Moc10g02660 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc10g02660
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
DescriptionN(4)-(Beta-n-acetylglucosaminyl)-l-asparaginase, putative
Locationchr10: 1808517 .. 1811192 (+)
RNA-Seq ExpressionMoc10g02660
SyntenyMoc10g02660
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAACTCTTTCCCCAAGATCCCAACAATCAGTTTCGAGCCTGCGTCTTGCTGCTTGTATTGCTCTTGCAGGTAGAACCCACCACCGTATTTTCTCTGTTCAATGTCATTTGCAATTTTCCGAGTTTGGTGTCTGAATCAACGTAAAATTTGGAAGAAAACTTTGAGAGTTTCAAGCACCCAGTTATTTCCTTAGCAAAATTTTGAAGCCCGATTCATCCATTTTCTTTTCTAATGGCGGTGGATTTTGGGTTGGCTGCAGATCAGCCAATCTATACAATTGAATGGGGAATTTTCCCATCTCCAAATTTGTTTTAAGTTCTCTTCTATTCCCTAAATTCCTGGTTGCAGAATTTCGCCTGCTTATTTTCTTCACTGAAAGCTGTATTGATTGTTGAGTTATCAGGTTACTGGACGTGTATCTGGAGAGCCAGGCTCGTACCCTTTGGTTGTAAGCACATGGCCCTTTTTGGAGGCTGTAGAAAGGGCATGGGATGCTGTTAATAATGGCTATTCAGCAGTTGATGCTGTTGTTGAAGGATGTTCCGCCTGTGAAGAACTGAGATGTGATGGTACAGGTGAGAACTTTAATTTGATAAAGAAATTGATGTGGGTCTAAATAAATATTTGAGTTTATTACCTCAGTCATTTCTATTGCTTAGCTATGGTGTTTGCCTCTTTGATTCTCTCTTATGTTTAGTATTTATTGTTCTCAAAAGTTAATTTTTGCTGGCGTTTGCTTCTTCACTTGTCTTAATTTGTAAAGCACTTCACTGGGTGCTTTCTTTATATTGAAAAATATCATGGATTTGCCACAAGCAAAAGCTGGAGTAGAGATTTAATTTGATATAGGAATATTTTCAAGACGAAAGTATGGATACTTCATGAGTATGGATCACTATTGCACCTCGATACTGTATTCGTGAGACTCATATTTTGCAGCAATGATTTAGAAATTTTGCAAACTAATAACACTTCTATATGGGTTTGATGCATTGATATTCATCCATAATTCTTTATGAATAGTTGGACCTGGTGGAAGTCCAGATGAGAATGGAGAAACAACTATTGATGCCATGGTTATGGATGGGGTAAACTTCAGTTTACCTGTTTCTCTCTTGCTTTCTATACCATTTATTTCCTGTTGCTTGTCTTTGGGCATCAAAATAAGGGTTCCACTTTATATTTTACTAAGGATATGCATAGATGACGATGGAAGTTGGAGCTGTCGCCGCTATGAGGTATATTAAGGATGGTATAAGAGCTGCAAGATTAGTGATGAGACATACCAAACATACTTTACTTGTTGGAGAGAAGGCCTCTGCTTTCTCCCTTTCTATGGGTCTTCCTGGACCGACGAATCTTAGTTCGCCAGAGTCCATAGAAAAGTGGAATACGTGGAAAGAGAACAATTGCCAACCTAATTTTTGGAAAAATGTTGTGCCTCTCAATAGTTGCGGCCCTTATTACTATGAGGACCCTCTGGTCGTCACTGGGAAGATGTGTCGAGGAGAAGATCAGAGGGGAACTGTTGAACTAAGATCAGATCATGTTGGTCTTCACAGTCATGATACCATATCAATGGCAGTAATTGATAAGGTATGTTGATAAAATGAAATGAATTTTCTTTTGTCATTGAGTTCATGAATATTACTGGATTTGTTTCTTGGAATAGTTTGGGCACATTGCTGTTGGAACATCCACCAATGGAGCCACCTTCAAGATCCCAGGCAGGTAAGTTTTGATAGCTGCCTACTCTTTACTTTGTTGATAAAGCTTCACTCTTATCTTTACACAGAGAAATATGAGATTCGAGTAGGTCAAAACTTCATTTAAAATGTGAATTTGAAGATTCATATATTTGATACAAACTCCCAAGTCAGATTATGAAATCATTGCCACTTCCATTTGTTATTAGCGCCATTTTCTTGTCAGTTGTCACACAATAAACTAGATGTTGTGCCTCTGTGAATTAGCTTTCTCTGCTACAGGGTGGGTGATGGACCTATAGCAGGATCTTCGGCCTATGCAGACGGTGATATTGGTGCTTGTGGAGCAACCGGGGATGGCGACATCATGATGCGATTCCTCCCATGGTAACACAAACCACAAATTTCTTACAGATGACAAAAACTGAGAACTTTCCATCTGAGACAGTGTTTTGAACCTTGATTTGCTCAGTGCTACACTTTTTAAGCTCAGCCCAAGTCTAAAGGCTGAATCCTAGTGTTATGTTCATAAACAAAACAATTTCTACCCTTCATTTATACAACTCTGCTTTCTATAAAACCCAATGAATAAGCCTTGAGATGTTCTAACTTCTATGATATCAACAAATTGATTTGGGTCTATTTGCTTGTTCATTAGTTCTTCAAAATCATTCTCACTATTCCCATCTTTAACTAAATTTCTGCTTTCGGTTCAATATCCGAAATACTTCCAACCACTTGTTACAGCTATCAAGTTGTCGAGAGTATGCGACTGGGGATGGAGCCGAAGGATGCTGCTAAAGATGCAATATCACGGATCACAAGAAAGTTCCCAGACTTTGTTGGAGCTATTTTTGCTGTTAACAAAACTGGCACTCATGCCGGGGCTTGCCATGGATGGACATTTCAATACTCGGTCAGAAACGCAGAAATGCTTCGTGCCGAGGTTTTCACTGTACTTCCTTGA

mRNA sequence

ATGGAACTCTTTCCCCAAGATCCCAACAATCAGTTTCGAGCCTGCGTCTTGCTGCTTGTATTGCTCTTGCAGGTTACTGGACGTGTATCTGGAGAGCCAGGCTCGTACCCTTTGGTTGTAAGCACATGGCCCTTTTTGGAGGCTGTAGAAAGGGCATGGGATGCTGTTAATAATGGCTATTCAGCAGTTGATGCTGTTGTTGAAGGATGTTCCGCCTGTGAAGAACTGAGATGTGATGGTACAGTTGGACCTGGTGGAAGTCCAGATGAGAATGGAGAAACAACTATTGATGCCATGGTTATGGATGGGATGACGATGGAAGTTGGAGCTGTCGCCGCTATGAGGTATATTAAGGATGGTATAAGAGCTGCAAGATTAGTGATGAGACATACCAAACATACTTTACTTGTTGGAGAGAAGGCCTCTGCTTTCTCCCTTTCTATGGGTCTTCCTGGACCGACGAATCTTAGTTCGCCAGAGTCCATAGAAAAGTGGAATACGTGGAAAGAGAACAATTGCCAACCTAATTTTTGGAAAAATGTTGTGCCTCTCAATAGTTGCGGCCCTTATTACTATGAGGACCCTCTGGTCGTCACTGGGAAGATGTGTCGAGGAGAAGATCAGAGGGGAACTGTTGAACTAAGATCAGATCATGTTGGTCTTCACAGTCATGATACCATATCAATGGCAGTAATTGATAAGTTTGGGCACATTGCTGTTGGAACATCCACCAATGGAGCCACCTTCAAGATCCCAGGCAGGGTGGGTGATGGACCTATAGCAGGATCTTCGGCCTATGCAGACGGTGATATTGGTGCTTGTGGAGCAACCGGGGATGGCGACATCATGATGCGATTCCTCCCATGCTATCAAGTTGTCGAGAGTATGCGACTGGGGATGGAGCCGAAGGATGCTGCTAAAGATGCAATATCACGGATCACAAGAAAGTTCCCAGACTTTGTTGGAGCTATTTTTGCTGTTAACAAAACTGGCACTCATGCCGGGGCTTGCCATGGATGGACATTTCAATACTCGGTCAGAAACGCAGAAATGCTTCGTGCCGAGGTTTTCACTGTACTTCCTTGA

Coding sequence (CDS)

