Homology
BLAST of Moc09g07920 vs. NCBI nr
Match:
XP_022150231.1 (isoamylase 2, chloroplastic [Momordica charantia])
HSP 1 Score: 1814.3 bits (4698), Expect = 0.0e+00
Identity = 891/891 (100.00%), Postives = 891/891 (100.00%), Query Frame = 0
Query: 1 MLLQPQMATFPSSLVVQPYNVHNFGVTEAFRLAASDHHTHGHMIKHQSGKRDEVRMLAHG 60
MLLQPQMATFPSSLVVQPYNVHNFGVTEAFRLAASDHHTHGHMIKHQSGKRDEVRMLAHG
Sbjct: 1 MLLQPQMATFPSSLVVQPYNVHNFGVTEAFRLAASDHHTHGHMIKHQSGKRDEVRMLAHG 60
Query: 61 ESRAGFAKSSRYNLSKAYASFGISVEKNGWRFGISGKSKEQEKVATYLFRTECGDLVNVL 120
ESRAGFAKSSRYNLSKAYASFGISVEKNGWRFGISGKSKEQEKVATYLFRTECGDLVNVL
Sbjct: 61 ESRAGFAKSSRYNLSKAYASFGISVEKNGWRFGISGKSKEQEKVATYLFRTECGDLVNVL 120
Query: 121 VRKTGSGFMVSIELSSVQLANIEETPMLSWGVYRSDSALVTPNIQSSLPDDTIGTIETPF 180
VRKTGSGFMVSIELSSVQLANIEETPMLSWGVYRSDSALVTPNIQSSLPDDTIGTIETPF
Sbjct: 121 VRKTGSGFMVSIELSSVQLANIEETPMLSWGVYRSDSALVTPNIQSSLPDDTIGTIETPF 180
Query: 181 VNTSEGKFAVELEFDAKHVPFYLSFLLKHPVGVDSGSSEIRSHRKTSFCVPVGFGRGYPS 240
VNTSEGKFAVELEFDAKHVPFYLSFLLKHPVGVDSGSSEIRSHRKTSFCVPVGFGRGYPS
Sbjct: 181 VNTSEGKFAVELEFDAKHVPFYLSFLLKHPVGVDSGSSEIRSHRKTSFCVPVGFGRGYPS 240
Query: 241 PLGVSISGDGSMNFSIFSSSAESVVLCLYNDSTSEKPVIELDLDRYINRSGNIWHVSFES 300
PLGVSISGDGSMNFSIFSSSAESVVLCLYNDSTSEKPVIELDLDRYINRSGNIWHVSFES
Sbjct: 241 PLGVSISGDGSMNFSIFSSSAESVVLCLYNDSTSEKPVIELDLDRYINRSGNIWHVSFES 300
Query: 301 VSNFVSYGYLCKGSKSHKKQDGVEISRILVDPYAKVLTTSSHKTHGHGLGLPSKLLGQIC 360
VSNFVSYGYLCKGSKSHKKQDGVEISRILVDPYAKVLTTSSHKTHGHGLGLPSKLLGQIC
Sbjct: 301 VSNFVSYGYLCKGSKSHKKQDGVEISRILVDPYAKVLTTSSHKTHGHGLGLPSKLLGQIC 360
Query: 361 KVPAFDWDGEVRPNLAMEKLFTYRLNVKRFTVDKSSQLTTDIAGTFAGLTKKLLHFKNLG 420
KVPAFDWDGEVRPNLAMEKLFTYRLNVKRFTVDKSSQLTTDIAGTFAGLTKKLLHFKNLG
Sbjct: 361 KVPAFDWDGEVRPNLAMEKLFTYRLNVKRFTVDKSSQLTTDIAGTFAGLTKKLLHFKNLG 420
Query: 421 VNAVLLEPIFPFDEKEGPYFPSHFFSPTNIYGPSGGSISAINSMKEMVKELHANGIEVML 480
VNAVLLEPIFPFDEKEGPYFPSHFFSPTNIYGPSGGSISAINSMKEMVKELHANGIEVML
Sbjct: 421 VNAVLLEPIFPFDEKEGPYFPSHFFSPTNIYGPSGGSISAINSMKEMVKELHANGIEVML 480
Query: 481 EVVFTNTAEDGTLQGIDDSSYYFTNRVANLEGKNALNCNYPVVQQLLLDSLRYWVTEFHI 540
EVVFTNTAEDGTLQGIDDSSYYFTNRVANLEGKNALNCNYPVVQQLLLDSLRYWVTEFHI
Sbjct: 481 EVVFTNTAEDGTLQGIDDSSYYFTNRVANLEGKNALNCNYPVVQQLLLDSLRYWVTEFHI 540
Query: 541 DGFCFINASSLLRGYRGELLSRPPLVEAIAFDPLLSKTKIIADFWDPHESISKETRFPHW 600
DGFCFINASSLLRGYRGELLSRPPLVEAIAFDPLLSKTKIIADFWDPHESISKETRFPHW
Sbjct: 541 DGFCFINASSLLRGYRGELLSRPPLVEAIAFDPLLSKTKIIADFWDPHESISKETRFPHW 600
Query: 601 KRWAEVNYKFCSDVRDFFRGEGLVSSLATRLCGSGDIFSDGRGPAFSFNFIARNFGLPLV 660
KRWAEVNYKFCSDVRDFFRGEGLVSSLATRLCGSGDIFSDGRGPAFSFNFIARNFGLPLV
Sbjct: 601 KRWAEVNYKFCSDVRDFFRGEGLVSSLATRLCGSGDIFSDGRGPAFSFNFIARNFGLPLV 660
Query: 661 DLVSFSSSKLASELSWNCGEEGPTSNIEVLEKRLKLIRNFIFVLFVSLGVPVLNMGDECG 720
DLVSFSSSKLASELSWNCGEEGPTSNIEVLEKRLKLIRNFIFVLFVSLGVPVLNMGDECG
Sbjct: 661 DLVSFSSSKLASELSWNCGEEGPTSNIEVLEKRLKLIRNFIFVLFVSLGVPVLNMGDECG 720
Query: 721 QSSGGSIAFKDRRPFDWDLLKTGFGTQTTQFITFFSSFRARRFDLFQSRNFLKGENIDWY 780
QSSGGSIAFKDRRPFDWDLLKTGFGTQTTQFITFFSSFRARRFDLFQSRNFLKGENIDWY
Sbjct: 721 QSSGGSIAFKDRRPFDWDLLKTGFGTQTTQFITFFSSFRARRFDLFQSRNFLKGENIDWY 780
Query: 781 GNNLSPPQWEDPSCKFLAVMLRADKEENGLSAENPEPQSDIFMAFNASDQSINVALPEPS 840
GNNLSPPQWEDPSCKFLAVMLRADKEENGLSAENPEPQSDIFMAFNASDQSINVALPEPS
Sbjct: 781 GNNLSPPQWEDPSCKFLAVMLRADKEENGLSAENPEPQSDIFMAFNASDQSINVALPEPS 840
Query: 841 EGTSWFRLIDTALSFPGFFSSDGELVPTVESVTYEMQAHSCALFEAKSYSD 892
EGTSWFRLIDTALSFPGFFSSDGELVPTVESVTYEMQAHSCALFEAKSYSD
Sbjct: 841 EGTSWFRLIDTALSFPGFFSSDGELVPTVESVTYEMQAHSCALFEAKSYSD 891
BLAST of Moc09g07920 vs. NCBI nr
Match:
XP_038898155.1 (isoamylase 2, chloroplastic [Benincasa hispida])
HSP 1 Score: 1513.0 bits (3916), Expect = 0.0e+00
Identity = 740/885 (83.62%), Postives = 796/885 (89.94%), Query Frame = 0
Query: 7 MATFPSSLVVQPYNVHNFGVTEAFRLAASDHHTHGHMIKHQSGKRDEVRMLAHGESRAGF 66
MATF SSLV+QP+ V+N GVTE+F+LA DHHT+G IK+ SGK DE RMLAHGESR G
Sbjct: 1 MATFLSSLVIQPFCVYNSGVTESFKLAVGDHHTYGQKIKYHSGKMDEARMLAHGESRVGS 60
Query: 67 AKSSRYNLSKAYASFGISVEKNGWRFGISGKSKEQEKVATYLFRTECGDLVNVLVRKTGS 126
KSS NLSK YA+ GI V K+G RFG GKSKE KVATYLFRTE GDLVNV V KTGS
Sbjct: 61 TKSSHRNLSKVYATSGILVGKSGQRFGTGGKSKEHVKVATYLFRTEFGDLVNVFVGKTGS 120
Query: 127 GFMVSIELSSVQLANIEETPMLSWGVYRSDSALVTPNIQSSLPDDTIGTIETPFVNTSEG 186
GFMV++E+SSVQLA+IE+T +LSWGVYRSDSA+VTPN QSSLPD+TIG ETPFV TS G
Sbjct: 121 GFMVNVEISSVQLASIEKTLLLSWGVYRSDSAIVTPNFQSSLPDETIGATETPFVKTSGG 180
Query: 187 KFAVELEFDAKHVPFYLSFLLKHPVGVDSGSSEIRSHRKTSFCVPVGFGRGYPSPLGVSI 246
KFAVELEFDAKH PFYLSFLLK+PVGVDSGSSEIRSHRKTSF VPVGFGRGYPSPLG+SI
Sbjct: 181 KFAVELEFDAKHTPFYLSFLLKYPVGVDSGSSEIRSHRKTSFTVPVGFGRGYPSPLGLSI 240
Query: 247 SGDGSMNFSIFSSSAESVVLCLYNDSTSEKPVIELDLDRYINRSGNIWHVSFESVSNFVS 306
SGDGS+NFSIFSS+AESVVLCLYNDSTSEKPV+ELDLD YINRSG+IWH SFES SNFVS
Sbjct: 241 SGDGSINFSIFSSTAESVVLCLYNDSTSEKPVLELDLDSYINRSGSIWHASFESASNFVS 300
Query: 307 YGYLCKGSKSHKKQDGVEISRILVDPYAKVLTTSSHKTHGHGLGLPSKLLGQICKVPAFD 366
YGY CKGSK H+ QDGVE++RI+VDPYAK+LT S KT G GLGLP K LGQICKVPAFD
Sbjct: 301 YGYRCKGSKFHENQDGVEVNRIVVDPYAKILTASVAKTLGQGLGLP-KFLGQICKVPAFD 360
Query: 367 WDGEVRPNLAMEKLFTYRLNVKRFTVDKSSQLTTDIAGTFAGLTKKLLHFKNLGVNAVLL 426
WDGEV PNL MEKLF YRLNVKRFTVDKSSQL DIAGTF+GLTKKLLHFKNLGVNAVLL
Sbjct: 361 WDGEVCPNLPMEKLFAYRLNVKRFTVDKSSQLPADIAGTFSGLTKKLLHFKNLGVNAVLL 420
Query: 427 EPIFPFDEKEGPYFPSHFFSPTNIYGPSGGSISAINSMKEMVKELHANGIEVMLEVVFTN 486
EPIF FDEKEG YFP HFFSP N YGPSG SISAINSMK MVK+LHANG+EV+LEVVFT+
Sbjct: 421 EPIFQFDEKEGLYFPFHFFSPANNYGPSGVSISAINSMKGMVKQLHANGVEVILEVVFTH 480
Query: 487 TAEDGTLQGIDDSSYYFTNRVANLEGKNALNCNYPVVQQLLLDSLRYWVTEFHIDGFCFI 546
TA DG LQGIDDSSYYF NRVANLEGK+ALNCNYP+VQQLLLDSLRYWVTEFHIDGFCFI
Sbjct: 481 TAMDGALQGIDDSSYYFVNRVANLEGKSALNCNYPIVQQLLLDSLRYWVTEFHIDGFCFI 540
Query: 547 NASSLLRGYRGELLSRPPLVEAIAFDPLLSKTKIIADFWDPHESISKETRFPHWKRWAEV 606
NASSLLRG+ GE LSR P VEAIAFDPLLSKTK++ADFWDP E +S+ET FPHWKRWAEV
Sbjct: 541 NASSLLRGHHGEFLSRSPFVEAIAFDPLLSKTKLVADFWDPQELVSEETSFPHWKRWAEV 600
Query: 607 NYKFCSDVRDFFRGEGLVSSLATRLCGSGDIFSDGRGPAFSFNFIARNFGLPLVDLVSFS 666
N KFC+DVRDFFRGEGL+SSLATRLCGSGD+FS+GRGPAFSFNFI RN GLPLVDLVSFS
Sbjct: 601 NSKFCNDVRDFFRGEGLISSLATRLCGSGDVFSNGRGPAFSFNFITRNVGLPLVDLVSFS 660
Query: 667 SSKLASELSWNCGEEGPTSNIEVLEKRLKLIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 726
+SKLASELSWNCGEEGPTSNIEVLEKRLK IRNFIFV+FVSLGVPVLNMGDECGQS+GGS
Sbjct: 661 NSKLASELSWNCGEEGPTSNIEVLEKRLKQIRNFIFVVFVSLGVPVLNMGDECGQSAGGS 720
Query: 727 IAFKDRRPFDWDLLKTGFGTQTTQFITFFSSFRARRFDLFQSRNFLKGENIDWYGNNLSP 786
+AF DRR F+WDLLKT FGTQTTQFITF SSFR+RRFDLFQSRNFLKGENIDW+ NN SP
Sbjct: 721 VAFNDRRSFNWDLLKTDFGTQTTQFITFLSSFRSRRFDLFQSRNFLKGENIDWFDNNQSP 780
Query: 787 PQWEDPSCKFLAVMLRADKEENGLSAENPEPQSDIFMAFNASDQSINVALPEPSEGTSWF 846
PQWEDPSCKFLAVMLRADKEENG EN +P+SDIFMAFNASDQS VALPEPSEGTSWF
Sbjct: 781 PQWEDPSCKFLAVMLRADKEENGSITENAKPRSDIFMAFNASDQSETVALPEPSEGTSWF 840
Query: 847 RLIDTALSFPGFFSSDGELVPTVESVTYEMQAHSCALFEAKSYSD 892
RL+DTALSFPGFFSSDGE VPT SVTYEMQAHSCALFEAKS++D
Sbjct: 841 RLVDTALSFPGFFSSDGEQVPTTGSVTYEMQAHSCALFEAKSFND 884
BLAST of Moc09g07920 vs. NCBI nr
Match:
KAG7014422.1 (Isoamylase 2, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])
HSP 1 Score: 1479.2 bits (3828), Expect = 0.0e+00
Identity = 718/890 (80.67%), Postives = 795/890 (89.33%), Query Frame = 0
Query: 2 LLQPQMATFPSSLVVQPYNVHNFGVTEAFRLAASDHHTHGHMIKHQSGKRDEVRMLAHGE 61
L Q QMATF SSL +QP+ +N G TE+F+LA +D+HT+G + +QSGK DE RM AHGE
Sbjct: 19 LYQFQMATFLSSL-IQPFCGYNRGGTESFKLAVNDYHTYGQKMNYQSGKLDEARMQAHGE 78
Query: 62 SRAGFAKSSRYNLSKAYASFGISVEKNGWRFGISGKSKEQEKVATYLFRTECGDLVNVLV 121
S+ GF KS N+ KAYA GISV K+G +FG GK++E KVATYLFRTE GDLV V
Sbjct: 79 SKVGFTKSLHRNVCKAYAPSGISVGKSGQKFGTGGKTEEDRKVATYLFRTEFGDLVYAFV 138
Query: 122 RKTGSGFMVSIELSSVQLANIEETPMLSWGVYRSDSALVTPNIQSSLPDDTIGTIETPFV 181
K GSGF+VSIE+SS++LA+I+ET +LSWGVYRS+SA+VTPN QS LP++TIG ETPFV
Sbjct: 139 GKRGSGFLVSIEISSLKLASIDETLLLSWGVYRSNSAIVTPNFQSILPEETIGATETPFV 198
