGAGAAGCTGCGATAAAATATTTTCAATGGCAGTAAATTTAACAATTACAGATGTTGTGGTTCGATATGTTATTGGCGATGATGGCAACACATTTGGTGGAGAAGATTTTAGTGTGGTTCCAGCATGCGACAAAACAGTTAAGAAGATGTTGAAGAGATTGGAAATGAGAGACGAAGATAAAAGAATTTGTGTGATATGTTTGGATGAAGTTAAGAAAAAGAAGAATGGAAGATGTTTGGAAATGCCTTGTTTACATGTATTTCATGGGGAATGCATTGAAAATTGGTTGAAAAATAGTCATTGTTGTCCAATTTGTAGATTTGAAATGCCAGTGAAATTTTTGGAAACGTGA

mRNA sequence

ATGGAGGTGGAATATCAAATCTCTCGATACTCAACTATGGCAGTGGAAGTAGATACAGATCTTCTGATGTTGACAAAAATCCCGAAGCATCCATCACCAATCGAACTAATTGGCTTGCTCTTCACCCTACGTCGCCGTTTCAACCACTTTCAGGTGTTAGAGAATGGTGAACGACAATTAATAGAGGAACGAGAAATATCAAAAGTTCCTCTATGTGACCACCAATTCCCCGTTGAAATCTTTAATGGAGACAACAATTCTCTATTCCAATACCTTTGTTGTAGCTTCGAAAACTACGAATCTAAGATAGATGCAGAGGTAGTCGCAAGTGTCCTAATCGAGCAGTGGATTGATATAAGGGACCAAGTGAATTCGAGAAGCTGCGATAAAATATTTTCAATGGCAGTAAATTTAACAATTACAGATGTTGTGGTTCGATATGTTATTGGCGATGATGGCAACACATTTGGTGGAGAAGATTTTAGTGTGGTTCCAGCATGCGACAAAACAGTTAAGAAGATGTTGAAGAGATTGGAAATGAGAGACGAAGATAAAAGAATTTGTGTGATATGTTTGGATGAAGTTAAGAAAAAGAAGAATGGAAGATGTTTGGAAATGCCTTGTTTACATGTATTTCATGGGGAATGCATTGAAAATTGGTTGAAAAATAGTCATTGTTGTCCAATTTGTAGATTTGAAATGCCAGTGAAATTTTTGGAAACGTGA

Coding sequence (CDS)

ATGGAGGTGGAATATCAAATCTCTCGATACTCAACTATGGCAGTGGAAGTAGATACAGATCTTCTGATGTTGACAAAAATCCCGAAGCATCCATCACCAATCGAACTAATTGGCTTGCTCTTCACCCTACGTCGCCGTTTCAACCACTTTCAGGTGTTAGAGAATGGTGAACGACAATTAATAGAGGAACGAGAAATATCAAAAGTTCCTCTATGTGACCACCAATTCCCCGTTGAAATCTTTAATGGAGACAACAATTCTCTATTCCAATACCTTTGTTGTAGCTTCGAAAACTACGAATCTAAGATAGATGCAGAGGTAGTCGCAAGTGTCCTAATCGAGCAGTGGATTGATATAAGGGACCAAGTGAATTCGAGAAGCTGCGATAAAATATTTTCAATGGCAGTAAATTTAACAATTACAGATGTTGTGGTTCGATATGTTATTGGCGATGATGGCAACACATTTGGTGGAGAAGATTTTAGTGTGGTTCCAGCATGCGACAAAACAGTTAAGAAGATGTTGAAGAGATTGGAAATGAGAGACGAAGATAAAAGAATTTGTGTGATATGTTTGGATGAAGTTAAGAAAAAGAAGAATGGAAGATGTTTGGAAATGCCTTGTTTACATGTATTTCATGGGGAATGCATTGAAAATTGGTTGAAAAATAGTCATTGTTGTCCAATTTGTAGATTTGAAATGCCAGTGAAATTTTTGGAAACGTGA

Protein sequence

MEVEYQISRYSTMAVEVDTDLLMLTKIPKHPSPIELIGLLFTLRRRFNHFQVLENGERQLIEEREISKVPLCDHQFPVEIFNGDNNSLFQYLCCSFENYESKIDAEVVASVLIEQWIDIRDQVNSRSCDKIFSMAVNLTITDVVVRYVIGDDGNTFGGEDFSVVPACDKTVKKMLKRLEMRDEDKRICVICLDEVKKKKNGRCLEMPCLHVFHGECIENWLKNSHCCPICRFEMPVKFLET

Homology

[Close]

BLAST of PI0003842 vs. ExPASy Swiss-Prot
Match: Q9LQX2 (E3 ubiquitin-protein ligase MPSR1 OS=Arabidopsis thaliana OX=3702 GN=MPSR1 PE=1 SV=1)

HSP 1 Score: 65.9 bits (159), Expect = 7.4e-10
Identity = 28/49 (57.14%), Postives = 34/49 (69.39%), Query Frame = 0


