Moc11g31760 (gene) Bitter gourd (OHB3-1) v2

BLAST of Moc11g31760 vs. NCBI nr
Match: XP_022155217.1 (hydrolase tropI [Momordica charantia])

HSP 1 Score: 606.7 bits (1563), Expect = 1.1e-169
Identity = 294/294 (100.00%), Postives = 294/294 (100.00%), Query Frame = 0

Query: 1   MGLASAGAAQMISLHIGTPFPSPLHLRKCNMGVWKWKVKREKKGGQVCSSHRLEGEIDEG 60
           MGLASAGAAQMISLHIGTPFPSPLHLRKCNMGVWKWKVKREKKGGQVCSSHRLEGEIDEG
Sbjct: 1   MGLASAGAAQMISLHIGTPFPSPLHLRKCNMGVWKWKVKREKKGGQVCSSHRLEGEIDEG 60

Query: 61  CELVSGMELSIGEQPDRTVPAHLFKAVKNNNGTAILLLSDLFAFQDSSITDFAYRVACNG 120
           CELVSGMELSIGEQPDRTVPAHLFKAVKNNNGTAILLLSDLFAFQDSSITDFAYRVACNG
Sbjct: 61  CELVSGMELSIGEQPDRTVPAHLFKAVKNNNGTAILLLSDLFAFQDSSITDFAYRVACNG 120

Query: 121 FNVLLPDVFHGDPWKKKQPVEQTAKQDMQRVAEDIGTATKWLVEEFSAAGISTKLGIMGF 180
           FNVLLPDVFHGDPWKKKQPVEQTAKQDMQRVAEDIGTATKWLVEEFSAAGISTKLGIMGF
Sbjct: 121 FNVLLPDVFHGDPWKKKQPVEQTAKQDMQRVAEDIGTATKWLVEEFSAAGISTKLGIMGF 180

Query: 181 GYGGGQVIDVLARDQGACFAIGASFYGTNIDPSLGSQVKVPVLLISGDNDPLCPVSVMKD 240
           GYGGGQVIDVLARDQGACFAIGASFYGTNIDPSLGSQVKVPVLLISGDNDPLCPVSVMKD
Sbjct: 181 GYGGGQVIDVLARDQGACFAIGASFYGTNIDPSLGSQVKVPVLLISGDNDPLCPVSVMKD 240

Query: 241 LEKGIGKGSKLVIFGGRGHGFFHKPGSPEEDEDAERAFLMLRNWLHDGLVVTNI 295
           LEKGIGKGSKLVIFGGRGHGFFHKPGSPEEDEDAERAFLMLRNWLHDGLVVTNI
Sbjct: 241 LEKGIGKGSKLVIFGGRGHGFFHKPGSPEEDEDAERAFLMLRNWLHDGLVVTNI 294

BLAST of Moc11g31760 vs. NCBI nr
Match: XP_038890567.1 (hydrolase tropI [Benincasa hispida])

HSP 1 Score: 442.2 bits (1136), Expect = 3.5e-120
Identity = 230/317 (72.56%), Postives = 254/317 (80.13%), Query Frame = 0

Query: 1   MGLASAGAAQMISL----------HIGTP-------FPSPLHLRKC-NMGVWKWKVKREK 60
           MGLASAG AQ+ISL           IGTP       F SPL  RKC N   WKWK KREK
Sbjct: 1   MGLASAG-AQLISLPPTGSSSCRRRIGTPFQLPYFRFTSPLPFRKCGNRSDWKWKGKREK 60

Query: 61  KGGQVCSSHRLEG-----EIDEGCELVSGMELSIGEQPDRTVPAHLFKAVKNNNGTAILL 120
           KGG+VC S  +EG     + D  CELV+GMELSIGEQP  ++PA+LFKAVKNNNGTAILL
Sbjct: 61  KGGKVCYS--VEGVEDDDDDDAACELVNGMELSIGEQPHHSIPAYLFKAVKNNNGTAILL 120

Query: 121 LSDLFAFQDSSITDFAYRVACNGFNVLLPDVFHGDPWKKKQPVEQTAKQDMQRVAEDIGT 180
           LSDLFAFQ SSI DFAYR+ACNGFNVLLPD+ HGD WKK     Q+ K+DMQR+AEDIGT
Sbjct: 121 LSDLFAFQHSSIRDFAYRLACNGFNVLLPDLIHGDSWKK-----QSTKRDMQRIAEDIGT 180

Query: 181 ATKWLVEEFSAAGISTKLGIMGFGYGGGQVIDVLARDQGACFAIGASFYGTNIDPSLGSQ 240
            TKWLV+EFSAAG+S KLGIMGFGYGGGQVI+VLARD+GACF IGAS YGT +DPSL ++
Sbjct: 181 VTKWLVDEFSAAGLSRKLGIMGFGYGGGQVIEVLARDEGACFCIGASLYGTEVDPSLATK 240

