Moc09g10060 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc09g10060
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	RNase H domain-containing protein
Location	chr9: 8526732 .. 8527776 (-)
RNA-Seq Expression	Moc09g10060
Synteny	Moc09g10060

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCTTCCAAGTGAGAAATTCTTGATTTGGAGTTAAAATTGAGAGGATGCATCAGCAGATTGTACAAATTCAGAAGGAATCGTAGCGTCTGGATCAATAAATTTTTCTAAACCATGACCTTGAAGAGCAGTAACAACTTGTAGTCGCCATAGCAAGAAGTTCACGTCACTTAATTTTACGGTAGAAATCTTGTTCCCAGGATTAATAACCAGAGATTTCTTATTCTTGTTGACTAAATCAGAGTCACTTTCCGATTCCACCATTGGAGAGAAAAAGGAAAAAAAAAAATCTTGAGTCTTGCAACTTAGGGCTCTGATACCATCATAATTTTCGCAGATAGAAATTTAGATTGTTAAACTGATAATGAGAGAGAAATAGTGTGAGAGAGAGGAAAACTGAATTGATCAATTCTTTCATTTATCCAGAGGATCCAACTAGTATTGTTCAAAGAACAGCGGATGGTTTTGATGAAACATGGAAGTTCGCTGCGCAACTACTAAGAAAGCAGATCTGGCAATGGGCAGATATTAGATGGAACCCTCCTTCGTTTAACTACTGGAAGTTAAACACTGATGGTTCTGCTAAAGGTAATCCAGGGATAGCGGGTGCTGAGGGCATTTTTCGAGATTCTGATGGTAATAGGGTCAAAGGTTACCAACATAACCTTGGCCAAACAGCGGCAGTTATAGCAGAGCTTTGGGGGTTACTATCAGGCCTAGAATTAGCCTGGGAGCTGGGCTGTCGAAAGCTACACGTTGAATTAGACTCTGAAATTGTTTGCAAAATGGTTCATAATCAAAAGCATCCAACTGCTTTGCTAAGGCCTTTTTTAAGGAAGATCACCCAACTGCTAGATCAGGAGTGGGACGTTTCTGTTCATCACATATATAGGGAATCCAATTTTTGTGCAGATTGGTTAGCTAATAATGCTTGGAATCTCCCCATAGGTCTTCACGTGTGTAATGAGCCCCCAACCAAGCTTCTCCATCTGTTGATGGCTGACATCTCAGGGGCCACCATGCCCCGCCTCATCACCTCTTTTTAG

mRNA sequence

ATGCTTCCAAAGGATCCAACTAGTATTGTTCAAAGAACAGCGGATGGTTTTGATGAAACATGGAAGTTCGCTGCGCAACTACTAAGAAAGCAGATCTGGCAATGGGCAGATATTAGATGGAACCCTCCTTCGTTTAACTACTGGAAGTTAAACACTGATGGTTCTGCTAAAGGTAATCCAGGGATAGCGGGTGCTGAGGGCATTTTTCGAGATTCTGATGGTAATAGGGTCAAAGGTTACCAACATAACCTTGGCCAAACAGCGGCAGTTATAGCAGAGCTTTGGGGGTTACTATCAGGCCTAGAATTAGCCTGGGAGCTGGGCTGTCGAAAGCTACACGTTGAATTAGACTCTGAAATTGTTTGCAAAATGGTTCATAATCAAAAGCATCCAACTGCTTTGCTAAGGCCTTTTTTAAGGAAGATCACCCAACTGCTAGATCAGGAGTGGGACGTTTCTGTTCATCACATATATAGGGAATCCAATTTTTGTGCAGATTGGTTAGCTAATAATGCTTGGAATCTCCCCATAGGTCTTCACGTGTGTAATGAGCCCCCAACCAAGCTTCTCCATCTGTTGATGGCTGACATCTCAGGGGCCACCATGCCCCGCCTCATCACCTCTTTTTAG

Coding sequence (CDS)