Query: 188 CVICLDEVKKKKNGRCLEMPCLHVFHGECIENWLKNSHCCPICRFEMPV 237
           CVICL+E K ++  +  EMPC H FHG CIE WL     CP+CR+EMPV
Sbjct: 113 CVICLEEWKSEETVK--EMPCKHRFHGGCIEKWLGFHGSCPVCRYEMPV 159

[Close]

BLAST of PI0003842 vs. ExPASy Swiss-Prot
Match: Q9SNB6 (E3 ubiquitin-protein ligase RDUF1 OS=Arabidopsis thaliana OX=3702 GN=RDUF1 PE=1 SV=1)

HSP 1 Score: 64.3 bits (155), Expect = 2.1e-09
Identity = 29/74 (39.19%), Postives = 43/74 (58.11%), Query Frame = 0


Query: 165 PACDKTVKKMLKRLEMRD---EDKRICVICLDEVKKKKNGRCLEMPCLHVFHGECIENWL 224
           P   K+  + L R+E+ D   + +  C +C +  +    GR  EMPC H+FHG+CI  WL
Sbjct: 189 PPASKSAIESLPRVEISDCHTKAEANCAVCTEVFEAGIEGR--EMPCKHIFHGDCIVPWL 248

Query: 225 KNSHCCPICRFEMP 236
              + CP+CRFE+P
Sbjct: 249 SIRNSCPVCRFELP 260

[Close]

BLAST of PI0003842 vs. ExPASy Swiss-Prot
Match: Q9P0P0 (E3 ubiquitin-protein ligase RNF181 OS=Homo sapiens OX=9606 GN=RNF181 PE=1 SV=1)

HSP 1 Score: 63.9 bits (154), Expect = 2.8e-09
Identity = 29/77 (37.66%), Postives = 45/77 (58.44%), Query Frame = 0


Query: 160 DFSVVPACDKTVKKMLKRLEMRDEDKRI-CVICLDEVKKKKNGRCLEMPCLHVFHGECIE 219
           D  + P   KTV + L R  +R     + C +CL E ++++    +EMPC H+FH  CI 
Sbjct: 47  DHHLPPPAAKTVVENLPRTVIRGSQAELKCPVCLLEFEEEET--AIEMPCHHLFHSSCIL 106

Query: 220 NWLKNSHCCPICRFEMP 236
            WL  ++ CP+CR+E+P
Sbjct: 107 PWLSKTNSCPLCRYELP 121

[Close]

BLAST of PI0003842 vs. ExPASy Swiss-Prot
Match: Q3T0W3 (E3 ubiquitin-protein ligase RNF181 OS=Bos taurus OX=9913 GN=RNF181 PE=2 SV=1)

HSP 1 Score: 60.8 bits (146), Expect = 2.4e-08
Identity = 28/77 (36.36%), Postives = 43/77 (55.84%), Query Frame = 0


Query: 160 DFSVVPACDKTVKKMLKRLEMRDEDKRI-CVICLDEVKKKKNGRCLEMPCLHVFHGECIE 219
           D  + P   KT  + L R  +R     + C +CL E ++++    +EMPC H+FH  CI 
Sbjct: 47  DHHLPPPAAKTAVENLPRTVIRGSQAELKCPVCLLEFEEEET--AIEMPCHHLFHSNCIL 106

Query: 220 NWLKNSHCCPICRFEMP 236
            WL  ++ CP+CR E+P
Sbjct: 107 PWLSKTNSCPLCRHELP 121

[Close]

BLAST of PI0003842 vs. ExPASy Swiss-Prot
Match: Q08109 (ERAD-associated E3 ubiquitin-protein ligase HRD1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HRD1 PE=1 SV=1)

HSP 1 Score: 59.7 bits (143), Expect = 5.3e-08
Identity = 26/72 (36.11%), Postives = 40/72 (55.56%), Query Frame = 0


Query: 168 DKTVKKMLKRLEMRDEDKRICVICLDEV--------KKKKNGRCLEMPCLHVFHGECIEN 227
           D  V   +++L+    D  IC+IC+DE+         K KN +   +PC H+ H  C++N
Sbjct: 329 DTLVTVTVEQLQNSANDDNICIICMDELIHSPNQQTWKNKNKKPKRLPCGHILHLSCLKN 388

Query: 228 WLKNSHCCPICR 232
           W++ S  CPICR
Sbjct: 389 WMERSQTCPICR 400

[Close]

BLAST of PI0003842 vs. ExPASy TrEMBL
Match: A0A0A0L236 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G419590 PE=4 SV=1)

HSP 1 Score: 418.3 bits (1074), Expect = 2.2e-113
Identity = 199/235 (84.68%), Postives = 219/235 (93.19%), Query Frame = 0


Query: 3   VEYQISRYSTMAVEVDTDLLMLTKIPKHPSPIELIGLLFTLRRRFNHFQVLENGERQLIE 62
           +EYQISRYST AVEVD D+   TKIPKHPSPIELIGLLFTLRRR N FQVLENGERQL+E
Sbjct: 1   MEYQISRYSTTAVEVDIDVPKWTKIPKHPSPIELIGLLFTLRRRCNRFQVLENGERQLVE 60