Query: 241 VKVPVLLISGDNDPLCPVSVMKDLEKGIGKGSKLVIFGGRGHGFFHKPGSPEEDEDAERA 295
           VKVPVLLISGDNDPLCPVSVMKDLEK +G+GSKLVIFGGRGH F H P SPEEDEDAE+A
Sbjct: 241 VKVPVLLISGDNDPLCPVSVMKDLEKRMGEGSKLVIFGGRGHDFVHNPRSPEEDEDAEQA 300

BLAST of Moc11g31760 vs. NCBI nr
Match: XP_004141569.1 (uncharacterized protein LOC101210518 [Cucumis sativus] >KGN52723.1 hypothetical protein Csa_008898 [Cucumis sativus])

HSP 1 Score: 417.2 bits (1071), Expect = 1.2e-112
Identity = 215/309 (69.58%), Postives = 240/309 (77.67%), Query Frame = 0

Query: 1   MGLASAGAAQMISL--------HIGTPFPS-------PLHLRK-CNMGVWKWKVKREKKG 60
           MG ASAG AQ+ISL         +G PF         PL  RK  NMGVW+WK K     
Sbjct: 1   MGFASAG-AQLISLPPTGSSSRRVGKPFQPPYFKFAYPLPSRKYSNMGVWRWKGKLRYS- 60

Query: 61  GQVCSSHRLEGEIDEGCELVSGMELSIGEQPDRTVPAHLFKAVKNNNGTAILLLSDLFAF 120
                    + + D+ CELV+GMELSIGEQP  ++PA+LFKAVKNNNGTAILLLSDLFAF
Sbjct: 61  ---VEGRMKDDDDDDACELVNGMELSIGEQPHHSIPAYLFKAVKNNNGTAILLLSDLFAF 120

Query: 121 QDSSITDFAYRVACNGFNVLLPDVFHGDPWKKKQPVEQTAKQDMQRVAEDIGTATKWLVE 180
           Q SSI DFAYR+AC GFNVLLPD+ H D W +K P E  AKQ+MQR+AEDIGT TKWL E
Sbjct: 121 QHSSIRDFAYRLACIGFNVLLPDLVHEDVW-EKPPAEHCAKQEMQRIAEDIGTVTKWLTE 180

Query: 181 EFSAAGISTKLGIMGFGYGGGQVIDVLARDQGACFAIGASFYGTNIDPSLGSQVKVPVLL 240
           EFSAAG+S KLGI+GFGYGGG+VIDVLARD+G CF IGAS YGT +DPSL ++VKVPVLL
Sbjct: 181 EFSAAGLSMKLGIIGFGYGGGRVIDVLARDEGTCFLIGASIYGTQVDPSLATKVKVPVLL 240

Query: 241 ISGDNDPLCPVSVMKDLEKGIGKGSKLVIFGGRGHGFFHKPGSPEEDEDAERAFLMLRNW 294
           ISGDNDPLCPVSVMKDLEKGIG+GSKLVIFGGRGHGF H PGSPEED+DAE+AFLMLRNW
Sbjct: 241 ISGDNDPLCPVSVMKDLEKGIGEGSKLVIFGGRGHGFVHNPGSPEEDDDAEKAFLMLRNW 300

BLAST of Moc11g31760 vs. NCBI nr
Match: XP_008459689.1 (PREDICTED: uncharacterized protein LOC103498731 [Cucumis melo] >KAA0039216.1 carboxymethylenebutenolidase-like protein [Cucumis melo var. makuwa] >TYK00404.1 carboxymethylenebutenolidase-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 408.7 bits (1049), Expect = 4.3e-110
Identity = 211/309 (68.28%), Postives = 243/309 (78.64%), Query Frame = 0

Query: 1   MGLASAGAAQMISL--------HIGTP-------FPSPLHLRKC-NMGVWKWKVKREKKG 60
           MG ASAG AQ+ISL         +  P       F  PL   K  N G WKWK K     
Sbjct: 1   MGFASAG-AQLISLPPTGSSSRRVEKPIQPPYSQFAYPLPSWKYNNWGDWKWKGKLR--- 60

Query: 61  GQVCSSHRLEGEIDEGCELVSGMELSIGEQPDRTVPAHLFKAVKNNNGTAILLLSDLFAF 120
                  R++ + D+ CELV+GMELSIGEQP  ++PA+LFKAVKNNNGTAILLLSDLFAF
Sbjct: 61  -YYSVKGRVKDDDDDACELVNGMELSIGEQPHHSIPAYLFKAVKNNNGTAILLLSDLFAF 120

Query: 121 QDSSITDFAYRVACNGFNVLLPDVFHGDPWKKKQPVEQTAKQDMQRVAEDIGTATKWLVE 180
           QDSSI DFAYR+ACNGFNVLLPD+ + DPW +K P + +AKQ+MQR+AEDIGT TKWL +
Sbjct: 121 QDSSIRDFAYRLACNGFNVLLPDLVNEDPW-EKPPAQHSAKQEMQRIAEDIGTVTKWLTD 180