ATGCTTCCAAAGGATCCAACTAGTATTGTTCAAAGAACAGCGGATGGTTTTGATGAAACATGGAAGTTCGCTGCGCAACTACTAAGAAAGCAGATCTGGCAATGGGCAGATATTAGATGGAACCCTCCTTCGTTTAACTACTGGAAGTTAAACACTGATGGTTCTGCTAAAGGTAATCCAGGGATAGCGGGTGCTGAGGGCATTTTTCGAGATTCTGATGGTAATAGGGTCAAAGGTTACCAACATAACCTTGGCCAAACAGCGGCAGTTATAGCAGAGCTTTGGGGGTTACTATCAGGCCTAGAATTAGCCTGGGAGCTGGGCTGTCGAAAGCTACACGTTGAATTAGACTCTGAAATTGTTTGCAAAATGGTTCATAATCAAAAGCATCCAACTGCTTTGCTAAGGCCTTTTTTAAGGAAGATCACCCAACTGCTAGATCAGGAGTGGGACGTTTCTGTTCATCACATATATAGGGAATCCAATTTTTGTGCAGATTGGTTAGCTAATAATGCTTGGAATCTCCCCATAGGTCTTCACGTGTGTAATGAGCCCCCAACCAAGCTTCTCCATCTGTTGATGGCTGACATCTCAGGGGCCACCATGCCCCGCCTCATCACCTCTTTTTAG

Protein sequence

MLPKDPTSIVQRTADGFDETWKFAAQLLRKQIWQWADIRWNPPSFNYWKLNTDGSAKGNPGIAGAEGIFRDSDGNRVKGYQHNLGQTAAVIAELWGLLSGLELAWELGCRKLHVELDSEIVCKMVHNQKHPTALLRPFLRKITQLLDQEWDVSVHHIYRESNFCADWLANNAWNLPIGLHVCNEPPTKLLHLLMADISGATMPRLITSF

Homology

[Close]

BLAST of Moc09g10060 vs. NCBI nr
Match: XP_031402735.1 (uncharacterized protein LOC116212324 [Punica granatum])

HSP 1 Score: 181.0 bits (458), Expect = 1.0e-41
Identity = 88/203 (43.35%), Postives = 127/203 (62.56%), Query Frame = 0


Query: 3    PKDPTSIVQRTADGFDETWKFAAQLLRKQIWQWADIRWNPPSFNYWKLNTDGSAKGNPGI 62
            P+D    + R A+ F   W+    ++R+ + +W  + WN PS  + KLNTDG++KGNPG 
Sbjct: 1007 PQDAHVSILRAAESFSVGWQDQV-VVRRTVREWQLVGWNRPSRGWIKLNTDGASKGNPGP 1066

Query: 63   AGAEGIFRDSDGNRVKGYQHNLGQTAAVIAELWGLLSGLELAWELGCRKLHVELDSEIVC 122
            AGA G+ R  DG     +  N+G   AV+AELWG+LSGLELAWELG R + +E+DS +V 
Sbjct: 1067 AGAGGVLRKEDGVWTASFARNVGIATAVVAELWGVLSGLELAWELGYRLVLLEVDSLLVT 1126

Query: 123  KMVHNQKHPTALLRPFLRKITQLLDQEWDVSVHHIYRESNFCADWLANNAWNLPIGLHVC 182
            +++         LR  +R+I   L+++W V V H YRE N  ADW+A  + +LP+GLH+ 
Sbjct: 1127 RLIAGSGPRAPQLRTIVREIRSWLERDWQVEVSHQYREGNSVADWMARWSLSLPLGLHIQ 1186

Query: 183  NEPPTKLLHLLMADISGATMPRL 206
            + PP ++  LL  DI GA +PRL
Sbjct: 1187 HIPPPEVRALLAGDIIGAALPRL 1208

[Close]

BLAST of Moc09g10060 vs. NCBI nr
Match: PKI42247.1 (hypothetical protein CRG98_037363 [Punica granatum])

HSP 1 Score: 178.7 bits (452), Expect = 5.2e-41
Identity = 80/205 (39.02%), Postives = 122/205 (59.51%), Query Frame = 0


Query: 3   PKDPTSIVQRTADGFDETWKFAAQLLRKQIWQWADIRWNPPSFNYWKLNTDGSAKGNPGI 62
           P D    +   A  F E W  ++Q++     +W  I W  P  ++ KLNTDG  +G+ G+
Sbjct: 66  PSDEGHAILAVARSFQEGWLLSSQVVDGSKRKWISIHWVKPLEDFMKLNTDGDVRGSQGV 125

Query: 63  AGAEGIFRDSDGNRVKGYQHNLGQTAAVIAELWGLLSGLELAWELGCRKLHVELDSEIVC 122
            GA GI R+S G  + G+  +LG +   +AELWG+L+GLELAW   CR+L +E+DS +V 
Sbjct: 126 TGAGGILRNSSGGWIIGFMQSLGVSTVTVAELWGVLTGLELAWNARCRRLIIEMDSMVVL 185

