PI0000263 (gene) Melon (PI 482460) v1

Overview
NamePI0000263
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
DescriptionL-ascorbate oxidase
Locationchr08: 21309169 .. 21311359 (+)
RNA-Seq ExpressionPI0000263
SyntenyPI0000263
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTTCGTTGTTACAAAGTAGACAATACCTCTTAATTCTTCTCTCCTGCTTCTTGTTCTTATTACAATCTCCAACTGCAGAATCAAGAACTCTGAACTATAAATGGGAAGTGAAATCCGAATTGAAGTCCCCCGACTGCTTTAAAAAACTTACCATCACAATTAATGGCCGATCTCCAGGTCCCACAATCTACGCCCACCAAGGTGACACCGTCATCGTTGAGCTCAAGAACAGCCTCAATACCGAAAACGTCGCAATCCACTGGCATGGAATCCGACAGGTACCCACAAAATTTTCATCTCTCTCCATATACTTCTTTGATAATTTGATAATTTGTTTTTTCTAACGTGTTTGTATTTTTGAAGATCGGAACACCGTGGAGCGATGGAACAGAAGGTGTCACTCAATGTCCTATTTTGCCTGGTGAAACCTTCAGATATCAGTTTGTTGTAGACAGAGTAAGAGATACTGGACTCGCTGGTTTTGTTTTGTGCCTCTCAAAAACTCCATTAATTTGAGTTTGACCTTTGTGAATTTTGAAATCTGCAGCCAGGAACTTATCTATACCATGCTCATTATGGTATGCAAAGAGAAGATGGTCTCTATGGTTCCATTGTGGTTTGGGTTCCAGAAGGACAAACGGAGCCTTTTTCCTATGATCGTGATCGTAATCTCATCCTCACAGATTGGTATCATAAAAGCAGCAACGAACACGCCGCTGGATTGGCTACCCCTGGCTCTGGATTCACCTGGGTGGGAGAGCCTGATTCACTGTTGATTCAAGGAAGAGGGAGATTCAATTGCTCTGTTCTGGGATCTTCCAGTGGTACCTGCAATTCATCCAGCACAGAATGTGTTCTTTATCCATTGACAGTCGTACCGGGAAAAACTTACCGATTAAGGGTCTCTAGTCTCACTTCTCTTTCAGCTCTCAGTTTCCAAATTGAGGTAATTACAAGTTCATCTACTTAATTTCATGTACTCTCCTAGAAATTCTTCCTAAATGGCTTAAACTGATAAACTCTCTGTTTTGGAAACAGGGTCACACCATGACTGTGGTTGAAGCTGATGGGCATTACGTTGAGCCATTTCAAGTAAACAATTTGTTCATTTACTCAGGAGAAACATATTCTGTTTTGATCAAAGCCGATCAAGACCCTTCTAGAAACTACTGGATCACAACCAATGTAGTGAGCCGATCAAGAACCACCCCCCCTGGTTTAGCCATTTTCAATTACTACCCAAATCACCAGAGTAAACTCCCTCCGACAACTCCTCCAGCACCACCTGCTTGGAACGATGTTCCTCCCAGATTGGCTCAAAGTCAAGCCATCAAAGCCCGGCAAGGTTTTATCCACGCGCCGCCCAAAGCCGCCGATAAAGTGATCGTGCTTCTCAACACCCAAAACACCGTGAATGGCCGTCGCCGGTGGTCGTTAAATAATGTTTCCTTCAACATGCCTCATACCCCTTATCTTATTGCTCTAAAGCACAATCTTCTCCATACATTCAGCCAGGAAAAACCACCCACTGGATACGATTTCAAGAACTACGACATTTTCAATCCGGCGCCCAACCCTGAAGCGACGGTGAGCGACGCCATATTCCGGTTGGATTTCAATTCGACTGTGGACATCATACTACAGAACGCCAATATGATGAGCCCAAACAACAGCGAAACACATCCTTGGCATTTGCACGGACACGATTTCTGGGTGTTGGGGTACGGAGAAGGGAAATTCGATATCTATAACGATCCAAGCAAATACAATTTGGAGAATCCGATCATGAAGAACACGGTGCCGTTGCATCCATATGGATGGACCGCTTTGAGATTTGTGGCGGATAATCCCGGGGTTTGGGCATTCCACTGTCACATCGACGCCCATTTCTTTATGGGAATGGGTGTCGTTTTTGAAGAAGGGATCGAGAGGGTTGGGAAGTTGCCTACTTCCATTATGGGATGCGGCGAGAGCAAGAGGTTCTTGAGACCTTAACCCATTTCCATCCCAATTATTATTCTTTAAAGAAACATGAAGGATATTCTTTATGTTTAGCCAAATTCTTTGTACTGATAGAGTCACTATGAGTGTGTAGGTAAACGTTGTTGCTACTAAATATAATCCTCGTTCCCTACGGAAGAAATTGTAATAGTTATTGAAATAATTTAAATAAGAATACAATTGTTA

mRNA sequence

ATGGCTTCGTTGTTACAAAGTAGACAATACCTCTTAATTCTTCTCTCCTGCTTCTTGTTCTTATTACAATCTCCAACTGCAGAATCAAGAACTCTGAACTATAAATGGGAAGTGAAATCCGAATTGAAGTCCCCCGACTGCTTTAAAAAACTTACCATCACAATTAATGGCCGATCTCCAGGTCCCACAATCTACGCCCACCAAGGTGACACCGTCATCGTTGAGCTCAAGAACAGCCTCAATACCGAAAACGTCGCAATCCACTGGCATGGAATCCGACAGATCGGAACACCGTGGAGCGATGGAACAGAAGGTGTCACTCAATGTCCTATTTTGCCTGGTGAAACCTTCAGATATCAGTTTGTTGTAGACAGACCAGGAACTTATCTATACCATGCTCATTATGGTATGCAAAGAGAAGATGGTCTCTATGGTTCCATTGTGGTTTGGGTTCCAGAAGGACAAACGGAGCCTTTTTCCTATGATCGTGATCGTAATCTCATCCTCACAGATTGGTATCATAAAAGCAGCAACGAACACGCCGCTGGATTGGCTACCCCTGGCTCTGGATTCACCTGGGTGGGAGAGCCTGATTCACTGTTGATTCAAGGAAGAGGGAGATTCAATTGCTCTGTTCTGGGATCTTCCAGTGGTACCTGCAATTCATCCAGCACAGAATGTGTTCTTTATCCATTGACAGTCGTACCGGGAAAAACTTACCGATTAAGGGTCTCTAGTCTCACTTCTCTTTCAGCTCTCAGTTTCCAAATTGAGGGTCACACCATGACTGTGGTTGAAGCTGATGGGCATTACGTTGAGCCATTTCAAGTAAACAATTTGTTCATTTACTCAGGAGAAACATATTCTGTTTTGATCAAAGCCGATCAAGACCCTTCTAGAAACTACTGGATCACAACCAATGTAGTGAGCCGATCAAGAACCACCCCCCCTGGTTTAGCCATTTTCAATTACTACCCAAATCACCAGAGTAAACTCCCTCCGACAACTCCTCCAGCACCACCTGCTTGGAACGATGTTCCTCCCAGATTGGCTCAAAGTCAAGCCATCAAAGCCCGGCAAGGTTTTATCCACGCGCCGCCCAAAGCCGCCGATAAAGTGATCGTGCTTCTCAACACCCAAAACACCGTGAATGGCCGTCGCCGGTGGTCGTTAAATAATGTTTCCTTCAACATGCCTCATACCCCTTATCTTATTGCTCTAAAGCACAATCTTCTCCATACATTCAGCCAGGAAAAACCACCCACTGGATACGATTTCAAGAACTACGACATTTTCAATCCGGCGCCCAACCCTGAAGCGACGGTGAGCGACGCCATATTCCGGTTGGATTTCAATTCGACTGTGGACATCATACTACAGAACGCCAATATGATGAGCCCAAACAACAGCGAAACACATCCTTGGCATTTGCACGGACACGATTTCTGGGTGTTGGGGTACGGAGAAGGGAAATTCGATATCTATAACGATCCAAGCAAATACAATTTGGAGAATCCGATCATGAAGAACACGGTGCCGTTGCATCCATATGGATGGACCGCTTTGAGATTTGTGGCGGATAATCCCGGGGTTTGGGCATTCCACTGTCACATCGACGCCCATTTCTTTATGGGAATGGGTGTCGTTTTTGAAGAAGGGATCGAGAGGGTTGGGAAGTTGCCTACTTCCATTATGGGATGCGGCGAGAGCAAGAGGTTCTTGAGACCTTAACCCATTTCCATCCCAATTATTATTCTTTAAAGAAACATGAAGGATATTCTTTATGTTTAGCCAAATTCTTTGTACTGATAGAGTCACTATGAGTGTGTAGGTAAACGTTGTTGCTACTAAATATAATCCTCGTTCCCTACGGAAGAAATTGTAATAGTTATTGAAATAATTTAAATAAGAATACAATTGTTA

Coding sequence (CDS)

ATGGCTTCGTTGTTACAAAGTAGACAATACCTCTTAATTCTTCTCTCCTGCTTCTTGTTCTTATTACAATCTCCAACTGCAGAATCAAGAACTCTGAACTATAAATGGGAAGTGAAATCCGAATTGAAGTCCCCCGACTGCTTTAAAAAACTTACCATCACAATTAATGGCCGATCTCCAGGTCCCACAATCTACGCCCACCAAGGTGACACCGTCATCGTTGAGCTCAAGAACAGCCTCAATACCGAAAACGTCGCAATCCACTGGCATGGAATCCGACAGATCGGAACACCGTGGAGCGATGGAACAGAAGGTGTCACTCAATGTCCTATTTTGCCTGGTGAAACCTTCAGATATCAGTTTGTTGTAGACAGACCAGGAACTTATCTATACCATGCTCATTATGGTATGCAAAGAGAAGATGGTCTCTATGGTTCCATTGTGGTTTGGGTTCCAGAAGGACAAACGGAGCCTTTTTCCTATGATCGTGATCGTAATCTCATCCTCACAGATTGGTATCATAAAAGCAGCAACGAACACGCCGCTGGATTGGCTACCCCTGGCTCTGGATTCACCTGGGTGGGAGAGCCTGATTCACTGTTGATTCAAGGAAGAGGGAGATTCAATTGCTCTGTTCTGGGATCTTCCAGTGGTACCTGCAATTCATCCAGCACAGAATGTGTTCTTTATCCATTGACAGTCGTACCGGGAAAAACTTACCGATTAAGGGTCTCTAGTCTCACTTCTCTTTCAGCTCTCAGTTTCCAAATTGAGGGTCACACCATGACTGTGGTTGAAGCTGATGGGCATTACGTTGAGCCATTTCAAGTAAACAATTTGTTCATTTACTCAGGAGAAACATATTCTGTTTTGATCAAAGCCGATCAAGACCCTTCTAGAAACTACTGGATCACAACCAATGTAGTGAGCCGATCAAGAACCACCCCCCCTGGTTTAGCCATTTTCAATTACTACCCAAATCACCAGAGTAAACTCCCTCCGACAACTCCTCCAGCACCACCTGCTTGGAACGATGTTCCTCCCAGATTGGCTCAAAGTCAAGCCATCAAAGCCCGGCAAGGTTTTATCCACGCGCCGCCCAAAGCCGCCGATAAAGTGATCGTGCTTCTCAACACCCAAAACACCGTGAATGGCCGTCGCCGGTGGTCGTTAAATAATGTTTCCTTCAACATGCCTCATACCCCTTATCTTATTGCTCTAAAGCACAATCTTCTCCATACATTCAGCCAGGAAAAACCACCCACTGGATACGATTTCAAGAACTACGACATTTTCAATCCGGCGCCCAACCCTGAAGCGACGGTGAGCGACGCCATATTCCGGTTGGATTTCAATTCGACTGTGGACATCATACTACAGAACGCCAATATGATGAGCCCAAACAACAGCGAAACACATCCTTGGCATTTGCACGGACACGATTTCTGGGTGTTGGGGTACGGAGAAGGGAAATTCGATATCTATAACGATCCAAGCAAATACAATTTGGAGAATCCGATCATGAAGAACACGGTGCCGTTGCATCCATATGGATGGACCGCTTTGAGATTTGTGGCGGATAATCCCGGGGTTTGGGCATTCCACTGTCACATCGACGCCCATTTCTTTATGGGAATGGGTGTCGTTTTTGAAGAAGGGATCGAGAGGGTTGGGAAGTTGCCTACTTCCATTATGGGATGCGGCGAGAGCAAGAGGTTCTTGAGACCTTAA