Query: 182 NTSEGKFAVELEFDAKHVPFYLSFLLKHPVGVDSGSSEIRSHRKTSFCVPVGFGRGYPSP 241
TSEGKFAVELEFDAKH PFYLSFLLK+PVGVDSGSSEI+SHRKTSF VPVGFGRGYPSP
Sbjct: 199 RTSEGKFAVELEFDAKHDPFYLSFLLKYPVGVDSGSSEIKSHRKTSFSVPVGFGRGYPSP 258
Query: 242 LGVSISGDGSMNFSIFSSSAESVVLCLYNDSTSEKPVIELDLDRYINRSGNIWHVSFESV 301
LG+SISGDGS+NFSIFSS+AESVVLCLYNDSTSEKPV+ELDLD YINRSGNIWHVSFE
Sbjct: 259 LGLSISGDGSINFSIFSSTAESVVLCLYNDSTSEKPVLELDLDPYINRSGNIWHVSFEGA 318
Query: 302 SNFVSYGYLCKGSKSHKKQDGVEISRILVDPYAKVLTTSSHKTHGHGLGLPSKLLGQICK 361
SNFVSYGY C+GSK H+ QD E+SRILVDPYAK+LT S+ KT G G+GLPSK LGQIC
Sbjct: 319 SNFVSYGYRCRGSKFHENQDEAEVSRILVDPYAKILTASTPKTRGQGVGLPSKFLGQICN 378
Query: 362 VPAFDWDGEVRPNLAMEKLFTYRLNVKRFTVDKSSQLTTDIAGTFAGLTKKLLHFKNLGV 421
VPAF WDGEVRPNL MEKLF YRLNVK FTVDKSSQL DIAGTF+GLT+KLLHFK LGV
Sbjct: 379 VPAFSWDGEVRPNLPMEKLFIYRLNVKHFTVDKSSQLPIDIAGTFSGLTQKLLHFKKLGV 438
Query: 422 NAVLLEPIFPFDEKEGPYFPSHFFSPTNIYGPSGGSISAINSMKEMVKELHANGIEVMLE 481
NAVLLEPIFPFDEK+GPYFP HFFSPTNIY PSG SIS++NS+KEMVKELHANG+EV+LE
Sbjct: 439 NAVLLEPIFPFDEKDGPYFPFHFFSPTNIYVPSGASISSLNSVKEMVKELHANGVEVILE 498
Query: 482 VVFTNTAEDGTLQGIDDSSYYFTNRVANLEGKNALNCNYPVVQQLLLDSLRYWVTEFHID 541
VVFT+TA DG LQGIDDS YYF NRVAN EGK+ALNCN+P+VQQLLLDSLRYWVTEFHID
Sbjct: 499 VVFTHTAMDGALQGIDDSCYYFVNRVANSEGKSALNCNHPIVQQLLLDSLRYWVTEFHID 558
Query: 542 GFCFINASSLLRGYRGELLSRPPLVEAIAFDPLLSKTKIIADFWDPHESISKETRFPHWK 601
GFCFINASSLL+G+ GELLSRPP VEAIAFDPLLSKTKIIA+FWDP E + KETRFPHWK
Sbjct: 559 GFCFINASSLLQGHHGELLSRPPFVEAIAFDPLLSKTKIIAEFWDPQELVPKETRFPHWK 618
Query: 602 RWAEVNYKFCSDVRDFFRGEGLVSSLATRLCGSGDIFSDGRGPAFSFNFIARNFGLPLVD 661
RWAE+N KF +D+++FFRGEGLVSSLATRLCGSGD+FSDGRGPAFSFNFIARN GLPLVD
Sbjct: 619 RWAELNSKFSNDMKEFFRGEGLVSSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVD 678
Query: 662 LVSFSSSKLASELSWNCGEEGPTSNIEVLEKRLKLIRNFIFVLFVSLGVPVLNMGDECGQ 721
LVSFSSSKLASELSWNCGEEGPTSNIEVLEKRLK IRNFIFVLFVSLGVPVLNMGDECGQ
Sbjct: 679 LVSFSSSKLASELSWNCGEEGPTSNIEVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQ 738
Query: 722 SSGGSIAFKDRRPFDWDLLKTGFGTQTTQFITFFSSFRARRFDLFQSRNFLKGENIDWYG 781
SSGGS+AFKDR+ F+WD LKT FGTQTT+FI FFSSFR+RRFDLFQSR+FLKGENIDW+
Sbjct: 739 SSGGSVAFKDRKSFNWDSLKTDFGTQTTRFIAFFSSFRSRRFDLFQSRHFLKGENIDWFD 798
Query: 782 NNLSPPQWEDPSCKFLAVMLRADKEENGLSAENPEPQSDIFMAFNASDQSINVALPEPSE 841
NN SPP+WEDPSCKFLAVMLRADKEE+G ENP+PQSDIFMAFNASDQS++VALPEPSE
Sbjct: 799 NNQSPPEWEDPSCKFLAVMLRADKEESGSITENPKPQSDIFMAFNASDQSVSVALPEPSE 858
Query: 842 GTSWFRLIDTALSFPGFFSSDGELVPTVESVTYEMQAHSCALFEAKSYSD 892
GT+WFRL+DTALSFPGFFSSDGE VPT VTYEMQAHSC LFEAKS++D
Sbjct: 859 GTNWFRLVDTALSFPGFFSSDGEPVPTTSPVTYEMQAHSCTLFEAKSFND 907
BLAST of Moc09g07920 vs. NCBI nr
Match:
XP_004136188.1 (isoamylase 2, chloroplastic [Cucumis sativus] >KGN44865.1 hypothetical protein Csa_016566 [Cucumis sativus])
HSP 1 Score: 1469.1 bits (3802), Expect = 0.0e+00
Identity = 713/885 (80.56%), Postives = 784/885 (88.59%), Query Frame = 0
Query: 7 MATFPSSLVVQPYNVHNFGVTEAFRLAASDHHTHGHMIKHQSGKRDEVRMLAHGESRAGF 66
MATF SSLV+QP+ V+N GVTE+ +LAASDH T+G K+Q GK DE RMLAHGE++ G
Sbjct: 1 MATFLSSLVIQPFCVYNCGVTESPKLAASDHLTYGQKTKYQFGKMDEARMLAHGENKVGA 60
Query: 67 AKSSRYNLSKAYASFGISVEKNGWRFGISGKSKEQEKVATYLFRTECGDLVNVLVRKTGS 126
KSS NLSK YA GISV K+G R GI GKSKEQ +VATYLFRTE GDLVNV V K GS
Sbjct: 61 VKSSHRNLSKTYAKSGISVGKSGQRLGIGGKSKEQRRVATYLFRTEFGDLVNVFVGKKGS 120
Query: 127 GFMVSIELSSVQLANIEETPMLSWGVYRSDSALVTPNIQSSLPDDTIGTIETPFVNTSEG 186
F V+IE+ S+QL +I+E +LSWGVYRSDSALVTPN +SS PD+T G ETPFV TSEG
Sbjct: 121 TFTVNIEVPSMQLVSIDEALLLSWGVYRSDSALVTPNFESSPPDETTGATETPFVKTSEG 180
Query: 187 KFAVELEFDAKHVPFYLSFLLKHPVGVDSGSSEIRSHRKTSFCVPVGFGRGYPSPLGVSI 246
KF+VELEFDAKH PFYLSF+LK+P+GVDSGSSEIRSH+KTSF VPVGFGRGYPSPLG+SI
Sbjct: 181 KFSVELEFDAKHTPFYLSFVLKYPMGVDSGSSEIRSHKKTSFSVPVGFGRGYPSPLGLSI 240
Query: 247 SGDGSMNFSIFSSSAESVVLCLYNDSTSEKPVIELDLDRYINRSGNIWHVSFESVSNFVS 306
SGDGS+NFSIFSSSAES+VLCLYNDSTSEKP++ELDLD YINRSGNIWH SFE S FVS
Sbjct: 241 SGDGSVNFSIFSSSAESLVLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGASKFVS 300
Query: 307 YGYLCKGSKSHKKQDGVEISRILVDPYAKVLTTSSHKTHGHGLGLPSKLLGQICKVPAFD 366
YGY CKGSKSH+ QDG+E+SRI+VDPYAK+L S K+ G GLGLPSK LGQI KVP FD
Sbjct: 301 YGYQCKGSKSHENQDGLEVSRIVVDPYAKILAPSIPKSSGQGLGLPSKFLGQISKVPTFD 360
Query: 367 WDGEVRPNLAMEKLFTYRLNVKRFTVDKSSQLTTDIAGTFAGLTKKLLHFKNLGVNAVLL 426
WDGEV PNL MEKLF YRLNV+RFT+DKSSQL DIAGTF+GLTKKLLHFKNLGVNAVLL
Sbjct: 361 WDGEVHPNLPMEKLFVYRLNVERFTMDKSSQLPADIAGTFSGLTKKLLHFKNLGVNAVLL 420
Query: 427 EPIFPFDEKEGPYFPSHFFSPTNIYGPSGGSISAINSMKEMVKELHANGIEVMLEVVFTN 486
EPIF FDEKEGPYFP HFFSPTN YGPSG SISAINSMKEMVKELHANG+EV+LEVV+T+
Sbjct: 421 EPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVILEVVYTH 480
Query: 487 TAEDGTLQGIDDSSYYFTNRVANLEGKNALNCNYPVVQQLLLDSLRYWVTEFHIDGFCFI 546
T+ +G LQGIDDSSYYFTNRVANLE K+ALNCNYP+VQQLLLDSLRYWVTEFH+DGFCF+
Sbjct: 481 TSGNGALQGIDDSSYYFTNRVANLEEKSALNCNYPIVQQLLLDSLRYWVTEFHVDGFCFV 540
Query: 547 NASSLLRGYRGELLSRPPLVEAIAFDPLLSKTKIIADFWDPHESISKETRFPHWKRWAEV 606
NAS LLRG+ GELLSRPP VEAIAFDPLLSKTK++ADFWDP E SKETRFPHWKRWAEV
Sbjct: 541 NASFLLRGHHGELLSRPPFVEAIAFDPLLSKTKLVADFWDPQELESKETRFPHWKRWAEV 600
Query: 607 NYKFCSDVRDFFRGEGLVSSLATRLCGSGDIFSDGRGPAFSFNFIARNFGLPLVDLVSFS 666
N KFCSD+RDFFRGEGL+SSLATRLCGSGD+FSDGRGPAFSFNFIARN GLPLVDLVSFS
Sbjct: 601 NSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS 660
Query: 667 SSKLASELSWNCGEEGPTSNIEVLEKRLKLIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 726
+S LASELSWNCGEEGPTSN++VLEKRLK IRNFIFVLFVSLGVPVLNMGDECGQSSGGS
Sbjct: 661 NSNLASELSWNCGEEGPTSNLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 720
Query: 727 IAFKDRRPFDWDLLKTGFGTQTTQFITFFSSFRARRFDLFQSRNFLKGENIDWYGNNLSP 786
+AF D+R F+WDLLKT FGTQTTQFI F SSFR+RRFDLFQ+RNFLKGENIDW+ NN SP
Sbjct: 721 VAFNDKRSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLFQNRNFLKGENIDWFDNNQSP 780
Query: 787 PQWEDPSCKFLAVMLRADKEENGLSAENPEPQSDIFMAFNASDQSINVALPEPSEGTSWF 846
PQWED SCKFLAVMLRADKEEN ENP+ +S+IFM FNASDQS +VALPEP EGTSWF
Sbjct: 781 PQWEDASCKFLAVMLRADKEENESITENPKTRSNIFMVFNASDQSESVALPEPLEGTSWF 840
Query: 847 RLIDTALSFPGFFSSDGELVPTVESVTYEMQAHSCALFEAKSYSD 892
R++DTAL FPGFFSSDGELVP SVTYE+QAHSCALFEAKS +D
Sbjct: 841 RVVDTALPFPGFFSSDGELVPMTGSVTYEIQAHSCALFEAKSAND 885
BLAST of Moc09g07920 vs. NCBI nr
Match:
KAA0057598.1 (isoamylase 2 [Cucumis melo var. makuwa] >TYK20996.1 isoamylase 2 [Cucumis melo var. makuwa])
HSP 1 Score: 1468.4 bits (3800), Expect = 0.0e+00
Identity = 714/882 (80.95%), Postives = 778/882 (88.21%), Query Frame = 0
Query: 7 MATFPSSLVVQPYNVHNFGVTEAFRLAASDHHTHGHMIKHQSGKRDEVRMLAHGESRAGF 66
MATF SSLV+QP+ VHN GVTE+ +LA SDH T+G K+QSGK DE RMLAHGES+ G
Sbjct: 1 MATFLSSLVIQPFCVHNCGVTESLKLAVSDHLTYGQKTKYQSGKMDEARMLAHGESKVGA 60
Query: 67 AKSSRYNLSKAYASFGISVEKNGWRFGISGKSKEQEKVATYLFRTECGDLVNVLVRKTGS 126
KSS NLSKAY + GISV K+G R GI GKSKEQ KVATYLFRTE GDLVNV V KTGS
Sbjct: 61 VKSSHRNLSKAYVTSGISVGKSGQRLGIGGKSKEQRKVATYLFRTEFGDLVNVFVGKTGS 120
Query: 127 GFMVSIELSSVQLANIEETPMLSWGVYRSDSALVTPNIQSSLPDDTIGTIETPFVNTSEG 186
F V+IE+SS+QL + +ET +LSW VYRSDSA VT N QSS PD T G ETPFV TSEG
Sbjct: 121 AFTVNIEISSMQLVSTDETLLLSWRVYRSDSAPVTSNFQSSPPDGTTGATETPFVKTSEG 180
Query: 187 KFAVELEFDAKHVPFYLSFLLKHPVGVDSGSSEIRSHRKTSFCVPVGFGRGYPSPLGVSI 246
KF+VELEFDAKH PFYLSF+LK P+GVDSGSSEIRSHRKTSF VPVGFGRGYPSP G+SI
Sbjct: 181 KFSVELEFDAKHTPFYLSFVLKFPMGVDSGSSEIRSHRKTSFSVPVGFGRGYPSPSGLSI 240
Query: 247 SGDGSMNFSIFSSSAESVVLCLYNDSTSEKPVIELDLDRYINRSGNIWHVSFESVSNFVS 306
SGDGS+NFSIFSS+AESVVLCLYNDSTSEKP++ELDLD YINRSGNIWH SFE VSNFVS
Sbjct: 241 SGDGSINFSIFSSTAESVVLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGVSNFVS 300
Query: 307 YGYLCKGSKSHKKQDGVEISRILVDPYAKVLTTSSHKTHGHGLGLPSKLLGQICKVPAFD 366
YGY CKG KSH+ QDG+E+SRI+VDPYAK+L S K+ GLG P K LGQI VPAFD
Sbjct: 301 YGYQCKGLKSHENQDGLEVSRIVVDPYAKILAPSIPKSRDQGLGFPLKFLGQISIVPAFD 360