ATGGAACTCTTTCCCCAAGATCCCAACAATCAGTTTCGAGCCTGCGTCTTGCTGCTTGTATTGCTCTTGCAGGTTACTGGACGTGTATCTGGAGAGCCAGGCTCGTACCCTTTGGTTGTAAGCACATGGCCCTTTTTGGAGGCTGTAGAAAGGGCATGGGATGCTGTTAATAATGGCTATTCAGCAGTTGATGCTGTTGTTGAAGGATGTTCCGCCTGTGAAGAACTGAGATGTGATGGTACAGTTGGACCTGGTGGAAGTCCAGATGAGAATGGAGAAACAACTATTGATGCCATGGTTATGGATGGGATGACGATGGAAGTTGGAGCTGTCGCCGCTATGAGGTATATTAAGGATGGTATAAGAGCTGCAAGATTAGTGATGAGACATACCAAACATACTTTACTTGTTGGAGAGAAGGCCTCTGCTTTCTCCCTTTCTATGGGTCTTCCTGGACCGACGAATCTTAGTTCGCCAGAGTCCATAGAAAAGTGGAATACGTGGAAAGAGAACAATTGCCAACCTAATTTTTGGAAAAATGTTGTGCCTCTCAATAGTTGCGGCCCTTATTACTATGAGGACCCTCTGGTCGTCACTGGGAAGATGTGTCGAGGAGAAGATCAGAGGGGAACTGTTGAACTAAGATCAGATCATGTTGGTCTTCACAGTCATGATACCATATCAATGGCAGTAATTGATAAGTTTGGGCACATTGCTGTTGGAACATCCACCAATGGAGCCACCTTCAAGATCCCAGGCAGGGTGGGTGATGGACCTATAGCAGGATCTTCGGCCTATGCAGACGGTGATATTGGTGCTTGTGGAGCAACCGGGGATGGCGACATCATGATGCGATTCCTCCCATGCTATCAAGTTGTCGAGAGTATGCGACTGGGGATGGAGCCGAAGGATGCTGCTAAAGATGCAATATCACGGATCACAAGAAAGTTCCCAGACTTTGTTGGAGCTATTTTTGCTGTTAACAAAACTGGCACTCATGCCGGGGCTTGCCATGGATGGACATTTCAATACTCGGTCAGAAACGCAGAAATGCTTCGTGCCGAGGTTTTCACTGTACTTCCTTGA

Protein sequence

MELFPQDPNNQFRACVLLLVLLLQVTGRVSGEPGSYPLVVSTWPFLEAVERAWDAVNNGYSAVDAVVEGCSACEELRCDGTVGPGGSPDENGETTIDAMVMDGMTMEVGAVAAMRYIKDGIRAARLVMRHTKHTLLVGEKASAFSLSMGLPGPTNLSSPESIEKWNTWKENNCQPNFWKNVVPLNSCGPYYYEDPLVVTGKMCRGEDQRGTVELRSDHVGLHSHDTISMAVIDKFGHIAVGTSTNGATFKIPGRVGDGPIAGSSAYADGDIGACGATGDGDIMMRFLPCYQVVESMRLGMEPKDAAKDAISRITRKFPDFVGAIFAVNKTGTHAGACHGWTFQYSVRNAEMLRAEVFTVLP
Homology
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BLAST of Moc10g02660 vs. NCBI nr
Match: XP_022141259.1 (probable isoaspartyl peptidase/L-asparaginase 3 isoform X1 [Momordica charantia])

HSP 1 Score: 745.7 bits (1924), Expect = 1.8e-211
Identity = 361/361 (100.00%), Postives = 361/361 (100.00%), Query Frame = 0 

Query: 1   MELFPQDPNNQFRACVLLLVLLLQVTGRVSGEPGSYPLVVSTWPFLEAVERAWDAVNNGY 60
           MELFPQDPNNQFRACVLLLVLLLQVTGRVSGEPGSYPLVVSTWPFLEAVERAWDAVNNGY
Sbjct: 1   MELFPQDPNNQFRACVLLLVLLLQVTGRVSGEPGSYPLVVSTWPFLEAVERAWDAVNNGY 60

Query: 61  SAVDAVVEGCSACEELRCDGTVGPGGSPDENGETTIDAMVMDGMTMEVGAVAAMRYIKDG 120
           SAVDAVVEGCSACEELRCDGTVGPGGSPDENGETTIDAMVMDGMTMEVGAVAAMRYIKDG
Sbjct: 61  SAVDAVVEGCSACEELRCDGTVGPGGSPDENGETTIDAMVMDGMTMEVGAVAAMRYIKDG 120

Query: 121 IRAARLVMRHTKHTLLVGEKASAFSLSMGLPGPTNLSSPESIEKWNTWKENNCQPNFWKN 180
           IRAARLVMRHTKHTLLVGEKASAFSLSMGLPGPTNLSSPESIEKWNTWKENNCQPNFWKN
Sbjct: 121 IRAARLVMRHTKHTLLVGEKASAFSLSMGLPGPTNLSSPESIEKWNTWKENNCQPNFWKN 180

Query: 181 VVPLNSCGPYYYEDPLVVTGKMCRGEDQRGTVELRSDHVGLHSHDTISMAVIDKFGHIAV 240
           VVPLNSCGPYYYEDPLVVTGKMCRGEDQRGTVELRSDHVGLHSHDTISMAVIDKFGHIAV
Sbjct: 181 VVPLNSCGPYYYEDPLVVTGKMCRGEDQRGTVELRSDHVGLHSHDTISMAVIDKFGHIAV 240

Query: 241 GTSTNGATFKIPGRVGDGPIAGSSAYADGDIGACGATGDGDIMMRFLPCYQVVESMRLGM 300
           GTSTNGATFKIPGRVGDGPIAGSSAYADGDIGACGATGDGDIMMRFLPCYQVVESMRLGM
Sbjct: 241 GTSTNGATFKIPGRVGDGPIAGSSAYADGDIGACGATGDGDIMMRFLPCYQVVESMRLGM 300

Query: 301 EPKDAAKDAISRITRKFPDFVGAIFAVNKTGTHAGACHGWTFQYSVRNAEMLRAEVFTVL 360
           EPKDAAKDAISRITRKFPDFVGAIFAVNKTGTHAGACHGWTFQYSVRNAEMLRAEVFTVL
Sbjct: 301 EPKDAAKDAISRITRKFPDFVGAIFAVNKTGTHAGACHGWTFQYSVRNAEMLRAEVFTVL 360

Query: 361 P 362
           P
Sbjct: 361 P 361

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BLAST of Moc10g02660 vs. NCBI nr
Match: XP_022985489.1 (probable isoaspartyl peptidase/L-asparaginase 3 [Cucurbita maxima])

HSP 1 Score: 646.0 bits (1665), Expect = 2.0e-181
Identity = 313/361 (86.70%), Postives = 330/361 (91.41%), Query Frame = 0 

Query: 1   MELFPQDPNNQFRACVLLLVLLLQVTGRVSGEPGSYPLVVSTWPFLEAVERAWDAVNNGY 60
           MEL+  D NN+FRA  LLLVLL QVTGRV+GE GSYPLVVSTWPFLEAVERAW AVNNG 
Sbjct: 1   MELYAHDSNNRFRASALLLVLLFQVTGRVAGESGSYPLVVSTWPFLEAVERAWSAVNNGS 60

Query: 61  SAVDAVVEGCSACEELRCDGTVGPGGSPDENGETTIDAMVMDGMTMEVGAVAAMRYIKDG 120
           SAVDAVV+GCSACEELRCDGTVGPGGSPDENGETTIDAMVMDG+TMEVGAVAAMRYIKDG
Sbjct: 61  SAVDAVVDGCSACEELRCDGTVGPGGSPDENGETTIDAMVMDGVTMEVGAVAAMRYIKDG 120

Query: 121 IRAARLVMRHTKHTLLVGEKASAFSLSMGLPGPTNLSSPESIEKWNTWKENNCQPNFWKN 180
           I+AARLVMRHT+HTLLVGEKASAFS+SMGLPGP +LSS ESIEKWN WKENNCQPNFWKN
Sbjct: 121 IKAARLVMRHTQHTLLVGEKASAFSISMGLPGPEDLSSSESIEKWNKWKENNCQPNFWKN 180

Query: 181 VVPLNSCGPYYYEDPLVVTGKMCRGEDQRGTVELRSDHVGLHSHDTISMAVIDKFGHIAV 240
           VVP+NSCGPYY  D +VV    C G+D   TV LRS+H GLHSHDTISMAVIDKFGHIAV
Sbjct: 181 VVPVNSCGPYYSNDLMVVAETTCSGDDPMRTVGLRSNHFGLHSHDTISMAVIDKFGHIAV 240

Query: 241 GTSTNGATFKIPGRVGDGPIAGSSAYADGDIGACGATGDGDIMMRFLPCYQVVESMRLGM 300
           GTSTNGATFKIPGRVGDGPIAGSSAYAD DIGACGATGDGDIMMRFLPCYQVVESMRLGM
Sbjct: 241 GTSTNGATFKIPGRVGDGPIAGSSAYADSDIGACGATGDGDIMMRFLPCYQVVESMRLGM 300

Query: 301 EPKDAAKDAISRITRKFPDFVGAIFAVNKTGTHAGACHGWTFQYSVRNAEMLRAEVFTVL 360
           +PKDAAKDAISRI RKFPDF+GAIFAVNK GTHAGACHGWTF+YSVR+ EM  AEVFTVL
Sbjct: 301 DPKDAAKDAISRIARKFPDFIGAIFAVNKNGTHAGACHGWTFKYSVRSPEMHHAEVFTVL 360

