Moc09g07920 (gene) Bitter gourd (OHB3-1) v2

BLAST of Moc09g07920 vs. NCBI nr
Match: XP_022150231.1 (isoamylase 2, chloroplastic [Momordica charantia])

HSP 1 Score: 1814.3 bits (4698), Expect = 0.0e+00
Identity = 891/891 (100.00%), Postives = 891/891 (100.00%), Query Frame = 0

Query: 1   MLLQPQMATFPSSLVVQPYNVHNFGVTEAFRLAASDHHTHGHMIKHQSGKRDEVRMLAHG 60
           MLLQPQMATFPSSLVVQPYNVHNFGVTEAFRLAASDHHTHGHMIKHQSGKRDEVRMLAHG
Sbjct: 1   MLLQPQMATFPSSLVVQPYNVHNFGVTEAFRLAASDHHTHGHMIKHQSGKRDEVRMLAHG 60

Query: 61  ESRAGFAKSSRYNLSKAYASFGISVEKNGWRFGISGKSKEQEKVATYLFRTECGDLVNVL 120
           ESRAGFAKSSRYNLSKAYASFGISVEKNGWRFGISGKSKEQEKVATYLFRTECGDLVNVL
Sbjct: 61  ESRAGFAKSSRYNLSKAYASFGISVEKNGWRFGISGKSKEQEKVATYLFRTECGDLVNVL 120

Query: 121 VRKTGSGFMVSIELSSVQLANIEETPMLSWGVYRSDSALVTPNIQSSLPDDTIGTIETPF 180
           VRKTGSGFMVSIELSSVQLANIEETPMLSWGVYRSDSALVTPNIQSSLPDDTIGTIETPF
Sbjct: 121 VRKTGSGFMVSIELSSVQLANIEETPMLSWGVYRSDSALVTPNIQSSLPDDTIGTIETPF 180

Query: 181 VNTSEGKFAVELEFDAKHVPFYLSFLLKHPVGVDSGSSEIRSHRKTSFCVPVGFGRGYPS 240
           VNTSEGKFAVELEFDAKHVPFYLSFLLKHPVGVDSGSSEIRSHRKTSFCVPVGFGRGYPS
Sbjct: 181 VNTSEGKFAVELEFDAKHVPFYLSFLLKHPVGVDSGSSEIRSHRKTSFCVPVGFGRGYPS 240

Query: 241 PLGVSISGDGSMNFSIFSSSAESVVLCLYNDSTSEKPVIELDLDRYINRSGNIWHVSFES 300
           PLGVSISGDGSMNFSIFSSSAESVVLCLYNDSTSEKPVIELDLDRYINRSGNIWHVSFES
Sbjct: 241 PLGVSISGDGSMNFSIFSSSAESVVLCLYNDSTSEKPVIELDLDRYINRSGNIWHVSFES 300

Query: 301 VSNFVSYGYLCKGSKSHKKQDGVEISRILVDPYAKVLTTSSHKTHGHGLGLPSKLLGQIC 360
           VSNFVSYGYLCKGSKSHKKQDGVEISRILVDPYAKVLTTSSHKTHGHGLGLPSKLLGQIC
Sbjct: 301 VSNFVSYGYLCKGSKSHKKQDGVEISRILVDPYAKVLTTSSHKTHGHGLGLPSKLLGQIC 360

Query: 361 KVPAFDWDGEVRPNLAMEKLFTYRLNVKRFTVDKSSQLTTDIAGTFAGLTKKLLHFKNLG 420
           KVPAFDWDGEVRPNLAMEKLFTYRLNVKRFTVDKSSQLTTDIAGTFAGLTKKLLHFKNLG
Sbjct: 361 KVPAFDWDGEVRPNLAMEKLFTYRLNVKRFTVDKSSQLTTDIAGTFAGLTKKLLHFKNLG 420

Query: 421 VNAVLLEPIFPFDEKEGPYFPSHFFSPTNIYGPSGGSISAINSMKEMVKELHANGIEVML 480
           VNAVLLEPIFPFDEKEGPYFPSHFFSPTNIYGPSGGSISAINSMKEMVKELHANGIEVML
Sbjct: 421 VNAVLLEPIFPFDEKEGPYFPSHFFSPTNIYGPSGGSISAINSMKEMVKELHANGIEVML 480

Query: 481 EVVFTNTAEDGTLQGIDDSSYYFTNRVANLEGKNALNCNYPVVQQLLLDSLRYWVTEFHI 540
           EVVFTNTAEDGTLQGIDDSSYYFTNRVANLEGKNALNCNYPVVQQLLLDSLRYWVTEFHI
Sbjct: 481 EVVFTNTAEDGTLQGIDDSSYYFTNRVANLEGKNALNCNYPVVQQLLLDSLRYWVTEFHI 540

Query: 541 DGFCFINASSLLRGYRGELLSRPPLVEAIAFDPLLSKTKIIADFWDPHESISKETRFPHW 600
           DGFCFINASSLLRGYRGELLSRPPLVEAIAFDPLLSKTKIIADFWDPHESISKETRFPHW
Sbjct: 541 DGFCFINASSLLRGYRGELLSRPPLVEAIAFDPLLSKTKIIADFWDPHESISKETRFPHW 600

Query: 601 KRWAEVNYKFCSDVRDFFRGEGLVSSLATRLCGSGDIFSDGRGPAFSFNFIARNFGLPLV 660
           KRWAEVNYKFCSDVRDFFRGEGLVSSLATRLCGSGDIFSDGRGPAFSFNFIARNFGLPLV
Sbjct: 601 KRWAEVNYKFCSDVRDFFRGEGLVSSLATRLCGSGDIFSDGRGPAFSFNFIARNFGLPLV 660

Query: 661 DLVSFSSSKLASELSWNCGEEGPTSNIEVLEKRLKLIRNFIFVLFVSLGVPVLNMGDECG 720
           DLVSFSSSKLASELSWNCGEEGPTSNIEVLEKRLKLIRNFIFVLFVSLGVPVLNMGDECG
Sbjct: 661 DLVSFSSSKLASELSWNCGEEGPTSNIEVLEKRLKLIRNFIFVLFVSLGVPVLNMGDECG 720

Query: 721 QSSGGSIAFKDRRPFDWDLLKTGFGTQTTQFITFFSSFRARRFDLFQSRNFLKGENIDWY 780
           QSSGGSIAFKDRRPFDWDLLKTGFGTQTTQFITFFSSFRARRFDLFQSRNFLKGENIDWY
Sbjct: 721 QSSGGSIAFKDRRPFDWDLLKTGFGTQTTQFITFFSSFRARRFDLFQSRNFLKGENIDWY 780

Query: 781 GNNLSPPQWEDPSCKFLAVMLRADKEENGLSAENPEPQSDIFMAFNASDQSINVALPEPS 840
           GNNLSPPQWEDPSCKFLAVMLRADKEENGLSAENPEPQSDIFMAFNASDQSINVALPEPS
Sbjct: 781 GNNLSPPQWEDPSCKFLAVMLRADKEENGLSAENPEPQSDIFMAFNASDQSINVALPEPS 840

Query: 841 EGTSWFRLIDTALSFPGFFSSDGELVPTVESVTYEMQAHSCALFEAKSYSD 892
           EGTSWFRLIDTALSFPGFFSSDGELVPTVESVTYEMQAHSCALFEAKSYSD
Sbjct: 841 EGTSWFRLIDTALSFPGFFSSDGELVPTVESVTYEMQAHSCALFEAKSYSD 891

BLAST of Moc09g07920 vs. NCBI nr
Match: XP_038898155.1 (isoamylase 2, chloroplastic [Benincasa hispida])

HSP 1 Score: 1513.0 bits (3916), Expect = 0.0e+00
Identity = 740/885 (83.62%), Postives = 796/885 (89.94%), Query Frame = 0

Query: 7   MATFPSSLVVQPYNVHNFGVTEAFRLAASDHHTHGHMIKHQSGKRDEVRMLAHGESRAGF 66
           MATF SSLV+QP+ V+N GVTE+F+LA  DHHT+G  IK+ SGK DE RMLAHGESR G 
Sbjct: 1   MATFLSSLVIQPFCVYNSGVTESFKLAVGDHHTYGQKIKYHSGKMDEARMLAHGESRVGS 60

Query: 67  AKSSRYNLSKAYASFGISVEKNGWRFGISGKSKEQEKVATYLFRTECGDLVNVLVRKTGS 126
            KSS  NLSK YA+ GI V K+G RFG  GKSKE  KVATYLFRTE GDLVNV V KTGS
Sbjct: 61  TKSSHRNLSKVYATSGILVGKSGQRFGTGGKSKEHVKVATYLFRTEFGDLVNVFVGKTGS 120

Query: 127 GFMVSIELSSVQLANIEETPMLSWGVYRSDSALVTPNIQSSLPDDTIGTIETPFVNTSEG 186
           GFMV++E+SSVQLA+IE+T +LSWGVYRSDSA+VTPN QSSLPD+TIG  ETPFV TS G
Sbjct: 121 GFMVNVEISSVQLASIEKTLLLSWGVYRSDSAIVTPNFQSSLPDETIGATETPFVKTSGG 180

Query: 187 KFAVELEFDAKHVPFYLSFLLKHPVGVDSGSSEIRSHRKTSFCVPVGFGRGYPSPLGVSI 246
           KFAVELEFDAKH PFYLSFLLK+PVGVDSGSSEIRSHRKTSF VPVGFGRGYPSPLG+SI
Sbjct: 181 KFAVELEFDAKHTPFYLSFLLKYPVGVDSGSSEIRSHRKTSFTVPVGFGRGYPSPLGLSI 240

Query: 247 SGDGSMNFSIFSSSAESVVLCLYNDSTSEKPVIELDLDRYINRSGNIWHVSFESVSNFVS 306
           SGDGS+NFSIFSS+AESVVLCLYNDSTSEKPV+ELDLD YINRSG+IWH SFES SNFVS
Sbjct: 241 SGDGSINFSIFSSTAESVVLCLYNDSTSEKPVLELDLDSYINRSGSIWHASFESASNFVS 300

Query: 307 YGYLCKGSKSHKKQDGVEISRILVDPYAKVLTTSSHKTHGHGLGLPSKLLGQICKVPAFD 366
           YGY CKGSK H+ QDGVE++RI+VDPYAK+LT S  KT G GLGLP K LGQICKVPAFD
Sbjct: 301 YGYRCKGSKFHENQDGVEVNRIVVDPYAKILTASVAKTLGQGLGLP-KFLGQICKVPAFD 360

Query: 367 WDGEVRPNLAMEKLFTYRLNVKRFTVDKSSQLTTDIAGTFAGLTKKLLHFKNLGVNAVLL 426
           WDGEV PNL MEKLF YRLNVKRFTVDKSSQL  DIAGTF+GLTKKLLHFKNLGVNAVLL
Sbjct: 361 WDGEVCPNLPMEKLFAYRLNVKRFTVDKSSQLPADIAGTFSGLTKKLLHFKNLGVNAVLL 420

Query: 427 EPIFPFDEKEGPYFPSHFFSPTNIYGPSGGSISAINSMKEMVKELHANGIEVMLEVVFTN 486
           EPIF FDEKEG YFP HFFSP N YGPSG SISAINSMK MVK+LHANG+EV+LEVVFT+
Sbjct: 421 EPIFQFDEKEGLYFPFHFFSPANNYGPSGVSISAINSMKGMVKQLHANGVEVILEVVFTH 480

Query: 487 TAEDGTLQGIDDSSYYFTNRVANLEGKNALNCNYPVVQQLLLDSLRYWVTEFHIDGFCFI 546
           TA DG LQGIDDSSYYF NRVANLEGK+ALNCNYP+VQQLLLDSLRYWVTEFHIDGFCFI
Sbjct: 481 TAMDGALQGIDDSSYYFVNRVANLEGKSALNCNYPIVQQLLLDSLRYWVTEFHIDGFCFI 540

Query: 547 NASSLLRGYRGELLSRPPLVEAIAFDPLLSKTKIIADFWDPHESISKETRFPHWKRWAEV 606
           NASSLLRG+ GE LSR P VEAIAFDPLLSKTK++ADFWDP E +S+ET FPHWKRWAEV
Sbjct: 541 NASSLLRGHHGEFLSRSPFVEAIAFDPLLSKTKLVADFWDPQELVSEETSFPHWKRWAEV 600

Query: 607 NYKFCSDVRDFFRGEGLVSSLATRLCGSGDIFSDGRGPAFSFNFIARNFGLPLVDLVSFS 666
           N KFC+DVRDFFRGEGL+SSLATRLCGSGD+FS+GRGPAFSFNFI RN GLPLVDLVSFS
Sbjct: 601 NSKFCNDVRDFFRGEGLISSLATRLCGSGDVFSNGRGPAFSFNFITRNVGLPLVDLVSFS 660

Query: 667 SSKLASELSWNCGEEGPTSNIEVLEKRLKLIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 726
           +SKLASELSWNCGEEGPTSNIEVLEKRLK IRNFIFV+FVSLGVPVLNMGDECGQS+GGS
Sbjct: 661 NSKLASELSWNCGEEGPTSNIEVLEKRLKQIRNFIFVVFVSLGVPVLNMGDECGQSAGGS 720

Query: 727 IAFKDRRPFDWDLLKTGFGTQTTQFITFFSSFRARRFDLFQSRNFLKGENIDWYGNNLSP 786
           +AF DRR F+WDLLKT FGTQTTQFITF SSFR+RRFDLFQSRNFLKGENIDW+ NN SP
Sbjct: 721 VAFNDRRSFNWDLLKTDFGTQTTQFITFLSSFRSRRFDLFQSRNFLKGENIDWFDNNQSP 780

Query: 787 PQWEDPSCKFLAVMLRADKEENGLSAENPEPQSDIFMAFNASDQSINVALPEPSEGTSWF 846
           PQWEDPSCKFLAVMLRADKEENG   EN +P+SDIFMAFNASDQS  VALPEPSEGTSWF
Sbjct: 781 PQWEDPSCKFLAVMLRADKEENGSITENAKPRSDIFMAFNASDQSETVALPEPSEGTSWF 840

Query: 847 RLIDTALSFPGFFSSDGELVPTVESVTYEMQAHSCALFEAKSYSD 892
           RL+DTALSFPGFFSSDGE VPT  SVTYEMQAHSCALFEAKS++D
Sbjct: 841 RLVDTALSFPGFFSSDGEQVPTTGSVTYEMQAHSCALFEAKSFND 884

BLAST of Moc09g07920 vs. NCBI nr
Match: KAG7014422.1 (Isoamylase 2, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1479.2 bits (3828), Expect = 0.0e+00
Identity = 718/890 (80.67%), Postives = 795/890 (89.33%), Query Frame = 0

