Moc08g35970 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc08g35970
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	leucine-rich repeat extensin-like protein 2
Location	chr8: 26533685 .. 26534101 (+)
RNA-Seq Expression	Moc08g35970
Synteny	Moc08g35970

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGCCTCCTCTTCTCTCCTCTTCTCTTCTCCTCCTCTTCATTCTCCTCACCGACGCCGATACCTCACCGTTGGTATCGGTCGGTTTGGAGAATCCCGATGTCGAAATCAAATGTGGATCCTGCCCTTGTTCCAAGCCATGCATTCAGCGGTCGCCGCCGCCGTATTGCAACCCAGCGCTGTCACCCCCGCCACCGCCCCCGAGGTTCATTTATACGACAGGTGTTCCCGGTCACTTGTCACCGCCGCCACCGAGGTTCATTTATACGACCGGCGTCCCCGATAACGTGTACCAAACCGATGTGAACAGCCAATGGTATTATTTCTCCGGTGGAACGTCGCCGAAGCCAGCGGTCACGGCGGCACTGGTGGTGGCGCTTTGGCTGTGGAGCTTTGGATCTCATGGCGTTTGGTAA

mRNA sequence

ATGGCGCCTCCTCTTCTCTCCTCTTCTCTTCTCCTCCTCTTCATTCTCCTCACCGACGCCGATACCTCACCGTTGGTATCGGTCGGTTTGGAGAATCCCGATGTCGAAATCAAATGTGGATCCTGCCCTTGTTCCAAGCCATGCATTCAGCGGTCGCCGCCGCCGTATTGCAACCCAGCGCTGTCACCCCCGCCACCGCCCCCGAGGTTCATTTATACGACAGGTGTTCCCGGTCACTTGTCACCGCCGCCACCGAGGTTCATTTATACGACCGGCGTCCCCGATAACGTGTACCAAACCGATGTGAACAGCCAATGGTATTATTTCTCCGGTGGAACGTCGCCGAAGCCAGCGGTCACGGCGGCACTGGTGGTGGCGCTTTGGCTGTGGAGCTTTGGATCTCATGGCGTTTGGTAA

Coding sequence (CDS)

ATGGCGCCTCCTCTTCTCTCCTCTTCTCTTCTCCTCCTCTTCATTCTCCTCACCGACGCCGATACCTCACCGTTGGTATCGGTCGGTTTGGAGAATCCCGATGTCGAAATCAAATGTGGATCCTGCCCTTGTTCCAAGCCATGCATTCAGCGGTCGCCGCCGCCGTATTGCAACCCAGCGCTGTCACCCCCGCCACCGCCCCCGAGGTTCATTTATACGACAGGTGTTCCCGGTCACTTGTCACCGCCGCCACCGAGGTTCATTTATACGACCGGCGTCCCCGATAACGTGTACCAAACCGATGTGAACAGCCAATGGTATTATTTCTCCGGTGGAACGTCGCCGAAGCCAGCGGTCACGGCGGCACTGGTGGTGGCGCTTTGGCTGTGGAGCTTTGGATCTCATGGCGTTTGGTAA

Protein sequence

MAPPLLSSSLLLLFILLTDADTSPLVSVGLENPDVEIKCGSCPCSKPCIQRSPPPYCNPALSPPPPPPRFIYTTGVPGHLSPPPPRFIYTTGVPDNVYQTDVNSQWYYFSGGTSPKPAVTAALVVALWLWSFGSHGVW

Homology

[Close]

BLAST of Moc08g35970 vs. NCBI nr
Match: KAG6581215.1 (hypothetical protein SDJN03_21217, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 141.7 bits (356), Expect = 4.6e-30
Identity = 80/135 (59.26%), Postives = 95/135 (70.37%), Query Frame = 0


Query: 1   MAP-PLLSSSLLLLFILLTDADTSPLVSVGLENPDVEIKCGSCPCSKPCIQR-------S 60
           MAP   +  SL  LF+ L  AD+ P+  V L++P+ EIKCGSCPCS PC+++        
Sbjct: 1   MAPSAAIPLSLSFLFLFLHLADSQPVSPVDLQDPNSEIKCGSCPCSNPCVKQFPPPPPPP 60

