Moc08g21840 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc08g21840
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
DescriptionRNase H domain-containing protein
Locationchr8: 16076634 .. 16077137 (-)
RNA-Seq ExpressionMoc08g21840
SyntenyMoc08g21840
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCGATTTTTTAGTGCAAGCGAGCTCGGAAGATTTGGCATACTCTTTTTCCACTCTCTACCTAAGTACCCTGCACATTCAAATCCAAGCTTCTTTGATTTTTGGGATGATCTGATCAATAAAATTAACCCAGATAAGAAGGAGTTAGCGGCGATTACCTGTTGGCCAATTTGGTGTGATCGAAATTCAACGGTTCATGGGAAGCAAATTTCTGTACCTGAATCAAAATGTGAGTGGATTCGATCTTATATTAAAAGCTTTTCACAGGCTAAAAATGCCATTTCCCCGCCCGTGCCAGAGACTAGAAGAACTCACCATAGCCAGATTTGGAATCCTCCGTGTGACGGCCATTTGAAGCTCAATACAGATGCTGCATGTAGACCTTCTTCATCAGGTCTGGGATTTCTCTTGCACAAGTCCGATGGCACTTTGGTAGCGGCCAAATCGGTTTTCCTTCCTCAATCCCTTCCACCACTTATTGCTGAAATTAAAGGAATTCTATAA

mRNA sequence

ATGCGATTTTTTAGTGCAAGCGAGCTCGGAAGATTTGGCATACTCTTTTTCCACTCTCTACCTAAGTACCCTGCACATTCAAATCCAAGCTTCTTTGATTTTTGGGATGATCTGATCAATAAAATTAACCCAGATAAGAAGGAGTTAGCGGCGATTACCTGTTGGCCAATTTGGTGTGATCGAAATTCAACGGTTCATGGGAAGCAAATTTCTGTACCTGAATCAAAATGTGAGTGGATTCGATCTTATATTAAAAGCTTTTCACAGGCTAAAAATGCCATTTCCCCGCCCGTGCCAGAGACTAGAAGAACTCACCATAGCCAGATTTGGAATCCTCCGTGTGACGGCCATTTGAAGCTCAATACAGATGCTGCATGTAGACCTTCTTCATCAGGTCTGGGATTTCTCTTGCACAAGTCCGATGGCACTTTGGTAGCGGCCAAATCGGTTTTCCTTCCTCAATCCCTTCCACCACTTATTGCTGAAATTAAAGGAATTCTATAA

Coding sequence (CDS)

ATGCGATTTTTTAGTGCAAGCGAGCTCGGAAGATTTGGCATACTCTTTTTCCACTCTCTACCTAAGTACCCTGCACATTCAAATCCAAGCTTCTTTGATTTTTGGGATGATCTGATCAATAAAATTAACCCAGATAAGAAGGAGTTAGCGGCGATTACCTGTTGGCCAATTTGGTGTGATCGAAATTCAACGGTTCATGGGAAGCAAATTTCTGTACCTGAATCAAAATGTGAGTGGATTCGATCTTATATTAAAAGCTTTTCACAGGCTAAAAATGCCATTTCCCCGCCCGTGCCAGAGACTAGAAGAACTCACCATAGCCAGATTTGGAATCCTCCGTGTGACGGCCATTTGAAGCTCAATACAGATGCTGCATGTAGACCTTCTTCATCAGGTCTGGGATTTCTCTTGCACAAGTCCGATGGCACTTTGGTAGCGGCCAAATCGGTTTTCCTTCCTCAATCCCTTCCACCACTTATTGCTGAAATTAAAGGAATTCTATAA

Protein sequence

MRFFSASELGRFGILFFHSLPKYPAHSNPSFFDFWDDLINKINPDKKELAAITCWPIWCDRNSTVHGKQISVPESKCEWIRSYIKSFSQAKNAISPPVPETRRTHHSQIWNPPCDGHLKLNTDAACRPSSSGLGFLLHKSDGTLVAAKSVFLPQSLPPLIAEIKGIL
Homology
BLAST of Moc08g21840 vs. NCBI nr
Match: XP_022131661.1 (uncharacterized protein LOC111004786 [Momordica charantia])