Query: 2   LLQPQMATFPSSLVVQPYNVHNFGVTEAFRLAASDHHTHGHMIKHQSGKRDEVRMLAHGE 61
           L Q QMATF SSL +QP+  +N G TE+F+LA +D+HT+G  + +QSGK DE RM AHGE
Sbjct: 19  LYQFQMATFLSSL-IQPFCGYNRGGTESFKLAVNDYHTYGQKMNYQSGKLDEARMQAHGE 78

Query: 62  SRAGFAKSSRYNLSKAYASFGISVEKNGWRFGISGKSKEQEKVATYLFRTECGDLVNVLV 121
           S+ GF KS   N+ KAYA  GISV K+G +FG  GK++E  KVATYLFRTE GDLV   V
Sbjct: 79  SKVGFTKSLHRNVCKAYAPSGISVGKSGQKFGTGGKTEEDRKVATYLFRTEFGDLVYAFV 138

Query: 122 RKTGSGFMVSIELSSVQLANIEETPMLSWGVYRSDSALVTPNIQSSLPDDTIGTIETPFV 181
            K GSGF+VSIE+SS++LA+I+ET +LSWGVYRS+SA+VTPN QS LP++TIG  ETPFV
Sbjct: 139 GKRGSGFLVSIEISSLKLASIDETLLLSWGVYRSNSAIVTPNFQSILPEETIGATETPFV 198

Query: 182 NTSEGKFAVELEFDAKHVPFYLSFLLKHPVGVDSGSSEIRSHRKTSFCVPVGFGRGYPSP 241
            TSEGKFAVELEFDAKH PFYLSFLLK+PVGVDSGSSEI+SHRKTSF VPVGFGRGYPSP
Sbjct: 199 RTSEGKFAVELEFDAKHDPFYLSFLLKYPVGVDSGSSEIKSHRKTSFSVPVGFGRGYPSP 258

Query: 242 LGVSISGDGSMNFSIFSSSAESVVLCLYNDSTSEKPVIELDLDRYINRSGNIWHVSFESV 301
           LG+SISGDGS+NFSIFSS+AESVVLCLYNDSTSEKPV+ELDLD YINRSGNIWHVSFE  
Sbjct: 259 LGLSISGDGSINFSIFSSTAESVVLCLYNDSTSEKPVLELDLDPYINRSGNIWHVSFEGA 318

Query: 302 SNFVSYGYLCKGSKSHKKQDGVEISRILVDPYAKVLTTSSHKTHGHGLGLPSKLLGQICK 361
           SNFVSYGY C+GSK H+ QD  E+SRILVDPYAK+LT S+ KT G G+GLPSK LGQIC 
Sbjct: 319 SNFVSYGYRCRGSKFHENQDEAEVSRILVDPYAKILTASTPKTRGQGVGLPSKFLGQICN 378

Query: 362 VPAFDWDGEVRPNLAMEKLFTYRLNVKRFTVDKSSQLTTDIAGTFAGLTKKLLHFKNLGV 421
           VPAF WDGEVRPNL MEKLF YRLNVK FTVDKSSQL  DIAGTF+GLT+KLLHFK LGV
Sbjct: 379 VPAFSWDGEVRPNLPMEKLFIYRLNVKHFTVDKSSQLPIDIAGTFSGLTQKLLHFKKLGV 438

Query: 422 NAVLLEPIFPFDEKEGPYFPSHFFSPTNIYGPSGGSISAINSMKEMVKELHANGIEVMLE 481
           NAVLLEPIFPFDEK+GPYFP HFFSPTNIY PSG SIS++NS+KEMVKELHANG+EV+LE
Sbjct: 439 NAVLLEPIFPFDEKDGPYFPFHFFSPTNIYVPSGASISSLNSVKEMVKELHANGVEVILE 498

Query: 482 VVFTNTAEDGTLQGIDDSSYYFTNRVANLEGKNALNCNYPVVQQLLLDSLRYWVTEFHID 541
           VVFT+TA DG LQGIDDS YYF NRVAN EGK+ALNCN+P+VQQLLLDSLRYWVTEFHID
Sbjct: 499 VVFTHTAMDGALQGIDDSCYYFVNRVANSEGKSALNCNHPIVQQLLLDSLRYWVTEFHID 558

Query: 542 GFCFINASSLLRGYRGELLSRPPLVEAIAFDPLLSKTKIIADFWDPHESISKETRFPHWK 601
           GFCFINASSLL+G+ GELLSRPP VEAIAFDPLLSKTKIIA+FWDP E + KETRFPHWK
Sbjct: 559 GFCFINASSLLQGHHGELLSRPPFVEAIAFDPLLSKTKIIAEFWDPQELVPKETRFPHWK 618

Query: 602 RWAEVNYKFCSDVRDFFRGEGLVSSLATRLCGSGDIFSDGRGPAFSFNFIARNFGLPLVD 661
           RWAE+N KF +D+++FFRGEGLVSSLATRLCGSGD+FSDGRGPAFSFNFIARN GLPLVD
Sbjct: 619 RWAELNSKFSNDMKEFFRGEGLVSSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVD 678

Query: 662 LVSFSSSKLASELSWNCGEEGPTSNIEVLEKRLKLIRNFIFVLFVSLGVPVLNMGDECGQ 721
           LVSFSSSKLASELSWNCGEEGPTSNIEVLEKRLK IRNFIFVLFVSLGVPVLNMGDECGQ
Sbjct: 679 LVSFSSSKLASELSWNCGEEGPTSNIEVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQ 738

Query: 722 SSGGSIAFKDRRPFDWDLLKTGFGTQTTQFITFFSSFRARRFDLFQSRNFLKGENIDWYG 781
           SSGGS+AFKDR+ F+WD LKT FGTQTT+FI FFSSFR+RRFDLFQSR+FLKGENIDW+ 
Sbjct: 739 SSGGSVAFKDRKSFNWDSLKTDFGTQTTRFIAFFSSFRSRRFDLFQSRHFLKGENIDWFD 798

Query: 782 NNLSPPQWEDPSCKFLAVMLRADKEENGLSAENPEPQSDIFMAFNASDQSINVALPEPSE 841
           NN SPP+WEDPSCKFLAVMLRADKEE+G   ENP+PQSDIFMAFNASDQS++VALPEPSE
Sbjct: 799 NNQSPPEWEDPSCKFLAVMLRADKEESGSITENPKPQSDIFMAFNASDQSVSVALPEPSE 858

Query: 842 GTSWFRLIDTALSFPGFFSSDGELVPTVESVTYEMQAHSCALFEAKSYSD 892
           GT+WFRL+DTALSFPGFFSSDGE VPT   VTYEMQAHSC LFEAKS++D
Sbjct: 859 GTNWFRLVDTALSFPGFFSSDGEPVPTTSPVTYEMQAHSCTLFEAKSFND 907

BLAST of Moc09g07920 vs. NCBI nr
Match: XP_004136188.1 (isoamylase 2, chloroplastic [Cucumis sativus] >KGN44865.1 hypothetical protein Csa_016566 [Cucumis sativus])

HSP 1 Score: 1469.1 bits (3802), Expect = 0.0e+00
Identity = 713/885 (80.56%), Postives = 784/885 (88.59%), Query Frame = 0

Query: 7   MATFPSSLVVQPYNVHNFGVTEAFRLAASDHHTHGHMIKHQSGKRDEVRMLAHGESRAGF 66
           MATF SSLV+QP+ V+N GVTE+ +LAASDH T+G   K+Q GK DE RMLAHGE++ G 
Sbjct: 1   MATFLSSLVIQPFCVYNCGVTESPKLAASDHLTYGQKTKYQFGKMDEARMLAHGENKVGA 60

Query: 67  AKSSRYNLSKAYASFGISVEKNGWRFGISGKSKEQEKVATYLFRTECGDLVNVLVRKTGS 126
            KSS  NLSK YA  GISV K+G R GI GKSKEQ +VATYLFRTE GDLVNV V K GS
Sbjct: 61  VKSSHRNLSKTYAKSGISVGKSGQRLGIGGKSKEQRRVATYLFRTEFGDLVNVFVGKKGS 120

Query: 127 GFMVSIELSSVQLANIEETPMLSWGVYRSDSALVTPNIQSSLPDDTIGTIETPFVNTSEG 186
            F V+IE+ S+QL +I+E  +LSWGVYRSDSALVTPN +SS PD+T G  ETPFV TSEG
Sbjct: 121 TFTVNIEVPSMQLVSIDEALLLSWGVYRSDSALVTPNFESSPPDETTGATETPFVKTSEG 180

Query: 187 KFAVELEFDAKHVPFYLSFLLKHPVGVDSGSSEIRSHRKTSFCVPVGFGRGYPSPLGVSI 246
           KF+VELEFDAKH PFYLSF+LK+P+GVDSGSSEIRSH+KTSF VPVGFGRGYPSPLG+SI
Sbjct: 181 KFSVELEFDAKHTPFYLSFVLKYPMGVDSGSSEIRSHKKTSFSVPVGFGRGYPSPLGLSI 240

Query: 247 SGDGSMNFSIFSSSAESVVLCLYNDSTSEKPVIELDLDRYINRSGNIWHVSFESVSNFVS 306
           SGDGS+NFSIFSSSAES+VLCLYNDSTSEKP++ELDLD YINRSGNIWH SFE  S FVS
Sbjct: 241 SGDGSVNFSIFSSSAESLVLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGASKFVS 300

Query: 307 YGYLCKGSKSHKKQDGVEISRILVDPYAKVLTTSSHKTHGHGLGLPSKLLGQICKVPAFD 366
           YGY CKGSKSH+ QDG+E+SRI+VDPYAK+L  S  K+ G GLGLPSK LGQI KVP FD
Sbjct: 301 YGYQCKGSKSHENQDGLEVSRIVVDPYAKILAPSIPKSSGQGLGLPSKFLGQISKVPTFD 360

Query: 367 WDGEVRPNLAMEKLFTYRLNVKRFTVDKSSQLTTDIAGTFAGLTKKLLHFKNLGVNAVLL 426
           WDGEV PNL MEKLF YRLNV+RFT+DKSSQL  DIAGTF+GLTKKLLHFKNLGVNAVLL
Sbjct: 361 WDGEVHPNLPMEKLFVYRLNVERFTMDKSSQLPADIAGTFSGLTKKLLHFKNLGVNAVLL 420

Query: 427 EPIFPFDEKEGPYFPSHFFSPTNIYGPSGGSISAINSMKEMVKELHANGIEVMLEVVFTN 486
           EPIF FDEKEGPYFP HFFSPTN YGPSG SISAINSMKEMVKELHANG+EV+LEVV+T+
Sbjct: 421 EPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVILEVVYTH 480

Query: 487 TAEDGTLQGIDDSSYYFTNRVANLEGKNALNCNYPVVQQLLLDSLRYWVTEFHIDGFCFI 546
           T+ +G LQGIDDSSYYFTNRVANLE K+ALNCNYP+VQQLLLDSLRYWVTEFH+DGFCF+
Sbjct: 481 TSGNGALQGIDDSSYYFTNRVANLEEKSALNCNYPIVQQLLLDSLRYWVTEFHVDGFCFV 540

Query: 547 NASSLLRGYRGELLSRPPLVEAIAFDPLLSKTKIIADFWDPHESISKETRFPHWKRWAEV 606
           NAS LLRG+ GELLSRPP VEAIAFDPLLSKTK++ADFWDP E  SKETRFPHWKRWAEV
Sbjct: 541 NASFLLRGHHGELLSRPPFVEAIAFDPLLSKTKLVADFWDPQELESKETRFPHWKRWAEV 600

Query: 607 NYKFCSDVRDFFRGEGLVSSLATRLCGSGDIFSDGRGPAFSFNFIARNFGLPLVDLVSFS 666
           N KFCSD+RDFFRGEGL+SSLATRLCGSGD+FSDGRGPAFSFNFIARN GLPLVDLVSFS
Sbjct: 601 NSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS 660

Query: 667 SSKLASELSWNCGEEGPTSNIEVLEKRLKLIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 726
           +S LASELSWNCGEEGPTSN++VLEKRLK IRNFIFVLFVSLGVPVLNMGDECGQSSGGS
Sbjct: 661 NSNLASELSWNCGEEGPTSNLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 720

Query: 727 IAFKDRRPFDWDLLKTGFGTQTTQFITFFSSFRARRFDLFQSRNFLKGENIDWYGNNLSP 786
           +AF D+R F+WDLLKT FGTQTTQFI F SSFR+RRFDLFQ+RNFLKGENIDW+ NN SP
Sbjct: 721 VAFNDKRSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLFQNRNFLKGENIDWFDNNQSP 780

Query: 787 PQWEDPSCKFLAVMLRADKEENGLSAENPEPQSDIFMAFNASDQSINVALPEPSEGTSWF 846
           PQWED SCKFLAVMLRADKEEN    ENP+ +S+IFM FNASDQS +VALPEP EGTSWF
Sbjct: 781 PQWEDASCKFLAVMLRADKEENESITENPKTRSNIFMVFNASDQSESVALPEPLEGTSWF 840

Query: 847 RLIDTALSFPGFFSSDGELVPTVESVTYEMQAHSCALFEAKSYSD 892
           R++DTAL FPGFFSSDGELVP   SVTYE+QAHSCALFEAKS +D
Sbjct: 841 RVVDTALPFPGFFSSDGELVPMTGSVTYEIQAHSCALFEAKSAND 885

BLAST of Moc09g07920 vs. NCBI nr
Match: KAA0057598.1 (isoamylase 2 [Cucumis melo var. makuwa] >TYK20996.1 isoamylase 2 [Cucumis melo var. makuwa])

HSP 1 Score: 1468.4 bits (3800), Expect = 0.0e+00
Identity = 714/882 (80.95%), Postives = 778/882 (88.21%), Query Frame = 0

Query: 7   MATFPSSLVVQPYNVHNFGVTEAFRLAASDHHTHGHMIKHQSGKRDEVRMLAHGESRAGF 66
           MATF SSLV+QP+ VHN GVTE+ +LA SDH T+G   K+QSGK DE RMLAHGES+ G 
Sbjct: 1   MATFLSSLVIQPFCVHNCGVTESLKLAVSDHLTYGQKTKYQSGKMDEARMLAHGESKVGA 60

Query: 67  AKSSRYNLSKAYASFGISVEKNGWRFGISGKSKEQEKVATYLFRTECGDLVNVLVRKTGS 126
            KSS  NLSKAY + GISV K+G R GI GKSKEQ KVATYLFRTE GDLVNV V KTGS
Sbjct: 61  VKSSHRNLSKAYVTSGISVGKSGQRLGIGGKSKEQRKVATYLFRTEFGDLVNVFVGKTGS 120

Query: 127 GFMVSIELSSVQLANIEETPMLSWGVYRSDSALVTPNIQSSLPDDTIGTIETPFVNTSEG 186
            F V+IE+SS+QL + +ET +LSW VYRSDSA VT N QSS PD T G  ETPFV TSEG
Sbjct: 121 AFTVNIEISSMQLVSTDETLLLSWRVYRSDSAPVTSNFQSSPPDGTTGATETPFVKTSEG 180