Query: 63  EREISKVPLCDHQFPVEIFNGDNNSLFQYLCCSFENYESKIDAEVVASVLIEQWIDIRDQ 122
           E+EISKVPLCDHQFPVEIFNGD++SLFQYLCCSFENYESKIDAEVVASVLIEQWI+IR+Q
Sbjct: 61  EQEISKVPLCDHQFPVEIFNGDSSSLFQYLCCSFENYESKIDAEVVASVLIEQWIEIRNQ 120

Query: 123 VNSRSCDKIFSMAVNLTITDVVVRYVIGDDGNTFGGEDFSVVPACDKTVKKMLKRLEMRD 182
           VNSRS DK+FSM VNLTITDVV++YV+G+DG+TFGGED  VVPACDK +KKMLKRLEMR+
Sbjct: 121 VNSRSRDKVFSMTVNLTITDVVLQYVVGEDGSTFGGEDHRVVPACDKALKKMLKRLEMRE 180

Query: 183 EDKRICVICLDEVKKKKNGRC-LEMPCLHVFHGECIENWLKNSHCCPICRFEMPV 237
           ED+R+CVICLDEV KKKNGR  LEMPCLHVFHG+CIENW KNSHCCPICRFEMP+
Sbjct: 181 EDERVCVICLDEVGKKKNGRIGLEMPCLHVFHGKCIENWFKNSHCCPICRFEMPI 235

[Close]

BLAST of PI0003842 vs. ExPASy TrEMBL
Match: A0A5A7SNF1 (RING finger protein 141-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold447G00770 PE=4 SV=1)

HSP 1 Score: 416.8 bits (1070), Expect = 6.3e-113
Identity = 195/235 (82.98%), Postives = 217/235 (92.34%), Query Frame = 0


Query: 3   VEYQISRYSTMAVEVDTDLLMLTKIPKHPSPIELIGLLFTLRRRFNHFQVLENGERQLIE 62
           +EYQIS+YST AVE+D DL M  +I KHPSPIELIGLLFTLRRR N FQVLENGERQL+E
Sbjct: 1   MEYQISQYSTTAVEIDIDLPMSRRIRKHPSPIELIGLLFTLRRRCNRFQVLENGERQLVE 60

Query: 63  EREISKVPLCDHQFPVEIFNGDNNSLFQYLCCSFENYESKIDAEVVASVLIEQWIDIRDQ 122
           E+E SKVPLCDHQFPVEIFNGD+NSLFQYLCCSFENYE KIDAEVVA+ LIEQWI IRDQ
Sbjct: 61  EQEASKVPLCDHQFPVEIFNGDDNSLFQYLCCSFENYEPKIDAEVVATALIEQWIGIRDQ 120

Query: 123 VNSRSCDKIFSMAVNLTITDVVVRYVIGDDGNTFGGEDFSVVPACDKTVKKMLKRLEMRD 182
           +NSRSC K+FSM VNLTITDVVV+YVIG+DG++FGGEDF  VPACDK +KKMLKRLE+RD
Sbjct: 121 LNSRSCHKVFSMTVNLTITDVVVQYVIGEDGSSFGGEDFRAVPACDKALKKMLKRLEVRD 180

Query: 183 EDKRICVICLDEVKKKKNGRC-LEMPCLHVFHGECIENWLKNSHCCPICRFEMPV 237
           ED+RIC+ICLDEV+KKKNGRC LEMPCLHVFHG+CIENWLKNSHCCPICRF+MP+
Sbjct: 181 EDERICIICLDEVRKKKNGRCGLEMPCLHVFHGKCIENWLKNSHCCPICRFQMPI 235

[Close]

BLAST of PI0003842 vs. ExPASy TrEMBL
Match: A0A1S4E0Q9 (RING finger protein 141-like OS=Cucumis melo OX=3656 GN=LOC103495859 PE=4 SV=1)

HSP 1 Score: 416.8 bits (1070), Expect = 6.3e-113
Identity = 195/235 (82.98%), Postives = 217/235 (92.34%), Query Frame = 0


Query: 3   VEYQISRYSTMAVEVDTDLLMLTKIPKHPSPIELIGLLFTLRRRFNHFQVLENGERQLIE 62
           +EYQIS+YST AVE+D DL M  +I KHPSPIELIGLLFTLRRR N FQVLENGERQL+E
Sbjct: 1   MEYQISQYSTTAVEIDIDLPMSRRIRKHPSPIELIGLLFTLRRRCNRFQVLENGERQLVE 60

Query: 63  EREISKVPLCDHQFPVEIFNGDNNSLFQYLCCSFENYESKIDAEVVASVLIEQWIDIRDQ 122
           E+E SKVPLCDHQFPVEIFNGD+NSLFQYLCCSFENYE KIDAEVVA+ LIEQWI IRDQ
Sbjct: 61  EQEASKVPLCDHQFPVEIFNGDDNSLFQYLCCSFENYEPKIDAEVVATALIEQWIGIRDQ 120

Query: 123 VNSRSCDKIFSMAVNLTITDVVVRYVIGDDGNTFGGEDFSVVPACDKTVKKMLKRLEMRD 182
           +NSRSC K+FSM VNLTITDVVV+YVIG+DG++FGGEDF  VPACDK +KKMLKRLE+RD
Sbjct: 121 LNSRSCHKVFSMTVNLTITDVVVQYVIGEDGSSFGGEDFRAVPACDKALKKMLKRLEVRD 180