Query: 181 EFSAAGISTKLGIMGFGYGGGQVIDVLARDQGACFAIGASFYGTNIDPSLGSQVKVPVLL 240
           EFSAAG+S KLGI+GFG+GGG+VIDVLARD+GACF IGAS YGT +DPSL ++VKVPVLL
Sbjct: 181 EFSAAGLSRKLGIIGFGHGGGRVIDVLARDKGACFLIGASLYGTEVDPSLATKVKVPVLL 240

Query: 241 ISGDNDPLCPVSVMKDLEKGIGKGSKLVIFGGRGHGFFHKPGSPEEDEDAERAFLMLRNW 294
           ISGD+DPLCPVSVMKDLEKG+G+GSKLVIF GRGHGF H PGSPEED+DAE+AFLMLRNW
Sbjct: 241 ISGDDDPLCPVSVMKDLEKGMGEGSKLVIFEGRGHGFVHNPGSPEEDDDAEQAFLMLRNW 300

BLAST of Moc11g31760 vs. NCBI nr
Match: XP_022973982.1 (carboxymethylenebutenolidase homolog [Cucurbita maxima])

HSP 1 Score: 398.3 bits (1022), Expect = 5.8e-107
Identity = 210/306 (68.63%), Postives = 242/306 (79.08%), Query Frame = 0

Query: 1   MGLASAGAAQMISL--------HIGTPF-PSPLHLRKCNMGVWKWKVKREKKGGQVC-SS 60
           MG ASAG AQ IS          +G PF PS       +  + + K KREKKGG+VC S+
Sbjct: 1   MGSASAG-AQWISAPPTGRSGRRMGIPFQPSFRCAFPISNNIGQLKGKREKKGGKVCYSA 60

Query: 61  HRLEGEID--EGCELVSGMELSIGEQPDRTVPAHLFKAVKNNNGTAILLLSDLFAFQDSS 120
             ++ EID  E CELVSGMELSIGE+   ++ AHLFKAVKNNNGTAILLL+DLF+FQDSS
Sbjct: 61  EGVKDEIDAEEACELVSGMELSIGEEAHHSITAHLFKAVKNNNGTAILLLTDLFSFQDSS 120

Query: 121 ITDFAYRVACNGFNVLLPDVFHGDPWKKKQPVEQTAKQDMQRVAEDIGTATKWLVEEFSA 180
           + DFAYR+ACNGFNVLLPDVFHGD  ++            Q+  EDIG ATKWLV+EF A
Sbjct: 121 VRDFAYRLACNGFNVLLPDVFHGDSCEE------------QKAVEDIGAATKWLVDEFLA 180

Query: 181 AGISTKLGIMGFGYGGGQVIDVLARDQGACFAIGASFYGTNIDPSLGSQVKVPVLLISGD 240
           AGIS KLGI+GFGYGGG+VIDVLA+DQG+ F+IGAS YGT ++PSL ++V+VPVLLISGD
Sbjct: 181 AGISRKLGIVGFGYGGGRVIDVLAQDQGSYFSIGASLYGTEVNPSLATKVEVPVLLISGD 240

Query: 241 NDPLCPVSVMKDLEKGIGKGSKLVIFGGRGHGFFHKPGSPEEDEDAERAFLMLRNWLHDG 295
           NDPLCPVSVMKDLEKG+G+GSKLVIFGGRGHGF HKPGSPEEDEDAE+AFLMLRNWLHDG
Sbjct: 241 NDPLCPVSVMKDLEKGMGEGSKLVIFGGRGHGFVHKPGSPEEDEDAEQAFLMLRNWLHDG 293

BLAST of Moc11g31760 vs. ExPASy Swiss-Prot
Match: B8M9K0 (Hydrolase tropI OS=Talaromyces stipitatus (strain ATCC 10500 / CBS 375.48 / QM 6759 / NRRL 1006) OX=441959 GN=tropI PE=3 SV=1)

HSP 1 Score: 50.8 bits (120), Expect = 3.0e-05
Identity = 40/179 (22.35%), Postives = 82/179 (45.81%), Query Frame = 0

Query: 95  ILLLSDLFAFQDSSITDFAYRVACNGFNVLLPDVFHGDPWKKKQPVE---------QTAK 154
           +L+L+D+   + S+    A + A NG+ V++PD+F GD     +P E             
Sbjct: 46  VLILTDIVGHRFSNAQIIADQFAENGYFVMMPDLFLGDAVPLNKPGEFDMGKWRSGAYHP 105

Query: 155 QDMQRVAEDIGTATKWLVEEFSAAGISTKLGIMGFGYGGGQVIDVLARDQGACFAIGASF 214
           Q    + E +    +  + E  +     K+G +G+ +GG  V+  L   +     +G + 
Sbjct: 106 QGKNHLPETVDPIVEVCLSEMRSKYQCKKIGAVGYCFGGKYVVRHLIPGK---MDVGYTA 165

