Moc09g27700 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc09g27700
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Descriptionankyrin repeat-containing protein NPR4-like
Locationchr9: 20731086 .. 20731953 (-)
RNA-Seq ExpressionMoc09g27700
SyntenyMoc09g27700
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCAAACGAAGGATTCTTCGGGTCAACCTCTAACAACTCTCAACAAGCCACTGCCTTTTCAGCCATCAACATGTCATTCAGCCACCCTCTAAGCACAGTTCTCACAGTAAAACTTGACGACAAGAACTACACACTATGGAAAGGAATGGTCCTTGCGGTCTTGCGAGGTCAAAAAGTCGACGGCTATGTTCTCGAGATAAAAACATGTCCTTCCAAATACACTAGGACAACAAGTGACACTGGGTTAGAACCAGCACTCAATCCGGCATACGAAGAATGGTTCGCTGTAGACTAGGCCCTTTGTGGATGGCTGTTCGGGTCCATGACACCATCCATCACTGCTGATGTTGTCAATCTCCAAACCTAAAATGAAGTATGGACTGCTCTAGAAATGCTCTTCGGGTCAACCAGCAAGGCACGCATCAATCAATTAAGAAACAGTCTGCAAAACACCAAAAAGGGAGCCATGAAAATGACAAAATATCTCGCTTTCATGAAACAGACCTCCGAAGATCTGAAACTCGCTGGTGAACCCGTTACGCTAAGCTATTTGTCATCATGCATATTGACGGGCCTTGAGGCAGAATATTTACCGATTCTTTGCACAATCGAAGATAAAGGTATACTATCTTGGCAAGATTTATATTCTACTCTTATAACGTTTGAAAATACTCTTGTGCGACTCAACTTGGTGAATACAAGTGGAGATCTAGGAAATGTCATTGCCAATTATGTTTACAGCCGATAGAGCCAACCCTACAACAATAGACAGCAGCAATATTCTTCAAATCGATTCCATGGACAGTCACGGGAAAGCAACAACAGTGCACGGGGGACTGGTAGAAACAGAGGGCAAGGTGAGGTAG

mRNA sequence

ATGGCAAACGAAGGATTCTTCGGGTCAACCTCTAACAACTCTCAACAAGCCACTGCCTTTTCAGCCATCAACATGTCATTCAGCCACCCTCTAAGCACAGTTCTCACAGTAAAACTTGACGACAAGAACTACACACTATGGAAAGGAATGGTCCTTGCGGTCTTGCGAGGTCAAAAAGTCGACGGCTATGTTCTCGAGATAAAAACATGTCCTTCCAAATACACTAGGACAACAAGTGACACTGGGTTAGAACCAGCACTCAATCCGGCATACGAAGAATGCAAGGCACGCATCAATCAATTAAGAAACAGTCTGCAAAACACCAAAAAGGGAGCCATGAAAATGACAAAATATCTCGCTTTCATGAAACAGACCTCCGAAGATCTGAAACTCGCTGGTGAACCCGTTACGCTAAGCTATTTGTCATCATGCATATTGACGGGCCTTGAGGCAGAATATTTACCGATTCTTTGCACAATCGAAGATAAAGCCGATAGAGCCAACCCTACAACAATAGACAGCAGCAATATTCTTCAAATCGATTCCATGGACAGTCACGGGAAAGCAACAACAGTGCACGGGGGACTGGTAGAAACAGAGGGCAAGGTGAGGTAG

Coding sequence (CDS)