Query: 183 EDKRICVICLDEVKKKKNGRC-LEMPCLHVFHGECIENWLKNSHCCPICRFEMPV 237
           ED+RIC+ICLDEV+KKKNGRC LEMPCLHVFHG+CIENWLKNSHCCPICRF+MP+
Sbjct: 181 EDERICIICLDEVRKKKNGRCGLEMPCLHVFHGKCIENWLKNSHCCPICRFQMPI 235

[Close]

BLAST of PI0003842 vs. ExPASy TrEMBL
Match: A0A6J1GK53 (ERAD-associated E3 ubiquitin-protein ligase HRD1-like OS=Cucurbita moschata OX=3662 GN=LOC111455016 PE=4 SV=1)

HSP 1 Score: 295.8 bits (756), Expect = 1.6e-76
Identity = 147/238 (61.76%), Postives = 183/238 (76.89%), Query Frame = 0


Query: 3   VEYQISRYSTMAVEVDTDLLMLTKIPKHPSPIELIGLLFTLRRRFNHFQVLENGERQLIE 62
           +E+QISRY  + VEV TDL   ++IPKHPS IE IGL+FTLRRR +HF+V ENGER+LI+
Sbjct: 1   MEHQISRYENLVVEVLTDLPTPSRIPKHPSRIEHIGLIFTLRRRRSHFEVSENGERRLIQ 60

Query: 63  EREISKVPLCDHQFPVEIFNGDNNSLFQYLCCSFENYESKIDAEVVASVLIEQWIDIRDQ 122
           +RE  K+PLCD+QF  EIFN D++SLF  L CSF+N++ +IDAE VA  LIE+WI+IRD+
Sbjct: 61  QRETLKIPLCDNQFRAEIFNRDDDSLFHSLSCSFQNFKPEIDAEPVADALIERWIEIRDE 120

Query: 123 VNSRSCDKIFSMAVNLTITDVVVRYVIGDDGNTFGGEDFSVVPACDKTVKKMLKRLEMRD 182
            NSRS D++F M VNLT+  +V RY IG      GG+   +VPAC   V KML+RL+  D
Sbjct: 121 ANSRSSDRVFPMTVNLTMWHIVARYEIGVGS---GGDGCGMVPACGGAVAKMLRRLKRED 180

Query: 183 EDKRICVICLDEV--KKKKNGRC-LEMPCLHVFHGECIENWLKNSHCCPICRFEMPVK 238
           E   ICVICLDE+  KKKKNGR  L+MPCLHVFHG CIE+WLKNSH CPICRFE+P++
Sbjct: 181 EVDGICVICLDELKNKKKKNGRFGLQMPCLHVFHGNCIEDWLKNSHYCPICRFEVPIQ 235

[Close]

BLAST of PI0003842 vs. ExPASy TrEMBL
Match: A0A6J1I8N9 (ERAD-associated E3 ubiquitin-protein ligase HRD1-like OS=Cucurbita maxima OX=3661 GN=LOC111471014 PE=4 SV=1)

HSP 1 Score: 289.3 bits (739), Expect = 1.5e-74
Identity = 145/238 (60.92%), Postives = 181/238 (76.05%), Query Frame = 0


Query: 3   VEYQISRYSTMAVEVDTDLLMLTKIPKHPSPIELIGLLFTLRRRFNHFQVLENGERQLIE 62
           +E+QISRY  + VEV TDL   ++IPKHPS IE IGL+FTLR R +HF+V E+GER+LI+
Sbjct: 1   MEHQISRYENLVVEVLTDLPTPSRIPKHPSRIEHIGLIFTLRHRRSHFEVSESGERRLIQ 60

Query: 63  EREISKVPLCDHQFPVEIFNGDNNSLFQYLCCSFENYESKIDAEVVASVLIEQWIDIRDQ 122
           +RE  K+PLCD+QF  EIFN D++SLF  L  SF+N++ +IDAE VA  LIE+WI+IRD+
Sbjct: 61  QRETLKIPLCDNQFRAEIFNRDDDSLFHNLSSSFQNFKPEIDAEPVADALIERWIEIRDE 120

Query: 123 VNSRSCDKIFSMAVNLTITDVVVRYVIGDDGNTFGGEDFSVVPACDKTVKKMLKRLEMRD 182
            NSRS D++F M VNLT+  +V RY IG      GG+   +VPAC   V KML+RLE  D
Sbjct: 121 ANSRSSDRVFPMTVNLTMWHIVARYEIGVGS---GGDGCGMVPACGGAVGKMLRRLERED 180

Query: 183 EDKRICVICLDEV--KKKKNGRC-LEMPCLHVFHGECIENWLKNSHCCPICRFEMPVK 238
           E   ICVICLDE+  KKKKNGR  L+MPCLHVFHG CIE+WLKNSH CPICRFE+P++
Sbjct: 181 EVDGICVICLDELKKKKKKNGRFGLQMPCLHVFHGNCIEDWLKNSHYCPICRFEVPIQ 235