Query: 361 P 362
           P
Sbjct: 361 P 361

[Close]
BLAST of Moc10g02660 vs. NCBI nr
Match: XP_038900285.1 (probable isoaspartyl peptidase/L-asparaginase 3 isoform X1 [Benincasa hispida])

HSP 1 Score: 641.3 bits (1653), Expect = 4.8e-180
Identity = 310/361 (85.87%), Postives = 329/361 (91.14%), Query Frame = 0 

Query: 1   MELFPQDPNNQFRACVLLLVLLLQVTGRVSGEPGSYPLVVSTWPFLEAVERAWDAVNNGY 60
           MELF  D +N+ RA  LLL+L  Q+TGRV+GE G YPLVVSTWPFLEAVERAW AVNNGY
Sbjct: 1   MELFALDSDNRLRASALLLLLFFQMTGRVAGESGLYPLVVSTWPFLEAVERAWSAVNNGY 60

Query: 61  SAVDAVVEGCSACEELRCDGTVGPGGSPDENGETTIDAMVMDGMTMEVGAVAAMRYIKDG 120
           SAVD+VVEGCSACEELRCDGTVGPGGSPDENGETTIDAMVMDG+TMEVGAVAAMRYIKDG
Sbjct: 61  SAVDSVVEGCSACEELRCDGTVGPGGSPDENGETTIDAMVMDGVTMEVGAVAAMRYIKDG 120

Query: 121 IRAARLVMRHTKHTLLVGEKASAFSLSMGLPGPTNLSSPESIEKWNTWKENNCQPNFWKN 180
           I+AARLVMRHTKHTLLVGEKASAFS+SMGLPGPT+LSSPESIEKWN WKENNCQPNFWKN
Sbjct: 121 IKAARLVMRHTKHTLLVGEKASAFSISMGLPGPTDLSSPESIEKWNKWKENNCQPNFWKN 180

Query: 181 VVPLNSCGPYYYEDPLVVTGKMCRGEDQRGTVELRSDHVGLHSHDTISMAVIDKFGHIAV 240
           VVP+NSCGPY+    L+V    C G+D R  VELRS+H GLHSHDTISMAVIDKFG+IAV
Sbjct: 181 VVPVNSCGPYHSNGLLLVAETTCPGDDPRRAVELRSNHFGLHSHDTISMAVIDKFGYIAV 240

Query: 241 GTSTNGATFKIPGRVGDGPIAGSSAYADGDIGACGATGDGDIMMRFLPCYQVVESMRLGM 300
           GTSTNGATFKIPGRVGDGPIAGSSAYAD DIGACGATGDGDIMMRFLPCYQVVESMRLGM
Sbjct: 241 GTSTNGATFKIPGRVGDGPIAGSSAYADSDIGACGATGDGDIMMRFLPCYQVVESMRLGM 300

Query: 301 EPKDAAKDAISRITRKFPDFVGAIFAVNKTGTHAGACHGWTFQYSVRNAEMLRAEVFTVL 360
           +PKDAAKDAISRI RKFPDFVGAIFAVNK GTHAGACHGWTFQYSVR+ EM R E+FTV 
Sbjct: 301 KPKDAAKDAISRIARKFPDFVGAIFAVNKNGTHAGACHGWTFQYSVRSPEMYRTEIFTVH 360

Query: 361 P 362
           P
Sbjct: 361 P 361

[Close]
BLAST of Moc10g02660 vs. NCBI nr
Match: KAG6576819.1 (putative isoaspartyl peptidase/L-asparaginase 3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 636.7 bits (1641), Expect = 1.2e-178
Identity = 309/353 (87.54%), Postives = 325/353 (92.07%), Query Frame = 0 

Query: 9   NNQFRACVLLLVLLLQVTGRVSGEPGSYPLVVSTWPFLEAVERAWDAVNNGYSAVDAVVE 68
           NN+FRA  LLLVLL QVTGRV+GE GSYPLVVSTWPFLEAVERAW AVNNG SAVDAVVE
Sbjct: 7   NNRFRASALLLVLLFQVTGRVAGESGSYPLVVSTWPFLEAVERAWSAVNNGSSAVDAVVE 66

Query: 69  GCSACEELRCDGTVGPGGSPDENGETTIDAMVMDGMTMEVGAVAAMRYIKDGIRAARLVM 128
           GCSACEELRCDGTVGPGGSPDENGETTIDAMVMDG+TMEVGAVAAMRYIKDGI+AARLVM
Sbjct: 67  GCSACEELRCDGTVGPGGSPDENGETTIDAMVMDGVTMEVGAVAAMRYIKDGIKAARLVM 126

Query: 129 RHTKHTLLVGEKASAFSLSMGLPGPTNLSSPESIEKWNTWKENNCQPNFWKNVVPLNSCG 188
           RHT+HTLLVGEKASAFS+SMGLPGP +LSS ESIEKWN WKENNCQPNFWKNVVP+NSCG
Sbjct: 127 RHTQHTLLVGEKASAFSISMGLPGPEDLSSSESIEKWNKWKENNCQPNFWKNVVPVNSCG 186

Query: 189 PYYYEDPLVVTGKMCRGEDQRGTVELRSDHVGLHSHDTISMAVIDKFGHIAVGTSTNGAT 248
           PY+  D ++V    C G+D   TV LRS+H GLHSHDTISMAVIDKFGHIAVGTSTNGAT
Sbjct: 187 PYHSNDLMLVAETTCSGDDPMRTVGLRSNHFGLHSHDTISMAVIDKFGHIAVGTSTNGAT 246

Query: 249 FKIPGRVGDGPIAGSSAYADGDIGACGATGDGDIMMRFLPCYQVVESMRLGMEPKDAAKD 308
           FKIPGRVGDGPIAGSSAYAD DIGACGATGDGDIMMRFLPCYQVV+SMRLGMEPKDAAKD
Sbjct: 247 FKIPGRVGDGPIAGSSAYADSDIGACGATGDGDIMMRFLPCYQVVKSMRLGMEPKDAAKD 306

Query: 309 AISRITRKFPDFVGAIFAVNKTGTHAGACHGWTFQYSVRNAEMLRAEVFTVLP 362
           AISRI RKFPDF+GAIFAVNK GTHAGACHGWTFQYSVR+ EM  AEVFTVLP
Sbjct: 307 AISRIARKFPDFIGAIFAVNKNGTHAGACHGWTFQYSVRSPEMHHAEVFTVLP 359

[Close]
BLAST of Moc10g02660 vs. NCBI nr
Match: XP_022922742.1 (probable isoaspartyl peptidase/L-asparaginase 3 [Cucurbita moschata])

HSP 1 Score: 636.7 bits (1641), Expect = 1.2e-178
Identity = 309/353 (87.54%), Postives = 324/353 (91.78%), Query Frame = 0 

Query: 9   NNQFRACVLLLVLLLQVTGRVSGEPGSYPLVVSTWPFLEAVERAWDAVNNGYSAVDAVVE 68
           NN+FRA  LLLVLL QVTGRV+GE GSYPLVVSTWPF EAVERAW AVNNG SAVDAVVE
Sbjct: 7   NNRFRASALLLVLLFQVTGRVAGESGSYPLVVSTWPFFEAVERAWSAVNNGSSAVDAVVE 66

Query: 69  GCSACEELRCDGTVGPGGSPDENGETTIDAMVMDGMTMEVGAVAAMRYIKDGIRAARLVM 128
           GCSACEELRCDGTVGPGGSPDENGETTIDAMVMDG+TMEVGAVAAMRYIKDGI+AARLVM
Sbjct: 67  GCSACEELRCDGTVGPGGSPDENGETTIDAMVMDGVTMEVGAVAAMRYIKDGIKAARLVM 126

Query: 129 RHTKHTLLVGEKASAFSLSMGLPGPTNLSSPESIEKWNTWKENNCQPNFWKNVVPLNSCG 188
           RHT+HTLLVGEKASAFS+SMGLPGP +LSS ESIEKWN WKENNCQPNFWKNVVP+NSCG
Sbjct: 127 RHTQHTLLVGEKASAFSISMGLPGPEDLSSSESIEKWNKWKENNCQPNFWKNVVPVNSCG 186

Query: 189 PYYYEDPLVVTGKMCRGEDQRGTVELRSDHVGLHSHDTISMAVIDKFGHIAVGTSTNGAT 248
           PY+  D ++V    C G+D   TV LRS+H GLHSHDTISMAVIDKFGHIAVGTSTNGAT
Sbjct: 187 PYHSNDLMLVAETTCSGDDPMRTVGLRSNHFGLHSHDTISMAVIDKFGHIAVGTSTNGAT 246

Query: 249 FKIPGRVGDGPIAGSSAYADGDIGACGATGDGDIMMRFLPCYQVVESMRLGMEPKDAAKD 308
           FKIPGRVGDGPIAGSSAYAD DIGACGATGDGDIMMRFLPCYQVVESMRLGMEPKDAAKD
Sbjct: 247 FKIPGRVGDGPIAGSSAYADSDIGACGATGDGDIMMRFLPCYQVVESMRLGMEPKDAAKD 306

