Moc09g31960 (gene) Bitter gourd (OHB3-1) v2

BLAST of Moc09g31960 vs. NCBI nr
Match: XP_022137317.1 (uncharacterized protein LOC111008813 [Momordica charantia])

HSP 1 Score: 951.0 bits (2457), Expect = 7.5e-273
Identity = 489/528 (92.61%), Postives = 504/528 (95.45%), Query Frame = 0

Query: 191 QAESSHN---PAGIITREEFDQLRGKLDAQVEALKAKCEQKDDSLNEGDFRESPFTSDVL 250
           +AESS N   PAG+ITREEFDQLRG+LDAQVEALKAKCEQK+  LN+GD  ESPFTSDVL
Sbjct: 3   KAESSRNPATPAGVITREEFDQLRGQLDAQVEALKAKCEQKEGPLNDGDLGESPFTSDVL 62

Query: 251 EAPIPPKFKAPTMKPYDGTKDPKDYVEVFEGLMDFQAASDAIKCRAFQIALTGSARLWYR 310
           EAPIPPKFKAPT+KPYDG+KDPKDYVEVFE LMDFQAASDAIKCRAF+IALTGSARLWYR
Sbjct: 63  EAPIPPKFKAPTVKPYDGSKDPKDYVEVFESLMDFQAASDAIKCRAFRIALTGSARLWYR 122

Query: 311 RLPARSISTYSQLRREFLAQFSSRHYDKKTTTHLATIRQKEGETLREYVTRFQEEQLKVA 370
           RLPA SISTYSQLRREFLA FSSRHYDKKT THLATIRQKEGETLREYVTRFQEEQLKVA
Sbjct: 123 RLPAXSISTYSQLRREFLAXFSSRHYDKKTATHLATIRQKEGETLREYVTRFQEEQLKVA 182

Query: 371 HCSDDSAMCYFLTGLADEALTVKLGEEAPATFAEVLQKAKKVIDGQELLRTKTGRPERKI 430
           HCSDDSAMCYFLTGLADEALTVKLGEEAPATFAEVLQKAKKVIDGQELLRTKTGRPERKI
Sbjct: 183 HCSDDSAMCYFLTGLADEALTVKLGEEAPATFAEVLQKAKKVIDGQELLRTKTGRPERKI 242

Query: 431 GRGRSGKDVERADPKSKDKESFSSGRAEYRRAESGPTRSRPYERFTPTTIPISEILTNIE 490
           GRGRSGKD+E ADPKSKDK SFSSGRAEYRRAE+GPTRSRPYERFTPTTIPISEILTNIE
Sbjct: 243 GRGRSGKDIENADPKSKDKGSFSSGRAEYRRAENGPTRSRPYERFTPTTIPISEILTNIE 302

Query: 491 ESGMEKLLKRPEKLRRAPERRSKDKYYRFHREHDHNTSDCWELKRQIEDLIQDGYFKKFV 550
           ESGMEKLLKRPEKLR APERRSKDKY RFHREH HNTSD WELKRQIE+LIQDGYFKKFV
Sbjct: 303 ESGMEKLLKRPEKLRGAPERRSKDKYCRFHREHGHNTSDYWELKRQIENLIQDGYFKKFV 362

Query: 551 GKPRTSSAEKKKERKRSRTPPRRTDRPAVINTIFGGPSGGQSGHKRKELARAARREVCII 610
           GKPRTSSAEKK+ERKRSRTPPRRTDRPAVINTIFGGPSGGQSG KRKELARAARREVCII
Sbjct: 363 GKPRTSSAEKKEERKRSRTPPRRTDRPAVINTIFGGPSGGQSGRKRKELARAARREVCII 422

Query: 611 REQGPTCPITFNGADLEEVHLPHNDALVIAPLIDHVVVRRVLVDGGASANILSLPTYFAL 670
           REQ PTCPITF+GADLEEVHLPHNDALVIAPLIDHVVV RVLVDGG SANILSLPTY AL
Sbjct: 423 REQRPTCPITFDGADLEEVHLPHNDALVIAPLIDHVVVXRVLVDGGTSANILSLPTYLAL 482

Query: 671 GWTRSQLKKSPTPLVGFSGESVIPEGCIDLPVTLGQDRTRVTQMAEFV 716
           GWTRSQLKKSPTPLVGFSGESVIPEG IDLPVTLGQD+T+VTQMAEFV
Sbjct: 483 GWTRSQLKKSPTPLVGFSGESVIPEGFIDLPVTLGQDQTQVTQMAEFV 530

BLAST of Moc09g31960 vs. NCBI nr
Match: XP_022150760.1 (uncharacterized protein LOC111018823 [Momordica charantia])

HSP 1 Score: 941.4 bits (2432), Expect = 5.9e-270
Identity = 513/665 (77.14%), Postives = 542/665 (81.50%), Query Frame = 0

Query: 187 SSNQQAESSHNPA---GIITREEFDQLRGKLDAQVEALKAKCEQKDDSLNEGDFRESPFT 246
           SSNQQAESSHNPA   G+ITREEFDQLRGKL+AQVEALKAKCEQK+  LN+GD  ESPFT
Sbjct: 2   SSNQQAESSHNPATPDGVITREEFDQLRGKLNAQVEALKAKCEQKEGPLNDGDLGESPFT 61

Query: 247 SDVLEAPIPPKFKAPTMKPYDGTKDPKDYVEVFEGLMDFQAASDAIKCRAFQIALTGSAR 306
           SDVLE        APT+K YDG+KDPKDYVEVFEGLMDFQAASDAIKCRAFQIALTGSAR
Sbjct: 62  SDVLE--------APTVKSYDGSKDPKDYVEVFEGLMDFQAASDAIKCRAFQIALTGSAR 121