Protein sequence

MASLLQSRQYLLILLSCFLFLLQSPTAESRTLNYKWEVKSELKSPDCFKKLTITINGRSPGPTIYAHQGDTVIVELKNSLNTENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFRYQFVVDRPGTYLYHAHYGMQREDGLYGSIVVWVPEGQTEPFSYDRDRNLILTDWYHKSSNEHAAGLATPGSGFTWVGEPDSLLIQGRGRFNCSVLGSSSGTCNSSSTECVLYPLTVVPGKTYRLRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQVNNLFIYSGETYSVLIKADQDPSRNYWITTNVVSRSRTTPPGLAIFNYYPNHQSKLPPTTPPAPPAWNDVPPRLAQSQAIKARQGFIHAPPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKHNLLHTFSQEKPPTGYDFKNYDIFNPAPNPEATVSDAIFRLDFNSTVDIILQNANMMSPNNSETHPWHLHGHDFWVLGYGEGKFDIYNDPSKYNLENPIMKNTVPLHPYGWTALRFVADNPGVWAFHCHIDAHFFMGMGVVFEEGIERVGKLPTSIMGCGESKRFLRP
Homology
[Close]
BLAST of PI0000263 vs. ExPASy Swiss-Prot
Match: P14133 (L-ascorbate oxidase OS=Cucumis sativus OX=3659 PE=1 SV=1)

HSP 1 Score: 610.9 bits (1574), Expect = 1.5e-173
Identity = 286/542 (52.77%), Postives = 371/542 (68.45%), Query Frame = 0 

Query: 33  NYKWEVKSELKSPDCFKKLTITINGRSPGPTIYAHQGDTVIVELKNSLNTENVAIHWHGI 92
           +YKW+V+    SPDC + + + ING  PGPTI A+ GD V+VEL N L+TE V IHWHGI
Sbjct: 40  HYKWDVEYMFWSPDCVENIVMGINGEFPGPTIRANAGDIVVVELTNKLHTEGVVIHWHGI 99

Query: 93  RQIGTPWSDGTEGVTQCPILPGETFRYQFVVDRPGTYLYHAHYGMQREDGLYGSIVVWVP 152
            Q GTPW+DGT  ++QC I PGETF Y+FVVD+ GTY YH H GMQR  GLYGS++V  P
Sbjct: 100 LQRGTPWADGTASISQCAINPGETFTYRFVVDKAGTYFYHGHLGMQRSAGLYGSLIVDPP 159

Query: 153 EGQTEPFSYDRDRNLILTDWYHKSSNEHAAGLATPGSGFTWVGEPDSLLIQGRGRFNCSV 212
           EG++EPF YD + NL+L+DW+H+S ++   GL++      W+GEP S+LI G+G+F+CS+
Sbjct: 160 EGRSEPFHYDEEINLLLSDWWHQSVHKQEVGLSS--KPMRWIGEPQSILINGKGQFDCSI 219

Query: 213 LGSSS---GTCNSSSTE-CVLYPLTVVPGKTYRLRVSSLTSLSALSFQIEGHTMTVVEAD 272
               +     C  S  E C  + L V P KTYR+R++S T+L++L+F I  H + VVEAD
Sbjct: 220 AAKYNQGLKQCELSGKEKCAPFILHVQPKKTYRIRIASTTALASLNFAIGNHELLVVEAD 279

Query: 273 GHYVEPFQVNNLFIYSGETYSVLIKADQDPSRNYWITTNVVSRSRTTPPGLAIFNYYPNH 332
           G+YV+PF  +++ IYSGE+YSVLI  DQ+P  NYW++  V +R   TPPGL + NY PN 
Sbjct: 280 GNYVQPFVTSDIDIYSGESYSVLITTDQNPLENYWVSIGVRARLPKTPPGLTLLNYLPNS 339

Query: 333 QSKLPPTTPPAPPAWNDVPPRLAQSQAIKARQGFIHAPPKAADKVIVLLNTQNTVNGRRR 392
            SKLP + PP  P W D       +  I A  G    PP   ++ + LLNTQN +NG  +
Sbjct: 340 ASKLPISPPPETPHWEDFDRSKNFTFRIFAAMG-SPKPPVRYNRRLFLLNTQNRINGFMK 399

Query: 393 WSLNNVSFNMPHTPYLIALKHNLLHTFSQEKPPTGYDFKNYDIFNPAPNPEATVSDAIFR 452
           W++NNVS  +P TPYL A+K  L   F+Q  PP  +   NYDI NP PNPE T  + +++
Sbjct: 400 WAINNVSLALPPTPYLAAMKMRLNTAFNQNPPPETFPL-NYDINNPPPNPETTTGNGVYK 459

Query: 453 LDFNSTVDIILQNANMMSPNNSETHPWHLHGHDFWVLGYGEGKFDIYNDPSKYNLENPIM 512
            +   TVD+ILQNANM++PN SE HPWHLHGHDFWVLGYGEGKF    D  K NL+NP +
Sbjct: 460 FNMGETVDVILQNANMLNPNMSEIHPWHLHGHDFWVLGYGEGKFYAPEDEKKLNLKNPPL 519

Query: 513 KNTVPLHPYGWTALRFVADNPGVWAFHCHIDAHFFMGMGVVFEEGIERVGKLPTSIMGCG 571
           +NTV + PYGWTA+RFVADNPGVWAFHCHI+ H  MGMGVVF EG+  VG +P   + CG
Sbjct: 520 RNTVVIFPYGWTAIRFVADNPGVWAFHCHIEPHLHMGMGVVFAEGVHMVGMIPPKALACG 577

[Close]
BLAST of PI0000263 vs. ExPASy Swiss-Prot
Match: P37064 (L-ascorbate oxidase OS=Cucurbita pepo var. melopepo OX=3665 PE=1 SV=1)

HSP 1 Score: 606.7 bits (1563), Expect = 2.8e-172
Identity = 283/550 (51.45%), Postives = 378/550 (68.73%), Query Frame = 0 

Query: 29  SRTLNYKWEVKSELKSPDCFKKLTITINGRSPGPTIYAHQGDTVIVELKNSLNTENVAIH 88
           S+  +YKWEV+    +P+C + + + ING+ PGPTI A+ GD+V+VEL N L+TE V IH
Sbjct: 1   SQIRHYKWEVEYMFWAPNCNENIVMGINGQFPGPTIRANAGDSVVVELTNKLHTEGVVIH 60

Query: 89  WHGIRQIGTPWSDGTEGVTQCPILPGETFRYQFVVDRPGTYLYHAHYGMQREDGLYGSIV 148
           WHGI Q GTPW+DGT  ++QC I PGETF Y F VD PGT+ YH H GMQR  GLYGS++
Sbjct: 61  WHGILQRGTPWADGTASISQCAINPGETFFYNFTVDNPGTFFYHGHLGMQRSAGLYGSLI 120

Query: 149 VWVPEGQTEPFSYDRDRNLILTDWYHKSSNEHAAGLATPGSGFTWVGEPDSLLIQGRGRF 208
           V  P+G+ EPF YD + NL+L+DW+H+S ++   GL++      W+GEP ++L+ GRG+F
Sbjct: 121 VDPPQGKKEPFHYDGEINLLLSDWWHQSIHKQEVGLSS--KPIRWIGEPQTILLNGRGQF 180

Query: 209 NCSVLG---SSSGTCNSSSTE-CVLYPLTVVPGKTYRLRVSSLTSLSALSFQIEGHTMTV 268
           +CS+     S+   C    +E C  Y   V P KTYR+R++S T+L+AL+F I  H + V
Sbjct: 181 DCSIAAKYDSNLEPCKLKGSESCAPYIFHVSPKKTYRIRIASTTALAALNFAIGNHQLLV 240

Query: 269 VEADGHYVEPFQVNNLFIYSGETYSVLIKADQDPSRNYWITTNVVSRSRTTPPGLAIFNY 328
           VEADG+YV+PF  +++ IYSGE+YSVLI  DQ+PS NYW++    +R   TPPGL + NY
Sbjct: 241 VEADGNYVQPFYTSDIDIYSGESYSVLITTDQNPSENYWVSVGTRARHPNTPPGLTLLNY 300

Query: 329 YPNHQSKLPPTTPPAPPAWNDVPPRLAQSQAIKARQGFIHAPPKAADKVIVLLNTQNTVN 388
            PN  SKLP + PP  PAW+D       +  I A  G    PP   ++ I LLNTQN +N
Sbjct: 301 LPNSVSKLPTSPPPQTPAWDDFDRSKNFTYRITAAMG-SPKPPVKFNRRIFLLNTQNVIN 360

Query: 389 GRRRWSLNNVSFNMPHTPYLIALKHNLLHTFSQEKPPTGYDFKNYDIFNPAPNPEATVSD 448
           G  +W++N+VS  +P TPYL A+K+NLLH F Q  PP  +  ++YDI  P  N +  + +
Sbjct: 361 GYVKWAINDVSLALPPTPYLGAMKYNLLHAFDQNPPPEVFP-EDYDIDTPPTNEKTRIGN 420

Query: 449 AIFRLDFNSTVDIILQNANMMSPNNSETHPWHLHGHDFWVLGYGEGKFDIYNDPSKYNLE 508
            +++      VD+ILQNANMM  N SETHPWHLHGHDFWVLGYG+GKF    + S  NL+
Sbjct: 421 GVYQFKIGEVVDVILQNANMMKENLSETHPWHLHGHDFWVLGYGDGKFSA-EEESSLNLK 480

Query: 509 NPIMKNTVPLHPYGWTALRFVADNPGVWAFHCHIDAHFFMGMGVVFEEGIERVGKLPTSI 568
           NP ++NTV + PYGWTA+RFVADNPGVWAFHCHI+ H  MGMGVVF EG+E+VG++PT  
Sbjct: 481 NPPLRNTVVIFPYGWTAIRFVADNPGVWAFHCHIEPHLHMGMGVVFAEGVEKVGRIPTKA 540

Query: 569 MGCGESKRFL 575
           + CG + + L
Sbjct: 541 LACGGTAKSL 545

[Close]
BLAST of PI0000263 vs. ExPASy Swiss-Prot
Match: P24792 (L-ascorbate oxidase OS=Cucurbita maxima OX=3661 GN=AAO PE=1 SV=2)

HSP 1 Score: 605.1 bits (1559), Expect = 8.1e-172
Identity = 290/568 (51.06%), Postives = 385/568 (67.78%), Query Frame = 0 

Query: 12  LILLSCFLFLLQSPTAE-SRTLNYKWEVKSELKSPDCFKKLTITINGRSPGPTIYAHQGD 71
           LIL    L L    ++E S+  +YKWEV+    +PDC + + + ING+ PGPTI A+ GD
Sbjct: 13  LILFFFGLILAFGISSEGSQIRHYKWEVEYMFWAPDCNENIVMGINGQFPGPTIRANAGD 72

Query: 72  TVIVELKNSLNTENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFRYQFVVDRPGTYL 131
           TV+VEL N L+TE V IHWHGI Q GTPW+DGT  ++QC I PGETF Y F VD PGT+ 
Sbjct: 73  TVVVELINKLHTEGVVIHWHGILQRGTPWADGTASISQCAINPGETFFYNFTVDNPGTFF 132

Query: 132 YHAHYGMQREDGLYGSIVVWVPEGQTEPFSYDRDRNLILTDWYHKSSNEHAAGLATPGSG 191
           YH H GMQR  GLYGS++V  P+G+ EPF YD + NL+L+DW+H+S ++   GL++    
Sbjct: 133 YHGHLGMQRSAGLYGSLIVDPPQGKKEPFHYDGEINLLLSDWWHQSIHKQEVGLSS--KP 192

Query: 192 FTWVGEPDSLLIQGRGRFNCSVLG---SSSGTCNSSSTE-CVLYPLTVVPGKTYRLRVSS 251
             W+GEP ++L+ GRG+F+CS+     S+   C    +E C  Y   V+P KTYR+R++S
Sbjct: 193 IRWIGEPQTILLNGRGQFDCSIAAKYDSNLEPCKLKGSEPCAPYIFHVMPKKTYRIRIAS 252

Query: 252 LTSLSALSFQIEGHTMTVVEADGHYVEPFQVNNLFIYSGETYSVLIKADQDPSRNYWITT 311
            T+L+AL+F I  H + VVEADG+YV+PF  +++ IYSGE+YSVLI  DQ+PS NYW++ 
Sbjct: 253 TTALAALNFAIGNHPLLVVEADGNYVQPFYTSDIDIYSGESYSVLITTDQNPSENYWVSV 312

Query: 312 NVVSRSRTTPPGLAIFNYYPNHQSKLPPTTPPAPPAWNDVPPRLAQSQAIKARQGFIHAP 371
               R   TPPGL + NY PN  SKLP + PP  PAW+D       +  I A  G    P
Sbjct: 313 GTRGRHPNTPPGLTLLNYLPNSVSKLPTSPPPETPAWDDFDRSKNFTYRITAAMG-SPKP 372

Query: 372 PKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKHNLLHTFSQEKPPTGYDF 431
           P  +++ I LLNTQN +NG  +W++N+VS  +P TPYL A+K NLLH F Q  PP  +  
Sbjct: 373 PVKSNRRIFLLNTQNVINGYVKWAINDVSLALPPTPYLGAMKFNLLHAFDQNPPPEVFP- 432

Query: 432 KNYDIFNPAPNPEATVSDAIFRLDFNSTVDIILQNANMMSPNNSETHPWHLHGHDFWVLG 491
           ++YDI  P  N +  + + +++      VD+ILQNANMM  N SE HPWHLHGHDFWVLG
Sbjct: 433 EDYDIDTPPTNEKTKIGNGVYQFKIGEIVDVILQNANMMKENLSEIHPWHLHGHDFWVLG 492

