Moc10g02350 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc10g02350
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	Pectinesterase
Location	chr10: 1508873 .. 1510643 (+)
RNA-Seq Expression	Moc10g02350
Synteny	Moc10g02350

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGATACAAACGGCCCTGGAAAAAGCCGCCGAGGCCCAACATTACGCGACCCGGTTCGGCCAGAGCTGTAAGAACACGCGCCAGAGAGCCGCGTGGGCCGACTGCTTGAAGCTGTACCACGACGCCGTTTTTCAGTTGAACCGCACTCTCCAGGGCCTCAGCCGCAAAGGGTGTACGGATTTCGACGCGCAGACGTGGCTGAGCTCTGCCCTCACAAGCATCGACACGTGTCTTTCCGGCGCGGCGGATCTCAATGTGACGGATTTTATAGCGCCGATCAGATTCCAGAATGTTTCGGAGATGATTAGCAATTGTTTGGCCATTAATGGGGCGTTTTTGGGGGATGGAAAAAATGGTACGGAGGAGGAAAATGGGTTTCCGATGTGGGTTTCGAGTGGTGATCGGAAATTGTTGCAGTCGTCGTCGACGAAGGTGAGGGCTAATTTGGTGGTGGCGAGAGATGGGTCGGGGAATTTCCGGACGGTTCAGGCGGCCATCAACGCCGCCGCGAGGAGGCGTGGGAGGGGTCGATTTGTAATTTACGTAAAGCGAGGGGTTTATAGAGAAAATATTGAAGTTGAGAATGATAATGGTAATTTAATGCTGGTCGGAGATGGGATGAGATTTACTGTCATTACAAGTGGGAGAAGCGTCGCCGCCGGCTTCACCACCTTCAGTTCAGCAACCGCCGGTGAGAGCTAAATGTATTTTTTAATCTAGATTTTTTTTTCCAATCTAGTCTCCATTTCTGAAAATGTTTTTCATTTATTATATATTTTTAACATATAGCATAATTGAGTTTTTTTTTTTTTTTTGTCATGCATGAATTATGAAAAATACTTAATTTCTTACATTGAGAACTTATTATTTTTTTTTAAAAAAAAGATATTTTTGCATTGTAAATTTAATTCAACCACATTTAACCTCTAGAAGGGTATATAATTAAAAAATGTAAATACGTATGGACAAAAAAATGAAATAACGTATTCAAACCATAAAAATTAAAACATATATTTGGTGTTTTGAGATGCGGCTTATGATCTTGATCTCCTTTTATTTTTATGAATTTCTAGGTATTGAAGGGCCTGCTTTCATAGCTCGCGACATTACCTTCGTCAACACCGCGGGCCCCCGGATGGGCCAAGCCGTTGCGCTCCGGTCATCGTCCGATCTATCGGTGTTCCACCGGTGCGGCTTTCAGGGCTACCAAGACACTCTAATGGTGCTCTCACAGAGACAATTCTACAAGCAATGTTATGTGTACGGAACAATTGACTTCATTTTTGGGAACGCAGCAGTTGTTTTACAAAACTGCATGATTTACGTACGAAGGCCCTTAAAGGGCCAGGTCAACGTCATCACGGCCCAAGGCCGAGAGGACCCGTATCAAAACAGCGGAATCTCGATCCACAATTCTCAAATACGGGCCGCTCCGGATTTGAGGCCCGTTATTGGGTCGGTCAAAACTTACTTGGGCCGGCCCTGGAAGAAGTATTCGCGGACCGTGATCATGAGGAGCTATATTGACAGCCTGGTGAGCCCAATGGGCTGGCTCGCGTGGCAAAGTAGTAAATTTGCTCAGGATACGCTGTATTATGGGGAATTTAAGAACTTTGGGCCTCGGGCTTCGACCCGGTATAGAGTGAGATGGCCTGGTTTTCATGTTATAAGGAGCCCAAATGTGGCCTCTCAGTTCACCGTCCGGCGACTCATCGCCGGCCAGACCTGGTTGCCGGCCACCGGAGTTCCCTTCACCCTTGGCCTCTGA

mRNA sequence

ATGGCGATACAAACGGCCCTGGAAAAAGCCGCCGAGGCCCAACATTACGCGACCCGGTTCGGCCAGAGCTGTAAGAACACGCGCCAGAGAGCCGCGTGGGCCGACTGCTTGAAGCTGTACCACGACGCCGTTTTTCAGTTGAACCGCACTCTCCAGGGCCTCAGCCGCAAAGGGTGTACGGATTTCGACGCGCAGACGTGGCTGAGCTCTGCCCTCACAAGCATCGACACGTGTCTTTCCGGCGCGGCGGATCTCAATGTGACGGATTTTATAGCGCCGATCAGATTCCAGAATGTTTCGGAGATGATTAGCAATTGTTTGGCCATTAATGGGGCGTTTTTGGGGGATGGAAAAAATGGTACGGAGGAGGAAAATGGGTTTCCGATGTGGGTTTCGAGTGGTGATCGGAAATTGTTGCAGTCGTCGTCGACGAAGGTGAGGGCTAATTTGGTGGTGGCGAGAGATGGGTCGGGGAATTTCCGGACGGTTCAGGCGGCCATCAACGCCGCCGCGAGGAGGCGTGGGAGGGGTCGATTTGTAATTTACGTAAAGCGAGGGGTTTATAGAGAAAATATTGAAGTTGAGAATGATAATGGTAATTTAATGCTGGTCGGAGATGGGATGAGATTTACTGTCATTACAAGTGGGAGAAGCGTCGCCGCCGGCTTCACCACCTTCAGTTCAGCAACCGCCGGTATTGAAGGGCCTGCTTTCATAGCTCGCGACATTACCTTCGTCAACACCGCGGGCCCCCGGATGGGCCAAGCCGTTGCGCTCCGGTCATCGTCCGATCTATCGGTGTTCCACCGGTGCGGCTTTCAGGGCTACCAAGACACTCTAATGGTGCTCTCACAGAGACAATTCTACAAGCAATGTTATGTGTACGGAACAATTGACTTCATTTTTGGGAACGCAGCAGTTGTTTTACAAAACTGCATGATTTACGTACGAAGGCCCTTAAAGGGCCAGGTCAACGTCATCACGGCCCAAGGCCGAGAGGACCCGTATCAAAACAGCGGAATCTCGATCCACAATTCTCAAATACGGGCCGCTCCGGATTTGAGGCCCGTTATTGGGTCGGTCAAAACTTACTTGGGCCGGCCCTGGAAGAAGTATTCGCGGACCGTGATCATGAGGAGCTATATTGACAGCCTGGTGAGCCCAATGGGCTGGCTCGCGTGGCAAAGTAGTAAATTTGCTCAGGATACGCTGTATTATGGGGAATTTAAGAACTTTGGGCCTCGGGCTTCGACCCGGTATAGAGTGAGATGGCCTGGTTTTCATGTTATAAGGAGCCCAAATGTGGCCTCTCAGTTCACCGTCCGGCGACTCATCGCCGGCCAGACCTGGTTGCCGGCCACCGGAGTTCCCTTCACCCTTGGCCTCTGA

Coding sequence (CDS)

ATGGCGATACAAACGGCCCTGGAAAAAGCCGCCGAGGCCCAACATTACGCGACCCGGTTCGGCCAGAGCTGTAAGAACACGCGCCAGAGAGCCGCGTGGGCCGACTGCTTGAAGCTGTACCACGACGCCGTTTTTCAGTTGAACCGCACTCTCCAGGGCCTCAGCCGCAAAGGGTGTACGGATTTCGACGCGCAGACGTGGCTGAGCTCTGCCCTCACAAGCATCGACACGTGTCTTTCCGGCGCGGCGGATCTCAATGTGACGGATTTTATAGCGCCGATCAGATTCCAGAATGTTTCGGAGATGATTAGCAATTGTTTGGCCATTAATGGGGCGTTTTTGGGGGATGGAAAAAATGGTACGGAGGAGGAAAATGGGTTTCCGATGTGGGTTTCGAGTGGTGATCGGAAATTGTTGCAGTCGTCGTCGACGAAGGTGAGGGCTAATTTGGTGGTGGCGAGAGATGGGTCGGGGAATTTCCGGACGGTTCAGGCGGCCATCAACGCCGCCGCGAGGAGGCGTGGGAGGGGTCGATTTGTAATTTACGTAAAGCGAGGGGTTTATAGAGAAAATATTGAAGTTGAGAATGATAATGGTAATTTAATGCTGGTCGGAGATGGGATGAGATTTACTGTCATTACAAGTGGGAGAAGCGTCGCCGCCGGCTTCACCACCTTCAGTTCAGCAACCGCCGGTATTGAAGGGCCTGCTTTCATAGCTCGCGACATTACCTTCGTCAACACCGCGGGCCCCCGGATGGGCCAAGCCGTTGCGCTCCGGTCATCGTCCGATCTATCGGTGTTCCACCGGTGCGGCTTTCAGGGCTACCAAGACACTCTAATGGTGCTCTCACAGAGACAATTCTACAAGCAATGTTATGTGTACGGAACAATTGACTTCATTTTTGGGAACGCAGCAGTTGTTTTACAAAACTGCATGATTTACGTACGAAGGCCCTTAAAGGGCCAGGTCAACGTCATCACGGCCCAAGGCCGAGAGGACCCGTATCAAAACAGCGGAATCTCGATCCACAATTCTCAAATACGGGCCGCTCCGGATTTGAGGCCCGTTATTGGGTCGGTCAAAACTTACTTGGGCCGGCCCTGGAAGAAGTATTCGCGGACCGTGATCATGAGGAGCTATATTGACAGCCTGGTGAGCCCAATGGGCTGGCTCGCGTGGCAAAGTAGTAAATTTGCTCAGGATACGCTGTATTATGGGGAATTTAAGAACTTTGGGCCTCGGGCTTCGACCCGGTATAGAGTGAGATGGCCTGGTTTTCATGTTATAAGGAGCCCAAATGTGGCCTCTCAGTTCACCGTCCGGCGACTCATCGCCGGCCAGACCTGGTTGCCGGCCACCGGAGTTCCCTTCACCCTTGGCCTCTGA

Protein sequence

MAIQTALEKAAEAQHYATRFGQSCKNTRQRAAWADCLKLYHDAVFQLNRTLQGLSRKGCTDFDAQTWLSSALTSIDTCLSGAADLNVTDFIAPIRFQNVSEMISNCLAINGAFLGDGKNGTEEENGFPMWVSSGDRKLLQSSSTKVRANLVVARDGSGNFRTVQAAINAAARRRGRGRFVIYVKRGVYRENIEVENDNGNLMLVGDGMRFTVITSGRSVAAGFTTFSSATAGIEGPAFIARDITFVNTAGPRMGQAVALRSSSDLSVFHRCGFQGYQDTLMVLSQRQFYKQCYVYGTIDFIFGNAAVVLQNCMIYVRRPLKGQVNVITAQGREDPYQNSGISIHNSQIRAAPDLRPVIGSVKTYLGRPWKKYSRTVIMRSYIDSLVSPMGWLAWQSSKFAQDTLYYGEFKNFGPRASTRYRVRWPGFHVIRSPNVASQFTVRRLIAGQTWLPATGVPFTLGL

Homology

[Close]

BLAST of Moc10g02350 vs. NCBI nr
Match: XP_038905752.1 (probable pectinesterase/pectinesterase inhibitor 60 [Benincasa hispida])

HSP 1 Score: 789.3 bits (2037), Expect = 1.8e-224
Identity = 400/476 (84.03%), Postives = 428/476 (89.92%), Query Frame = 0