Query: 367 WDGEVRPNLAMEKLFTYRLNVKRFTVDKSSQLTTDIAGTFAGLTKKLLHFKNLGVNAVLL 426
WDGEVRPNL MEKLF YRLNVKRFT+DKSSQL DIAGTF+GLTKKLLH KNLGVNAVLL
Sbjct: 361 WDGEVRPNLPMEKLFVYRLNVKRFTIDKSSQLPADIAGTFSGLTKKLLHLKNLGVNAVLL 420
Query: 427 EPIFPFDEKEGPYFPSHFFSPTNIYGPSGGSISAINSMKEMVKELHANGIEVMLEVVFTN 486
EPIF FDEKEGPYFP HFFSPTN YGPSG SISAINSMKEMVKELHANG+EV++EVV+T+
Sbjct: 421 EPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVIVEVVYTH 480
Query: 487 TAEDGTLQGIDDSSYYFTNRVANLEGKNALNCNYPVVQQLLLDSLRYWVTEFHIDGFCFI 546
T+ DG LQGIDDSSYYF NRVANLE ++ALNCNYP+VQQLLLDSLRYWVTEFH+DGFCF+
Sbjct: 481 TSVDGALQGIDDSSYYFANRVANLEERSALNCNYPIVQQLLLDSLRYWVTEFHVDGFCFV 540
Query: 547 NASSLLRGYRGELLSRPPLVEAIAFDPLLSKTKIIADFWDPHESISKETRFPHWKRWAEV 606
NAS LL+G+ GELLSRPP VEAIAFDP+LSKTK++ADFWDP E +SKETRFPHWKRWAEV
Sbjct: 541 NASFLLQGHHGELLSRPPFVEAIAFDPILSKTKLVADFWDPQELVSKETRFPHWKRWAEV 600
Query: 607 NYKFCSDVRDFFRGEGLVSSLATRLCGSGDIFSDGRGPAFSFNFIARNFGLPLVDLVSFS 666
N KFCSD+RDFFRGEGL+SSLATRLCGSGD+FSDGRGPAFSFNFIARN GLPLVDLVSFS
Sbjct: 601 NSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS 660
Query: 667 SSKLASELSWNCGEEGPTSNIEVLEKRLKLIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 726
+S LASELSWNCGEEGPT+ ++VLEKRLK IRNFIFVLFVSLGVPVLNMGDECGQSSGGS
Sbjct: 661 NSNLASELSWNCGEEGPTNTLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 720
Query: 727 IAFKDRRPFDWDLLKTGFGTQTTQFITFFSSFRARRFDLFQSRNFLKGENIDWYGNNLSP 786
+AF DRR F+WDLLKT FGTQTTQFI F SSFR+RRFDLFQSRNFLKGENIDW+ NN SP
Sbjct: 721 VAFNDRRSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLFQSRNFLKGENIDWFDNNQSP 780
Query: 787 PQWEDPSCKFLAVMLRADKEENGLSAENPEPQSDIFMAFNASDQSINVALPEPSEGTSWF 846
PQWED SCKFLAVMLRADKEEN ENP+ +SDIFMAFNASDQS +VALPEPSEGTSWF
Sbjct: 781 PQWEDASCKFLAVMLRADKEENESITENPKTRSDIFMAFNASDQSESVALPEPSEGTSWF 840
Query: 847 RLIDTALSFPGFFSSDGELVPTVESVTYEMQAHSCALFEAKS 889
R++DTAL FPGFFSSDGELVP SVTYEMQAHSCALFEAKS
Sbjct: 841 RVVDTALPFPGFFSSDGELVPMTGSVTYEMQAHSCALFEAKS 882
BLAST of Moc09g07920 vs. ExPASy Swiss-Prot
Match:
Q8L735 (Isoamylase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ISA2 PE=1 SV=2)
HSP 1 Score: 924.5 bits (2388), Expect = 9.3e-268
Identity = 459/824 (55.70%), Postives = 595/824 (72.21%), Query Frame = 0
Query: 72 YNLSKAYASFGISVEKNGWRFGISGKSKEQEKVATYLFRTECGDLVNVLVRKTGSGFMVS 131
Y SK AS S+E ++ + +KV +Y FRT+ G LV V V K + +
Sbjct: 64 YQFSKICAS-KTSIELREALSSRRAEADDLKKVTSYSFRTKAGALVKVKVEKKREKYSIL 123
Query: 132 IELSSVQLANIEETPM-LSWGVYRSDSALVTP-NIQSSLPDDTIGTIETPFVNTSEGKFA 191
+ +SS++L+ +++ + + WGVYRSDS+ P + ++S D T ET FV +S +
Sbjct: 124 VYVSSLELSGDDKSRLVMVWGVYRSDSSCFLPLDFENSSQDSQTHTTETTFVKSSLSELM 183
Query: 192 VELEFDAKHVPFYLSFLLKHPVGVDSGSSEIRSHRKTSFCVPVGFGRGYPSPLGVSIS-G 251
+ LEFD K PFYLSF LK G D E+ +HR T FC+PVGF G+P PLG+S
Sbjct: 184 LGLEFDGKESPFYLSFHLKLVSGRDPDGQEMLTHRDTDFCIPVGFTAGHPLPLGLSSGPD 243
Query: 252 DGSMNFSIFSSSAESVVLCLYNDSTSEKPVIELDLDRYINRSGNIWHVSFESVSNFVSYG 311
D S NFS FS S+ +VVLCLY+DST++KP +ELDLD Y+NR+G++WH S ++ +FV YG
Sbjct: 244 DDSWNFSFFSRSSTNVVLCLYDDSTTDKPALELDLDPYVNRTGDVWHASVDNTWDFVRYG 303
Query: 312 YLCKGSKSHKKQDGVEISRILVDPYAKVLTTSSHKTHGHGLGLPSKLLGQICKVPAFDWD 371
Y CK + K+ VE I++DPYA V+ G + K LG + K P+FDW
Sbjct: 304 YRCKETAHSKEDVDVEGEPIVLDPYATVV----------GKSVSQKYLGSLSKSPSFDWG 363
Query: 372 GEVRPNLAMEKLFTYRLNVKRFTVDKSSQLTTDIAGTFAGLTKKLLHFKNLGVNAVLLEP 431
+V PN+ +EKL YRLNVK FT +SS+L +++AGTF+G+ +K+ H K LG NAVLLEP
Sbjct: 364 EDVSPNIPLEKLLVYRLNVKGFTQHRSSKLPSNVAGTFSGVAEKVSHLKTLGTNAVLLEP 423
Query: 432 IFPFDEKEGPYFPSHFFSPTNIYGPSGGSISAINSMKEMVKELHANGIEVMLEVVFTNTA 491
IF F E++GPYFP HFFSP +IYGPS SA+NSMK MVK+LH+ GIEV+LEVVFT+TA
Sbjct: 424 IFSFSEQKGPYFPFHFFSPMDIYGPSNSLESAVNSMKVMVKKLHSEGIEVLLEVVFTHTA 483
Query: 492 EDGTLQGIDDSSYYFTNRVANLEGKNALNCNYPVVQQLLLDSLRYWVTEFHIDGFCFINA 551
+ G L+GIDDSSYY+ R +L+ K+ LNCNYPVVQQL+L+SLRYWVTEFH+DGFCFINA
Sbjct: 484 DSGALRGIDDSSYYYKGRANDLDSKSYLNCNYPVVQQLVLESLRYWVTEFHVDGFCFINA 543
Query: 552 SSLLRGYRGELLSRPPLVEAIAFDPLLSKTKIIADFWDPHESISKETRFPHWKRWAEVNY 611
SSLLRG GE LSRPPLVEAIAFDPLL++TK+IAD WDP E + KE RFPHWKRWAE+N
Sbjct: 544 SSLLRGVHGEQLSRPPLVEAIAFDPLLAETKLIADCWDPLEMMPKEVRFPHWKRWAELNT 603
Query: 612 KFCSDVRDFFRGEGLVSSLATRLCGSGDIFSDGRGPAFSFNFIARNFGLPLVDLVSFSSS 671
++C +VR+F RG G++S LATR+CGSGD+F+DGRGPAFSFN+I+RN GL LVD+VSFS
Sbjct: 604 RYCRNVRNFLRGRGVLSDLATRICGSGDVFTDGRGPAFSFNYISRNSGLSLVDIVSFSGP 663
Query: 672 KLASELSWNCGEEGPTSNIEVLEKRLKLIRNFIFVLFVSLGVPVLNMGDECGQSSGGSIA 731
+LASELSWNCGEEG T+ VL++RLK IRNF+F+ ++SLGVPVLNMGDECG S+ GS
Sbjct: 664 ELASELSWNCGEEGATNKSAVLQRRLKQIRNFLFIQYISLGVPVLNMGDECGISTRGSPL 723
Query: 732 FKDRRPFDWDLLKTGFGTQTTQFITFFSSFRARRFDLFQSRNFLKGENIDWYGNNLSPPQ 791
+ R+PFDW+LL + FGTQ TQFI+F +S RARR D+FQ R+FLK ENI WY N+ + P+
Sbjct: 724 LESRKPFDWNLLASAFGTQITQFISFMTSVRARRSDVFQRRDFLKPENIVWYANDQTTPK 783
Query: 792 WEDPSCKFLAVMLRADKEENGLS--AENPEPQS-DIFMAFNASDQSINVALPEPSEGTSW 851
WEDP+ KFLA+ ++++ EE + AE EP+S D+F+ FNASD +V LP +G+ W
Sbjct: 784 WEDPASKFLALEIKSESEEEETASLAEPNEPKSNDLFIGFNASDHPESVVLPSLPDGSKW 843
Query: 852 FRLIDTALSFPGFFSSDGELV----PTVESVTYEMQAHSCALFE 886
RL+DTAL FPGFFS +GE V P + V YEM+ +SC LFE
Sbjct: 844 RRLVDTALPFPGFFSVEGETVVAEEPLQQLVVYEMKPYSCTLFE 876
BLAST of Moc09g07920 vs. ExPASy Swiss-Prot
Match:
Q6AU80 (Isoamylase 2, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=ISA2 PE=1 SV=1)
HSP 1 Score: 769.6 bits (1986), Expect = 3.8e-221
Identity = 383/717 (53.42%), Postives = 510/717 (71.13%), Query Frame = 0
Query: 189 AVELEFDAKHVPFYLSFLLKHPVGVDSGSSEIRSHRKTSFCVPVGFGRGYPSPLGVSISG 248
A EL FD PFYLSFLL D+ +EIR+HRKTSF VPVG G G P+PLG+SISG
Sbjct: 105 AAELSFDPTRAPFYLSFLL-----TDASGAEIRTHRKTSFRVPVGVGPGSPAPLGMSISG 164
Query: 249 DGSMNFSIFSSSAESVVLCLY----NDSTSEKPVIELDLDRYINRSGNIWHVSFESVSNF 308
DG++NF+++S +A +V L LY ++P +E+DLD YI+R+GN+WHVS SV +
Sbjct: 165 DGAVNFAVYSKNANAVSLYLYAAAVGGGGGDEPALEIDLDPYIHRTGNVWHVSLASVDGY 224
Query: 309 VSYGYLCKGSKSHKKQDGVEISRILVDPYAKVL--TTSSHKTHGHGLGLPS-KLLGQICK 368
VSY + C G I R L+DPYAKV+ SS+ + G+ PS + +
Sbjct: 225 VSYAFCCGG-----------IRRPLLDPYAKVIGDFVSSNSVYDEGVTAPSMRCFASLAI 284
Query: 369 VPAFDWDGEVRPNLAMEKLFTYRLNVKRFTVDKSSQLTTDIAGTFAGLTKKLLHFKNLGV 428
P+++W + P L +EKL YR NV FT D+SS L D AGTF GL+ K+ HF++LGV
Sbjct: 285 APSYNWGRDRHPRLPLEKLVVYRANVALFTKDRSSGLPDDAAGTFTGLSAKVEHFRSLGV 344
Query: 429 NAVLLEPIFPFDEKEGPYFPSHFFSPTNIYGPSGGSISAINSMKEMVKELHANGIEVMLE 488
NA+LLEP+FPF + +GPYFP HFFSP N+Y G S+SAI SMK+MV+ +H NGIEV+LE
Sbjct: 345 NAILLEPVFPFHQVKGPYFPYHFFSPMNLYSSKGLSVSAIKSMKDMVRVMHRNGIEVLLE 404
Query: 489 VVFTNTAEDG------TLQGIDDSSYYFTNRVANLEGKNALNCNYPVVQQLLLDSLRYWV 548
VVFT+TAE +++GID+SSYY N +A + + LNCN+PV Q+L+LDSLR+WV
Sbjct: 405 VVFTHTAEGESECQTISMRGIDNSSYYIANGIAGCKA-SILNCNHPVTQKLILDSLRHWV 464
Query: 549 TEFHIDGFCFINASSLLRGYRGELLSRPPLVEAIAFDPLLSKTKIIADFWDPHESISKET 608
+FH+DGFCFINA L+RG GE LSRPPL+EAI FDP+LS TKIIAD W P + + +
Sbjct: 465 LDFHVDGFCFINAPFLVRGPGGEYLSRPPLLEAITFDPVLSMTKIIADPWSPLDISNVQF 524
Query: 609 RFPHWKRWAEVNYKFCSDVRDFFRGEGLVSSLATRLCGSGDIFSDGRGPAFSFNFIARNF 668
FPHWKRWAEVN +F DVR F + E L+S LATRLCGSGD+FS RGPAFSFN ++RN
Sbjct: 525 PFPHWKRWAEVNTRFSIDVRKFLKREALISDLATRLCGSGDLFST-RGPAFSFNHVSRNS 584
Query: 669 GLPLVDLVSFSSSKLASELSWNCGEEGPTSNIEVLEKRLKLIRNFIFVLFVSLGVPVLNM 728
GL LVDLVSFS+ L SE SWNCGEEGP+ N VL+ RL+ IRNF+F+LFVSLGVPVLNM
Sbjct: 585 GLSLVDLVSFSNDDLLSESSWNCGEEGPSENSAVLQTRLRQIRNFLFILFVSLGVPVLNM 644
Query: 729 GDECGQSSGGSIAFKDRRPFDWDLLKTGFGTQTTQFITFFSSFRARRFDLFQSRNFLKGE 788
GDECG S+ GS+++KDR P +W +KT F + T FI+F ++ R+RR D+FQ R FLK E
Sbjct: 645 GDECGHSAAGSVSYKDRGPLNWRGMKTTFVKEVTGFISFLTALRSRRGDIFQRREFLKLE 704
Query: 789 NIDWYGNNLSPPQWEDPSCKFLAVMLRADKEENGLSAENPEPQSDIFMAFNASDQSINVA 848
NI WYG++L P W+DP+ FL + + A+ +E ++A++ + D+++ FNA+++S++ A
Sbjct: 705 NIHWYGSDLCEPGWDDPTSNFLCMHINAEVDE--MAADS--VRGDLYICFNANEESVSAA 764
Query: 849 LPEPSEGTSWFRLIDTALSFPGFFSSDGELVPTVESV----TYEMQAHSCALFEAKS 889