Query: 123 KMVHNQKHPTALLRPFLRKITQLLDQEWDVSVHHIYRESNFCADWLANNAWNLPIGLHVC 182
            +V  Q   +   +P  R I  LL ++W+V + H Y E N CADWL+  A +LP+G +V 
Sbjct: 186 TLVLRQDSCSPCFQPLQRSIRNLLQRDWEVHIQHTYWEGNPCADWLSKRAIDLPLGTYVL 245

Query: 183 NEPPTKLLHLLMADISGATMPRLIT 208
           +  P ++L LL+ DI+GA + R ++
Sbjct: 246 HSAPNEMLSLLLGDIAGAVLSRFVS 270

[Close]

BLAST of Moc09g10060 vs. NCBI nr
Match: XP_031376896.1 (uncharacterized protein LOC116192475 [Punica granatum])

HSP 1 Score: 168.7 bits (426), Expect = 5.3e-38
Identity = 79/203 (38.92%), Postives = 124/203 (61.08%), Query Frame = 0


Query: 3   PKDPTSIVQRTADGFDETWKFAAQLLR-KQIWQWADIRWNPPSFNYWKLNTDGSAKGNPG 62
           P+D    ++ TA  F+      +   + ++ W+W  I+W  P   + K+NTDG+++GNPG
Sbjct: 209 PRDEVRAIRATAHNFEHARDLRSNTSQPRREWRW--IQWRRPPPGWVKVNTDGASRGNPG 268

Query: 63  IAGAEGIFRDSDGNRVKGYQHNLGQTAAVIAELWGLLSGLELAWELGCRKLHVELDSEIV 122
           IAGA G+ R+ DG+ + G+ HN+G   + IAELWG L GL+LAW+ G R + +ELDS++V
Sbjct: 269 IAGAGGLLRNDDGHWICGFLHNVGFATSTIAELWGALVGLQLAWDRGYRHVVLELDSQVV 328

Query: 123 CKMVHNQKHPTALLRPFLRKITQLLDQEWDVSVHHIYRESNFCADWLANNAWNLPIGLHV 182
            +++ +       L P +  I+ +L ++W + V H YRE N CADW+A  A +  +G H 
Sbjct: 329 QRLLSDGSTHNQCLEPLVSSISDILHRDWHLEVSHTYREGNACADWMARWAVSFSLGSHF 388

Query: 183 CNEPPTKLLHLLMADISGATMPR 205
              PP  L  LL  D++GA++PR
Sbjct: 389 LVAPPIGLHALLEGDLAGASLPR 409

[Close]

BLAST of Moc09g10060 vs. NCBI nr
Match: PKI65311.1 (hypothetical protein CRG98_014275 [Punica granatum])

HSP 1 Score: 155.6 bits (392), Expect = 4.7e-34
Identity = 76/177 (42.94%), Postives = 108/177 (61.02%), Query Frame = 0


Query: 29  RKQIWQWADIRWNPPSFNYWKLNTDGSAKGNPGIAGAEGIFRDSDGNRVKGYQHNLGQTA 88
           RK + +W  + W  PS  + KLNTDG++KGNP  AGA G+ R  DG  + G+   +G   
Sbjct: 10  RKTVKEWQLMGWKQPSPGWIKLNTDGASKGNPSPAGAGGVLRKEDGVWIVGFARYVGIAT 69

Query: 89  AVIAELWGLLSGLELAWELGCRKLHVELDSEIVCKMVHNQKHPTALLRPFLRKITQLLDQ 148
           A +AELWG+LSGLELAWELG R + +E+DS +V +++         LR  +R I   L++
Sbjct: 70  AAVAELWGVLSGLELAWELGYRHVLLEVDSLLVTRLISGSGSRAPQLRTIVRAIHSWLER 129

Query: 149 EWDVSVHHIYRESNFCADWLANNAWNLPIGLHVCNEPPTKLLHLLMADISGATMPRL 206
           +W V V H YRE N  ADW+A  + +L +GLH+ + P  ++  LL  DI    +PRL
Sbjct: 130 DWQVEVSHQYREGNSVADWMARWSLSLALGLHIRHTPLPEVRALLSGDIVEVVLPRL 186

[Close]

BLAST of Moc09g10060 vs. NCBI nr
Match: PKI39106.1 (hypothetical protein CRG98_040530 [Punica granatum])