Query: 215 YGTNIDPSLGSQVKVPVLLISGDNDPLCPVS---VMKDLEKGIGKGSKLVIFGGRGHGF 262
           + ++ID S    +K P+ + +   D + P     V +++ + +G   ++ ++ G  HGF
Sbjct: 166 HPSHIDESELKGIKGPLAIAAAAKDNIFPAEKRHVSEEILQEVGFPYQINLYSGVSHGF 221

BLAST of Moc11g31760 vs. ExPASy Swiss-Prot
Match: A0A2V5GSC6 (Hydrolase pyvD OS=Aspergillus violaceofuscus (strain CBS 115571) OX=1450538 GN=pyvD PE=3 SV=1)

HSP 1 Score: 45.8 bits (107), Expect = 9.6e-04
Identity = 48/194 (24.74%), Postives = 82/194 (42.27%), Query Frame = 0

Query: 79  VPAHLFKAVKNNNGTAILLLSDLFAFQDSSITDFAYRVACNGFNVLLPDVFHGDPWKKKQ 138
           +P ++      +   AIL LSD+F     +    A   A NG+ V++PD+F GDP     
Sbjct: 30  IPVYVSYPPDKSTHNAILFLSDIFGLALVNSKLIADLFAANGYLVVMPDLFQGDP----V 89

Query: 139 PVEQT--------AKQDMQRVAEDIGTATKWLVEEFSAAGISTKLGIMGFGYGGGQVIDV 198
           PV+ +         +  +    + I TAT   + E        ++G +G+ YGG  V+  
Sbjct: 90  PVDHSPSFQIMDWLQGHLPPQTDPIVTAT---LREMRERLGCQRIGGVGYCYGGKYVVRY 149

Query: 199 LARDQGACFAIGASFYGTNIDPSLGSQVKVPVLLISGDNDPLCPVSVMKDLEKGIGK--- 258
           L   Q     +G   + T I+P     ++ P+ + +   D L P     + E  + +   
Sbjct: 150 LHPGQ---LDVGFVAHPTFIEPDELKAIEGPLSISASAQDNLFPPEKRHESETILAQLDV 209

Query: 259 GSKLVIFGGRGHGF 262
             ++ IF    HGF
Sbjct: 210 PYQINIFSDVEHGF 213

BLAST of Moc11g31760 vs. ExPASy TrEMBL
Match: A0A6J1DNR6 (hydrolase tropI OS=Momordica charantia OX=3673 GN=LOC111022362 PE=4 SV=1)

HSP 1 Score: 606.7 bits (1563), Expect = 5.2e-170
Identity = 294/294 (100.00%), Postives = 294/294 (100.00%), Query Frame = 0

Query: 1   MGLASAGAAQMISLHIGTPFPSPLHLRKCNMGVWKWKVKREKKGGQVCSSHRLEGEIDEG 60
           MGLASAGAAQMISLHIGTPFPSPLHLRKCNMGVWKWKVKREKKGGQVCSSHRLEGEIDEG
Sbjct: 1   MGLASAGAAQMISLHIGTPFPSPLHLRKCNMGVWKWKVKREKKGGQVCSSHRLEGEIDEG 60

Query: 61  CELVSGMELSIGEQPDRTVPAHLFKAVKNNNGTAILLLSDLFAFQDSSITDFAYRVACNG 120
           CELVSGMELSIGEQPDRTVPAHLFKAVKNNNGTAILLLSDLFAFQDSSITDFAYRVACNG
Sbjct: 61  CELVSGMELSIGEQPDRTVPAHLFKAVKNNNGTAILLLSDLFAFQDSSITDFAYRVACNG 120

Query: 121 FNVLLPDVFHGDPWKKKQPVEQTAKQDMQRVAEDIGTATKWLVEEFSAAGISTKLGIMGF 180
           FNVLLPDVFHGDPWKKKQPVEQTAKQDMQRVAEDIGTATKWLVEEFSAAGISTKLGIMGF
Sbjct: 121 FNVLLPDVFHGDPWKKKQPVEQTAKQDMQRVAEDIGTATKWLVEEFSAAGISTKLGIMGF 180

Query: 181 GYGGGQVIDVLARDQGACFAIGASFYGTNIDPSLGSQVKVPVLLISGDNDPLCPVSVMKD 240
           GYGGGQVIDVLARDQGACFAIGASFYGTNIDPSLGSQVKVPVLLISGDNDPLCPVSVMKD
Sbjct: 181 GYGGGQVIDVLARDQGACFAIGASFYGTNIDPSLGSQVKVPVLLISGDNDPLCPVSVMKD 240

Query: 241 LEKGIGKGSKLVIFGGRGHGFFHKPGSPEEDEDAERAFLMLRNWLHDGLVVTNI 295
           LEKGIGKGSKLVIFGGRGHGFFHKPGSPEEDEDAERAFLMLRNWLHDGLVVTNI
Sbjct: 241 LEKGIGKGSKLVIFGGRGHGFFHKPGSPEEDEDAERAFLMLRNWLHDGLVVTNI 294