Query: 492 YGEGKFDIYNDPSKYNLENPIMKNTVPLHPYGWTALRFVADNPGVWAFHCHIDAHFFMGM 551
           YG+GKF    + S  NL+NP ++NTV + PYGWTA+RFVADNPGVWAFHCHI+ H  MGM
Sbjct: 493 YGDGKFTA-EEESSLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVWAFHCHIEPHLHMGM 552

Query: 552 GVVFEEGIERVGKLPTSIMGCGESKRFL 575
           GVVF EG+E+VG++PT  + CG + + L
Sbjct: 553 GVVFAEGVEKVGRIPTKALACGGTAKSL 575

[Close]
BLAST of PI0000263 vs. ExPASy Swiss-Prot
Match: M4DUF2 (L-ascorbate oxidase OS=Brassica rapa subsp. pekinensis OX=51351 GN=AO PE=2 SV=1)

HSP 1 Score: 601.7 bits (1550), Expect = 8.9e-171
Identity = 283/554 (51.08%), Postives = 371/554 (66.97%), Query Frame = 0 

Query: 26  TAESRTLNYKWEVKSELKSPDCFKKLTITINGRSPGPTIYAHQGDTVIVELKNSLNTENV 85
           TA +    Y WEV+ +   PDC + + + +NG+ PGPTI+A  GDT++V L N L TE +
Sbjct: 15  TASAAVREYAWEVEYKFGWPDCKEGMVMAVNGQFPGPTIHALAGDTIVVHLTNKLATEGL 74

Query: 86  AIHWHGIRQIGTPWSDGTEGVTQCPILPGETFRYQFVVDRPGTYLYHAHYGMQREDGLYG 145
            IHWHGIRQ+G+PW+DG  GVTQC I PGETF Y F VD+PGT+ YH HYGMQR  GLYG
Sbjct: 75  VIHWHGIRQLGSPWADGAAGVTQCAISPGETFTYNFTVDKPGTHFYHGHYGMQRSAGLYG 134

Query: 146 SIVVWVPEGQTEPFSYDRDRNLILTDWYHKSSNEHAAGLATPGSGFTWVGEPDSLLIQGR 205
           S+++ V +G+ EP  YD + NL+L+DW+H+       GL++      W+GE  S+LI GR
Sbjct: 135 SLIIDVAKGKKEPLRYDGEFNLLLSDWWHEDVLSQEIGLSS--RPMRWIGEAQSILINGR 194

Query: 206 GRFNCSVLGSSSGTCNSSST-----ECVLYPLTVVPGKTYRLRVSSLTSLSALSFQIEGH 265
           G+FNCS+    S T   + T     +C    L V P KTYR+R++S T+L++L+F ++GH
Sbjct: 195 GQFNCSLAAQFSSTSLPTCTFKEGDQCAPQRLHVEPNKTYRIRLASSTALASLNFAVQGH 254

Query: 266 TMTVVEADGHYVEPFQVNNLFIYSGETYSVLIKADQDPSRNYWITTNVVSRSRTTPPGLA 325
            + VVEADG+Y+ PF  +++ IYSGETYSVL+  DQDPS+NY+IT  V  R   TPP L 
Sbjct: 255 KLVVVEADGNYITPFTTDDIDIYSGETYSVLLTTDQDPSQNYYITAGVRGRKPNTPPALT 314

Query: 326 IFNYYPNHQSKLPPTTPPAPPAWNDVPPRLAQSQAIKARQGFIHAPPKAADKVIVLLNTQ 385
           + NY     S+LP + PP  P WND       S+ I A  G   +PP+  D+ ++LLNTQ
Sbjct: 315 VLNYVTAPSSQLPTSPPPETPRWNDFDRSKNFSKKIFAAMG-SPSPPETFDERLILLNTQ 374

Query: 386 NTVNGRRRWSLNNVSFNMPHTPYLIALKHNLLHTFSQEKPPTGYDFKNYDIFNPAPNPEA 445
           N + G  +W++NNVS  +P TPYL ++K+NL   F++  PP  Y   +YDI  P  N  A
Sbjct: 375 NLIEGFTKWAINNVSLAVPGTPYLGSVKYNLRTGFNRSSPPKDYPV-DYDIMTPPRNRNA 434

Query: 446 TVSDAIFRLDFNSTVDIILQNANMMSPNNSETHPWHLHGHDFWVLGYGEGKFDIYNDPSK 505
              +      FN TVD+ILQNAN ++ N SE HPWHLHGHDFWVLGYGEGKF    D   
Sbjct: 435 KQGNVSCVFPFNVTVDVILQNANGLNANASEIHPWHLHGHDFWVLGYGEGKFKPGVDEKT 494

Query: 506 YNLENPIMKNTVPLHPYGWTALRFVADNPGVWAFHCHIDAHFFMGMGVVFEEGIERVGKL 565
           YNL+NP ++NTV L+PYGWTALRFV DNPGVW FHCHI+ H  MGMGVVF EG+ R+GK+
Sbjct: 495 YNLKNPPLRNTVALYPYGWTALRFVTDNPGVWFFHCHIEPHLHMGMGVVFAEGLNRIGKV 554

Query: 566 PTSIMGCGESKRFL 575
           P   +GCG +K+FL
Sbjct: 555 PDEALGCGLTKQFL 564

[Close]
BLAST of PI0000263 vs. ExPASy Swiss-Prot
Match: Q40588 (L-ascorbate oxidase OS=Nicotiana tabacum OX=4097 GN=AAO PE=2 SV=1)

HSP 1 Score: 580.9 bits (1496), Expect = 1.6e-164
Identity = 292/582 (50.17%), Postives = 383/582 (65.81%), Query Frame = 0 

Query: 1   MASLLQSRQYLLILLSCFLFLLQSPTAESRTLNYKWEVKSELKSPDCFKKLTITINGRSP 60
           MASL     +LL L+   L   +S  A ++T ++KW+V+    SPD  + + + ING+ P
Sbjct: 1   MASLGFLFFFLLPLILLELSSSRSVMA-AKTRHFKWDVEYIHWSPDGEESVVMGINGQFP 60

Query: 61  GPTIYAHQGDTVIVELKNSLNTENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFRYQ 120
           GPTI A  GDTV V L N L+TE V IHWHGIRQIGTPW+DGT  ++QC I PGETF Y+
Sbjct: 61  GPTIRAKAGDTVAVHLTNKLHTEGVVIHWHGIRQIGTPWADGTAAISQCAINPGETFLYR 120

Query: 121 FVVDRPGTYLYHAHYGMQREDGLYGSIVVWVPEGQTEPFSYDRDRNLILTDWYHKSSNEH 180
           F VD+ GTY YH HYGMQR  GLYGS++V V EG+ EPF YD + NL+L+DW+HK S+E 
Sbjct: 121 FKVDKAGTYFYHGHYGMQRSAGLYGSLIVEVGEGEKEPFHYDGEFNLLLSDWWHKGSHEQ 180

Query: 181 AAGLATPGSGFTWVGEPDSLLIQGRGRFNCSVLGSSSGTCNSSSTECVL--------YPL 240
              L++  +   W+GEP +LL+ GRG++NCS+    S        +C L          L
Sbjct: 181 EVDLSS--NPLRWIGEPQTLLLNGRGQYNCSLAARFS---KPPLPQCKLRGGEQYAPQIL 240

Query: 241 TVVPGKTYRLRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQVNNLFIYSGETYSVLI 300
            V P K YRLRV+S T+L +LS  I GH M VVEADG+YV+PF V ++ IYSGE+YSVL 
Sbjct: 241 RVRPNKIYRLRVASTTALGSLSLAIGGHKMVVVEADGNYVQPFSVQDMDIYSGESYSVLF 300

Query: 301 KADQDPSRNYWITTNVVSRSRTTPPGLAIFNYYPNHQSKLPPTTPPAPPAWNDVPPRLAQ 360
           K DQDP++NYWI+ NV  R   TP GL + NY PN  SK P   PP  P WND     + 
Sbjct: 301 KTDQDPTKNYWISINVRGREPKTPQGLTLLNYLPNSASKFPTLPPPIAPLWNDYNHSKSF 360

Query: 361 SQAIKARQGFIHAPPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKHNLL 420
           S  I A  G    PP+   + I+LLNTQN ++G  +W++NNVS  +P   YL ++++  +
Sbjct: 361 SNKIFALMGSPKPPPQ-NHRRIILLNTQNKIDGYTKWAINNVSLVLPTQLYLGSIRYG-I 420

Query: 421 HTFSQEKPPTGYDFKNYDIFNPAPNPEATVSDAIFRLDFNSTVDIILQNANMMSPNNSET 480
           + F  + PP  +  K+YD+   APN  +T  + ++ L FN+T+DIILQNAN ++ + SE 
Sbjct: 421 NAFDTKPPPDNFP-KDYDVLKQAPNSNSTYGNGVYMLKFNTTIDIILQNANALAKDVSEI 480

Query: 481 HPWHLHGHDFWVLGYGEGKFDIYNDPSKYNLENPIMKNTVPLHPYGWTALRFVADNPGVW 540
           HPWHLHGHDFWVLGYGEGKF    D  K+NL+NP ++NT  + P+GWTALRFV DNPGVW
Sbjct: 481 HPWHLHGHDFWVLGYGEGKFS-EKDVKKFNLKNPPLRNTAVIFPFGWTALRFVTDNPGVW 540

Query: 541 AFHCHIDAHFFMGMGVVFEEGIERVGKLPTSIMGCGESKRFL 575
           AFHCHI+ H  MGMGV+F EG+  V K+P   + CG + + L
Sbjct: 541 AFHCHIEPHLHMGMGVIFAEGVHLVKKIPKEALACGLTGKML 572

[Close]
BLAST of PI0000263 vs. ExPASy TrEMBL
Match: A0A5A7SYW4 (L-ascorbate oxidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G002620 PE=3 SV=1)

HSP 1 Score: 1192.2 bits (3083), Expect = 0.0e+00
Identity = 565/576 (98.09%), Postives = 570/576 (98.96%), Query Frame = 0 

Query: 1   MASLLQSRQYLLILLSCFLFLLQSPTAESRTLNYKWEVKSELKSPDCFKKLTITINGRSP 60
           MASLLQ +QY L+LLS FLFLLQS TAESRTLNYKWEVKSELKSPDCFKKLTITINGRSP
Sbjct: 1   MASLLQRKQYFLLLLSSFLFLLQSQTAESRTLNYKWEVKSELKSPDCFKKLTITINGRSP 60

Query: 61  GPTIYAHQGDTVIVELKNSLNTENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFRYQ 120
           GPTIYAHQGDTVIVELKNSL+TENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFRYQ
Sbjct: 61  GPTIYAHQGDTVIVELKNSLHTENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFRYQ 120

Query: 121 FVVDRPGTYLYHAHYGMQREDGLYGSIVVWVPEGQTEPFSYDRDRNLILTDWYHKSSNEH 180
           FVVDRPGTYLYHAHYGMQREDGLYGSIVVWVPEGQTEPFSYD DRNLILTDWYHKSSNEH
Sbjct: 121 FVVDRPGTYLYHAHYGMQREDGLYGSIVVWVPEGQTEPFSYDHDRNLILTDWYHKSSNEH 180

Query: 181 AAGLATPGSGFTWVGEPDSLLIQGRGRFNCSVLGSSSGTCNSSSTECVLYPLTVVPGKTY 240
           AAGLATPGSGFTWVGEPDSLLIQGRGRFNCSVLGSSSGTCNSSSTECVLYPLTVVPGKTY
Sbjct: 181 AAGLATPGSGFTWVGEPDSLLIQGRGRFNCSVLGSSSGTCNSSSTECVLYPLTVVPGKTY 240

Query: 241 RLRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQVNNLFIYSGETYSVLIKADQDPSR 300
           RLRVSSLTSLSALSFQIEGH+MTVVEADGHYVEPFQVNNLFIYSGETYSVLIKADQDPSR
Sbjct: 241 RLRVSSLTSLSALSFQIEGHSMTVVEADGHYVEPFQVNNLFIYSGETYSVLIKADQDPSR 300

Query: 301 NYWITTNVVSRSRTTPPGLAIFNYYPNHQSKLPPTTPPAPPAWNDVPPRLAQSQAIKARQ 360
           NYWITTNVVSRSRTTPPGLAIFNYYPNHQSK PPTTPPAPPAWNDVPPRLAQSQAIKARQ
Sbjct: 301 NYWITTNVVSRSRTTPPGLAIFNYYPNHQSKRPPTTPPAPPAWNDVPPRLAQSQAIKARQ 360

Query: 361 GFIHAPPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKHNLLHTFSQEKP 420
           GFIHAPPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKHNLLHTFSQEKP
Sbjct: 361 GFIHAPPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKHNLLHTFSQEKP 420

Query: 421 PTGYDFKNYDIFNPAPNPEATVSDAIFRLDFNSTVDIILQNANMMSPNNSETHPWHLHGH 480
           PTGYDFKNYDIFNPAPNPEATVSDAIFRLDFNSTVD+ILQNANMMSPNNSETHPWHLHGH
Sbjct: 421 PTGYDFKNYDIFNPAPNPEATVSDAIFRLDFNSTVDLILQNANMMSPNNSETHPWHLHGH 480