LP +EG+ W RL+DT+L+FPGFF+++ P V+ V +Y ++AH+C LFE+KS
Sbjct: 765 LPALAEGSVWLRLVDTSLAFPGFFATESN--PKVQQVPGLSSYHVEAHTCVLFESKS 797
BLAST of Moc09g07920 vs. ExPASy Swiss-Prot
Match:
Q9M0S5 (Isoamylase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ISA3 PE=1 SV=2)
HSP 1 Score: 375.2 bits (962), Expect = 2.1e-102
Identity = 254/704 (36.08%), Postives = 363/704 (51.56%), Query Frame = 0
Query: 237 GYPSPLGVSISGDGSMNFSIFSSSAESVVLCLY-----NDSTSEKPVIELDLDRYINRSG 296
G SPLGVS D +NF++FS +A SV LCL D T + +IEL LD +N++G
Sbjct: 88 GEVSPLGVS-QVDKGINFALFSQNATSVTLCLSLSQSGKDDTDDDGMIELVLDPSVNKTG 147
Query: 297 NIWHVSFESVS-NFVSYGYLCKGSKSHKKQDGVEISRILVDPYAKVLTTSSHKTHGHGLG 356
+ WH+ E + N V YGY G ++ + S +L+DPYAK++ H + G
Sbjct: 148 DTWHICVEDLPLNNVLYGYRVDGPGEWQQGHRFDRSILLLDPYAKLV--KGHSSFGDSSQ 207
Query: 357 LPSKLLGQI-CKVPAFDWDGEVR-PNLAMEKLFTYRLNVKRFTVDKSSQLTTDIAGTFAG 416
++ G + FDW + + PN+ + L Y +NV+ FT D+SS + I G++ G
Sbjct: 208 KFAQFYGTYDFESSPFDWGDDYKFPNIPEKDLVIYEMNVRAFTADESSGMDPAIGGSYLG 267
Query: 417 LTKKLLHFKNLGVNAVLLEPIFPFDEKE--------------GPYFPSHFFSPTNIYGP- 476
+K+ H ++LG+NAV L P+F FDE E Y +FF+P + Y
Sbjct: 268 FIEKIPHLQDLGINAVELLPVFEFDELELQRRSNPRDHMVNTWGYSTVNFFAPMSRYASG 327
Query: 477 SGGSISAINSMKEMVKELHANGIEVMLEVVF--TNTAED-----GTLQGIDDSSYYF--- 536
G I A KEMVK LH+ GIEV+L+VV+ TN A+D + +GID+ YY
Sbjct: 328 EGDPIKASKEFKEMVKALHSAGIEVILDVVYNHTNEADDKYPYTTSFRGIDNKVYYMLDP 387
Query: 537 TNRVANLEG-KNALNCNYPVVQQLLLDSLRYWVTEFHIDGFCFINASSLLRGYRGELLSR 596
N++ N G N LNCN+PVV +L+LDSLR+WVTE+H+DGF F AS L R G LS
Sbjct: 388 NNQLLNFSGCGNTLNCNHPVVMELILDSLRHWVTEYHVDGFRFDLASVLCRATDGSPLSA 447
Query: 597 PPLVEAIAFDPLLSKTKIIADFWDPHESISKETRFPHWKRWAEVNYKFCSDVRDFFRGE- 656
PPL+ AIA D +LS+ KIIA+ WD + +FP+W RWAE N + DVR F +G+
Sbjct: 448 PPLIRAIAKDSVLSRCKIIAEPWD-CGGLYLVGKFPNWDRWAEWNGMYRDDVRRFIKGDS 507
Query: 657 GLVSSLATRLCGSGDIFS-DGRGPAFSFNFIARNFGLPLVDLVSFS-----------SSK 716
G+ S ATR+ GS D++ + R P NF+ + G L DLVS++ +
Sbjct: 508 GMKGSFATRVSGSSDLYQVNQRKPYHGVNFVIAHDGFTLRDLVSYNFKHNEANGEGGNDG 567
Query: 717 LASELSWNCGEEGPTSNIEVLEKRLKLIRNFIFVLFVSLGVPVLNMGDECGQSS-GGSIA 776
SWNCG EG T + + R + ++NF L +S G P++ MGDE G + G + +
Sbjct: 568 CNDNHSWNCGFEGETGDAHIKSLRTRQMKNFHLALMISQGTPMMLMGDEYGHTRYGNNNS 627
Query: 777 F---KDRRPFDWDLLKTGFGTQTTQFITFFSSFRARRFD--LFQSRNFLKGENIDWYGNN 836
+ F W L + FFS R + + NFL I W+ +N
Sbjct: 628 YGHDTSLNNFQWKEL----DAKKQNHFRFFSEVIKFRHSHHVLKHENFLTQGEITWHEDN 687
Query: 837 LSPPQWEDPSCKFLAVMLRADKEENGLSAENPEPQSDIFMAFNASDQSINVALPEPSEGT 888
W++ KFLA L +G+ DI++AFNA D + +P+P G
Sbjct: 688 -----WDNSESKFLAFTL-----HDGIGGR------DIYVAFNAHDYFVKALIPQPPPGK 747
BLAST of Moc09g07920 vs. ExPASy Swiss-Prot
Match:
B9G434 (Isoamylase 3, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=ISA3 PE=2 SV=1)
HSP 1 Score: 369.0 bits (946), Expect = 1.5e-100
Identity = 254/714 (35.57%), Postives = 366/714 (51.26%), Query Frame = 0
Query: 230 VPVGFGRGYPSPLGVSISGDGSMNFSIFSSSAESVVLCLY-----NDSTSEKPVIELDLD 289
+P + G PLGVS +G +NF++FS A SV+LCL + V+E LD
Sbjct: 96 MPFKYSSGKAFPLGVS-QVEGGLNFALFSQHASSVILCLKLPGRGTEDEKGADVVEFVLD 155
Query: 290 RYINRSGNIWHVSFESV-SNFVSYGYLCKGSKSHKKQDGVEISRILVDPYAKVLTTSSHK 349
+ N++G+IWHV E + ++ V YGY G + + + S +L+DPYAK++ S K
Sbjct: 156 QQKNKTGDIWHVIVEGLPASGVLYGYRVGGPQGWDQGHRFDSSTVLLDPYAKLV--SGRK 215
Query: 350 THGHGLGLPSKLLGQI-CKVPAFDWDGEVR-PNLAMEKLFTYRLNVKRFTVDKSSQLTTD 409
G S+ G FDW + R PNL L Y +NV+ FT D+SS L +
Sbjct: 216 YFGVAEEKSSQHFGTYDFDSSPFDWGDDYRLPNLPEADLVIYEMNVRAFTADESSGLDST 275
Query: 410 IAGTFAGLTKKLLHFKNLGVNAVLLEPIFPFDEKEGPYFPS--------------HFFSP 469
G++ GL K+ H LGVNAV L P+F +DE E +P+ +FF+P
Sbjct: 276 SRGSYLGLIDKIPHLLELGVNAVELLPVFEYDELEFKRYPNPRDHMVNTWGYSTINFFAP 335
Query: 470 TNIYGPSGGS-ISAINSMKEMVKELHANGIEVMLEVVF--TNTAEDG-----TLQGIDDS 529
+ Y +GG ++A +K+MVKELH GIEV+L+VV+ TN A+D + +GID+
Sbjct: 336 MSRYASAGGGPVAASKELKQMVKELHKAGIEVILDVVYNHTNEADDAHPYMTSFRGIDNK 395
Query: 530 SYYFTNRVANLE------GKNALNCNYPVVQQLLLDSLRYWVTEFHIDGFCFINASSLLR 589
YY + N E N LNCN+PVV++L+LDSLR+WV E+HIDGF F AS L R
Sbjct: 396 VYYMLDLNKNAELLNFSGCGNTLNCNHPVVKELILDSLRHWVEEYHIDGFRFDLASVLCR 455
Query: 590 GYRGELLSRPPLVEAIAFDPLLSKTKIIADFWDPHESISKETRFPHWKRWAEVNYKFCSD 649
G G L PPL++ IA D +LS+ KIIA+ WD + RFP+W RWAE N K+ D
Sbjct: 456 GPDGCPLDAPPLIKEIAKDAVLSRCKIIAEPWD-CGGLYLVGRFPNWDRWAEWNGKYRDD 515
Query: 650 VRDFFRGE-GLVSSLATRLCGSGDIFS-DGRGPAFSFNFIARNFGLPLVDLVSFS----- 709
+R F +G+ G+ ATR+ GS D++ + R P NF+ + G L DLVS++
Sbjct: 516 LRRFIKGDPGMKGVFATRVSGSADLYQVNERKPYHGVNFVIAHDGFTLCDLVSYNLKHND 575
Query: 710 ------SSKLASELSWNCGEEGPTSNIEVLEKRLKLIRNFIFVLFVSLGVPVLNMGDECG 769
SWNCG EG T+++ VL R + ++NF L +S G P++ MGDE G
Sbjct: 576 ANGEGGCDGCNDNFSWNCGVEGETNDLNVLSLRSRQMKNFHVALMISQGTPMMLMGDEYG 635
Query: 770 QSSGGSIAFKDR----RPFDWDLL---KTGFGTQTTQFITFFSSFRARRFDLFQSRNFLK 829
+ G+ F W+ L + G ++ I F S R D F ++N
Sbjct: 636 HTRYGNNNSYGHDTCINNFQWEQLEQRRDGHFRFFSEMIKFRHSNPILRRDRFLNKN--- 695
Query: 830 GENIDWYGNNLSPPQWEDPSCKFLAVMLRADKEENGLSAENPEPQSDIFMAFNASDQSIN 888
++ W+ + WE+ KFLA + D G DI++AFNA D ++
Sbjct: 696 --DVTWHED-----CWENQESKFLAFTVH-DHNSGG----------DIYLAFNAHDYFVD 755
BLAST of Moc09g07920 vs. ExPASy Swiss-Prot
Match:
D0TZF0 (Isoamylase 1, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=ISA1 PE=1 SV=1)
HSP 1 Score: 341.3 bits (874), Expect = 3.4e-92
Identity = 233/690 (33.77%), Postives = 342/690 (49.57%), Query Frame = 0
Query: 237 GYPSPLGVSISGDGSMNFSIFSSSAESVVLCLY--NDSTSEKPVIELDLDRYINRSGNIW 296
G P+PLG + + DG +NF+++S+ A + LCL+ +D +++ E+ LD NR+GN+W
Sbjct: 99 GMPAPLGAT-ALDGGVNFAVYSAGASAASLCLFTPDDLEADEVTEEVPLDPLFNRTGNVW 158
Query: 297 HVSFESVSNFVSYGYLCKGSKSHKKQDGVEISRILVDPYAK-VLTTSSHKTHGHGLGLPS 356
HV E + + YGY G + ++S ++VDPYAK V++ + G G
Sbjct: 159 HVFIEGELHNMLYGYRFDGMFAPHCGQYFDVSNVVVDPYAKAVISRGEYGVPGPGGDCWP 218
Query: 357 KLLGQI-CKVPAFDWDGEVRPNLAMEKLFTYRLNVKRFTVDKSSQLTTDIAGTFAGLTKK 416
++ G I FDW G++ + L Y ++++ FT K S + GT+ G K
Sbjct: 219 QMAGMIPLPYSTFDWQGDLPLRYPQKDLVIYEMHLRGFT--KHSSSNVEHPGTYIGAISK 278
Query: 417 LLHFKNLGVNAVLLEPIFPFDEKE----------GPYFPSHFFSPTNIYGPSGG----SI 476
L + K LGVN V L P F+E E Y +FFSP Y SGG
Sbjct: 279 LDYLKELGVNCVELMPCHEFNELEYFSCSSKMNFWGYSTINFFSPMIRYS-SGGIRNCGR 338
Query: 477 SAINSMKEMVKELHANGIEVMLEVVFTNTAEDG------TLQGIDDSSYYF---TNRVAN 536
AIN K V+E H GIEV+++VVF +TAE + +GID+S+YY N
Sbjct: 339 DAINEFKTFVREAHKRGIEVIMDVVFNHTAEGNEKGPILSFRGIDNSTYYMLAPKGEFYN 398
Query: 537 LEG-KNALNCNYPVVQQLLLDSLRYWVTEFHIDGFCFINASSLLRG-------------- 596
G N NCN+PVV++ ++D LRYWVTE H+DGF F AS + RG
Sbjct: 399 YSGCGNTFNCNHPVVREFIVDCLRYWVTEMHVDGFRFDLASIMTRGCSLWDPVNVYGSPV 458
Query: 597 -----YRGELLSRPPLVEAIAFDPLLSKTKIIADFWDPHESISKETRFPHWKRWAEVNYK 656
G L+ PPL++ I+ DP+L K+IA+ WD + + +FPHWK W+E N K
Sbjct: 459 EGDMTTTGTPLATPPLIDMISNDPILGDVKLIAEAWDA-GGLYQVGQFPHWKIWSEWNGK 518
Query: 657 FCSDVRDFFRG-EGLVSSLATRLCGSGDIF-SDGRGPAFSFNFIARNFGLPLVDLVSFSS 716
+ VR F +G +G A LCGS ++ + GR P S NF+ + G L DLV+++
Sbjct: 519 YRDIVRQFIKGTDGFAGGFAECLCGSPHLYQAGGRKPWHSINFVCAHDGFTLADLVTYNK 578
Query: 717 SKLAS-----------ELSWNCGEEGPTSNIEVLEKRLKLIRNFIFVLFVSLGVPVLNMG 776
+S LSWNCGEEG + + V R + +RNF L VS GVP+ MG
Sbjct: 579 KYNSSNGEDNRDGENHNLSWNCGEEGEFAGLSVKRLRKRQMRNFFVSLMVSQGVPMFYMG 638
Query: 777 DECGQSSGG--SIAFKDR--RPFDWDLLKTGFGTQTTQFITFFSSFRARRFDLFQSRNFL 836
DE G + GG + D F WD K + +F + + FR ++ + +F
Sbjct: 639 DEYGHTKGGNNNTYCHDHYVNYFRWD--KKEESSDLQRFCSLMTKFR-KQCESLGLADFP 698
Query: 837 KGENIDWYGNNLSPPQWEDPSCKFLAVMLRADKEENGLSAENPEPQSDIFMAFNASDQSI 863
+ + W+G+ P W + S +F+A + E + +I++AFNAS
Sbjct: 699 TAQRLHWHGHQPGKPDWSETS-RFVAF------------STKDETKGEIYVAFNASHLPA 758
BLAST of Moc09g07920 vs. ExPASy TrEMBL
Match:
A0A6J1DAX4 (isoamylase 2, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111018448 PE=4 SV=1)
HSP 1 Score: 1814.3 bits (4698), Expect = 0.0e+00
Identity = 891/891 (100.00%), Postives = 891/891 (100.00%), Query Frame = 0