Query: 307 LWYRRLPARSISTYSQLRREFLAQFSSRHYDKKTTTHLATIRQKEGETLREYVTRFQEEQ 366
           LW                                                     FQE+Q
Sbjct: 122 LW-----------------------------------------------------FQEDQ 181

Query: 367 LKVAHCSDDSAMCYFLTGLADEALTVKLGEEAPATFAEVLQKAKKVIDGQELLRTKTGRP 426
           LKVA  SDDSAMCYFLTGLADEALTVKLG+EAPATFAEVLQKAKKVIDGQELLRTKTGRP
Sbjct: 182 LKVAQSSDDSAMCYFLTGLADEALTVKLGKEAPATFAEVLQKAKKVIDGQELLRTKTGRP 241

Query: 427 ERKIGRGRSGKDVERADPKSKDKESFSSGRAEYRRAESGPTRSRPYERFTPTTIPISEIL 486
           ER I RGRSGKD E+AD KSKDK SFSSGRAE+RRA +GPTRSRPYERFTPTTIPISEIL
Sbjct: 242 ERGIDRGRSGKD-EKADLKSKDKGSFSSGRAEFRRAVNGPTRSRPYERFTPTTIPISEIL 301

Query: 487 TNIEESGMEKLLKRPEKLRRAPERRSKDKYYRFHREHDHNTSDCWELKRQIEDLIQDGYF 546
           TNIEESGMEKLLKRPEKLR APERR+KDKY RFHREHDHNTSD WELKRQIEDLIQD YF
Sbjct: 302 TNIEESGMEKLLKRPEKLRGAPERRNKDKYCRFHREHDHNTSDRWELKRQIEDLIQDDYF 361

Query: 547 KKFVGKPRTSSAEKKKERKRSRTPPRRTDRPAVINTIFGGPSGGQSGHKRKELARAARRE 606
           KKFVGKPRTSSAEKK+ERK SRTP RR DRPAVINTIFGGPSGGQSGHKRKELARAARRE
Sbjct: 362 KKFVGKPRTSSAEKKEERKLSRTPLRRIDRPAVINTIFGGPSGGQSGHKRKELARAARRE 421

Query: 607 VCIIREQGPTCPITFNGADLEEVHLPHNDALVIAPLIDHVVVRRVLVDGGASANILSLPT 666
           VCIIREQ PTCPITF+ ADLEEVHLPHNDALVIAPLIDHVVVRRVLVD G SANI+SL T
Sbjct: 422 VCIIREQRPTCPITFDSADLEEVHLPHNDALVIAPLIDHVVVRRVLVDEGVSANIVSLLT 481

Query: 667 YFALGWTRSQLKKSPTPLVGFSGESVIPEGCIDLPVTLGQDRTRVTQMAEFVVIDGRSAY 726
           Y ALGWTRSQLKKS TPLVGFS ESVIPEGCIDLPVTLG D+T+VTQMAEFVVIDGRSAY
Sbjct: 482 YLALGWTRSQLKKSTTPLVGFSRESVIPEGCIDLPVTLGHDQTQVTQMAEFVVIDGRSAY 541

Query: 727 NVIFGRPIIHSFRAIPSTLHQVLKYPTLNGVGTVRGEQTASREWYASALKGSSVCALETL 786
           N IFGRPIIHSFRAIPSTLHQVLKY T NGVG VRGEQ ASRE YASALKGSSVCALETL
Sbjct: 542 NAIFGRPIIHSFRAIPSTLHQVLKYSTPNGVGMVRGEQIASRECYASALKGSSVCALETL 601

Query: 787 PGRDGTLEFEANPPRKEFAAPTEELELVSLLSLEKQTDLARSVPIEILDNPSILEPDLME 846
             RDGTLEF+AN PR+EFAAPTEELELV LL  +   ++     ++   + + ++ D+  
Sbjct: 602 VSRDGTLEFKANLPRREFAAPTEELELVPLLRYKYNENIDHEQELDEKSSLNKIDDDIGV 604

Query: 847 IGAPE 849
            G PE
Sbjct: 662 EGMPE 604

BLAST of Moc09g31960 vs. NCBI nr
Match: XP_022152854.1 (uncharacterized protein LOC111020479 [Momordica charantia])

HSP 1 Score: 940.6 bits (2430), Expect = 1.0e-269
Identity = 522/790 (66.08%), Postives = 566/790 (71.65%), Query Frame = 0

Query: 1   MVQPANSTNTADRRTLAASDAHQREVGAAAVEGQGHDGLATEPLRRSARITAPALPPAHP 60
           MVQPANSTNTADRR LAA+  HQREVGA  VEGQGH+ L TEPL RSARIT P LPPAHP
Sbjct: 1   MVQPANSTNTADRRALAANHGHQREVGAEVVEGQGHEDLGTEPLCRSARITTPVLPPAHP 60

Query: 61  RTSKATRGRGGTSKKGARGPAPAPTSENFDALQREMEAMRTQMRSMEEMYNEMMLAAGAG 120
           + SK                                                        
Sbjct: 61  KPSK-------------------------------------------------------- 120

Query: 121 SRSENRVTRVDIREQRGSHLGPAEEERPEDNESEGYTRQRGDLREHLNRKRGSSLRKGQS 180
                                                                       
Sbjct: 121 ------------------------------------------------------------ 180

Query: 181 PSRSHRSSNQQAESSHNP--AGIITREEFDQLRGKLDAQVEALKAKCEQKDDSLNEGDFR 240
                      AESS+NP   G+ITREEFDQL+ K DAQVEALKA+CE+K+ S ++GD  
Sbjct: 181 -----------AESSYNPITPGVITREEFDQLKSKFDAQVEALKARCEKKESSFDDGDLG 240