Query: 309 AISRITRKFPDFVGAIFAVNKTGTHAGACHGWTFQYSVRNAEMLRAEVFTVLP 362
           AISRI RKFPDF+GAIFAVNK GTHAGACHGWTFQYSVR+ EM  AEVFTVLP
Sbjct: 307 AISRIARKFPDFIGAIFAVNKNGTHAGACHGWTFQYSVRSPEMHHAEVFTVLP 359

[Close]
BLAST of Moc10g02660 vs. ExPASy Swiss-Prot
Match: Q56W64 (Probable isoaspartyl peptidase/L-asparaginase 3 OS=Arabidopsis thaliana OX=3702 GN=At5g61540 PE=2 SV=1)

HSP 1 Score: 508.4 bits (1308), Expect = 6.4e-143
Identity = 248/351 (70.66%), Postives = 288/351 (82.05%), Query Frame = 0 

Query: 12  FRACVLLLVLLLQVTGRVSGEPGSYPLVVSTWPFLEAVERAWDAVNNGYSAVDAVVEGCS 71
           F + +LL + LL V      +   +P+VVSTWPFLEAV  AW AV+NG SAV+AVVEGCS
Sbjct: 9   FVSTLLLFLSLLTVADAELVKSDKFPVVVSTWPFLEAVRAAWRAVDNGSSAVEAVVEGCS 68

Query: 72  ACEELRCDGTVGPGGSPDENGETTIDAMVMDGMTMEVGAVAAMRYIKDGIRAARLVMRHT 131
           ACEELRCDGTVGPGGSPDENGET IDA+VMDG+TMEVGAVAAMRY+KDGIRAA LVM+++
Sbjct: 69  ACEELRCDGTVGPGGSPDENGETMIDALVMDGVTMEVGAVAAMRYVKDGIRAAHLVMKYS 128

Query: 132 KHTLLVGEKASAFSLSMGLPGPTNLSSPESIEKWNTWKENNCQPNFWKNVVPLNSCGPYY 191
           +HTLL GE ASAF++SMGLPGP NLSSPES++KW+ WKEN CQPNF KNVVP N CGPY 
Sbjct: 129 QHTLLAGEGASAFAISMGLPGPMNLSSPESVKKWSDWKENQCQPNFRKNVVPANDCGPYK 188

Query: 192 YEDPLV-VTGKMCRGEDQRGTVELRSDHVGLHSHDTISMAVIDKFGHIAVGTSTNGATFK 251
             +  + V         + G +E +   VG H+HDTISMAVID+ GHIAVGTSTNGAT+K
Sbjct: 189 PNNSAMNVFVDKSTESCEMGAIEYKPPLVGPHNHDTISMAVIDRMGHIAVGTSTNGATYK 248

Query: 252 IPGRVGDGPIAGSSAYADGDIGACGATGDGDIMMRFLPCYQVVESMRLGMEPKDAAKDAI 311
           IPGRVGDGPI GSSAYAD ++G CGATGDGD MMRFLPCYQVVESMR GM+P++AAKDAI
Sbjct: 249 IPGRVGDGPIVGSSAYADDEVGGCGATGDGDTMMRFLPCYQVVESMRQGMKPEEAAKDAI 308

Query: 312 SRITRKFPDFVGAIFAVNKTGTHAGACHGWTFQYSVRNAEMLRAEVFTVLP 362
           SRI RKFPDFVGA+ AV+K G+HAGAC+GWTFQYSV+N +M   +VFTVLP
Sbjct: 309 SRIARKFPDFVGAVVAVDKNGSHAGACYGWTFQYSVQNPDMNDVQVFTVLP 359

[Close]
BLAST of Moc10g02660 vs. ExPASy Swiss-Prot
Match: P20933 (N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase OS=Homo sapiens OX=9606 GN=AGA PE=1 SV=2)

HSP 1 Score: 302.8 bits (774), Expect = 5.4e-81
Identity = 173/344 (50.29%), Postives = 214/344 (62.21%), Query Frame = 0 

Query: 16  VLLLVLLLQVTGRVSGEPGSYPLVVSTWPFLEAVERAWDAVNNGYSAVDAVVEGCSACEE 75
           +L+  LL Q   R S      PLVV+TWPF  A E AW A+ +G SA+DAV  GC+ CE 
Sbjct: 10  LLVPFLLCQALVRCS---SPLPLVVNTWPFKNATEAAWRALASGGSALDAVESGCAMCER 69

Query: 76  LRCDGTVGPGGSPDENGETTIDAMVMDGMTMEVGAVAAMRYIKDGIRAARLVMRHTKHTL 135
            +CDG+VG GGSPDE GETT+DAM+MDG TM+VGAV  +R IK+ I  AR V+ HT HTL
Sbjct: 70  EQCDGSVGFGGSPDELGETTLDAMIMDGTTMDVGAVGDLRRIKNAIGVARKVLEHTTHTL 129

Query: 136 LVGEKASAFSLSMGLPGPTNLSSPESIEKWNTWKENNCQPNFWKNVVPLNS--CGPYYYE 195
           LVGE A+ F+ SMG     +LS+  S    + W   NCQPN+W+NV+P  S  CGP  Y+
Sbjct: 130 LVGESATTFAQSMGFIN-EDLSTTASQALHSDWLARNCQPNYWRNVIPDPSKYCGP--YK 189

Query: 196 DPLVVTGKM---CRGEDQRGTVELRSDHVGLHSHDTISMAVIDKFGHIAVGTSTNGATFK 255
            P ++   +      ED RG             HDTI M VI K GHIA GTSTNG  FK
Sbjct: 190 PPGILKQDIPIHKETEDDRG-------------HDTIGMVVIHKTGHIAAGTSTNGIKFK 249

Query: 256 IPGRVGDGPIAGSSAYADGDIGACGATGDGDIMMRFLPCYQVVESMRLGMEPKDAAKDAI 315
           I GRVGD PI G+ AYAD   GA  ATG+GDI+MRFLP YQ VE MR G +P  A +  I
Sbjct: 250 IHGRVGDSPIPGAGAYADDTAGAAAATGNGDILMRFLPSYQAVEYMRRGEDPTIACQKVI 309

Query: 316 SRITRKFPDFVGAIFAVNKTGTHAGACHGWT----FQYSVRNAE 351
           SRI + FP+F GA+   N TG++  AC+  +    F + V N+E
Sbjct: 310 SRIQKHFPEFFGAVICANVTGSYGAACNKLSTFTQFSFMVYNSE 334

[Close]
BLAST of Moc10g02660 vs. ExPASy Swiss-Prot
Match: Q64191 (N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase OS=Mus musculus OX=10090 GN=Aga PE=1 SV=1)

HSP 1 Score: 302.4 bits (773), Expect = 7.0e-81
Identity = 162/324 (50.00%), Postives = 205/324 (63.27%), Query Frame = 0 

Query: 17  LLLVLLLQVTGRVSGEPGSYPLVVSTWPFLEAVERAWDAVNNGYSAVDAVVEGCSACEEL 76
           LLL+LL+     V G     PLVV+TWPF  A E AW  + +G SA+DAV  GC+ CE+ 
Sbjct: 9   LLLLLLVLGMPLVRGS-SPLPLVVNTWPFKNATEAAWWTLLSGGSALDAVENGCAVCEKE 68

Query: 77  RCDGTVGPGGSPDENGETTIDAMVMDGMTMEVGAVAAMRYIKDGIRAARLVMRHTKHTLL 136
           +CDGTVG GGSPDE GETT+DAM+MDG  M+VGAV  +R IK+ I  AR V+ HT HTLL
Sbjct: 69  QCDGTVGFGGSPDEGGETTLDAMIMDGTAMDVGAVGGLRRIKNAIGVARRVLEHTTHTLL 128

Query: 137 VGEKASAFSLSMGLPGPTNLSSPESIEKWNTWKENNCQPNFWKNVVPLNS--CGPYYYED 196
           VG+ A+ F+ SMG     +LS+  S +  + W   NCQPN+W+NV+P  S  CGPY    
Sbjct: 129 VGDSATKFAESMGFTN-EDLSTKTSRDLHSDWLSRNCQPNYWRNVIPDPSKYCGPYKPSG 188

Query: 197 PLVVTGKMCRGEDQRGTVELRSDHVGLHSHDTISMAVIDKFGHIAVGTSTNGATFKIPGR 256
            L            + ++    + V +HSHDTI M VI K GH A GTSTNG  FKIPGR
Sbjct: 189 FL------------KQSISPHKEEVDIHSHDTIGMVVIHKTGHTAAGTSTNGIKFKIPGR 248

Query: 257 VGDGPIAGSSAYADGDIGACGATGDGDIMMRFLPCYQVVESMRLGMEPKDAAKDAISRIT 316
           VGD PI G+ AYAD   GA  ATGDGD ++RFLP YQ VE MR G +P  A +  I RI 
Sbjct: 249 VGDSPIPGAGAYADDTAGAAAATGDGDTLLRFLPSYQAVEYMRGGDDPAIACQKVILRIQ 308