Query: 1 MLLQPQMATFPSSLVVQPYNVHNFGVTEAFRLAASDHHTHGHMIKHQSGKRDEVRMLAHG 60
MLLQPQMATFPSSLVVQPYNVHNFGVTEAFRLAASDHHTHGHMIKHQSGKRDEVRMLAHG
Sbjct: 1 MLLQPQMATFPSSLVVQPYNVHNFGVTEAFRLAASDHHTHGHMIKHQSGKRDEVRMLAHG 60
Query: 61 ESRAGFAKSSRYNLSKAYASFGISVEKNGWRFGISGKSKEQEKVATYLFRTECGDLVNVL 120
ESRAGFAKSSRYNLSKAYASFGISVEKNGWRFGISGKSKEQEKVATYLFRTECGDLVNVL
Sbjct: 61 ESRAGFAKSSRYNLSKAYASFGISVEKNGWRFGISGKSKEQEKVATYLFRTECGDLVNVL 120
Query: 121 VRKTGSGFMVSIELSSVQLANIEETPMLSWGVYRSDSALVTPNIQSSLPDDTIGTIETPF 180
VRKTGSGFMVSIELSSVQLANIEETPMLSWGVYRSDSALVTPNIQSSLPDDTIGTIETPF
Sbjct: 121 VRKTGSGFMVSIELSSVQLANIEETPMLSWGVYRSDSALVTPNIQSSLPDDTIGTIETPF 180
Query: 181 VNTSEGKFAVELEFDAKHVPFYLSFLLKHPVGVDSGSSEIRSHRKTSFCVPVGFGRGYPS 240
VNTSEGKFAVELEFDAKHVPFYLSFLLKHPVGVDSGSSEIRSHRKTSFCVPVGFGRGYPS
Sbjct: 181 VNTSEGKFAVELEFDAKHVPFYLSFLLKHPVGVDSGSSEIRSHRKTSFCVPVGFGRGYPS 240
Query: 241 PLGVSISGDGSMNFSIFSSSAESVVLCLYNDSTSEKPVIELDLDRYINRSGNIWHVSFES 300
PLGVSISGDGSMNFSIFSSSAESVVLCLYNDSTSEKPVIELDLDRYINRSGNIWHVSFES
Sbjct: 241 PLGVSISGDGSMNFSIFSSSAESVVLCLYNDSTSEKPVIELDLDRYINRSGNIWHVSFES 300
Query: 301 VSNFVSYGYLCKGSKSHKKQDGVEISRILVDPYAKVLTTSSHKTHGHGLGLPSKLLGQIC 360
VSNFVSYGYLCKGSKSHKKQDGVEISRILVDPYAKVLTTSSHKTHGHGLGLPSKLLGQIC
Sbjct: 301 VSNFVSYGYLCKGSKSHKKQDGVEISRILVDPYAKVLTTSSHKTHGHGLGLPSKLLGQIC 360
Query: 361 KVPAFDWDGEVRPNLAMEKLFTYRLNVKRFTVDKSSQLTTDIAGTFAGLTKKLLHFKNLG 420
KVPAFDWDGEVRPNLAMEKLFTYRLNVKRFTVDKSSQLTTDIAGTFAGLTKKLLHFKNLG
Sbjct: 361 KVPAFDWDGEVRPNLAMEKLFTYRLNVKRFTVDKSSQLTTDIAGTFAGLTKKLLHFKNLG 420
Query: 421 VNAVLLEPIFPFDEKEGPYFPSHFFSPTNIYGPSGGSISAINSMKEMVKELHANGIEVML 480
VNAVLLEPIFPFDEKEGPYFPSHFFSPTNIYGPSGGSISAINSMKEMVKELHANGIEVML
Sbjct: 421 VNAVLLEPIFPFDEKEGPYFPSHFFSPTNIYGPSGGSISAINSMKEMVKELHANGIEVML 480
Query: 481 EVVFTNTAEDGTLQGIDDSSYYFTNRVANLEGKNALNCNYPVVQQLLLDSLRYWVTEFHI 540
EVVFTNTAEDGTLQGIDDSSYYFTNRVANLEGKNALNCNYPVVQQLLLDSLRYWVTEFHI
Sbjct: 481 EVVFTNTAEDGTLQGIDDSSYYFTNRVANLEGKNALNCNYPVVQQLLLDSLRYWVTEFHI 540
Query: 541 DGFCFINASSLLRGYRGELLSRPPLVEAIAFDPLLSKTKIIADFWDPHESISKETRFPHW 600
DGFCFINASSLLRGYRGELLSRPPLVEAIAFDPLLSKTKIIADFWDPHESISKETRFPHW
Sbjct: 541 DGFCFINASSLLRGYRGELLSRPPLVEAIAFDPLLSKTKIIADFWDPHESISKETRFPHW 600
Query: 601 KRWAEVNYKFCSDVRDFFRGEGLVSSLATRLCGSGDIFSDGRGPAFSFNFIARNFGLPLV 660
KRWAEVNYKFCSDVRDFFRGEGLVSSLATRLCGSGDIFSDGRGPAFSFNFIARNFGLPLV
Sbjct: 601 KRWAEVNYKFCSDVRDFFRGEGLVSSLATRLCGSGDIFSDGRGPAFSFNFIARNFGLPLV 660
Query: 661 DLVSFSSSKLASELSWNCGEEGPTSNIEVLEKRLKLIRNFIFVLFVSLGVPVLNMGDECG 720
DLVSFSSSKLASELSWNCGEEGPTSNIEVLEKRLKLIRNFIFVLFVSLGVPVLNMGDECG
Sbjct: 661 DLVSFSSSKLASELSWNCGEEGPTSNIEVLEKRLKLIRNFIFVLFVSLGVPVLNMGDECG 720
Query: 721 QSSGGSIAFKDRRPFDWDLLKTGFGTQTTQFITFFSSFRARRFDLFQSRNFLKGENIDWY 780
QSSGGSIAFKDRRPFDWDLLKTGFGTQTTQFITFFSSFRARRFDLFQSRNFLKGENIDWY
Sbjct: 721 QSSGGSIAFKDRRPFDWDLLKTGFGTQTTQFITFFSSFRARRFDLFQSRNFLKGENIDWY 780
Query: 781 GNNLSPPQWEDPSCKFLAVMLRADKEENGLSAENPEPQSDIFMAFNASDQSINVALPEPS 840
GNNLSPPQWEDPSCKFLAVMLRADKEENGLSAENPEPQSDIFMAFNASDQSINVALPEPS
Sbjct: 781 GNNLSPPQWEDPSCKFLAVMLRADKEENGLSAENPEPQSDIFMAFNASDQSINVALPEPS 840
Query: 841 EGTSWFRLIDTALSFPGFFSSDGELVPTVESVTYEMQAHSCALFEAKSYSD 892
EGTSWFRLIDTALSFPGFFSSDGELVPTVESVTYEMQAHSCALFEAKSYSD
Sbjct: 841 EGTSWFRLIDTALSFPGFFSSDGELVPTVESVTYEMQAHSCALFEAKSYSD 891
BLAST of Moc09g07920 vs. ExPASy TrEMBL
Match:
A0A0A0K551 (Aamy domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G392380 PE=4 SV=1)
HSP 1 Score: 1469.1 bits (3802), Expect = 0.0e+00
Identity = 713/885 (80.56%), Postives = 784/885 (88.59%), Query Frame = 0
Query: 7 MATFPSSLVVQPYNVHNFGVTEAFRLAASDHHTHGHMIKHQSGKRDEVRMLAHGESRAGF 66
MATF SSLV+QP+ V+N GVTE+ +LAASDH T+G K+Q GK DE RMLAHGE++ G
Sbjct: 1 MATFLSSLVIQPFCVYNCGVTESPKLAASDHLTYGQKTKYQFGKMDEARMLAHGENKVGA 60
Query: 67 AKSSRYNLSKAYASFGISVEKNGWRFGISGKSKEQEKVATYLFRTECGDLVNVLVRKTGS 126
KSS NLSK YA GISV K+G R GI GKSKEQ +VATYLFRTE GDLVNV V K GS
Sbjct: 61 VKSSHRNLSKTYAKSGISVGKSGQRLGIGGKSKEQRRVATYLFRTEFGDLVNVFVGKKGS 120
Query: 127 GFMVSIELSSVQLANIEETPMLSWGVYRSDSALVTPNIQSSLPDDTIGTIETPFVNTSEG 186
F V+IE+ S+QL +I+E +LSWGVYRSDSALVTPN +SS PD+T G ETPFV TSEG
Sbjct: 121 TFTVNIEVPSMQLVSIDEALLLSWGVYRSDSALVTPNFESSPPDETTGATETPFVKTSEG 180
Query: 187 KFAVELEFDAKHVPFYLSFLLKHPVGVDSGSSEIRSHRKTSFCVPVGFGRGYPSPLGVSI 246
KF+VELEFDAKH PFYLSF+LK+P+GVDSGSSEIRSH+KTSF VPVGFGRGYPSPLG+SI
Sbjct: 181 KFSVELEFDAKHTPFYLSFVLKYPMGVDSGSSEIRSHKKTSFSVPVGFGRGYPSPLGLSI 240
Query: 247 SGDGSMNFSIFSSSAESVVLCLYNDSTSEKPVIELDLDRYINRSGNIWHVSFESVSNFVS 306
SGDGS+NFSIFSSSAES+VLCLYNDSTSEKP++ELDLD YINRSGNIWH SFE S FVS
Sbjct: 241 SGDGSVNFSIFSSSAESLVLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGASKFVS 300
Query: 307 YGYLCKGSKSHKKQDGVEISRILVDPYAKVLTTSSHKTHGHGLGLPSKLLGQICKVPAFD 366
YGY CKGSKSH+ QDG+E+SRI+VDPYAK+L S K+ G GLGLPSK LGQI KVP FD
Sbjct: 301 YGYQCKGSKSHENQDGLEVSRIVVDPYAKILAPSIPKSSGQGLGLPSKFLGQISKVPTFD 360
Query: 367 WDGEVRPNLAMEKLFTYRLNVKRFTVDKSSQLTTDIAGTFAGLTKKLLHFKNLGVNAVLL 426
WDGEV PNL MEKLF YRLNV+RFT+DKSSQL DIAGTF+GLTKKLLHFKNLGVNAVLL
Sbjct: 361 WDGEVHPNLPMEKLFVYRLNVERFTMDKSSQLPADIAGTFSGLTKKLLHFKNLGVNAVLL 420
Query: 427 EPIFPFDEKEGPYFPSHFFSPTNIYGPSGGSISAINSMKEMVKELHANGIEVMLEVVFTN 486
EPIF FDEKEGPYFP HFFSPTN YGPSG SISAINSMKEMVKELHANG+EV+LEVV+T+
Sbjct: 421 EPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVILEVVYTH 480
Query: 487 TAEDGTLQGIDDSSYYFTNRVANLEGKNALNCNYPVVQQLLLDSLRYWVTEFHIDGFCFI 546
T+ +G LQGIDDSSYYFTNRVANLE K+ALNCNYP+VQQLLLDSLRYWVTEFH+DGFCF+
Sbjct: 481 TSGNGALQGIDDSSYYFTNRVANLEEKSALNCNYPIVQQLLLDSLRYWVTEFHVDGFCFV 540
Query: 547 NASSLLRGYRGELLSRPPLVEAIAFDPLLSKTKIIADFWDPHESISKETRFPHWKRWAEV 606
NAS LLRG+ GELLSRPP VEAIAFDPLLSKTK++ADFWDP E SKETRFPHWKRWAEV
Sbjct: 541 NASFLLRGHHGELLSRPPFVEAIAFDPLLSKTKLVADFWDPQELESKETRFPHWKRWAEV 600
Query: 607 NYKFCSDVRDFFRGEGLVSSLATRLCGSGDIFSDGRGPAFSFNFIARNFGLPLVDLVSFS 666
N KFCSD+RDFFRGEGL+SSLATRLCGSGD+FSDGRGPAFSFNFIARN GLPLVDLVSFS
Sbjct: 601 NSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS 660
Query: 667 SSKLASELSWNCGEEGPTSNIEVLEKRLKLIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 726
+S LASELSWNCGEEGPTSN++VLEKRLK IRNFIFVLFVSLGVPVLNMGDECGQSSGGS
Sbjct: 661 NSNLASELSWNCGEEGPTSNLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 720
Query: 727 IAFKDRRPFDWDLLKTGFGTQTTQFITFFSSFRARRFDLFQSRNFLKGENIDWYGNNLSP 786
+AF D+R F+WDLLKT FGTQTTQFI F SSFR+RRFDLFQ+RNFLKGENIDW+ NN SP
Sbjct: 721 VAFNDKRSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLFQNRNFLKGENIDWFDNNQSP 780
Query: 787 PQWEDPSCKFLAVMLRADKEENGLSAENPEPQSDIFMAFNASDQSINVALPEPSEGTSWF 846
PQWED SCKFLAVMLRADKEEN ENP+ +S+IFM FNASDQS +VALPEP EGTSWF
Sbjct: 781 PQWEDASCKFLAVMLRADKEENESITENPKTRSNIFMVFNASDQSESVALPEPLEGTSWF 840
Query: 847 RLIDTALSFPGFFSSDGELVPTVESVTYEMQAHSCALFEAKSYSD 892
R++DTAL FPGFFSSDGELVP SVTYE+QAHSCALFEAKS +D
Sbjct: 841 RVVDTALPFPGFFSSDGELVPMTGSVTYEIQAHSCALFEAKSAND 885
BLAST of Moc09g07920 vs. ExPASy TrEMBL
Match:
A0A5A7UR47 (Isoamylase 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold328G00230 PE=4 SV=1)
HSP 1 Score: 1468.4 bits (3800), Expect = 0.0e+00
Identity = 714/882 (80.95%), Postives = 778/882 (88.21%), Query Frame = 0
Query: 7 MATFPSSLVVQPYNVHNFGVTEAFRLAASDHHTHGHMIKHQSGKRDEVRMLAHGESRAGF 66
MATF SSLV+QP+ VHN GVTE+ +LA SDH T+G K+QSGK DE RMLAHGES+ G
Sbjct: 1 MATFLSSLVIQPFCVHNCGVTESLKLAVSDHLTYGQKTKYQSGKMDEARMLAHGESKVGA 60
Query: 67 AKSSRYNLSKAYASFGISVEKNGWRFGISGKSKEQEKVATYLFRTECGDLVNVLVRKTGS 126
KSS NLSKAY + GISV K+G R GI GKSKEQ KVATYLFRTE GDLVNV V KTGS
Sbjct: 61 VKSSHRNLSKAYVTSGISVGKSGQRLGIGGKSKEQRKVATYLFRTEFGDLVNVFVGKTGS 120
Query: 127 GFMVSIELSSVQLANIEETPMLSWGVYRSDSALVTPNIQSSLPDDTIGTIETPFVNTSEG 186
F V+IE+SS+QL + +ET +LSW VYRSDSA VT N QSS PD T G ETPFV TSEG
Sbjct: 121 AFTVNIEISSMQLVSTDETLLLSWRVYRSDSAPVTSNFQSSPPDGTTGATETPFVKTSEG 180
Query: 187 KFAVELEFDAKHVPFYLSFLLKHPVGVDSGSSEIRSHRKTSFCVPVGFGRGYPSPLGVSI 246
KF+VELEFDAKH PFYLSF+LK P+GVDSGSSEIRSHRKTSF VPVGFGRGYPSP G+SI