Query: 1   MAIQTALEKAAEAQHYATRFGQSCKNTRQRAAWADCLKLYHDAVFQLNRTLQGLSRKG-- 60
           MAI+TAL+KA EAQ YATRFG SCK TRQRAAW DCLKLY+D VFQLNRTLQ +   G  
Sbjct: 59  MAIRTALQKATEAQTYATRFGPSCKTTRQRAAWTDCLKLYNDVVFQLNRTLQCVISDGTI 118

Query: 61  ----CTDFDAQTWLSSALTSIDTCLSGAADLNVTDFIAPIRFQNVSEMISNCLAINGAFL 120
               CTDFDAQTWLSSALT ID C+SGAADLNVTDFIAPI+ QNVS+MISNCLA+NG FL
Sbjct: 119 RHRRCTDFDAQTWLSSALTDIDLCVSGAADLNVTDFIAPIKCQNVSKMISNCLAVNGRFL 178

Query: 121 ------GDGKNGTEEENG--FPMWVSSGDRKLLQSSSTKVRANLVVARDGSGNFRTVQAA 180
                  + K G E++    FPMWVS GDRKLLQSS+ +VRANLVVA+DGSG FRTVQAA
Sbjct: 179 EEEAKKSETKIGVEDDRDGPFPMWVSDGDRKLLQSSA-RVRANLVVAKDGSGKFRTVQAA 238

Query: 181 INAAARRRGRGRFVIYVKRGVYRENIEVENDNGNLMLVGDGMRFTVITSGRSVAAGFTTF 240
           I+AAARRRGRGRFVIYVKRGVYRENIEV NDNGNLMLVGDGMRFTVITSGRSVAAGFTTF
Sbjct: 239 IDAAARRRGRGRFVIYVKRGVYRENIEVGNDNGNLMLVGDGMRFTVITSGRSVAAGFTTF 298

Query: 241 SSATAGIEGPAFIARDITFVNTAGPRMGQAVALRSSSDLSVFHRCGFQGYQDTLMVLSQR 300
           SSATAGI+GP F+AR I FVNTAGPRMGQAVALRSSSDLSVFHRC F+GYQDTLMVLSQR
Sbjct: 299 SSATAGIQGPGFMARGIRFVNTAGPRMGQAVALRSSSDLSVFHRCSFEGYQDTLMVLSQR 358

Query: 301 QFYKQCYVYGTIDFIFGNAAVVLQNCMIYVRRPLKGQVNVITAQGREDPYQNSGISIHNS 360
           QFYKQCYVYGTIDFIFGNAAVVLQNC+IYVRRPLKGQVNVITAQGREDP+QNSGISIHNS
Sbjct: 359 QFYKQCYVYGTIDFIFGNAAVVLQNCLIYVRRPLKGQVNVITAQGREDPFQNSGISIHNS 418

Query: 361 QIRAAPDLRPVIGSVKTYLGRPWKKYSRTVIMRSYIDSLVSPMGWLAWQSSKFAQDTLYY 420
           QIRAA DLRP++GSVKTYLGRPWKKYSRTVIMRSYIDSLVSP GWLAWQSSKFAQ TLYY
Sbjct: 419 QIRAAADLRPMVGSVKTYLGRPWKKYSRTVIMRSYIDSLVSPAGWLAWQSSKFAQATLYY 478

Query: 421 GEFKNFGPRASTRYRVRWPGFHVIRSPNVASQFTVRRLIAGQTWLPATGVPFTLGL 463
           GE++N GPRA TRYRVRWPGFHVI+SPNVAS+FTV+RLIAGQTWLPATGVPF LG+
Sbjct: 479 GEYRNIGPRAFTRYRVRWPGFHVIKSPNVASKFTVQRLIAGQTWLPATGVPFKLGV 533

[Close]

BLAST of Moc10g02350 vs. NCBI nr
Match: XP_008452338.1 (PREDICTED: probable pectinesterase/pectinesterase inhibitor 59 [Cucumis melo] >XP_016901218.1 PREDICTED: probable pectinesterase/pectinesterase inhibitor 59 [Cucumis melo])

HSP 1 Score: 776.2 bits (2003), Expect = 1.6e-220
Identity = 392/476 (82.35%), Postives = 418/476 (87.82%), Query Frame = 0


Query: 1   MAIQTALEKAAEAQHYATRFGQSCKNTRQRAAWADCLKLYHDAVFQLNRTLQGL------ 60
           +AIQT LEKA EA+ Y  RFG +CK TRQR AW DC KLY+D VFQLNRTLQ L      
Sbjct: 63  VAIQTTLEKADEAKAYTARFGPTCKTTRQRTAWTDCFKLYNDVVFQLNRTLQCLVTDETI 122

Query: 61  -SRKGCTDFDAQTWLSSALTSIDTCLSGAADLNVTDFIAPIRFQNVSEMISNCLAINGAF 120
             R  CTDFDAQTWLSSALT ID C SGAADLNVTDFIAPI+  NVS+MISNCLAING F
Sbjct: 123 HRRPTCTDFDAQTWLSSALTDIDLCNSGAADLNVTDFIAPIKCLNVSKMISNCLAINGGF 182

Query: 121 L-------GDGKNGTEEENGFPMWVSSGDRKLLQSSSTKVRANLVVARDGSGNFRTVQAA 180
           L        DG NG+     FPMWVS GDRKLL+S   +VRANLVVA+DGSG FR VQAA
Sbjct: 183 LEEEGVKYDDGGNGS-----FPMWVSEGDRKLLESRPRRVRANLVVAKDGSGKFRRVQAA 242

Query: 181 INAAARRRGRGRFVIYVKRGVYRENIEVENDNGNLMLVGDGMRFTVITSGRSVAAGFTTF 240
           I+AAARRRGRGRFVIYVKRGVYRENIEV NDNGN+MLVGDGMRFTVITSGRSVAAGFTTF
Sbjct: 243 IDAAARRRGRGRFVIYVKRGVYRENIEVGNDNGNIMLVGDGMRFTVITSGRSVAAGFTTF 302

Query: 241 SSATAGIEGPAFIARDITFVNTAGPRMGQAVALRSSSDLSVFHRCGFQGYQDTLMVLSQR 300
           SSATAGI+GP FIARDI FVNTAGPRMGQAVALRSSSDLSVFHRC F+GYQDTLMVLSQR
Sbjct: 303 SSATAGIQGPGFIARDIRFVNTAGPRMGQAVALRSSSDLSVFHRCSFEGYQDTLMVLSQR 362

Query: 301 QFYKQCYVYGTIDFIFGNAAVVLQNCMIYVRRPLKGQVNVITAQGREDPYQNSGISIHNS 360
           QFYKQCY+YGTIDFIFGNAAVVLQNC+IYVRRPLKGQVNVITAQGREDP+QNSGISIHNS
Sbjct: 363 QFYKQCYIYGTIDFIFGNAAVVLQNCLIYVRRPLKGQVNVITAQGREDPFQNSGISIHNS 422

Query: 361 QIRAAPDLRPVIGSVKTYLGRPWKKYSRTVIMRSYIDSLVSPMGWLAWQSSKFAQDTLYY 420
           QIRAA DLRP++GSVKTYLGRPWKKYSRTVIMRSYIDSLVSP GWLAWQSSKFAQ TLYY
Sbjct: 423 QIRAAADLRPMVGSVKTYLGRPWKKYSRTVIMRSYIDSLVSPAGWLAWQSSKFAQATLYY 482

Query: 421 GEFKNFGPRASTRYRVRWPGFHVIRSPNVASQFTVRRLIAGQTWLPATGVPFTLGL 463
           GE+ N GPRASTR+RV+WPGFHVI+SPNVAS+F+VRRLIAGQTWLPATGVPF LG+
Sbjct: 483 GEYHNIGPRASTRFRVKWPGFHVIKSPNVASKFSVRRLIAGQTWLPATGVPFKLGV 533

[Close]

BLAST of Moc10g02350 vs. NCBI nr
Match: XP_011650542.1 (probable pectinesterase/pectinesterase inhibitor 59 [Cucumis sativus] >KGN56178.1 hypothetical protein Csa_011446 [Cucumis sativus])

HSP 1 Score: 775.4 bits (2001), Expect = 2.8e-220
Identity = 388/475 (81.68%), Postives = 419/475 (88.21%), Query Frame = 0


Query: 1   MAIQTALEKAAEAQHYATRFGQSCKNTRQRAAWADCLKLYHDAVFQLNRTL------QGL 60
           MAIQT LEKAAEA+ Y  RFG +CK +RQR AW DC KLY+D V QLNRTL      + +
Sbjct: 60  MAIQTTLEKAAEARAYTARFGPTCKTSRQRTAWTDCFKLYNDVVLQLNRTLHCVVTDEAI 119

Query: 61  SRKGCTDFDAQTWLSSALTSIDTCLSGAADLNVTDFIAPIRFQNVSEMISNCLAINGAFL 120
            R+ CTDFDAQTWLSSALT ID C SGAADLNVTDFI PI+  NVS+MISNCLAING FL
Sbjct: 120 HRRSCTDFDAQTWLSSALTDIDLCNSGAADLNVTDFITPIKCLNVSKMISNCLAINGGFL 179

Query: 121 -------GDGKNGTEEENGFPMWVSSGDRKLLQSSSTKVRANLVVARDGSGNFRTVQAAI 180
                   DG+NG+     FPMWVS GDRKLL+S   +VRANLVVA+DGSG FR VQAAI
Sbjct: 180 EEEGVKYDDGRNGS-----FPMWVSEGDRKLLESRPGRVRANLVVAKDGSGTFRRVQAAI 239

Query: 181 NAAARRRGRGRFVIYVKRGVYRENIEVENDNGNLMLVGDGMRFTVITSGRSVAAGFTTFS 240
           +AAARRRGRGRF+IYVKRGVYRENIEV NDNGN+MLVGDGMRFTVITSGRSVAAGFTTFS
Sbjct: 240 DAAARRRGRGRFIIYVKRGVYRENIEVGNDNGNIMLVGDGMRFTVITSGRSVAAGFTTFS 299

Query: 241 SATAGIEGPAFIARDITFVNTAGPRMGQAVALRSSSDLSVFHRCGFQGYQDTLMVLSQRQ 300
           SATAGI+GP FIARDI FVNTAGPRMGQAVALRSSSDLSVFHRC F+GYQDTLMVLSQRQ
Sbjct: 300 SATAGIQGPGFIARDIRFVNTAGPRMGQAVALRSSSDLSVFHRCSFEGYQDTLMVLSQRQ 359

Query: 301 FYKQCYVYGTIDFIFGNAAVVLQNCMIYVRRPLKGQVNVITAQGREDPYQNSGISIHNSQ 360
           FYKQCYVYGTIDFIFGNAAVVLQNCMIYVRRPLKGQVNVITAQGREDP+QNSGISIHNSQ
Sbjct: 360 FYKQCYVYGTIDFIFGNAAVVLQNCMIYVRRPLKGQVNVITAQGREDPFQNSGISIHNSQ 419

Query: 361 IRAAPDLRPVIGSVKTYLGRPWKKYSRTVIMRSYIDSLVSPMGWLAWQSSKFAQDTLYYG 420
           IRAA DLRP++GSVKTYLGRPWKKYSRTVIMRSYID LVSP GWLAWQSSKFAQ TLYYG
Sbjct: 420 IRAAADLRPMVGSVKTYLGRPWKKYSRTVIMRSYIDWLVSPAGWLAWQSSKFAQATLYYG 479

Query: 421 EFKNFGPRASTRYRVRWPGFHVIRSPNVASQFTVRRLIAGQTWLPATGVPFTLGL 463
           E++N GPRASTR+RV+WPGFHVI+SPNVAS+F+V+RLIAGQTWLPATGVPF LG+
Sbjct: 480 EYRNIGPRASTRFRVKWPGFHVIKSPNVASKFSVQRLIAGQTWLPATGVPFKLGV 529

[Close]