ATGGCAAACGAAGGATTCTTCGGGTCAACCTCTAACAACTCTCAACAAGCCACTGCCTTTTCAGCCATCAACATGTCATTCAGCCACCCTCTAAGCACAGTTCTCACAGTAAAACTTGACGACAAGAACTACACACTATGGAAAGGAATGGTCCTTGCGGTCTTGCGAGGTCAAAAAGTCGACGGCTATGTTCTCGAGATAAAAACATGTCCTTCCAAATACACTAGGACAACAAGTGACACTGGGTTAGAACCAGCACTCAATCCGGCATACGAAGAATGCAAGGCACGCATCAATCAATTAAGAAACAGTCTGCAAAACACCAAAAAGGGAGCCATGAAAATGACAAAATATCTCGCTTTCATGAAACAGACCTCCGAAGATCTGAAACTCGCTGGTGAACCCGTTACGCTAAGCTATTTGTCATCATGCATATTGACGGGCCTTGAGGCAGAATATTTACCGATTCTTTGCACAATCGAAGATAAAGCCGATAGAGCCAACCCTACAACAATAGACAGCAGCAATATTCTTCAAATCGATTCCATGGACAGTCACGGGAAAGCAACAACAGTGCACGGGGGACTGGTAGAAACAGAGGGCAAGGTGAGGTAG

Protein sequence

MANEGFFGSTSNNSQQATAFSAINMSFSHPLSTVLTVKLDDKNYTLWKGMVLAVLRGQKVDGYVLEIKTCPSKYTRTTSDTGLEPALNPAYEECKARINQLRNSLQNTKKGAMKMTKYLAFMKQTSEDLKLAGEPVTLSYLSSCILTGLEAEYLPILCTIEDKADRANPTTIDSSNILQIDSMDSHGKATTVHGGLVETEGKVR
Homology
BLAST of Moc09g27700 vs. NCBI nr
Match: XP_022143579.1 (ankyrin repeat-containing protein NPR4-like [Momordica charantia])

HSP 1 Score: 340.5 bits (872), Expect = 1.0e-89
Identity = 187/248 (75.40%), Postives = 190/248 (76.61%), Query Frame = 0

Query: 1   MANEGFFGSTSNNSQQATAFSAINMSFSHPLSTVLTVKLDDKNYTLWKGMVLAVLRGQKV 60
           MANEGF GST+NNSQQAT FSAIN+SF HPLST LTVKLDDKNYTLWKGMVLAVL GQKV
Sbjct: 1   MANEGFSGSTTNNSQQATVFSAINISFGHPLSTALTVKLDDKNYTLWKGMVLAVLPGQKV 60

Query: 61  DGYVLEIKTCPSKYTRTTSDTG-LEPALNPAYEE-------------------------- 120
           DGYVL  KTCPSKYT TTSDTG LEP LNPAYEE                          
Sbjct: 61  DGYVLRTKTCPSKYTTTTSDTGMLEPTLNPAYEEWSAVDQAFCGWLFGSMTPSIAADVVN 120

Query: 121 ------------------CKARINQLRNSLQNTKKGAMKMTKYLAFMKQTSEDLKLAGEP 180
                              KARINQLRNSLQNTKKGAMKMT YLAFMKQTSEDLKLAGEP
Sbjct: 121 LQTSNEVWTALEMLFGSTSKARINQLRNSLQNTKKGAMKMTXYLAFMKQTSEDLKLAGEP 180

Query: 181 VTLSYLSSCILTGLEAEYLPILCTIEDKADRANPTTIDSSNILQIDSMDSHGKATTVHGG 204
           VTLSYLSSCILTG EAEYLPILCTIEDKADRANPTTID+SNILQIDSMD HGKATTVHGG
Sbjct: 181 VTLSYLSSCILTGXEAEYLPILCTIEDKADRANPTTIDNSNILQIDSMDCHGKATTVHGG 240

BLAST of Moc09g27700 vs. NCBI nr
Match: XP_022157748.1 (uncharacterized protein LOC111024384 isoform X1 [Momordica charantia])

HSP 1 Score: 158.7 bits (400), Expect = 5.4e-35
Identity = 93/186 (50.00%), Postives = 107/186 (57.53%), Query Frame = 0