Sbjct: 181 KFSVELEFDAKHTPFYLSFVLKFPMGVDSGSSEIRSHRKTSFSVPVGFGRGYPSPSGLSI 240
Query: 247 SGDGSMNFSIFSSSAESVVLCLYNDSTSEKPVIELDLDRYINRSGNIWHVSFESVSNFVS 306
SGDGS+NFSIFSS+AESVVLCLYNDSTSEKP++ELDLD YINRSGNIWH SFE VSNFVS
Sbjct: 241 SGDGSINFSIFSSTAESVVLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGVSNFVS 300
Query: 307 YGYLCKGSKSHKKQDGVEISRILVDPYAKVLTTSSHKTHGHGLGLPSKLLGQICKVPAFD 366
YGY CKG KSH+ QDG+E+SRI+VDPYAK+L S K+ GLG P K LGQI VPAFD
Sbjct: 301 YGYQCKGLKSHENQDGLEVSRIVVDPYAKILAPSIPKSRDQGLGFPLKFLGQISIVPAFD 360
Query: 367 WDGEVRPNLAMEKLFTYRLNVKRFTVDKSSQLTTDIAGTFAGLTKKLLHFKNLGVNAVLL 426
WDGEVRPNL MEKLF YRLNVKRFT+DKSSQL DIAGTF+GLTKKLLH KNLGVNAVLL
Sbjct: 361 WDGEVRPNLPMEKLFVYRLNVKRFTIDKSSQLPADIAGTFSGLTKKLLHLKNLGVNAVLL 420
Query: 427 EPIFPFDEKEGPYFPSHFFSPTNIYGPSGGSISAINSMKEMVKELHANGIEVMLEVVFTN 486
EPIF FDEKEGPYFP HFFSPTN YGPSG SISAINSMKEMVKELHANG+EV++EVV+T+
Sbjct: 421 EPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVIVEVVYTH 480
Query: 487 TAEDGTLQGIDDSSYYFTNRVANLEGKNALNCNYPVVQQLLLDSLRYWVTEFHIDGFCFI 546
T+ DG LQGIDDSSYYF NRVANLE ++ALNCNYP+VQQLLLDSLRYWVTEFH+DGFCF+
Sbjct: 481 TSVDGALQGIDDSSYYFANRVANLEERSALNCNYPIVQQLLLDSLRYWVTEFHVDGFCFV 540
Query: 547 NASSLLRGYRGELLSRPPLVEAIAFDPLLSKTKIIADFWDPHESISKETRFPHWKRWAEV 606
NAS LL+G+ GELLSRPP VEAIAFDP+LSKTK++ADFWDP E +SKETRFPHWKRWAEV
Sbjct: 541 NASFLLQGHHGELLSRPPFVEAIAFDPILSKTKLVADFWDPQELVSKETRFPHWKRWAEV 600
Query: 607 NYKFCSDVRDFFRGEGLVSSLATRLCGSGDIFSDGRGPAFSFNFIARNFGLPLVDLVSFS 666
N KFCSD+RDFFRGEGL+SSLATRLCGSGD+FSDGRGPAFSFNFIARN GLPLVDLVSFS
Sbjct: 601 NSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS 660
Query: 667 SSKLASELSWNCGEEGPTSNIEVLEKRLKLIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 726
+S LASELSWNCGEEGPT+ ++VLEKRLK IRNFIFVLFVSLGVPVLNMGDECGQSSGGS
Sbjct: 661 NSNLASELSWNCGEEGPTNTLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 720
Query: 727 IAFKDRRPFDWDLLKTGFGTQTTQFITFFSSFRARRFDLFQSRNFLKGENIDWYGNNLSP 786
+AF DRR F+WDLLKT FGTQTTQFI F SSFR+RRFDLFQSRNFLKGENIDW+ NN SP
Sbjct: 721 VAFNDRRSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLFQSRNFLKGENIDWFDNNQSP 780
Query: 787 PQWEDPSCKFLAVMLRADKEENGLSAENPEPQSDIFMAFNASDQSINVALPEPSEGTSWF 846
PQWED SCKFLAVMLRADKEEN ENP+ +SDIFMAFNASDQS +VALPEPSEGTSWF
Sbjct: 781 PQWEDASCKFLAVMLRADKEENESITENPKTRSDIFMAFNASDQSESVALPEPSEGTSWF 840
Query: 847 RLIDTALSFPGFFSSDGELVPTVESVTYEMQAHSCALFEAKS 889
R++DTAL FPGFFSSDGELVP SVTYEMQAHSCALFEAKS
Sbjct: 841 RVVDTALPFPGFFSSDGELVPMTGSVTYEMQAHSCALFEAKS 882
BLAST of Moc09g07920 vs. ExPASy TrEMBL
Match:
A0A6J1GPU3 (isoamylase 2, chloroplastic isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111456039 PE=4 SV=1)
HSP 1 Score: 1467.6 bits (3798), Expect = 0.0e+00
Identity = 714/885 (80.68%), Postives = 785/885 (88.70%), Query Frame = 0
Query: 7 MATFPSSLVVQPYNVHNFGVTEAFRLAASDHHTHGHMIKHQSGKRDEVRMLAHGESRAGF 66
MATF SSL +QP+ +N G TE+F+LA SD+HT+G + +QSGK DE RM AHGES+ GF
Sbjct: 1 MATFLSSL-IQPFCGYNRGGTESFKLAVSDYHTYGQKMNYQSGKLDEARMQAHGESKVGF 60
Query: 67 AKSSRYNLSKAYASFGISVEKNGWRFGISGKSKEQEKVATYLFRTECGDLVNVLVRKTGS 126
KS N+ +AYA ISV K+G +FG GK++E KVATYLFRTE GDLV V K GS
Sbjct: 61 TKSLHRNVCRAYAPSRISVGKSGQKFGTGGKTEEDRKVATYLFRTEFGDLVYAFVGKRGS 120
Query: 127 GFMVSIELSSVQLANIEETPMLSWGVYRSDSALVTPNIQSSLPDDTIGTIETPFVNTSEG 186
GFM SIE+SS++LA+I+ET +LSWGVYRS+SA+VTPN QS LP++TIG ETPFV TSEG
Sbjct: 121 GFMFSIEISSLKLASIDETLLLSWGVYRSNSAIVTPNFQSILPEETIGATETPFVRTSEG 180
Query: 187 KFAVELEFDAKHVPFYLSFLLKHPVGVDSGSSEIRSHRKTSFCVPVGFGRGYPSPLGVSI 246
KFAVELEFDAKH PFYLSFLLK+PVGVDSGSSEI+SHRKTSF VPVGFGRGYPSPLG+SI
Sbjct: 181 KFAVELEFDAKHDPFYLSFLLKYPVGVDSGSSEIKSHRKTSFSVPVGFGRGYPSPLGLSI 240
Query: 247 SGDGSMNFSIFSSSAESVVLCLYNDSTSEKPVIELDLDRYINRSGNIWHVSFESVSNFVS 306
SGDGS+NFSIFSS+AESVVLCLYNDSTSEKPV+ELDLD YINRSGNIWH SFE SNFVS
Sbjct: 241 SGDGSINFSIFSSTAESVVLCLYNDSTSEKPVLELDLDPYINRSGNIWHASFEGASNFVS 300
Query: 307 YGYLCKGSKSHKKQDGVEISRILVDPYAKVLTTSSHKTHGHGLGLPSKLLGQICKVPAFD 366
YGY C+GSK H+ QD E+SRILVDPYAK+LT S KT G GLGLPSK LGQIC VPAF
Sbjct: 301 YGYRCRGSKFHENQDEAEVSRILVDPYAKILTASIPKTRGQGLGLPSKFLGQICNVPAFS 360
Query: 367 WDGEVRPNLAMEKLFTYRLNVKRFTVDKSSQLTTDIAGTFAGLTKKLLHFKNLGVNAVLL 426
WDGEVRPNL MEKLF YRLNVK FTVDKSSQL D AGTF+GLT+KLLHFK LGVNAVLL
Sbjct: 361 WDGEVRPNLPMEKLFIYRLNVKHFTVDKSSQLPIDTAGTFSGLTQKLLHFKKLGVNAVLL 420
Query: 427 EPIFPFDEKEGPYFPSHFFSPTNIYGPSGGSISAINSMKEMVKELHANGIEVMLEVVFTN 486
EPIFPFDEK+GPYFP HFFSPTNIY PSG SIS++NS+KEMVKELHANG+EV+LEVVFT+
Sbjct: 421 EPIFPFDEKDGPYFPFHFFSPTNIYVPSGASISSLNSVKEMVKELHANGVEVILEVVFTH 480
Query: 487 TAEDGTLQGIDDSSYYFTNRVANLEGKNALNCNYPVVQQLLLDSLRYWVTEFHIDGFCFI 546
TA DG LQGIDDS YYF NRVAN EGK+ALNCN+P+VQQLLLDSLR+WVTEFHIDGFCFI
Sbjct: 481 TAMDGALQGIDDSCYYFVNRVANSEGKSALNCNHPIVQQLLLDSLRHWVTEFHIDGFCFI 540
Query: 547 NASSLLRGYRGELLSRPPLVEAIAFDPLLSKTKIIADFWDPHESISKETRFPHWKRWAEV 606
NASSLL+G+ GELLSRPP VEAIAFDPLLSKTKIIADFWDP ES+ KETRFPHWKRWAE+
Sbjct: 541 NASSLLQGHHGELLSRPPFVEAIAFDPLLSKTKIIADFWDPQESVPKETRFPHWKRWAEL 600
Query: 607 NYKFCSDVRDFFRGEGLVSSLATRLCGSGDIFSDGRGPAFSFNFIARNFGLPLVDLVSFS 666
N KF +D+++FFRGEGLVSSLATRLCGSGD+FSDGRGPAFSFNFIARN GLPLVDLVSFS
Sbjct: 601 NSKFSNDIKEFFRGEGLVSSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS 660
Query: 667 SSKLASELSWNCGEEGPTSNIEVLEKRLKLIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 726
SSKLASELSWNCGEEGPTSNIEVLEKRLK IRNFIFVLFVSLGVPVLNMGDECGQSSGGS
Sbjct: 661 SSKLASELSWNCGEEGPTSNIEVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 720
Query: 727 IAFKDRRPFDWDLLKTGFGTQTTQFITFFSSFRARRFDLFQSRNFLKGENIDWYGNNLSP 786
+AFKDR+ F+WD LKT FGTQTTQFITF SSFR+RRFDLFQSRNFLK ENIDW+ NN SP
Sbjct: 721 VAFKDRKSFNWDSLKTDFGTQTTQFITFLSSFRSRRFDLFQSRNFLKVENIDWFDNNQSP 780
Query: 787 PQWEDPSCKFLAVMLRADKEENGLSAENPEPQSDIFMAFNASDQSINVALPEPSEGTSWF 846
P WEDPSCKFLAVMLRADKEE+G ENP+PQSDIFMAFNASDQS++VALPEPSEGT+WF
Sbjct: 781 PDWEDPSCKFLAVMLRADKEESGSITENPKPQSDIFMAFNASDQSVSVALPEPSEGTNWF 840
Query: 847 RLIDTALSFPGFFSSDGELVPTVESVTYEMQAHSCALFEAKSYSD 892
RL+DTALSFPGFFSSDGE VPT VTY MQAHSC LFEAKS++D
Sbjct: 841 RLVDTALSFPGFFSSDGEPVPTTSPVTYGMQAHSCTLFEAKSFND 884
BLAST of Moc09g07920 vs. ExPASy TrEMBL
Match:
A0A1S3BRF8 (isoamylase 2, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103492696 PE=4 SV=1)
HSP 1 Score: 1466.8 bits (3796), Expect = 0.0e+00
Identity = 713/882 (80.84%), Postives = 777/882 (88.10%), Query Frame = 0
Query: 7 MATFPSSLVVQPYNVHNFGVTEAFRLAASDHHTHGHMIKHQSGKRDEVRMLAHGESRAGF 66
MATF SSLV+QP+ VHN GVTE+ +LA SDH T+G K+QSGK DE RMLAHGES+ G
Sbjct: 1 MATFLSSLVIQPFCVHNCGVTESLKLAVSDHLTYGQKTKYQSGKMDEARMLAHGESKVGA 60
Query: 67 AKSSRYNLSKAYASFGISVEKNGWRFGISGKSKEQEKVATYLFRTECGDLVNVLVRKTGS 126
KSS NLSKAY + GI V K+G R GI GKSKEQ KVATYLFRTE GDLVNV V KTGS
Sbjct: 61 VKSSHRNLSKAYVTSGILVGKSGQRLGIGGKSKEQRKVATYLFRTEFGDLVNVFVGKTGS 120
Query: 127 GFMVSIELSSVQLANIEETPMLSWGVYRSDSALVTPNIQSSLPDDTIGTIETPFVNTSEG 186
F V+IE+SS+QL + +ET +LSW VYRSDSA VT N QSS PD T G ETPFV TSEG
Sbjct: 121 AFTVNIEISSMQLVSTDETLLLSWRVYRSDSAPVTSNFQSSPPDGTTGATETPFVKTSEG 180
Query: 187 KFAVELEFDAKHVPFYLSFLLKHPVGVDSGSSEIRSHRKTSFCVPVGFGRGYPSPLGVSI 246
KF+VELEFDAKH PFYLSF+LK P+GVDSGSSEIRSHRKTSF VPVGFGRGYPSPLG+SI
Sbjct: 181 KFSVELEFDAKHTPFYLSFVLKFPMGVDSGSSEIRSHRKTSFSVPVGFGRGYPSPLGLSI 240
Query: 247 SGDGSMNFSIFSSSAESVVLCLYNDSTSEKPVIELDLDRYINRSGNIWHVSFESVSNFVS 306
SGDGS+NFSIFSS+AESVVLCLYNDSTSEKP++ELDLD YINRSGNIWH SFE SNFVS
Sbjct: 241 SGDGSINFSIFSSTAESVVLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGASNFVS 300
Query: 307 YGYLCKGSKSHKKQDGVEISRILVDPYAKVLTTSSHKTHGHGLGLPSKLLGQICKVPAFD 366
YGY CKG KSH+ QDG+E+SRI+VDPYAK+L S K+ GLG P K LGQI VPAFD
Sbjct: 301 YGYQCKGLKSHENQDGLEVSRIVVDPYAKILAPSIPKSRDQGLGFPLKFLGQISIVPAFD 360
Query: 367 WDGEVRPNLAMEKLFTYRLNVKRFTVDKSSQLTTDIAGTFAGLTKKLLHFKNLGVNAVLL 426
WDGEVRPNL MEKLF YRLNVKRFT+DKSSQL DIAGTF+GLTKKLLH KNLGVNAVLL