HSP 1 Score: 154.5 bits (389), Expect = 1.0e-33
Identity = 78/198 (39.39%), Postives = 119/198 (60.10%), Query Frame = 0


Query: 10  VQRTADGFD--ETWKFAAQLLRKQIWQWADIRWNPPSFNYWKLNTDGSAKGNPGIAGAEG 69
           +Q  A GF+   T + +    RK+ W+W  +RW  P   + K+NTDG+++GNPG+AGA G
Sbjct: 339 IQVVASGFECANTSRTSCVKPRKE-WRW--VRWRRPPPGWIKVNTDGASRGNPGLAGAGG 398

Query: 70  IFRDSDGNRVKGYQHNLGQTAAVIAELWGLLSGLELAWELGCRKLHVELDSEIVCKMVHN 129
           +    DG  + G+ HN+G   + IA+LWG L GLELAW+ G R + +ELDS++V +++ N
Sbjct: 399 LICGDDGRWICGFVHNVGFATSTIAKLWGALVGLELAWDRGYRYVVLELDSQVVQRLLSN 458

Query: 130 QKHPTALLRPFLRKITQLLDQEWDVSVHHIYRESNFCADWLANNAWNLPIGLHVCNEPPT 189
           Q    + L P +  I  +L ++W V V H Y+ESN CADW+A  A +  +G +     P 
Sbjct: 459 QPPTNSYLDPLVNNINNILYRDWHVEVSHTYQESNMCADWMARWATSFSLGSYFFVATPI 518

Query: 190 KLLHLLMADISGATMPRL 206
            +  LL  +  GA++ RL
Sbjct: 519 GINALLEGNFVGASISRL 533

[Close]

BLAST of Moc09g10060 vs. ExPASy Swiss-Prot
Match: P0C2F6 (Putative ribonuclease H protein At1g65750 OS=Arabidopsis thaliana OX=3702 GN=At1g65750 PE=3 SV=1)

HSP 1 Score: 125.2 bits (313), Expect = 8.9e-28
Identity = 66/172 (38.37%), Postives = 99/172 (57.56%), Query Frame = 0


Query: 38  IRWNPPSFNYWKLNTDGSAKGNPGIAGAEGIFRDSDGNRVKGYQHNLGQTAAVIAELWGL 97
           I W  P   + K+NTDG+++GNPG+A A G+ RD  G    G+  N+G+ +A  AELWG+
Sbjct: 450 IGWVSPCVGWVKVNTDGASRGNPGLASAGGVLRDCTGAWCGGFSLNIGRCSAPQAELWGV 509

Query: 98  LSGLELAWELGCRKLHVELDSEIV---CKMVHNQKHPTALLRPFLRKITQLLDQEWDVSV 157
             GL  AWE    ++ +E+DSE++    K   +  HP + L   +R     L ++W V +
Sbjct: 510 YYGLYFAWEKKVPRVELEVDSEVIVGFLKTGISDSHPLSFL---VRLCHGFLQKDWLVRI 569

Query: 158 HHIYRESNFCADWLANNAWNLPIGLHVCNEPPTKLLHLLMADISGATMPRLI 207
            H+YRE+N  AD LAN A++L +G H  +  P  +  LL  D  G+T PR +
Sbjct: 570 VHVYREANRLADGLANYAFSLSLGFHSFDLVPDAMSSLLREDTLGSTRPRRV 618

[Close]

BLAST of Moc09g10060 vs. ExPASy TrEMBL
Match: A0A6P8E5K3 (uncharacterized protein LOC116212324 OS=Punica granatum OX=22663 GN=LOC116212324 PE=4 SV=1)

HSP 1 Score: 181.0 bits (458), Expect = 5.0e-42
Identity = 88/203 (43.35%), Postives = 127/203 (62.56%), Query Frame = 0


Query: 3    PKDPTSIVQRTADGFDETWKFAAQLLRKQIWQWADIRWNPPSFNYWKLNTDGSAKGNPGI 62
            P+D    + R A+ F   W+    ++R+ + +W  + WN PS  + KLNTDG++KGNPG 
Sbjct: 1007 PQDAHVSILRAAESFSVGWQDQV-VVRRTVREWQLVGWNRPSRGWIKLNTDGASKGNPGP 1066

Query: 63   AGAEGIFRDSDGNRVKGYQHNLGQTAAVIAELWGLLSGLELAWELGCRKLHVELDSEIVC 122
            AGA G+ R  DG     +  N+G   AV+AELWG+LSGLELAWELG R + +E+DS +V 
Sbjct: 1067 AGAGGVLRKEDGVWTASFARNVGIATAVVAELWGVLSGLELAWELGYRLVLLEVDSLLVT 1126