Query: 24  NMSFSHPLSTVLTVKLDDKNYTLWKGMVLAVLRGQKVDGYVLEIKTCPSKY--TRTTSDT 83
           N SF HPL TVLTVKLDDKNY+LW+GMVLAVLRGQK DGYVL     P ++  +  T  T
Sbjct: 31  NTSFGHPLGTVLTVKLDDKNYSLWRGMVLAVLRGQKFDGYVLGTLAKPPQFLVSPETEGT 90

Query: 84  GLEPALNPAYEE--------------------------------------------CKAR 143
                +NP Y E                                             KAR
Sbjct: 91  SDHLQVNPEYVEWQAVDQALLGWLFGSMTPSIACDVVDFRSSREVWKALEDLYGATSKAR 150

Query: 144 INQLRNSLQNTKKGAMKMTKYLAFMKQTSEDLKLAGEPVTLSYLSSCILTGLEAEYLPIL 164
           INQLRN LQNTKK ++KM++YL  MKQ SE LKLAGEPV  +YL SC+L+GLEAEYLPI+
Sbjct: 151 INQLRNVLQNTKKNSLKMSEYLGLMKQASESLKLAGEPVAFNYLMSCVLSGLEAEYLPIV 210

BLAST of Moc09g27700 vs. NCBI nr
Match: XP_022157750.1 (uncharacterized protein LOC111024384 isoform X2 [Momordica charantia])

HSP 1 Score: 158.7 bits (400), Expect = 5.4e-35
Identity = 93/186 (50.00%), Postives = 107/186 (57.53%), Query Frame = 0

Query: 24  NMSFSHPLSTVLTVKLDDKNYTLWKGMVLAVLRGQKVDGYVLEIKTCPSKY--TRTTSDT 83
           N SF HPL TVLTVKLDDKNY+LW+GMVLAVLRGQK DGYVL     P ++  +  T  T
Sbjct: 31  NTSFGHPLGTVLTVKLDDKNYSLWRGMVLAVLRGQKFDGYVLGTLAKPPQFLVSPETEGT 90

Query: 84  GLEPALNPAYEE--------------------------------------------CKAR 143
                +NP Y E                                             KAR
Sbjct: 91  SDHLQVNPEYVEWQAVDQALLGWLFGSMTPSIACDVVDFRSSREVWKALEDLYGATSKAR 150

Query: 144 INQLRNSLQNTKKGAMKMTKYLAFMKQTSEDLKLAGEPVTLSYLSSCILTGLEAEYLPIL 164
           INQLRN LQNTKK ++KM++YL  MKQ SE LKLAGEPV  +YL SC+L+GLEAEYLPI+
Sbjct: 151 INQLRNVLQNTKKNSLKMSEYLGLMKQASESLKLAGEPVAFNYLMSCVLSGLEAEYLPIV 210

BLAST of Moc09g27700 vs. NCBI nr
Match: GAU21262.1 (hypothetical protein TSUD_286720 [Trifolium subterraneum])

HSP 1 Score: 96.7 bits (239), Expect = 2.5e-16
Identity = 61/187 (32.62%), Postives = 91/187 (48.66%), Query Frame = 0

Query: 21  SAINMSFSHPLSTVLTVKLDDKNYTLWKGMVLAVLRGQKVDGYVLEIKTCPSKYTRTTSD 80
           SA N +  + L T ++VKLD  NY LWK +VL ++RG ++DGY+L  K CP K+  T + 
Sbjct: 3   SAANTNQKNDLPTTVSVKLDRDNYPLWKSLVLPLIRGCRLDGYMLGTKECPEKFVTTNNT 62

Query: 81  TGLEPALNPAYEE--------------------------------------------CKA 140
           T     +NP YEE                                             ++
Sbjct: 63  T---KKVNPEYEEWIAHDQALLGWLRNSMAIDIATQLLHCETSKELWDEAQSLAGAHTRS 122