HSP 1 Score: 149.1 bits (375), Expect = 3.5e-32
Identity = 76/160 (47.50%), Postives = 98/160 (61.25%), Query Frame = 0

Query: 15  LFFHSLPKYPAHSNPSFFDFWDDLINKINPDKKELAAITCWPIWCDRNSTVHGKQISVPE 74
           L F SL   P + N SF D W  L +K+NP +++LAA T W IWCDRNS  HG  +S P 
Sbjct: 20  LMFPSLFTSPINGNLSFMDIWLLLYDKLNPQQRDLAAFTXWAIWCDRNSXAHGSSVSTPA 79

Query: 75  SKCEWIRSYIKSFSQAK--NAISP-----PVPETRRTHHSQIWNPPCDGHLKLNTDAACR 134
            +C WI SY +++SQA+    ISP     P P  R       W PP D  +K+N+DAACR
Sbjct: 80  LRCNWISSYFQNYSQAQENKRISPQQSSVPPPSCR-------WLPPRDSAMKVNSDAACR 139

Query: 135 PSSSGLGFLLHKSDGTLVAAKSVFLPQSLPPLIAEIKGIL 168
            +S+GLG ++    G L+ AKS+FLP  L PL AEI+GIL
Sbjct: 140 STSTGLGLIIRDHFGVLLVAKSMFLPMPLNPLFAEIRGIL 172

BLAST of Moc08g21840 vs. NCBI nr
Match: XP_022158377.1 (uncharacterized protein LOC111024874 [Momordica charantia])

HSP 1 Score: 128.6 bits (322), Expect = 4.9e-26
Identity = 68/165 (41.21%), Postives = 90/165 (54.55%), Query Frame = 0

Query: 3    FFSASELGRFGILFFHSLPKYPAHSNPSFFDFWDDLINKINPDKKELAAITCWPIWCDRN 62
            FF      +     F  L    A  N SF + W  L  ++ P    LAAIT W IW DRN
Sbjct: 1187 FFHCKRARQIWRTLFPFLTCLSAEDNISFLELWSSLTEQLEPKDLNLAAITGWGIWNDRN 1246

Query: 63   STVHGKQISVPESKCEWIRSYIKSFSQAKNAISPPVPETRRTHHSQIWNPPCDGHLKLNT 122
            S +HGKQ+S  E KCEW+  ++ S SQA+ +   P  ++      Q W P     LKLNT
Sbjct: 1247 SLIHGKQVSPVEFKCEWLTPFLDSHSQAQMSNYSPRTQSNHRPVVQYWRPSSSVSLKLNT 1306

Query: 123  DAACRPSSSGLGFLLHKSDGTLVAAKSVFLPQSLPPLIAEIKGIL 168
            DAACR +S+  G ++  S  +LVAA S+ +P  L PL+AEI+ IL
Sbjct: 1307 DAACRGASTSFGCIIRDSSCSLVAATSIRVPFPLSPLLAEIRCIL 1351

BLAST of Moc08g21840 vs. NCBI nr
Match: XP_020249520.1 (uncharacterized protein LOC109826918 [Asparagus officinalis])

HSP 1 Score: 79.0 bits (193), Expect = 4.4e-11
Identity = 44/145 (30.34%), Postives = 72/145 (49.66%), Query Frame = 0

Query: 24  PAHSNPSFFDFWDDLINKINPDKKELAAITCWPIWCDRNSTVHGKQISVPESKCEWIRSY 83
           P  + P+F D+++ ++         L A+TCW IW +RN  V  + +S   SK  WI   
Sbjct: 16  PLPTAPNFKDWFNAMLQN-QQQNLALIAMTCWAIWNNRNEIV-WQALSFTVSKALWIACN 75

Query: 84  IKSFSQAKNAISPPVPETRRTHHSQIWNPPCDGHLKLNTDAA--CRPSSSGLGFLLHKSD 143
           +    + +N + P      +T ++  W+PP  G  KLN DAA        GLGF++    
Sbjct: 76  LLEQWRKENVVPPLATSETKTANNSKWSPPAAGKFKLNVDAAVFTNQRKCGLGFIIRDDQ 135