Query: 317 RKFPDFVGAIFAVNKTGTHAGACH 339
           + +P+F GA+   +  G++  AC+
Sbjct: 309 KYYPNFFGAVICASVNGSYGAACN 318

[Close]
BLAST of Moc10g02660 vs. ExPASy Swiss-Prot
Match: B4NWI1 (Putative N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase GE19290 OS=Drosophila yakuba OX=7245 GN=GE19290 PE=3 SV=1)

HSP 1 Score: 301.2 bits (770), Expect = 1.6e-80
Identity = 163/309 (52.75%), Postives = 204/309 (66.02%), Query Frame = 0 

Query: 37  PLVVSTWPFLEAVERAWDAVNNGYSAV----DAVVEGCSACEELRCDGTVGPGGSPDENG 96
           P+V++TW F  A   AW  +      +    +AVVEGCS CE+L+CD TVG GGSPDE G
Sbjct: 60  PMVINTWNFTAANVLAWRILKQSKGGLRQTRNAVVEGCSKCEKLQCDRTVGYGGSPDELG 119

Query: 97  ETTIDAMVMDGMTMEVGAVAAMRYIKDGIRAARLVMRHTKHTLLVGEKASAFSLSMGLPG 156
           ETT+DAMVMDG TMEVGAVA +R IKD I+ AR V+ HT+HT+LVG+ ASAF+ +MG   
Sbjct: 120 ETTLDAMVMDGATMEVGAVAGLRRIKDAIKVARHVLEHTQHTMLVGDAASAFANAMGFES 179

Query: 157 PTNLSSPESIEKWNTWKENNCQPNFWKNV--VPLNSCGPYYYEDPLVVTGKMCRGEDQRG 216
             +L +PES + W  W   NCQPNFWKNV   P  SCGP Y   P  +T    R ++ R 
Sbjct: 180 -ESLVTPESKDMWLQWTAENCQPNFWKNVHPDPKVSCGP-YKPRPTPLT----RWKEDRA 239

Query: 217 TVELRSDHVGLHSHDTISMAVIDKFGHIAVGTSTNGATFKIPGRVGDGPIAGSSAYADGD 276
             E     +G  +HDTI M  ID   +I  GTSTNGA  KIPGRVGD PI G+ AYAD +
Sbjct: 240 RNEY---EIGRKNHDTIGMIAIDVESNIHAGTSTNGARHKIPGRVGDSPIPGAGAYADNE 299

Query: 277 IGACGATGDGDIMMRFLPCYQVVESMRLGMEPKDAAKDAISRITRKFPDFVGAIFAVNKT 336
           +GA  ATGDGD+MMRFLP    VE+MR G  P DAA++++ RI R   DF+GA+ AV++ 
Sbjct: 300 VGAAVATGDGDVMMRFLPSLLAVEAMRAGKPPADAAEESLRRIIRHHKDFMGALIAVDRL 359

Query: 337 GTHAGACHG 340
           G +  AC+G
Sbjct: 360 GRYGAACYG 359

[Close]
BLAST of Moc10g02660 vs. ExPASy Swiss-Prot
Match: P30919 (N(4)-(Beta-N-acetylglucosaminyl)-L-asparaginase OS=Rattus norvegicus OX=10116 GN=Aga PE=1 SV=2)

HSP 1 Score: 300.8 bits (769), Expect = 2.0e-80
Identity = 164/324 (50.62%), Postives = 204/324 (62.96%), Query Frame = 0 

Query: 17  LLLVLLLQVTGRVSGEPGSYPLVVSTWPFLEAVERAWDAVNNGYSAVDAVVEGCSACEEL 76
           LLL L+L +       P   PLVV+TWPF  A E AW  + +G SA+DAV +GC+ CE+ 
Sbjct: 10  LLLPLVLGIPLVRGSNP--LPLVVNTWPFKNATEAAWWTLVSGGSALDAVEKGCAMCEKE 69

Query: 77  RCDGTVGPGGSPDENGETTIDAMVMDGMTMEVGAVAAMRYIKDGIRAARLVMRHTKHTLL 136
           +C GTVG GGSPDE GETT+DAM+MDG  M+VGAV  +R IK+ I  AR V+ HT HTLL
Sbjct: 70  QCGGTVGFGGSPDEVGETTLDAMIMDGTAMDVGAVGGLRRIKNAIGVARKVLEHTTHTLL 129

Query: 137 VGEKASAFSLSMGLPGPTNLSSPESIEKWNTWKENNCQPNFWKNVVPLNS--CGPYYYED 196
           VG+ A+ F++SMG     +LS+  S    + W   NCQPN+W+NV+P  S  CGPY   D
Sbjct: 130 VGDSATKFAVSMGFTS-EDLSTNTSRALHSDWLSRNCQPNYWRNVIPDPSKYCGPYKPPD 189

Query: 197 PLVVTGKMCRGEDQRGTVELRSDHVGLHSHDTISMAVIDKFGHIAVGTSTNGATFKIPGR 256
            L    +  +              V +HSHDTI M VI K GH A GTSTNG  FKIPGR
Sbjct: 190 FLEQNNRAHK-------------EVDIHSHDTIGMVVIHKTGHTAAGTSTNGLKFKIPGR 249

Query: 257 VGDGPIAGSSAYADGDIGACGATGDGDIMMRFLPCYQVVESMRLGMEPKDAAKDAISRIT 316
           VGD PI G+ AYAD   GA  ATGDGD ++RFLP YQ VE MR G +P  A +  ISRI 
Sbjct: 250 VGDSPIPGAGAYADDMAGAAAATGDGDTLLRFLPSYQAVEYMRGGDDPARACQKVISRIQ 309

Query: 317 RKFPDFVGAIFAVNKTGTHAGACH 339
           + +P F GA+   N TG++  AC+
Sbjct: 310 KYYPKFFGAVICANVTGSYGAACN 317

[Close]
BLAST of Moc10g02660 vs. ExPASy TrEMBL
Match: A0A6J1CI32 (probable isoaspartyl peptidase/L-asparaginase 3 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111011702 PE=4 SV=1)

HSP 1 Score: 745.7 bits (1924), Expect = 8.8e-212
Identity = 361/361 (100.00%), Postives = 361/361 (100.00%), Query Frame = 0 

Query: 1   MELFPQDPNNQFRACVLLLVLLLQVTGRVSGEPGSYPLVVSTWPFLEAVERAWDAVNNGY 60
           MELFPQDPNNQFRACVLLLVLLLQVTGRVSGEPGSYPLVVSTWPFLEAVERAWDAVNNGY
Sbjct: 1   MELFPQDPNNQFRACVLLLVLLLQVTGRVSGEPGSYPLVVSTWPFLEAVERAWDAVNNGY 60

Query: 61  SAVDAVVEGCSACEELRCDGTVGPGGSPDENGETTIDAMVMDGMTMEVGAVAAMRYIKDG 120
           SAVDAVVEGCSACEELRCDGTVGPGGSPDENGETTIDAMVMDGMTMEVGAVAAMRYIKDG
Sbjct: 61  SAVDAVVEGCSACEELRCDGTVGPGGSPDENGETTIDAMVMDGMTMEVGAVAAMRYIKDG 120

Query: 121 IRAARLVMRHTKHTLLVGEKASAFSLSMGLPGPTNLSSPESIEKWNTWKENNCQPNFWKN 180
           IRAARLVMRHTKHTLLVGEKASAFSLSMGLPGPTNLSSPESIEKWNTWKENNCQPNFWKN
Sbjct: 121 IRAARLVMRHTKHTLLVGEKASAFSLSMGLPGPTNLSSPESIEKWNTWKENNCQPNFWKN 180

Query: 181 VVPLNSCGPYYYEDPLVVTGKMCRGEDQRGTVELRSDHVGLHSHDTISMAVIDKFGHIAV 240
           VVPLNSCGPYYYEDPLVVTGKMCRGEDQRGTVELRSDHVGLHSHDTISMAVIDKFGHIAV
Sbjct: 181 VVPLNSCGPYYYEDPLVVTGKMCRGEDQRGTVELRSDHVGLHSHDTISMAVIDKFGHIAV 240

Query: 241 GTSTNGATFKIPGRVGDGPIAGSSAYADGDIGACGATGDGDIMMRFLPCYQVVESMRLGM 300
           GTSTNGATFKIPGRVGDGPIAGSSAYADGDIGACGATGDGDIMMRFLPCYQVVESMRLGM
Sbjct: 241 GTSTNGATFKIPGRVGDGPIAGSSAYADGDIGACGATGDGDIMMRFLPCYQVVESMRLGM 300

Query: 301 EPKDAAKDAISRITRKFPDFVGAIFAVNKTGTHAGACHGWTFQYSVRNAEMLRAEVFTVL 360
           EPKDAAKDAISRITRKFPDFVGAIFAVNKTGTHAGACHGWTFQYSVRNAEMLRAEVFTVL
Sbjct: 301 EPKDAAKDAISRITRKFPDFVGAIFAVNKTGTHAGACHGWTFQYSVRNAEMLRAEVFTVL 360