Query: 141 RINQLRNSLQNTKKGAMKMTKYLAFMKQTSEDLKLAGEPVTLSYLSSCILTGLEAEYLPI 164
           RI  L++   NT+KG MKM +YL  MK  ++ LK+AG P++ S L    L GL+AE+ P+
Sbjct: 123 RIIYLKSEFHNTRKGEMKMDQYLIKMKNLADKLKMAGSPISNSDLMIQTLNGLDAEHNPV 182

BLAST of Moc09g27700 vs. NCBI nr
Match: TXG47047.1 (hypothetical protein EZV62_026341 [Acer yangbiense])

HSP 1 Score: 95.1 bits (235), Expect = 7.3e-16
Identity = 58/168 (34.52%), Postives = 84/168 (50.00%), Query Frame = 0

Query: 29  HPLSTVLTVKLDDKNYTLWKGMVLAVLRGQKVDGYVLEIKTCPSKYTR--TTSDTGLEPA 88
           HPL +  +VKL+ +NY LWK +VL V+RG +++G++   + CP KY     + +   E  
Sbjct: 170 HPLGSYCSVKLNHENYLLWKNLVLPVIRGNRLEGFITGTRKCPPKYVPAIVSEEVSDEAQ 229

Query: 89  LNPAYEE----------------------------CKARINQLRNSLQNTKKGAMKMTKY 148
            NP +EE                             K+ I   +   Q  KKG MKM+ Y
Sbjct: 230 ENPEHEEWVVQDQILLGCETSKDLWDSIKDLFGIKTKSNIAYYKKEFQELKKGGMKMSDY 289

Query: 149 LAFMKQTSEDLKLAGEPVTLSYLSSCILTGLEA-EYLPILCTIEDKAD 166
           L   K+  ++L LAG PV L  L S IL GL++ EY P++C I +K D
Sbjct: 290 LKMAKRLIDNLALAGRPVPLEDLVSQILNGLDSHEYNPLVCQINEKED 337

BLAST of Moc09g27700 vs. ExPASy TrEMBL
Match: A0A6J1CPQ7 (ankyrin repeat-containing protein NPR4-like OS=Momordica charantia OX=3673 GN=LOC111013443 PE=4 SV=1)

HSP 1 Score: 340.5 bits (872), Expect = 4.8e-90
Identity = 187/248 (75.40%), Postives = 190/248 (76.61%), Query Frame = 0

Query: 1   MANEGFFGSTSNNSQQATAFSAINMSFSHPLSTVLTVKLDDKNYTLWKGMVLAVLRGQKV 60
           MANEGF GST+NNSQQAT FSAIN+SF HPLST LTVKLDDKNYTLWKGMVLAVL GQKV
Sbjct: 1   MANEGFSGSTTNNSQQATVFSAINISFGHPLSTALTVKLDDKNYTLWKGMVLAVLPGQKV 60

Query: 61  DGYVLEIKTCPSKYTRTTSDTG-LEPALNPAYEE-------------------------- 120
           DGYVL  KTCPSKYT TTSDTG LEP LNPAYEE                          
Sbjct: 61  DGYVLRTKTCPSKYTTTTSDTGMLEPTLNPAYEEWSAVDQAFCGWLFGSMTPSIAADVVN 120

Query: 121 ------------------CKARINQLRNSLQNTKKGAMKMTKYLAFMKQTSEDLKLAGEP 180
                              KARINQLRNSLQNTKKGAMKMT YLAFMKQTSEDLKLAGEP
Sbjct: 121 LQTSNEVWTALEMLFGSTSKARINQLRNSLQNTKKGAMKMTXYLAFMKQTSEDLKLAGEP 180

Query: 181 VTLSYLSSCILTGLEAEYLPILCTIEDKADRANPTTIDSSNILQIDSMDSHGKATTVHGG 204
           VTLSYLSSCILTG EAEYLPILCTIEDKADRANPTTID+SNILQIDSMD HGKATTVHGG
Sbjct: 181 VTLSYLSSCILTGXEAEYLPILCTIEDKADRANPTTIDNSNILQIDSMDCHGKATTVHGG 240