BLAST of Moc10g02350 vs. NCBI nr
Match: KAG7015521.1 (putative pectinesterase/pectinesterase inhibitor 33, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 765.4 bits (1975), Expect = 2.8e-217
Identity = 385/471 (81.74%), Postives = 420/471 (89.17%), Query Frame = 0


Query: 1   MAIQTALEKAAEAQHYATRFGQSCKNTRQRAAWADCLKLYHDAVFQLNRTLQ----GLSR 60
           MAI+T LE AA+AQ+Y +RFG +CK TRQRAAW DC  LY+D VFQLNRT+Q    G   
Sbjct: 66  MAIKTTLEGAAKAQNYVSRFGPTCKTTRQRAAWTDCFNLYNDTVFQLNRTIQCLDGGNRL 125

Query: 61  KGCTDFDAQTWLSSALTSIDTCLSGAADLNVTDFIAPIRFQNVSEMISNCLAINGAFLGD 120
           + CTDFDAQTWLSSALT+ID C SGAADLNVTDFI PI+ QNVS+MISNCLAING FL +
Sbjct: 126 RHCTDFDAQTWLSSALTNIDMCSSGAADLNVTDFITPIKCQNVSKMISNCLAINGRFLQE 185

Query: 121 GKN----GTEE-ENGFPMWVSSGDRKLLQSSSTKVRANLVVARDGSGNFRTVQAAINAAA 180
                  GTEE +NGFP WVSSGDRKLLQS+  +VRA+LVVA+DGSG FRTVQAAINAAA
Sbjct: 186 ETKSRTMGTEEYQNGFPTWVSSGDRKLLQSA--RVRAHLVVAKDGSGKFRTVQAAINAAA 245

Query: 181 RRRGRGRFVIYVKRGVYRENIEVENDNGNLMLVGDGMRFTVITSGRSVAAGFTTFSSATA 240
           RRRGR RFVIY+KRGVY+ENIEV NDN N+MLVGDG+RFTVITSGRSVAAGFTTF+SATA
Sbjct: 246 RRRGRSRFVIYIKRGVYKENIEVGNDNANIMLVGDGIRFTVITSGRSVAAGFTTFNSATA 305

Query: 241 GIEGPAFIARDITFVNTAGPRMGQAVALRSSSDLSVFHRCGFQGYQDTLMVLSQRQFYKQ 300
           GI+GP F+AR I FVNTAGPRMGQAVALRSSSDLSVFHRC F+GYQDTLMVLSQRQFYKQ
Sbjct: 306 GIQGPGFMARGIRFVNTAGPRMGQAVALRSSSDLSVFHRCSFEGYQDTLMVLSQRQFYKQ 365

Query: 301 CYVYGTIDFIFGNAAVVLQNCMIYVRRPLKGQVNVITAQGREDPYQNSGISIHNSQIRAA 360
           CYVYGTIDFIFGNAAVVLQNC+IYVRRPLKGQVNVITAQGREDP+QNSGISIHNSQIRAA
Sbjct: 366 CYVYGTIDFIFGNAAVVLQNCLIYVRRPLKGQVNVITAQGREDPFQNSGISIHNSQIRAA 425

Query: 361 PDLRPVIGSVKTYLGRPWKKYSRTVIMRSYIDSLVSPMGWLAWQSSKFAQDTLYYGEFKN 420
            DL+P+IGSVKTYLGRPWKKYSRTVIMRSYIDSLVSPMGWLAWQ ++FAQDTLYYGE++N
Sbjct: 426 ADLQPMIGSVKTYLGRPWKKYSRTVIMRSYIDSLVSPMGWLAWQGTRFAQDTLYYGEYRN 485

Query: 421 FGPRASTRYRVRWPGFHVIRSPNVASQFTVRRLIAGQTWLPATGVPFTLGL 463
           FGPRASTR RVRWPGFH I + NVAS+FTVRRLIAG TWLPATGVPF L L
Sbjct: 486 FGPRASTRLRVRWPGFHAITTRNVASKFTVRRLIAGHTWLPATGVPFKLDL 534

[Close]

BLAST of Moc10g02350 vs. NCBI nr
Match: XP_022985199.1 (probable pectinesterase/pectinesterase inhibitor 33 [Cucurbita maxima])

HSP 1 Score: 765.0 bits (1974), Expect = 3.7e-217
Identity = 384/471 (81.53%), Postives = 420/471 (89.17%), Query Frame = 0


Query: 1   MAIQTALEKAAEAQHYATRFGQSCKNTRQRAAWADCLKLYHDAVFQLNRTLQ----GLSR 60
           MAI+T LE AA+AQ+Y +RFG +CK TRQRAAW DC  LY+D VFQLNRT++    G   
Sbjct: 66  MAIKTTLEGAAKAQNYVSRFGPTCKTTRQRAAWTDCFNLYNDTVFQLNRTIRCLHAGTRL 125

Query: 61  KGCTDFDAQTWLSSALTSIDTCLSGAADLNVTDFIAPIRFQNVSEMISNCLAINGAFL-G 120
           + CTDFDAQTWLSSALT+ID C SGAADLNVTDFI PI+ QNVS MISNCLAING FL G
Sbjct: 126 RHCTDFDAQTWLSSALTNIDMCSSGAADLNVTDFITPIKCQNVSRMISNCLAINGRFLEG 185

Query: 121 DGKNGT----EEENGFPMWVSSGDRKLLQSSSTKVRANLVVARDGSGNFRTVQAAINAAA 180
           + K+ T    E +NGFP WVSSGDRKLLQS+  +VRA+LVVA+DGSG FRTVQAAINAAA
Sbjct: 186 ETKSRTTGAEEYQNGFPRWVSSGDRKLLQSA--RVRAHLVVAKDGSGKFRTVQAAINAAA 245

Query: 181 RRRGRGRFVIYVKRGVYRENIEVENDNGNLMLVGDGMRFTVITSGRSVAAGFTTFSSATA 240
           RRRGR RFVIY+KRGVYRENIE+ NDN N+MLVGDG+RFTVITSGRSVAAGFTTF+SATA
Sbjct: 246 RRRGRSRFVIYIKRGVYRENIEIGNDNANIMLVGDGIRFTVITSGRSVAAGFTTFNSATA 305

Query: 241 GIEGPAFIARDITFVNTAGPRMGQAVALRSSSDLSVFHRCGFQGYQDTLMVLSQRQFYKQ 300
           GI+GP F+AR I FVNTAGPRMGQAVALRSSSDLSVFHRC F+GYQDTLMVLSQRQFYKQ
Sbjct: 306 GIQGPGFMARGIRFVNTAGPRMGQAVALRSSSDLSVFHRCSFEGYQDTLMVLSQRQFYKQ 365

Query: 301 CYVYGTIDFIFGNAAVVLQNCMIYVRRPLKGQVNVITAQGREDPYQNSGISIHNSQIRAA 360
           CYVYGTIDFIFGNAAVVLQNC+IYVRRPLKGQVNVITAQGREDP+QNSGISIHNSQIRAA
Sbjct: 366 CYVYGTIDFIFGNAAVVLQNCLIYVRRPLKGQVNVITAQGREDPFQNSGISIHNSQIRAA 425

Query: 361 PDLRPVIGSVKTYLGRPWKKYSRTVIMRSYIDSLVSPMGWLAWQSSKFAQDTLYYGEFKN 420
            DL+P+IGSVKTYLGRPWKKYSRTVIMRSYIDSLVSPMGWLAWQ ++FAQDTLYYGE++N
Sbjct: 426 ADLQPMIGSVKTYLGRPWKKYSRTVIMRSYIDSLVSPMGWLAWQGTRFAQDTLYYGEYRN 485

Query: 421 FGPRASTRYRVRWPGFHVIRSPNVASQFTVRRLIAGQTWLPATGVPFTLGL 463
           FGPRASTR RVRWPGFH I + NVAS+FTVRRLIAG TWLPATGVPF L L
Sbjct: 486 FGPRASTRLRVRWPGFHAITTRNVASKFTVRRLIAGHTWLPATGVPFKLDL 534

[Close]

BLAST of Moc10g02350 vs. ExPASy Swiss-Prot
Match: Q9FHN5 (Probable pectinesterase/pectinesterase inhibitor 59 OS=Arabidopsis thaliana OX=3702 GN=PME59 PE=2 SV=1)

HSP 1 Score: 583.9 bits (1504), Expect = 1.5e-165
Identity = 288/472 (61.02%), Postives = 366/472 (77.54%), Query Frame = 0


Query: 1   MAIQTALEKAAEAQHYATRFGQSCKNTRQRAAWADCLKLYHDAVFQLNRTLQGLS----- 60
           M ++ A+++A  A+   T  G++C +++++A  ADC+ LY D + QLNRTL G+S     
Sbjct: 69  MLVEAAMDRAISARAELTNSGKNCTDSKKQAVLADCIDLYGDTIMQLNRTLHGVSPKAGA 128

Query: 61  RKGCTDFDAQTWLSSALTSIDTCLSGAADLNVTDFIAPI-RFQNVSEMISNCLAINGAFL 120
            K CTDFDAQTWLS+ALT+ +TC  G++DLNVTDFI PI     +S +ISNCLA+NGA L
Sbjct: 129 AKSCTDFDAQTWLSTALTNTETCRRGSSDLNVTDFITPIVSNTKISHLISNCLAVNGALL 188

Query: 121 GDGKNG--TEEENGFPMWVSSGDRKLLQSSSTKVRANLVVARDGSGNFRTVQAAINAAAR 180
             G  G  T  + GFP W+S  D++LL++    VRANLVVA+DGSG+F TVQAAI+ A R
Sbjct: 189 TAGNKGNTTANQKGFPTWLSRKDKRLLRA----VRANLVVAKDGSGHFNTVQAAIDVAGR 248

Query: 181 RR-GRGRFVIYVKRGVYRENIEVENDNGNLMLVGDGMRFTVITSGRSVAAGFTTFSSATA 240
           R+   GRFVIYVKRG+Y+ENI V  +N ++MLVGDGMR T+IT GRSV  G+TT++SATA
Sbjct: 249 RKVTSGRFVIYVKRGIYQENINVRLNNDDIMLVGDGMRSTIITGGRSVQGGYTTYNSATA 308

Query: 241 GIEGPAFIARDITFVNTAGPRMGQAVALRSSSDLSVFHRCGFQGYQDTLMVLSQRQFYKQ 300
           GIEG  FIA+ ITF NTAGP  GQAVALRSSSDLS+F++C  +GYQDTLMV SQRQFY++
Sbjct: 309 GIEGLHFIAKGITFRNTAGPAKGQAVALRSSSDLSIFYKCSIEGYQDTLMVHSQRQFYRE 368

Query: 301 CYVYGTIDFIFGNAAVVLQNCMIYVRRPLKGQVNVITAQGREDPYQNSGISIHNSQIRAA 360
           CY+YGT+DFIFGNAA V QNC+I  RRPLKGQ NVITAQGR DP+QN+GISIHNS+I  A
Sbjct: 369 CYIYGTVDFIFGNAAAVFQNCLILPRRPLKGQANVITAQGRADPFQNTGISIHNSRILPA 428

Query: 361 PDLRPVIGSVKTYLGRPWKKYSRTVIMRSYIDSLVSPMGWLAW-QSSKFAQDTLYYGEFK 420
           PDL+PV+G+VKTY+GRPW K+SRTV++++Y+D++VSP+GW  W + S F  DTL+Y E+K
Sbjct: 429 PDLKPVVGTVKTYMGRPWMKFSRTVVLQTYLDNVVSPVGWSPWIEGSVFGLDTLFYAEYK 488

Query: 421 NFGPRASTRYRVRWPGFHVIRSPNVASQFTVRRLIAGQTWLPATGVPFTLGL 463
           N GP +STR+RV W GFHV+   + AS FTV + IAG  WLP TG+PFT GL
Sbjct: 489 NTGPASSTRWRVSWKGFHVLGRASDASAFTVGKFIAGTAWLPRTGIPFTSGL 536