Query: 187 KFAVELEFDAKHVPFYLSFLLKHPVGVDSGSSEIRSHRKTSFCVPVGFGRGYPSPLGVSI 246
           KF+VELEFDAKH PFYLSF+LK P+GVDSGSSEIRSHRKTSF VPVGFGRGYPSP G+SI
Sbjct: 181 KFSVELEFDAKHTPFYLSFVLKFPMGVDSGSSEIRSHRKTSFSVPVGFGRGYPSPSGLSI 240

Query: 247 SGDGSMNFSIFSSSAESVVLCLYNDSTSEKPVIELDLDRYINRSGNIWHVSFESVSNFVS 306
           SGDGS+NFSIFSS+AESVVLCLYNDSTSEKP++ELDLD YINRSGNIWH SFE VSNFVS
Sbjct: 241 SGDGSINFSIFSSTAESVVLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGVSNFVS 300

Query: 307 YGYLCKGSKSHKKQDGVEISRILVDPYAKVLTTSSHKTHGHGLGLPSKLLGQICKVPAFD 366
           YGY CKG KSH+ QDG+E+SRI+VDPYAK+L  S  K+   GLG P K LGQI  VPAFD
Sbjct: 301 YGYQCKGLKSHENQDGLEVSRIVVDPYAKILAPSIPKSRDQGLGFPLKFLGQISIVPAFD 360

Query: 367 WDGEVRPNLAMEKLFTYRLNVKRFTVDKSSQLTTDIAGTFAGLTKKLLHFKNLGVNAVLL 426
           WDGEVRPNL MEKLF YRLNVKRFT+DKSSQL  DIAGTF+GLTKKLLH KNLGVNAVLL
Sbjct: 361 WDGEVRPNLPMEKLFVYRLNVKRFTIDKSSQLPADIAGTFSGLTKKLLHLKNLGVNAVLL 420

Query: 427 EPIFPFDEKEGPYFPSHFFSPTNIYGPSGGSISAINSMKEMVKELHANGIEVMLEVVFTN 486
           EPIF FDEKEGPYFP HFFSPTN YGPSG SISAINSMKEMVKELHANG+EV++EVV+T+
Sbjct: 421 EPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVIVEVVYTH 480

Query: 487 TAEDGTLQGIDDSSYYFTNRVANLEGKNALNCNYPVVQQLLLDSLRYWVTEFHIDGFCFI 546
           T+ DG LQGIDDSSYYF NRVANLE ++ALNCNYP+VQQLLLDSLRYWVTEFH+DGFCF+
Sbjct: 481 TSVDGALQGIDDSSYYFANRVANLEERSALNCNYPIVQQLLLDSLRYWVTEFHVDGFCFV 540

Query: 547 NASSLLRGYRGELLSRPPLVEAIAFDPLLSKTKIIADFWDPHESISKETRFPHWKRWAEV 606
           NAS LL+G+ GELLSRPP VEAIAFDP+LSKTK++ADFWDP E +SKETRFPHWKRWAEV
Sbjct: 541 NASFLLQGHHGELLSRPPFVEAIAFDPILSKTKLVADFWDPQELVSKETRFPHWKRWAEV 600

Query: 607 NYKFCSDVRDFFRGEGLVSSLATRLCGSGDIFSDGRGPAFSFNFIARNFGLPLVDLVSFS 666
           N KFCSD+RDFFRGEGL+SSLATRLCGSGD+FSDGRGPAFSFNFIARN GLPLVDLVSFS
Sbjct: 601 NSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS 660

Query: 667 SSKLASELSWNCGEEGPTSNIEVLEKRLKLIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 726
           +S LASELSWNCGEEGPT+ ++VLEKRLK IRNFIFVLFVSLGVPVLNMGDECGQSSGGS
Sbjct: 661 NSNLASELSWNCGEEGPTNTLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 720

Query: 727 IAFKDRRPFDWDLLKTGFGTQTTQFITFFSSFRARRFDLFQSRNFLKGENIDWYGNNLSP 786
           +AF DRR F+WDLLKT FGTQTTQFI F SSFR+RRFDLFQSRNFLKGENIDW+ NN SP
Sbjct: 721 VAFNDRRSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLFQSRNFLKGENIDWFDNNQSP 780

Query: 787 PQWEDPSCKFLAVMLRADKEENGLSAENPEPQSDIFMAFNASDQSINVALPEPSEGTSWF 846
           PQWED SCKFLAVMLRADKEEN    ENP+ +SDIFMAFNASDQS +VALPEPSEGTSWF
Sbjct: 781 PQWEDASCKFLAVMLRADKEENESITENPKTRSDIFMAFNASDQSESVALPEPSEGTSWF 840

Query: 847 RLIDTALSFPGFFSSDGELVPTVESVTYEMQAHSCALFEAKS 889
           R++DTAL FPGFFSSDGELVP   SVTYEMQAHSCALFEAKS
Sbjct: 841 RVVDTALPFPGFFSSDGELVPMTGSVTYEMQAHSCALFEAKS 882

BLAST of Moc09g07920 vs. ExPASy Swiss-Prot
Match: Q8L735 (Isoamylase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ISA2 PE=1 SV=2)

HSP 1 Score: 924.5 bits (2388), Expect = 9.3e-268
Identity = 459/824 (55.70%), Postives = 595/824 (72.21%), Query Frame = 0

Query: 72  YNLSKAYASFGISVEKNGWRFGISGKSKEQEKVATYLFRTECGDLVNVLVRKTGSGFMVS 131
           Y  SK  AS   S+E          ++ + +KV +Y FRT+ G LV V V K    + + 
Sbjct: 64  YQFSKICAS-KTSIELREALSSRRAEADDLKKVTSYSFRTKAGALVKVKVEKKREKYSIL 123

Query: 132 IELSSVQLANIEETPM-LSWGVYRSDSALVTP-NIQSSLPDDTIGTIETPFVNTSEGKFA 191
           + +SS++L+  +++ + + WGVYRSDS+   P + ++S  D    T ET FV +S  +  
Sbjct: 124 VYVSSLELSGDDKSRLVMVWGVYRSDSSCFLPLDFENSSQDSQTHTTETTFVKSSLSELM 183

Query: 192 VELEFDAKHVPFYLSFLLKHPVGVDSGSSEIRSHRKTSFCVPVGFGRGYPSPLGVSIS-G 251
           + LEFD K  PFYLSF LK   G D    E+ +HR T FC+PVGF  G+P PLG+S    
Sbjct: 184 LGLEFDGKESPFYLSFHLKLVSGRDPDGQEMLTHRDTDFCIPVGFTAGHPLPLGLSSGPD 243

Query: 252 DGSMNFSIFSSSAESVVLCLYNDSTSEKPVIELDLDRYINRSGNIWHVSFESVSNFVSYG 311
           D S NFS FS S+ +VVLCLY+DST++KP +ELDLD Y+NR+G++WH S ++  +FV YG
Sbjct: 244 DDSWNFSFFSRSSTNVVLCLYDDSTTDKPALELDLDPYVNRTGDVWHASVDNTWDFVRYG 303

Query: 312 YLCKGSKSHKKQDGVEISRILVDPYAKVLTTSSHKTHGHGLGLPSKLLGQICKVPAFDWD 371
           Y CK +   K+   VE   I++DPYA V+          G  +  K LG + K P+FDW 
Sbjct: 304 YRCKETAHSKEDVDVEGEPIVLDPYATVV----------GKSVSQKYLGSLSKSPSFDWG 363

Query: 372 GEVRPNLAMEKLFTYRLNVKRFTVDKSSQLTTDIAGTFAGLTKKLLHFKNLGVNAVLLEP 431
            +V PN+ +EKL  YRLNVK FT  +SS+L +++AGTF+G+ +K+ H K LG NAVLLEP
Sbjct: 364 EDVSPNIPLEKLLVYRLNVKGFTQHRSSKLPSNVAGTFSGVAEKVSHLKTLGTNAVLLEP 423

Query: 432 IFPFDEKEGPYFPSHFFSPTNIYGPSGGSISAINSMKEMVKELHANGIEVMLEVVFTNTA 491
           IF F E++GPYFP HFFSP +IYGPS    SA+NSMK MVK+LH+ GIEV+LEVVFT+TA
Sbjct: 424 IFSFSEQKGPYFPFHFFSPMDIYGPSNSLESAVNSMKVMVKKLHSEGIEVLLEVVFTHTA 483

Query: 492 EDGTLQGIDDSSYYFTNRVANLEGKNALNCNYPVVQQLLLDSLRYWVTEFHIDGFCFINA 551
           + G L+GIDDSSYY+  R  +L+ K+ LNCNYPVVQQL+L+SLRYWVTEFH+DGFCFINA
Sbjct: 484 DSGALRGIDDSSYYYKGRANDLDSKSYLNCNYPVVQQLVLESLRYWVTEFHVDGFCFINA 543

Query: 552 SSLLRGYRGELLSRPPLVEAIAFDPLLSKTKIIADFWDPHESISKETRFPHWKRWAEVNY 611
           SSLLRG  GE LSRPPLVEAIAFDPLL++TK+IAD WDP E + KE RFPHWKRWAE+N 
Sbjct: 544 SSLLRGVHGEQLSRPPLVEAIAFDPLLAETKLIADCWDPLEMMPKEVRFPHWKRWAELNT 603

Query: 612 KFCSDVRDFFRGEGLVSSLATRLCGSGDIFSDGRGPAFSFNFIARNFGLPLVDLVSFSSS 671
           ++C +VR+F RG G++S LATR+CGSGD+F+DGRGPAFSFN+I+RN GL LVD+VSFS  
Sbjct: 604 RYCRNVRNFLRGRGVLSDLATRICGSGDVFTDGRGPAFSFNYISRNSGLSLVDIVSFSGP 663

Query: 672 KLASELSWNCGEEGPTSNIEVLEKRLKLIRNFIFVLFVSLGVPVLNMGDECGQSSGGSIA 731
           +LASELSWNCGEEG T+   VL++RLK IRNF+F+ ++SLGVPVLNMGDECG S+ GS  
Sbjct: 664 ELASELSWNCGEEGATNKSAVLQRRLKQIRNFLFIQYISLGVPVLNMGDECGISTRGSPL 723

Query: 732 FKDRRPFDWDLLKTGFGTQTTQFITFFSSFRARRFDLFQSRNFLKGENIDWYGNNLSPPQ 791
            + R+PFDW+LL + FGTQ TQFI+F +S RARR D+FQ R+FLK ENI WY N+ + P+
Sbjct: 724 LESRKPFDWNLLASAFGTQITQFISFMTSVRARRSDVFQRRDFLKPENIVWYANDQTTPK 783

Query: 792 WEDPSCKFLAVMLRADKEENGLS--AENPEPQS-DIFMAFNASDQSINVALPEPSEGTSW 851
           WEDP+ KFLA+ ++++ EE   +  AE  EP+S D+F+ FNASD   +V LP   +G+ W
Sbjct: 784 WEDPASKFLALEIKSESEEEETASLAEPNEPKSNDLFIGFNASDHPESVVLPSLPDGSKW 843

Query: 852 FRLIDTALSFPGFFSSDGELV----PTVESVTYEMQAHSCALFE 886
            RL+DTAL FPGFFS +GE V    P  + V YEM+ +SC LFE
Sbjct: 844 RRLVDTALPFPGFFSVEGETVVAEEPLQQLVVYEMKPYSCTLFE 876

BLAST of Moc09g07920 vs. ExPASy Swiss-Prot
Match: Q6AU80 (Isoamylase 2, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=ISA2 PE=1 SV=1)

HSP 1 Score: 769.6 bits (1986), Expect = 3.8e-221
Identity = 383/717 (53.42%), Postives = 510/717 (71.13%), Query Frame = 0

Query: 189 AVELEFDAKHVPFYLSFLLKHPVGVDSGSSEIRSHRKTSFCVPVGFGRGYPSPLGVSISG 248
           A EL FD    PFYLSFLL      D+  +EIR+HRKTSF VPVG G G P+PLG+SISG
Sbjct: 105 AAELSFDPTRAPFYLSFLL-----TDASGAEIRTHRKTSFRVPVGVGPGSPAPLGMSISG 164

Query: 249 DGSMNFSIFSSSAESVVLCLY----NDSTSEKPVIELDLDRYINRSGNIWHVSFESVSNF 308
           DG++NF+++S +A +V L LY         ++P +E+DLD YI+R+GN+WHVS  SV  +
Sbjct: 165 DGAVNFAVYSKNANAVSLYLYAAAVGGGGGDEPALEIDLDPYIHRTGNVWHVSLASVDGY 224

Query: 309 VSYGYLCKGSKSHKKQDGVEISRILVDPYAKVL--TTSSHKTHGHGLGLPS-KLLGQICK 368
           VSY + C G           I R L+DPYAKV+    SS+  +  G+  PS +    +  
Sbjct: 225 VSYAFCCGG-----------IRRPLLDPYAKVIGDFVSSNSVYDEGVTAPSMRCFASLAI 284

Query: 369 VPAFDWDGEVRPNLAMEKLFTYRLNVKRFTVDKSSQLTTDIAGTFAGLTKKLLHFKNLGV 428
            P+++W  +  P L +EKL  YR NV  FT D+SS L  D AGTF GL+ K+ HF++LGV
Sbjct: 285 APSYNWGRDRHPRLPLEKLVVYRANVALFTKDRSSGLPDDAAGTFTGLSAKVEHFRSLGV 344

Query: 429 NAVLLEPIFPFDEKEGPYFPSHFFSPTNIYGPSGGSISAINSMKEMVKELHANGIEVMLE 488
           NA+LLEP+FPF + +GPYFP HFFSP N+Y   G S+SAI SMK+MV+ +H NGIEV+LE
Sbjct: 345 NAILLEPVFPFHQVKGPYFPYHFFSPMNLYSSKGLSVSAIKSMKDMVRVMHRNGIEVLLE 404

Query: 489 VVFTNTAEDG------TLQGIDDSSYYFTNRVANLEGKNALNCNYPVVQQLLLDSLRYWV 548
           VVFT+TAE        +++GID+SSYY  N +A  +  + LNCN+PV Q+L+LDSLR+WV
Sbjct: 405 VVFTHTAEGESECQTISMRGIDNSSYYIANGIAGCKA-SILNCNHPVTQKLILDSLRHWV 464

Query: 549 TEFHIDGFCFINASSLLRGYRGELLSRPPLVEAIAFDPLLSKTKIIADFWDPHESISKET 608
            +FH+DGFCFINA  L+RG  GE LSRPPL+EAI FDP+LS TKIIAD W P +  + + 
Sbjct: 465 LDFHVDGFCFINAPFLVRGPGGEYLSRPPLLEAITFDPVLSMTKIIADPWSPLDISNVQF 524