BLAST of Moc09g27700 vs. ExPASy TrEMBL
Match: A0A6J1DU77 (uncharacterized protein LOC111024384 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111024384 PE=4 SV=1)

HSP 1 Score: 158.7 bits (400), Expect = 2.6e-35
Identity = 93/186 (50.00%), Postives = 107/186 (57.53%), Query Frame = 0

Query: 24  NMSFSHPLSTVLTVKLDDKNYTLWKGMVLAVLRGQKVDGYVLEIKTCPSKY--TRTTSDT 83
           N SF HPL TVLTVKLDDKNY+LW+GMVLAVLRGQK DGYVL     P ++  +  T  T
Sbjct: 31  NTSFGHPLGTVLTVKLDDKNYSLWRGMVLAVLRGQKFDGYVLGTLAKPPQFLVSPETEGT 90

Query: 84  GLEPALNPAYEE--------------------------------------------CKAR 143
                +NP Y E                                             KAR
Sbjct: 91  SDHLQVNPEYVEWQAVDQALLGWLFGSMTPSIACDVVDFRSSREVWKALEDLYGATSKAR 150

Query: 144 INQLRNSLQNTKKGAMKMTKYLAFMKQTSEDLKLAGEPVTLSYLSSCILTGLEAEYLPIL 164
           INQLRN LQNTKK ++KM++YL  MKQ SE LKLAGEPV  +YL SC+L+GLEAEYLPI+
Sbjct: 151 INQLRNVLQNTKKNSLKMSEYLGLMKQASESLKLAGEPVAFNYLMSCVLSGLEAEYLPIV 210

BLAST of Moc09g27700 vs. ExPASy TrEMBL
Match: A0A6J1DTZ7 (uncharacterized protein LOC111024384 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111024384 PE=4 SV=1)

HSP 1 Score: 158.7 bits (400), Expect = 2.6e-35
Identity = 93/186 (50.00%), Postives = 107/186 (57.53%), Query Frame = 0

Query: 24  NMSFSHPLSTVLTVKLDDKNYTLWKGMVLAVLRGQKVDGYVLEIKTCPSKY--TRTTSDT 83
           N SF HPL TVLTVKLDDKNY+LW+GMVLAVLRGQK DGYVL     P ++  +  T  T
Sbjct: 31  NTSFGHPLGTVLTVKLDDKNYSLWRGMVLAVLRGQKFDGYVLGTLAKPPQFLVSPETEGT 90

Query: 84  GLEPALNPAYEE--------------------------------------------CKAR 143
                +NP Y E                                             KAR
Sbjct: 91  SDHLQVNPEYVEWQAVDQALLGWLFGSMTPSIACDVVDFRSSREVWKALEDLYGATSKAR 150

Query: 144 INQLRNSLQNTKKGAMKMTKYLAFMKQTSEDLKLAGEPVTLSYLSSCILTGLEAEYLPIL 164
           INQLRN LQNTKK ++KM++YL  MKQ SE LKLAGEPV  +YL SC+L+GLEAEYLPI+
Sbjct: 151 INQLRNVLQNTKKNSLKMSEYLGLMKQASESLKLAGEPVAFNYLMSCVLSGLEAEYLPIV 210

BLAST of Moc09g27700 vs. ExPASy TrEMBL
Match: A0A2Z6M732 (Integrase catalytic domain-containing protein OS=Trifolium subterraneum OX=3900 GN=TSUD_286720 PE=4 SV=1)

HSP 1 Score: 96.7 bits (239), Expect = 1.2e-16
Identity = 61/187 (32.62%), Postives = 91/187 (48.66%), Query Frame = 0

Query: 21  SAINMSFSHPLSTVLTVKLDDKNYTLWKGMVLAVLRGQKVDGYVLEIKTCPSKYTRTTSD 80
           SA N +  + L T ++VKLD  NY LWK +VL ++RG ++DGY+L  K CP K+  T + 
Sbjct: 3   SAANTNQKNDLPTTVSVKLDRDNYPLWKSLVLPLIRGCRLDGYMLGTKECPEKFVTTNNT 62