Query: 144 GTLVAAKSVFLPQSLPPLIAEIKGI 167
           G+ VAA++  +   + P +AE  G+
Sbjct: 136 GSFVAARATQVHGIIDPALAEALGV 158

BLAST of Moc08g21840 vs. NCBI nr
Match: XP_023897447.1 (uncharacterized protein LOC112009345 [Quercus suber])

HSP 1 Score: 78.2 bits (191), Expect = 7.6e-11
Identity = 50/169 (29.59%), Postives = 74/169 (43.79%), Query Frame = 0

Query: 4    FSASELGRFGILFFHSLPKYPAHSNPSFFDFWDDLIN------KINPDKKELAAITCWPI 63
            FS     R  +  + +     A SN     FW  L +        + +K  L     W  
Sbjct: 873  FSVRSAYRVAMGLYQAENAVSASSNSQLQSFWKKLWHLPVPHKSFDEEKIILVVTVAWAF 932

Query: 64   WCDRNSTVHGKQISVPESKCEWIRSYIKSFSQAKNAISPPVPETRRTHHSQIWNPPCDGH 123
            WC+RN   HG +   PE+  +W+  Y+  +S A  ++ P V    R   S  WNPP    
Sbjct: 933  WCNRNEIRHGAEKKSPEAIVQWVNRYLLEYSAATESV-PAV----REEVSVTWNPPPPSI 992

Query: 124  LKLNTDAACRPSSS--GLGFLLHKSDGTLVAAKSVFLPQSLPPLIAEIK 165
            LK+N D A   + +  G+G ++    G +VAA S  +P  L PL  E+K
Sbjct: 993  LKVNVDGATTKNLNFVGVGAVVRDEQGRVVAAMSRKIPAPLGPLEVEVK 1036

BLAST of Moc08g21840 vs. NCBI nr
Match: XP_012852581.1 (PREDICTED: uncharacterized protein LOC105972189 [Erythranthe guttata])

HSP 1 Score: 77.8 bits (190), Expect = 9.9e-11
Identity = 41/124 (33.06%), Postives = 62/124 (50.00%), Query Frame = 0

Query: 24  PAHSNPSFFDFWDDLINKINPDKKELAAITCWPIWCDRNSTVHGKQISVPESKCEWIRSY 83
           P   N SF+++ D L  +++PDK  LA + CW +W  RN  +H +     E    W  +Y
Sbjct: 16  PIAENLSFWNWLDILKRELDPDKLHLAIVVCWKLWERRNKIIHQEDFINLEDMVGWCAAY 75

Query: 84  IKSFSQAKNAISPPVPETRRTHHSQIWNPPCDGHLKLNTDAACRPSSS--GLGFLLHKSD 143
           ++++  A+   +      RR   SQ W PP  G LKLN DAA  P+S    +  +   + 
Sbjct: 76  LRTYDDAQLQHN----NVRRPEFSQTWTPPPIGVLKLNVDAAFPPNSEFHRVSLIARNAT 135

Query: 144 GTLV 146
           GT V
Sbjct: 136 GTCV 135

BLAST of Moc08g21840 vs. ExPASy TrEMBL
Match: A0A6J1BQ49 (uncharacterized protein LOC111004786 OS=Momordica charantia OX=3673 GN=LOC111004786 PE=4 SV=1)

HSP 1 Score: 149.1 bits (375), Expect = 1.7e-32
Identity = 76/160 (47.50%), Postives = 98/160 (61.25%), Query Frame = 0

Query: 15  LFFHSLPKYPAHSNPSFFDFWDDLINKINPDKKELAAITCWPIWCDRNSTVHGKQISVPE 74
           L F SL   P + N SF D W  L +K+NP +++LAA T W IWCDRNS  HG  +S P 
Sbjct: 20  LMFPSLFTSPINGNLSFMDIWLLLYDKLNPQQRDLAAFTXWAIWCDRNSXAHGSSVSTPA 79