[Close]

BLAST of PI0003842 vs. NCBI nr
Match: XP_011654386.1 (RING finger protein 141 [Cucumis sativus] >KGN54667.1 hypothetical protein Csa_012982 [Cucumis sativus])

HSP 1 Score: 418.3 bits (1074), Expect = 4.5e-113
Identity = 199/235 (84.68%), Postives = 219/235 (93.19%), Query Frame = 0


Query: 3   VEYQISRYSTMAVEVDTDLLMLTKIPKHPSPIELIGLLFTLRRRFNHFQVLENGERQLIE 62
           +EYQISRYST AVEVD D+   TKIPKHPSPIELIGLLFTLRRR N FQVLENGERQL+E
Sbjct: 1   MEYQISRYSTTAVEVDIDVPKWTKIPKHPSPIELIGLLFTLRRRCNRFQVLENGERQLVE 60

Query: 63  EREISKVPLCDHQFPVEIFNGDNNSLFQYLCCSFENYESKIDAEVVASVLIEQWIDIRDQ 122
           E+EISKVPLCDHQFPVEIFNGD++SLFQYLCCSFENYESKIDAEVVASVLIEQWI+IR+Q
Sbjct: 61  EQEISKVPLCDHQFPVEIFNGDSSSLFQYLCCSFENYESKIDAEVVASVLIEQWIEIRNQ 120

Query: 123 VNSRSCDKIFSMAVNLTITDVVVRYVIGDDGNTFGGEDFSVVPACDKTVKKMLKRLEMRD 182
           VNSRS DK+FSM VNLTITDVV++YV+G+DG+TFGGED  VVPACDK +KKMLKRLEMR+
Sbjct: 121 VNSRSRDKVFSMTVNLTITDVVLQYVVGEDGSTFGGEDHRVVPACDKALKKMLKRLEMRE 180

Query: 183 EDKRICVICLDEVKKKKNGRC-LEMPCLHVFHGECIENWLKNSHCCPICRFEMPV 237
           ED+R+CVICLDEV KKKNGR  LEMPCLHVFHG+CIENW KNSHCCPICRFEMP+
Sbjct: 181 EDERVCVICLDEVGKKKNGRIGLEMPCLHVFHGKCIENWFKNSHCCPICRFEMPI 235

[Close]

BLAST of PI0003842 vs. NCBI nr
Match: XP_016901804.1 (PREDICTED: RING finger protein 141-like [Cucumis melo] >KAA0025781.1 RING finger protein 141-like [Cucumis melo var. makuwa] >TYK09674.1 RING finger protein 141-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 416.8 bits (1070), Expect = 1.3e-112
Identity = 195/235 (82.98%), Postives = 217/235 (92.34%), Query Frame = 0


Query: 3   VEYQISRYSTMAVEVDTDLLMLTKIPKHPSPIELIGLLFTLRRRFNHFQVLENGERQLIE 62
           +EYQIS+YST AVE+D DL M  +I KHPSPIELIGLLFTLRRR N FQVLENGERQL+E
Sbjct: 1   MEYQISQYSTTAVEIDIDLPMSRRIRKHPSPIELIGLLFTLRRRCNRFQVLENGERQLVE 60

Query: 63  EREISKVPLCDHQFPVEIFNGDNNSLFQYLCCSFENYESKIDAEVVASVLIEQWIDIRDQ 122
           E+E SKVPLCDHQFPVEIFNGD+NSLFQYLCCSFENYE KIDAEVVA+ LIEQWI IRDQ
Sbjct: 61  EQEASKVPLCDHQFPVEIFNGDDNSLFQYLCCSFENYEPKIDAEVVATALIEQWIGIRDQ 120

Query: 123 VNSRSCDKIFSMAVNLTITDVVVRYVIGDDGNTFGGEDFSVVPACDKTVKKMLKRLEMRD 182
           +NSRSC K+FSM VNLTITDVVV+YVIG+DG++FGGEDF  VPACDK +KKMLKRLE+RD
Sbjct: 121 LNSRSCHKVFSMTVNLTITDVVVQYVIGEDGSSFGGEDFRAVPACDKALKKMLKRLEVRD 180

Query: 183 EDKRICVICLDEVKKKKNGRC-LEMPCLHVFHGECIENWLKNSHCCPICRFEMPV 237
           ED+RIC+ICLDEV+KKKNGRC LEMPCLHVFHG+CIENWLKNSHCCPICRF+MP+
Sbjct: 181 EDERICIICLDEVRKKKNGRCGLEMPCLHVFHGKCIENWLKNSHCCPICRFQMPI 235

[Close]

BLAST of PI0003842 vs. NCBI nr
Match: KAG6571896.1 (E3 ubiquitin-protein ligase MPSR1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 298.1 bits (762), Expect = 6.7e-77
Identity = 149/238 (62.61%), Postives = 183/238 (76.89%), Query Frame = 0