Query: 481 DFWVLGYGEGKFDIYNDPSKYNLENPIMKNTVPLHPYGWTALRFVADNPGVWAFHCHIDA 540
           DFWVLGYGEGKFDIYNDPSKYNLENPIMKNTVPLHPYGWTALRFVADNPGVWAFHCHIDA
Sbjct: 481 DFWVLGYGEGKFDIYNDPSKYNLENPIMKNTVPLHPYGWTALRFVADNPGVWAFHCHIDA 540

Query: 541 HFFMGMGVVFEEGIERVGKLPTSIMGCGESKRFLRP 577
           HFFMGMGVVFEEGIERVGKLPTSIMGCGESKRFLRP
Sbjct: 541 HFFMGMGVVFEEGIERVGKLPTSIMGCGESKRFLRP 576

[Close]
BLAST of PI0000263 vs. ExPASy TrEMBL
Match: A0A1S3B151 (L-ascorbate oxidase OS=Cucumis melo OX=3656 GN=LOC103484994 PE=3 SV=1)

HSP 1 Score: 1192.2 bits (3083), Expect = 0.0e+00
Identity = 565/576 (98.09%), Postives = 570/576 (98.96%), Query Frame = 0 

Query: 1   MASLLQSRQYLLILLSCFLFLLQSPTAESRTLNYKWEVKSELKSPDCFKKLTITINGRSP 60
           MASLLQ +QY L+LLS FLFLLQS TAESRTLNYKWEVKSELKSPDCFKKLTITINGRSP
Sbjct: 1   MASLLQRKQYFLLLLSSFLFLLQSQTAESRTLNYKWEVKSELKSPDCFKKLTITINGRSP 60

Query: 61  GPTIYAHQGDTVIVELKNSLNTENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFRYQ 120
           GPTIYAHQGDTVIVELKNSL+TENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFRYQ
Sbjct: 61  GPTIYAHQGDTVIVELKNSLHTENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFRYQ 120

Query: 121 FVVDRPGTYLYHAHYGMQREDGLYGSIVVWVPEGQTEPFSYDRDRNLILTDWYHKSSNEH 180
           FVVDRPGTYLYHAHYGMQREDGLYGSIVVWVPEGQTEPFSYD DRNLILTDWYHKSSNEH
Sbjct: 121 FVVDRPGTYLYHAHYGMQREDGLYGSIVVWVPEGQTEPFSYDHDRNLILTDWYHKSSNEH 180

Query: 181 AAGLATPGSGFTWVGEPDSLLIQGRGRFNCSVLGSSSGTCNSSSTECVLYPLTVVPGKTY 240
           AAGLATPGSGFTWVGEPDSLLIQGRGRFNCSVLGSSSGTCNSSSTECVLYPLTVVPGKTY
Sbjct: 181 AAGLATPGSGFTWVGEPDSLLIQGRGRFNCSVLGSSSGTCNSSSTECVLYPLTVVPGKTY 240

Query: 241 RLRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQVNNLFIYSGETYSVLIKADQDPSR 300
           RLRVSSLTSLSALSFQIEGH+MTVVEADGHYVEPFQVNNLFIYSGETYSVLIKADQDPSR
Sbjct: 241 RLRVSSLTSLSALSFQIEGHSMTVVEADGHYVEPFQVNNLFIYSGETYSVLIKADQDPSR 300

Query: 301 NYWITTNVVSRSRTTPPGLAIFNYYPNHQSKLPPTTPPAPPAWNDVPPRLAQSQAIKARQ 360
           NYWITTNVVSRSRTTPPGLAIFNYYPNHQSK PPTTPPAPPAWNDVPPRLAQSQAIKARQ
Sbjct: 301 NYWITTNVVSRSRTTPPGLAIFNYYPNHQSKRPPTTPPAPPAWNDVPPRLAQSQAIKARQ 360

Query: 361 GFIHAPPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKHNLLHTFSQEKP 420
           GFIHAPPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKHNLLHTFSQEKP
Sbjct: 361 GFIHAPPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKHNLLHTFSQEKP 420

Query: 421 PTGYDFKNYDIFNPAPNPEATVSDAIFRLDFNSTVDIILQNANMMSPNNSETHPWHLHGH 480
           PTGYDFKNYDIFNPAPNPEATVSDAIFRLDFNSTVD+ILQNANMMSPNNSETHPWHLHGH
Sbjct: 421 PTGYDFKNYDIFNPAPNPEATVSDAIFRLDFNSTVDLILQNANMMSPNNSETHPWHLHGH 480

Query: 481 DFWVLGYGEGKFDIYNDPSKYNLENPIMKNTVPLHPYGWTALRFVADNPGVWAFHCHIDA 540
           DFWVLGYGEGKFDIYNDPSKYNLENPIMKNTVPLHPYGWTALRFVADNPGVWAFHCHIDA
Sbjct: 481 DFWVLGYGEGKFDIYNDPSKYNLENPIMKNTVPLHPYGWTALRFVADNPGVWAFHCHIDA 540

Query: 541 HFFMGMGVVFEEGIERVGKLPTSIMGCGESKRFLRP 577
           HFFMGMGVVFEEGIERVGKLPTSIMGCGESKRFLRP
Sbjct: 541 HFFMGMGVVFEEGIERVGKLPTSIMGCGESKRFLRP 576

[Close]
BLAST of PI0000263 vs. ExPASy TrEMBL
Match: A0A0A0KK62 (L-ascorbate oxidase OS=Cucumis sativus OX=3659 GN=Csa_6G500640 PE=3 SV=1)

HSP 1 Score: 1169.1 bits (3023), Expect = 0.0e+00
Identity = 554/576 (96.18%), Postives = 563/576 (97.74%), Query Frame = 0 

Query: 1   MASLLQSRQYLLILLSCFLFLLQSPTAESRTLNYKWEVKSELKSPDCFKKLTITINGRSP 60
           MAS LQS +YLLILLS   FLLQS TAESRTLNYKWEVKSELKSPDCFKK+T+TINGRSP
Sbjct: 1   MASPLQSTRYLLILLSFVFFLLQSQTAESRTLNYKWEVKSELKSPDCFKKVTLTINGRSP 60

Query: 61  GPTIYAHQGDTVIVELKNSLNTENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFRYQ 120
           GPTIYAHQGDTVIVELKNSL TENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFRYQ
Sbjct: 61  GPTIYAHQGDTVIVELKNSLFTENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFRYQ 120

Query: 121 FVVDRPGTYLYHAHYGMQREDGLYGSIVVWVPEGQTEPFSYDRDRNLILTDWYHKSSNEH 180
           FVVDRPGTYLYHAHYGMQREDGLYGSIVVWVPEGQTEPFSYD DRNLILTDWYHKSS+EH
Sbjct: 121 FVVDRPGTYLYHAHYGMQREDGLYGSIVVWVPEGQTEPFSYDLDRNLILTDWYHKSSHEH 180

Query: 181 AAGLATPGSGFTWVGEPDSLLIQGRGRFNCSVLGSSSGTCNSSSTECVLYPLTVVPGKTY 240
           A GLATPGSGF WVGEPDSLLIQGRGRFNCS+LGSSSGTCNSSSTECVL+PLTVVPGKTY
Sbjct: 181 ATGLATPGSGFNWVGEPDSLLIQGRGRFNCSILGSSSGTCNSSSTECVLHPLTVVPGKTY 240

Query: 241 RLRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQVNNLFIYSGETYSVLIKADQDPSR 300
           RLRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQVNNLFIYSGETYSVLIKADQDPSR
Sbjct: 241 RLRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQVNNLFIYSGETYSVLIKADQDPSR 300

Query: 301 NYWITTNVVSRSRTTPPGLAIFNYYPNHQSKLPPTTPPAPPAWNDVPPRLAQSQAIKARQ 360
           NYWITTNVVSRSRTTPPGLAIFNYYPNHQS+LPPTTPPAPPAWNDV PRLAQSQAIKARQ
Sbjct: 301 NYWITTNVVSRSRTTPPGLAIFNYYPNHQSRLPPTTPPAPPAWNDVTPRLAQSQAIKARQ 360

Query: 361 GFIHAPPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKHNLLHTFSQEKP 420
           GFIH PPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKHNLLHTFSQEKP
Sbjct: 361 GFIHTPPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKHNLLHTFSQEKP 420

Query: 421 PTGYDFKNYDIFNPAPNPEATVSDAIFRLDFNSTVDIILQNANMMSPNNSETHPWHLHGH 480
           PTGYDFKNYDIFNPAPNPEATVSDAI+RLDFNSTVDIILQNANMMSPNNSETHPWHLHGH
Sbjct: 421 PTGYDFKNYDIFNPAPNPEATVSDAIYRLDFNSTVDIILQNANMMSPNNSETHPWHLHGH 480

Query: 481 DFWVLGYGEGKFDIYNDPSKYNLENPIMKNTVPLHPYGWTALRFVADNPGVWAFHCHIDA 540
           DFWVLGYGEGKFD+Y DPSKYNLENPIMKNTVPLHPYGWTALRFVADNPGVWAFHCHIDA
Sbjct: 481 DFWVLGYGEGKFDLYKDPSKYNLENPIMKNTVPLHPYGWTALRFVADNPGVWAFHCHIDA 540

Query: 541 HFFMGMGVVFEEGIERVGKLPTSIMGCGESKRFLRP 577
           HFFMGMGVVFEEGIERVGKLPTSIMGCGESKRFLRP
Sbjct: 541 HFFMGMGVVFEEGIERVGKLPTSIMGCGESKRFLRP 576

[Close]
BLAST of PI0000263 vs. ExPASy TrEMBL
Match: A0A6J1KU77 (L-ascorbate oxidase OS=Cucurbita maxima OX=3661 GN=LOC111497239 PE=3 SV=1)

HSP 1 Score: 1088.2 bits (2813), Expect = 0.0e+00
Identity = 514/576 (89.24%), Postives = 542/576 (94.10%), Query Frame = 0 

Query: 1   MASLLQSRQYLLILLSCFLFLLQSPTAESRTLNYKWEVKSELKSPDCFKKLTITINGRSP 60
           M SLLQ RQYLLILL  F+FL +S TAESRTL Y+WEVKSELKSPDCFKKLT+TINGRSP
Sbjct: 1   MGSLLQRRQYLLILLCFFVFLWESRTAESRTLYYRWEVKSELKSPDCFKKLTLTINGRSP 60

Query: 61  GPTIYAHQGDTVIVELKNSLNTENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFRYQ 120
           GPTIYAHQGDTVIVELKNS  TENVAIHWHGIRQIGTPWSDGTEGVTQCP+LPGETF+YQ
Sbjct: 61  GPTIYAHQGDTVIVELKNSFVTENVAIHWHGIRQIGTPWSDGTEGVTQCPVLPGETFKYQ 120

Query: 121 FVVDRPGTYLYHAHYGMQREDGLYGSIVVWVPEGQTEPFSYDRDRNLILTDWYHKSSNEH 180
           FVVDRPGTYLYHAHYGMQREDGLYGSIVVW+PEGQTEPF+YD DRNLILTDWYHKSS+EH
Sbjct: 121 FVVDRPGTYLYHAHYGMQREDGLYGSIVVWLPEGQTEPFAYDHDRNLILTDWYHKSSHEH 180

Query: 181 AAGLATPGSGFTWVGEPDSLLIQGRGRFNCSVLGSSSGTCNSSSTECVLYPLTVVPGKTY 240
           +AGLA PGS F WVGEPDSLLIQGRGRFNCS+   +S TCNSSS +CVLYP+TVVPG+TY
Sbjct: 181 SAGLAAPGSAFNWVGEPDSLLIQGRGRFNCSL--GASATCNSSSPDCVLYPITVVPGRTY 240

Query: 241 RLRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQVNNLFIYSGETYSVLIKADQDPSR 300
           RLRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQV+NLFIYSGETYSVLIKADQ+PSR
Sbjct: 241 RLRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQVSNLFIYSGETYSVLIKADQNPSR 300

Query: 301 NYWITTNVVSRSRTTPPGLAIFNYYPNHQSKLPPTTPPAPPAWNDVPPRLAQSQAIKARQ 360
           NYWITTNVVSR+ TTP GLAIFNYYPNHQ K PPTTPPA PAWN V PRLAQS AIKARQ
Sbjct: 301 NYWITTNVVSRNSTTPLGLAIFNYYPNHQRKFPPTTPPAGPAWNAVAPRLAQSHAIKARQ 360

Query: 361 GFIHAPPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKHNLLHTFSQEKP 420
           GFIH PPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALK+NLLHTFSQ KP
Sbjct: 361 GFIHPPPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKNNLLHTFSQAKP 420

Query: 421 PTGYDFKNYDIFNPAPNPEATVSDAIFRLDFNSTVDIILQNANMMSPNNSETHPWHLHGH 480
           PTGYDFKNY+IF+P PNPEATVSDAI+RLDFNSTVDIILQNANMM+ N SETHPWHLHGH
Sbjct: 421 PTGYDFKNYNIFDPPPNPEATVSDAIYRLDFNSTVDIILQNANMMALNTSETHPWHLHGH 480