Query: 75  SKCEWIRSYIKSFSQAK--NAISP-----PVPETRRTHHSQIWNPPCDGHLKLNTDAACR 134
            +C WI SY +++SQA+    ISP     P P  R       W PP D  +K+N+DAACR
Sbjct: 80  LRCNWISSYFQNYSQAQENKRISPQQSSVPPPSCR-------WLPPRDSAMKVNSDAACR 139

Query: 135 PSSSGLGFLLHKSDGTLVAAKSVFLPQSLPPLIAEIKGIL 168
            +S+GLG ++    G L+ AKS+FLP  L PL AEI+GIL
Sbjct: 140 STSTGLGLIIRDHFGVLLVAKSMFLPMPLNPLFAEIRGIL 172

BLAST of Moc08g21840 vs. ExPASy TrEMBL
Match: A0A6J1DX30 (uncharacterized protein LOC111024874 OS=Momordica charantia OX=3673 GN=LOC111024874 PE=4 SV=1)

HSP 1 Score: 128.6 bits (322), Expect = 2.4e-26
Identity = 68/165 (41.21%), Postives = 90/165 (54.55%), Query Frame = 0

Query: 3    FFSASELGRFGILFFHSLPKYPAHSNPSFFDFWDDLINKINPDKKELAAITCWPIWCDRN 62
            FF      +     F  L    A  N SF + W  L  ++ P    LAAIT W IW DRN
Sbjct: 1187 FFHCKRARQIWRTLFPFLTCLSAEDNISFLELWSSLTEQLEPKDLNLAAITGWGIWNDRN 1246

Query: 63   STVHGKQISVPESKCEWIRSYIKSFSQAKNAISPPVPETRRTHHSQIWNPPCDGHLKLNT 122
            S +HGKQ+S  E KCEW+  ++ S SQA+ +   P  ++      Q W P     LKLNT
Sbjct: 1247 SLIHGKQVSPVEFKCEWLTPFLDSHSQAQMSNYSPRTQSNHRPVVQYWRPSSSVSLKLNT 1306

Query: 123  DAACRPSSSGLGFLLHKSDGTLVAAKSVFLPQSLPPLIAEIKGIL 168
            DAACR +S+  G ++  S  +LVAA S+ +P  L PL+AEI+ IL
Sbjct: 1307 DAACRGASTSFGCIIRDSSCSLVAATSIRVPFPLSPLLAEIRCIL 1351

BLAST of Moc08g21840 vs. ExPASy TrEMBL
Match: A0A5P1EPS3 (RNase H domain-containing protein OS=Asparagus officinalis OX=4686 GN=A4U43_C05F5270 PE=4 SV=1)

HSP 1 Score: 75.9 bits (185), Expect = 1.8e-10
Identity = 44/145 (30.34%), Postives = 70/145 (48.28%), Query Frame = 0

Query: 24  PAHSNPSFFDFWDDLINKINPDKKELAAITCWPIWCDRNSTVHGKQISVPESKCEWIRSY 83
           P  + P+F D+++ ++         L A+TCW IW +RN  V   + S   SK  WI   
Sbjct: 22  PLPTAPNFKDWFNAMLQN-QQQNLALIAMTCWAIWNNRNEIVWQAR-SFTVSKALWIACN 81

Query: 84  IKSFSQAKNAISPPVPETRRTHHSQIWNPPCDGHLKLNTDAA--CRPSSSGLGFLLHKSD 143
           +    + +N + P       T ++  W+PP  G  KLN DAA        GLGF++    
Sbjct: 82  LLEQWRKENVVPPLATGETETANNSKWSPPAAGKFKLNVDAAVFTNQRKCGLGFIIRDDQ 141

Query: 144 GTLVAAKSVFLPQSLPPLIAEIKGI 167
           G+ VAA++  +   + P +AE  G+
Sbjct: 142 GSFVAARATQVHGIIDPALAEALGV 164

BLAST of Moc08g21840 vs. ExPASy TrEMBL
Match: A0A5P1E8M9 (RNase H domain-containing protein OS=Asparagus officinalis OX=4686 GN=A4U43_C10F18610 PE=4 SV=1)