Query: 3   VEYQISRYSTMAVEVDTDLLMLTKIPKHPSPIELIGLLFTLRRRFNHFQVLENGERQLIE 62
           +E+QISRY  + VEV TDL   ++IPKHPS IE IGL+FTLRRR +HF+V ENGER+LI+
Sbjct: 1   MEHQISRYENLVVEVLTDLPTPSRIPKHPSRIEHIGLIFTLRRRRSHFEVSENGERRLIQ 60

Query: 63  EREISKVPLCDHQFPVEIFNGDNNSLFQYLCCSFENYESKIDAEVVASVLIEQWIDIRDQ 122
           +RE  K+PLCD+QF  EIFN D++SLF  L CSF+N++ +IDAE VA  LIE+WI+IRD+
Sbjct: 61  QRETLKIPLCDNQFRAEIFNRDDDSLFHSLSCSFQNFKPEIDAEPVADALIERWIEIRDE 120

Query: 123 VNSRSCDKIFSMAVNLTITDVVVRYVIGDDGNTFGGEDFSVVPACDKTVKKMLKRLEMRD 182
            NSRS D++F M VNLTI  +V RY IG      GG+   +VPAC   V KML+RLE  D
Sbjct: 121 ANSRSSDRVFPMTVNLTIWHIVARYEIGVGS---GGDGCGMVPACGGAVGKMLRRLERED 180

Query: 183 EDKRICVICLDEV--KKKKNGRC-LEMPCLHVFHGECIENWLKNSHCCPICRFEMPVK 238
           E   ICVICLDE+  KKKKNGR  L+MPCLHVFHG CIE+WLKNSH CPICRFE+P++
Sbjct: 181 EVDGICVICLDELKNKKKKNGRFGLQMPCLHVFHGNCIEDWLKNSHYCPICRFEVPIQ 235

[Close]

BLAST of PI0003842 vs. NCBI nr
Match: XP_023511497.1 (ERAD-associated E3 ubiquitin-protein ligase HRD1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 297.7 bits (761), Expect = 8.8e-77
Identity = 148/238 (62.18%), Postives = 184/238 (77.31%), Query Frame = 0


Query: 3   VEYQISRYSTMAVEVDTDLLMLTKIPKHPSPIELIGLLFTLRRRFNHFQVLENGERQLIE 62
           +E+QISRY ++ VEV TDL   ++IPKHPS IE IGL+FTLRRR +HF+V ENGER+LI+
Sbjct: 1   MEHQISRYESLVVEVLTDLPTPSRIPKHPSRIEHIGLIFTLRRRRSHFEVSENGERRLIQ 60

Query: 63  EREISKVPLCDHQFPVEIFNGDNNSLFQYLCCSFENYESKIDAEVVASVLIEQWIDIRDQ 122
           +RE  K+PLCD+QF  EIFN D++SLF  L CSF+N++ +IDAE VA  LIE+WI+IRD+
Sbjct: 61  QRETLKIPLCDNQFRAEIFNRDDDSLFHSLSCSFQNFKPEIDAEPVADALIERWIEIRDE 120

Query: 123 VNSRSCDKIFSMAVNLTITDVVVRYVIGDDGNTFGGEDFSVVPACDKTVKKMLKRLEMRD 182
            NSRS D++F M VNLTI  +V RY IG      GG+   +VPAC   V KML+RL+  D
Sbjct: 121 ANSRSSDRVFPMTVNLTIWHIVARYEIGVGS---GGDGCGMVPACGGAVAKMLRRLKRED 180

Query: 183 EDKRICVICLDEV--KKKKNGR-CLEMPCLHVFHGECIENWLKNSHCCPICRFEMPVK 238
           E   ICVICLDE+  KKKKNGR  L+MPCLHVFHG CIE+WLKNSH CPICRFE+P++
Sbjct: 181 EVDGICVICLDELKNKKKKNGRFTLQMPCLHVFHGNCIEDWLKNSHYCPICRFEVPIQ 235

[Close]

BLAST of PI0003842 vs. NCBI nr
Match: XP_022952293.1 (ERAD-associated E3 ubiquitin-protein ligase HRD1-like [Cucurbita moschata] >KAG7011584.1 HRD1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 295.8 bits (756), Expect = 3.3e-76
Identity = 147/238 (61.76%), Postives = 183/238 (76.89%), Query Frame = 0


Query: 3   VEYQISRYSTMAVEVDTDLLMLTKIPKHPSPIELIGLLFTLRRRFNHFQVLENGERQLIE 62
           +E+QISRY  + VEV TDL   ++IPKHPS IE IGL+FTLRRR +HF+V ENGER+LI+
Sbjct: 1   MEHQISRYENLVVEVLTDLPTPSRIPKHPSRIEHIGLIFTLRRRRSHFEVSENGERRLIQ 60

Query: 63  EREISKVPLCDHQFPVEIFNGDNNSLFQYLCCSFENYESKIDAEVVASVLIEQWIDIRDQ 122
           +RE  K+PLCD+QF  EIFN D++SLF  L CSF+N++ +IDAE VA  LIE+WI+IRD+
Sbjct: 61  QRETLKIPLCDNQFRAEIFNRDDDSLFHSLSCSFQNFKPEIDAEPVADALIERWIEIRDE 120