Query: 123  KMVHNQKHPTALLRPFLRKITQLLDQEWDVSVHHIYRESNFCADWLANNAWNLPIGLHVC 182
            +++         LR  +R+I   L+++W V V H YRE N  ADW+A  + +LP+GLH+ 
Sbjct: 1127 RLIAGSGPRAPQLRTIVREIRSWLERDWQVEVSHQYREGNSVADWMARWSLSLPLGLHIQ 1186

Query: 183  NEPPTKLLHLLMADISGATMPRL 206
            + PP ++  LL  DI GA +PRL
Sbjct: 1187 HIPPPEVRALLAGDIIGAALPRL 1208

[Close]

BLAST of Moc09g10060 vs. ExPASy TrEMBL
Match: A0A2I0IE25 (RNase H domain-containing protein OS=Punica granatum OX=22663 GN=CRG98_037363 PE=4 SV=1)

HSP 1 Score: 178.7 bits (452), Expect = 2.5e-41
Identity = 80/205 (39.02%), Postives = 122/205 (59.51%), Query Frame = 0


Query: 3   PKDPTSIVQRTADGFDETWKFAAQLLRKQIWQWADIRWNPPSFNYWKLNTDGSAKGNPGI 62
           P D    +   A  F E W  ++Q++     +W  I W  P  ++ KLNTDG  +G+ G+
Sbjct: 66  PSDEGHAILAVARSFQEGWLLSSQVVDGSKRKWISIHWVKPLEDFMKLNTDGDVRGSQGV 125

Query: 63  AGAEGIFRDSDGNRVKGYQHNLGQTAAVIAELWGLLSGLELAWELGCRKLHVELDSEIVC 122
            GA GI R+S G  + G+  +LG +   +AELWG+L+GLELAW   CR+L +E+DS +V 
Sbjct: 126 TGAGGILRNSSGGWIIGFMQSLGVSTVTVAELWGVLTGLELAWNARCRRLIIEMDSMVVL 185

Query: 123 KMVHNQKHPTALLRPFLRKITQLLDQEWDVSVHHIYRESNFCADWLANNAWNLPIGLHVC 182
            +V  Q   +   +P  R I  LL ++W+V + H Y E N CADWL+  A +LP+G +V 
Sbjct: 186 TLVLRQDSCSPCFQPLQRSIRNLLQRDWEVHIQHTYWEGNPCADWLSKRAIDLPLGTYVL 245

Query: 183 NEPPTKLLHLLMADISGATMPRLIT 208
           +  P ++L LL+ DI+GA + R ++
Sbjct: 246 HSAPNEMLSLLLGDIAGAVLSRFVS 270

[Close]

BLAST of Moc09g10060 vs. ExPASy TrEMBL
Match: A0A6P8C599 (uncharacterized protein LOC116192475 OS=Punica granatum OX=22663 GN=LOC116192475 PE=4 SV=1)

HSP 1 Score: 168.7 bits (426), Expect = 2.6e-38
Identity = 79/203 (38.92%), Postives = 124/203 (61.08%), Query Frame = 0


Query: 3   PKDPTSIVQRTADGFDETWKFAAQLLR-KQIWQWADIRWNPPSFNYWKLNTDGSAKGNPG 62
           P+D    ++ TA  F+      +   + ++ W+W  I+W  P   + K+NTDG+++GNPG
Sbjct: 209 PRDEVRAIRATAHNFEHARDLRSNTSQPRREWRW--IQWRRPPPGWVKVNTDGASRGNPG 268

Query: 63  IAGAEGIFRDSDGNRVKGYQHNLGQTAAVIAELWGLLSGLELAWELGCRKLHVELDSEIV 122
           IAGA G+ R+ DG+ + G+ HN+G   + IAELWG L GL+LAW+ G R + +ELDS++V
Sbjct: 269 IAGAGGLLRNDDGHWICGFLHNVGFATSTIAELWGALVGLQLAWDRGYRHVVLELDSQVV 328

Query: 123 CKMVHNQKHPTALLRPFLRKITQLLDQEWDVSVHHIYRESNFCADWLANNAWNLPIGLHV 182
            +++ +       L P +  I+ +L ++W + V H YRE N CADW+A  A +  +G H 
Sbjct: 329 QRLLSDGSTHNQCLEPLVSSISDILHRDWHLEVSHTYREGNACADWMARWAVSFSLGSHF 388