BLAST of Moc11g31760 vs. ExPASy TrEMBL
Match: A0A0A0KT04 (DLH domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G652280 PE=4 SV=1)

HSP 1 Score: 417.2 bits (1071), Expect = 5.9e-113
Identity = 215/309 (69.58%), Postives = 240/309 (77.67%), Query Frame = 0

Query: 1   MGLASAGAAQMISL--------HIGTPFPS-------PLHLRK-CNMGVWKWKVKREKKG 60
           MG ASAG AQ+ISL         +G PF         PL  RK  NMGVW+WK K     
Sbjct: 1   MGFASAG-AQLISLPPTGSSSRRVGKPFQPPYFKFAYPLPSRKYSNMGVWRWKGKLRYS- 60

Query: 61  GQVCSSHRLEGEIDEGCELVSGMELSIGEQPDRTVPAHLFKAVKNNNGTAILLLSDLFAF 120
                    + + D+ CELV+GMELSIGEQP  ++PA+LFKAVKNNNGTAILLLSDLFAF
Sbjct: 61  ---VEGRMKDDDDDDACELVNGMELSIGEQPHHSIPAYLFKAVKNNNGTAILLLSDLFAF 120

Query: 121 QDSSITDFAYRVACNGFNVLLPDVFHGDPWKKKQPVEQTAKQDMQRVAEDIGTATKWLVE 180
           Q SSI DFAYR+AC GFNVLLPD+ H D W +K P E  AKQ+MQR+AEDIGT TKWL E
Sbjct: 121 QHSSIRDFAYRLACIGFNVLLPDLVHEDVW-EKPPAEHCAKQEMQRIAEDIGTVTKWLTE 180

Query: 181 EFSAAGISTKLGIMGFGYGGGQVIDVLARDQGACFAIGASFYGTNIDPSLGSQVKVPVLL 240
           EFSAAG+S KLGI+GFGYGGG+VIDVLARD+G CF IGAS YGT +DPSL ++VKVPVLL
Sbjct: 181 EFSAAGLSMKLGIIGFGYGGGRVIDVLARDEGTCFLIGASIYGTQVDPSLATKVKVPVLL 240

Query: 241 ISGDNDPLCPVSVMKDLEKGIGKGSKLVIFGGRGHGFFHKPGSPEEDEDAERAFLMLRNW 294
           ISGDNDPLCPVSVMKDLEKGIG+GSKLVIFGGRGHGF H PGSPEED+DAE+AFLMLRNW
Sbjct: 241 ISGDNDPLCPVSVMKDLEKGIGEGSKLVIFGGRGHGFVHNPGSPEEDDDAEKAFLMLRNW 300

BLAST of Moc11g31760 vs. ExPASy TrEMBL
Match: A0A5A7TB03 (Carboxymethylenebutenolidase-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold169G00030 PE=4 SV=1)

HSP 1 Score: 408.7 bits (1049), Expect = 2.1e-110
Identity = 211/309 (68.28%), Postives = 243/309 (78.64%), Query Frame = 0

Query: 1   MGLASAGAAQMISL--------HIGTP-------FPSPLHLRKC-NMGVWKWKVKREKKG 60
           MG ASAG AQ+ISL         +  P       F  PL   K  N G WKWK K     
Sbjct: 1   MGFASAG-AQLISLPPTGSSSRRVEKPIQPPYSQFAYPLPSWKYNNWGDWKWKGKLR--- 60

Query: 61  GQVCSSHRLEGEIDEGCELVSGMELSIGEQPDRTVPAHLFKAVKNNNGTAILLLSDLFAF 120
                  R++ + D+ CELV+GMELSIGEQP  ++PA+LFKAVKNNNGTAILLLSDLFAF
Sbjct: 61  -YYSVKGRVKDDDDDACELVNGMELSIGEQPHHSIPAYLFKAVKNNNGTAILLLSDLFAF 120

Query: 121 QDSSITDFAYRVACNGFNVLLPDVFHGDPWKKKQPVEQTAKQDMQRVAEDIGTATKWLVE 180
           QDSSI DFAYR+ACNGFNVLLPD+ + DPW +K P + +AKQ+MQR+AEDIGT TKWL +
Sbjct: 121 QDSSIRDFAYRLACNGFNVLLPDLVNEDPW-EKPPAQHSAKQEMQRIAEDIGTVTKWLTD 180

Query: 181 EFSAAGISTKLGIMGFGYGGGQVIDVLARDQGACFAIGASFYGTNIDPSLGSQVKVPVLL 240
           EFSAAG+S KLGI+GFG+GGG+VIDVLARD+GACF IGAS YGT +DPSL ++VKVPVLL
Sbjct: 181 EFSAAGLSRKLGIIGFGHGGGRVIDVLARDKGACFLIGASLYGTEVDPSLATKVKVPVLL 240