Query: 609 RFPHWKRWAEVNYKFCSDVRDFFRGEGLVSSLATRLCGSGDIFSDGRGPAFSFNFIARNF 668
            FPHWKRWAEVN +F  DVR F + E L+S LATRLCGSGD+FS  RGPAFSFN ++RN 
Sbjct: 525 PFPHWKRWAEVNTRFSIDVRKFLKREALISDLATRLCGSGDLFST-RGPAFSFNHVSRNS 584

Query: 669 GLPLVDLVSFSSSKLASELSWNCGEEGPTSNIEVLEKRLKLIRNFIFVLFVSLGVPVLNM 728
           GL LVDLVSFS+  L SE SWNCGEEGP+ N  VL+ RL+ IRNF+F+LFVSLGVPVLNM
Sbjct: 585 GLSLVDLVSFSNDDLLSESSWNCGEEGPSENSAVLQTRLRQIRNFLFILFVSLGVPVLNM 644

Query: 729 GDECGQSSGGSIAFKDRRPFDWDLLKTGFGTQTTQFITFFSSFRARRFDLFQSRNFLKGE 788
           GDECG S+ GS+++KDR P +W  +KT F  + T FI+F ++ R+RR D+FQ R FLK E
Sbjct: 645 GDECGHSAAGSVSYKDRGPLNWRGMKTTFVKEVTGFISFLTALRSRRGDIFQRREFLKLE 704

Query: 789 NIDWYGNNLSPPQWEDPSCKFLAVMLRADKEENGLSAENPEPQSDIFMAFNASDQSINVA 848
           NI WYG++L  P W+DP+  FL + + A+ +E  ++A++   + D+++ FNA+++S++ A
Sbjct: 705 NIHWYGSDLCEPGWDDPTSNFLCMHINAEVDE--MAADS--VRGDLYICFNANEESVSAA 764

Query: 849 LPEPSEGTSWFRLIDTALSFPGFFSSDGELVPTVESV----TYEMQAHSCALFEAKS 889
           LP  +EG+ W RL+DT+L+FPGFF+++    P V+ V    +Y ++AH+C LFE+KS
Sbjct: 765 LPALAEGSVWLRLVDTSLAFPGFFATESN--PKVQQVPGLSSYHVEAHTCVLFESKS 797

BLAST of Moc09g07920 vs. ExPASy Swiss-Prot
Match: Q9M0S5 (Isoamylase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ISA3 PE=1 SV=2)

HSP 1 Score: 375.2 bits (962), Expect = 2.1e-102
Identity = 254/704 (36.08%), Postives = 363/704 (51.56%), Query Frame = 0

Query: 237 GYPSPLGVSISGDGSMNFSIFSSSAESVVLCLY-----NDSTSEKPVIELDLDRYINRSG 296
           G  SPLGVS   D  +NF++FS +A SV LCL       D T +  +IEL LD  +N++G
Sbjct: 88  GEVSPLGVS-QVDKGINFALFSQNATSVTLCLSLSQSGKDDTDDDGMIELVLDPSVNKTG 147

Query: 297 NIWHVSFESVS-NFVSYGYLCKGSKSHKKQDGVEISRILVDPYAKVLTTSSHKTHGHGLG 356
           + WH+  E +  N V YGY   G    ++    + S +L+DPYAK++    H + G    
Sbjct: 148 DTWHICVEDLPLNNVLYGYRVDGPGEWQQGHRFDRSILLLDPYAKLV--KGHSSFGDSSQ 207

Query: 357 LPSKLLGQI-CKVPAFDWDGEVR-PNLAMEKLFTYRLNVKRFTVDKSSQLTTDIAGTFAG 416
             ++  G    +   FDW  + + PN+  + L  Y +NV+ FT D+SS +   I G++ G
Sbjct: 208 KFAQFYGTYDFESSPFDWGDDYKFPNIPEKDLVIYEMNVRAFTADESSGMDPAIGGSYLG 267

Query: 417 LTKKLLHFKNLGVNAVLLEPIFPFDEKE--------------GPYFPSHFFSPTNIYGP- 476
             +K+ H ++LG+NAV L P+F FDE E                Y   +FF+P + Y   
Sbjct: 268 FIEKIPHLQDLGINAVELLPVFEFDELELQRRSNPRDHMVNTWGYSTVNFFAPMSRYASG 327

Query: 477 SGGSISAINSMKEMVKELHANGIEVMLEVVF--TNTAED-----GTLQGIDDSSYYF--- 536
            G  I A    KEMVK LH+ GIEV+L+VV+  TN A+D      + +GID+  YY    
Sbjct: 328 EGDPIKASKEFKEMVKALHSAGIEVILDVVYNHTNEADDKYPYTTSFRGIDNKVYYMLDP 387

Query: 537 TNRVANLEG-KNALNCNYPVVQQLLLDSLRYWVTEFHIDGFCFINASSLLRGYRGELLSR 596
            N++ N  G  N LNCN+PVV +L+LDSLR+WVTE+H+DGF F  AS L R   G  LS 
Sbjct: 388 NNQLLNFSGCGNTLNCNHPVVMELILDSLRHWVTEYHVDGFRFDLASVLCRATDGSPLSA 447

Query: 597 PPLVEAIAFDPLLSKTKIIADFWDPHESISKETRFPHWKRWAEVNYKFCSDVRDFFRGE- 656
           PPL+ AIA D +LS+ KIIA+ WD    +    +FP+W RWAE N  +  DVR F +G+ 
Sbjct: 448 PPLIRAIAKDSVLSRCKIIAEPWD-CGGLYLVGKFPNWDRWAEWNGMYRDDVRRFIKGDS 507

Query: 657 GLVSSLATRLCGSGDIFS-DGRGPAFSFNFIARNFGLPLVDLVSFS-----------SSK 716
           G+  S ATR+ GS D++  + R P    NF+  + G  L DLVS++           +  
Sbjct: 508 GMKGSFATRVSGSSDLYQVNQRKPYHGVNFVIAHDGFTLRDLVSYNFKHNEANGEGGNDG 567

Query: 717 LASELSWNCGEEGPTSNIEVLEKRLKLIRNFIFVLFVSLGVPVLNMGDECGQSS-GGSIA 776
                SWNCG EG T +  +   R + ++NF   L +S G P++ MGDE G +  G + +
Sbjct: 568 CNDNHSWNCGFEGETGDAHIKSLRTRQMKNFHLALMISQGTPMMLMGDEYGHTRYGNNNS 627

Query: 777 F---KDRRPFDWDLLKTGFGTQTTQFITFFSSFRARRFD--LFQSRNFLKGENIDWYGNN 836
           +        F W  L      +      FFS     R    + +  NFL    I W+ +N
Sbjct: 628 YGHDTSLNNFQWKEL----DAKKQNHFRFFSEVIKFRHSHHVLKHENFLTQGEITWHEDN 687

Query: 837 LSPPQWEDPSCKFLAVMLRADKEENGLSAENPEPQSDIFMAFNASDQSINVALPEPSEGT 888
                W++   KFLA  L      +G+         DI++AFNA D  +   +P+P  G 
Sbjct: 688 -----WDNSESKFLAFTL-----HDGIGGR------DIYVAFNAHDYFVKALIPQPPPGK 747

BLAST of Moc09g07920 vs. ExPASy Swiss-Prot
Match: B9G434 (Isoamylase 3, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=ISA3 PE=2 SV=1)

HSP 1 Score: 369.0 bits (946), Expect = 1.5e-100
Identity = 254/714 (35.57%), Postives = 366/714 (51.26%), Query Frame = 0

Query: 230 VPVGFGRGYPSPLGVSISGDGSMNFSIFSSSAESVVLCLY-----NDSTSEKPVIELDLD 289
           +P  +  G   PLGVS   +G +NF++FS  A SV+LCL       +      V+E  LD
Sbjct: 96  MPFKYSSGKAFPLGVS-QVEGGLNFALFSQHASSVILCLKLPGRGTEDEKGADVVEFVLD 155

Query: 290 RYINRSGNIWHVSFESV-SNFVSYGYLCKGSKSHKKQDGVEISRILVDPYAKVLTTSSHK 349
           +  N++G+IWHV  E + ++ V YGY   G +   +    + S +L+DPYAK++  S  K
Sbjct: 156 QQKNKTGDIWHVIVEGLPASGVLYGYRVGGPQGWDQGHRFDSSTVLLDPYAKLV--SGRK 215

Query: 350 THGHGLGLPSKLLGQI-CKVPAFDWDGEVR-PNLAMEKLFTYRLNVKRFTVDKSSQLTTD 409
             G      S+  G        FDW  + R PNL    L  Y +NV+ FT D+SS L + 
Sbjct: 216 YFGVAEEKSSQHFGTYDFDSSPFDWGDDYRLPNLPEADLVIYEMNVRAFTADESSGLDST 275

Query: 410 IAGTFAGLTKKLLHFKNLGVNAVLLEPIFPFDEKEGPYFPS--------------HFFSP 469
             G++ GL  K+ H   LGVNAV L P+F +DE E   +P+              +FF+P
Sbjct: 276 SRGSYLGLIDKIPHLLELGVNAVELLPVFEYDELEFKRYPNPRDHMVNTWGYSTINFFAP 335

Query: 470 TNIYGPSGGS-ISAINSMKEMVKELHANGIEVMLEVVF--TNTAEDG-----TLQGIDDS 529
            + Y  +GG  ++A   +K+MVKELH  GIEV+L+VV+  TN A+D      + +GID+ 
Sbjct: 336 MSRYASAGGGPVAASKELKQMVKELHKAGIEVILDVVYNHTNEADDAHPYMTSFRGIDNK 395

Query: 530 SYYFTNRVANLE------GKNALNCNYPVVQQLLLDSLRYWVTEFHIDGFCFINASSLLR 589
            YY  +   N E        N LNCN+PVV++L+LDSLR+WV E+HIDGF F  AS L R
Sbjct: 396 VYYMLDLNKNAELLNFSGCGNTLNCNHPVVKELILDSLRHWVEEYHIDGFRFDLASVLCR 455

Query: 590 GYRGELLSRPPLVEAIAFDPLLSKTKIIADFWDPHESISKETRFPHWKRWAEVNYKFCSD 649
           G  G  L  PPL++ IA D +LS+ KIIA+ WD    +    RFP+W RWAE N K+  D
Sbjct: 456 GPDGCPLDAPPLIKEIAKDAVLSRCKIIAEPWD-CGGLYLVGRFPNWDRWAEWNGKYRDD 515

Query: 650 VRDFFRGE-GLVSSLATRLCGSGDIFS-DGRGPAFSFNFIARNFGLPLVDLVSFS----- 709
           +R F +G+ G+    ATR+ GS D++  + R P    NF+  + G  L DLVS++     
Sbjct: 516 LRRFIKGDPGMKGVFATRVSGSADLYQVNERKPYHGVNFVIAHDGFTLCDLVSYNLKHND 575

Query: 710 ------SSKLASELSWNCGEEGPTSNIEVLEKRLKLIRNFIFVLFVSLGVPVLNMGDECG 769
                         SWNCG EG T+++ VL  R + ++NF   L +S G P++ MGDE G
Sbjct: 576 ANGEGGCDGCNDNFSWNCGVEGETNDLNVLSLRSRQMKNFHVALMISQGTPMMLMGDEYG 635

Query: 770 QSSGGSIAFKDR----RPFDWDLL---KTGFGTQTTQFITFFSSFRARRFDLFQSRNFLK 829
            +  G+            F W+ L   + G     ++ I F  S    R D F ++N   
Sbjct: 636 HTRYGNNNSYGHDTCINNFQWEQLEQRRDGHFRFFSEMIKFRHSNPILRRDRFLNKN--- 695

Query: 830 GENIDWYGNNLSPPQWEDPSCKFLAVMLRADKEENGLSAENPEPQSDIFMAFNASDQSIN 888
             ++ W+ +      WE+   KFLA  +  D    G          DI++AFNA D  ++
Sbjct: 696 --DVTWHED-----CWENQESKFLAFTVH-DHNSGG----------DIYLAFNAHDYFVD 755

BLAST of Moc09g07920 vs. ExPASy Swiss-Prot
Match: D0TZF0 (Isoamylase 1, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=ISA1 PE=1 SV=1)

HSP 1 Score: 341.3 bits (874), Expect = 3.4e-92
Identity = 233/690 (33.77%), Postives = 342/690 (49.57%), Query Frame = 0

Query: 237 GYPSPLGVSISGDGSMNFSIFSSSAESVVLCLY--NDSTSEKPVIELDLDRYINRSGNIW 296
           G P+PLG + + DG +NF+++S+ A +  LCL+  +D  +++   E+ LD   NR+GN+W
Sbjct: 99  GMPAPLGAT-ALDGGVNFAVYSAGASAASLCLFTPDDLEADEVTEEVPLDPLFNRTGNVW 158

Query: 297 HVSFESVSNFVSYGYLCKGSKSHKKQDGVEISRILVDPYAK-VLTTSSHKTHGHGLGLPS 356
           HV  E   + + YGY   G  +       ++S ++VDPYAK V++   +   G G     
Sbjct: 159 HVFIEGELHNMLYGYRFDGMFAPHCGQYFDVSNVVVDPYAKAVISRGEYGVPGPGGDCWP 218

Query: 357 KLLGQI-CKVPAFDWDGEVRPNLAMEKLFTYRLNVKRFTVDKSSQLTTDIAGTFAGLTKK 416
           ++ G I      FDW G++      + L  Y ++++ FT  K S    +  GT+ G   K
Sbjct: 219 QMAGMIPLPYSTFDWQGDLPLRYPQKDLVIYEMHLRGFT--KHSSSNVEHPGTYIGAISK 278

Query: 417 LLHFKNLGVNAVLLEPIFPFDEKE----------GPYFPSHFFSPTNIYGPSGG----SI 476
           L + K LGVN V L P   F+E E            Y   +FFSP   Y  SGG      
Sbjct: 279 LDYLKELGVNCVELMPCHEFNELEYFSCSSKMNFWGYSTINFFSPMIRYS-SGGIRNCGR 338

Query: 477 SAINSMKEMVKELHANGIEVMLEVVFTNTAEDG------TLQGIDDSSYYF---TNRVAN 536
            AIN  K  V+E H  GIEV+++VVF +TAE        + +GID+S+YY         N
Sbjct: 339 DAINEFKTFVREAHKRGIEVIMDVVFNHTAEGNEKGPILSFRGIDNSTYYMLAPKGEFYN 398

Query: 537 LEG-KNALNCNYPVVQQLLLDSLRYWVTEFHIDGFCFINASSLLRG-------------- 596
             G  N  NCN+PVV++ ++D LRYWVTE H+DGF F  AS + RG              
Sbjct: 399 YSGCGNTFNCNHPVVREFIVDCLRYWVTEMHVDGFRFDLASIMTRGCSLWDPVNVYGSPV 458

Query: 597 -----YRGELLSRPPLVEAIAFDPLLSKTKIIADFWDPHESISKETRFPHWKRWAEVNYK 656
                  G  L+ PPL++ I+ DP+L   K+IA+ WD    + +  +FPHWK W+E N K
Sbjct: 459 EGDMTTTGTPLATPPLIDMISNDPILGDVKLIAEAWDA-GGLYQVGQFPHWKIWSEWNGK 518