Sbjct: 361 WDGEVRPNLPMEKLFVYRLNVKRFTIDKSSQLPADIAGTFSGLTKKLLHLKNLGVNAVLL 420
Query: 427 EPIFPFDEKEGPYFPSHFFSPTNIYGPSGGSISAINSMKEMVKELHANGIEVMLEVVFTN 486
EPIF FDEKEGPYFP HFFSPTN YGPSG SISAINSMKEMVKELHANG+EV++EVV+T+
Sbjct: 421 EPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVIVEVVYTH 480
Query: 487 TAEDGTLQGIDDSSYYFTNRVANLEGKNALNCNYPVVQQLLLDSLRYWVTEFHIDGFCFI 546
T+ DG LQGIDDSSYYF NRVANLE ++ALNCNYP+VQQLLLDSLRYWVTEFH+DGFCF+
Sbjct: 481 TSVDGALQGIDDSSYYFANRVANLEERSALNCNYPIVQQLLLDSLRYWVTEFHVDGFCFV 540
Query: 547 NASSLLRGYRGELLSRPPLVEAIAFDPLLSKTKIIADFWDPHESISKETRFPHWKRWAEV 606
NAS LL+G+ GELLSRPP VEAIAFDP+LSKTK++ADFWDP E +SKETRFPHWKRWAEV
Sbjct: 541 NASFLLQGHHGELLSRPPFVEAIAFDPILSKTKLVADFWDPQELVSKETRFPHWKRWAEV 600
Query: 607 NYKFCSDVRDFFRGEGLVSSLATRLCGSGDIFSDGRGPAFSFNFIARNFGLPLVDLVSFS 666
N KFCSD+RDFFRGEGL+SSLATRLCGSGD+FSDGRGPAFSFNFIARN GLPLVDLVSFS
Sbjct: 601 NSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS 660
Query: 667 SSKLASELSWNCGEEGPTSNIEVLEKRLKLIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 726
+S LASELSWNCGEEGPT+ ++VLEKRLK IRNFIFVLFVSLGVPVLNMGDECGQSSGGS
Sbjct: 661 NSNLASELSWNCGEEGPTNTLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 720
Query: 727 IAFKDRRPFDWDLLKTGFGTQTTQFITFFSSFRARRFDLFQSRNFLKGENIDWYGNNLSP 786
+AF DRR F+WDLLKT FGTQTTQFI F SSFR+RRFDLFQSRNFLKGENIDW+ NN SP
Sbjct: 721 VAFNDRRSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLFQSRNFLKGENIDWFDNNQSP 780
Query: 787 PQWEDPSCKFLAVMLRADKEENGLSAENPEPQSDIFMAFNASDQSINVALPEPSEGTSWF 846
PQWED SCKFLAVMLRADKEEN ENP+ +SDIFMAFNASDQS +VALPEPSEGTSWF
Sbjct: 781 PQWEDASCKFLAVMLRADKEENESITENPKTRSDIFMAFNASDQSESVALPEPSEGTSWF 840
Query: 847 RLIDTALSFPGFFSSDGELVPTVESVTYEMQAHSCALFEAKS 889
R++DTAL FPGFFSSDGELVP SVTYEMQAHSCALFEAKS
Sbjct: 841 RVVDTALPFPGFFSSDGELVPMTGSVTYEMQAHSCALFEAKS 882
BLAST of Moc09g07920 vs. TAIR 10
Match:
AT1G03310.2 (debranching enzyme 1 )
HSP 1 Score: 924.5 bits (2388), Expect = 6.6e-269
Identity = 459/824 (55.70%), Postives = 595/824 (72.21%), Query Frame = 0
Query: 72 YNLSKAYASFGISVEKNGWRFGISGKSKEQEKVATYLFRTECGDLVNVLVRKTGSGFMVS 131
Y SK AS S+E ++ + +KV +Y FRT+ G LV V V K + +
Sbjct: 64 YQFSKICAS-KTSIELREALSSRRAEADDLKKVTSYSFRTKAGALVKVKVEKKREKYSIL 123
Query: 132 IELSSVQLANIEETPM-LSWGVYRSDSALVTP-NIQSSLPDDTIGTIETPFVNTSEGKFA 191
+ +SS++L+ +++ + + WGVYRSDS+ P + ++S D T ET FV +S +
Sbjct: 124 VYVSSLELSGDDKSRLVMVWGVYRSDSSCFLPLDFENSSQDSQTHTTETTFVKSSLSELM 183
Query: 192 VELEFDAKHVPFYLSFLLKHPVGVDSGSSEIRSHRKTSFCVPVGFGRGYPSPLGVSIS-G 251
+ LEFD K PFYLSF LK G D E+ +HR T FC+PVGF G+P PLG+S
Sbjct: 184 LGLEFDGKESPFYLSFHLKLVSGRDPDGQEMLTHRDTDFCIPVGFTAGHPLPLGLSSGPD 243
Query: 252 DGSMNFSIFSSSAESVVLCLYNDSTSEKPVIELDLDRYINRSGNIWHVSFESVSNFVSYG 311
D S NFS FS S+ +VVLCLY+DST++KP +ELDLD Y+NR+G++WH S ++ +FV YG
Sbjct: 244 DDSWNFSFFSRSSTNVVLCLYDDSTTDKPALELDLDPYVNRTGDVWHASVDNTWDFVRYG 303
Query: 312 YLCKGSKSHKKQDGVEISRILVDPYAKVLTTSSHKTHGHGLGLPSKLLGQICKVPAFDWD 371
Y CK + K+ VE I++DPYA V+ G + K LG + K P+FDW
Sbjct: 304 YRCKETAHSKEDVDVEGEPIVLDPYATVV----------GKSVSQKYLGSLSKSPSFDWG 363
Query: 372 GEVRPNLAMEKLFTYRLNVKRFTVDKSSQLTTDIAGTFAGLTKKLLHFKNLGVNAVLLEP 431
+V PN+ +EKL YRLNVK FT +SS+L +++AGTF+G+ +K+ H K LG NAVLLEP
Sbjct: 364 EDVSPNIPLEKLLVYRLNVKGFTQHRSSKLPSNVAGTFSGVAEKVSHLKTLGTNAVLLEP 423
Query: 432 IFPFDEKEGPYFPSHFFSPTNIYGPSGGSISAINSMKEMVKELHANGIEVMLEVVFTNTA 491
IF F E++GPYFP HFFSP +IYGPS SA+NSMK MVK+LH+ GIEV+LEVVFT+TA
Sbjct: 424 IFSFSEQKGPYFPFHFFSPMDIYGPSNSLESAVNSMKVMVKKLHSEGIEVLLEVVFTHTA 483
Query: 492 EDGTLQGIDDSSYYFTNRVANLEGKNALNCNYPVVQQLLLDSLRYWVTEFHIDGFCFINA 551
+ G L+GIDDSSYY+ R +L+ K+ LNCNYPVVQQL+L+SLRYWVTEFH+DGFCFINA
Sbjct: 484 DSGALRGIDDSSYYYKGRANDLDSKSYLNCNYPVVQQLVLESLRYWVTEFHVDGFCFINA 543
Query: 552 SSLLRGYRGELLSRPPLVEAIAFDPLLSKTKIIADFWDPHESISKETRFPHWKRWAEVNY 611
SSLLRG GE LSRPPLVEAIAFDPLL++TK+IAD WDP E + KE RFPHWKRWAE+N
Sbjct: 544 SSLLRGVHGEQLSRPPLVEAIAFDPLLAETKLIADCWDPLEMMPKEVRFPHWKRWAELNT 603
Query: 612 KFCSDVRDFFRGEGLVSSLATRLCGSGDIFSDGRGPAFSFNFIARNFGLPLVDLVSFSSS 671
++C +VR+F RG G++S LATR+CGSGD+F+DGRGPAFSFN+I+RN GL LVD+VSFS
Sbjct: 604 RYCRNVRNFLRGRGVLSDLATRICGSGDVFTDGRGPAFSFNYISRNSGLSLVDIVSFSGP 663
Query: 672 KLASELSWNCGEEGPTSNIEVLEKRLKLIRNFIFVLFVSLGVPVLNMGDECGQSSGGSIA 731
+LASELSWNCGEEG T+ VL++RLK IRNF+F+ ++SLGVPVLNMGDECG S+ GS
Sbjct: 664 ELASELSWNCGEEGATNKSAVLQRRLKQIRNFLFIQYISLGVPVLNMGDECGISTRGSPL 723
Query: 732 FKDRRPFDWDLLKTGFGTQTTQFITFFSSFRARRFDLFQSRNFLKGENIDWYGNNLSPPQ 791
+ R+PFDW+LL + FGTQ TQFI+F +S RARR D+FQ R+FLK ENI WY N+ + P+
Sbjct: 724 LESRKPFDWNLLASAFGTQITQFISFMTSVRARRSDVFQRRDFLKPENIVWYANDQTTPK 783
Query: 792 WEDPSCKFLAVMLRADKEENGLS--AENPEPQS-DIFMAFNASDQSINVALPEPSEGTSW 851
WEDP+ KFLA+ ++++ EE + AE EP+S D+F+ FNASD +V LP +G+ W
Sbjct: 784 WEDPASKFLALEIKSESEEEETASLAEPNEPKSNDLFIGFNASDHPESVVLPSLPDGSKW 843
Query: 852 FRLIDTALSFPGFFSSDGELV----PTVESVTYEMQAHSCALFE 886
RL+DTAL FPGFFS +GE V P + V YEM+ +SC LFE
Sbjct: 844 RRLVDTALPFPGFFSVEGETVVAEEPLQQLVVYEMKPYSCTLFE 876
BLAST of Moc09g07920 vs. TAIR 10
Match:
AT1G03310.1 (debranching enzyme 1 )
HSP 1 Score: 924.5 bits (2388), Expect = 6.6e-269
Identity = 459/824 (55.70%), Postives = 595/824 (72.21%), Query Frame = 0
Query: 72 YNLSKAYASFGISVEKNGWRFGISGKSKEQEKVATYLFRTECGDLVNVLVRKTGSGFMVS 131
Y SK AS S+E ++ + +KV +Y FRT+ G LV V V K + +
Sbjct: 64 YQFSKICAS-KTSIELREALSSRRAEADDLKKVTSYSFRTKAGALVKVKVEKKREKYSIL 123
Query: 132 IELSSVQLANIEETPM-LSWGVYRSDSALVTP-NIQSSLPDDTIGTIETPFVNTSEGKFA 191
+ +SS++L+ +++ + + WGVYRSDS+ P + ++S D T ET FV +S +
Sbjct: 124 VYVSSLELSGDDKSRLVMVWGVYRSDSSCFLPLDFENSSQDSQTHTTETTFVKSSLSELM 183
Query: 192 VELEFDAKHVPFYLSFLLKHPVGVDSGSSEIRSHRKTSFCVPVGFGRGYPSPLGVSIS-G 251
+ LEFD K PFYLSF LK G D E+ +HR T FC+PVGF G+P PLG+S
Sbjct: 184 LGLEFDGKESPFYLSFHLKLVSGRDPDGQEMLTHRDTDFCIPVGFTAGHPLPLGLSSGPD 243
Query: 252 DGSMNFSIFSSSAESVVLCLYNDSTSEKPVIELDLDRYINRSGNIWHVSFESVSNFVSYG 311
D S NFS FS S+ +VVLCLY+DST++KP +ELDLD Y+NR+G++WH S ++ +FV YG
Sbjct: 244 DDSWNFSFFSRSSTNVVLCLYDDSTTDKPALELDLDPYVNRTGDVWHASVDNTWDFVRYG 303
Query: 312 YLCKGSKSHKKQDGVEISRILVDPYAKVLTTSSHKTHGHGLGLPSKLLGQICKVPAFDWD 371
Y CK + K+ VE I++DPYA V+ G + K LG + K P+FDW
Sbjct: 304 YRCKETAHSKEDVDVEGEPIVLDPYATVV----------GKSVSQKYLGSLSKSPSFDWG 363
Query: 372 GEVRPNLAMEKLFTYRLNVKRFTVDKSSQLTTDIAGTFAGLTKKLLHFKNLGVNAVLLEP 431
+V PN+ +EKL YRLNVK FT +SS+L +++AGTF+G+ +K+ H K LG NAVLLEP
Sbjct: 364 EDVSPNIPLEKLLVYRLNVKGFTQHRSSKLPSNVAGTFSGVAEKVSHLKTLGTNAVLLEP 423
Query: 432 IFPFDEKEGPYFPSHFFSPTNIYGPSGGSISAINSMKEMVKELHANGIEVMLEVVFTNTA 491
IF F E++GPYFP HFFSP +IYGPS SA+NSMK MVK+LH+ GIEV+LEVVFT+TA
Sbjct: 424 IFSFSEQKGPYFPFHFFSPMDIYGPSNSLESAVNSMKVMVKKLHSEGIEVLLEVVFTHTA 483
Query: 492 EDGTLQGIDDSSYYFTNRVANLEGKNALNCNYPVVQQLLLDSLRYWVTEFHIDGFCFINA 551
+ G L+GIDDSSYY+ R +L+ K+ LNCNYPVVQQL+L+SLRYWVTEFH+DGFCFINA
Sbjct: 484 DSGALRGIDDSSYYYKGRANDLDSKSYLNCNYPVVQQLVLESLRYWVTEFHVDGFCFINA 543
Query: 552 SSLLRGYRGELLSRPPLVEAIAFDPLLSKTKIIADFWDPHESISKETRFPHWKRWAEVNY 611
SSLLRG GE LSRPPLVEAIAFDPLL++TK+IAD WDP E + KE RFPHWKRWAE+N
Sbjct: 544 SSLLRGVHGEQLSRPPLVEAIAFDPLLAETKLIADCWDPLEMMPKEVRFPHWKRWAELNT 603
Query: 612 KFCSDVRDFFRGEGLVSSLATRLCGSGDIFSDGRGPAFSFNFIARNFGLPLVDLVSFSSS 671
++C +VR+F RG G++S LATR+CGSGD+F+DGRGPAFSFN+I+RN GL LVD+VSFS
Sbjct: 604 RYCRNVRNFLRGRGVLSDLATRICGSGDVFTDGRGPAFSFNYISRNSGLSLVDIVSFSGP 663
Query: 672 KLASELSWNCGEEGPTSNIEVLEKRLKLIRNFIFVLFVSLGVPVLNMGDECGQSSGGSIA 731
+LASELSWNCGEEG T+ VL++RLK IRNF+F+ ++SLGVPVLNMGDECG S+ GS
Sbjct: 664 ELASELSWNCGEEGATNKSAVLQRRLKQIRNFLFIQYISLGVPVLNMGDECGISTRGSPL 723
Query: 732 FKDRRPFDWDLLKTGFGTQTTQFITFFSSFRARRFDLFQSRNFLKGENIDWYGNNLSPPQ 791
+ R+PFDW+LL + FGTQ TQFI+F +S RARR D+FQ R+FLK ENI WY N+ + P+
Sbjct: 724 LESRKPFDWNLLASAFGTQITQFISFMTSVRARRSDVFQRRDFLKPENIVWYANDQTTPK 783
Query: 792 WEDPSCKFLAVMLRADKEENGLS--AENPEPQS-DIFMAFNASDQSINVALPEPSEGTSW 851
WEDP+ KFLA+ ++++ EE + AE EP+S D+F+ FNASD +V LP +G+ W