Query: 241 ISGDNDPLCPVSVMKDLEKGIGKGSKLVIFGGRGHGFFHKPGSPEEDEDAERAFLMLRNW 294
           ISGD+DPLCPVSVMKDLEKG+G+GSKLVIF GRGHGF H PGSPEED+DAE+AFLMLRNW
Sbjct: 241 ISGDDDPLCPVSVMKDLEKGMGEGSKLVIFEGRGHGFVHNPGSPEEDDDAEQAFLMLRNW 300

BLAST of Moc11g31760 vs. ExPASy TrEMBL
Match: A0A1S3CB89 (uncharacterized protein LOC103498731 OS=Cucumis melo OX=3656 GN=LOC103498731 PE=4 SV=1)

HSP 1 Score: 408.7 bits (1049), Expect = 2.1e-110
Identity = 211/309 (68.28%), Postives = 243/309 (78.64%), Query Frame = 0

Query: 1   MGLASAGAAQMISL--------HIGTP-------FPSPLHLRKC-NMGVWKWKVKREKKG 60
           MG ASAG AQ+ISL         +  P       F  PL   K  N G WKWK K     
Sbjct: 1   MGFASAG-AQLISLPPTGSSSRRVEKPIQPPYSQFAYPLPSWKYNNWGDWKWKGKLR--- 60

Query: 61  GQVCSSHRLEGEIDEGCELVSGMELSIGEQPDRTVPAHLFKAVKNNNGTAILLLSDLFAF 120
                  R++ + D+ CELV+GMELSIGEQP  ++PA+LFKAVKNNNGTAILLLSDLFAF
Sbjct: 61  -YYSVKGRVKDDDDDACELVNGMELSIGEQPHHSIPAYLFKAVKNNNGTAILLLSDLFAF 120

Query: 121 QDSSITDFAYRVACNGFNVLLPDVFHGDPWKKKQPVEQTAKQDMQRVAEDIGTATKWLVE 180
           QDSSI DFAYR+ACNGFNVLLPD+ + DPW +K P + +AKQ+MQR+AEDIGT TKWL +
Sbjct: 121 QDSSIRDFAYRLACNGFNVLLPDLVNEDPW-EKPPAQHSAKQEMQRIAEDIGTVTKWLTD 180

Query: 181 EFSAAGISTKLGIMGFGYGGGQVIDVLARDQGACFAIGASFYGTNIDPSLGSQVKVPVLL 240
           EFSAAG+S KLGI+GFG+GGG+VIDVLARD+GACF IGAS YGT +DPSL ++VKVPVLL
Sbjct: 181 EFSAAGLSRKLGIIGFGHGGGRVIDVLARDKGACFLIGASLYGTEVDPSLATKVKVPVLL 240

Query: 241 ISGDNDPLCPVSVMKDLEKGIGKGSKLVIFGGRGHGFFHKPGSPEEDEDAERAFLMLRNW 294
           ISGD+DPLCPVSVMKDLEKG+G+GSKLVIF GRGHGF H PGSPEED+DAE+AFLMLRNW
Sbjct: 241 ISGDDDPLCPVSVMKDLEKGMGEGSKLVIFEGRGHGFVHNPGSPEEDDDAEQAFLMLRNW 300

BLAST of Moc11g31760 vs. ExPASy TrEMBL
Match: A0A6J1I908 (carboxymethylenebutenolidase homolog OS=Cucurbita maxima OX=3661 GN=LOC111472595 PE=4 SV=1)

HSP 1 Score: 398.3 bits (1022), Expect = 2.8e-107
Identity = 210/306 (68.63%), Postives = 242/306 (79.08%), Query Frame = 0

Query: 1   MGLASAGAAQMISL--------HIGTPF-PSPLHLRKCNMGVWKWKVKREKKGGQVC-SS 60
           MG ASAG AQ IS          +G PF PS       +  + + K KREKKGG+VC S+
Sbjct: 1   MGSASAG-AQWISAPPTGRSGRRMGIPFQPSFRCAFPISNNIGQLKGKREKKGGKVCYSA 60

Query: 61  HRLEGEID--EGCELVSGMELSIGEQPDRTVPAHLFKAVKNNNGTAILLLSDLFAFQDSS 120
             ++ EID  E CELVSGMELSIGE+   ++ AHLFKAVKNNNGTAILLL+DLF+FQDSS
Sbjct: 61  EGVKDEIDAEEACELVSGMELSIGEEAHHSITAHLFKAVKNNNGTAILLLTDLFSFQDSS 120

Query: 121 ITDFAYRVACNGFNVLLPDVFHGDPWKKKQPVEQTAKQDMQRVAEDIGTATKWLVEEFSA 180
           + DFAYR+ACNGFNVLLPDVFHGD  ++            Q+  EDIG ATKWLV+EF A
Sbjct: 121 VRDFAYRLACNGFNVLLPDVFHGDSCEE------------QKAVEDIGAATKWLVDEFLA 180