Query: 481 DFWVLGYGEGKFDIYNDPSKYNLENPIMKNTVPLHPYGWTALRFVADNPGVWAFHCHIDA 540
           DFWVLGYGEGKFD++ DPSKYNL NPIMKNTVPLHP+GWTALRFVADNPGVWAFHCHIDA
Sbjct: 481 DFWVLGYGEGKFDMFKDPSKYNLVNPIMKNTVPLHPFGWTALRFVADNPGVWAFHCHIDA 540

Query: 541 HFFMGMGVVFEEGIERVGKLPTSIMGCGESKRFLRP 577
           HFFMGMGVVFEEGIE+V KLPTSIMGCGESKRF+RP
Sbjct: 541 HFFMGMGVVFEEGIEKVQKLPTSIMGCGESKRFIRP 574

[Close]
BLAST of PI0000263 vs. ExPASy TrEMBL
Match: A0A6J1HE32 (L-ascorbate oxidase OS=Cucurbita moschata OX=3662 GN=LOC111463148 PE=3 SV=1)

HSP 1 Score: 1086.6 bits (2809), Expect = 0.0e+00
Identity = 516/576 (89.58%), Postives = 543/576 (94.27%), Query Frame = 0 

Query: 1   MASLLQSRQYLLILLSCFLFLLQSPTAESRTLNYKWEVKSELKSPDCFKKLTITINGRSP 60
           M SLLQSRQ LLILL  F+FL +S TAESRTL YKWEVKSELKSPDCFKKLT+TINGRSP
Sbjct: 1   MGSLLQSRQNLLILLCFFVFLRESRTAESRTLYYKWEVKSELKSPDCFKKLTLTINGRSP 60

Query: 61  GPTIYAHQGDTVIVELKNSLNTENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFRYQ 120
           GPTIYAHQGDTVIVELKNS  TENVAIHWHGIRQIGTPWSDGTEGVTQCP+LPGETF+YQ
Sbjct: 61  GPTIYAHQGDTVIVELKNSFVTENVAIHWHGIRQIGTPWSDGTEGVTQCPVLPGETFKYQ 120

Query: 121 FVVDRPGTYLYHAHYGMQREDGLYGSIVVWVPEGQTEPFSYDRDRNLILTDWYHKSSNEH 180
           FVVDRPGTYLYHAHYGMQREDGL+GSIVVW+PEGQTEPF+YD DRNLILTDWYHKSS+EH
Sbjct: 121 FVVDRPGTYLYHAHYGMQREDGLFGSIVVWLPEGQTEPFAYDHDRNLILTDWYHKSSHEH 180

Query: 181 AAGLATPGSGFTWVGEPDSLLIQGRGRFNCSVLGSSSGTCNSSSTECVLYPLTVVPGKTY 240
           +AGLA  GS FTWVGEPDSLLIQGRGRFNCS LG+S+ TCNSSS +CVLYP+TVVPG+TY
Sbjct: 181 SAGLAAAGSAFTWVGEPDSLLIQGRGRFNCSRLGASA-TCNSSSPDCVLYPITVVPGRTY 240

Query: 241 RLRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQVNNLFIYSGETYSVLIKADQDPSR 300
           RLRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQV+NLFIYSGETYSVLIKADQ+PSR
Sbjct: 241 RLRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQVSNLFIYSGETYSVLIKADQNPSR 300

Query: 301 NYWITTNVVSRSRTTPPGLAIFNYYPNHQSKLPPTTPPAPPAWNDVPPRLAQSQAIKARQ 360
           NYWITTNVVSR+ TTP GLAIFNYYPNHQ K PPTTPPA PAWN V PRLAQS AIKARQ
Sbjct: 301 NYWITTNVVSRNSTTPLGLAIFNYYPNHQRKFPPTTPPAGPAWNAVAPRLAQSHAIKARQ 360

Query: 361 GFIHAPPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKHNLLHTFSQEKP 420
           GFIH PPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALK+NLLHTFSQ KP
Sbjct: 361 GFIHPPPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKNNLLHTFSQAKP 420

Query: 421 PTGYDFKNYDIFNPAPNPEATVSDAIFRLDFNSTVDIILQNANMMSPNNSETHPWHLHGH 480
           PTGYDFKNY+IF P PNPEATVSDAI+RLDFNSTVDIILQNANMM+ N SETHPWHLHGH
Sbjct: 421 PTGYDFKNYNIFVPPPNPEATVSDAIYRLDFNSTVDIILQNANMMALNTSETHPWHLHGH 480

Query: 481 DFWVLGYGEGKFDIYNDPSKYNLENPIMKNTVPLHPYGWTALRFVADNPGVWAFHCHIDA 540
           DFWVLGYGEGKFD++ DPSKYNL NPIMKNTVPLHP+GWTALRFVADNPGVWAFHCHIDA
Sbjct: 481 DFWVLGYGEGKFDMFKDPSKYNLVNPIMKNTVPLHPFGWTALRFVADNPGVWAFHCHIDA 540

Query: 541 HFFMGMGVVFEEGIERVGKLPTSIMGCGESKRFLRP 577
           HFFMGMGVVFEEGIE+V KLPTSIMGCGESKRF+RP
Sbjct: 541 HFFMGMGVVFEEGIEKVQKLPTSIMGCGESKRFIRP 575

[Close]
BLAST of PI0000263 vs. NCBI nr
Match: XP_008440636.1 (PREDICTED: L-ascorbate oxidase [Cucumis melo] >KAA0036280.1 L-ascorbate oxidase [Cucumis melo var. makuwa] >TYK12674.1 L-ascorbate oxidase [Cucumis melo var. makuwa])

HSP 1 Score: 1192.2 bits (3083), Expect = 0.0e+00
Identity = 565/576 (98.09%), Postives = 570/576 (98.96%), Query Frame = 0 

Query: 1   MASLLQSRQYLLILLSCFLFLLQSPTAESRTLNYKWEVKSELKSPDCFKKLTITINGRSP 60
           MASLLQ +QY L+LLS FLFLLQS TAESRTLNYKWEVKSELKSPDCFKKLTITINGRSP
Sbjct: 1   MASLLQRKQYFLLLLSSFLFLLQSQTAESRTLNYKWEVKSELKSPDCFKKLTITINGRSP 60

Query: 61  GPTIYAHQGDTVIVELKNSLNTENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFRYQ 120
           GPTIYAHQGDTVIVELKNSL+TENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFRYQ
Sbjct: 61  GPTIYAHQGDTVIVELKNSLHTENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFRYQ 120

Query: 121 FVVDRPGTYLYHAHYGMQREDGLYGSIVVWVPEGQTEPFSYDRDRNLILTDWYHKSSNEH 180
           FVVDRPGTYLYHAHYGMQREDGLYGSIVVWVPEGQTEPFSYD DRNLILTDWYHKSSNEH
Sbjct: 121 FVVDRPGTYLYHAHYGMQREDGLYGSIVVWVPEGQTEPFSYDHDRNLILTDWYHKSSNEH 180

Query: 181 AAGLATPGSGFTWVGEPDSLLIQGRGRFNCSVLGSSSGTCNSSSTECVLYPLTVVPGKTY 240
           AAGLATPGSGFTWVGEPDSLLIQGRGRFNCSVLGSSSGTCNSSSTECVLYPLTVVPGKTY
Sbjct: 181 AAGLATPGSGFTWVGEPDSLLIQGRGRFNCSVLGSSSGTCNSSSTECVLYPLTVVPGKTY 240

Query: 241 RLRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQVNNLFIYSGETYSVLIKADQDPSR 300
           RLRVSSLTSLSALSFQIEGH+MTVVEADGHYVEPFQVNNLFIYSGETYSVLIKADQDPSR
Sbjct: 241 RLRVSSLTSLSALSFQIEGHSMTVVEADGHYVEPFQVNNLFIYSGETYSVLIKADQDPSR 300

Query: 301 NYWITTNVVSRSRTTPPGLAIFNYYPNHQSKLPPTTPPAPPAWNDVPPRLAQSQAIKARQ 360
           NYWITTNVVSRSRTTPPGLAIFNYYPNHQSK PPTTPPAPPAWNDVPPRLAQSQAIKARQ
Sbjct: 301 NYWITTNVVSRSRTTPPGLAIFNYYPNHQSKRPPTTPPAPPAWNDVPPRLAQSQAIKARQ 360

Query: 361 GFIHAPPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKHNLLHTFSQEKP 420
           GFIHAPPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKHNLLHTFSQEKP
Sbjct: 361 GFIHAPPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKHNLLHTFSQEKP 420

Query: 421 PTGYDFKNYDIFNPAPNPEATVSDAIFRLDFNSTVDIILQNANMMSPNNSETHPWHLHGH 480
           PTGYDFKNYDIFNPAPNPEATVSDAIFRLDFNSTVD+ILQNANMMSPNNSETHPWHLHGH
Sbjct: 421 PTGYDFKNYDIFNPAPNPEATVSDAIFRLDFNSTVDLILQNANMMSPNNSETHPWHLHGH 480

Query: 481 DFWVLGYGEGKFDIYNDPSKYNLENPIMKNTVPLHPYGWTALRFVADNPGVWAFHCHIDA 540
           DFWVLGYGEGKFDIYNDPSKYNLENPIMKNTVPLHPYGWTALRFVADNPGVWAFHCHIDA
Sbjct: 481 DFWVLGYGEGKFDIYNDPSKYNLENPIMKNTVPLHPYGWTALRFVADNPGVWAFHCHIDA 540

Query: 541 HFFMGMGVVFEEGIERVGKLPTSIMGCGESKRFLRP 577
           HFFMGMGVVFEEGIERVGKLPTSIMGCGESKRFLRP
Sbjct: 541 HFFMGMGVVFEEGIERVGKLPTSIMGCGESKRFLRP 576

[Close]
BLAST of PI0000263 vs. NCBI nr
Match: XP_004143482.1 (L-ascorbate oxidase [Cucumis sativus] >KGN48772.1 hypothetical protein Csa_003073 [Cucumis sativus])

HSP 1 Score: 1169.1 bits (3023), Expect = 0.0e+00
Identity = 554/576 (96.18%), Postives = 563/576 (97.74%), Query Frame = 0 

Query: 1   MASLLQSRQYLLILLSCFLFLLQSPTAESRTLNYKWEVKSELKSPDCFKKLTITINGRSP 60
           MAS LQS +YLLILLS   FLLQS TAESRTLNYKWEVKSELKSPDCFKK+T+TINGRSP
Sbjct: 1   MASPLQSTRYLLILLSFVFFLLQSQTAESRTLNYKWEVKSELKSPDCFKKVTLTINGRSP 60

Query: 61  GPTIYAHQGDTVIVELKNSLNTENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFRYQ 120
           GPTIYAHQGDTVIVELKNSL TENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFRYQ
Sbjct: 61  GPTIYAHQGDTVIVELKNSLFTENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFRYQ 120

Query: 121 FVVDRPGTYLYHAHYGMQREDGLYGSIVVWVPEGQTEPFSYDRDRNLILTDWYHKSSNEH 180
           FVVDRPGTYLYHAHYGMQREDGLYGSIVVWVPEGQTEPFSYD DRNLILTDWYHKSS+EH
Sbjct: 121 FVVDRPGTYLYHAHYGMQREDGLYGSIVVWVPEGQTEPFSYDLDRNLILTDWYHKSSHEH 180

Query: 181 AAGLATPGSGFTWVGEPDSLLIQGRGRFNCSVLGSSSGTCNSSSTECVLYPLTVVPGKTY 240
           A GLATPGSGF WVGEPDSLLIQGRGRFNCS+LGSSSGTCNSSSTECVL+PLTVVPGKTY
Sbjct: 181 ATGLATPGSGFNWVGEPDSLLIQGRGRFNCSILGSSSGTCNSSSTECVLHPLTVVPGKTY 240

Query: 241 RLRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQVNNLFIYSGETYSVLIKADQDPSR 300
           RLRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQVNNLFIYSGETYSVLIKADQDPSR
Sbjct: 241 RLRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQVNNLFIYSGETYSVLIKADQDPSR 300

Query: 301 NYWITTNVVSRSRTTPPGLAIFNYYPNHQSKLPPTTPPAPPAWNDVPPRLAQSQAIKARQ 360
           NYWITTNVVSRSRTTPPGLAIFNYYPNHQS+LPPTTPPAPPAWNDV PRLAQSQAIKARQ
Sbjct: 301 NYWITTNVVSRSRTTPPGLAIFNYYPNHQSRLPPTTPPAPPAWNDVTPRLAQSQAIKARQ 360

Query: 361 GFIHAPPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKHNLLHTFSQEKP 420
           GFIH PPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKHNLLHTFSQEKP
Sbjct: 361 GFIHTPPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKHNLLHTFSQEKP 420

Query: 421 PTGYDFKNYDIFNPAPNPEATVSDAIFRLDFNSTVDIILQNANMMSPNNSETHPWHLHGH 480
           PTGYDFKNYDIFNPAPNPEATVSDAI+RLDFNSTVDIILQNANMMSPNNSETHPWHLHGH
Sbjct: 421 PTGYDFKNYDIFNPAPNPEATVSDAIYRLDFNSTVDIILQNANMMSPNNSETHPWHLHGH 480

Query: 481 DFWVLGYGEGKFDIYNDPSKYNLENPIMKNTVPLHPYGWTALRFVADNPGVWAFHCHIDA 540
           DFWVLGYGEGKFD+Y DPSKYNLENPIMKNTVPLHPYGWTALRFVADNPGVWAFHCHIDA
Sbjct: 481 DFWVLGYGEGKFDLYKDPSKYNLENPIMKNTVPLHPYGWTALRFVADNPGVWAFHCHIDA 540

Query: 541 HFFMGMGVVFEEGIERVGKLPTSIMGCGESKRFLRP 577
           HFFMGMGVVFEEGIERVGKLPTSIMGCGESKRFLRP
Sbjct: 541 HFFMGMGVVFEEGIERVGKLPTSIMGCGESKRFLRP 576

[Close]
BLAST of PI0000263 vs. NCBI nr
Match: XP_038881120.1 (L-ascorbate oxidase [Benincasa hispida])

HSP 1 Score: 1156.7 bits (2991), Expect = 0.0e+00
Identity = 548/577 (94.97%), Postives = 561/577 (97.23%), Query Frame = 0 

Query: 1   MASLLQSRQYLLILLSCFLFLLQSPTAESRTLNYKWEVKSELKSPDCFKKLTITINGRSP 60
           MASLLQSRQYLLILL   LFL+QSP AESRTLNYKWEVKSELKSPDCFKKLT+TINGRSP
Sbjct: 18  MASLLQSRQYLLILLCFLLFLIQSPIAESRTLNYKWEVKSELKSPDCFKKLTMTINGRSP 77

Query: 61  GPTIYAHQGDTVIVELKNSLNTENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFRYQ 120
           GPTIYAHQGDTVIVELKNS +TENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETF+YQ
Sbjct: 78  GPTIYAHQGDTVIVELKNSFSTENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFKYQ 137

Query: 121 FVVDRPGTYLYHAHYGMQREDGLYGSIVVWVPEGQTEPFSYDRDRNLILTDWYHKSSNEH 180
           FVVDRPGTYLYHAHYGMQREDGLYGSIVVW+PEGQTEPFSYD DRNLILTDWYHK+S+EH
Sbjct: 138 FVVDRPGTYLYHAHYGMQREDGLYGSIVVWLPEGQTEPFSYDYDRNLILTDWYHKTSHEH 197

Query: 181 AAGLATPGSGFTWVGEPDSLLIQGRGRFNCSVLGSS-SGTCNSSSTECVLYPLTVVPGKT 240
           AAGLATPGSGFTWVGEPDSLLIQGRGRFNCSVLGSS S TCNSS+ EC+LYP+TVVPGKT
Sbjct: 198 AAGLATPGSGFTWVGEPDSLLIQGRGRFNCSVLGSSTSATCNSSNPECILYPITVVPGKT 257

Query: 241 YRLRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQVNNLFIYSGETYSVLIKADQDPS 300
           YRLRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQVNNLFIYSGETYSVLIKADQDPS
Sbjct: 258 YRLRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQVNNLFIYSGETYSVLIKADQDPS 317

Query: 301 RNYWITTNVVSRSRTTPPGLAIFNYYPNHQSKLPPTTPPAPPAWNDVPPRLAQSQAIKAR 360
           RNYWITTNVVSRSRTTPPGLAIFNYYPNHQ KLPPTTPPAPPAWNDV PRLAQS AIKAR
Sbjct: 318 RNYWITTNVVSRSRTTPPGLAIFNYYPNHQRKLPPTTPPAPPAWNDVAPRLAQSHAIKAR 377

Query: 361 QGFIHAPPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKHNLLHTFSQEK 420
           QGFIHAPPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKHNLLH FSQ+K
Sbjct: 378 QGFIHAPPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKHNLLHAFSQDK 437

Query: 421 PPTGYDFKNYDIFNPAPNPEATVSDAIFRLDFNSTVDIILQNANMMSPNNSETHPWHLHG 480
           PPTGYDFKNYDIFNPAPNPEATVSDAIFRLDFNSTVDIILQNANMMSPNNSETHPWHLHG
Sbjct: 438 PPTGYDFKNYDIFNPAPNPEATVSDAIFRLDFNSTVDIILQNANMMSPNNSETHPWHLHG 497

Query: 481 HDFWVLGYGEGKFDIYNDPSKYNLENPIMKNTVPLHPYGWTALRFVADNPGVWAFHCHID 540
           HDFWVLGYGEGKFDIY DPSK+NLENPIMKNTVPLHPYGWTALRFVADNPGVWAFHCHID
Sbjct: 498 HDFWVLGYGEGKFDIYKDPSKFNLENPIMKNTVPLHPYGWTALRFVADNPGVWAFHCHID 557

Query: 541 AHFFMGMGVVFEEGIERVGKLPTSIMGCGESKRFLRP 577
           AHFFMGMGVVF EGI+RV KLPTSIMGCGESKRFLRP
Sbjct: 558 AHFFMGMGVVFAEGIDRVQKLPTSIMGCGESKRFLRP 594

[Close]
BLAST of PI0000263 vs. NCBI nr
Match: KAG6594863.1 (L-ascorbate oxidase, partial [Cucurbita argyrosperma subsp. sororia] >KAG7026826.1 L-ascorbate oxidase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1093.2 bits (2826), Expect = 0.0e+00
Identity = 518/576 (89.93%), Postives = 545/576 (94.62%), Query Frame = 0 

Query: 1   MASLLQSRQYLLILLSCFLFLLQSPTAESRTLNYKWEVKSELKSPDCFKKLTITINGRSP 60
           M SLLQSRQYLLILL  F+FL +S TAESRTL YKWEVKSELKSPDCFKKLT+TINGRSP
Sbjct: 1   MGSLLQSRQYLLILLCFFVFLWESRTAESRTLYYKWEVKSELKSPDCFKKLTLTINGRSP 60

Query: 61  GPTIYAHQGDTVIVELKNSLNTENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFRYQ 120
           GPTIYAHQGDTVIVELKNS  TENVAIHWHGIRQIGTPWSDGTEGVTQCP+LPGETF+YQ
Sbjct: 61  GPTIYAHQGDTVIVELKNSFVTENVAIHWHGIRQIGTPWSDGTEGVTQCPVLPGETFKYQ 120

Query: 121 FVVDRPGTYLYHAHYGMQREDGLYGSIVVWVPEGQTEPFSYDRDRNLILTDWYHKSSNEH 180
           FVVDRPGTYLYHAHYGMQREDGLYGSIVVW+PEGQTEPF+YD DRNLILTDWYHKSS+EH
Sbjct: 121 FVVDRPGTYLYHAHYGMQREDGLYGSIVVWLPEGQTEPFAYDHDRNLILTDWYHKSSHEH 180

Query: 181 AAGLATPGSGFTWVGEPDSLLIQGRGRFNCSVLGSSSGTCNSSSTECVLYPLTVVPGKTY 240
           +AGLA  GS FTWVGEPDSLLIQGRGRFNCS+LG+S+ TCNSSS +CVLYP+TVVPG+TY
Sbjct: 181 SAGLAAAGSAFTWVGEPDSLLIQGRGRFNCSLLGASA-TCNSSSPDCVLYPITVVPGRTY 240

Query: 241 RLRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQVNNLFIYSGETYSVLIKADQDPSR 300
           RLRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQV+NLFIYSGETYSVLIKADQ+PSR
Sbjct: 241 RLRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQVSNLFIYSGETYSVLIKADQNPSR 300

Query: 301 NYWITTNVVSRSRTTPPGLAIFNYYPNHQSKLPPTTPPAPPAWNDVPPRLAQSQAIKARQ 360
           NYWITTNVVSR+ TTP GLAIFNYYPNHQ K PPTTPPA PAWN V PRLAQS AIKARQ
Sbjct: 301 NYWITTNVVSRNSTTPLGLAIFNYYPNHQRKFPPTTPPAGPAWNAVAPRLAQSHAIKARQ 360

Query: 361 GFIHAPPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKHNLLHTFSQEKP 420
           GFIH PPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALK+NLLHTFSQ KP
Sbjct: 361 GFIHPPPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKNNLLHTFSQAKP 420

Query: 421 PTGYDFKNYDIFNPAPNPEATVSDAIFRLDFNSTVDIILQNANMMSPNNSETHPWHLHGH 480
           PTGYDFKNY+IF P PNPEATVSDAI+RLDFNSTVDIILQNANMM+ N SETHPWHLHGH
Sbjct: 421 PTGYDFKNYNIFVPPPNPEATVSDAIYRLDFNSTVDIILQNANMMALNTSETHPWHLHGH 480

Query: 481 DFWVLGYGEGKFDIYNDPSKYNLENPIMKNTVPLHPYGWTALRFVADNPGVWAFHCHIDA 540
           DFWVLGYGEGKFD++ DPSKYNL NPIMKNTVPLHP+GWTALRFVADNPGVWAFHCHIDA
Sbjct: 481 DFWVLGYGEGKFDMFKDPSKYNLVNPIMKNTVPLHPFGWTALRFVADNPGVWAFHCHIDA 540

Query: 541 HFFMGMGVVFEEGIERVGKLPTSIMGCGESKRFLRP 577
           HFFMGMGVVFEEGIE+V KLPTSIMGCGESKRF+RP
Sbjct: 541 HFFMGMGVVFEEGIEKVQKLPTSIMGCGESKRFIRP 575

[Close]
BLAST of PI0000263 vs. NCBI nr
Match: XP_023518520.1 (L-ascorbate oxidase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1088.9 bits (2815), Expect = 0.0e+00
Identity = 516/576 (89.58%), Postives = 544/576 (94.44%), Query Frame = 0 

Query: 1   MASLLQSRQYLLILLSCFLFLLQSPTAESRTLNYKWEVKSELKSPDCFKKLTITINGRSP 60
           M SLLQSRQYLLILL  F+FL +S TAESRTL YKWEVKSELKSPDCFKKLT+TINGRSP
Sbjct: 1   MGSLLQSRQYLLILLCFFVFLWESRTAESRTLYYKWEVKSELKSPDCFKKLTLTINGRSP 60

Query: 61  GPTIYAHQGDTVIVELKNSLNTENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFRYQ 120
           GPTIYAHQGDTVIVELKNS  TENVAIHWHGIRQIGTPWSDGTEGVTQCP+LPGETF+YQ
Sbjct: 61  GPTIYAHQGDTVIVELKNSFVTENVAIHWHGIRQIGTPWSDGTEGVTQCPVLPGETFKYQ 120

Query: 121 FVVDRPGTYLYHAHYGMQREDGLYGSIVVWVPEGQTEPFSYDRDRNLILTDWYHKSSNEH 180
           FVVDRPGTYLYHAHYGMQREDGLYGSIVVW+PEGQTEPF+YD DRNLILTDWYHKSS+EH
Sbjct: 121 FVVDRPGTYLYHAHYGMQREDGLYGSIVVWLPEGQTEPFAYDHDRNLILTDWYHKSSHEH 180

Query: 181 AAGLATPGSGFTWVGEPDSLLIQGRGRFNCSVLGSSSGTCNSSSTECVLYPLTVVPGKTY 240
           +AGLA  G+ FTWVGEPDSLLIQGRGRFNCS+LG+S+ TCNSSS +CVLYP+TVVPG+TY
Sbjct: 181 SAGLAAAGAAFTWVGEPDSLLIQGRGRFNCSLLGASA-TCNSSSPDCVLYPITVVPGRTY 240

Query: 241 RLRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQVNNLFIYSGETYSVLIKADQDPSR 300
           RLRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQV+NLFIYSGETYSVLIKADQ+PSR
Sbjct: 241 RLRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQVSNLFIYSGETYSVLIKADQNPSR 300

Query: 301 NYWITTNVVSRSRTTPPGLAIFNYYPNHQSKLPPTTPPAPPAWNDVPPRLAQSQAIKARQ 360
           NYWITTNVVSR+ TTP GLAIFNYYPNHQ K PPTTPPA PAWN V PRLAQS AIKARQ
Sbjct: 301 NYWITTNVVSRNSTTPLGLAIFNYYPNHQRKFPPTTPPAGPAWNAVAPRLAQSHAIKARQ 360

Query: 361 GFIHAPPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKHNLLHTFSQEKP 420
           GFIH PPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALK+NLLHTFSQ KP
Sbjct: 361 GFIHPPPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKNNLLHTFSQAKP 420

Query: 421 PTGYDFKNYDIFNPAPNPEATVSDAIFRLDFNSTVDIILQNANMMSPNNSETHPWHLHGH 480
           PTGYDFKNY+I  P PNPEATVSDAI+RLDFNSTVDIILQNANMM+ N SETHPWHLHGH
Sbjct: 421 PTGYDFKNYNISVPPPNPEATVSDAIYRLDFNSTVDIILQNANMMALNTSETHPWHLHGH 480