Query: 123 VNSRSCDKIFSMAVNLTITDVVVRYVIGDDGNTFGGEDFSVVPACDKTVKKMLKRLEMRD 182
            NSRS D++F M VNLT+  +V RY IG      GG+   +VPAC   V KML+RL+  D
Sbjct: 121 ANSRSSDRVFPMTVNLTMWHIVARYEIGVGS---GGDGCGMVPACGGAVAKMLRRLKRED 180

Query: 183 EDKRICVICLDEV--KKKKNGRC-LEMPCLHVFHGECIENWLKNSHCCPICRFEMPVK 238
           E   ICVICLDE+  KKKKNGR  L+MPCLHVFHG CIE+WLKNSH CPICRFE+P++
Sbjct: 181 EVDGICVICLDELKNKKKKNGRFGLQMPCLHVFHGNCIEDWLKNSHYCPICRFEVPIQ 235

[Close]

BLAST of PI0003842 vs. TAIR 10
Match: AT3G14970.1 (RING/U-box superfamily protein )

HSP 1 Score: 73.6 bits (179), Expect = 2.5e-13
Identity = 47/157 (29.94%), Postives = 84/157 (53.50%), Query Frame = 0


Query: 80  IFNGDNNSLFQYLCCSFENYESKIDAEVVASVLIEQWIDIRDQVNSRSCDKIFSM-AVNL 139
           +F+GD   +   +C   +  ++    +VV   LI+  +D+      + CDKI ++  V++
Sbjct: 74  LFSGDREQIQVIVCHILDLIKAPRYIDVVTE-LIDAILDL------KKCDKISNVKEVDV 133

Query: 140 TITDVVVRYVIGDDGNTFGGEDFSVVPACDKTVKKMLKRLEMRDEDKRICVICLDEVKKK 199
            I  +V R+   DD +       +V PA ++ V++ L+ + +  E++  CVIC+D+++  
Sbjct: 134 VINVIVWRFPDDDDDDV--DVRLAVAPASNEAVEQHLETVVV--ENESWCVICMDKIRIG 193

Query: 200 KNGRCLEMPCLHVFHGECIENWLKNSHCCPICRFEMP 236
            +     MPC HVFH  C E+WL+ S  CP+CR   P
Sbjct: 194 LDVAAGRMPCSHVFHRTCGEDWLRCSGICPVCRAMFP 219

[Close]

BLAST of PI0003842 vs. TAIR 10
Match: AT3G15740.1 (RING/U-box superfamily protein )

HSP 1 Score: 70.1 bits (170), Expect = 2.8e-12
Identity = 38/99 (38.38%), Postives = 53/99 (53.54%), Query Frame = 0


Query: 145 VRYVIGDDGNTFGGED--------FSVVPACDKTVKKMLKRLEMRDEDKRICVICLDEVK 204
           V+  IG   + F GED         +V PA D+ V+  L+ L +  E++  CVIC+D ++
Sbjct: 127 VQVKIGVTVSRFPGEDDHVDVQVQLAVAPANDEAVEMHLETLVV--ENEGYCVICMDNIR 186

Query: 205 KKKNGRCLEMPCLHVFHGECIENWLKNSHCCPICRFEMP 236
              +     MPC HVFH  C E WL+NS  CP+CR   P
Sbjct: 187 VGSDVEAGRMPCSHVFHRTCGEEWLRNSGICPVCRALFP 223

[Close]

BLAST of PI0003842 vs. TAIR 10
Match: AT1G21960.1 (RING/U-box superfamily protein )

HSP 1 Score: 67.0 bits (162), Expect = 2.3e-11
Identity = 41/157 (26.11%), Postives = 77/157 (49.04%), Query Frame = 0


Query: 89  FQYLCCSFENYESKIDAEVVASVLIEQWIDI-RDQVNSRSCDKI--FSMAVNLTITDVVV 148
           ++ +  S+ N   K++  + ++ L EQ++DI   Q+  R C  I     +V  T+  +  
Sbjct: 49  YEGVISSYPNDSKKLNDWLYSAGLSEQYVDIANSQLTERICKSISMVDSSVCATLVYMYC 108

Query: 149 RYVIGDDGNTFGGEDFSVV---PA----CDKTVKKMLKRLEMRDEDKRICVICLDEVKKK 208
           + ++    ++F     ++    PA     D+  +K+  + +  + D  +C ICL+E +K 
Sbjct: 109 KVIVPPRIDSFDETTNNIELIRPANKLVVDRLARKVYNKKKKSNSDASVCTICLEEFEKG 168

Query: 209 KNGRCLEMPCLHVFHGECIENWLKNSHCCPICRFEMP 236
           +    + +PC H F   CI  W    H CP+CRFE+P
Sbjct: 169 E--IVVTLPCGHEFDDGCIGKWFLKDHVCPLCRFELP 203

[Close]

BLAST of PI0003842 vs. TAIR 10
Match: AT1G14200.1 (RING/U-box superfamily protein )

HSP 1 Score: 66.6 bits (161), Expect = 3.1e-11
Identity = 33/78 (42.31%), Postives = 44/78 (56.41%), Query Frame = 0