Query: 181 AGISTKLGIMGFGYGGGQVIDVLARDQGACFAIGASFYGTNIDPSLGSQVKVPVLLISGD 240
           AGIS KLGI+GFGYGGG+VIDVLA+DQG+ F+IGAS YGT ++PSL ++V+VPVLLISGD
Sbjct: 181 AGISRKLGIVGFGYGGGRVIDVLAQDQGSYFSIGASLYGTEVNPSLATKVEVPVLLISGD 240

Query: 241 NDPLCPVSVMKDLEKGIGKGSKLVIFGGRGHGFFHKPGSPEEDEDAERAFLMLRNWLHDG 295
           NDPLCPVSVMKDLEKG+G+GSKLVIFGGRGHGF HKPGSPEEDEDAE+AFLMLRNWLHDG
Sbjct: 241 NDPLCPVSVMKDLEKGMGEGSKLVIFGGRGHGFVHKPGSPEEDEDAEQAFLMLRNWLHDG 293

BLAST of Moc11g31760 vs. TAIR 10
Match: AT1G35420.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 286.2 bits (731), Expect = 3.0e-77
Identity = 134/241 (55.60%), Postives = 176/241 (73.03%), Query Frame = 0

Query: 54  EGEIDEGCELVSGMELSIGEQPDRTVPAHLFKAVKNNNGTAILLLSDLFAFQDSSITDFA 113
           + E +E CELV+G E+S+       V  +L  AVKNNNGT +LLLSD+F FQDS+  DFA
Sbjct: 75  DDEEEEACELVNGTEVSVD-----GVEGYLLTAVKNNNGTGLLLLSDVFGFQDSATRDFA 134

Query: 114 YRVACNGFNVLLPDVFHGDPWKKKQPVEQTAK----QDMQRVAEDIGTATKWLVEEFSAA 173
           YRVACNG+NVL+PD+F GDPW K +P  +  +     D  R+ +D  T TKW+VEEF+AA
Sbjct: 135 YRVACNGYNVLVPDLFRGDPWSKNRPKSEYEEWRRGHDPNRIRQDTTTFTKWMVEEFAAA 194

Query: 174 GISTKLGIMGFGYGGGQVIDVLARDQGACFAIGASFYGTNIDPSLGSQVKVPVLLISGDN 233
           GIS KLG+MGF +GGG+V+DVLA D+   F+ G SFYGT ID ++   VKVPVL I+GD 
Sbjct: 195 GISKKLGVMGFCFGGGRVVDVLATDESGYFSTGVSFYGTRIDSAVAGDVKVPVLFIAGDR 254

Query: 234 DPLCPVSVMKDLEKGIGKGSKLVIFGGRGHGFFHKPGSPEEDEDAERAFLMLRNWLHDGL 291
           DPLC V  + ++E+ IG+GSK+V++ GRGHGF H+P +PE+D DAE AF ++RNWLH  L
Sbjct: 255 DPLCEVKGLYEIEEKIGEGSKVVVYEGRGHGFVHRPETPEDDRDAEEAFALMRNWLHHHL 310

BLAST of Moc11g31760 vs. TAIR 10
Match: AT1G35420.2 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 286.2 bits (731), Expect = 3.0e-77
Identity = 134/241 (55.60%), Postives = 176/241 (73.03%), Query Frame = 0

Query: 54  EGEIDEGCELVSGMELSIGEQPDRTVPAHLFKAVKNNNGTAILLLSDLFAFQDSSITDFA 113
           + E +E CELV+G E+S+       V  +L  AVKNNNGT +LLLSD+F FQDS+  DFA
Sbjct: 80  DDEEEEACELVNGTEVSVD-----GVEGYLLTAVKNNNGTGLLLLSDVFGFQDSATRDFA 139

Query: 114 YRVACNGFNVLLPDVFHGDPWKKKQPVEQTAK----QDMQRVAEDIGTATKWLVEEFSAA 173
           YRVACNG+NVL+PD+F GDPW K +P  +  +     D  R+ +D  T TKW+VEEF+AA
Sbjct: 140 YRVACNGYNVLVPDLFRGDPWSKNRPKSEYEEWRRGHDPNRIRQDTTTFTKWMVEEFAAA 199

Query: 174 GISTKLGIMGFGYGGGQVIDVLARDQGACFAIGASFYGTNIDPSLGSQVKVPVLLISGDN 233
           GIS KLG+MGF +GGG+V+DVLA D+   F+ G SFYGT ID ++   VKVPVL I+GD 
Sbjct: 200 GISKKLGVMGFCFGGGRVVDVLATDESGYFSTGVSFYGTRIDSAVAGDVKVPVLFIAGDR 259

Query: 234 DPLCPVSVMKDLEKGIGKGSKLVIFGGRGHGFFHKPGSPEEDEDAERAFLMLRNWLHDGL 291
           DPLC V  + ++E+ IG+GSK+V++ GRGHGF H+P +PE+D DAE AF ++RNWLH  L
Sbjct: 260 DPLCEVKGLYEIEEKIGEGSKVVVYEGRGHGFVHRPETPEDDRDAEEAFALMRNWLHHHL 315