[Close]

BLAST of Moc10g02350 vs. ExPASy Swiss-Prot
Match: Q9FHN4 (Probable pectinesterase/pectinesterase inhibitor 60 OS=Arabidopsis thaliana OX=3702 GN=PME60 PE=2 SV=1)

HSP 1 Score: 576.2 bits (1484), Expect = 3.2e-163
Identity = 288/467 (61.67%), Postives = 360/467 (77.09%), Query Frame = 0


Query: 1   MAIQTALEKAAEAQHYATRFGQSCKNTRQRAAWADCLKLYHDAVFQLNRTLQGLSRK--- 60
           + ++ A+++A  A    T   ++C + +++A  ADC+ LY D V QLNRTLQG+S K   
Sbjct: 73  LLVEAAMDRAVSAWDKLTNSSKNCTDFKKQAVLADCINLYGDTVMQLNRTLQGVSSKTGR 132

Query: 61  GCTDFDAQTWLSSALTSIDTCLSGAADLNVTDFIAPI-RFQNVSEMISNCLAINGAFLGD 120
            CTDFDAQTWLS+ALT+ +TC  G++DLNV+DF  PI     +S +ISNCLA+NGA L  
Sbjct: 133 RCTDFDAQTWLSTALTNTETCRRGSSDLNVSDFTTPIVSNTKISHLISNCLAVNGALLTA 192

Query: 121 GKNG--TEEENGFPMWVSSGDRKLLQSSSTKVRANLVVARDGSGNFRTVQAAINAAARRR 180
           GKN   T +  GFP WVS  +R+LLQ  S  VRANLVVA+DGSG+F+TVQAAI+ A RR+
Sbjct: 193 GKNDSTTGDSKGFPTWVSRKERRLLQLQS--VRANLVVAKDGSGHFKTVQAAIDVAGRRK 252

Query: 181 -GRGRFVIYVKRGVYRENIEVENDNGNLMLVGDGMRFTVITSGRSVAAGFTTFSSATAGI 240
              GRFVIYVKRG+Y+EN+ V  +N N+MLVGDGMR+T+IT GRSV  G+TT+SSATAGI
Sbjct: 253 VTSGRFVIYVKRGIYQENLNVRLNNDNIMLVGDGMRYTIITGGRSVKGGYTTYSSATAGI 312

Query: 241 EGPAFIARDITFVNTAGPRMGQAVALRSSSDLSVFHRCGFQGYQDTLMVLSQRQFYKQCY 300
           EG  FIA+ I F NTAGP  GQAVALRSSSDLS+F+RC  +GYQDTLMV SQRQFY++CY
Sbjct: 313 EGLHFIAKGIAFQNTAGPAKGQAVALRSSSDLSIFYRCSIEGYQDTLMVHSQRQFYRECY 372

Query: 301 VYGTIDFIFGNAAVVLQNCMIYVRRPLKGQVNVITAQGREDPYQNSGISIHNSQIRAAPD 360
           +YGT+DFIFGNAAVV QNC+I  R PLKGQ NVITAQGR D +QN+GISIHNS I  APD
Sbjct: 373 IYGTVDFIFGNAAVVFQNCIILPRLPLKGQANVITAQGRTDLFQNTGISIHNSIIIPAPD 432

Query: 361 LRPVIGSVKTYLGRPWKKYSRTVIMRSYIDSLVSPMGWLAW-QSSKFAQDTLYYGEFKNF 420
           L+PV+ SVKTY+GRPW  YSRTV++++YIDS+VSP+GW  W + S +  DTL+Y E+KN 
Sbjct: 433 LKPVVRSVKTYMGRPWMMYSRTVVLKTYIDSVVSPVGWSPWTKGSTYGLDTLFYAEYKNI 492

Query: 421 GPRASTRYRVRWPGFHVIRSPNVASQFTVRRLIAGQTWLPATGVPFT 460
           GP +STR+RVRW GFHV+   + AS F+V + IAG  WLP +G+PFT
Sbjct: 493 GPASSTRWRVRWKGFHVLSKASDASAFSVGKFIAGTAWLPGSGIPFT 537

[Close]

BLAST of Moc10g02350 vs. ExPASy Swiss-Prot
Match: Q9STY3 (Probable pectinesterase/pectinesterase inhibitor 33 OS=Arabidopsis thaliana OX=3702 GN=PME33 PE=2 SV=1)

HSP 1 Score: 570.5 bits (1469), Expect = 1.8e-161
Identity = 286/472 (60.59%), Postives = 363/472 (76.91%), Query Frame = 0


Query: 1   MAIQTALEKAAEAQHYATRFGQSCKNTRQRAAWADCLKLYHDAVFQLNRTLQGL-----S 60
           M ++ AL++A        +FG SC N +++AAW+DC+ L+ + V QLNRTL+GL     S
Sbjct: 123 MLVKVALDQAVITHSQTVKFGPSCTNNQRKAAWSDCVNLFQNTVAQLNRTLKGLNPAASS 182

Query: 61  RKGCTDFDAQTWLSSALTSIDTCLSGAADLNVTDFIAP-IRFQNVSEMISNCLAINGAFL 120
              CTDFDAQTWLS+A T+I+TC SG+ DLNV+DF+ P I  +N+S++I NCLA+NG  +
Sbjct: 183 DVKCTDFDAQTWLSTAQTNIETCRSGSEDLNVSDFVMPVISNKNLSDLIGNCLAVNGVLM 242

Query: 121 --GDGKNGTEEENGFPMWVSSGDRKLLQSSS-TKVRANLVVARDGSGNFRTVQAAINAAA 180
              D       +  FP WVS  +R+LL S+S  K   +LVVA+D SG+FR++QAAIN AA
Sbjct: 243 KQHDHTTTANHKEYFPSWVSRHERRLLVSASLAKSSPHLVVAQDRSGHFRSIQAAINFAA 302

Query: 181 RRRGRGRFVIYVKRGVYRENIEVENDNGNLMLVGDGMRFTVITSGRSVAAGFTTFSSATA 240
           RRR + RFVIYVK+GVYRENI+V NDN N+MLVGDG R T+ITSGRSV  G+TT++SAT 
Sbjct: 303 RRRFKSRFVIYVKKGVYRENIDVGNDNHNIMLVGDGERKTIITSGRSVQHGYTTYNSATG 362

Query: 241 GIEGPAFIARDITFVNTAGPRMGQAVALRSSSDLSVFHRCGFQGYQDTLMVLSQRQFYKQ 300
           G  G  F+A+D+TF+NTAGP  GQAVA+RSSSDLSVF+R G  G+QDTL + SQRQF+++
Sbjct: 363 GFGGQRFVAKDMTFINTAGPLRGQAVAVRSSSDLSVFYRVGIHGFQDTLYIHSQRQFFRE 422

Query: 301 CYVYGTIDFIFGNAAVVLQNCMIYVRRPLKGQVNVITAQGREDPYQNSGISIHNSQIRAA 360
           CY+ GTIDFIFGNAAVV QNCMI VRRPL GQ N+ITAQGR DP+QN+GI+IH+S+I AA
Sbjct: 423 CYISGTIDFIFGNAAVVFQNCMILVRRPLHGQANIITAQGRGDPFQNTGITIHSSRIIAA 482

Query: 361 PDLRPVIGSVKTYLGRPWKKYSRTVIMRSYIDSLVSPMGWLAW-QSSKFAQDTLYYGEFK 420
            DL+PVI + KTYLGRPW+ YSR  IM++YID+ +SP+GW  W + S FA +T++YGE+K
Sbjct: 483 SDLKPVIRAYKTYLGRPWQAYSRVTIMKTYIDNSISPLGWSPWLRGSNFALNTVFYGEYK 542

Query: 421 NFGPRASTRYRVRWPGFHVIRSPNVASQFTVRRLIAGQTWLPATGVPFTLGL 463
           NFGP +STR+RVRW GFH I S  VAS+FTV  LIAG +WLPATGVPF  GL
Sbjct: 543 NFGPGSSTRWRVRWKGFHAITSTAVASRFTVGSLIAGGSWLPATGVPFKSGL 594

[Close]

BLAST of Moc10g02350 vs. ExPASy Swiss-Prot
Match: O04887 (Pectinesterase 2 OS=Citrus sinensis OX=2711 GN=PECS-2.1 PE=2 SV=1)

HSP 1 Score: 500.4 bits (1287), Expect = 2.2e-140
Identity = 256/462 (55.41%), Postives = 328/462 (71.00%), Query Frame = 0


Query: 1   MAIQTALEKAAEAQHYATRFGQSCKNTRQRAAWADCLKLYHDAVFQLNRTLQGLSRKGCT 60
           +++Q ALE+A  AQ      G  C+N R++AAW DC +LY   V +LN+T    S  GCT
Sbjct: 63  ISLQLALERATTAQSRTYTLGSKCRNEREKAAWEDCRELYELTVLKLNQTSN--SSPGCT 122

Query: 61  DFDAQTWLSSALTSIDTCLSGAADLNVTDFIAPIRFQNVSEMISNCLAINGAFLGDGKNG 120
             D QTWLS+ALT+++TC +   DL V +++ P+   NV+++ISN L++N        N 
Sbjct: 123 KVDKQTWLSTALTNLETCRASLEDLGVPEYVLPLLSNNVTKLISNTLSLNKV----PYNE 182

Query: 121 TEEENGFPMWVSSGDRKLLQSSSTKVRANLVVARDGSGNFRTVQAAINAAARRRGRGRFV 180
              ++GFP WV  GDRKLLQ   T  RAN+VVA+DGSGN +T+Q A+ AAA R G  R+V
Sbjct: 183 PSYKDGFPTWVKPGDRKLLQ---TTPRANIVVAQDGSGNVKTIQEAV-AAASRAGGSRYV 242

Query: 181 IYVKRGVYRENIEVENDNGNLMLVGDGMRFTVITSGRSVAAGFTTFSSATAGIEGPAFIA 240
           IY+K G Y ENIEV+    N+M VGDG+  T+IT  +SV  G TTF SAT  + G  FIA
Sbjct: 243 IYIKAGTYNENIEVKLK--NIMFVGDGIGKTIITGSKSVGGGATTFKSATVAVVGDNFIA 302

Query: 241 RDITFVNTAGPRMGQAVALRSSSDLSVFHRCGFQGYQDTLMVLSQRQFYKQCYVYGTIDF 300
           RDIT  NTAGP   QAVALRS SDLSVF+RC F+GYQDTL V SQRQFY++C +YGT+DF
Sbjct: 303 RDITIRNTAGPNNHQAVALRSGSDLSVFYRCSFEGYQDTLYVHSQRQFYRECDIYGTVDF 362

Query: 301 IFGNAAVVLQNCMIYVRRPLKGQVNVITAQGREDPYQNSGISIHNSQIRAAPDLRPVIGS 360
           IFGNAAVVLQNC I+ R+P   + N +TAQGR DP Q++GI IHN ++ AA DL+PV  S
Sbjct: 363 IFGNAAVVLQNCNIFARKP-PNRTNTLTAQGRTDPNQSTGIIIHNCRVTAASDLKPVQSS 422

Query: 361 VKTYLGRPWKKYSRTVIMRSYIDSLVSPMGWLAWQSSKFAQDTLYYGEFKNFGPRASTRY 420
           VKT+LGRPWK+YSRTV +++++DSL++P GW+ W S  FA +TLYY E+ N GP +ST  
Sbjct: 423 VKTFLGRPWKQYSRTVYIKTFLDSLINPAGWMEW-SGDFALNTLYYAEYMNTGPGSSTAN 482