Query: 61  PPPYCNPALSPPPPPPRFIYTTGVPGHLSPPPPRFIYTTGVPDNVYQTDVNSQWYYFSGG 120
           PPP C PAL PPPPPPRFIYT+  P    PPPPRFIYTTGVP N+YQ D N++WYYFSG 
Sbjct: 61  PPPNCKPALPPPPPPPRFIYTSLSP----PPPPRFIYTTGVPGNLYQIDANNRWYYFSGT 120

Query: 121 TSPKPAVTAALVVAL 128
           T  +PA TAALVVAL
Sbjct: 121 TRNQPA-TAALVVAL 130

[Close]

BLAST of Moc08g35970 vs. NCBI nr
Match: KAG7017949.1 (hypothetical protein SDJN02_19815, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 140.2 bits (352), Expect = 1.3e-29
Identity = 79/132 (59.85%), Postives = 94/132 (71.21%), Query Frame = 0


Query: 1   MAP-PLLSSSLLLLFILLTDADTSPLVSVGLENPDVEIKCGSCPCSKPCIQR----SPPP 60
           MAP   +  SL  LF+ L  AD+ P+  V  ++P+ EIKCGSCPCS PC+++     PPP
Sbjct: 1   MAPSAAIPLSLSFLFLFLHLADSQPVSPVDPQDPNSEIKCGSCPCSNPCVKQFPPPPPPP 60

Query: 61  YCNPALSPPPPPPRFIYTTGVPGHLSPPPPRFIYTTGVPDNVYQTDVNSQWYYFSGGTSP 120
            C PAL PPPPPPRFIYT+  P    PPPPRFIYTTGVP N+YQ D N++WYYFSG T  
Sbjct: 61  NCKPALPPPPPPPRFIYTSLSP----PPPPRFIYTTGVPGNLYQIDANNRWYYFSGTTRN 120

Query: 121 KPAVTAALVVAL 128
           +PA TAALVVAL
Sbjct: 121 QPA-TAALVVAL 127

[Close]

BLAST of Moc08g35970 vs. NCBI nr
Match: XP_023528316.1 (leucine-rich repeat extensin-like protein 3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 137.1 bits (344), Expect = 1.1e-28
Identity = 78/135 (57.78%), Postives = 91/135 (67.41%), Query Frame = 0


Query: 1   MAP-PLLSSSLLLLFILLTDADTSPLVSVGLENPDVEIKCGSCPCSKPCIQR-------S 60
           MAP   +  SL  LF+ L  AD+ P   V  ++P+ EIKCGSCPCS PC+++        
Sbjct: 3   MAPSAAIPLSLSFLFLFLHLADSQPFSPVDPQDPNSEIKCGSCPCSNPCVKQFPPPPPPP 62

Query: 61  PPPYCNPALSPPPPPPRFIYTTGVPGHLSPPPPRFIYTTGVPDNVYQTDVNSQWYYFSGG 120
           PPP C PAL PPPPPPRFIYT   P    PPPPRFIYTTGVP N+YQ D N++WYYFSG 
Sbjct: 63  PPPNCKPALPPPPPPPRFIYTALSP----PPPPRFIYTTGVPGNLYQIDANNRWYYFSGT 122

Query: 121 TSPKPAVTAALVVAL 128
           T  +PA TA LVVAL
Sbjct: 123 TRNRPA-TAVLVVAL 132

[Close]

BLAST of Moc08g35970 vs. NCBI nr
Match: KAA0060761.1 (formin-like protein 3 [Cucumis melo var. makuwa] >TYK01496.1 formin-like protein 3 [Cucumis melo var. makuwa])

HSP 1 Score: 134.0 bits (336), Expect = 9.6e-28
Identity = 75/131 (57.25%), Postives = 89/131 (67.94%), Query Frame = 0


Query: 5   LLSSSLLLLFILLTDADTSPLVSVGLENPDVEIKCGSCPCSKPCIQR-SPPP-------Y 64
           LLS S L L +LL  + TS  +S+  +NPD EIKCGSCPCS PCIQ+ SPPP        
Sbjct: 8   LLSFSFLSLLLLLAHSQTSSPISIDPQNPDAEIKCGSCPCSNPCIQQLSPPPPPPPPPSP 67