Query: 241 ESPFTSDVLEAPIPPKFKAPTMKPYDGTKDPKDYVEVFEGLMDFQAASDAIKCRAFQIAL 300
           E  F+SD+LEA IPPKFK PTMKPYDG+KDPKDYVEVFE LMDFQAA+DAIKC AFQIAL
Sbjct: 241 ELSFSSDILEALIPPKFKTPTMKPYDGSKDPKDYVEVFESLMDFQAATDAIKCCAFQIAL 300

Query: 301 TGSARLWYRRLPARSISTYSQLRREFLAQFSSRHYDKKTTTHLATIRQKEGETLREYVTR 360
           TGSARLWYRRLPAR ISTYSQLR+EF++QFSSRHYD+KT THLATIRQKEGETLREYVTR
Sbjct: 301 TGSARLWYRRLPARLISTYSQLRKEFISQFSSRHYDRKTPTHLATIRQKEGETLREYVTR 360

Query: 361 FQEEQLKVAHCSDDSAMCYFLTGLADEALTVKLGEEAPATFAEVLQKAKKVIDGQELLRT 420
           F EEQLKVAHCSDDSAMCYFLTGLADE LTVKL EEAPATFAEVLQK KKVIDGQELLRT
Sbjct: 361 FPEEQLKVAHCSDDSAMCYFLTGLADETLTVKLREEAPATFAEVLQKTKKVIDGQELLRT 420

Query: 421 KTGRPERKIGRGRSGKDVERADPKSKDK-ESFSSGRAEYRRAESGPTRSRPYERFTPTTI 480
           KTGRPE+ I +GR+GKD  +AD KS+DK  S SS R +YRR+ S   +SRPYE +TPTTI
Sbjct: 421 KTGRPEKNIDQGRAGKDKGKADSKSRDKGPSSSSSRVDYRRSNSSHNQSRPYEHYTPTTI 480

Query: 481 PISEILTNIEESGMEKLLKRPEKLRRAPERRSKDKYYRFHREHDHNTSDCWELKRQIEDL 540
           PI EILTNIEE+GMEKLLKRPEKLR  PE+R+ DKY RFHR+H HNTS+ WELKRQIEDL
Sbjct: 481 PIFEILTNIEETGMEKLLKRPEKLRGDPEKRNTDKYCRFHRDHGHNTSNYWELKRQIEDL 540

Query: 541 IQDGYFKKFVGKPRTSSAEKKKERKRSRTPPRRTDRPAVINTIFGGPSGGQSGHKRKELA 600
           IQDGYFKKFVGKPR++S EKK+ERKR RTPPRR DRPAVIN             K+KELA
Sbjct: 541 IQDGYFKKFVGKPRSNSVEKKEERKRLRTPPRRDDRPAVIN-------------KKKELA 600

Query: 601 RAARREVCIIREQGPTCPITFNGADLEEVHLPHNDALVIAPLIDHVVVRRVLVDGGASAN 660
           R ARREVCIIREQ PT  I FN ADLE VHLPHNDALVIAPLID V+VRR+LVDGGASAN
Sbjct: 601 REARREVCIIREQRPTSSIAFNHADLEGVHLPHNDALVIAPLIDLVLVRRILVDGGASAN 650

Query: 661 ILSLPTYFALGWTRSQLKKSPTPLVGFSGESVIPEGCIDLPVTLGQDRTRVTQMAEFVVI 720
           ILSL TY ALGWTRSQLKKSPTPLVGFSGES+  EGCIDLPV++ QD T+VTQMAEFVVI
Sbjct: 661 ILSLSTYLALGWTRSQLKKSPTPLVGFSGESISLEGCIDLPVSIRQDDTQVTQMAEFVVI 650

Query: 721 DGRSAYNVIFGRPIIHSFRAIPSTLHQVLKYPTLNGVGTVRGEQTASREWYASALKGSSV 780
           DGRSAYN IFGRPIIHSFRA+PSTLHQVLKY TLNGVGTVRGE   SRE YAS  K SSV
Sbjct: 721 DGRSAYNAIFGRPIIHSFRAVPSTLHQVLKYSTLNGVGTVRGELKTSRECYASVPKRSSV 650

Query: 781 CALETLPGRD 788
           CALE    RD
Sbjct: 781 CALEEQTIRD 650

BLAST of Moc09g31960 vs. NCBI nr
Match: XP_022150613.1 (uncharacterized protein LOC111018708, partial [Momordica charantia])

HSP 1 Score: 779.6 bits (2012), Expect = 3.0e-221
Identity = 399/422 (94.55%), Postives = 407/422 (96.45%), Query Frame = 0

Query: 228 KDDSLNEGDFRESPFTSDVLEAPIPPKFKAPTMKPYDGTKDPKDYVEVFEGLMDFQAASD 287
           KDDSLN+GD  ES FTSDVLEAPIPPKFKAPT+KPYDG+KDPKDYVEVFEGLMDF AASD
Sbjct: 44  KDDSLNDGDLGESSFTSDVLEAPIPPKFKAPTVKPYDGSKDPKDYVEVFEGLMDFHAASD 103

Query: 288 AIKCRAFQIALTGSARLWYRRLPARSISTYSQLRREFLAQFSSRHYDKKTTTHLATIRQK 347
           AIKCRAFQIALTGSARLWYRRLPARSISTYSQLRREFLAQFSSR Y KKT THLATIRQK
Sbjct: 104 AIKCRAFQIALTGSARLWYRRLPARSISTYSQLRREFLAQFSSRQYGKKTETHLATIRQK 163

Query: 348 EGETLREYVTRFQEEQLKVAHCSDDSAMCYFLTGLADEALTVKLGEEAPATFAEVLQKAK 407
           EG TLREYVTRFQEEQLKVAHCSDDSAMCYFLTGLADEALTVKLGE+AP TFAEVLQKAK
Sbjct: 164 EGGTLREYVTRFQEEQLKVAHCSDDSAMCYFLTGLADEALTVKLGEKAPTTFAEVLQKAK 223