Query: 165 PACDKTVKKMLKRLEMRDEDK--RICVICLDEVKKKKNGRCLEMPCLHVFHGECIENWLK 224
           PA    V+ M + +   D++K    C ICLDE    K     EMPC H FH +C+E WL 
Sbjct: 84  PALKSEVENMPRVVIGEDKEKYGGSCAICLDE--WSKGDVAAEMPCKHKFHSKCVEEWLG 143

Query: 225 NSHCCPICRFEMPVKFLE 241
               CP+CR+EMPV+ +E
Sbjct: 144 RHATCPMCRYEMPVEEVE 159

[Close]

BLAST of PI0003842 vs. TAIR 10
Match: AT1G26800.1 (RING/U-box superfamily protein )

HSP 1 Score: 65.9 bits (159), Expect = 5.2e-11
Identity = 28/49 (57.14%), Postives = 34/49 (69.39%), Query Frame = 0


Query: 188 CVICLDEVKKKKNGRCLEMPCLHVFHGECIENWLKNSHCCPICRFEMPV 237
           CVICL+E K ++  +  EMPC H FHG CIE WL     CP+CR+EMPV
Sbjct: 113 CVICLEEWKSEETVK--EMPCKHRFHGGCIEKWLGFHGSCPVCRYEMPV 159

The following BLAST results are available for this feature:

BLAST of PI0003842 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LQX2	7.4e-10	57.14	E3 ubiquitin-protein ligase MPSR1 OS=Arabidopsis thaliana OX=3702 GN=MPSR1 PE=1 ...	[more]
Q9SNB6	2.1e-09	39.19	E3 ubiquitin-protein ligase RDUF1 OS=Arabidopsis thaliana OX=3702 GN=RDUF1 PE=1 ...	[more]
Q9P0P0	2.8e-09	37.66	E3 ubiquitin-protein ligase RNF181 OS=Homo sapiens OX=9606 GN=RNF181 PE=1 SV=1	[more]
Q3T0W3	2.4e-08	36.36	E3 ubiquitin-protein ligase RNF181 OS=Bos taurus OX=9913 GN=RNF181 PE=2 SV=1	[more]
Q08109	5.3e-08	36.11	ERAD-associated E3 ubiquitin-protein ligase HRD1 OS=Saccharomyces cerevisiae (st...	[more]

BLAST of PI0003842 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L236	2.2e-113	84.68	RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G419590 P...	[more]
A0A5A7SNF1	6.3e-113	82.98	RING finger protein 141-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca...	[more]
A0A1S4E0Q9	6.3e-113	82.98	RING finger protein 141-like OS=Cucumis melo OX=3656 GN=LOC103495859 PE=4 SV=1	[more]
A0A6J1GK53	1.6e-76	61.76	ERAD-associated E3 ubiquitin-protein ligase HRD1-like OS=Cucurbita moschata OX=3...	[more]
A0A6J1I8N9	1.5e-74	60.92	ERAD-associated E3 ubiquitin-protein ligase HRD1-like OS=Cucurbita maxima OX=366...	[more]

BLAST of PI0003842 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_011654386.1	4.5e-113	84.68	RING finger protein 141 [Cucumis sativus] >KGN54667.1 hypothetical protein Csa_0...	[more]
XP_016901804.1	1.3e-112	82.98	PREDICTED: RING finger protein 141-like [Cucumis melo] >KAA0025781.1 RING finger...	[more]
KAG6571896.1	6.7e-77	62.61	E3 ubiquitin-protein ligase MPSR1, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_023511497.1	8.8e-77	62.18	ERAD-associated E3 ubiquitin-protein ligase HRD1-like [Cucurbita pepo subsp. pep...	[more]
XP_022952293.1	3.3e-76	61.76	ERAD-associated E3 ubiquitin-protein ligase HRD1-like [Cucurbita moschata] >KAG7...	[more]

BLAST of PI0003842 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G14970.1	2.5e-13	29.94	RING/U-box superfamily protein	[more]
AT3G15740.1	2.8e-12	38.38	RING/U-box superfamily protein	[more]
AT1G21960.1	2.3e-11	26.11	RING/U-box superfamily protein	[more]
AT1G14200.1	3.1e-11	42.31	RING/U-box superfamily protein	[more]
AT1G26800.1	5.2e-11	57.14	RING/U-box superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR011016	Zinc finger, RING-CH-type	SMART	SM00744	ringv_2	coord: 187..231 e-value: 0.0042 score: 10.4
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 188..230 e-value: 1.3E-5 score: 34.7
IPR001841	Zinc finger, RING-type	PFAM	PF13639	zf-RING_2	coord: 188..231 e-value: 6.0E-11 score: 42.5
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 188..231 score: 12.489278
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 162..237 e-value: 6.2E-16 score: 59.8
None	No IPR available	PANTHER	PTHR15710:SF63	RING/U-BOX SUPERFAMILY PROTEIN	coord: 159..235
None	No IPR available	PANTHER	PTHR15710	E3 UBIQUITIN-PROTEIN LIGASE PRAJA	coord: 159..235
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 179..237

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
PI0003842.1	PI0003842.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016567	protein ubiquitination
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0061630	ubiquitin protein ligase activity
molecular_function	GO:0008270	zinc ion binding