Query: 65  CNPALSPPPPPPRFIYTTGVPGHLSPPPPRFIYTTGVPDNVYQTDVNSQWYYFSGGTSPK 124
           C P +S  PPPPRFIYT   P   +PPPPRF YTTGVP N+Y+ D N+ WYYFSG T  +
Sbjct: 68  CTPTVSSRPPPPRFIYTMSSP---APPPPRFTYTTGVPGNLYEIDANNSWYYFSGTTGTR 127

Query: 125 PAVTAALVVAL 128
           P + AA+VVAL
Sbjct: 128 PGM-AAVVVAL 134

[Close]

BLAST of Moc08g35970 vs. NCBI nr
Match: XP_022984145.1 (probable pathogenesis-related protein ARB_02861 [Cucurbita maxima])

HSP 1 Score: 133.7 bits (335), Expect = 1.3e-27
Identity = 76/134 (56.72%), Postives = 93/134 (69.40%), Query Frame = 0


Query: 1   MAP-PLLSSSLLLLFILLTDADTSPLVSVGLENPDVEIKCGSCPCSKPCIQR------SP 60
           MAP   +  SL  LF+ L  A++ P+  V  ++P+ EIKCGSCPCS PC+++       P
Sbjct: 1   MAPSAAIPLSLSFLFLFLHLANSQPVSPVDPQDPNSEIKCGSCPCSNPCVKQFPPPPPPP 60

Query: 61  PPYCNPALSPPPPPPRFIYTTGVPGHLSPPPPRFIYTTGVPDNVYQTDVNSQWYYFSGGT 120
           PP C PAL PPPPPPRFIYT+  P    PPPPRFIYTTGVP N+YQ D N++WYYFS GT
Sbjct: 61  PPNCKPALPPPPPPPRFIYTSLSP----PPPPRFIYTTGVPGNLYQIDANNRWYYFS-GT 120

Query: 121 SPKPAVTAALVVAL 128
           +   + TAALVVAL
Sbjct: 121 TRNWSATAALVVAL 129

[Close]

BLAST of Moc08g35970 vs. ExPASy TrEMBL
Match: A0A5A7V4T2 (Formin-like protein 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold451G00500 PE=4 SV=1)

HSP 1 Score: 134.0 bits (336), Expect = 4.7e-28
Identity = 75/131 (57.25%), Postives = 89/131 (67.94%), Query Frame = 0


Query: 5   LLSSSLLLLFILLTDADTSPLVSVGLENPDVEIKCGSCPCSKPCIQR-SPPP-------Y 64
           LLS S L L +LL  + TS  +S+  +NPD EIKCGSCPCS PCIQ+ SPPP        
Sbjct: 8   LLSFSFLSLLLLLAHSQTSSPISIDPQNPDAEIKCGSCPCSNPCIQQLSPPPPPPPPPSP 67

Query: 65  CNPALSPPPPPPRFIYTTGVPGHLSPPPPRFIYTTGVPDNVYQTDVNSQWYYFSGGTSPK 124
           C P +S  PPPPRFIYT   P   +PPPPRF YTTGVP N+Y+ D N+ WYYFSG T  +
Sbjct: 68  CTPTVSSRPPPPRFIYTMSSP---APPPPRFTYTTGVPGNLYEIDANNSWYYFSGTTGTR 127

Query: 125 PAVTAALVVAL 128
           P + AA+VVAL
Sbjct: 128 PGM-AAVVVAL 134

[Close]

BLAST of Moc08g35970 vs. ExPASy TrEMBL
Match: A0A6J1J7W0 (probable pathogenesis-related protein ARB_02861 OS=Cucurbita maxima OX=3661 GN=LOC111482528 PE=4 SV=1)

HSP 1 Score: 133.7 bits (335), Expect = 6.1e-28
Identity = 76/134 (56.72%), Postives = 93/134 (69.40%), Query Frame = 0


Query: 1   MAP-PLLSSSLLLLFILLTDADTSPLVSVGLENPDVEIKCGSCPCSKPCIQR------SP 60
           MAP   +  SL  LF+ L  A++ P+  V  ++P+ EIKCGSCPCS PC+++       P
Sbjct: 1   MAPSAAIPLSLSFLFLFLHLANSQPVSPVDPQDPNSEIKCGSCPCSNPCVKQFPPPPPPP 60