Query: 408 KVIDGQELLRTKTGRPERKIGRGRSGKDVERADPKSKDKESFSSGRAEYRRAESGPTRSR 467
           KVIDGQELLRTKTGRP+RKIGRGRSGKDVERADPKSKDK SFSSGRAEYRRAESGPT+SR
Sbjct: 224 KVIDGQELLRTKTGRPDRKIGRGRSGKDVERADPKSKDKGSFSSGRAEYRRAESGPTKSR 283

Query: 468 PYERFTPTTIPISEILTNIEESGMEKLLKRPEKLRRAPERRSKDKYYRFHREHDHNTSDC 527
           PYERFTPTTIPISEILTNIEESGMEKLLKRPEKLR APERRSKDKY RFHREH HNTSDC
Sbjct: 284 PYERFTPTTIPISEILTNIEESGMEKLLKRPEKLRGAPERRSKDKYCRFHREHGHNTSDC 343

Query: 528 WELKRQIEDLIQDGYFKKFVGKPRTSSAEKKKERKRSRTPPRRTDRPAVINTIFGGPSGG 587
           WELKRQIEDLIQDGYFKKFVGKPRTSSAEKK+ERKRSRTPPRRTDRPAVINTIFGGPSGG
Sbjct: 344 WELKRQIEDLIQDGYFKKFVGKPRTSSAEKKEERKRSRTPPRRTDRPAVINTIFGGPSGG 403

Query: 588 QSGHKRKELARAARREVCIIREQGPTCPITFNGADLEEVHLPHNDALVIAPLIDHVVVRR 647
           QSGHKRKELARAARREVCIIREQGPTCPITF+GAD EEVHLPHNDA VIAPLIDHVVVRR
Sbjct: 404 QSGHKRKELARAARREVCIIREQGPTCPITFDGADSEEVHLPHNDARVIAPLIDHVVVRR 463

Query: 648 VL 650
           VL
Sbjct: 464 VL 465

BLAST of Moc09g31960 vs. NCBI nr
Match: XP_022152110.1 (uncharacterized protein LOC111019899 [Momordica charantia])

HSP 1 Score: 774.2 bits (1998), Expect = 1.3e-219
Identity = 401/448 (89.51%), Postives = 414/448 (92.41%), Query Frame = 0

Query: 375 MCYFLTGLADEALTVKLGEEAPATFAEVLQKAKKVIDGQELLRTKTGRPERKIGRGRSGK 434
           MCYFLTGLADEALTVKL EEAPATFAEVLQKAKKVIDGQELLRT       KIG+GRSGK
Sbjct: 1   MCYFLTGLADEALTVKLVEEAPATFAEVLQKAKKVIDGQELLRT-------KIGQGRSGK 60

Query: 435 DVERADPKSKDKESFSSGRAEYRRAESGPTRSRPYERFTPTTIPISEILTNIEESGMEKL 494
           D+E  DPKSKDK SFS+GRAEYRRAE+GPTRSRPYERFTPTTIPISEILTNIEESGMEKL
Sbjct: 61  DMENTDPKSKDKGSFSNGRAEYRRAENGPTRSRPYERFTPTTIPISEILTNIEESGMEKL 120

Query: 495 LKRPEKLRRAPERRSKDKYYRFHREHDHNTSDCWELKRQIEDLIQDGYFKKFVGKPRTSS 554
           LKRPEKLR APERRSKDKY RFHREH HNTSD WELK QIEDLIQDGYFKKFVGKPRTSS
Sbjct: 121 LKRPEKLRGAPERRSKDKYCRFHREHGHNTSDYWELKSQIEDLIQDGYFKKFVGKPRTSS 180

Query: 555 AEKKKERKRSRTPPRRTDRPAVINTIFGGPSGGQSGHKRKELARAARREVCIIREQGPTC 614
           AEKK+ERKRSRTPPRRTDRPAVINTIFGGPSGGQSGHKRK+LARAARREVCIIREQ PTC
Sbjct: 181 AEKKEERKRSRTPPRRTDRPAVINTIFGGPSGGQSGHKRKKLARAARREVCIIREQRPTC 240

Query: 615 PITFNGADLEEVHLPHNDALVIAPLIDHVVVRRVLVDGGASANILSLPTYFALGWTRSQL 674
           PITF+ ADL EVHLPHNDALVIAPLIDHVVVRRVLVDGGASANILSLPTY ALGWTRSQL
Sbjct: 241 PITFDXADLXEVHLPHNDALVIAPLIDHVVVRRVLVDGGASANILSLPTYLALGWTRSQL 300

Query: 675 KKSPTPLVGFSGESVIPEGCIDLPVTLGQDRTRVTQMAEFVVIDGRSAYNVIFGRPIIHS 734
           KKSPTPLVGFSGESV+PEGCIDLPVTLGQD+TRVTQMAEFVV+DGRSAYN IFGRPIIHS
Sbjct: 301 KKSPTPLVGFSGESVVPEGCIDLPVTLGQDQTRVTQMAEFVVVDGRSAYNAIFGRPIIHS 360

Query: 735 FRAIPSTLHQVLKYPTLNGVGTVRGEQTASREWYASALKGSSVCALETLPGRDGTLEFEA 794
           FRAIPSTLHQVLKY T NGVGTVRGEQTASRE YAS LKG+SVCALETL  RDGTLEFEA
Sbjct: 361 FRAIPSTLHQVLKYSTPNGVGTVRGEQTASRECYASXLKGTSVCALETLTSRDGTLEFEA 420

Query: 795 NPPRKEFAAPTEELELVSLLSLEKQTDL 823
           + P +EFAAP EELELV LLS EKQ  L
Sbjct: 421 DLPXREFAAPXEELELVPLLSXEKQVQL 441

BLAST of Moc09g31960 vs. ExPASy TrEMBL
Match: A0A6J1C7X5 (uncharacterized protein LOC111008813 OS=Momordica charantia OX=3673 GN=LOC111008813 PE=4 SV=1)