Query: 657 FCSDVRDFFRG-EGLVSSLATRLCGSGDIF-SDGRGPAFSFNFIARNFGLPLVDLVSFSS 716
           +   VR F +G +G     A  LCGS  ++ + GR P  S NF+  + G  L DLV+++ 
Sbjct: 519 YRDIVRQFIKGTDGFAGGFAECLCGSPHLYQAGGRKPWHSINFVCAHDGFTLADLVTYNK 578

Query: 717 SKLAS-----------ELSWNCGEEGPTSNIEVLEKRLKLIRNFIFVLFVSLGVPVLNMG 776
              +S            LSWNCGEEG  + + V   R + +RNF   L VS GVP+  MG
Sbjct: 579 KYNSSNGEDNRDGENHNLSWNCGEEGEFAGLSVKRLRKRQMRNFFVSLMVSQGVPMFYMG 638

Query: 777 DECGQSSGG--SIAFKDR--RPFDWDLLKTGFGTQTTQFITFFSSFRARRFDLFQSRNFL 836
           DE G + GG  +    D     F WD  K    +   +F +  + FR ++ +     +F 
Sbjct: 639 DEYGHTKGGNNNTYCHDHYVNYFRWD--KKEESSDLQRFCSLMTKFR-KQCESLGLADFP 698

Query: 837 KGENIDWYGNNLSPPQWEDPSCKFLAVMLRADKEENGLSAENPEPQSDIFMAFNASDQSI 863
             + + W+G+    P W + S +F+A             +   E + +I++AFNAS    
Sbjct: 699 TAQRLHWHGHQPGKPDWSETS-RFVAF------------STKDETKGEIYVAFNASHLPA 758

BLAST of Moc09g07920 vs. ExPASy TrEMBL
Match: A0A6J1DAX4 (isoamylase 2, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111018448 PE=4 SV=1)

HSP 1 Score: 1814.3 bits (4698), Expect = 0.0e+00
Identity = 891/891 (100.00%), Postives = 891/891 (100.00%), Query Frame = 0

Query: 1   MLLQPQMATFPSSLVVQPYNVHNFGVTEAFRLAASDHHTHGHMIKHQSGKRDEVRMLAHG 60
           MLLQPQMATFPSSLVVQPYNVHNFGVTEAFRLAASDHHTHGHMIKHQSGKRDEVRMLAHG
Sbjct: 1   MLLQPQMATFPSSLVVQPYNVHNFGVTEAFRLAASDHHTHGHMIKHQSGKRDEVRMLAHG 60

Query: 61  ESRAGFAKSSRYNLSKAYASFGISVEKNGWRFGISGKSKEQEKVATYLFRTECGDLVNVL 120
           ESRAGFAKSSRYNLSKAYASFGISVEKNGWRFGISGKSKEQEKVATYLFRTECGDLVNVL
Sbjct: 61  ESRAGFAKSSRYNLSKAYASFGISVEKNGWRFGISGKSKEQEKVATYLFRTECGDLVNVL 120

Query: 121 VRKTGSGFMVSIELSSVQLANIEETPMLSWGVYRSDSALVTPNIQSSLPDDTIGTIETPF 180
           VRKTGSGFMVSIELSSVQLANIEETPMLSWGVYRSDSALVTPNIQSSLPDDTIGTIETPF
Sbjct: 121 VRKTGSGFMVSIELSSVQLANIEETPMLSWGVYRSDSALVTPNIQSSLPDDTIGTIETPF 180

Query: 181 VNTSEGKFAVELEFDAKHVPFYLSFLLKHPVGVDSGSSEIRSHRKTSFCVPVGFGRGYPS 240
           VNTSEGKFAVELEFDAKHVPFYLSFLLKHPVGVDSGSSEIRSHRKTSFCVPVGFGRGYPS
Sbjct: 181 VNTSEGKFAVELEFDAKHVPFYLSFLLKHPVGVDSGSSEIRSHRKTSFCVPVGFGRGYPS 240

Query: 241 PLGVSISGDGSMNFSIFSSSAESVVLCLYNDSTSEKPVIELDLDRYINRSGNIWHVSFES 300
           PLGVSISGDGSMNFSIFSSSAESVVLCLYNDSTSEKPVIELDLDRYINRSGNIWHVSFES
Sbjct: 241 PLGVSISGDGSMNFSIFSSSAESVVLCLYNDSTSEKPVIELDLDRYINRSGNIWHVSFES 300

Query: 301 VSNFVSYGYLCKGSKSHKKQDGVEISRILVDPYAKVLTTSSHKTHGHGLGLPSKLLGQIC 360
           VSNFVSYGYLCKGSKSHKKQDGVEISRILVDPYAKVLTTSSHKTHGHGLGLPSKLLGQIC
Sbjct: 301 VSNFVSYGYLCKGSKSHKKQDGVEISRILVDPYAKVLTTSSHKTHGHGLGLPSKLLGQIC 360

Query: 361 KVPAFDWDGEVRPNLAMEKLFTYRLNVKRFTVDKSSQLTTDIAGTFAGLTKKLLHFKNLG 420
           KVPAFDWDGEVRPNLAMEKLFTYRLNVKRFTVDKSSQLTTDIAGTFAGLTKKLLHFKNLG
Sbjct: 361 KVPAFDWDGEVRPNLAMEKLFTYRLNVKRFTVDKSSQLTTDIAGTFAGLTKKLLHFKNLG 420

Query: 421 VNAVLLEPIFPFDEKEGPYFPSHFFSPTNIYGPSGGSISAINSMKEMVKELHANGIEVML 480
           VNAVLLEPIFPFDEKEGPYFPSHFFSPTNIYGPSGGSISAINSMKEMVKELHANGIEVML
Sbjct: 421 VNAVLLEPIFPFDEKEGPYFPSHFFSPTNIYGPSGGSISAINSMKEMVKELHANGIEVML 480

Query: 481 EVVFTNTAEDGTLQGIDDSSYYFTNRVANLEGKNALNCNYPVVQQLLLDSLRYWVTEFHI 540
           EVVFTNTAEDGTLQGIDDSSYYFTNRVANLEGKNALNCNYPVVQQLLLDSLRYWVTEFHI
Sbjct: 481 EVVFTNTAEDGTLQGIDDSSYYFTNRVANLEGKNALNCNYPVVQQLLLDSLRYWVTEFHI 540

Query: 541 DGFCFINASSLLRGYRGELLSRPPLVEAIAFDPLLSKTKIIADFWDPHESISKETRFPHW 600
           DGFCFINASSLLRGYRGELLSRPPLVEAIAFDPLLSKTKIIADFWDPHESISKETRFPHW
Sbjct: 541 DGFCFINASSLLRGYRGELLSRPPLVEAIAFDPLLSKTKIIADFWDPHESISKETRFPHW 600

Query: 601 KRWAEVNYKFCSDVRDFFRGEGLVSSLATRLCGSGDIFSDGRGPAFSFNFIARNFGLPLV 660
           KRWAEVNYKFCSDVRDFFRGEGLVSSLATRLCGSGDIFSDGRGPAFSFNFIARNFGLPLV
Sbjct: 601 KRWAEVNYKFCSDVRDFFRGEGLVSSLATRLCGSGDIFSDGRGPAFSFNFIARNFGLPLV 660

Query: 661 DLVSFSSSKLASELSWNCGEEGPTSNIEVLEKRLKLIRNFIFVLFVSLGVPVLNMGDECG 720
           DLVSFSSSKLASELSWNCGEEGPTSNIEVLEKRLKLIRNFIFVLFVSLGVPVLNMGDECG
Sbjct: 661 DLVSFSSSKLASELSWNCGEEGPTSNIEVLEKRLKLIRNFIFVLFVSLGVPVLNMGDECG 720

Query: 721 QSSGGSIAFKDRRPFDWDLLKTGFGTQTTQFITFFSSFRARRFDLFQSRNFLKGENIDWY 780
           QSSGGSIAFKDRRPFDWDLLKTGFGTQTTQFITFFSSFRARRFDLFQSRNFLKGENIDWY
Sbjct: 721 QSSGGSIAFKDRRPFDWDLLKTGFGTQTTQFITFFSSFRARRFDLFQSRNFLKGENIDWY 780

Query: 781 GNNLSPPQWEDPSCKFLAVMLRADKEENGLSAENPEPQSDIFMAFNASDQSINVALPEPS 840
           GNNLSPPQWEDPSCKFLAVMLRADKEENGLSAENPEPQSDIFMAFNASDQSINVALPEPS
Sbjct: 781 GNNLSPPQWEDPSCKFLAVMLRADKEENGLSAENPEPQSDIFMAFNASDQSINVALPEPS 840

Query: 841 EGTSWFRLIDTALSFPGFFSSDGELVPTVESVTYEMQAHSCALFEAKSYSD 892
           EGTSWFRLIDTALSFPGFFSSDGELVPTVESVTYEMQAHSCALFEAKSYSD
Sbjct: 841 EGTSWFRLIDTALSFPGFFSSDGELVPTVESVTYEMQAHSCALFEAKSYSD 891

BLAST of Moc09g07920 vs. ExPASy TrEMBL
Match: A0A0A0K551 (Aamy domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G392380 PE=4 SV=1)

HSP 1 Score: 1469.1 bits (3802), Expect = 0.0e+00
Identity = 713/885 (80.56%), Postives = 784/885 (88.59%), Query Frame = 0

Query: 7   MATFPSSLVVQPYNVHNFGVTEAFRLAASDHHTHGHMIKHQSGKRDEVRMLAHGESRAGF 66
           MATF SSLV+QP+ V+N GVTE+ +LAASDH T+G   K+Q GK DE RMLAHGE++ G 
Sbjct: 1   MATFLSSLVIQPFCVYNCGVTESPKLAASDHLTYGQKTKYQFGKMDEARMLAHGENKVGA 60

Query: 67  AKSSRYNLSKAYASFGISVEKNGWRFGISGKSKEQEKVATYLFRTECGDLVNVLVRKTGS 126
            KSS  NLSK YA  GISV K+G R GI GKSKEQ +VATYLFRTE GDLVNV V K GS
Sbjct: 61  VKSSHRNLSKTYAKSGISVGKSGQRLGIGGKSKEQRRVATYLFRTEFGDLVNVFVGKKGS 120

Query: 127 GFMVSIELSSVQLANIEETPMLSWGVYRSDSALVTPNIQSSLPDDTIGTIETPFVNTSEG 186
            F V+IE+ S+QL +I+E  +LSWGVYRSDSALVTPN +SS PD+T G  ETPFV TSEG
Sbjct: 121 TFTVNIEVPSMQLVSIDEALLLSWGVYRSDSALVTPNFESSPPDETTGATETPFVKTSEG 180

Query: 187 KFAVELEFDAKHVPFYLSFLLKHPVGVDSGSSEIRSHRKTSFCVPVGFGRGYPSPLGVSI 246
           KF+VELEFDAKH PFYLSF+LK+P+GVDSGSSEIRSH+KTSF VPVGFGRGYPSPLG+SI
Sbjct: 181 KFSVELEFDAKHTPFYLSFVLKYPMGVDSGSSEIRSHKKTSFSVPVGFGRGYPSPLGLSI 240

Query: 247 SGDGSMNFSIFSSSAESVVLCLYNDSTSEKPVIELDLDRYINRSGNIWHVSFESVSNFVS 306
           SGDGS+NFSIFSSSAES+VLCLYNDSTSEKP++ELDLD YINRSGNIWH SFE  S FVS
Sbjct: 241 SGDGSVNFSIFSSSAESLVLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGASKFVS 300

Query: 307 YGYLCKGSKSHKKQDGVEISRILVDPYAKVLTTSSHKTHGHGLGLPSKLLGQICKVPAFD 366
           YGY CKGSKSH+ QDG+E+SRI+VDPYAK+L  S  K+ G GLGLPSK LGQI KVP FD
Sbjct: 301 YGYQCKGSKSHENQDGLEVSRIVVDPYAKILAPSIPKSSGQGLGLPSKFLGQISKVPTFD 360

Query: 367 WDGEVRPNLAMEKLFTYRLNVKRFTVDKSSQLTTDIAGTFAGLTKKLLHFKNLGVNAVLL 426
           WDGEV PNL MEKLF YRLNV+RFT+DKSSQL  DIAGTF+GLTKKLLHFKNLGVNAVLL
Sbjct: 361 WDGEVHPNLPMEKLFVYRLNVERFTMDKSSQLPADIAGTFSGLTKKLLHFKNLGVNAVLL 420

Query: 427 EPIFPFDEKEGPYFPSHFFSPTNIYGPSGGSISAINSMKEMVKELHANGIEVMLEVVFTN 486
           EPIF FDEKEGPYFP HFFSPTN YGPSG SISAINSMKEMVKELHANG+EV+LEVV+T+
Sbjct: 421 EPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVILEVVYTH 480

Query: 487 TAEDGTLQGIDDSSYYFTNRVANLEGKNALNCNYPVVQQLLLDSLRYWVTEFHIDGFCFI 546
           T+ +G LQGIDDSSYYFTNRVANLE K+ALNCNYP+VQQLLLDSLRYWVTEFH+DGFCF+
Sbjct: 481 TSGNGALQGIDDSSYYFTNRVANLEEKSALNCNYPIVQQLLLDSLRYWVTEFHVDGFCFV 540

Query: 547 NASSLLRGYRGELLSRPPLVEAIAFDPLLSKTKIIADFWDPHESISKETRFPHWKRWAEV 606
           NAS LLRG+ GELLSRPP VEAIAFDPLLSKTK++ADFWDP E  SKETRFPHWKRWAEV
Sbjct: 541 NASFLLRGHHGELLSRPPFVEAIAFDPLLSKTKLVADFWDPQELESKETRFPHWKRWAEV 600

Query: 607 NYKFCSDVRDFFRGEGLVSSLATRLCGSGDIFSDGRGPAFSFNFIARNFGLPLVDLVSFS 666
           N KFCSD+RDFFRGEGL+SSLATRLCGSGD+FSDGRGPAFSFNFIARN GLPLVDLVSFS
Sbjct: 601 NSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS 660

Query: 667 SSKLASELSWNCGEEGPTSNIEVLEKRLKLIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 726
           +S LASELSWNCGEEGPTSN++VLEKRLK IRNFIFVLFVSLGVPVLNMGDECGQSSGGS
Sbjct: 661 NSNLASELSWNCGEEGPTSNLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 720

Query: 727 IAFKDRRPFDWDLLKTGFGTQTTQFITFFSSFRARRFDLFQSRNFLKGENIDWYGNNLSP 786
           +AF D+R F+WDLLKT FGTQTTQFI F SSFR+RRFDLFQ+RNFLKGENIDW+ NN SP
Sbjct: 721 VAFNDKRSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLFQNRNFLKGENIDWFDNNQSP 780