Sbjct: 784 WEDPASKFLALEIKSESEEEETASLAEPNEPKSNDLFIGFNASDHPESVVLPSLPDGSKW 843
Query: 852 FRLIDTALSFPGFFSSDGELV----PTVESVTYEMQAHSCALFE 886
RL+DTAL FPGFFS +GE V P + V YEM+ +SC LFE
Sbjct: 844 RRLVDTALPFPGFFSVEGETVVAEEPLQQLVVYEMKPYSCTLFE 876
BLAST of Moc09g07920 vs. TAIR 10
Match:
AT4G09020.1 (isoamylase 3 )
HSP 1 Score: 375.2 bits (962), Expect = 1.5e-103
Identity = 254/704 (36.08%), Postives = 363/704 (51.56%), Query Frame = 0
Query: 237 GYPSPLGVSISGDGSMNFSIFSSSAESVVLCLY-----NDSTSEKPVIELDLDRYINRSG 296
G SPLGVS D +NF++FS +A SV LCL D T + +IEL LD +N++G
Sbjct: 88 GEVSPLGVS-QVDKGINFALFSQNATSVTLCLSLSQSGKDDTDDDGMIELVLDPSVNKTG 147
Query: 297 NIWHVSFESVS-NFVSYGYLCKGSKSHKKQDGVEISRILVDPYAKVLTTSSHKTHGHGLG 356
+ WH+ E + N V YGY G ++ + S +L+DPYAK++ H + G
Sbjct: 148 DTWHICVEDLPLNNVLYGYRVDGPGEWQQGHRFDRSILLLDPYAKLV--KGHSSFGDSSQ 207
Query: 357 LPSKLLGQI-CKVPAFDWDGEVR-PNLAMEKLFTYRLNVKRFTVDKSSQLTTDIAGTFAG 416
++ G + FDW + + PN+ + L Y +NV+ FT D+SS + I G++ G
Sbjct: 208 KFAQFYGTYDFESSPFDWGDDYKFPNIPEKDLVIYEMNVRAFTADESSGMDPAIGGSYLG 267
Query: 417 LTKKLLHFKNLGVNAVLLEPIFPFDEKE--------------GPYFPSHFFSPTNIYGP- 476
+K+ H ++LG+NAV L P+F FDE E Y +FF+P + Y
Sbjct: 268 FIEKIPHLQDLGINAVELLPVFEFDELELQRRSNPRDHMVNTWGYSTVNFFAPMSRYASG 327
Query: 477 SGGSISAINSMKEMVKELHANGIEVMLEVVF--TNTAED-----GTLQGIDDSSYYF--- 536
G I A KEMVK LH+ GIEV+L+VV+ TN A+D + +GID+ YY
Sbjct: 328 EGDPIKASKEFKEMVKALHSAGIEVILDVVYNHTNEADDKYPYTTSFRGIDNKVYYMLDP 387
Query: 537 TNRVANLEG-KNALNCNYPVVQQLLLDSLRYWVTEFHIDGFCFINASSLLRGYRGELLSR 596
N++ N G N LNCN+PVV +L+LDSLR+WVTE+H+DGF F AS L R G LS
Sbjct: 388 NNQLLNFSGCGNTLNCNHPVVMELILDSLRHWVTEYHVDGFRFDLASVLCRATDGSPLSA 447
Query: 597 PPLVEAIAFDPLLSKTKIIADFWDPHESISKETRFPHWKRWAEVNYKFCSDVRDFFRGE- 656
PPL+ AIA D +LS+ KIIA+ WD + +FP+W RWAE N + DVR F +G+
Sbjct: 448 PPLIRAIAKDSVLSRCKIIAEPWD-CGGLYLVGKFPNWDRWAEWNGMYRDDVRRFIKGDS 507
Query: 657 GLVSSLATRLCGSGDIFS-DGRGPAFSFNFIARNFGLPLVDLVSFS-----------SSK 716
G+ S ATR+ GS D++ + R P NF+ + G L DLVS++ +
Sbjct: 508 GMKGSFATRVSGSSDLYQVNQRKPYHGVNFVIAHDGFTLRDLVSYNFKHNEANGEGGNDG 567
Query: 717 LASELSWNCGEEGPTSNIEVLEKRLKLIRNFIFVLFVSLGVPVLNMGDECGQSS-GGSIA 776
SWNCG EG T + + R + ++NF L +S G P++ MGDE G + G + +
Sbjct: 568 CNDNHSWNCGFEGETGDAHIKSLRTRQMKNFHLALMISQGTPMMLMGDEYGHTRYGNNNS 627
Query: 777 F---KDRRPFDWDLLKTGFGTQTTQFITFFSSFRARRFD--LFQSRNFLKGENIDWYGNN 836
+ F W L + FFS R + + NFL I W+ +N
Sbjct: 628 YGHDTSLNNFQWKEL----DAKKQNHFRFFSEVIKFRHSHHVLKHENFLTQGEITWHEDN 687
Query: 837 LSPPQWEDPSCKFLAVMLRADKEENGLSAENPEPQSDIFMAFNASDQSINVALPEPSEGT 888
W++ KFLA L +G+ DI++AFNA D + +P+P G
Sbjct: 688 -----WDNSESKFLAFTL-----HDGIGGR------DIYVAFNAHDYFVKALIPQPPPGK 747
BLAST of Moc09g07920 vs. TAIR 10
Match:
AT2G39930.1 (isoamylase 1 )
HSP 1 Score: 323.2 bits (827), Expect = 6.7e-88
Identity = 229/690 (33.19%), Postives = 335/690 (48.55%), Query Frame = 0
Query: 237 GYPSPLGVSISGDGSMNFSIFSSSAESVVLCL--YNDSTSEKPVIELDLDRYINRSGNIW 296
G PSP G ++ DG +NFS++S+++ S +CL +D K E+ LD NR+G++W
Sbjct: 72 GLPSPFGPTVRDDG-VNFSVYSTNSVSATICLISLSDLRQNKVTEEIQLDPSRNRTGHVW 131
Query: 297 HVSFESVSNFVSYGYLCKGSKSHKKQDGVEISRILVDPYAKVLTTSSHKTHGHGLGLPSK 356
HV + YGY G S ++ + S IL+DPYAK + + + LG
Sbjct: 132 HVFLRGDFKDMLYGYRFDGKFSPEEGHYYDSSNILLDPYAKAIIS---RDEFGVLGPDDN 191
Query: 357 LLGQI-CKVPA----FDWDGEVRPNLAMEKLFTYRLNVKRFTVDKSSQLTTDIAGTFAGL 416
Q+ C VP FDW+G++ L + L Y ++V+ FT +SS++ + GT+ G+
Sbjct: 192 CWPQMACMVPTREEEFDWEGDMHLKLPQKDLVIYEMHVRGFTRHESSKI--EFPGTYQGV 251
Query: 417 TKKLLHFKNLGVNAVLLEPIFPFDEKE---------------GPYFPSHFFSPTNIYGPS 476
+KL H K LG+N + L P F+E E Y FFSP Y +
Sbjct: 252 AEKLDHLKELGINCIELMPCHEFNELEYYSYNTILGDHRVNFWGYSTIGFFSPMIRYASA 311
Query: 477 GGSI---SAINSMKEMVKELHANGIEVMLEVVFTNTAEDG------TLQGIDDSSYYF-- 536
+ AIN K +VKE H GIEV+++VV +TAE + +G+D+S YY
Sbjct: 312 SSNNFAGRAINEFKILVKEAHKRGIEVIMDVVLNHTAEGNEKGPIFSFRGVDNSVYYMLA 371
Query: 537 -TNRVANLEG-KNALNCNYPVVQQLLLDSLRYWVTEFHIDGFCFINASSLLRG------- 596
N G N NCN+PVV+Q +LD LRYWVTE H+DGF F S + R
Sbjct: 372 PKGEFYNYSGCGNTFNCNHPVVRQFILDCLRYWVTEMHVDGFRFDLGSIMSRSSSLWDAA 431
Query: 597 ------YRGELL------SRPPLVEAIAFDPLLSKTKIIADFWDPHESISKETRFPHWKR 656
G+LL S PP+++ I+ DP+L K+IA+ WD + + FPHW
Sbjct: 432 NVYGADVEGDLLTTGTPISCPPVIDMISNDPILRGVKLIAEAWDA-GGLYQVGMFPHWGI 491
Query: 657 WAEVNYKFCSDVRDFFRG-EGLVSSLATRLCGSGDIFSDGRGPAFSFNFIARNFGLPLVD 716
W+E N KF VR F +G +G + A LCGS +++ GR P S NFI + G L D
Sbjct: 492 WSEWNGKFRDVVRQFIKGTDGFSGAFAECLCGSPNLYQGGRKPWHSINFICAHDGFTLAD 551
Query: 717 LVSFSS-SKLAS----------ELSWNCGEEGPTSNIEVLEKRLKLIRNFIFVLFVSLGV 776
LV++++ + LA+ SWNCGEEG ++I V R + +RNF L VS GV
Sbjct: 552 LVTYNNKNNLANGEENNDGENHNYSWNCGEEGDFASISVKRLRKRQMRNFFVSLMVSQGV 611
Query: 777 PVLNMGDECGQSSGGS----IAFKDRRPFDWDLLKTGFGTQTTQFITFFSSFRARRFDLF 836
P++ MGDE G + GG+ F WD K + +F FR +
Sbjct: 612 PMIYMGDEYGHTKGGNNNTYCHDNYMNYFRWD-KKEEAHSDFFRFCRILIKFR-DECESL 671
Query: 837 QSRNFLKGENIDWYGNNLSPPQWEDPSCKFLAVMLRADKEENGLSAENPEPQSDIFMAFN 857
+F + + W+G P W + S +F+A L + +I++AFN
Sbjct: 672 GLNDFPTAKRLQWHGLAPEIPNWSETS-RFVAFSL------------VDSVKKEIYVAFN 731
BLAST of Moc09g07920 vs. TAIR 10
Match:
AT3G20440.2 (Alpha amylase family protein )
HSP 1 Score: 55.5 bits (132), Expect = 2.6e-07
Identity = 43/161 (26.71%), Postives = 74/161 (45.96%), Query Frame = 0
Query: 405 TFAGLTKKLL-HFKNLGVNAVLLEPIFPF-DEKEGPYFPSHFFSPTNIYGPSGGSISAIN 464
TF TKK+L H K G NA+ L + D Y ++FF+ ++ YG +
Sbjct: 416 TFEEFTKKVLPHVKRAGYNAIQLIGVPEHKDYFTVGYRVTNFFAASSRYGTP-------D 475
Query: 465 SMKEMVKELHANGIEVMLEVVFTNTAED-----GTLQGIDDSSYYFTNRVANLE-GKNAL 524
K +V E H G+ V L++V + A D G +D +++ R + G
Sbjct: 476 DFKRLVDEAHGLGLLVFLDIVHSYAAADQMVGLSLFDGSNDCYFHYGKRGHHKHWGTRMF 535
Query: 525 NCNYPVVQQLLLDSLRYWVTEFHIDGFCFINASSLLRGYRG 558
V L+ +L +W+TE+ +DG+ F + +S++ + G
Sbjct: 536 KYGDLDVLHFLISNLNWWITEYQVDGYQFHSLASMIYTHNG 569
The following BLAST results are available for this feature:
| Match Name | E-value | Identity | Description | |
|---|
| XP_022150231.1 | 0.0e+00 | 100.00 | isoamylase 2, chloroplastic [Momordica charantia] | [more] |
| XP_038898155.1 | 0.0e+00 | 83.62 | isoamylase 2, chloroplastic [Benincasa hispida] | [more] |
| KAG7014422.1 | 0.0e+00 | 80.67 | Isoamylase 2, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma] | [more] |
| XP_004136188.1 | 0.0e+00 | 80.56 | isoamylase 2, chloroplastic [Cucumis sativus] >KGN44865.1 hypothetical protein C... | [more] |
| KAA0057598.1 | 0.0e+00 | 80.95 | isoamylase 2 [Cucumis melo var. makuwa] >TYK20996.1 isoamylase 2 [Cucumis melo v... | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| Q8L735 | 9.3e-268 | 55.70 | Isoamylase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ISA2 PE=1 SV=2 | [more] |
| Q6AU80 | 3.8e-221 | 53.42 | Isoamylase 2, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=ISA2 PE=... | [more] |
| Q9M0S5 | 2.1e-102 | 36.08 | Isoamylase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ISA3 PE=1 SV=2 | [more] |
| B9G434 | 1.5e-100 | 35.57 | Isoamylase 3, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=ISA3 PE=... | [more] |
| D0TZF0 | 3.4e-92 | 33.77 | Isoamylase 1, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=ISA1 PE=... | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| A0A6J1DAX4 | 0.0e+00 | 100.00 | isoamylase 2, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111018448 PE=4 ... | [more] |
| A0A0A0K551 | 0.0e+00 | 80.56 | Aamy domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G392380 PE=4 S... | [more] |
| A0A5A7UR47 | 0.0e+00 | 80.95 | Isoamylase 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold328G00230 P... | [more] |
| A0A6J1GPU3 | 0.0e+00 | 80.68 | isoamylase 2, chloroplastic isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC11145... | [more] |
| A0A1S3BRF8 | 0.0e+00 | 80.84 | isoamylase 2, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103492696 PE=4 SV=1 | [more] |