HSP 1 Score: 951.0 bits (2457), Expect = 3.6e-273
Identity = 489/528 (92.61%), Postives = 504/528 (95.45%), Query Frame = 0

Query: 191 QAESSHN---PAGIITREEFDQLRGKLDAQVEALKAKCEQKDDSLNEGDFRESPFTSDVL 250
           +AESS N   PAG+ITREEFDQLRG+LDAQVEALKAKCEQK+  LN+GD  ESPFTSDVL
Sbjct: 3   KAESSRNPATPAGVITREEFDQLRGQLDAQVEALKAKCEQKEGPLNDGDLGESPFTSDVL 62

Query: 251 EAPIPPKFKAPTMKPYDGTKDPKDYVEVFEGLMDFQAASDAIKCRAFQIALTGSARLWYR 310
           EAPIPPKFKAPT+KPYDG+KDPKDYVEVFE LMDFQAASDAIKCRAF+IALTGSARLWYR
Sbjct: 63  EAPIPPKFKAPTVKPYDGSKDPKDYVEVFESLMDFQAASDAIKCRAFRIALTGSARLWYR 122

Query: 311 RLPARSISTYSQLRREFLAQFSSRHYDKKTTTHLATIRQKEGETLREYVTRFQEEQLKVA 370
           RLPA SISTYSQLRREFLA FSSRHYDKKT THLATIRQKEGETLREYVTRFQEEQLKVA
Sbjct: 123 RLPAXSISTYSQLRREFLAXFSSRHYDKKTATHLATIRQKEGETLREYVTRFQEEQLKVA 182

Query: 371 HCSDDSAMCYFLTGLADEALTVKLGEEAPATFAEVLQKAKKVIDGQELLRTKTGRPERKI 430
           HCSDDSAMCYFLTGLADEALTVKLGEEAPATFAEVLQKAKKVIDGQELLRTKTGRPERKI
Sbjct: 183 HCSDDSAMCYFLTGLADEALTVKLGEEAPATFAEVLQKAKKVIDGQELLRTKTGRPERKI 242

Query: 431 GRGRSGKDVERADPKSKDKESFSSGRAEYRRAESGPTRSRPYERFTPTTIPISEILTNIE 490
           GRGRSGKD+E ADPKSKDK SFSSGRAEYRRAE+GPTRSRPYERFTPTTIPISEILTNIE
Sbjct: 243 GRGRSGKDIENADPKSKDKGSFSSGRAEYRRAENGPTRSRPYERFTPTTIPISEILTNIE 302

Query: 491 ESGMEKLLKRPEKLRRAPERRSKDKYYRFHREHDHNTSDCWELKRQIEDLIQDGYFKKFV 550
           ESGMEKLLKRPEKLR APERRSKDKY RFHREH HNTSD WELKRQIE+LIQDGYFKKFV
Sbjct: 303 ESGMEKLLKRPEKLRGAPERRSKDKYCRFHREHGHNTSDYWELKRQIENLIQDGYFKKFV 362

Query: 551 GKPRTSSAEKKKERKRSRTPPRRTDRPAVINTIFGGPSGGQSGHKRKELARAARREVCII 610
           GKPRTSSAEKK+ERKRSRTPPRRTDRPAVINTIFGGPSGGQSG KRKELARAARREVCII
Sbjct: 363 GKPRTSSAEKKEERKRSRTPPRRTDRPAVINTIFGGPSGGQSGRKRKELARAARREVCII 422

Query: 611 REQGPTCPITFNGADLEEVHLPHNDALVIAPLIDHVVVRRVLVDGGASANILSLPTYFAL 670
           REQ PTCPITF+GADLEEVHLPHNDALVIAPLIDHVVV RVLVDGG SANILSLPTY AL
Sbjct: 423 REQRPTCPITFDGADLEEVHLPHNDALVIAPLIDHVVVXRVLVDGGTSANILSLPTYLAL 482

Query: 671 GWTRSQLKKSPTPLVGFSGESVIPEGCIDLPVTLGQDRTRVTQMAEFV 716
           GWTRSQLKKSPTPLVGFSGESVIPEG IDLPVTLGQD+T+VTQMAEFV
Sbjct: 483 GWTRSQLKKSPTPLVGFSGESVIPEGFIDLPVTLGQDQTQVTQMAEFV 530

BLAST of Moc09g31960 vs. ExPASy TrEMBL
Match: A0A6J1D9E1 (uncharacterized protein LOC111018823 OS=Momordica charantia OX=3673 GN=LOC111018823 PE=4 SV=1)

HSP 1 Score: 941.4 bits (2432), Expect = 2.9e-270
Identity = 513/665 (77.14%), Postives = 542/665 (81.50%), Query Frame = 0

Query: 187 SSNQQAESSHNPA---GIITREEFDQLRGKLDAQVEALKAKCEQKDDSLNEGDFRESPFT 246
           SSNQQAESSHNPA   G+ITREEFDQLRGKL+AQVEALKAKCEQK+  LN+GD  ESPFT
Sbjct: 2   SSNQQAESSHNPATPDGVITREEFDQLRGKLNAQVEALKAKCEQKEGPLNDGDLGESPFT 61

Query: 247 SDVLEAPIPPKFKAPTMKPYDGTKDPKDYVEVFEGLMDFQAASDAIKCRAFQIALTGSAR 306
           SDVLE        APT+K YDG+KDPKDYVEVFEGLMDFQAASDAIKCRAFQIALTGSAR
Sbjct: 62  SDVLE--------APTVKSYDGSKDPKDYVEVFEGLMDFQAASDAIKCRAFQIALTGSAR 121

Query: 307 LWYRRLPARSISTYSQLRREFLAQFSSRHYDKKTTTHLATIRQKEGETLREYVTRFQEEQ 366
           LW                                                     FQE+Q
Sbjct: 122 LW-----------------------------------------------------FQEDQ 181