Query: 81  TGLEPALNPAYEE--------------------------------------------CKA 140
           T     +NP YEE                                             ++
Sbjct: 63  T---KKVNPEYEEWIAHDQALLGWLRNSMAIDIATQLLHCETSKELWDEAQSLAGAHTRS 122

Query: 141 RINQLRNSLQNTKKGAMKMTKYLAFMKQTSEDLKLAGEPVTLSYLSSCILTGLEAEYLPI 164
           RI  L++   NT+KG MKM +YL  MK  ++ LK+AG P++ S L    L GL+AE+ P+
Sbjct: 123 RIIYLKSEFHNTRKGEMKMDQYLIKMKNLADKLKMAGSPISNSDLMIQTLNGLDAEHNPV 182

BLAST of Moc09g27700 vs. ExPASy TrEMBL
Match: A0A5C7GRG7 (Uncharacterized protein OS=Acer yangbiense OX=1000413 GN=EZV62_026341 PE=4 SV=1)

HSP 1 Score: 95.1 bits (235), Expect = 3.5e-16
Identity = 58/168 (34.52%), Postives = 84/168 (50.00%), Query Frame = 0

Query: 29  HPLSTVLTVKLDDKNYTLWKGMVLAVLRGQKVDGYVLEIKTCPSKYTR--TTSDTGLEPA 88
           HPL +  +VKL+ +NY LWK +VL V+RG +++G++   + CP KY     + +   E  
Sbjct: 170 HPLGSYCSVKLNHENYLLWKNLVLPVIRGNRLEGFITGTRKCPPKYVPAIVSEEVSDEAQ 229

Query: 89  LNPAYEE----------------------------CKARINQLRNSLQNTKKGAMKMTKY 148
            NP +EE                             K+ I   +   Q  KKG MKM+ Y
Sbjct: 230 ENPEHEEWVVQDQILLGCETSKDLWDSIKDLFGIKTKSNIAYYKKEFQELKKGGMKMSDY 289

Query: 149 LAFMKQTSEDLKLAGEPVTLSYLSSCILTGLEA-EYLPILCTIEDKAD 166
           L   K+  ++L LAG PV L  L S IL GL++ EY P++C I +K D
Sbjct: 290 LKMAKRLIDNLALAGRPVPLEDLVSQILNGLDSHEYNPLVCQINEKED 337

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022143579.11.0e-8975.40ankyrin repeat-containing protein NPR4-like [Momordica charantia][more]
XP_022157748.15.4e-3550.00uncharacterized protein LOC111024384 isoform X1 [Momordica charantia][more]
XP_022157750.15.4e-3550.00uncharacterized protein LOC111024384 isoform X2 [Momordica charantia][more]
GAU21262.12.5e-1632.62hypothetical protein TSUD_286720 [Trifolium subterraneum][more]
TXG47047.17.3e-1634.52hypothetical protein EZV62_026341 [Acer yangbiense][more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A6J1CPQ74.8e-9075.40ankyrin repeat-containing protein NPR4-like OS=Momordica charantia OX=3673 GN=LO... [more]
A0A6J1DU772.6e-3550.00uncharacterized protein LOC111024384 isoform X1 OS=Momordica charantia OX=3673 G... [more]
A0A6J1DTZ72.6e-3550.00uncharacterized protein LOC111024384 isoform X2 OS=Momordica charantia OX=3673 G... [more]
A0A2Z6M7321.2e-1632.62Integrase catalytic domain-containing protein OS=Trifolium subterraneum OX=3900 ... [more]
A0A5C7GRG73.5e-1634.52Uncharacterized protein OS=Acer yangbiense OX=1000413 GN=EZV62_026341 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 91..111

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc09g27700.1Moc09g27700.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016020 membrane