BLAST of Moc11g31760 vs. TAIR 10
Match: AT3G23600.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 59.3 bits (142), Expect = 6.0e-09
Identity = 50/197 (25.38%), Postives = 88/197 (44.67%), Query Frame = 0

Query: 95  ILLLSDLFAFQDSSITDFAYRVACNGFNVLLPDVFHGDPWKKKQPVEQTAKQDMQRVAED 154
           +LL+SD+F F+  ++   A +VA +GF V++PD F GDP+                  + 
Sbjct: 42  VLLISDIFGFEAPNLRALADKVAASGFYVVVPDYFGGDPYNPSNQDRPIPVWIKDHGCDK 101

Query: 155 IGTATKWLVEEFSAAGISTKLGIMGFGYGGGQVIDVLARDQ-GACFAIGASFYGTNIDPS 214
               TK ++E     GI T +G  G  +G   V+++   +   A   +  SF   N+D  
Sbjct: 102 GFENTKPVLETIKNKGI-TAIGAAGMCWGAKVVVELSKEELIQAAVLLHPSF--VNVDDI 161

Query: 215 LGSQVKVPVLLISGDNDPLCPVSVMKDLEKGIGK----GSKLVIFGGRGHGFF--HKPGS 274
            G   K P+ ++  + D + P +++K  E+ +       S + I     HG+   +    
Sbjct: 162 KGG--KAPIAILGAEIDQMSPPALLKQFEEILSSKPEVNSYVKIHPKVSHGWTVRYNIDE 221

Query: 275 PEEDEDAERAFLMLRNW 285
           PE  + AE A   + +W
Sbjct: 222 PEAVKAAEEAHKEMLDW 233

BLAST of Moc11g31760 vs. TAIR 10
Match: AT3G23600.2 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 55.8 bits (133), Expect = 6.6e-08
Identity = 50/197 (25.38%), Postives = 88/197 (44.67%), Query Frame = 0

Query: 95  ILLLSDLFAFQDSSITDFAYRVACNGFNVLLPDVFHGDPWKKKQPVEQTAKQDMQRVAED 154
           +LL+SD+F F+  ++   A +VA +GF V++PD F GDP+                  + 
Sbjct: 42  VLLISDIFGFEAPNLRALADKVAASGFYVVVPDYFGGDPYNPSNQDRPIPVWIKDHGCDK 101

Query: 155 IGTATKWLVEEFSAAGISTKLGIMGFGYGGGQVIDVLARDQ-GACFAIGASFYGTNIDPS 214
               TK ++E     GI T +G  G  +G   V+++   +   A   +  SF   N+D  
Sbjct: 102 GFENTKPVLETIKNKGI-TAIGAAGMCWG---VVELSKEELIQAAVLLHPSF--VNVDDI 161

Query: 215 LGSQVKVPVLLISGDNDPLCPVSVMKDLEKGIGK----GSKLVIFGGRGHGFF--HKPGS 274
            G   K P+ ++  + D + P +++K  E+ +       S + I     HG+   +    
Sbjct: 162 KGG--KAPIAILGAEIDQMSPPALLKQFEEILSSKPEVNSYVKIHPKVSHGWTVRYNIDE 221

Query: 275 PEEDEDAERAFLMLRNW 285
           PE  + AE A   + +W
Sbjct: 222 PEAVKAAEEAHKEMLDW 230

BLAST of Moc11g31760 vs. TAIR 10
Match: AT3G23570.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 53.5 bits (127), Expect = 3.3e-07
Identity = 49/202 (24.26%), Postives = 87/202 (43.07%), Query Frame = 0

Query: 94  AILLLSDLFAFQDSSITDFAYRVACNGFNVLLPDVFHGDPWKKKQPVEQTAKQDMQRVAE 153
           A+LL+  +F ++  ++   A +VA  GF  ++PD FHGDP+  +               E
Sbjct: 41  AVLLVPHVFGYETPNLRKLADKVAEAGFYAVVPDFFHGDPYNPENQDRPFPIWMKDHELE 100

Query: 154 DIGTATKWLVEEFSAAGISTKLGIMGFGYGGGQVIDVLARDQGACFAIGASFYGTNIDPS 213
                +K +VE     GI T +G  GF +G    ++ LA+++     +        +D  
Sbjct: 101 KGFEESKPIVEALKNKGI-TSIGAAGFCWGAKVAVE-LAKEKLVDATVLLHPARVTVDDI 160

Query: 214 LGSQVKVPVLLISGDNDPLCPVSVMKDLEKGIGK----GSKLVIFGGRGHGFF--HKPGS 273
              +V +P+ ++  + D + P  +++  E  +       S + IF    HG+   +    
Sbjct: 161 --KEVNLPIAVLGAEIDQVSPPELVRQFEDILASKPQVKSFVKIFPRCKHGWTVRYNEND 220

Query: 274 PEEDEDAERAFLMLRNWLHDGL 290
           P E E A  A   +  WL D L
Sbjct: 221 PSEVEAAMEAHKDMLAWLIDYL 238