Query: 367 LKVAHCSDDSAMCYFLTGLADEALTVKLGEEAPATFAEVLQKAKKVIDGQELLRTKTGRP 426
           LKVA  SDDSAMCYFLTGLADEALTVKLG+EAPATFAEVLQKAKKVIDGQELLRTKTGRP
Sbjct: 182 LKVAQSSDDSAMCYFLTGLADEALTVKLGKEAPATFAEVLQKAKKVIDGQELLRTKTGRP 241

Query: 427 ERKIGRGRSGKDVERADPKSKDKESFSSGRAEYRRAESGPTRSRPYERFTPTTIPISEIL 486
           ER I RGRSGKD E+AD KSKDK SFSSGRAE+RRA +GPTRSRPYERFTPTTIPISEIL
Sbjct: 242 ERGIDRGRSGKD-EKADLKSKDKGSFSSGRAEFRRAVNGPTRSRPYERFTPTTIPISEIL 301

Query: 487 TNIEESGMEKLLKRPEKLRRAPERRSKDKYYRFHREHDHNTSDCWELKRQIEDLIQDGYF 546
           TNIEESGMEKLLKRPEKLR APERR+KDKY RFHREHDHNTSD WELKRQIEDLIQD YF
Sbjct: 302 TNIEESGMEKLLKRPEKLRGAPERRNKDKYCRFHREHDHNTSDRWELKRQIEDLIQDDYF 361

Query: 547 KKFVGKPRTSSAEKKKERKRSRTPPRRTDRPAVINTIFGGPSGGQSGHKRKELARAARRE 606
           KKFVGKPRTSSAEKK+ERK SRTP RR DRPAVINTIFGGPSGGQSGHKRKELARAARRE
Sbjct: 362 KKFVGKPRTSSAEKKEERKLSRTPLRRIDRPAVINTIFGGPSGGQSGHKRKELARAARRE 421

Query: 607 VCIIREQGPTCPITFNGADLEEVHLPHNDALVIAPLIDHVVVRRVLVDGGASANILSLPT 666
           VCIIREQ PTCPITF+ ADLEEVHLPHNDALVIAPLIDHVVVRRVLVD G SANI+SL T
Sbjct: 422 VCIIREQRPTCPITFDSADLEEVHLPHNDALVIAPLIDHVVVRRVLVDEGVSANIVSLLT 481

Query: 667 YFALGWTRSQLKKSPTPLVGFSGESVIPEGCIDLPVTLGQDRTRVTQMAEFVVIDGRSAY 726
           Y ALGWTRSQLKKS TPLVGFS ESVIPEGCIDLPVTLG D+T+VTQMAEFVVIDGRSAY
Sbjct: 482 YLALGWTRSQLKKSTTPLVGFSRESVIPEGCIDLPVTLGHDQTQVTQMAEFVVIDGRSAY 541

Query: 727 NVIFGRPIIHSFRAIPSTLHQVLKYPTLNGVGTVRGEQTASREWYASALKGSSVCALETL 786
           N IFGRPIIHSFRAIPSTLHQVLKY T NGVG VRGEQ ASRE YASALKGSSVCALETL
Sbjct: 542 NAIFGRPIIHSFRAIPSTLHQVLKYSTPNGVGMVRGEQIASRECYASALKGSSVCALETL 601

Query: 787 PGRDGTLEFEANPPRKEFAAPTEELELVSLLSLEKQTDLARSVPIEILDNPSILEPDLME 846
             RDGTLEF+AN PR+EFAAPTEELELV LL  +   ++     ++   + + ++ D+  
Sbjct: 602 VSRDGTLEFKANLPRREFAAPTEELELVPLLRYKYNENIDHEQELDEKSSLNKIDDDIGV 604

Query: 847 IGAPE 849
            G PE
Sbjct: 662 EGMPE 604

BLAST of Moc09g31960 vs. ExPASy TrEMBL
Match: A0A6J1DHB3 (uncharacterized protein LOC111020479 OS=Momordica charantia OX=3673 GN=LOC111020479 PE=4 SV=1)

HSP 1 Score: 940.6 bits (2430), Expect = 4.9e-270
Identity = 522/790 (66.08%), Postives = 566/790 (71.65%), Query Frame = 0

Query: 1   MVQPANSTNTADRRTLAASDAHQREVGAAAVEGQGHDGLATEPLRRSARITAPALPPAHP 60
           MVQPANSTNTADRR LAA+  HQREVGA  VEGQGH+ L TEPL RSARIT P LPPAHP
Sbjct: 1   MVQPANSTNTADRRALAANHGHQREVGAEVVEGQGHEDLGTEPLCRSARITTPVLPPAHP 60

Query: 61  RTSKATRGRGGTSKKGARGPAPAPTSENFDALQREMEAMRTQMRSMEEMYNEMMLAAGAG 120
           + SK                                                        
Sbjct: 61  KPSK-------------------------------------------------------- 120

Query: 121 SRSENRVTRVDIREQRGSHLGPAEEERPEDNESEGYTRQRGDLREHLNRKRGSSLRKGQS 180
                                                                       
Sbjct: 121 ------------------------------------------------------------ 180

Query: 181 PSRSHRSSNQQAESSHNP--AGIITREEFDQLRGKLDAQVEALKAKCEQKDDSLNEGDFR 240
                      AESS+NP   G+ITREEFDQL+ K DAQVEALKA+CE+K+ S ++GD  
Sbjct: 181 -----------AESSYNPITPGVITREEFDQLKSKFDAQVEALKARCEKKESSFDDGDLG 240