Query: 421 RVRWPGFHVIRSPNVASQFTVRRLIAGQTWLPATGVPFTLGL 463
           RV+W G+HV+ SP+  SQFTV   IAG +WLPAT VPFT GL
Sbjct: 483 RVKWRGYHVLTSPSQVSQFTVGNFIAGNSWLPATNVPFTSGL 510

[Close]

BLAST of Moc10g02350 vs. ExPASy Swiss-Prot
Match: O22149 (Probable pectinesterase/pectinesterase inhibitor 17 OS=Arabidopsis thaliana OX=3702 GN=PME17 PE=2 SV=2)

HSP 1 Score: 487.6 bits (1254), Expect = 1.5e-136
Identity = 255/462 (55.19%), Postives = 327/462 (70.78%), Query Frame = 0


Query: 1   MAIQTALEKAAEAQHYATRFGQSCKNTRQRAAWADCLKLYHDAVFQLNRTLQGLSRKGCT 60
           ++++  L++A  A+ +A   G  C++TR++AAW DC+KLY   V ++N T+       C+
Sbjct: 65  ISMKLVLDRAILAKTHAFTLGPKCRDTREKAAWEDCIKLYDLTVSKINETMD--PNVKCS 124

Query: 61  DFDAQTWLSSALTSIDTCLSGAADLNVTDFIAPIRFQNVSEMISNCLAINGAFLGDGKNG 120
             DAQTWLS+ALT++DTC +G  +L VTD + P+   NVS ++ N LAIN          
Sbjct: 125 KLDAQTWLSTALTNLDTCRAGFLELGVTDIVLPLMSNNVSNLLCNTLAINKV---PFNYT 184

Query: 121 TEEENGFPMWVSSGDRKLLQSSSTKVRANLVVARDGSGNFRTVQAAINAAARRRGRGRFV 180
             E++GFP WV  GDRKLLQSS+ K   N VVA+DGSGNF+T++ AI+AA+   G GRFV
Sbjct: 185 PPEKDGFPSWVKPGDRKLLQSSTPK--DNAVVAKDGSGNFKTIKEAIDAAS---GSGRFV 244

Query: 181 IYVKRGVYRENIEVENDNGNLMLVGDGMRFTVITSGRSVAAGFTTFSSATAGIEGPAFIA 240
           IYVK+GVY EN+E+     N+ML GDG+  T+IT  +SV  G TTF+SAT    G  FIA
Sbjct: 245 IYVKQGVYSENLEIRKK--NVMLRGDGIGKTIITGSKSVGGGTTTFNSATVAAVGDGFIA 304

Query: 241 RDITFVNTAGPRMGQAVALRSSSDLSVFHRCGFQGYQDTLMVLSQRQFYKQCYVYGTIDF 300
           R ITF NTAG    QAVALRS SDLSVF++C F+ YQDTL V S RQFY+ C VYGT+DF
Sbjct: 305 RGITFRNTAGASNEQAVALRSGSDLSVFYQCSFEAYQDTLYVHSNRQFYRDCDVYGTVDF 364

Query: 301 IFGNAAVVLQNCMIYVRRPLKGQVNVITAQGREDPYQNSGISIHNSQIRAAPDLRPVIGS 360
           IFGNAA VLQNC I+ RRP + + N ITAQGR DP QN+GI IHNS++ AA DLRPV+GS
Sbjct: 365 IFGNAAAVLQNCNIFARRP-RSKTNTITAQGRSDPNQNTGIIIHNSRVTAASDLRPVLGS 424

Query: 361 VKTYLGRPWKKYSRTVIMRSYIDSLVSPMGWLAWQSSKFAQDTLYYGEFKNFGPRASTRY 420
            KTYLGRPW++YSRTV M++ +DSL+ P GWL W  + FA  TL+Y EF+N GP AST  
Sbjct: 425 TKTYLGRPWRQYSRTVFMKTSLDSLIDPRGWLEWDGN-FALKTLFYAEFQNTGPGASTSG 484

Query: 421 RVRWPGFHVIRSPNVASQFTVRRLIAGQTWLPATGVPFTLGL 463
           RV WPGF V+ S + AS+FTV   +AG +W+P++ VPFT GL
Sbjct: 485 RVTWPGFRVLGSASEASKFTVGTFLAGGSWIPSS-VPFTSGL 511

[Close]

BLAST of Moc10g02350 vs. ExPASy TrEMBL
Match: A0A1S3BUR4 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103493398 PE=3 SV=1)

HSP 1 Score: 776.2 bits (2003), Expect = 7.8e-221
Identity = 392/476 (82.35%), Postives = 418/476 (87.82%), Query Frame = 0


Query: 1   MAIQTALEKAAEAQHYATRFGQSCKNTRQRAAWADCLKLYHDAVFQLNRTLQGL------ 60
           +AIQT LEKA EA+ Y  RFG +CK TRQR AW DC KLY+D VFQLNRTLQ L      
Sbjct: 63  VAIQTTLEKADEAKAYTARFGPTCKTTRQRTAWTDCFKLYNDVVFQLNRTLQCLVTDETI 122

Query: 61  -SRKGCTDFDAQTWLSSALTSIDTCLSGAADLNVTDFIAPIRFQNVSEMISNCLAINGAF 120
             R  CTDFDAQTWLSSALT ID C SGAADLNVTDFIAPI+  NVS+MISNCLAING F
Sbjct: 123 HRRPTCTDFDAQTWLSSALTDIDLCNSGAADLNVTDFIAPIKCLNVSKMISNCLAINGGF 182

Query: 121 L-------GDGKNGTEEENGFPMWVSSGDRKLLQSSSTKVRANLVVARDGSGNFRTVQAA 180
           L        DG NG+     FPMWVS GDRKLL+S   +VRANLVVA+DGSG FR VQAA
Sbjct: 183 LEEEGVKYDDGGNGS-----FPMWVSEGDRKLLESRPRRVRANLVVAKDGSGKFRRVQAA 242

Query: 181 INAAARRRGRGRFVIYVKRGVYRENIEVENDNGNLMLVGDGMRFTVITSGRSVAAGFTTF 240
           I+AAARRRGRGRFVIYVKRGVYRENIEV NDNGN+MLVGDGMRFTVITSGRSVAAGFTTF
Sbjct: 243 IDAAARRRGRGRFVIYVKRGVYRENIEVGNDNGNIMLVGDGMRFTVITSGRSVAAGFTTF 302

Query: 241 SSATAGIEGPAFIARDITFVNTAGPRMGQAVALRSSSDLSVFHRCGFQGYQDTLMVLSQR 300
           SSATAGI+GP FIARDI FVNTAGPRMGQAVALRSSSDLSVFHRC F+GYQDTLMVLSQR
Sbjct: 303 SSATAGIQGPGFIARDIRFVNTAGPRMGQAVALRSSSDLSVFHRCSFEGYQDTLMVLSQR 362

Query: 301 QFYKQCYVYGTIDFIFGNAAVVLQNCMIYVRRPLKGQVNVITAQGREDPYQNSGISIHNS 360
           QFYKQCY+YGTIDFIFGNAAVVLQNC+IYVRRPLKGQVNVITAQGREDP+QNSGISIHNS
Sbjct: 363 QFYKQCYIYGTIDFIFGNAAVVLQNCLIYVRRPLKGQVNVITAQGREDPFQNSGISIHNS 422

Query: 361 QIRAAPDLRPVIGSVKTYLGRPWKKYSRTVIMRSYIDSLVSPMGWLAWQSSKFAQDTLYY 420
           QIRAA DLRP++GSVKTYLGRPWKKYSRTVIMRSYIDSLVSP GWLAWQSSKFAQ TLYY
Sbjct: 423 QIRAAADLRPMVGSVKTYLGRPWKKYSRTVIMRSYIDSLVSPAGWLAWQSSKFAQATLYY 482

Query: 421 GEFKNFGPRASTRYRVRWPGFHVIRSPNVASQFTVRRLIAGQTWLPATGVPFTLGL 463
           GE+ N GPRASTR+RV+WPGFHVI+SPNVAS+F+VRRLIAGQTWLPATGVPF LG+
Sbjct: 483 GEYHNIGPRASTRFRVKWPGFHVIKSPNVASKFSVRRLIAGQTWLPATGVPFKLGV 533

[Close]

BLAST of Moc10g02350 vs. ExPASy TrEMBL
Match: A0A0A0L330 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_3G088980 PE=3 SV=1)

HSP 1 Score: 775.4 bits (2001), Expect = 1.3e-220
Identity = 388/475 (81.68%), Postives = 419/475 (88.21%), Query Frame = 0


Query: 1   MAIQTALEKAAEAQHYATRFGQSCKNTRQRAAWADCLKLYHDAVFQLNRTL------QGL 60
           MAIQT LEKAAEA+ Y  RFG +CK +RQR AW DC KLY+D V QLNRTL      + +
Sbjct: 60  MAIQTTLEKAAEARAYTARFGPTCKTSRQRTAWTDCFKLYNDVVLQLNRTLHCVVTDEAI 119

Query: 61  SRKGCTDFDAQTWLSSALTSIDTCLSGAADLNVTDFIAPIRFQNVSEMISNCLAINGAFL 120
            R+ CTDFDAQTWLSSALT ID C SGAADLNVTDFI PI+  NVS+MISNCLAING FL
Sbjct: 120 HRRSCTDFDAQTWLSSALTDIDLCNSGAADLNVTDFITPIKCLNVSKMISNCLAINGGFL 179

Query: 121 -------GDGKNGTEEENGFPMWVSSGDRKLLQSSSTKVRANLVVARDGSGNFRTVQAAI 180
                   DG+NG+     FPMWVS GDRKLL+S   +VRANLVVA+DGSG FR VQAAI
Sbjct: 180 EEEGVKYDDGRNGS-----FPMWVSEGDRKLLESRPGRVRANLVVAKDGSGTFRRVQAAI 239

Query: 181 NAAARRRGRGRFVIYVKRGVYRENIEVENDNGNLMLVGDGMRFTVITSGRSVAAGFTTFS 240
           +AAARRRGRGRF+IYVKRGVYRENIEV NDNGN+MLVGDGMRFTVITSGRSVAAGFTTFS
Sbjct: 240 DAAARRRGRGRFIIYVKRGVYRENIEVGNDNGNIMLVGDGMRFTVITSGRSVAAGFTTFS 299

Query: 241 SATAGIEGPAFIARDITFVNTAGPRMGQAVALRSSSDLSVFHRCGFQGYQDTLMVLSQRQ 300
           SATAGI+GP FIARDI FVNTAGPRMGQAVALRSSSDLSVFHRC F+GYQDTLMVLSQRQ
Sbjct: 300 SATAGIQGPGFIARDIRFVNTAGPRMGQAVALRSSSDLSVFHRCSFEGYQDTLMVLSQRQ 359

Query: 301 FYKQCYVYGTIDFIFGNAAVVLQNCMIYVRRPLKGQVNVITAQGREDPYQNSGISIHNSQ 360
           FYKQCYVYGTIDFIFGNAAVVLQNCMIYVRRPLKGQVNVITAQGREDP+QNSGISIHNSQ
Sbjct: 360 FYKQCYVYGTIDFIFGNAAVVLQNCMIYVRRPLKGQVNVITAQGREDPFQNSGISIHNSQ 419

Query: 361 IRAAPDLRPVIGSVKTYLGRPWKKYSRTVIMRSYIDSLVSPMGWLAWQSSKFAQDTLYYG 420
           IRAA DLRP++GSVKTYLGRPWKKYSRTVIMRSYID LVSP GWLAWQSSKFAQ TLYYG
Sbjct: 420 IRAAADLRPMVGSVKTYLGRPWKKYSRTVIMRSYIDWLVSPAGWLAWQSSKFAQATLYYG 479