Query: 61  PPYCNPALSPPPPPPRFIYTTGVPGHLSPPPPRFIYTTGVPDNVYQTDVNSQWYYFSGGT 120
           PP C PAL PPPPPPRFIYT+  P    PPPPRFIYTTGVP N+YQ D N++WYYFS GT
Sbjct: 61  PPNCKPALPPPPPPPRFIYTSLSP----PPPPRFIYTTGVPGNLYQIDANNRWYYFS-GT 120

Query: 121 SPKPAVTAALVVAL 128
           +   + TAALVVAL
Sbjct: 121 TRNWSATAALVVAL 129

[Close]

BLAST of Moc08g35970 vs. ExPASy TrEMBL
Match: A0A0A0L7R0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G346900 PE=4 SV=1)

HSP 1 Score: 125.9 bits (315), Expect = 1.3e-25
Identity = 70/131 (53.44%), Postives = 83/131 (63.36%), Query Frame = 0


Query: 5   LLSSSLLLLFILLTDADTSPLVSVGLENPDVEIKCGSCPCSKPCIQR-SPPP-------- 64
           LLS S L   +LL ++ TS  + V  +NPD EIKCGSCPCS PCIQ+ SPPP        
Sbjct: 10  LLSFSFLSHLLLLANSQTSSPILVDPQNPDAEIKCGSCPCSNPCIQQLSPPPPPPPPPPP 69

Query: 65  --YCNPALSPPPPPPRFIYTTGVPGHLSPPPPRFIYTTGVPDNVYQTDVNSQWYYFSGGT 124
              C P +S  PPPPRFIYTT  P   +PPPPRF YTTGVP N+Y+ D N+ WYYFSG  
Sbjct: 70  PSPCTPTVSSRPPPPRFIYTTSSP---APPPPRFTYTTGVPGNLYEIDANNSWYYFSGTR 129

[Close]

BLAST of Moc08g35970 vs. ExPASy TrEMBL
Match: A0A6J1GAW3 (leucine-rich repeat extensin-like protein 2 OS=Cucurbita moschata OX=3662 GN=LOC111452509 PE=4 SV=1)

HSP 1 Score: 98.2 bits (243), Expect = 2.8e-17
Identity = 64/130 (49.23%), Postives = 79/130 (60.77%), Query Frame = 0


Query: 1   MAPPLLSSSLLLLFILLTDADTSPLVSVGLENPDVEIKCGSCP-CSKPCIQRSPPPYCNP 60
           MAP    S+ + L +LL+   TSP +S    +PD EI C S   CS P +Q+  P   +P
Sbjct: 1   MAP----SAAIFLSLLLSFIQTSPPISASPLDPDTEINCDSTTLCSNPSVQQLQP---SP 60

Query: 61  ALSPPP-PPPRFIYTTGVPGHLSPPPP-RFIYTTGVPDNVYQTDVNSQWYYFSGGTSPKP 120
            L PPP PPPRF YTT      SPPPP RFIYTTGVP ++YQ D  ++WYYFSG     P
Sbjct: 61  PLPPPPLPPPRFTYTT----LSSPPPPLRFIYTTGVPSDLYQNDEKNRWYYFSGAVGKPP 119

Query: 121 AVTAALVVAL 128
           A+ A +VV L
Sbjct: 121 AMAALVVVGL 119

[Close]

BLAST of Moc08g35970 vs. ExPASy TrEMBL
Match: A0A5J5T6H2 (Uncharacterized protein OS=Gossypium barbadense OX=3634 GN=ES319_A12G057600v1 PE=4 SV=1)

HSP 1 Score: 87.0 bits (214), Expect = 6.5e-14
Identity = 46/102 (45.10%), Postives = 55/102 (53.92%), Query Frame = 0


Query: 33  PDVEIKCGSCPCSKPCIQRSPPPYCNPALSPPPPPPRFIYTTGVPGHLSPPPPRFIYTTG 92
           P  EIKCG+CPC  PC Q+S          PPPPPPRF Y+  +P    PPPPRF Y TG
Sbjct: 47  PTTEIKCGACPCVNPCGQQS---------LPPPPPPRFTYSNTLPS--PPPPPRFYYVTG 106

Query: 93  VPDNVYQTDVNSQWYYFSGGTSPKPAVTAALVVALWLWSFGS 135
           VP  +Y  D + +W YFS G           V  +WLW  G+
Sbjct: 107 VPGQLYVVDPDDRWRYFSNGGQ---NFVGGFVAFIWLWVVGA 134