Query: 787 PQWEDPSCKFLAVMLRADKEENGLSAENPEPQSDIFMAFNASDQSINVALPEPSEGTSWF 846
           PQWED SCKFLAVMLRADKEEN    ENP+ +S+IFM FNASDQS +VALPEP EGTSWF
Sbjct: 781 PQWEDASCKFLAVMLRADKEENESITENPKTRSNIFMVFNASDQSESVALPEPLEGTSWF 840

Query: 847 RLIDTALSFPGFFSSDGELVPTVESVTYEMQAHSCALFEAKSYSD 892
           R++DTAL FPGFFSSDGELVP   SVTYE+QAHSCALFEAKS +D
Sbjct: 841 RVVDTALPFPGFFSSDGELVPMTGSVTYEIQAHSCALFEAKSAND 885

BLAST of Moc09g07920 vs. ExPASy TrEMBL
Match: A0A5A7UR47 (Isoamylase 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold328G00230 PE=4 SV=1)

HSP 1 Score: 1468.4 bits (3800), Expect = 0.0e+00
Identity = 714/882 (80.95%), Postives = 778/882 (88.21%), Query Frame = 0

Query: 7   MATFPSSLVVQPYNVHNFGVTEAFRLAASDHHTHGHMIKHQSGKRDEVRMLAHGESRAGF 66
           MATF SSLV+QP+ VHN GVTE+ +LA SDH T+G   K+QSGK DE RMLAHGES+ G 
Sbjct: 1   MATFLSSLVIQPFCVHNCGVTESLKLAVSDHLTYGQKTKYQSGKMDEARMLAHGESKVGA 60

Query: 67  AKSSRYNLSKAYASFGISVEKNGWRFGISGKSKEQEKVATYLFRTECGDLVNVLVRKTGS 126
            KSS  NLSKAY + GISV K+G R GI GKSKEQ KVATYLFRTE GDLVNV V KTGS
Sbjct: 61  VKSSHRNLSKAYVTSGISVGKSGQRLGIGGKSKEQRKVATYLFRTEFGDLVNVFVGKTGS 120

Query: 127 GFMVSIELSSVQLANIEETPMLSWGVYRSDSALVTPNIQSSLPDDTIGTIETPFVNTSEG 186
            F V+IE+SS+QL + +ET +LSW VYRSDSA VT N QSS PD T G  ETPFV TSEG
Sbjct: 121 AFTVNIEISSMQLVSTDETLLLSWRVYRSDSAPVTSNFQSSPPDGTTGATETPFVKTSEG 180

Query: 187 KFAVELEFDAKHVPFYLSFLLKHPVGVDSGSSEIRSHRKTSFCVPVGFGRGYPSPLGVSI 246
           KF+VELEFDAKH PFYLSF+LK P+GVDSGSSEIRSHRKTSF VPVGFGRGYPSP G+SI
Sbjct: 181 KFSVELEFDAKHTPFYLSFVLKFPMGVDSGSSEIRSHRKTSFSVPVGFGRGYPSPSGLSI 240

Query: 247 SGDGSMNFSIFSSSAESVVLCLYNDSTSEKPVIELDLDRYINRSGNIWHVSFESVSNFVS 306
           SGDGS+NFSIFSS+AESVVLCLYNDSTSEKP++ELDLD YINRSGNIWH SFE VSNFVS
Sbjct: 241 SGDGSINFSIFSSTAESVVLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGVSNFVS 300

Query: 307 YGYLCKGSKSHKKQDGVEISRILVDPYAKVLTTSSHKTHGHGLGLPSKLLGQICKVPAFD 366
           YGY CKG KSH+ QDG+E+SRI+VDPYAK+L  S  K+   GLG P K LGQI  VPAFD
Sbjct: 301 YGYQCKGLKSHENQDGLEVSRIVVDPYAKILAPSIPKSRDQGLGFPLKFLGQISIVPAFD 360

Query: 367 WDGEVRPNLAMEKLFTYRLNVKRFTVDKSSQLTTDIAGTFAGLTKKLLHFKNLGVNAVLL 426
           WDGEVRPNL MEKLF YRLNVKRFT+DKSSQL  DIAGTF+GLTKKLLH KNLGVNAVLL
Sbjct: 361 WDGEVRPNLPMEKLFVYRLNVKRFTIDKSSQLPADIAGTFSGLTKKLLHLKNLGVNAVLL 420

Query: 427 EPIFPFDEKEGPYFPSHFFSPTNIYGPSGGSISAINSMKEMVKELHANGIEVMLEVVFTN 486
           EPIF FDEKEGPYFP HFFSPTN YGPSG SISAINSMKEMVKELHANG+EV++EVV+T+
Sbjct: 421 EPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVIVEVVYTH 480

Query: 487 TAEDGTLQGIDDSSYYFTNRVANLEGKNALNCNYPVVQQLLLDSLRYWVTEFHIDGFCFI 546
           T+ DG LQGIDDSSYYF NRVANLE ++ALNCNYP+VQQLLLDSLRYWVTEFH+DGFCF+
Sbjct: 481 TSVDGALQGIDDSSYYFANRVANLEERSALNCNYPIVQQLLLDSLRYWVTEFHVDGFCFV 540

Query: 547 NASSLLRGYRGELLSRPPLVEAIAFDPLLSKTKIIADFWDPHESISKETRFPHWKRWAEV 606
           NAS LL+G+ GELLSRPP VEAIAFDP+LSKTK++ADFWDP E +SKETRFPHWKRWAEV
Sbjct: 541 NASFLLQGHHGELLSRPPFVEAIAFDPILSKTKLVADFWDPQELVSKETRFPHWKRWAEV 600

Query: 607 NYKFCSDVRDFFRGEGLVSSLATRLCGSGDIFSDGRGPAFSFNFIARNFGLPLVDLVSFS 666
           N KFCSD+RDFFRGEGL+SSLATRLCGSGD+FSDGRGPAFSFNFIARN GLPLVDLVSFS
Sbjct: 601 NSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS 660

Query: 667 SSKLASELSWNCGEEGPTSNIEVLEKRLKLIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 726
           +S LASELSWNCGEEGPT+ ++VLEKRLK IRNFIFVLFVSLGVPVLNMGDECGQSSGGS
Sbjct: 661 NSNLASELSWNCGEEGPTNTLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 720

Query: 727 IAFKDRRPFDWDLLKTGFGTQTTQFITFFSSFRARRFDLFQSRNFLKGENIDWYGNNLSP 786
           +AF DRR F+WDLLKT FGTQTTQFI F SSFR+RRFDLFQSRNFLKGENIDW+ NN SP
Sbjct: 721 VAFNDRRSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLFQSRNFLKGENIDWFDNNQSP 780

Query: 787 PQWEDPSCKFLAVMLRADKEENGLSAENPEPQSDIFMAFNASDQSINVALPEPSEGTSWF 846
           PQWED SCKFLAVMLRADKEEN    ENP+ +SDIFMAFNASDQS +VALPEPSEGTSWF
Sbjct: 781 PQWEDASCKFLAVMLRADKEENESITENPKTRSDIFMAFNASDQSESVALPEPSEGTSWF 840

Query: 847 RLIDTALSFPGFFSSDGELVPTVESVTYEMQAHSCALFEAKS 889
           R++DTAL FPGFFSSDGELVP   SVTYEMQAHSCALFEAKS
Sbjct: 841 RVVDTALPFPGFFSSDGELVPMTGSVTYEMQAHSCALFEAKS 882

BLAST of Moc09g07920 vs. ExPASy TrEMBL
Match: A0A6J1GPU3 (isoamylase 2, chloroplastic isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111456039 PE=4 SV=1)

HSP 1 Score: 1467.6 bits (3798), Expect = 0.0e+00
Identity = 714/885 (80.68%), Postives = 785/885 (88.70%), Query Frame = 0

Query: 7   MATFPSSLVVQPYNVHNFGVTEAFRLAASDHHTHGHMIKHQSGKRDEVRMLAHGESRAGF 66
           MATF SSL +QP+  +N G TE+F+LA SD+HT+G  + +QSGK DE RM AHGES+ GF
Sbjct: 1   MATFLSSL-IQPFCGYNRGGTESFKLAVSDYHTYGQKMNYQSGKLDEARMQAHGESKVGF 60

Query: 67  AKSSRYNLSKAYASFGISVEKNGWRFGISGKSKEQEKVATYLFRTECGDLVNVLVRKTGS 126
            KS   N+ +AYA   ISV K+G +FG  GK++E  KVATYLFRTE GDLV   V K GS
Sbjct: 61  TKSLHRNVCRAYAPSRISVGKSGQKFGTGGKTEEDRKVATYLFRTEFGDLVYAFVGKRGS 120

Query: 127 GFMVSIELSSVQLANIEETPMLSWGVYRSDSALVTPNIQSSLPDDTIGTIETPFVNTSEG 186
           GFM SIE+SS++LA+I+ET +LSWGVYRS+SA+VTPN QS LP++TIG  ETPFV TSEG
Sbjct: 121 GFMFSIEISSLKLASIDETLLLSWGVYRSNSAIVTPNFQSILPEETIGATETPFVRTSEG 180

Query: 187 KFAVELEFDAKHVPFYLSFLLKHPVGVDSGSSEIRSHRKTSFCVPVGFGRGYPSPLGVSI 246
           KFAVELEFDAKH PFYLSFLLK+PVGVDSGSSEI+SHRKTSF VPVGFGRGYPSPLG+SI
Sbjct: 181 KFAVELEFDAKHDPFYLSFLLKYPVGVDSGSSEIKSHRKTSFSVPVGFGRGYPSPLGLSI 240

Query: 247 SGDGSMNFSIFSSSAESVVLCLYNDSTSEKPVIELDLDRYINRSGNIWHVSFESVSNFVS 306
           SGDGS+NFSIFSS+AESVVLCLYNDSTSEKPV+ELDLD YINRSGNIWH SFE  SNFVS
Sbjct: 241 SGDGSINFSIFSSTAESVVLCLYNDSTSEKPVLELDLDPYINRSGNIWHASFEGASNFVS 300

Query: 307 YGYLCKGSKSHKKQDGVEISRILVDPYAKVLTTSSHKTHGHGLGLPSKLLGQICKVPAFD 366
           YGY C+GSK H+ QD  E+SRILVDPYAK+LT S  KT G GLGLPSK LGQIC VPAF 
Sbjct: 301 YGYRCRGSKFHENQDEAEVSRILVDPYAKILTASIPKTRGQGLGLPSKFLGQICNVPAFS 360

Query: 367 WDGEVRPNLAMEKLFTYRLNVKRFTVDKSSQLTTDIAGTFAGLTKKLLHFKNLGVNAVLL 426
           WDGEVRPNL MEKLF YRLNVK FTVDKSSQL  D AGTF+GLT+KLLHFK LGVNAVLL
Sbjct: 361 WDGEVRPNLPMEKLFIYRLNVKHFTVDKSSQLPIDTAGTFSGLTQKLLHFKKLGVNAVLL 420

Query: 427 EPIFPFDEKEGPYFPSHFFSPTNIYGPSGGSISAINSMKEMVKELHANGIEVMLEVVFTN 486
           EPIFPFDEK+GPYFP HFFSPTNIY PSG SIS++NS+KEMVKELHANG+EV+LEVVFT+
Sbjct: 421 EPIFPFDEKDGPYFPFHFFSPTNIYVPSGASISSLNSVKEMVKELHANGVEVILEVVFTH 480

Query: 487 TAEDGTLQGIDDSSYYFTNRVANLEGKNALNCNYPVVQQLLLDSLRYWVTEFHIDGFCFI 546
           TA DG LQGIDDS YYF NRVAN EGK+ALNCN+P+VQQLLLDSLR+WVTEFHIDGFCFI
Sbjct: 481 TAMDGALQGIDDSCYYFVNRVANSEGKSALNCNHPIVQQLLLDSLRHWVTEFHIDGFCFI 540

Query: 547 NASSLLRGYRGELLSRPPLVEAIAFDPLLSKTKIIADFWDPHESISKETRFPHWKRWAEV 606
           NASSLL+G+ GELLSRPP VEAIAFDPLLSKTKIIADFWDP ES+ KETRFPHWKRWAE+
Sbjct: 541 NASSLLQGHHGELLSRPPFVEAIAFDPLLSKTKIIADFWDPQESVPKETRFPHWKRWAEL 600

Query: 607 NYKFCSDVRDFFRGEGLVSSLATRLCGSGDIFSDGRGPAFSFNFIARNFGLPLVDLVSFS 666
           N KF +D+++FFRGEGLVSSLATRLCGSGD+FSDGRGPAFSFNFIARN GLPLVDLVSFS
Sbjct: 601 NSKFSNDIKEFFRGEGLVSSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS 660

Query: 667 SSKLASELSWNCGEEGPTSNIEVLEKRLKLIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 726
           SSKLASELSWNCGEEGPTSNIEVLEKRLK IRNFIFVLFVSLGVPVLNMGDECGQSSGGS
Sbjct: 661 SSKLASELSWNCGEEGPTSNIEVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 720

Query: 727 IAFKDRRPFDWDLLKTGFGTQTTQFITFFSSFRARRFDLFQSRNFLKGENIDWYGNNLSP 786
           +AFKDR+ F+WD LKT FGTQTTQFITF SSFR+RRFDLFQSRNFLK ENIDW+ NN SP
Sbjct: 721 VAFKDRKSFNWDSLKTDFGTQTTQFITFLSSFRSRRFDLFQSRNFLKVENIDWFDNNQSP 780

Query: 787 PQWEDPSCKFLAVMLRADKEENGLSAENPEPQSDIFMAFNASDQSINVALPEPSEGTSWF 846
           P WEDPSCKFLAVMLRADKEE+G   ENP+PQSDIFMAFNASDQS++VALPEPSEGT+WF
Sbjct: 781 PDWEDPSCKFLAVMLRADKEESGSITENPKPQSDIFMAFNASDQSVSVALPEPSEGTNWF 840

Query: 847 RLIDTALSFPGFFSSDGELVPTVESVTYEMQAHSCALFEAKSYSD 892
           RL+DTALSFPGFFSSDGE VPT   VTY MQAHSC LFEAKS++D
Sbjct: 841 RLVDTALSFPGFFSSDGEPVPTTSPVTYGMQAHSCTLFEAKSFND 884

BLAST of Moc09g07920 vs. ExPASy TrEMBL
Match: A0A1S3BRF8 (isoamylase 2, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103492696 PE=4 SV=1)

HSP 1 Score: 1466.8 bits (3796), Expect = 0.0e+00
Identity = 713/882 (80.84%), Postives = 777/882 (88.10%), Query Frame = 0

Query: 7   MATFPSSLVVQPYNVHNFGVTEAFRLAASDHHTHGHMIKHQSGKRDEVRMLAHGESRAGF 66
           MATF SSLV+QP+ VHN GVTE+ +LA SDH T+G   K+QSGK DE RMLAHGES+ G 
Sbjct: 1   MATFLSSLVIQPFCVHNCGVTESLKLAVSDHLTYGQKTKYQSGKMDEARMLAHGESKVGA 60