Query: 241 ESPFTSDVLEAPIPPKFKAPTMKPYDGTKDPKDYVEVFEGLMDFQAASDAIKCRAFQIAL 300
           E  F+SD+LEA IPPKFK PTMKPYDG+KDPKDYVEVFE LMDFQAA+DAIKC AFQIAL
Sbjct: 241 ELSFSSDILEALIPPKFKTPTMKPYDGSKDPKDYVEVFESLMDFQAATDAIKCCAFQIAL 300

Query: 301 TGSARLWYRRLPARSISTYSQLRREFLAQFSSRHYDKKTTTHLATIRQKEGETLREYVTR 360
           TGSARLWYRRLPAR ISTYSQLR+EF++QFSSRHYD+KT THLATIRQKEGETLREYVTR
Sbjct: 301 TGSARLWYRRLPARLISTYSQLRKEFISQFSSRHYDRKTPTHLATIRQKEGETLREYVTR 360

Query: 361 FQEEQLKVAHCSDDSAMCYFLTGLADEALTVKLGEEAPATFAEVLQKAKKVIDGQELLRT 420
           F EEQLKVAHCSDDSAMCYFLTGLADE LTVKL EEAPATFAEVLQK KKVIDGQELLRT
Sbjct: 361 FPEEQLKVAHCSDDSAMCYFLTGLADETLTVKLREEAPATFAEVLQKTKKVIDGQELLRT 420

Query: 421 KTGRPERKIGRGRSGKDVERADPKSKDK-ESFSSGRAEYRRAESGPTRSRPYERFTPTTI 480
           KTGRPE+ I +GR+GKD  +AD KS+DK  S SS R +YRR+ S   +SRPYE +TPTTI
Sbjct: 421 KTGRPEKNIDQGRAGKDKGKADSKSRDKGPSSSSSRVDYRRSNSSHNQSRPYEHYTPTTI 480

Query: 481 PISEILTNIEESGMEKLLKRPEKLRRAPERRSKDKYYRFHREHDHNTSDCWELKRQIEDL 540
           PI EILTNIEE+GMEKLLKRPEKLR  PE+R+ DKY RFHR+H HNTS+ WELKRQIEDL
Sbjct: 481 PIFEILTNIEETGMEKLLKRPEKLRGDPEKRNTDKYCRFHRDHGHNTSNYWELKRQIEDL 540

Query: 541 IQDGYFKKFVGKPRTSSAEKKKERKRSRTPPRRTDRPAVINTIFGGPSGGQSGHKRKELA 600
           IQDGYFKKFVGKPR++S EKK+ERKR RTPPRR DRPAVIN             K+KELA
Sbjct: 541 IQDGYFKKFVGKPRSNSVEKKEERKRLRTPPRRDDRPAVIN-------------KKKELA 600

Query: 601 RAARREVCIIREQGPTCPITFNGADLEEVHLPHNDALVIAPLIDHVVVRRVLVDGGASAN 660
           R ARREVCIIREQ PT  I FN ADLE VHLPHNDALVIAPLID V+VRR+LVDGGASAN
Sbjct: 601 REARREVCIIREQRPTSSIAFNHADLEGVHLPHNDALVIAPLIDLVLVRRILVDGGASAN 650

Query: 661 ILSLPTYFALGWTRSQLKKSPTPLVGFSGESVIPEGCIDLPVTLGQDRTRVTQMAEFVVI 720
           ILSL TY ALGWTRSQLKKSPTPLVGFSGES+  EGCIDLPV++ QD T+VTQMAEFVVI
Sbjct: 661 ILSLSTYLALGWTRSQLKKSPTPLVGFSGESISLEGCIDLPVSIRQDDTQVTQMAEFVVI 650

Query: 721 DGRSAYNVIFGRPIIHSFRAIPSTLHQVLKYPTLNGVGTVRGEQTASREWYASALKGSSV 780
           DGRSAYN IFGRPIIHSFRA+PSTLHQVLKY TLNGVGTVRGE   SRE YAS  K SSV
Sbjct: 721 DGRSAYNAIFGRPIIHSFRAVPSTLHQVLKYSTLNGVGTVRGELKTSRECYASVPKRSSV 650

Query: 781 CALETLPGRD 788
           CALE    RD
Sbjct: 781 CALEEQTIRD 650

BLAST of Moc09g31960 vs. ExPASy TrEMBL
Match: A0A6J1D9W7 (uncharacterized protein LOC111018708 OS=Momordica charantia OX=3673 GN=LOC111018708 PE=4 SV=1)

HSP 1 Score: 779.6 bits (2012), Expect = 1.4e-221
Identity = 399/422 (94.55%), Postives = 407/422 (96.45%), Query Frame = 0

Query: 228 KDDSLNEGDFRESPFTSDVLEAPIPPKFKAPTMKPYDGTKDPKDYVEVFEGLMDFQAASD 287
           KDDSLN+GD  ES FTSDVLEAPIPPKFKAPT+KPYDG+KDPKDYVEVFEGLMDF AASD
Sbjct: 44  KDDSLNDGDLGESSFTSDVLEAPIPPKFKAPTVKPYDGSKDPKDYVEVFEGLMDFHAASD 103

Query: 288 AIKCRAFQIALTGSARLWYRRLPARSISTYSQLRREFLAQFSSRHYDKKTTTHLATIRQK 347
           AIKCRAFQIALTGSARLWYRRLPARSISTYSQLRREFLAQFSSR Y KKT THLATIRQK
Sbjct: 104 AIKCRAFQIALTGSARLWYRRLPARSISTYSQLRREFLAQFSSRQYGKKTETHLATIRQK 163

Query: 348 EGETLREYVTRFQEEQLKVAHCSDDSAMCYFLTGLADEALTVKLGEEAPATFAEVLQKAK 407
           EG TLREYVTRFQEEQLKVAHCSDDSAMCYFLTGLADEALTVKLGE+AP TFAEVLQKAK
Sbjct: 164 EGGTLREYVTRFQEEQLKVAHCSDDSAMCYFLTGLADEALTVKLGEKAPTTFAEVLQKAK 223