Query: 421 EFKNFGPRASTRYRVRWPGFHVIRSPNVASQFTVRRLIAGQTWLPATGVPFTLGL 463
           E++N GPRASTR+RV+WPGFHVI+SPNVAS+F+V+RLIAGQTWLPATGVPF LG+
Sbjct: 480 EYRNIGPRASTRFRVKWPGFHVIKSPNVASKFSVQRLIAGQTWLPATGVPFKLGV 529

[Close]

BLAST of Moc10g02350 vs. ExPASy TrEMBL
Match: A0A6J1JCM7 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111483275 PE=3 SV=1)

HSP 1 Score: 765.0 bits (1974), Expect = 1.8e-217
Identity = 384/471 (81.53%), Postives = 420/471 (89.17%), Query Frame = 0


Query: 1   MAIQTALEKAAEAQHYATRFGQSCKNTRQRAAWADCLKLYHDAVFQLNRTLQ----GLSR 60
           MAI+T LE AA+AQ+Y +RFG +CK TRQRAAW DC  LY+D VFQLNRT++    G   
Sbjct: 66  MAIKTTLEGAAKAQNYVSRFGPTCKTTRQRAAWTDCFNLYNDTVFQLNRTIRCLHAGTRL 125

Query: 61  KGCTDFDAQTWLSSALTSIDTCLSGAADLNVTDFIAPIRFQNVSEMISNCLAINGAFL-G 120
           + CTDFDAQTWLSSALT+ID C SGAADLNVTDFI PI+ QNVS MISNCLAING FL G
Sbjct: 126 RHCTDFDAQTWLSSALTNIDMCSSGAADLNVTDFITPIKCQNVSRMISNCLAINGRFLEG 185

Query: 121 DGKNGT----EEENGFPMWVSSGDRKLLQSSSTKVRANLVVARDGSGNFRTVQAAINAAA 180
           + K+ T    E +NGFP WVSSGDRKLLQS+  +VRA+LVVA+DGSG FRTVQAAINAAA
Sbjct: 186 ETKSRTTGAEEYQNGFPRWVSSGDRKLLQSA--RVRAHLVVAKDGSGKFRTVQAAINAAA 245

Query: 181 RRRGRGRFVIYVKRGVYRENIEVENDNGNLMLVGDGMRFTVITSGRSVAAGFTTFSSATA 240
           RRRGR RFVIY+KRGVYRENIE+ NDN N+MLVGDG+RFTVITSGRSVAAGFTTF+SATA
Sbjct: 246 RRRGRSRFVIYIKRGVYRENIEIGNDNANIMLVGDGIRFTVITSGRSVAAGFTTFNSATA 305

Query: 241 GIEGPAFIARDITFVNTAGPRMGQAVALRSSSDLSVFHRCGFQGYQDTLMVLSQRQFYKQ 300
           GI+GP F+AR I FVNTAGPRMGQAVALRSSSDLSVFHRC F+GYQDTLMVLSQRQFYKQ
Sbjct: 306 GIQGPGFMARGIRFVNTAGPRMGQAVALRSSSDLSVFHRCSFEGYQDTLMVLSQRQFYKQ 365

Query: 301 CYVYGTIDFIFGNAAVVLQNCMIYVRRPLKGQVNVITAQGREDPYQNSGISIHNSQIRAA 360
           CYVYGTIDFIFGNAAVVLQNC+IYVRRPLKGQVNVITAQGREDP+QNSGISIHNSQIRAA
Sbjct: 366 CYVYGTIDFIFGNAAVVLQNCLIYVRRPLKGQVNVITAQGREDPFQNSGISIHNSQIRAA 425

Query: 361 PDLRPVIGSVKTYLGRPWKKYSRTVIMRSYIDSLVSPMGWLAWQSSKFAQDTLYYGEFKN 420
            DL+P+IGSVKTYLGRPWKKYSRTVIMRSYIDSLVSPMGWLAWQ ++FAQDTLYYGE++N
Sbjct: 426 ADLQPMIGSVKTYLGRPWKKYSRTVIMRSYIDSLVSPMGWLAWQGTRFAQDTLYYGEYRN 485

Query: 421 FGPRASTRYRVRWPGFHVIRSPNVASQFTVRRLIAGQTWLPATGVPFTLGL 463
           FGPRASTR RVRWPGFH I + NVAS+FTVRRLIAG TWLPATGVPF L L
Sbjct: 486 FGPRASTRLRVRWPGFHAITTRNVASKFTVRRLIAGHTWLPATGVPFKLDL 534

[Close]

BLAST of Moc10g02350 vs. ExPASy TrEMBL
Match: A0A6J1EW59 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111438452 PE=3 SV=1)

HSP 1 Score: 763.5 bits (1970), Expect = 5.2e-217
Identity = 384/471 (81.53%), Postives = 419/471 (88.96%), Query Frame = 0


Query: 1   MAIQTALEKAAEAQHYATRFGQSCKNTRQRAAWADCLKLYHDAVFQLNRTLQ----GLSR 60
           MAI+T LE AA+AQ+Y + FG +CK TRQRAAW DC  LY+D VFQLNRT+Q    G   
Sbjct: 66  MAIKTTLEGAAKAQNYVSLFGPTCKTTRQRAAWTDCFNLYNDTVFQLNRTIQCLDGGTRL 125

Query: 61  KGCTDFDAQTWLSSALTSIDTCLSGAADLNVTDFIAPIRFQNVSEMISNCLAINGAFLGD 120
           + CTDFDAQTWLSSALT+ID C SGAADLNVTDFI PI+ QNVS+MISNCLAING FL +
Sbjct: 126 RHCTDFDAQTWLSSALTNIDMCSSGAADLNVTDFITPIKCQNVSKMISNCLAINGRFLQE 185

Query: 121 ----GKNGTEE-ENGFPMWVSSGDRKLLQSSSTKVRANLVVARDGSGNFRTVQAAINAAA 180
                  GTEE +NGFP WVSSGDRKLLQS+  +VRA+LVVA+DGSG FRTVQAAINAAA
Sbjct: 186 ETKSRTTGTEEYQNGFPTWVSSGDRKLLQSA--RVRAHLVVAKDGSGKFRTVQAAINAAA 245

Query: 181 RRRGRGRFVIYVKRGVYRENIEVENDNGNLMLVGDGMRFTVITSGRSVAAGFTTFSSATA 240
           RRRGR RFVIY+KRGVY+ENIEV NDN N+MLVGDG+RFTVITSGRSVAAGFTTF+SATA
Sbjct: 246 RRRGRSRFVIYIKRGVYKENIEVGNDNANIMLVGDGIRFTVITSGRSVAAGFTTFNSATA 305

Query: 241 GIEGPAFIARDITFVNTAGPRMGQAVALRSSSDLSVFHRCGFQGYQDTLMVLSQRQFYKQ 300
           GI+GP F+AR I FVNTAGPRMGQAVALRSSSDLSVFHRC F+GYQDTLMVLSQRQFYKQ
Sbjct: 306 GIQGPGFMARGIRFVNTAGPRMGQAVALRSSSDLSVFHRCSFEGYQDTLMVLSQRQFYKQ 365

Query: 301 CYVYGTIDFIFGNAAVVLQNCMIYVRRPLKGQVNVITAQGREDPYQNSGISIHNSQIRAA 360
           CYVYGTIDFIFGNAAVVLQNC+IYVRRPLKGQVNVITAQGREDP+QNSGISIHNSQIRAA
Sbjct: 366 CYVYGTIDFIFGNAAVVLQNCLIYVRRPLKGQVNVITAQGREDPFQNSGISIHNSQIRAA 425

Query: 361 PDLRPVIGSVKTYLGRPWKKYSRTVIMRSYIDSLVSPMGWLAWQSSKFAQDTLYYGEFKN 420
            DL+P+IGSVKTYLGRPWKKYSRTVIMRSYIDSLVSPMGWLAWQ ++FAQDTLYYGE++N
Sbjct: 426 ADLQPMIGSVKTYLGRPWKKYSRTVIMRSYIDSLVSPMGWLAWQGTRFAQDTLYYGEYRN 485

Query: 421 FGPRASTRYRVRWPGFHVIRSPNVASQFTVRRLIAGQTWLPATGVPFTLGL 463
           FGPRASTR RVRWPGFH I + NVAS+FTVRRLIAG TWLPATGVPF L L
Sbjct: 486 FGPRASTRLRVRWPGFHAITTRNVASKFTVRRLIAGHTWLPATGVPFKLDL 534

[Close]

BLAST of Moc10g02350 vs. ExPASy TrEMBL
Match: A0A5A7TVK4 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold68G001080 PE=3 SV=1)

HSP 1 Score: 762.3 bits (1967), Expect = 1.2e-216
Identity = 386/476 (81.09%), Postives = 414/476 (86.97%), Query Frame = 0


Query: 1   MAIQTALEKAAEAQHYATRFGQSCKNTRQRAAWADCLKLYHDAVFQLNRTLQGL------ 60
           +AIQT LEKA EA+ Y  RFG +CK TRQR AW DC KLY+D VFQLNRTLQ L      
Sbjct: 17  VAIQTTLEKADEAKAYTARFGPTCKTTRQRTAWTDCFKLYNDVVFQLNRTLQCLVTDETI 76

Query: 61  -SRKGCTDFDAQTWLSSALTSIDTCLSGAADLNVTDFIAPIRFQNVSEMISNCLAINGAF 120
             R  CTDFDAQTWLSSALT ID C SGAADLNVTDFIAPI+  NVS+MISNCLAING F
Sbjct: 77  HRRPTCTDFDAQTWLSSALTDIDLCNSGAADLNVTDFIAPIKCLNVSKMISNCLAINGGF 136

Query: 121 L-------GDGKNGTEEENGFPMWVSSGDRKLLQSSSTKVRANLVVARDGSGNFRTVQAA 180
           +        DG NG+     FPMWVS GDRKLL+S   +VRANLVVA+DGSG FR VQAA
Sbjct: 137 IEEEGVKYDDGGNGS-----FPMWVSEGDRKLLESRPRRVRANLVVAKDGSGKFRRVQAA 196

Query: 181 INAAARRRGRGRFVIYVKRGVYRENIEVENDNGNLMLVGDGMRFTVITSGRSVAAGFTTF 240
           I+AAARRRGRGRFVIYVKRGVYRENIEV NDNGN+MLVGDGMRFTVITSGRSVAAGFTTF
Sbjct: 197 IDAAARRRGRGRFVIYVKRGVYRENIEVGNDNGNIMLVGDGMRFTVITSGRSVAAGFTTF 256

Query: 241 SSATAGIEGPAFIARDITFVNTAGPRMGQAVALRSSSDLSVFHRCGFQGYQDTLMVLSQR 300
           S     I+GP FIARDI FVNTAGPRMGQAVALRSSSDLSVFHRC F+GYQDTLMVLSQR
Sbjct: 257 S-----IQGPGFIARDIRFVNTAGPRMGQAVALRSSSDLSVFHRCSFEGYQDTLMVLSQR 316

Query: 301 QFYKQCYVYGTIDFIFGNAAVVLQNCMIYVRRPLKGQVNVITAQGREDPYQNSGISIHNS 360
           QFYKQCY+YGTIDFIFGNAAVVLQNC+IYVRRPLKGQVNVITAQGREDP+QNSGISIHNS
Sbjct: 317 QFYKQCYIYGTIDFIFGNAAVVLQNCLIYVRRPLKGQVNVITAQGREDPFQNSGISIHNS 376

Query: 361 QIRAAPDLRPVIGSVKTYLGRPWKKYSRTVIMRSYIDSLVSPMGWLAWQSSKFAQDTLYY 420
           QIRAA DLRP++GSVKTYLGRPWKKYSRTVIMRSYIDSLVSP GWLAWQSSKFAQ TLYY
Sbjct: 377 QIRAAADLRPMVGSVKTYLGRPWKKYSRTVIMRSYIDSLVSPAGWLAWQSSKFAQATLYY 436