Query: 67  AKSSRYNLSKAYASFGISVEKNGWRFGISGKSKEQEKVATYLFRTECGDLVNVLVRKTGS 126
            KSS  NLSKAY + GI V K+G R GI GKSKEQ KVATYLFRTE GDLVNV V KTGS
Sbjct: 61  VKSSHRNLSKAYVTSGILVGKSGQRLGIGGKSKEQRKVATYLFRTEFGDLVNVFVGKTGS 120

Query: 127 GFMVSIELSSVQLANIEETPMLSWGVYRSDSALVTPNIQSSLPDDTIGTIETPFVNTSEG 186
            F V+IE+SS+QL + +ET +LSW VYRSDSA VT N QSS PD T G  ETPFV TSEG
Sbjct: 121 AFTVNIEISSMQLVSTDETLLLSWRVYRSDSAPVTSNFQSSPPDGTTGATETPFVKTSEG 180

Query: 187 KFAVELEFDAKHVPFYLSFLLKHPVGVDSGSSEIRSHRKTSFCVPVGFGRGYPSPLGVSI 246
           KF+VELEFDAKH PFYLSF+LK P+GVDSGSSEIRSHRKTSF VPVGFGRGYPSPLG+SI
Sbjct: 181 KFSVELEFDAKHTPFYLSFVLKFPMGVDSGSSEIRSHRKTSFSVPVGFGRGYPSPLGLSI 240

Query: 247 SGDGSMNFSIFSSSAESVVLCLYNDSTSEKPVIELDLDRYINRSGNIWHVSFESVSNFVS 306
           SGDGS+NFSIFSS+AESVVLCLYNDSTSEKP++ELDLD YINRSGNIWH SFE  SNFVS
Sbjct: 241 SGDGSINFSIFSSTAESVVLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGASNFVS 300

Query: 307 YGYLCKGSKSHKKQDGVEISRILVDPYAKVLTTSSHKTHGHGLGLPSKLLGQICKVPAFD 366
           YGY CKG KSH+ QDG+E+SRI+VDPYAK+L  S  K+   GLG P K LGQI  VPAFD
Sbjct: 301 YGYQCKGLKSHENQDGLEVSRIVVDPYAKILAPSIPKSRDQGLGFPLKFLGQISIVPAFD 360

Query: 367 WDGEVRPNLAMEKLFTYRLNVKRFTVDKSSQLTTDIAGTFAGLTKKLLHFKNLGVNAVLL 426
           WDGEVRPNL MEKLF YRLNVKRFT+DKSSQL  DIAGTF+GLTKKLLH KNLGVNAVLL
Sbjct: 361 WDGEVRPNLPMEKLFVYRLNVKRFTIDKSSQLPADIAGTFSGLTKKLLHLKNLGVNAVLL 420

Query: 427 EPIFPFDEKEGPYFPSHFFSPTNIYGPSGGSISAINSMKEMVKELHANGIEVMLEVVFTN 486
           EPIF FDEKEGPYFP HFFSPTN YGPSG SISAINSMKEMVKELHANG+EV++EVV+T+
Sbjct: 421 EPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVIVEVVYTH 480

Query: 487 TAEDGTLQGIDDSSYYFTNRVANLEGKNALNCNYPVVQQLLLDSLRYWVTEFHIDGFCFI 546
           T+ DG LQGIDDSSYYF NRVANLE ++ALNCNYP+VQQLLLDSLRYWVTEFH+DGFCF+
Sbjct: 481 TSVDGALQGIDDSSYYFANRVANLEERSALNCNYPIVQQLLLDSLRYWVTEFHVDGFCFV 540

Query: 547 NASSLLRGYRGELLSRPPLVEAIAFDPLLSKTKIIADFWDPHESISKETRFPHWKRWAEV 606
           NAS LL+G+ GELLSRPP VEAIAFDP+LSKTK++ADFWDP E +SKETRFPHWKRWAEV
Sbjct: 541 NASFLLQGHHGELLSRPPFVEAIAFDPILSKTKLVADFWDPQELVSKETRFPHWKRWAEV 600

Query: 607 NYKFCSDVRDFFRGEGLVSSLATRLCGSGDIFSDGRGPAFSFNFIARNFGLPLVDLVSFS 666
           N KFCSD+RDFFRGEGL+SSLATRLCGSGD+FSDGRGPAFSFNFIARN GLPLVDLVSFS
Sbjct: 601 NSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS 660

Query: 667 SSKLASELSWNCGEEGPTSNIEVLEKRLKLIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 726
           +S LASELSWNCGEEGPT+ ++VLEKRLK IRNFIFVLFVSLGVPVLNMGDECGQSSGGS
Sbjct: 661 NSNLASELSWNCGEEGPTNTLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 720

Query: 727 IAFKDRRPFDWDLLKTGFGTQTTQFITFFSSFRARRFDLFQSRNFLKGENIDWYGNNLSP 786
           +AF DRR F+WDLLKT FGTQTTQFI F SSFR+RRFDLFQSRNFLKGENIDW+ NN SP
Sbjct: 721 VAFNDRRSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLFQSRNFLKGENIDWFDNNQSP 780

Query: 787 PQWEDPSCKFLAVMLRADKEENGLSAENPEPQSDIFMAFNASDQSINVALPEPSEGTSWF 846
           PQWED SCKFLAVMLRADKEEN    ENP+ +SDIFMAFNASDQS +VALPEPSEGTSWF
Sbjct: 781 PQWEDASCKFLAVMLRADKEENESITENPKTRSDIFMAFNASDQSESVALPEPSEGTSWF 840

Query: 847 RLIDTALSFPGFFSSDGELVPTVESVTYEMQAHSCALFEAKS 889
           R++DTAL FPGFFSSDGELVP   SVTYEMQAHSCALFEAKS
Sbjct: 841 RVVDTALPFPGFFSSDGELVPMTGSVTYEMQAHSCALFEAKS 882

BLAST of Moc09g07920 vs. TAIR 10
Match: AT1G03310.2 (debranching enzyme 1 )

HSP 1 Score: 924.5 bits (2388), Expect = 6.6e-269
Identity = 459/824 (55.70%), Postives = 595/824 (72.21%), Query Frame = 0

Query: 72  YNLSKAYASFGISVEKNGWRFGISGKSKEQEKVATYLFRTECGDLVNVLVRKTGSGFMVS 131
           Y  SK  AS   S+E          ++ + +KV +Y FRT+ G LV V V K    + + 
Sbjct: 64  YQFSKICAS-KTSIELREALSSRRAEADDLKKVTSYSFRTKAGALVKVKVEKKREKYSIL 123

Query: 132 IELSSVQLANIEETPM-LSWGVYRSDSALVTP-NIQSSLPDDTIGTIETPFVNTSEGKFA 191
           + +SS++L+  +++ + + WGVYRSDS+   P + ++S  D    T ET FV +S  +  
Sbjct: 124 VYVSSLELSGDDKSRLVMVWGVYRSDSSCFLPLDFENSSQDSQTHTTETTFVKSSLSELM 183

Query: 192 VELEFDAKHVPFYLSFLLKHPVGVDSGSSEIRSHRKTSFCVPVGFGRGYPSPLGVSIS-G 251
           + LEFD K  PFYLSF LK   G D    E+ +HR T FC+PVGF  G+P PLG+S    
Sbjct: 184 LGLEFDGKESPFYLSFHLKLVSGRDPDGQEMLTHRDTDFCIPVGFTAGHPLPLGLSSGPD 243

Query: 252 DGSMNFSIFSSSAESVVLCLYNDSTSEKPVIELDLDRYINRSGNIWHVSFESVSNFVSYG 311
           D S NFS FS S+ +VVLCLY+DST++KP +ELDLD Y+NR+G++WH S ++  +FV YG
Sbjct: 244 DDSWNFSFFSRSSTNVVLCLYDDSTTDKPALELDLDPYVNRTGDVWHASVDNTWDFVRYG 303

Query: 312 YLCKGSKSHKKQDGVEISRILVDPYAKVLTTSSHKTHGHGLGLPSKLLGQICKVPAFDWD 371
           Y CK +   K+   VE   I++DPYA V+          G  +  K LG + K P+FDW 
Sbjct: 304 YRCKETAHSKEDVDVEGEPIVLDPYATVV----------GKSVSQKYLGSLSKSPSFDWG 363

Query: 372 GEVRPNLAMEKLFTYRLNVKRFTVDKSSQLTTDIAGTFAGLTKKLLHFKNLGVNAVLLEP 431
            +V PN+ +EKL  YRLNVK FT  +SS+L +++AGTF+G+ +K+ H K LG NAVLLEP
Sbjct: 364 EDVSPNIPLEKLLVYRLNVKGFTQHRSSKLPSNVAGTFSGVAEKVSHLKTLGTNAVLLEP 423

Query: 432 IFPFDEKEGPYFPSHFFSPTNIYGPSGGSISAINSMKEMVKELHANGIEVMLEVVFTNTA 491
           IF F E++GPYFP HFFSP +IYGPS    SA+NSMK MVK+LH+ GIEV+LEVVFT+TA
Sbjct: 424 IFSFSEQKGPYFPFHFFSPMDIYGPSNSLESAVNSMKVMVKKLHSEGIEVLLEVVFTHTA 483

Query: 492 EDGTLQGIDDSSYYFTNRVANLEGKNALNCNYPVVQQLLLDSLRYWVTEFHIDGFCFINA 551
           + G L+GIDDSSYY+  R  +L+ K+ LNCNYPVVQQL+L+SLRYWVTEFH+DGFCFINA
Sbjct: 484 DSGALRGIDDSSYYYKGRANDLDSKSYLNCNYPVVQQLVLESLRYWVTEFHVDGFCFINA 543

Query: 552 SSLLRGYRGELLSRPPLVEAIAFDPLLSKTKIIADFWDPHESISKETRFPHWKRWAEVNY 611
           SSLLRG  GE LSRPPLVEAIAFDPLL++TK+IAD WDP E + KE RFPHWKRWAE+N 
Sbjct: 544 SSLLRGVHGEQLSRPPLVEAIAFDPLLAETKLIADCWDPLEMMPKEVRFPHWKRWAELNT 603

Query: 612 KFCSDVRDFFRGEGLVSSLATRLCGSGDIFSDGRGPAFSFNFIARNFGLPLVDLVSFSSS 671
           ++C +VR+F RG G++S LATR+CGSGD+F+DGRGPAFSFN+I+RN GL LVD+VSFS  
Sbjct: 604 RYCRNVRNFLRGRGVLSDLATRICGSGDVFTDGRGPAFSFNYISRNSGLSLVDIVSFSGP 663

Query: 672 KLASELSWNCGEEGPTSNIEVLEKRLKLIRNFIFVLFVSLGVPVLNMGDECGQSSGGSIA 731
           +LASELSWNCGEEG T+   VL++RLK IRNF+F+ ++SLGVPVLNMGDECG S+ GS  
Sbjct: 664 ELASELSWNCGEEGATNKSAVLQRRLKQIRNFLFIQYISLGVPVLNMGDECGISTRGSPL 723

Query: 732 FKDRRPFDWDLLKTGFGTQTTQFITFFSSFRARRFDLFQSRNFLKGENIDWYGNNLSPPQ 791
            + R+PFDW+LL + FGTQ TQFI+F +S RARR D+FQ R+FLK ENI WY N+ + P+
Sbjct: 724 LESRKPFDWNLLASAFGTQITQFISFMTSVRARRSDVFQRRDFLKPENIVWYANDQTTPK 783

Query: 792 WEDPSCKFLAVMLRADKEENGLS--AENPEPQS-DIFMAFNASDQSINVALPEPSEGTSW 851
           WEDP+ KFLA+ ++++ EE   +  AE  EP+S D+F+ FNASD   +V LP   +G+ W
Sbjct: 784 WEDPASKFLALEIKSESEEEETASLAEPNEPKSNDLFIGFNASDHPESVVLPSLPDGSKW 843

Query: 852 FRLIDTALSFPGFFSSDGELV----PTVESVTYEMQAHSCALFE 886
            RL+DTAL FPGFFS +GE V    P  + V YEM+ +SC LFE
Sbjct: 844 RRLVDTALPFPGFFSVEGETVVAEEPLQQLVVYEMKPYSCTLFE 876

BLAST of Moc09g07920 vs. TAIR 10
Match: AT1G03310.1 (debranching enzyme 1 )

HSP 1 Score: 924.5 bits (2388), Expect = 6.6e-269
Identity = 459/824 (55.70%), Postives = 595/824 (72.21%), Query Frame = 0

Query: 72  YNLSKAYASFGISVEKNGWRFGISGKSKEQEKVATYLFRTECGDLVNVLVRKTGSGFMVS 131
           Y  SK  AS   S+E          ++ + +KV +Y FRT+ G LV V V K    + + 
Sbjct: 64  YQFSKICAS-KTSIELREALSSRRAEADDLKKVTSYSFRTKAGALVKVKVEKKREKYSIL 123

Query: 132 IELSSVQLANIEETPM-LSWGVYRSDSALVTP-NIQSSLPDDTIGTIETPFVNTSEGKFA 191
           + +SS++L+  +++ + + WGVYRSDS+   P + ++S  D    T ET FV +S  +  
Sbjct: 124 VYVSSLELSGDDKSRLVMVWGVYRSDSSCFLPLDFENSSQDSQTHTTETTFVKSSLSELM 183

Query: 192 VELEFDAKHVPFYLSFLLKHPVGVDSGSSEIRSHRKTSFCVPVGFGRGYPSPLGVSIS-G 251
           + LEFD K  PFYLSF LK   G D    E+ +HR T FC+PVGF  G+P PLG+S    
Sbjct: 184 LGLEFDGKESPFYLSFHLKLVSGRDPDGQEMLTHRDTDFCIPVGFTAGHPLPLGLSSGPD 243

Query: 252 DGSMNFSIFSSSAESVVLCLYNDSTSEKPVIELDLDRYINRSGNIWHVSFESVSNFVSYG 311
           D S NFS FS S+ +VVLCLY+DST++KP +ELDLD Y+NR+G++WH S ++  +FV YG
Sbjct: 244 DDSWNFSFFSRSSTNVVLCLYDDSTTDKPALELDLDPYVNRTGDVWHASVDNTWDFVRYG 303

Query: 312 YLCKGSKSHKKQDGVEISRILVDPYAKVLTTSSHKTHGHGLGLPSKLLGQICKVPAFDWD 371
           Y CK +   K+   VE   I++DPYA V+          G  +  K LG + K P+FDW 
Sbjct: 304 YRCKETAHSKEDVDVEGEPIVLDPYATVV----------GKSVSQKYLGSLSKSPSFDWG 363