Query: 183 CNEPPTKLLHLLMADISGATMPR 205
              PP  L  LL  D++GA++PR
Sbjct: 389 LVAPPIGLHALLEGDLAGASLPR 409

[Close]

BLAST of Moc09g10060 vs. ExPASy TrEMBL
Match: A0A2I0K9U3 (RNase H domain-containing protein OS=Punica granatum OX=22663 GN=CRG98_014275 PE=4 SV=1)

HSP 1 Score: 155.6 bits (392), Expect = 2.3e-34
Identity = 76/177 (42.94%), Postives = 108/177 (61.02%), Query Frame = 0


Query: 29  RKQIWQWADIRWNPPSFNYWKLNTDGSAKGNPGIAGAEGIFRDSDGNRVKGYQHNLGQTA 88
           RK + +W  + W  PS  + KLNTDG++KGNP  AGA G+ R  DG  + G+   +G   
Sbjct: 10  RKTVKEWQLMGWKQPSPGWIKLNTDGASKGNPSPAGAGGVLRKEDGVWIVGFARYVGIAT 69

Query: 89  AVIAELWGLLSGLELAWELGCRKLHVELDSEIVCKMVHNQKHPTALLRPFLRKITQLLDQ 148
           A +AELWG+LSGLELAWELG R + +E+DS +V +++         LR  +R I   L++
Sbjct: 70  AAVAELWGVLSGLELAWELGYRHVLLEVDSLLVTRLISGSGSRAPQLRTIVRAIHSWLER 129

Query: 149 EWDVSVHHIYRESNFCADWLANNAWNLPIGLHVCNEPPTKLLHLLMADISGATMPRL 206
           +W V V H YRE N  ADW+A  + +L +GLH+ + P  ++  LL  DI    +PRL
Sbjct: 130 DWQVEVSHQYREGNSVADWMARWSLSLALGLHIRHTPLPEVRALLSGDIVEVVLPRL 186

[Close]

BLAST of Moc09g10060 vs. ExPASy TrEMBL
Match: A0A2I0I6R1 (RNase H domain-containing protein OS=Punica granatum OX=22663 GN=CRG98_040530 PE=4 SV=1)

HSP 1 Score: 154.5 bits (389), Expect = 5.0e-34
Identity = 78/198 (39.39%), Postives = 119/198 (60.10%), Query Frame = 0


Query: 10  VQRTADGFD--ETWKFAAQLLRKQIWQWADIRWNPPSFNYWKLNTDGSAKGNPGIAGAEG 69
           +Q  A GF+   T + +    RK+ W+W  +RW  P   + K+NTDG+++GNPG+AGA G
Sbjct: 339 IQVVASGFECANTSRTSCVKPRKE-WRW--VRWRRPPPGWIKVNTDGASRGNPGLAGAGG 398

Query: 70  IFRDSDGNRVKGYQHNLGQTAAVIAELWGLLSGLELAWELGCRKLHVELDSEIVCKMVHN 129
           +    DG  + G+ HN+G   + IA+LWG L GLELAW+ G R + +ELDS++V +++ N
Sbjct: 399 LICGDDGRWICGFVHNVGFATSTIAKLWGALVGLELAWDRGYRYVVLELDSQVVQRLLSN 458

Query: 130 QKHPTALLRPFLRKITQLLDQEWDVSVHHIYRESNFCADWLANNAWNLPIGLHVCNEPPT 189
           Q    + L P +  I  +L ++W V V H Y+ESN CADW+A  A +  +G +     P 
Sbjct: 459 QPPTNSYLDPLVNNINNILYRDWHVEVSHTYQESNMCADWMARWATSFSLGSYFFVATPI 518

Query: 190 KLLHLLMADISGATMPRL 206
            +  LL  +  GA++ RL
Sbjct: 519 GINALLEGNFVGASISRL 533

[Close]

BLAST of Moc09g10060 vs. TAIR 10
Match: AT5G42905.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 83.6 bits (205), Expect = 2.1e-16
Identity = 43/118 (36.44%), Postives = 67/118 (56.78%), Query Frame = 0


Query: 38  IRWNPPSFNYWKLNTDGSAKGNPGIAGAEGIFRDSDGNRVKGYQHNLGQTAAVIAELWGL 97
           I W  P   + K+NTDG+++GNPG+A A G+ RD +G    G+  N+G+ +A  AELWG+
Sbjct: 138 IGWVLPCVGWVKVNTDGASRGNPGLASAGGVLRDCEGAWCGGFSLNIGRCSAQHAELWGV 197