Query: 361 P 362
           P
Sbjct: 361 P 361

[Close]
BLAST of Moc10g02660 vs. ExPASy TrEMBL
Match: A0A6J1JDF2 (probable isoaspartyl peptidase/L-asparaginase 3 OS=Cucurbita maxima OX=3661 GN=LOC111483481 PE=4 SV=1)

HSP 1 Score: 646.0 bits (1665), Expect = 9.5e-182
Identity = 313/361 (86.70%), Postives = 330/361 (91.41%), Query Frame = 0 

Query: 1   MELFPQDPNNQFRACVLLLVLLLQVTGRVSGEPGSYPLVVSTWPFLEAVERAWDAVNNGY 60
           MEL+  D NN+FRA  LLLVLL QVTGRV+GE GSYPLVVSTWPFLEAVERAW AVNNG 
Sbjct: 1   MELYAHDSNNRFRASALLLVLLFQVTGRVAGESGSYPLVVSTWPFLEAVERAWSAVNNGS 60

Query: 61  SAVDAVVEGCSACEELRCDGTVGPGGSPDENGETTIDAMVMDGMTMEVGAVAAMRYIKDG 120
           SAVDAVV+GCSACEELRCDGTVGPGGSPDENGETTIDAMVMDG+TMEVGAVAAMRYIKDG
Sbjct: 61  SAVDAVVDGCSACEELRCDGTVGPGGSPDENGETTIDAMVMDGVTMEVGAVAAMRYIKDG 120

Query: 121 IRAARLVMRHTKHTLLVGEKASAFSLSMGLPGPTNLSSPESIEKWNTWKENNCQPNFWKN 180
           I+AARLVMRHT+HTLLVGEKASAFS+SMGLPGP +LSS ESIEKWN WKENNCQPNFWKN
Sbjct: 121 IKAARLVMRHTQHTLLVGEKASAFSISMGLPGPEDLSSSESIEKWNKWKENNCQPNFWKN 180

Query: 181 VVPLNSCGPYYYEDPLVVTGKMCRGEDQRGTVELRSDHVGLHSHDTISMAVIDKFGHIAV 240
           VVP+NSCGPYY  D +VV    C G+D   TV LRS+H GLHSHDTISMAVIDKFGHIAV
Sbjct: 181 VVPVNSCGPYYSNDLMVVAETTCSGDDPMRTVGLRSNHFGLHSHDTISMAVIDKFGHIAV 240

Query: 241 GTSTNGATFKIPGRVGDGPIAGSSAYADGDIGACGATGDGDIMMRFLPCYQVVESMRLGM 300
           GTSTNGATFKIPGRVGDGPIAGSSAYAD DIGACGATGDGDIMMRFLPCYQVVESMRLGM
Sbjct: 241 GTSTNGATFKIPGRVGDGPIAGSSAYADSDIGACGATGDGDIMMRFLPCYQVVESMRLGM 300

Query: 301 EPKDAAKDAISRITRKFPDFVGAIFAVNKTGTHAGACHGWTFQYSVRNAEMLRAEVFTVL 360
           +PKDAAKDAISRI RKFPDF+GAIFAVNK GTHAGACHGWTF+YSVR+ EM  AEVFTVL
Sbjct: 301 DPKDAAKDAISRIARKFPDFIGAIFAVNKNGTHAGACHGWTFKYSVRSPEMHHAEVFTVL 360

Query: 361 P 362
           P
Sbjct: 361 P 361

[Close]
BLAST of Moc10g02660 vs. ExPASy TrEMBL
Match: A0A6J1E462 (probable isoaspartyl peptidase/L-asparaginase 3 OS=Cucurbita moschata OX=3662 GN=LOC111430645 PE=4 SV=1)

HSP 1 Score: 636.7 bits (1641), Expect = 5.8e-179
Identity = 309/353 (87.54%), Postives = 324/353 (91.78%), Query Frame = 0 

Query: 9   NNQFRACVLLLVLLLQVTGRVSGEPGSYPLVVSTWPFLEAVERAWDAVNNGYSAVDAVVE 68
           NN+FRA  LLLVLL QVTGRV+GE GSYPLVVSTWPF EAVERAW AVNNG SAVDAVVE
Sbjct: 7   NNRFRASALLLVLLFQVTGRVAGESGSYPLVVSTWPFFEAVERAWSAVNNGSSAVDAVVE 66

Query: 69  GCSACEELRCDGTVGPGGSPDENGETTIDAMVMDGMTMEVGAVAAMRYIKDGIRAARLVM 128
           GCSACEELRCDGTVGPGGSPDENGETTIDAMVMDG+TMEVGAVAAMRYIKDGI+AARLVM
Sbjct: 67  GCSACEELRCDGTVGPGGSPDENGETTIDAMVMDGVTMEVGAVAAMRYIKDGIKAARLVM 126

Query: 129 RHTKHTLLVGEKASAFSLSMGLPGPTNLSSPESIEKWNTWKENNCQPNFWKNVVPLNSCG 188
           RHT+HTLLVGEKASAFS+SMGLPGP +LSS ESIEKWN WKENNCQPNFWKNVVP+NSCG
Sbjct: 127 RHTQHTLLVGEKASAFSISMGLPGPEDLSSSESIEKWNKWKENNCQPNFWKNVVPVNSCG 186

Query: 189 PYYYEDPLVVTGKMCRGEDQRGTVELRSDHVGLHSHDTISMAVIDKFGHIAVGTSTNGAT 248
           PY+  D ++V    C G+D   TV LRS+H GLHSHDTISMAVIDKFGHIAVGTSTNGAT
Sbjct: 187 PYHSNDLMLVAETTCSGDDPMRTVGLRSNHFGLHSHDTISMAVIDKFGHIAVGTSTNGAT 246

Query: 249 FKIPGRVGDGPIAGSSAYADGDIGACGATGDGDIMMRFLPCYQVVESMRLGMEPKDAAKD 308
           FKIPGRVGDGPIAGSSAYAD DIGACGATGDGDIMMRFLPCYQVVESMRLGMEPKDAAKD
Sbjct: 247 FKIPGRVGDGPIAGSSAYADSDIGACGATGDGDIMMRFLPCYQVVESMRLGMEPKDAAKD 306

Query: 309 AISRITRKFPDFVGAIFAVNKTGTHAGACHGWTFQYSVRNAEMLRAEVFTVLP 362
           AISRI RKFPDF+GAIFAVNK GTHAGACHGWTFQYSVR+ EM  AEVFTVLP
Sbjct: 307 AISRIARKFPDFIGAIFAVNKNGTHAGACHGWTFQYSVRSPEMHHAEVFTVLP 359

[Close]
BLAST of Moc10g02660 vs. ExPASy TrEMBL
Match: A0A1S3BWS8 (probable isoaspartyl peptidase/L-asparaginase 3 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103494471 PE=4 SV=1)

HSP 1 Score: 622.9 bits (1605), Expect = 8.6e-175
Identity = 304/361 (84.21%), Postives = 319/361 (88.37%), Query Frame = 0 

Query: 1   MELFPQDPNNQFRACVLLLVLLLQVTGRVSGEPGSYPLVVSTWPFLEAVERAWDAVNNGY 60
           ME F  D  ++ RA  LLL+LL QVT RV+G    YPLVVSTWPFLEAVERAW A NNGY
Sbjct: 1   MERFGLDSKDRLRASALLLILLFQVTERVAGASSLYPLVVSTWPFLEAVERAWSAANNGY 60

Query: 61  SAVDAVVEGCSACEELRCDGTVGPGGSPDENGETTIDAMVMDGMTMEVGAVAAMRYIKDG 120
           SAVD+VVEGCSACEELRCDGTVGPGGSPDENGETTIDAMVMDG+TMEVGAVAAMRYIKDG
Sbjct: 61  SAVDSVVEGCSACEELRCDGTVGPGGSPDENGETTIDAMVMDGVTMEVGAVAAMRYIKDG 120

Query: 121 IRAARLVMRHTKHTLLVGEKASAFSLSMGLPGPTNLSSPESIEKWNTWKENNCQPNFWKN 180
           I+AARLVMRHT HTLLVGEKASAFS+SMGLPGPT+LSSPESIEKWN WKENNCQPNFWKN
Sbjct: 121 IKAARLVMRHTGHTLLVGEKASAFSISMGLPGPTDLSSPESIEKWNKWKENNCQPNFWKN 180

Query: 181 VVPLNSCGPYYYEDPLVVTGKMCRGEDQRGTVELRSDHVGLHSHDTISMAVIDKFGHIAV 240
           VVP NSCGPY+    L+     C G+  R  VELRS+H GLHSHDTISMAVIDKFGHIAV
Sbjct: 181 VVPANSCGPYHSNGLLLAAETTCLGDGPRRAVELRSNHFGLHSHDTISMAVIDKFGHIAV 240