Query: 372 GEVRPNLAMEKLFTYRLNVKRFTVDKSSQLTTDIAGTFAGLTKKLLHFKNLGVNAVLLEP 431
            +V PN+ +EKL  YRLNVK FT  +SS+L +++AGTF+G+ +K+ H K LG NAVLLEP
Sbjct: 364 EDVSPNIPLEKLLVYRLNVKGFTQHRSSKLPSNVAGTFSGVAEKVSHLKTLGTNAVLLEP 423

Query: 432 IFPFDEKEGPYFPSHFFSPTNIYGPSGGSISAINSMKEMVKELHANGIEVMLEVVFTNTA 491
           IF F E++GPYFP HFFSP +IYGPS    SA+NSMK MVK+LH+ GIEV+LEVVFT+TA
Sbjct: 424 IFSFSEQKGPYFPFHFFSPMDIYGPSNSLESAVNSMKVMVKKLHSEGIEVLLEVVFTHTA 483

Query: 492 EDGTLQGIDDSSYYFTNRVANLEGKNALNCNYPVVQQLLLDSLRYWVTEFHIDGFCFINA 551
           + G L+GIDDSSYY+  R  +L+ K+ LNCNYPVVQQL+L+SLRYWVTEFH+DGFCFINA
Sbjct: 484 DSGALRGIDDSSYYYKGRANDLDSKSYLNCNYPVVQQLVLESLRYWVTEFHVDGFCFINA 543

Query: 552 SSLLRGYRGELLSRPPLVEAIAFDPLLSKTKIIADFWDPHESISKETRFPHWKRWAEVNY 611
           SSLLRG  GE LSRPPLVEAIAFDPLL++TK+IAD WDP E + KE RFPHWKRWAE+N 
Sbjct: 544 SSLLRGVHGEQLSRPPLVEAIAFDPLLAETKLIADCWDPLEMMPKEVRFPHWKRWAELNT 603

Query: 612 KFCSDVRDFFRGEGLVSSLATRLCGSGDIFSDGRGPAFSFNFIARNFGLPLVDLVSFSSS 671
           ++C +VR+F RG G++S LATR+CGSGD+F+DGRGPAFSFN+I+RN GL LVD+VSFS  
Sbjct: 604 RYCRNVRNFLRGRGVLSDLATRICGSGDVFTDGRGPAFSFNYISRNSGLSLVDIVSFSGP 663

Query: 672 KLASELSWNCGEEGPTSNIEVLEKRLKLIRNFIFVLFVSLGVPVLNMGDECGQSSGGSIA 731
           +LASELSWNCGEEG T+   VL++RLK IRNF+F+ ++SLGVPVLNMGDECG S+ GS  
Sbjct: 664 ELASELSWNCGEEGATNKSAVLQRRLKQIRNFLFIQYISLGVPVLNMGDECGISTRGSPL 723

Query: 732 FKDRRPFDWDLLKTGFGTQTTQFITFFSSFRARRFDLFQSRNFLKGENIDWYGNNLSPPQ 791
            + R+PFDW+LL + FGTQ TQFI+F +S RARR D+FQ R+FLK ENI WY N+ + P+
Sbjct: 724 LESRKPFDWNLLASAFGTQITQFISFMTSVRARRSDVFQRRDFLKPENIVWYANDQTTPK 783

Query: 792 WEDPSCKFLAVMLRADKEENGLS--AENPEPQS-DIFMAFNASDQSINVALPEPSEGTSW 851
           WEDP+ KFLA+ ++++ EE   +  AE  EP+S D+F+ FNASD   +V LP   +G+ W
Sbjct: 784 WEDPASKFLALEIKSESEEEETASLAEPNEPKSNDLFIGFNASDHPESVVLPSLPDGSKW 843

Query: 852 FRLIDTALSFPGFFSSDGELV----PTVESVTYEMQAHSCALFE 886
            RL+DTAL FPGFFS +GE V    P  + V YEM+ +SC LFE
Sbjct: 844 RRLVDTALPFPGFFSVEGETVVAEEPLQQLVVYEMKPYSCTLFE 876

BLAST of Moc09g07920 vs. TAIR 10
Match: AT4G09020.1 (isoamylase 3 )

HSP 1 Score: 375.2 bits (962), Expect = 1.5e-103
Identity = 254/704 (36.08%), Postives = 363/704 (51.56%), Query Frame = 0

Query: 237 GYPSPLGVSISGDGSMNFSIFSSSAESVVLCLY-----NDSTSEKPVIELDLDRYINRSG 296
           G  SPLGVS   D  +NF++FS +A SV LCL       D T +  +IEL LD  +N++G
Sbjct: 88  GEVSPLGVS-QVDKGINFALFSQNATSVTLCLSLSQSGKDDTDDDGMIELVLDPSVNKTG 147

Query: 297 NIWHVSFESVS-NFVSYGYLCKGSKSHKKQDGVEISRILVDPYAKVLTTSSHKTHGHGLG 356
           + WH+  E +  N V YGY   G    ++    + S +L+DPYAK++    H + G    
Sbjct: 148 DTWHICVEDLPLNNVLYGYRVDGPGEWQQGHRFDRSILLLDPYAKLV--KGHSSFGDSSQ 207

Query: 357 LPSKLLGQI-CKVPAFDWDGEVR-PNLAMEKLFTYRLNVKRFTVDKSSQLTTDIAGTFAG 416
             ++  G    +   FDW  + + PN+  + L  Y +NV+ FT D+SS +   I G++ G
Sbjct: 208 KFAQFYGTYDFESSPFDWGDDYKFPNIPEKDLVIYEMNVRAFTADESSGMDPAIGGSYLG 267

Query: 417 LTKKLLHFKNLGVNAVLLEPIFPFDEKE--------------GPYFPSHFFSPTNIYGP- 476
             +K+ H ++LG+NAV L P+F FDE E                Y   +FF+P + Y   
Sbjct: 268 FIEKIPHLQDLGINAVELLPVFEFDELELQRRSNPRDHMVNTWGYSTVNFFAPMSRYASG 327

Query: 477 SGGSISAINSMKEMVKELHANGIEVMLEVVF--TNTAED-----GTLQGIDDSSYYF--- 536
            G  I A    KEMVK LH+ GIEV+L+VV+  TN A+D      + +GID+  YY    
Sbjct: 328 EGDPIKASKEFKEMVKALHSAGIEVILDVVYNHTNEADDKYPYTTSFRGIDNKVYYMLDP 387

Query: 537 TNRVANLEG-KNALNCNYPVVQQLLLDSLRYWVTEFHIDGFCFINASSLLRGYRGELLSR 596
            N++ N  G  N LNCN+PVV +L+LDSLR+WVTE+H+DGF F  AS L R   G  LS 
Sbjct: 388 NNQLLNFSGCGNTLNCNHPVVMELILDSLRHWVTEYHVDGFRFDLASVLCRATDGSPLSA 447

Query: 597 PPLVEAIAFDPLLSKTKIIADFWDPHESISKETRFPHWKRWAEVNYKFCSDVRDFFRGE- 656
           PPL+ AIA D +LS+ KIIA+ WD    +    +FP+W RWAE N  +  DVR F +G+ 
Sbjct: 448 PPLIRAIAKDSVLSRCKIIAEPWD-CGGLYLVGKFPNWDRWAEWNGMYRDDVRRFIKGDS 507

Query: 657 GLVSSLATRLCGSGDIFS-DGRGPAFSFNFIARNFGLPLVDLVSFS-----------SSK 716
           G+  S ATR+ GS D++  + R P    NF+  + G  L DLVS++           +  
Sbjct: 508 GMKGSFATRVSGSSDLYQVNQRKPYHGVNFVIAHDGFTLRDLVSYNFKHNEANGEGGNDG 567

Query: 717 LASELSWNCGEEGPTSNIEVLEKRLKLIRNFIFVLFVSLGVPVLNMGDECGQSS-GGSIA 776
                SWNCG EG T +  +   R + ++NF   L +S G P++ MGDE G +  G + +
Sbjct: 568 CNDNHSWNCGFEGETGDAHIKSLRTRQMKNFHLALMISQGTPMMLMGDEYGHTRYGNNNS 627

Query: 777 F---KDRRPFDWDLLKTGFGTQTTQFITFFSSFRARRFD--LFQSRNFLKGENIDWYGNN 836
           +        F W  L      +      FFS     R    + +  NFL    I W+ +N
Sbjct: 628 YGHDTSLNNFQWKEL----DAKKQNHFRFFSEVIKFRHSHHVLKHENFLTQGEITWHEDN 687

Query: 837 LSPPQWEDPSCKFLAVMLRADKEENGLSAENPEPQSDIFMAFNASDQSINVALPEPSEGT 888
                W++   KFLA  L      +G+         DI++AFNA D  +   +P+P  G 
Sbjct: 688 -----WDNSESKFLAFTL-----HDGIGGR------DIYVAFNAHDYFVKALIPQPPPGK 747

BLAST of Moc09g07920 vs. TAIR 10
Match: AT2G39930.1 (isoamylase 1 )

HSP 1 Score: 323.2 bits (827), Expect = 6.7e-88
Identity = 229/690 (33.19%), Postives = 335/690 (48.55%), Query Frame = 0

Query: 237 GYPSPLGVSISGDGSMNFSIFSSSAESVVLCL--YNDSTSEKPVIELDLDRYINRSGNIW 296
           G PSP G ++  DG +NFS++S+++ S  +CL   +D    K   E+ LD   NR+G++W
Sbjct: 72  GLPSPFGPTVRDDG-VNFSVYSTNSVSATICLISLSDLRQNKVTEEIQLDPSRNRTGHVW 131

Query: 297 HVSFESVSNFVSYGYLCKGSKSHKKQDGVEISRILVDPYAKVLTTSSHKTHGHGLGLPSK 356
           HV        + YGY   G  S ++    + S IL+DPYAK + +   +     LG    
Sbjct: 132 HVFLRGDFKDMLYGYRFDGKFSPEEGHYYDSSNILLDPYAKAIIS---RDEFGVLGPDDN 191

Query: 357 LLGQI-CKVPA----FDWDGEVRPNLAMEKLFTYRLNVKRFTVDKSSQLTTDIAGTFAGL 416
              Q+ C VP     FDW+G++   L  + L  Y ++V+ FT  +SS++  +  GT+ G+
Sbjct: 192 CWPQMACMVPTREEEFDWEGDMHLKLPQKDLVIYEMHVRGFTRHESSKI--EFPGTYQGV 251

Query: 417 TKKLLHFKNLGVNAVLLEPIFPFDEKE---------------GPYFPSHFFSPTNIYGPS 476
            +KL H K LG+N + L P   F+E E                 Y    FFSP   Y  +
Sbjct: 252 AEKLDHLKELGINCIELMPCHEFNELEYYSYNTILGDHRVNFWGYSTIGFFSPMIRYASA 311

Query: 477 GGSI---SAINSMKEMVKELHANGIEVMLEVVFTNTAEDG------TLQGIDDSSYYF-- 536
             +     AIN  K +VKE H  GIEV+++VV  +TAE        + +G+D+S YY   
Sbjct: 312 SSNNFAGRAINEFKILVKEAHKRGIEVIMDVVLNHTAEGNEKGPIFSFRGVDNSVYYMLA 371

Query: 537 -TNRVANLEG-KNALNCNYPVVQQLLLDSLRYWVTEFHIDGFCFINASSLLRG------- 596
                 N  G  N  NCN+PVV+Q +LD LRYWVTE H+DGF F   S + R        
Sbjct: 372 PKGEFYNYSGCGNTFNCNHPVVRQFILDCLRYWVTEMHVDGFRFDLGSIMSRSSSLWDAA 431

Query: 597 ------YRGELL------SRPPLVEAIAFDPLLSKTKIIADFWDPHESISKETRFPHWKR 656
                   G+LL      S PP+++ I+ DP+L   K+IA+ WD    + +   FPHW  
Sbjct: 432 NVYGADVEGDLLTTGTPISCPPVIDMISNDPILRGVKLIAEAWDA-GGLYQVGMFPHWGI 491

Query: 657 WAEVNYKFCSDVRDFFRG-EGLVSSLATRLCGSGDIFSDGRGPAFSFNFIARNFGLPLVD 716
           W+E N KF   VR F +G +G   + A  LCGS +++  GR P  S NFI  + G  L D
Sbjct: 492 WSEWNGKFRDVVRQFIKGTDGFSGAFAECLCGSPNLYQGGRKPWHSINFICAHDGFTLAD 551

Query: 717 LVSFSS-SKLAS----------ELSWNCGEEGPTSNIEVLEKRLKLIRNFIFVLFVSLGV 776
           LV++++ + LA+            SWNCGEEG  ++I V   R + +RNF   L VS GV
Sbjct: 552 LVTYNNKNNLANGEENNDGENHNYSWNCGEEGDFASISVKRLRKRQMRNFFVSLMVSQGV 611

Query: 777 PVLNMGDECGQSSGGS----IAFKDRRPFDWDLLKTGFGTQTTQFITFFSSFRARRFDLF 836
           P++ MGDE G + GG+            F WD  K    +   +F      FR    +  
Sbjct: 612 PMIYMGDEYGHTKGGNNNTYCHDNYMNYFRWD-KKEEAHSDFFRFCRILIKFR-DECESL 671

Query: 837 QSRNFLKGENIDWYGNNLSPPQWEDPSCKFLAVMLRADKEENGLSAENPEPQSDIFMAFN 857
              +F   + + W+G     P W + S +F+A  L                + +I++AFN
Sbjct: 672 GLNDFPTAKRLQWHGLAPEIPNWSETS-RFVAFSL------------VDSVKKEIYVAFN 731

BLAST of Moc09g07920 vs. TAIR 10
Match: AT3G20440.2 (Alpha amylase family protein )

HSP 1 Score: 55.5 bits (132), Expect = 2.6e-07
Identity = 43/161 (26.71%), Postives = 74/161 (45.96%), Query Frame = 0

Query: 405 TFAGLTKKLL-HFKNLGVNAVLLEPIFPF-DEKEGPYFPSHFFSPTNIYGPSGGSISAIN 464
           TF   TKK+L H K  G NA+ L  +    D     Y  ++FF+ ++ YG         +
Sbjct: 416 TFEEFTKKVLPHVKRAGYNAIQLIGVPEHKDYFTVGYRVTNFFAASSRYGTP-------D 475

Query: 465 SMKEMVKELHANGIEVMLEVVFTNTAED-----GTLQGIDDSSYYFTNRVANLE-GKNAL 524
             K +V E H  G+ V L++V +  A D         G +D  +++  R  +   G    
Sbjct: 476 DFKRLVDEAHGLGLLVFLDIVHSYAAADQMVGLSLFDGSNDCYFHYGKRGHHKHWGTRMF 535

Query: 525 NCNYPVVQQLLLDSLRYWVTEFHIDGFCFINASSLLRGYRG 558
                 V   L+ +L +W+TE+ +DG+ F + +S++  + G
Sbjct: 536 KYGDLDVLHFLISNLNWWITEYQVDGYQFHSLASMIYTHNG 569