Query: 98  LSGLELAWELGCRKLHVELDSEIV---CKMVHNQKHPTALLRPFLRKITQLLDQEWDV 153
             GL  AWE    ++ +E+DSE +    K   +  HP + L   +R     L ++W +
Sbjct: 198 YYGLYFAWEKKVPRVELEVDSEAIVGFLKTGISDSHPLSFL---VRLCHNFLQKDWRI 252

[Close]

BLAST of Moc09g10060 vs. TAIR 10
Match: AT5G61090.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 79.7 bits (195), Expect = 3.0e-15
Identity = 45/157 (28.66%), Postives = 70/157 (44.59%), Query Frame = 0


Query: 40  WNPPSFNYWKLNTDGSAKGNPGIAGAEGIFRDSDGNRVKGYQHNLGQTAAVIAELWGLLS 99
           W PP     KLN  G++     +  + GI RD  G  V GY         V+A L  +  
Sbjct: 499 WTPPLV---KLNIQGTSNPLSDLTRSAGIVRDQSGKWVFGYIRCHKSIPEVVAGLLAIYQ 558

Query: 100 GLELAWELGCRKLHVELDSEIVCKMVHNQKHPTALLRPFLRKITQLLDQEWDVSVHHIYR 159
           GL+  W+ G R++H+E  S  +   +  +       +  L     ++ +EW+  ++HI +
Sbjct: 559 GLKYLWDSGFRRIHLETTSFEIINALTTKSSLFCKSKTLLGACKDMILKEWECDIYHISK 618

Query: 160 ESNFCADWLANNAWNLPIGLHVCNEPPTKLLHLLMAD 197
           E N CA+WLA  +   P  L     PP  L+  L  D
Sbjct: 619 EQNSCAEWLAKRSEEQPQELVFFEYPPRGLMDFLEKD 652

[Close]

BLAST of Moc09g10060 vs. TAIR 10
Match: AT5G51080.1 (RNase H family protein )

HSP 1 Score: 72.4 bits (176), Expect = 4.8e-13
Identity = 44/127 (34.65%), Postives = 66/127 (51.97%), Query Frame = 0


Query: 53  DGSAKGNPGIAGAEGIFRDSDGNRVKGYQHNLGQTAAVIAELWGLLSGLELAWELGCRKL 112
           DG++KGNPG++GA  + +  DG+ +   +  LG      AE  GL+ GL+ A E G  K+
Sbjct: 191 DGASKGNPGLSGAAAVLKTEDGSLIFKMRQGLGIATNNAAEYHGLILGLKHAIEKGYTKI 250

Query: 113 HVELDSEIVCKMVHNQ-KHPTALLRPFLRKITQLLDQEWDVSVHHIYRESNFCADWLANN 172
            V+ DS++VC  +  Q K    +L    ++  QL D+     + H+ R  N  AD  AN 
Sbjct: 251 KVKTDSKLVCMQMKGQWKVNHEVLSKLHKEAKQLSDKCLSFEISHVLRSLNSDADEQANM 310

Query: 173 AWNLPIG 179
           A  L  G
Sbjct: 311 AARLSEG 317

[Close]

BLAST of Moc09g10060 vs. TAIR 10
Match: AT5G51080.2 (RNase H family protein )

HSP 1 Score: 72.4 bits (176), Expect = 4.8e-13
Identity = 44/127 (34.65%), Postives = 66/127 (51.97%), Query Frame = 0


Query: 53  DGSAKGNPGIAGAEGIFRDSDGNRVKGYQHNLGQTAAVIAELWGLLSGLELAWELGCRKL 112
           DG++KGNPG++GA  + +  DG+ +   +  LG      AE  GL+ GL+ A E G  K+
Sbjct: 191 DGASKGNPGLSGAAAVLKTEDGSLIFKMRQGLGIATNNAAEYHGLILGLKHAIEKGYTKI 250

Query: 113 HVELDSEIVCKMVHNQ-KHPTALLRPFLRKITQLLDQEWDVSVHHIYRESNFCADWLANN 172
            V+ DS++VC  +  Q K    +L    ++  QL D+     + H+ R  N  AD  AN 
Sbjct: 251 KVKTDSKLVCMQMKGQWKVNHEVLSKLHKEAKQLSDKCLSFEISHVLRSLNSDADEQANM 310