[Close]

BLAST of Moc08g35970 vs. TAIR 10
Match: AT3G03350.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 45.4 bits (106), Expect = 4.2e-05
Identity = 22/46 (47.83%), Postives = 30/46 (65.22%), Query Frame = 0


Query: 65  PPPPRFIYTTGVPGHLSPPPPRFIYTTGVPDNVYQTDVNSQWYYFS 111
           PPPPR  Y +      SPPPP+++Y TGVP  +Y+T+ + QW Y S
Sbjct: 30  PPPPRKTYCS--KSSSSPPPPKYMYVTGVPGELYRTEPDDQWGYNS 73

[Close]

BLAST of Moc08g35970 vs. TAIR 10
Match: AT3G03350.2 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 45.4 bits (106), Expect = 4.2e-05
Identity = 22/46 (47.83%), Postives = 30/46 (65.22%), Query Frame = 0


Query: 65  PPPPRFIYTTGVPGHLSPPPPRFIYTTGVPDNVYQTDVNSQWYYFS 111
           PPPPR  Y +      SPPPP+++Y TGVP  +Y+T+ + QW Y S
Sbjct: 30  PPPPRKTYCS--KSSSSPPPPKYMYVTGVPGELYRTEPDDQWGYNS 73

[Close]

BLAST of Moc08g35970 vs. TAIR 10
Match: AT1G23040.1 (hydroxyproline-rich glycoprotein family protein )

HSP 1 Score: 41.2 bits (95), Expect = 7.9e-04
Identity = 27/68 (39.71%), Postives = 33/68 (48.53%), Query Frame = 0


Query: 44  CSKPCIQR----------SPPPYCNPALSPPPPPPRFIYTTGVPGHLSPPPPRFIYTTGV 102
           CS  CIQ            PPP C P  + PPPPP+ + +   P    PPP  F+Y TG 
Sbjct: 51  CSPSCIQNPPPPSPPPPSPPPPACPPPPALPPPPPKKVSSYCPP----PPPANFLYITGP 110

The following BLAST results are available for this feature:

BLAST of Moc08g35970 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6581215.1	4.6e-30	59.26	hypothetical protein SDJN03_21217, partial [Cucurbita argyrosperma subsp. sorori...	[more]
KAG7017949.1	1.3e-29	59.85	hypothetical protein SDJN02_19815, partial [Cucurbita argyrosperma subsp. argyro...	[more]
XP_023528316.1	1.1e-28	57.78	leucine-rich repeat extensin-like protein 3 [Cucurbita pepo subsp. pepo]	[more]
KAA0060761.1	9.6e-28	57.25	formin-like protein 3 [Cucumis melo var. makuwa] >TYK01496.1 formin-like protein...	[more]
XP_022984145.1	1.3e-27	56.72	probable pathogenesis-related protein ARB_02861 [Cucurbita maxima]	[more]

BLAST of Moc08g35970 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of Moc08g35970 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7V4T2	4.7e-28	57.25	Formin-like protein 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold45...	[more]
A0A6J1J7W0	6.1e-28	56.72	probable pathogenesis-related protein ARB_02861 OS=Cucurbita maxima OX=3661 GN=L...	[more]
A0A0A0L7R0	1.3e-25	53.44	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G346900 PE=4 SV=1	[more]
A0A6J1GAW3	2.8e-17	49.23	leucine-rich repeat extensin-like protein 2 OS=Cucurbita moschata OX=3662 GN=LOC...	[more]
A0A5J5T6H2	6.5e-14	45.10	Uncharacterized protein OS=Gossypium barbadense OX=3634 GN=ES319_A12G057600v1 PE...	[more]

BLAST of Moc08g35970 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G03350.1	4.2e-05	47.83	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT3G03350.2	4.2e-05	47.83	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT1G23040.1	7.9e-04	39.71	hydroxyproline-rich glycoprotein family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PANTHER	PTHR35094	LEUCINE-RICH REPEAT EXTENSIN-LIKE PROTEIN 2	coord: 28..126
None	No IPR available	PANTHER	PTHR35094:SF7	PROTEIN, PUTATIVE-RELATED	coord: 28..126
IPR004170	WWE domain	PROSITE	PS50918	WWE	coord: 114..138 score: 9.029105

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc08g35970.1	Moc08g35970.1	mRNA