Query: 481 DFWVLGYGEGKFDIYNDPSKYNLENPIMKNTVPLHPYGWTALRFVADNPGVWAFHCHIDA 540
           DFWVLGYGEGKFD++ DPSKYNL NPIMKNTVPLHP+GWTALRFVADNPGVWAFHCHIDA
Sbjct: 481 DFWVLGYGEGKFDMFKDPSKYNLVNPIMKNTVPLHPFGWTALRFVADNPGVWAFHCHIDA 540

Query: 541 HFFMGMGVVFEEGIERVGKLPTSIMGCGESKRFLRP 577
           HFFMGMGVVFEEGIE+V KLPTSIMGCGESKRF+RP
Sbjct: 541 HFFMGMGVVFEEGIEKVQKLPTSIMGCGESKRFIRP 575

[Close]
BLAST of PI0000263 vs. TAIR 10
Match: AT4G39830.1 (Cupredoxin superfamily protein )

HSP 1 Score: 803.1 bits (2073), Expect = 1.4e-232
Identity = 382/569 (67.14%), Postives = 447/569 (78.56%), Query Frame = 0 

Query: 13  ILLSCFLFL-LQSPTAESRTLNYKWEVKSELKSPDCFKKLTITINGRSPGPTIYAHQGDT 72
           +++ CF+ L   S   + +   +KWEVK E KSPDCF+KL ITING+ PGPTI A QGDT
Sbjct: 16  LMVLCFIALFFSSVLCQGKIRRFKWEVKYEFKSPDCFEKLVITINGKFPGPTIKAQQGDT 75

Query: 73  VIVELKNSLNTENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFRYQFVVDRPGTYLY 132
           ++VELKNS  TENVA+HWHGIRQIGTPW DG EGVTQCPILPGE F YQFVVDRPGTY+Y
Sbjct: 76  IVVELKNSFMTENVAVHWHGIRQIGTPWFDGVEGVTQCPILPGEVFIYQFVVDRPGTYMY 135

Query: 133 HAHYGMQREDGLYGSIVVWVPEGQTEPFSYDRDRNLILTDWYHKSSNEHAAGLATPGSGF 192
           H+HYGMQRE GL G I V  P  + EPF+YD DRN +LTDWYHKS +E A GLA+    F
Sbjct: 136 HSHYGMQRESGLIGMIQVSPPATEPEPFTYDYDRNFLLTDWYHKSMSEKATGLAS--IPF 195

Query: 193 TWVGEPDSLLIQGRGRFNCSVLGSS-----SGTCNSSSTECVLYPLTVVPGKTYRLRVSS 252
            WVGEP SL+IQGRGRFNCS   ++     SG CN S+ +C  + LTV+PGKTYRLR+ S
Sbjct: 196 KWVGEPQSLMIQGRGRFNCSNNLTTPPSLVSGVCNVSNADCSRFILTVIPGKTYRLRIGS 255

Query: 253 LTSLSALSFQIEGHTMTVVEADGHYVEPFQVNNLFIYSGETYSVLIKADQDPSRNYWITT 312
           LT+LSALSFQIEGH +TVVEADGHYVEPF V NLF+YSGETYSVL+KADQ+P RNYWIT+
Sbjct: 256 LTALSALSFQIEGHNLTVVEADGHYVEPFTVKNLFVYSGETYSVLLKADQNPRRNYWITS 315

Query: 313 NVVSRSRTTPPGLAIFNYYPNHQSKLPPTTPPA--PPAWNDVPPRLAQSQAIKARQGFIH 372
           ++VSR  TTPP  A+ NYYPNH  + PPT+  +   P WND   RLAQS AIKAR+GFIH
Sbjct: 316 SIVSRPATTPPATAVLNYYPNHPRRRPPTSESSNIVPEWNDTRSRLAQSLAIKARRGFIH 375

Query: 373 APPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKHNLLHTFS-QEKPPTG 432
           A P+ +DKVIVLLNTQN VNG RRWS+NNVS++ P TPYLIALK NL + F  +   P  
Sbjct: 376 ALPENSDKVIVLLNTQNEVNGYRRWSVNNVSYHHPKTPYLIALKQNLTNAFDWRFTAPEN 435

Query: 433 YDFKNYDIFNPAPNPEATVSDAIFRLDFNSTVDIILQNANMMSPNNSETHPWHLHGHDFW 492
           YD +NYDIF    N  AT SD I+RL FNSTVD+ILQNAN M+ NNSETHPWHLHGHDFW
Sbjct: 436 YDSRNYDIFAKPLNANATTSDGIYRLRFNSTVDVILQNANTMNANNSETHPWHLHGHDFW 495

Query: 493 VLGYGEGKFDIYNDPSKYNLENPIMKNTVPLHPYGWTALRFVADNPGVWAFHCHIDAHFF 552
           VLGYGEGKF+   DP +YN  +PI KNTV + P+GWTALRF ADNPGVW+FHCHI++HFF
Sbjct: 496 VLGYGEGKFNESEDPKRYNRVDPIKKNTVAVQPFGWTALRFRADNPGVWSFHCHIESHFF 555

Query: 553 MGMGVVFEEGIERVGKLPTSIMGCGESKR 573
           MGMG+VFE GI++V  LP+SIMGCG++KR
Sbjct: 556 MGMGIVFESGIDKVSSLPSSIMGCGQTKR 582

[Close]
BLAST of PI0000263 vs. TAIR 10
Match: AT5G21105.1 (Plant L-ascorbate oxidase )

HSP 1 Score: 589.0 bits (1517), Expect = 4.2e-168
Identity = 277/555 (49.91%), Postives = 367/555 (66.13%), Query Frame = 0 

Query: 26  TAESRTLNYKWEVKSELKSPDCFKKLTITINGRSPGPTIYAHQGDTVIVELKNSLNTENV 85
           TA +    Y WEV+ +  SPDC +   +T+NG  PGPTI A  GDT++V L N L TE +
Sbjct: 32  TASAAVREYHWEVEYKYWSPDCKEGAVMTVNGEFPGPTIKAFAGDTIVVNLTNKLTTEGL 91

Query: 86  AIHWHGIRQIGTPWSDGTEGVTQCPILPGETFRYQFVVDRPGTYLYHAHYGMQREDGLYG 145
            IHWHGIRQ G+PW+DG  GVTQC I PGETF Y F V++PGT+ YH HYGMQR  GLYG
Sbjct: 92  VIHWHGIRQFGSPWADGAAGVTQCAINPGETFTYNFTVEKPGTHFYHGHYGMQRSAGLYG 151

Query: 146 SIVVWVPEGQTEPFSYDRDRNLILTDWYHKSSNEHAAGLATPGSGFTWVGEPDSLLIQGR 205
           S++V V +G++E   YD + NL+L+DW+H++      GL++      W+GE  S+LI GR
Sbjct: 152 SLIVDVAKGKSERLRYDGEFNLLLSDWWHEAIPSQELGLSS--KPMRWIGEAQSILINGR 211

Query: 206 GRFNCSVLGSSSGTCN------SSSTECVLYPLTVVPGKTYRLRVSSLTSLSALSFQIEG 265
           G+FNCS+    S   +          +C    L V P KTYR+R+SS T+L++L+  ++G
Sbjct: 212 GQFNCSLAAQFSNNTSLPMCTFKEGDQCAPQILHVEPNKTYRIRLSSTTALASLNLAVQG 271

Query: 266 HTMTVVEADGHYVEPFQVNNLFIYSGETYSVLIKADQDPSRNYWITTNVVSRSRTTPPGL 325
           H + VVEADG+Y+ PF  +++ IYSGE+YSVL+  DQDPS+NY+I+  V  R   T   L
Sbjct: 272 HKLVVVEADGNYITPFTTDDIDIYSGESYSVLLTTDQDPSQNYYISVGVRGRKPNTTQAL 331

Query: 326 AIFNYYPNHQSKLPPTTPPAPPAWNDVPPRLAQSQAIKARQGFIHAPPKAADKVIVLLNT 385
            I NY     SKLP + PP  P W+D       S+ I +  G   +PPK   K ++LLNT
Sbjct: 332 TILNYVTAPASKLPSSPPPVTPRWDDFERSKNFSKKIFSAMG-SPSPPKKYRKRLILLNT 391

Query: 386 QNTVNGRRRWSLNNVSFNMPHTPYLIALKHNLLHTFSQEKPPTGYDFKNYDIFNPAPNPE 445
           QN ++G  +W++NNVS   P TPYL ++K+NL   F+++ PP  Y   +YDI NP P P 
Sbjct: 392 QNLIDGYTKWAINNVSLVTPATPYLGSVKYNLKLGFNRKSPPRSYRM-DYDIMNPPPFPN 451

Query: 446 ATVSDAIFRLDFNSTVDIILQNANMMSPNNSETHPWHLHGHDFWVLGYGEGKFDIYNDPS 505
            T  + I+   FN TVD+I+QNAN++    SE HPWHLHGHDFWVLGYG+GKF    D  
Sbjct: 452 TTTGNGIYVFPFNVTVDVIIQNANVLKGIVSEIHPWHLHGHDFWVLGYGDGKFKPGIDEK 511

Query: 506 KYNLENPIMKNTVPLHPYGWTALRFVADNPGVWAFHCHIDAHFFMGMGVVFEEGIERVGK 565
            YNL+NP ++NT  L+PYGWTA+RFV DNPGVW FHCHI+ H  MGMGVVF EG+ R+GK
Sbjct: 512 TYNLKNPPLRNTAILYPYGWTAIRFVTDNPGVWFFHCHIEPHLHMGMGVVFAEGLNRIGK 571

Query: 566 LPTSIMGCGESKRFL 575
           +P   +GCG +K+FL
Sbjct: 572 VPDEALGCGLTKQFL 582

[Close]
BLAST of PI0000263 vs. TAIR 10
Match: AT5G21100.1 (Plant L-ascorbate oxidase )

HSP 1 Score: 565.8 bits (1457), Expect = 3.8e-161
Identity = 271/557 (48.65%), Postives = 361/557 (64.81%), Query Frame = 0 

Query: 26  TAESRTLNYKWEVKSELKSPDCFKKLTITINGRSPGPTIYAHQGDTVIVELKNSLNTENV 85
           +A +  +   WEV+ +   PDC + + + ING+ PGPTI A  GDTVI+ + N L+TE V
Sbjct: 18  SASAAVVESTWEVEYKYWWPDCKEGIVMAINGQFPGPTIDAVAGDTVIIHVVNKLSTEGV 77

Query: 86  AIHWHGIRQIGTPWSDGTEGVTQCPILPGETFRYQFVVDRPGTYLYHAHYGMQREDGLYG 145
            IHWHGIRQ GTPW+DG  GVTQCPI PGETF Y+F+VD+ GT+ YH HYGMQR  GLYG
Sbjct: 78  VIHWHGIRQKGTPWADGAAGVTQCPINPGETFTYKFIVDKAGTHFYHGHYGMQRSSGLYG 137

Query: 146 SIVVWVPEGQTEPFSYDRDRNLILTDWYHKSSNEHAAGLATPGSGFTWVGEPDSLLIQGR 205
            ++V  P+   E   YD + NL+L+DW+H+S   HA  LA       W+GEP SLLI GR
Sbjct: 138 MLIVRSPK---ERLIYDGEFNLLLSDWWHQSI--HAQELALSSRPMRWIGEPQSLLINGR 197

Query: 206 GRFNCSVL-----GSSSGTCN-SSSTECVLYPLTVVPGKTYRLRVSSLTSLSALSFQIEG 265
           G+FNCS       G     C    + +C    L V P + YRLR++S T+L++L+  ++G
Sbjct: 198 GQFNCSQAAYFNKGGEKDVCTFKENDQCAPQTLRVEPNRVYRLRIASTTALASLNLAVQG 257

Query: 266 HTMTVVEADGHYVEPFQVNNLFIYSGETYSVLIKADQDPSRNYWITTNVVSRSRTTPPGL 325
           H + VVEADG+YV PF VN++ +YSGETYSVL+K +  PS+ YWI+  V  R   TP  L
Sbjct: 258 HQLVVVEADGNYVAPFTVNDIDVYSGETYSVLLKTNALPSKKYWISVGVRGREPKTPQAL 317

Query: 326 AIFNYYPNHQSKLPPTTPPAPPAWNDVPPRLAQSQAIKARQGFIHAPPKAADKVIVLLNT 385
            + NY    +S+ P   PP  P WND     + S+ I A +G+   P K+ D+ ++LLNT
Sbjct: 318 TVINYVDATESR-PSHPPPVTPIWNDTDRSKSFSKKIFAAKGYPKPPEKSHDQ-LILLNT 377

Query: 386 QNTVNGRRRWSLNNVSFNMPHTPYLIALKHNLLHTFSQEKPPTGYDFKNYDIFNPAPNPE 445
           QN      +WS+NNVS ++P TPYL ++++ L   +  + P       NYDI  P PNP 
Sbjct: 378 QNLYEDYTKWSINNVSLSVPVTPYLGSIRYGLKSAYDLKSPAKKLIMDNYDIMKPPPNPN 437