Query: 173 AWNLPIG 179
           A  L  G
Sbjct: 311 AARLSEG 317

[Close]

BLAST of Moc09g10060 vs. TAIR 10
Match: AT5G51080.3 (RNase H family protein )

HSP 1 Score: 72.4 bits (176), Expect = 4.8e-13
Identity = 44/127 (34.65%), Postives = 66/127 (51.97%), Query Frame = 0


Query: 53  DGSAKGNPGIAGAEGIFRDSDGNRVKGYQHNLGQTAAVIAELWGLLSGLELAWELGCRKL 112
           DG++KGNPG++GA  + +  DG+ +   +  LG      AE  GL+ GL+ A E G  K+
Sbjct: 128 DGASKGNPGLSGAAAVLKTEDGSLIFKMRQGLGIATNNAAEYHGLILGLKHAIEKGYTKI 187

Query: 113 HVELDSEIVCKMVHNQ-KHPTALLRPFLRKITQLLDQEWDVSVHHIYRESNFCADWLANN 172
            V+ DS++VC  +  Q K    +L    ++  QL D+     + H+ R  N  AD  AN 
Sbjct: 188 KVKTDSKLVCMQMKGQWKVNHEVLSKLHKEAKQLSDKCLSFEISHVLRSLNSDADEQANM 247

Query: 173 AWNLPIG 179
           A  L  G
Sbjct: 248 AARLSEG 254

The following BLAST results are available for this feature:

BLAST of Moc09g10060 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_031402735.1	1.0e-41	43.35	uncharacterized protein LOC116212324 [Punica granatum]	[more]
PKI42247.1	5.2e-41	39.02	hypothetical protein CRG98_037363 [Punica granatum]	[more]
XP_031376896.1	5.3e-38	38.92	uncharacterized protein LOC116192475 [Punica granatum]	[more]
PKI65311.1	4.7e-34	42.94	hypothetical protein CRG98_014275 [Punica granatum]	[more]
PKI39106.1	1.0e-33	39.39	hypothetical protein CRG98_040530 [Punica granatum]	[more]

BLAST of Moc09g10060 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P0C2F6	8.9e-28	38.37	Putative ribonuclease H protein At1g65750 OS=Arabidopsis thaliana OX=3702 GN=At1...	[more]

BLAST of Moc09g10060 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6P8E5K3	5.0e-42	43.35	uncharacterized protein LOC116212324 OS=Punica granatum OX=22663 GN=LOC116212324...	[more]
A0A2I0IE25	2.5e-41	39.02	RNase H domain-containing protein OS=Punica granatum OX=22663 GN=CRG98_037363 PE...	[more]
A0A6P8C599	2.6e-38	38.92	uncharacterized protein LOC116192475 OS=Punica granatum OX=22663 GN=LOC116192475...	[more]
A0A2I0K9U3	2.3e-34	42.94	RNase H domain-containing protein OS=Punica granatum OX=22663 GN=CRG98_014275 PE...	[more]
A0A2I0I6R1	5.0e-34	39.39	RNase H domain-containing protein OS=Punica granatum OX=22663 GN=CRG98_040530 PE...	[more]

BLAST of Moc09g10060 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G42905.1	2.1e-16	36.44	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]
AT5G61090.1	3.0e-15	28.66	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]
AT5G51080.1	4.8e-13	34.65	RNase H family protein	[more]
AT5G51080.2	4.8e-13	34.65	RNase H family protein	[more]
AT5G51080.3	4.8e-13	34.65	RNase H family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002156	Ribonuclease H domain	PFAM	PF13456	RVT_3	coord: 51..172 e-value: 1.2E-30 score: 105.9
IPR002156	Ribonuclease H domain	PROSITE	PS50879	RNASE_H	coord: 44..174 score: 12.499501
IPR036397	Ribonuclease H superfamily	GENE3D	3.30.420.10		coord: 46..174 e-value: 2.1E-27 score: 97.8
None	No IPR available	PANTHER	PTHR47723	OS05G0353850 PROTEIN	coord: 30..198
IPR044730	Ribonuclease H-like domain, plant type	CDD	cd06222	RNase_H_like	coord: 50..170 e-value: 5.30657E-36 score: 120.882
IPR012337	Ribonuclease H-like superfamily	SUPERFAMILY	53098	Ribonuclease H-like	coord: 46..170

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc09g10060.1	Moc09g10060.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0004523	RNA-DNA hybrid ribonuclease activity