HSP 1 Score: 74.7 bits (182), Expect = 4.1e-10
Identity = 40/120 (33.33%), Postives = 61/120 (50.83%), Query Frame = 0

Query: 49  LAAITCWPIWCDRNSTVHGKQISVPESKCEWIRSYIKSFSQAKNAISPPVPETRRTHHSQ 108
           L A+TCW IW +RN  V  + +S   SK  WI   +    + +N + P      +T ++ 
Sbjct: 11  LIAMTCWAIWNNRNEIV-WQALSFTVSKALWIACNLLEQWRKENVVPPLATSETKTANNS 70

Query: 109 IWNPPCDGHLKLNTDAA--CRPSSSGLGFLLHKSDGTLVAAKSVFLPQSLPPLIAEIKGI 167
            W+PP  G  KLN DAA        GLGF++    G+ VAA++  +   + P +AE  G+
Sbjct: 71  KWSPPAAGKFKLNVDAAVFTNQRKCGLGFIIRDDQGSFVAARATQVHGIIDPALAEALGV 129

BLAST of Moc08g21840 vs. ExPASy TrEMBL
Match: A0A5P1FW40 (RNase H domain-containing protein OS=Asparagus officinalis OX=4686 GN=A4U43_C01F28990 PE=4 SV=1)

HSP 1 Score: 73.9 bits (180), Expect = 6.9e-10
Identity = 42/137 (30.66%), Postives = 64/137 (46.72%), Query Frame = 0

Query: 32  FDFWDDLINKINPDKKELAAITCWPIWCDRNSTVHGKQISVPESKCEWIRSYIKSFSQAK 91
           F  W + + +       L A+TCW IW +RN  V   + S   SK  WI   +    + +
Sbjct: 29  FKGWFNAMLQNQQQNLALIAMTCWAIWNNRNEIVWQAR-SFTVSKALWIACNLLEQWRKE 88

Query: 92  NAISPPVPETRRTHHSQIWNPPCDGHLKLNTDAA--CRPSSSGLGFLLHKSDGTLVAAKS 151
           N + P       T ++  W+PP  G  KLN DAA        GLGF++    G+ VAA++
Sbjct: 89  NVVPPLATSETETANNSKWSPPAAGKFKLNVDAAVFTNQRKCGLGFIIRDDQGSFVAARA 148

Query: 152 VFLPQSLPPLIAEIKGI 167
             +   + P +AE  G+
Sbjct: 149 TQVHGIIDPALAEALGV 164

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022131661.13.5e-3247.50uncharacterized protein LOC111004786 [Momordica charantia][more]
XP_022158377.14.9e-2641.21uncharacterized protein LOC111024874 [Momordica charantia][more]
XP_020249520.14.4e-1130.34uncharacterized protein LOC109826918 [Asparagus officinalis][more]
XP_023897447.17.6e-1129.59uncharacterized protein LOC112009345 [Quercus suber][more]
XP_012852581.19.9e-1133.06PREDICTED: uncharacterized protein LOC105972189 [Erythranthe guttata][more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A6J1BQ491.7e-3247.50uncharacterized protein LOC111004786 OS=Momordica charantia OX=3673 GN=LOC111004... [more]
A0A6J1DX302.4e-2641.21uncharacterized protein LOC111024874 OS=Momordica charantia OX=3673 GN=LOC111024... [more]
A0A5P1EPS31.8e-1030.34RNase H domain-containing protein OS=Asparagus officinalis OX=4686 GN=A4U43_C05F... [more]
A0A5P1E8M94.1e-1033.33RNase H domain-containing protein OS=Asparagus officinalis OX=4686 GN=A4U43_C10F... [more]
A0A5P1FW406.9e-1030.66RNase H domain-containing protein OS=Asparagus officinalis OX=4686 GN=A4U43_C01F... [more]
Match NameE-valueIdentityDescription
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc08g21840.1Moc08g21840.1mRNA