Query: 421 GEFKNFGPRASTRYRVRWPGFHVIRSPNVASQFTVRRLIAGQTWLPATGVPFTLGL 463
           GE++N GPRASTR+RV+WPGFHVI+SPNVAS+F+VRRLIAGQTWLPATGVPF LG+
Sbjct: 437 GEYRNIGPRASTRFRVKWPGFHVIKSPNVASKFSVRRLIAGQTWLPATGVPFKLGV 482

[Close]

BLAST of Moc10g02350 vs. TAIR 10
Match: AT5G51490.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 583.9 bits (1504), Expect = 1.1e-166
Identity = 288/472 (61.02%), Postives = 366/472 (77.54%), Query Frame = 0


Query: 1   MAIQTALEKAAEAQHYATRFGQSCKNTRQRAAWADCLKLYHDAVFQLNRTLQGLS----- 60
           M ++ A+++A  A+   T  G++C +++++A  ADC+ LY D + QLNRTL G+S     
Sbjct: 69  MLVEAAMDRAISARAELTNSGKNCTDSKKQAVLADCIDLYGDTIMQLNRTLHGVSPKAGA 128

Query: 61  RKGCTDFDAQTWLSSALTSIDTCLSGAADLNVTDFIAPI-RFQNVSEMISNCLAINGAFL 120
            K CTDFDAQTWLS+ALT+ +TC  G++DLNVTDFI PI     +S +ISNCLA+NGA L
Sbjct: 129 AKSCTDFDAQTWLSTALTNTETCRRGSSDLNVTDFITPIVSNTKISHLISNCLAVNGALL 188

Query: 121 GDGKNG--TEEENGFPMWVSSGDRKLLQSSSTKVRANLVVARDGSGNFRTVQAAINAAAR 180
             G  G  T  + GFP W+S  D++LL++    VRANLVVA+DGSG+F TVQAAI+ A R
Sbjct: 189 TAGNKGNTTANQKGFPTWLSRKDKRLLRA----VRANLVVAKDGSGHFNTVQAAIDVAGR 248

Query: 181 RR-GRGRFVIYVKRGVYRENIEVENDNGNLMLVGDGMRFTVITSGRSVAAGFTTFSSATA 240
           R+   GRFVIYVKRG+Y+ENI V  +N ++MLVGDGMR T+IT GRSV  G+TT++SATA
Sbjct: 249 RKVTSGRFVIYVKRGIYQENINVRLNNDDIMLVGDGMRSTIITGGRSVQGGYTTYNSATA 308

Query: 241 GIEGPAFIARDITFVNTAGPRMGQAVALRSSSDLSVFHRCGFQGYQDTLMVLSQRQFYKQ 300
           GIEG  FIA+ ITF NTAGP  GQAVALRSSSDLS+F++C  +GYQDTLMV SQRQFY++
Sbjct: 309 GIEGLHFIAKGITFRNTAGPAKGQAVALRSSSDLSIFYKCSIEGYQDTLMVHSQRQFYRE 368

Query: 301 CYVYGTIDFIFGNAAVVLQNCMIYVRRPLKGQVNVITAQGREDPYQNSGISIHNSQIRAA 360
           CY+YGT+DFIFGNAA V QNC+I  RRPLKGQ NVITAQGR DP+QN+GISIHNS+I  A
Sbjct: 369 CYIYGTVDFIFGNAAAVFQNCLILPRRPLKGQANVITAQGRADPFQNTGISIHNSRILPA 428

Query: 361 PDLRPVIGSVKTYLGRPWKKYSRTVIMRSYIDSLVSPMGWLAW-QSSKFAQDTLYYGEFK 420
           PDL+PV+G+VKTY+GRPW K+SRTV++++Y+D++VSP+GW  W + S F  DTL+Y E+K
Sbjct: 429 PDLKPVVGTVKTYMGRPWMKFSRTVVLQTYLDNVVSPVGWSPWIEGSVFGLDTLFYAEYK 488

Query: 421 NFGPRASTRYRVRWPGFHVIRSPNVASQFTVRRLIAGQTWLPATGVPFTLGL 463
           N GP +STR+RV W GFHV+   + AS FTV + IAG  WLP TG+PFT GL
Sbjct: 489 NTGPASSTRWRVSWKGFHVLGRASDASAFTVGKFIAGTAWLPRTGIPFTSGL 536

[Close]

BLAST of Moc10g02350 vs. TAIR 10
Match: AT5G51500.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 576.2 bits (1484), Expect = 2.3e-164
Identity = 288/467 (61.67%), Postives = 360/467 (77.09%), Query Frame = 0


Query: 1   MAIQTALEKAAEAQHYATRFGQSCKNTRQRAAWADCLKLYHDAVFQLNRTLQGLSRK--- 60
           + ++ A+++A  A    T   ++C + +++A  ADC+ LY D V QLNRTLQG+S K   
Sbjct: 73  LLVEAAMDRAVSAWDKLTNSSKNCTDFKKQAVLADCINLYGDTVMQLNRTLQGVSSKTGR 132

Query: 61  GCTDFDAQTWLSSALTSIDTCLSGAADLNVTDFIAPI-RFQNVSEMISNCLAINGAFLGD 120
            CTDFDAQTWLS+ALT+ +TC  G++DLNV+DF  PI     +S +ISNCLA+NGA L  
Sbjct: 133 RCTDFDAQTWLSTALTNTETCRRGSSDLNVSDFTTPIVSNTKISHLISNCLAVNGALLTA 192

Query: 121 GKNG--TEEENGFPMWVSSGDRKLLQSSSTKVRANLVVARDGSGNFRTVQAAINAAARRR 180
           GKN   T +  GFP WVS  +R+LLQ  S  VRANLVVA+DGSG+F+TVQAAI+ A RR+
Sbjct: 193 GKNDSTTGDSKGFPTWVSRKERRLLQLQS--VRANLVVAKDGSGHFKTVQAAIDVAGRRK 252

Query: 181 -GRGRFVIYVKRGVYRENIEVENDNGNLMLVGDGMRFTVITSGRSVAAGFTTFSSATAGI 240
              GRFVIYVKRG+Y+EN+ V  +N N+MLVGDGMR+T+IT GRSV  G+TT+SSATAGI
Sbjct: 253 VTSGRFVIYVKRGIYQENLNVRLNNDNIMLVGDGMRYTIITGGRSVKGGYTTYSSATAGI 312

Query: 241 EGPAFIARDITFVNTAGPRMGQAVALRSSSDLSVFHRCGFQGYQDTLMVLSQRQFYKQCY 300
           EG  FIA+ I F NTAGP  GQAVALRSSSDLS+F+RC  +GYQDTLMV SQRQFY++CY
Sbjct: 313 EGLHFIAKGIAFQNTAGPAKGQAVALRSSSDLSIFYRCSIEGYQDTLMVHSQRQFYRECY 372

Query: 301 VYGTIDFIFGNAAVVLQNCMIYVRRPLKGQVNVITAQGREDPYQNSGISIHNSQIRAAPD 360
           +YGT+DFIFGNAAVV QNC+I  R PLKGQ NVITAQGR D +QN+GISIHNS I  APD
Sbjct: 373 IYGTVDFIFGNAAVVFQNCIILPRLPLKGQANVITAQGRTDLFQNTGISIHNSIIIPAPD 432

Query: 361 LRPVIGSVKTYLGRPWKKYSRTVIMRSYIDSLVSPMGWLAW-QSSKFAQDTLYYGEFKNF 420
           L+PV+ SVKTY+GRPW  YSRTV++++YIDS+VSP+GW  W + S +  DTL+Y E+KN 
Sbjct: 433 LKPVVRSVKTYMGRPWMMYSRTVVLKTYIDSVVSPVGWSPWTKGSTYGLDTLFYAEYKNI 492

Query: 421 GPRASTRYRVRWPGFHVIRSPNVASQFTVRRLIAGQTWLPATGVPFT 460
           GP +STR+RVRW GFHV+   + AS F+V + IAG  WLP +G+PFT
Sbjct: 493 GPASSTRWRVRWKGFHVLSKASDASAFSVGKFIAGTAWLPGSGIPFT 537

[Close]

BLAST of Moc10g02350 vs. TAIR 10
Match: AT3G47400.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 570.5 bits (1469), Expect = 1.3e-162
Identity = 286/472 (60.59%), Postives = 363/472 (76.91%), Query Frame = 0


Query: 1   MAIQTALEKAAEAQHYATRFGQSCKNTRQRAAWADCLKLYHDAVFQLNRTLQGL-----S 60
           M ++ AL++A        +FG SC N +++AAW+DC+ L+ + V QLNRTL+GL     S
Sbjct: 123 MLVKVALDQAVITHSQTVKFGPSCTNNQRKAAWSDCVNLFQNTVAQLNRTLKGLNPAASS 182

Query: 61  RKGCTDFDAQTWLSSALTSIDTCLSGAADLNVTDFIAP-IRFQNVSEMISNCLAINGAFL 120
              CTDFDAQTWLS+A T+I+TC SG+ DLNV+DF+ P I  +N+S++I NCLA+NG  +
Sbjct: 183 DVKCTDFDAQTWLSTAQTNIETCRSGSEDLNVSDFVMPVISNKNLSDLIGNCLAVNGVLM 242

Query: 121 --GDGKNGTEEENGFPMWVSSGDRKLLQSSS-TKVRANLVVARDGSGNFRTVQAAINAAA 180
              D       +  FP WVS  +R+LL S+S  K   +LVVA+D SG+FR++QAAIN AA
Sbjct: 243 KQHDHTTTANHKEYFPSWVSRHERRLLVSASLAKSSPHLVVAQDRSGHFRSIQAAINFAA 302

Query: 181 RRRGRGRFVIYVKRGVYRENIEVENDNGNLMLVGDGMRFTVITSGRSVAAGFTTFSSATA 240
           RRR + RFVIYVK+GVYRENI+V NDN N+MLVGDG R T+ITSGRSV  G+TT++SAT 
Sbjct: 303 RRRFKSRFVIYVKKGVYRENIDVGNDNHNIMLVGDGERKTIITSGRSVQHGYTTYNSATG 362

Query: 241 GIEGPAFIARDITFVNTAGPRMGQAVALRSSSDLSVFHRCGFQGYQDTLMVLSQRQFYKQ 300
           G  G  F+A+D+TF+NTAGP  GQAVA+RSSSDLSVF+R G  G+QDTL + SQRQF+++
Sbjct: 363 GFGGQRFVAKDMTFINTAGPLRGQAVAVRSSSDLSVFYRVGIHGFQDTLYIHSQRQFFRE 422

Query: 301 CYVYGTIDFIFGNAAVVLQNCMIYVRRPLKGQVNVITAQGREDPYQNSGISIHNSQIRAA 360
           CY+ GTIDFIFGNAAVV QNCMI VRRPL GQ N+ITAQGR DP+QN+GI+IH+S+I AA
Sbjct: 423 CYISGTIDFIFGNAAVVFQNCMILVRRPLHGQANIITAQGRGDPFQNTGITIHSSRIIAA 482

Query: 361 PDLRPVIGSVKTYLGRPWKKYSRTVIMRSYIDSLVSPMGWLAW-QSSKFAQDTLYYGEFK 420
            DL+PVI + KTYLGRPW+ YSR  IM++YID+ +SP+GW  W + S FA +T++YGE+K
Sbjct: 483 SDLKPVIRAYKTYLGRPWQAYSRVTIMKTYIDNSISPLGWSPWLRGSNFALNTVFYGEYK 542

Query: 421 NFGPRASTRYRVRWPGFHVIRSPNVASQFTVRRLIAGQTWLPATGVPFTLGL 463
           NFGP +STR+RVRW GFH I S  VAS+FTV  LIAG +WLPATGVPF  GL
Sbjct: 543 NFGPGSSTRWRVRWKGFHAITSTAVASRFTVGSLIAGGSWLPATGVPFKSGL 594