Query: 408 KVIDGQELLRTKTGRPERKIGRGRSGKDVERADPKSKDKESFSSGRAEYRRAESGPTRSR 467
           KVIDGQELLRTKTGRP+RKIGRGRSGKDVERADPKSKDK SFSSGRAEYRRAESGPT+SR
Sbjct: 224 KVIDGQELLRTKTGRPDRKIGRGRSGKDVERADPKSKDKGSFSSGRAEYRRAESGPTKSR 283

Query: 468 PYERFTPTTIPISEILTNIEESGMEKLLKRPEKLRRAPERRSKDKYYRFHREHDHNTSDC 527
           PYERFTPTTIPISEILTNIEESGMEKLLKRPEKLR APERRSKDKY RFHREH HNTSDC
Sbjct: 284 PYERFTPTTIPISEILTNIEESGMEKLLKRPEKLRGAPERRSKDKYCRFHREHGHNTSDC 343

Query: 528 WELKRQIEDLIQDGYFKKFVGKPRTSSAEKKKERKRSRTPPRRTDRPAVINTIFGGPSGG 587
           WELKRQIEDLIQDGYFKKFVGKPRTSSAEKK+ERKRSRTPPRRTDRPAVINTIFGGPSGG
Sbjct: 344 WELKRQIEDLIQDGYFKKFVGKPRTSSAEKKEERKRSRTPPRRTDRPAVINTIFGGPSGG 403

Query: 588 QSGHKRKELARAARREVCIIREQGPTCPITFNGADLEEVHLPHNDALVIAPLIDHVVVRR 647
           QSGHKRKELARAARREVCIIREQGPTCPITF+GAD EEVHLPHNDA VIAPLIDHVVVRR
Sbjct: 404 QSGHKRKELARAARREVCIIREQGPTCPITFDGADSEEVHLPHNDARVIAPLIDHVVVRR 463

Query: 648 VL 650
           VL
Sbjct: 464 VL 465

BLAST of Moc09g31960 vs. ExPASy TrEMBL
Match: A0A6J1DD03 (uncharacterized protein LOC111019899 OS=Momordica charantia OX=3673 GN=LOC111019899 PE=4 SV=1)

HSP 1 Score: 774.2 bits (1998), Expect = 6.1e-220
Identity = 401/448 (89.51%), Postives = 414/448 (92.41%), Query Frame = 0

Query: 375 MCYFLTGLADEALTVKLGEEAPATFAEVLQKAKKVIDGQELLRTKTGRPERKIGRGRSGK 434
           MCYFLTGLADEALTVKL EEAPATFAEVLQKAKKVIDGQELLRT       KIG+GRSGK
Sbjct: 1   MCYFLTGLADEALTVKLVEEAPATFAEVLQKAKKVIDGQELLRT-------KIGQGRSGK 60

Query: 435 DVERADPKSKDKESFSSGRAEYRRAESGPTRSRPYERFTPTTIPISEILTNIEESGMEKL 494
           D+E  DPKSKDK SFS+GRAEYRRAE+GPTRSRPYERFTPTTIPISEILTNIEESGMEKL
Sbjct: 61  DMENTDPKSKDKGSFSNGRAEYRRAENGPTRSRPYERFTPTTIPISEILTNIEESGMEKL 120

Query: 495 LKRPEKLRRAPERRSKDKYYRFHREHDHNTSDCWELKRQIEDLIQDGYFKKFVGKPRTSS 554
           LKRPEKLR APERRSKDKY RFHREH HNTSD WELK QIEDLIQDGYFKKFVGKPRTSS
Sbjct: 121 LKRPEKLRGAPERRSKDKYCRFHREHGHNTSDYWELKSQIEDLIQDGYFKKFVGKPRTSS 180

Query: 555 AEKKKERKRSRTPPRRTDRPAVINTIFGGPSGGQSGHKRKELARAARREVCIIREQGPTC 614
           AEKK+ERKRSRTPPRRTDRPAVINTIFGGPSGGQSGHKRK+LARAARREVCIIREQ PTC
Sbjct: 181 AEKKEERKRSRTPPRRTDRPAVINTIFGGPSGGQSGHKRKKLARAARREVCIIREQRPTC 240

Query: 615 PITFNGADLEEVHLPHNDALVIAPLIDHVVVRRVLVDGGASANILSLPTYFALGWTRSQL 674
           PITF+ ADL EVHLPHNDALVIAPLIDHVVVRRVLVDGGASANILSLPTY ALGWTRSQL
Sbjct: 241 PITFDXADLXEVHLPHNDALVIAPLIDHVVVRRVLVDGGASANILSLPTYLALGWTRSQL 300

Query: 675 KKSPTPLVGFSGESVIPEGCIDLPVTLGQDRTRVTQMAEFVVIDGRSAYNVIFGRPIIHS 734
           KKSPTPLVGFSGESV+PEGCIDLPVTLGQD+TRVTQMAEFVV+DGRSAYN IFGRPIIHS
Sbjct: 301 KKSPTPLVGFSGESVVPEGCIDLPVTLGQDQTRVTQMAEFVVVDGRSAYNAIFGRPIIHS 360

Query: 735 FRAIPSTLHQVLKYPTLNGVGTVRGEQTASREWYASALKGSSVCALETLPGRDGTLEFEA 794
           FRAIPSTLHQVLKY T NGVGTVRGEQTASRE YAS LKG+SVCALETL  RDGTLEFEA
Sbjct: 361 FRAIPSTLHQVLKYSTPNGVGTVRGEQTASRECYASXLKGTSVCALETLTSRDGTLEFEA 420

Query: 795 NPPRKEFAAPTEELELVSLLSLEKQTDL 823
           + P +EFAAP EELELV LLS EKQ  L
Sbjct: 421 DLPXREFAAPXEELELVPLLSXEKQVQL 441