Query: 241 GTSTNGATFKIPGRVGDGPIAGSSAYADGDIGACGATGDGDIMMRFLPCYQVVESMRLGM 300
           GTSTNGATFKIPGRVGDGPIAGSSAY D DIGACGATGDGDIMMRFLPCYQVVESMRLGM
Sbjct: 241 GTSTNGATFKIPGRVGDGPIAGSSAYVDSDIGACGATGDGDIMMRFLPCYQVVESMRLGM 300

Query: 301 EPKDAAKDAISRITRKFPDFVGAIFAVNKTGTHAGACHGWTFQYSVRNAEMLRAEVFTVL 360
            PKDAAKDAISRI RKFPDFVGAIFAVNK GTHAGACHGWTFQYSVR+ EM  A+VFTVL
Sbjct: 301 TPKDAAKDAISRIARKFPDFVGAIFAVNKNGTHAGACHGWTFQYSVRSPEMHSAKVFTVL 360

Query: 361 P 362
           P
Sbjct: 361 P 361

[Close]
BLAST of Moc10g02660 vs. ExPASy TrEMBL
Match: A0A5A7TRA8 (Putative isoaspartyl peptidase/L-asparaginase 3 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold46G004220 PE=4 SV=1)

HSP 1 Score: 622.5 bits (1604), Expect = 1.1e-174
Identity = 304/361 (84.21%), Postives = 319/361 (88.37%), Query Frame = 0 

Query: 1   MELFPQDPNNQFRACVLLLVLLLQVTGRVSGEPGSYPLVVSTWPFLEAVERAWDAVNNGY 60
           ME F  D  ++ RA  LLL+LL QVT RV+G    YPLVVSTWPFLEAVERAW A NNGY
Sbjct: 1   MERFGLDSKDRLRASALLLILLFQVTERVAGASSLYPLVVSTWPFLEAVERAWSAANNGY 60

Query: 61  SAVDAVVEGCSACEELRCDGTVGPGGSPDENGETTIDAMVMDGMTMEVGAVAAMRYIKDG 120
           SAVD+VVEGCSACEELRCDGTVGPGGSPDENGETTIDAMVMDG+TMEVGAVAAMRYIKDG
Sbjct: 61  SAVDSVVEGCSACEELRCDGTVGPGGSPDENGETTIDAMVMDGVTMEVGAVAAMRYIKDG 120

Query: 121 IRAARLVMRHTKHTLLVGEKASAFSLSMGLPGPTNLSSPESIEKWNTWKENNCQPNFWKN 180
           I+AARLVMRHT HTLLVGEKASAFS+SMGLPGPT+LSSPESIEKWN WKENNCQPNFWKN
Sbjct: 121 IKAARLVMRHTGHTLLVGEKASAFSISMGLPGPTDLSSPESIEKWNKWKENNCQPNFWKN 180

Query: 181 VVPLNSCGPYYYEDPLVVTGKMCRGEDQRGTVELRSDHVGLHSHDTISMAVIDKFGHIAV 240
           VVP NSCGPY+    L+     C G+  R  VELRS+H GLHSHDTISMAVIDKFGHIAV
Sbjct: 181 VVPANSCGPYHSNGLLLAAETTCLGDGPRRAVELRSNHFGLHSHDTISMAVIDKFGHIAV 240

Query: 241 GTSTNGATFKIPGRVGDGPIAGSSAYADGDIGACGATGDGDIMMRFLPCYQVVESMRLGM 300
           GTSTNGATFKIPGRVGDGPIAGSSAY D DIGACGATGDGDIMMRFLPCYQVVESMRLGM
Sbjct: 241 GTSTNGATFKIPGRVGDGPIAGSSAYVDSDIGACGATGDGDIMMRFLPCYQVVESMRLGM 300

Query: 301 EPKDAAKDAISRITRKFPDFVGAIFAVNKTGTHAGACHGWTFQYSVRNAEMLRAEVFTVL 360
            PKDAAKDAISRI RKFPDFVGAIFAVNK GTHAGACHGWTFQYSVR+ EM  A+VFTVL
Sbjct: 301 MPKDAAKDAISRIARKFPDFVGAIFAVNKNGTHAGACHGWTFQYSVRSPEMHSAKVFTVL 360

Query: 361 P 362
           P
Sbjct: 361 P 361

[Close]
BLAST of Moc10g02660 vs. TAIR 10
Match: AT5G61540.1 (N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein )

HSP 1 Score: 508.4 bits (1308), Expect = 4.6e-144
Identity = 248/351 (70.66%), Postives = 288/351 (82.05%), Query Frame = 0 

Query: 12  FRACVLLLVLLLQVTGRVSGEPGSYPLVVSTWPFLEAVERAWDAVNNGYSAVDAVVEGCS 71
           F + +LL + LL V      +   +P+VVSTWPFLEAV  AW AV+NG SAV+AVVEGCS
Sbjct: 9   FVSTLLLFLSLLTVADAELVKSDKFPVVVSTWPFLEAVRAAWRAVDNGSSAVEAVVEGCS 68

Query: 72  ACEELRCDGTVGPGGSPDENGETTIDAMVMDGMTMEVGAVAAMRYIKDGIRAARLVMRHT 131
           ACEELRCDGTVGPGGSPDENGET IDA+VMDG+TMEVGAVAAMRY+KDGIRAA LVM+++
Sbjct: 69  ACEELRCDGTVGPGGSPDENGETMIDALVMDGVTMEVGAVAAMRYVKDGIRAAHLVMKYS 128

Query: 132 KHTLLVGEKASAFSLSMGLPGPTNLSSPESIEKWNTWKENNCQPNFWKNVVPLNSCGPYY 191
           +HTLL GE ASAF++SMGLPGP NLSSPES++KW+ WKEN CQPNF KNVVP N CGPY 
Sbjct: 129 QHTLLAGEGASAFAISMGLPGPMNLSSPESVKKWSDWKENQCQPNFRKNVVPANDCGPYK 188

Query: 192 YEDPLV-VTGKMCRGEDQRGTVELRSDHVGLHSHDTISMAVIDKFGHIAVGTSTNGATFK 251
             +  + V         + G +E +   VG H+HDTISMAVID+ GHIAVGTSTNGAT+K
Sbjct: 189 PNNSAMNVFVDKSTESCEMGAIEYKPPLVGPHNHDTISMAVIDRMGHIAVGTSTNGATYK 248

Query: 252 IPGRVGDGPIAGSSAYADGDIGACGATGDGDIMMRFLPCYQVVESMRLGMEPKDAAKDAI 311
           IPGRVGDGPI GSSAYAD ++G CGATGDGD MMRFLPCYQVVESMR GM+P++AAKDAI
Sbjct: 249 IPGRVGDGPIVGSSAYADDEVGGCGATGDGDTMMRFLPCYQVVESMRQGMKPEEAAKDAI 308

Query: 312 SRITRKFPDFVGAIFAVNKTGTHAGACHGWTFQYSVRNAEMLRAEVFTVLP 362
           SRI RKFPDFVGA+ AV+K G+HAGAC+GWTFQYSV+N +M   +VFTVLP
Sbjct: 309 SRIARKFPDFVGAVVAVDKNGSHAGACYGWTFQYSVQNPDMNDVQVFTVLP 359

[Close]
BLAST of Moc10g02660 vs. TAIR 10
Match: AT5G61540.2 (N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein )

HSP 1 Score: 379.8 bits (974), Expect = 2.5e-105
Identity = 183/257 (71.21%), Postives = 213/257 (82.88%), Query Frame = 0 

Query: 106 MEVGAVAAMRYIKDGIRAARLVMRHTKHTLLVGEKASAFSLSMGLPGPTNLSSPESIEKW 165
           MEVGAVAAMRY+KDGIRAA LVM++++HTLL GE ASAF++SMGLPGP NLSSPES++KW
Sbjct: 1   MEVGAVAAMRYVKDGIRAAHLVMKYSQHTLLAGEGASAFAISMGLPGPMNLSSPESVKKW 60

Query: 166 NTWKENNCQPNFWKNVVPLNSCGPYYYEDPLV-VTGKMCRGEDQRGTVELRSDHVGLHSH 225
           + WKEN CQPNF KNVVP N CGPY   +  + V         + G +E +   VG H+H
Sbjct: 61  SDWKENQCQPNFRKNVVPANDCGPYKPNNSAMNVFVDKSTESCEMGAIEYKPPLVGPHNH 120

Query: 226 DTISMAVIDKFGHIAVGTSTNGATFKIPGRVGDGPIAGSSAYADGDIGACGATGDGDIMM 285
           DTISMAVID+ GHIAVGTSTNGAT+KIPGRVGDGPI GSSAYAD ++G CGATGDGD MM
Sbjct: 121 DTISMAVIDRMGHIAVGTSTNGATYKIPGRVGDGPIVGSSAYADDEVGGCGATGDGDTMM 180

Query: 286 RFLPCYQVVESMRLGMEPKDAAKDAISRITRKFPDFVGAIFAVNKTGTHAGACHGWTFQY 345
           RFLPCYQVVESMR GM+P++AAKDAISRI RKFPDFVGA+ AV+K G+HAGAC+GWTFQY
Sbjct: 181 RFLPCYQVVESMRQGMKPEEAAKDAISRIARKFPDFVGAVVAVDKNGSHAGACYGWTFQY 240