[Close]

BLAST of Moc10g02350 vs. TAIR 10
Match: AT2G45220.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 487.6 bits (1254), Expect = 1.1e-137
Identity = 255/462 (55.19%), Postives = 327/462 (70.78%), Query Frame = 0


Query: 1   MAIQTALEKAAEAQHYATRFGQSCKNTRQRAAWADCLKLYHDAVFQLNRTLQGLSRKGCT 60
           ++++  L++A  A+ +A   G  C++TR++AAW DC+KLY   V ++N T+       C+
Sbjct: 65  ISMKLVLDRAILAKTHAFTLGPKCRDTREKAAWEDCIKLYDLTVSKINETMD--PNVKCS 124

Query: 61  DFDAQTWLSSALTSIDTCLSGAADLNVTDFIAPIRFQNVSEMISNCLAINGAFLGDGKNG 120
             DAQTWLS+ALT++DTC +G  +L VTD + P+   NVS ++ N LAIN          
Sbjct: 125 KLDAQTWLSTALTNLDTCRAGFLELGVTDIVLPLMSNNVSNLLCNTLAINKV---PFNYT 184

Query: 121 TEEENGFPMWVSSGDRKLLQSSSTKVRANLVVARDGSGNFRTVQAAINAAARRRGRGRFV 180
             E++GFP WV  GDRKLLQSS+ K   N VVA+DGSGNF+T++ AI+AA+   G GRFV
Sbjct: 185 PPEKDGFPSWVKPGDRKLLQSSTPK--DNAVVAKDGSGNFKTIKEAIDAAS---GSGRFV 244

Query: 181 IYVKRGVYRENIEVENDNGNLMLVGDGMRFTVITSGRSVAAGFTTFSSATAGIEGPAFIA 240
           IYVK+GVY EN+E+     N+ML GDG+  T+IT  +SV  G TTF+SAT    G  FIA
Sbjct: 245 IYVKQGVYSENLEIRKK--NVMLRGDGIGKTIITGSKSVGGGTTTFNSATVAAVGDGFIA 304

Query: 241 RDITFVNTAGPRMGQAVALRSSSDLSVFHRCGFQGYQDTLMVLSQRQFYKQCYVYGTIDF 300
           R ITF NTAG    QAVALRS SDLSVF++C F+ YQDTL V S RQFY+ C VYGT+DF
Sbjct: 305 RGITFRNTAGASNEQAVALRSGSDLSVFYQCSFEAYQDTLYVHSNRQFYRDCDVYGTVDF 364

Query: 301 IFGNAAVVLQNCMIYVRRPLKGQVNVITAQGREDPYQNSGISIHNSQIRAAPDLRPVIGS 360
           IFGNAA VLQNC I+ RRP + + N ITAQGR DP QN+GI IHNS++ AA DLRPV+GS
Sbjct: 365 IFGNAAAVLQNCNIFARRP-RSKTNTITAQGRSDPNQNTGIIIHNSRVTAASDLRPVLGS 424

Query: 361 VKTYLGRPWKKYSRTVIMRSYIDSLVSPMGWLAWQSSKFAQDTLYYGEFKNFGPRASTRY 420
            KTYLGRPW++YSRTV M++ +DSL+ P GWL W  + FA  TL+Y EF+N GP AST  
Sbjct: 425 TKTYLGRPWRQYSRTVFMKTSLDSLIDPRGWLEWDGN-FALKTLFYAEFQNTGPGASTSG 484

Query: 421 RVRWPGFHVIRSPNVASQFTVRRLIAGQTWLPATGVPFTLGL 463
           RV WPGF V+ S + AS+FTV   +AG +W+P++ VPFT GL
Sbjct: 485 RVTWPGFRVLGSASEASKFTVGTFLAGGSWIPSS-VPFTSGL 511

[Close]

BLAST of Moc10g02350 vs. TAIR 10
Match: AT4G02320.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 395.6 bits (1015), Expect = 5.5e-110
Identity = 216/449 (48.11%), Postives = 285/449 (63.47%), Query Frame = 0


Query: 28  RQRAAWADCLKLYHDAVFQLNRTLQGLSRKGCTDFDAQTWLSSALTSIDTCLSGAA---- 87
           R   A+ DCL+L  D VF L   +  L        + +  LS+A+T+  TCL G A    
Sbjct: 73  RDLCAFDDCLELLDDTVFDLTTAISKLRSHSPELHNVKMLLSAAMTNTRTCLDGFASSDN 132

Query: 88  --DLN--------VTDFIAPIRFQNVSEMISNCLAINGAFLGDGKNGTEEENGFPMWVSS 147
             +LN        V + +    F N+S  +S+ LA+     G      +E+ GFPMWVS 
Sbjct: 133 DENLNNNDNKTYGVAESLKESLF-NISSHVSDSLAMLENIPGHIPGKVKEDVGFPMWVSG 192

Query: 148 GDRKLLQSSSTKVRANLVVARDGSGNFRTVQAAINAAARRRGRGRFVIYVKRGVYRENIE 207
            DR LLQ    + + NLVVA++G+GN+ T+  AI+AA       RFVIY+K G Y ENIE
Sbjct: 193 SDRNLLQDPVDETKVNLVVAQNGTGNYTTIGEAISAAP-NSSETRFVIYIKCGEYFENIE 252

Query: 208 VENDNGNLMLVGDGMRFTVITSGRSVAAGFTTFSSATAGIEGPAFIARDITFVNTAGPRM 267
           +  +   +M +GDG+  TVI + RS A G+T F SAT G+ G  FIA+D++FVN AGP  
Sbjct: 253 IPREKTMIMFIGDGIGRTVIKANRSYADGWTAFHSATVGVRGSGFIAKDLSFVNYAGPEK 312

Query: 268 GQAVALRSSSDLSVFHRCGFQGYQDTLMVLSQRQFYKQCYVYGTIDFIFGNAAVVLQNCM 327
            QAVALRSSSDLS ++RC F+ YQDT+ V S +QFY++C +YGT+DFIFG+A+VV QNC 
Sbjct: 313 HQAVALRSSSDLSAYYRCSFESYQDTIYVHSHKQFYRECDIYGTVDFIFGDASVVFQNCS 372

Query: 328 IYVRRPLKGQVNVITAQGREDPYQNSGISIHNSQIRAAPDLRPVIGSVKTYLGRPWKKYS 387
           +Y RRP   Q  + TAQGRE+  + +GISI +S+I AAPDL PV  + K YLGRPW+ YS
Sbjct: 373 LYARRPNPNQKIIYTAQGRENSREPTGISIISSRILAAPDLIPVQANFKAYLGRPWQLYS 432

Query: 388 RTVIMRSYIDSLVSPMGWLAWQSSKFAQDTLYYGEFKNFGPRASTRYRVRWPGFHVIRSP 447
           RTVIM+S+ID LV P GWL W+   FA +TLYYGE+ N GP ++   RV+WPGF  I + 
Sbjct: 433 RTVIMKSFIDDLVDPAGWLKWKDD-FALETLYYGEYMNEGPGSNMTNRVQWPGFKRIETV 492

Query: 448 NVASQFTVRRLIAGQTWLPATGVPFTLGL 463
             ASQF+V   I G  WL +T +PFTL L
Sbjct: 493 EEASQFSVGPFIDGNKWLNSTRIPFTLDL 518

The following BLAST results are available for this feature:

BLAST of Moc10g02350 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038905752.1	1.8e-224	84.03	probable pectinesterase/pectinesterase inhibitor 60 [Benincasa hispida]	[more]
XP_008452338.1	1.6e-220	82.35	PREDICTED: probable pectinesterase/pectinesterase inhibitor 59 [Cucumis melo] >X...	[more]
XP_011650542.1	2.8e-220	81.68	probable pectinesterase/pectinesterase inhibitor 59 [Cucumis sativus] >KGN56178....	[more]
KAG7015521.1	2.8e-217	81.74	putative pectinesterase/pectinesterase inhibitor 33, partial [Cucurbita argyrosp...	[more]
XP_022985199.1	3.7e-217	81.53	probable pectinesterase/pectinesterase inhibitor 33 [Cucurbita maxima]	[more]

BLAST of Moc10g02350 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FHN5	1.5e-165	61.02	Probable pectinesterase/pectinesterase inhibitor 59 OS=Arabidopsis thaliana OX=3...	[more]
Q9FHN4	3.2e-163	61.67	Probable pectinesterase/pectinesterase inhibitor 60 OS=Arabidopsis thaliana OX=3...	[more]
Q9STY3	1.8e-161	60.59	Probable pectinesterase/pectinesterase inhibitor 33 OS=Arabidopsis thaliana OX=3...	[more]
O04887	2.2e-140	55.41	Pectinesterase 2 OS=Citrus sinensis OX=2711 GN=PECS-2.1 PE=2 SV=1	[more]
O22149	1.5e-136	55.19	Probable pectinesterase/pectinesterase inhibitor 17 OS=Arabidopsis thaliana OX=3...	[more]

BLAST of Moc10g02350 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3BUR4	7.8e-221	82.35	Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103493398 PE=3 SV=1	[more]
A0A0A0L330	1.3e-220	81.68	Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_3G088980 PE=3 SV=1	[more]
A0A6J1JCM7	1.8e-217	81.53	Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111483275 PE=3 SV=1	[more]
A0A6J1EW59	5.2e-217	81.53	Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111438452 PE=3 SV=1	[more]
A0A5A7TVK4	1.2e-216	81.09	Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold68G001080...	[more]

BLAST of Moc10g02350 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G51490.1	1.1e-166	61.02	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT5G51500.1	2.3e-164	61.67	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT3G47400.1	1.3e-162	60.59	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT2G45220.1	1.1e-137	55.19	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT4G02320.1	5.5e-110	48.11	Plant invertase/pectin methylesterase inhibitor superfamily	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006501	Pectinesterase inhibitor domain	SMART	SM00856	PMEI_2	coord: 1..109 e-value: 5.0E-13 score: 59.3
IPR006501	Pectinesterase inhibitor domain	TIGRFAM	TIGR01614	TIGR01614	coord: 1..110 e-value: 3.1E-15 score: 53.9
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 1..109 e-value: 1.2E-17 score: 64.7
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	GENE3D	1.20.140.40	Invertase/pectin methylesterase inhibitor family protein	coord: 1..113 e-value: 5.3E-22 score: 80.4
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	SUPERFAMILY	101148	Plant invertase/pectin methylesterase inhibitor	coord: 1..113
IPR000070	Pectinesterase, catalytic	PFAM	PF01095	Pectinesterase	coord: 149..447 e-value: 3.3E-129 score: 429.9
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 143..462 e-value: 7.6E-112 score: 375.8
None	No IPR available	PANTHER	PTHR31707:SF45	PECTINESTERASE/PECTINESTERASE INHIBITOR 59-RELATED	coord: 1..461
None	No IPR available	PANTHER	PTHR31707	PECTINESTERASE	coord: 1..461
None	No IPR available	CDD	cd15798	PMEI-like_3	coord: 1..113 e-value: 4.74782E-27 score: 104.057
IPR018040	Pectinesterase, Tyr active site	PROSITE	PS00800	PECTINESTERASE_1	coord: 175..194
IPR033131	Pectinesterase, Asp active site	PROSITE	PS00503	PECTINESTERASE_2	coord: 294..303
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 145..461

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc10g02350.1	Moc10g02350.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0042545	cell wall modification
biological_process	GO:0043086	negative regulation of catalytic activity
biological_process	GO:0045490	pectin catabolic process
cellular_component	GO:0005576	extracellular region
molecular_function	GO:0045330	aspartyl esterase activity
molecular_function	GO:0030599	pectinesterase activity
molecular_function	GO:0046910	pectinesterase inhibitor activity
molecular_function	GO:0004857	enzyme inhibitor activity