Query: 446 ATVSDAIFRLDFNSTVDIILQNANMMSPNNSETHPWHLHGHDFWVLGYGEGKFDIYNDPS 505
            T    I+   F   VD+ILQNAN++    SE HPWH+HGHDFWVLGYGEGKF    D  
Sbjct: 438 TTKGSGIYNFAFGIVVDVILQNANVLKGVISEIHPWHIHGHDFWVLGYGEGKFKPGIDEK 497

Query: 506 KYNLENPIMKNTVPLHPYGWTALRFVADNPGVWAFHCHIDAHFFMGMGVVFEEGIERVGK 565
            +NL+NP ++NTV L+P+GWTA+RFV DNPGVW FHCHI+ H  MGMGVVF EG++R+GK
Sbjct: 498 TFNLKNPPLRNTVVLYPFGWTAIRFVTDNPGVWFFHCHIEPHLHMGMGVVFVEGVDRIGK 557

Query: 566 --LPTSIMGCGESKRFL 575
             +P   +GCG ++++L
Sbjct: 558 MEIPDEALGCGLTRKWL 567

[Close]
BLAST of PI0000263 vs. TAIR 10
Match: AT5G21105.3 (Plant L-ascorbate oxidase )

HSP 1 Score: 523.5 bits (1347), Expect = 2.2e-148
Identity = 250/511 (48.92%), Postives = 333/511 (65.17%), Query Frame = 0 

Query: 26  TAESRTLNYKWEVKSELKSPDCFKKLTITINGRSPGPTIYAHQGDTVIVELKNSLNTENV 85
           TA +    Y WEV+ +  SPDC +   +T+NG  PGPTI A  GDT++V L N L TE +
Sbjct: 32  TASAAVREYHWEVEYKYWSPDCKEGAVMTVNGEFPGPTIKAFAGDTIVVNLTNKLTTEGL 91

Query: 86  AIHWHGIRQIGTPWSDGTEGVTQCPILPGETFRYQFVVDRPGTYLYHAHYGMQREDGLYG 145
            IHWHGIRQ G+PW+DG  GVTQC I PGETF Y F V++PGT+ YH HYGMQR  GLYG
Sbjct: 92  VIHWHGIRQFGSPWADGAAGVTQCAINPGETFTYNFTVEKPGTHFYHGHYGMQRSAGLYG 151

Query: 146 SIVVWVPEGQTEPFSYDRDRNLILTDWYHKSSNEHAAGLATPGSGFTWVGEPDSLLIQGR 205
           S++V V +G++E   YD + NL+L+DW+H++      GL++      W+GE  S+LI GR
Sbjct: 152 SLIVDVAKGKSERLRYDGEFNLLLSDWWHEAIPSQELGLSS--KPMRWIGEAQSILINGR 211

Query: 206 GRFNCSVLGSSSGTCN------SSSTECVLYPLTVVPGKTYRLRVSSLTSLSALSFQIEG 265
           G+FNCS+    S   +          +C    L V P KTYR+R+SS T+L++L+  ++G
Sbjct: 212 GQFNCSLAAQFSNNTSLPMCTFKEGDQCAPQILHVEPNKTYRIRLSSTTALASLNLAVQG 271

Query: 266 HTMTVVEADGHYVEPFQVNNLFIYSGETYSVLIKADQDPSRNYWITTNVVSRSRTTPPGL 325
           H + VVEADG+Y+ PF  +++ IYSGE+YSVL+  DQDPS+NY+I+  V  R   T   L
Sbjct: 272 HKLVVVEADGNYITPFTTDDIDIYSGESYSVLLTTDQDPSQNYYISVGVRGRKPNTTQAL 331

Query: 326 AIFNYYPNHQSKLPPTTPPAPPAWNDVPPRLAQSQAIKARQGFIHAPPKAADKVIVLLNT 385
            I NY     SKLP + PP  P W+D       S+ I +  G   +PPK   K ++LLNT
Sbjct: 332 TILNYVTAPASKLPSSPPPVTPRWDDFERSKNFSKKIFSAMG-SPSPPKKYRKRLILLNT 391

Query: 386 QNTVNGRRRWSLNNVSFNMPHTPYLIALKHNLLHTFSQEKPPTGYDFKNYDIFNPAPNPE 445
           QN ++G  +W++NNVS   P TPYL ++K+NL   F+++ PP  Y   +YDI NP P P 
Sbjct: 392 QNLIDGYTKWAINNVSLVTPATPYLGSVKYNLKLGFNRKSPPRSYRM-DYDIMNPPPFPN 451

Query: 446 ATVSDAIFRLDFNSTVDIILQNANMMSPNNSETHPWHLHGHDFWVLGYGEGKFDIYNDPS 505
            T  + I+   FN TVD+I+QNAN++    SE HPWHLHGHDFWVLGYG+GKF    D  
Sbjct: 452 TTTGNGIYVFPFNVTVDVIIQNANVLKGIVSEIHPWHLHGHDFWVLGYGDGKFKPGIDEK 511

Query: 506 KYNLENPIMKNTVPLHPYGWTALRFVADNPG 531
            YNL+NP ++NT  L+PYGWTA+RFV DNPG
Sbjct: 512 TYNLKNPPLRNTAILYPYGWTAIRFVTDNPG 538

[Close]
BLAST of PI0000263 vs. TAIR 10
Match: AT5G21105.2 (Plant L-ascorbate oxidase )

HSP 1 Score: 389.0 bits (998), Expect = 6.4e-108
Identity = 189/382 (49.48%), Postives = 251/382 (65.71%), Query Frame = 0 

Query: 199 SLLIQGRGRFNCSVLGSSSGTCN------SSSTECVLYPLTVVPGKTYRLRVSSLTSLSA 258
           S+LI GRG+FNCS+    S   +          +C    L V P KTYR+R+SS T+L++
Sbjct: 12  SILINGRGQFNCSLAAQFSNNTSLPMCTFKEGDQCAPQILHVEPNKTYRIRLSSTTALAS 71

Query: 259 LSFQIEGHTMTVVEADGHYVEPFQVNNLFIYSGETYSVLIKADQDPSRNYWITTNVVSRS 318
           L+  ++GH + VVEADG+Y+ PF  +++ IYSGE+YSVL+  DQDPS+NY+I+  V  R 
Sbjct: 72  LNLAVQGHKLVVVEADGNYITPFTTDDIDIYSGESYSVLLTTDQDPSQNYYISVGVRGRK 131

Query: 319 RTTPPGLAIFNYYPNHQSKLPPTTPPAPPAWNDVPPRLAQSQAIKARQGFIHAPPKAADK 378
             T   L I NY     SKLP + PP  P W+D       S+ I +  G   +PPK   K
Sbjct: 132 PNTTQALTILNYVTAPASKLPSSPPPVTPRWDDFERSKNFSKKIFSAMG-SPSPPKKYRK 191

Query: 379 VIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKHNLLHTFSQEKPPTGYDFKNYDIF 438
            ++LLNTQN ++G  +W++NNVS   P TPYL ++K+NL   F+++ PP  Y   +YDI 
Sbjct: 192 RLILLNTQNLIDGYTKWAINNVSLVTPATPYLGSVKYNLKLGFNRKSPPRSYRM-DYDIM 251

Query: 439 NPAPNPEATVSDAIFRLDFNSTVDIILQNANMMSPNNSETHPWHLHGHDFWVLGYGEGKF 498
           NP P P  T  + I+   FN TVD+I+QNAN++    SE HPWHLHGHDFWVLGYG+GKF
Sbjct: 252 NPPPFPNTTTGNGIYVFPFNVTVDVIIQNANVLKGIVSEIHPWHLHGHDFWVLGYGDGKF 311

Query: 499 DIYNDPSKYNLENPIMKNTVPLHPYGWTALRFVADNPGVWAFHCHIDAHFFMGMGVVFEE 558
               D   YNL+NP ++NT  L+PYGWTA+RFV DNPGVW FHCHI+ H  MGMGVVF E
Sbjct: 312 KPGIDEKTYNLKNPPLRNTAILYPYGWTAIRFVTDNPGVWFFHCHIEPHLHMGMGVVFAE 371

Query: 559 GIERVGKLPTSIMGCGESKRFL 575
           G+ R+GK+P   +GCG +K+FL
Sbjct: 372 GLNRIGKVPDEALGCGLTKQFL 391

The following BLAST results are available for this feature:
BLAST of PI0000263 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
P141331.5e-17352.77L-ascorbate oxidase OS=Cucumis sativus OX=3659 PE=1 SV=1[more]
P370642.8e-17251.45L-ascorbate oxidase OS=Cucurbita pepo var. melopepo OX=3665 PE=1 SV=1[more]
P247928.1e-17251.06L-ascorbate oxidase OS=Cucurbita maxima OX=3661 GN=AAO PE=1 SV=2[more]
M4DUF28.9e-17151.08L-ascorbate oxidase OS=Brassica rapa subsp. pekinensis OX=51351 GN=AO PE=2 SV=1[more]
Q405881.6e-16450.17L-ascorbate oxidase OS=Nicotiana tabacum OX=4097 GN=AAO PE=2 SV=1[more]
BLAST of PI0000263 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A5A7SYW40.0e+0098.09L-ascorbate oxidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G... [more]
A0A1S3B1510.0e+0098.09L-ascorbate oxidase OS=Cucumis melo OX=3656 GN=LOC103484994 PE=3 SV=1[more]
A0A0A0KK620.0e+0096.18L-ascorbate oxidase OS=Cucumis sativus OX=3659 GN=Csa_6G500640 PE=3 SV=1[more]
A0A6J1KU770.0e+0089.24L-ascorbate oxidase OS=Cucurbita maxima OX=3661 GN=LOC111497239 PE=3 SV=1[more]
A0A6J1HE320.0e+0089.58L-ascorbate oxidase OS=Cucurbita moschata OX=3662 GN=LOC111463148 PE=3 SV=1[more]
BLAST of PI0000263 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_008440636.10.0e+0098.09PREDICTED: L-ascorbate oxidase [Cucumis melo] >KAA0036280.1 L-ascorbate oxidase ... [more]
XP_004143482.10.0e+0096.18L-ascorbate oxidase [Cucumis sativus] >KGN48772.1 hypothetical protein Csa_00307... [more]
XP_038881120.10.0e+0094.97L-ascorbate oxidase [Benincasa hispida][more]
KAG6594863.10.0e+0089.93L-ascorbate oxidase, partial [Cucurbita argyrosperma subsp. sororia] >KAG7026826... [more]
XP_023518520.10.0e+0089.58L-ascorbate oxidase [Cucurbita pepo subsp. pepo][more]
BLAST of PI0000263 vs. TAIR 10
Match NameE-valueIdentityDescription
AT4G39830.11.4e-23267.14Cupredoxin superfamily protein [more]
AT5G21105.14.2e-16849.91Plant L-ascorbate oxidase [more]
AT5G21100.13.8e-16148.65Plant L-ascorbate oxidase [more]
AT5G21105.32.2e-14848.92Plant L-ascorbate oxidase [more]
AT5G21105.26.4e-10849.48Plant L-ascorbate oxidase [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001117Multicopper oxidase, type 1PFAMPF00394Cu-oxidasecoord: 166..326
e-value: 7.4E-48
score: 162.7
IPR008972CupredoxinGENE3D2.60.40.420coord: 20..152
e-value: 1.3E-47
score: 163.8
IPR008972CupredoxinGENE3D2.60.40.420coord: 364..574
e-value: 1.6E-69
score: 235.8
IPR008972CupredoxinGENE3D2.60.40.420coord: 159..362
e-value: 1.5E-62
score: 213.3
IPR008972CupredoxinSUPERFAMILY49503Cupredoxinscoord: 19..178
IPR008972CupredoxinSUPERFAMILY49503Cupredoxinscoord: 367..569
IPR008972CupredoxinSUPERFAMILY49503Cupredoxinscoord: 158..347
IPR011706Multicopper oxidase, C-terminalPFAMPF07731Cu-oxidase_2coord: 430..553
e-value: 2.2E-37
score: 127.8
IPR017760L-ascorbate oxidase, plantsTIGRFAMTIGR03388TIGR03388coord: 33..569
e-value: 6.1E-281
score: 930.7
IPR011707Multicopper oxidase, N-termianlPFAMPF07732Cu-oxidase_3coord: 41..150
e-value: 9.5E-39
score: 132.1
IPR045087Multicopper oxidasePANTHERPTHR11709MULTI-COPPER OXIDASEcoord: 14..570
NoneNo IPR availablePANTHERPTHR11709:SF218OS09G0365900 PROTEINcoord: 14..570
IPR033138Multicopper oxidases, conserved sitePROSITEPS00079MULTICOPPER_OXIDASE1coord: 530..550
IPR002355Multicopper oxidase, copper-binding sitePROSITEPS00080MULTICOPPER_OXIDASE2coord: 535..546
IPR034267Ascorbate oxidase, third cupredoxin domainCDDcd13893CuRO_3_AAOcoord: 370..561
e-value: 1.2108E-83
score: 255.808

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0000263.1PI0000263.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:1900367 positive regulation of defense response to insect
cellular_component GO:0005576 extracellular region
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0005507 copper ion binding
molecular_function GO:0008447 L-ascorbate oxidase activity
molecular_function GO:0016491 oxidoreductase activity