Query: 346 SVRNAEMLRAEVFTVLP 362
           SV+N +M   +VFTVLP
Sbjct: 241 SVQNPDMNDVQVFTVLP 257

[Close]
BLAST of Moc10g02660 vs. TAIR 10
Match: AT5G61540.3 (N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein )

HSP 1 Score: 379.8 bits (974), Expect = 2.5e-105
Identity = 183/257 (71.21%), Postives = 213/257 (82.88%), Query Frame = 0 

Query: 106 MEVGAVAAMRYIKDGIRAARLVMRHTKHTLLVGEKASAFSLSMGLPGPTNLSSPESIEKW 165
           MEVGAVAAMRY+KDGIRAA LVM++++HTLL GE ASAF++SMGLPGP NLSSPES++KW
Sbjct: 1   MEVGAVAAMRYVKDGIRAAHLVMKYSQHTLLAGEGASAFAISMGLPGPMNLSSPESVKKW 60

Query: 166 NTWKENNCQPNFWKNVVPLNSCGPYYYEDPLV-VTGKMCRGEDQRGTVELRSDHVGLHSH 225
           + WKEN CQPNF KNVVP N CGPY   +  + V         + G +E +   VG H+H
Sbjct: 61  SDWKENQCQPNFRKNVVPANDCGPYKPNNSAMNVFVDKSTESCEMGAIEYKPPLVGPHNH 120

Query: 226 DTISMAVIDKFGHIAVGTSTNGATFKIPGRVGDGPIAGSSAYADGDIGACGATGDGDIMM 285
           DTISMAVID+ GHIAVGTSTNGAT+KIPGRVGDGPI GSSAYAD ++G CGATGDGD MM
Sbjct: 121 DTISMAVIDRMGHIAVGTSTNGATYKIPGRVGDGPIVGSSAYADDEVGGCGATGDGDTMM 180

Query: 286 RFLPCYQVVESMRLGMEPKDAAKDAISRITRKFPDFVGAIFAVNKTGTHAGACHGWTFQY 345
           RFLPCYQVVESMR GM+P++AAKDAISRI RKFPDFVGA+ AV+K G+HAGAC+GWTFQY
Sbjct: 181 RFLPCYQVVESMRQGMKPEEAAKDAISRIARKFPDFVGAVVAVDKNGSHAGACYGWTFQY 240

Query: 346 SVRNAEMLRAEVFTVLP 362
           SV+N +M   +VFTVLP
Sbjct: 241 SVQNPDMNDVQVFTVLP 257

[Close]
BLAST of Moc10g02660 vs. TAIR 10
Match: AT3G16150.1 (N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein )

HSP 1 Score: 80.9 bits (198), Expect = 2.4e-15
Identity = 78/280 (27.86%), Postives = 120/280 (42.86%), Query Frame = 0 

Query: 55  AVNNGYSAVDAVVEGCSACEELRCDG--TVGPGGSPDENGETTIDAMVMDGMTMEVGAVA 114
           A+ +  SA+D VVE      EL  D     G G +  E G   ++A +MDG     GAV+
Sbjct: 40  ALRSNVSAID-VVE--LVIRELETDPLFNSGRGSALTEKGTVEMEASIMDGTKRRCGAVS 99

Query: 115 AMRYIKDGIRAARLVMRHTKHTLLVGEKASAFSLSMGLPGPTN--LSSPESIEKWNTWKE 174
            +  +K+ I  ARLVM  + H+ L    A  F+   G+    N    + +++      KE
Sbjct: 100 GITTVKNPISLARLVMDKSPHSYLAFSGAEDFARKQGVEIVDNEYFVTDDNVGMLKLAKE 159

Query: 175 NNCQPNFWKNVVPLNSCGPYYYEDPLVVTGKMCRGEDQRGTVELRSDHVGLHSHDTISMA 234
            N    F   + P+   G    + P+ + G                  + +++ +T+   
Sbjct: 160 AN-SILFDYRIPPMGCAGAAATDSPIQMNGL----------------PISIYAPETVGCV 219

Query: 235 VIDKFGHIAVGTSTNGATFKIPGRVGDGPIAGSSAYADGDIGACGATGDGDIMMRFLPCY 294
           V+D  GH A GTST G   K+ GR+GD P+ G+  YA    G    TG+G+ ++R     
Sbjct: 220 VVDGKGHCAAGTSTGGLMNKMMGRIGDSPLIGAGTYASEFCGV-SCTGEGEAIIRATLAR 279

Query: 295 QVVESMRL-GMEPKDAAKDAISRITRKFPDFVGAIFAVNK 330
            V   M   G+  ++A    I    R    F G I   NK
Sbjct: 280 DVSAVMEYKGLNLQEAVDYVIKH--RLDEGFAGLIAVSNK 296

The following BLAST results are available for this feature:
BLAST of Moc10g02660 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_022141259.11.8e-211100.00probable isoaspartyl peptidase/L-asparaginase 3 isoform X1 [Momordica charantia][more]
XP_022985489.12.0e-18186.70probable isoaspartyl peptidase/L-asparaginase 3 [Cucurbita maxima][more]
XP_038900285.14.8e-18085.87probable isoaspartyl peptidase/L-asparaginase 3 isoform X1 [Benincasa hispida][more]
KAG6576819.11.2e-17887.54putative isoaspartyl peptidase/L-asparaginase 3, partial [Cucurbita argyrosperma... [more]
XP_022922742.11.2e-17887.54probable isoaspartyl peptidase/L-asparaginase 3 [Cucurbita moschata][more]
BLAST of Moc10g02660 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q56W646.4e-14370.66Probable isoaspartyl peptidase/L-asparaginase 3 OS=Arabidopsis thaliana OX=3702 ... [more]
P209335.4e-8150.29N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase OS=Homo sapiens OX=9606 GN=AGA P... [more]
Q641917.0e-8150.00N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase OS=Mus musculus OX=10090 GN=Aga ... [more]
B4NWI11.6e-8052.75Putative N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase GE19290 OS=Drosophila y... [more]
P309192.0e-8050.62N(4)-(Beta-N-acetylglucosaminyl)-L-asparaginase OS=Rattus norvegicus OX=10116 GN... [more]
BLAST of Moc10g02660 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1CI328.8e-212100.00probable isoaspartyl peptidase/L-asparaginase 3 isoform X1 OS=Momordica charanti... [more]
A0A6J1JDF29.5e-18286.70probable isoaspartyl peptidase/L-asparaginase 3 OS=Cucurbita maxima OX=3661 GN=L... [more]
A0A6J1E4625.8e-17987.54probable isoaspartyl peptidase/L-asparaginase 3 OS=Cucurbita moschata OX=3662 GN... [more]
A0A1S3BWS88.6e-17584.21probable isoaspartyl peptidase/L-asparaginase 3 isoform X1 OS=Cucumis melo OX=36... [more]
A0A5A7TRA81.1e-17484.21Putative isoaspartyl peptidase/L-asparaginase 3 isoform X1 OS=Cucumis melo var. ... [more]
BLAST of Moc10g02660 vs. TAIR 10
Match NameE-valueIdentityDescription
AT5G61540.14.6e-14470.66N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein [more]
AT5G61540.22.5e-10571.21N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein [more]
AT5G61540.32.5e-10571.21N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein [more]
AT3G16150.12.4e-1527.86N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.60.20.30(Glycosyl)asparaginasecoord: 226..359
e-value: 3.4E-40
score: 139.5
NoneNo IPR availablePIRSRPIRSR600246-1PIRSR600246-1coord: 38..338
e-value: 1.4E-81
score: 271.9
NoneNo IPR availablePIRSRPIRSR600246-2PIRSR600246-2coord: 38..338
e-value: 1.4E-81
score: 271.9
NoneNo IPR availablePIRSRPIRSR600246-3PIRSR600246-3coord: 38..338
e-value: 1.4E-81
score: 271.9
NoneNo IPR availablePANTHERPTHR10188:SF6N(4)-(BETA-N-ACETYLGLUCOSAMINYL)-L-ASPARAGINASEcoord: 17..359
NoneNo IPR availableCDDcd04513Glycosylasparaginasecoord: 38..348
e-value: 5.51087E-171
score: 475.898
IPR000246Peptidase T2, asparaginase 2PFAMPF01112Asparaginase_2coord: 46..348
e-value: 3.1E-75
score: 253.1
IPR000246Peptidase T2, asparaginase 2PANTHERPTHR10188L-ASPARAGINASEcoord: 17..359
IPR029055Nucleophile aminohydrolases, N-terminalSUPERFAMILY56235N-terminal nucleophile aminohydrolases (Ntn hydrolases)coord: 36..354

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc10g02660.1Moc10g02660.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006517 protein deglycosylation
cellular_component GO:0005737 cytoplasm
molecular_function GO:0003948 N4-(beta-N-acetylglucosaminyl)-L-asparaginase activity
molecular_function GO:0016787 hydrolase activity