Moc08g16900 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc08g16900
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
DescriptionRetrovirus-related Pol polyprotein from transposon TNT 1-94
Locationchr8: 12936537 .. 12936872 (-)
RNA-Seq ExpressionMoc08g16900
SyntenyMoc08g16900
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAGCAACCTCCTGGATATATTGAATCTGGTAAAGGGAAACTTGTTTGTCACCTTCAAAGGTCCATTTACGGACTTAAACAGTCACCAAGACAGTGGAATAAGCGATTTGACACATTTATCACCTCAAGAGGCTTCATTAAAAATTCATTTGACTGTTGCGTGTATAAGAAAACGACAAATTCTGAAGTCTCTATTTGGCTTCTACTGTATGTGGACGATATCTTGATTGCTTCAAATAGTAAGGTTGAAATAAACAAACTAAAAGCACAACTAGAAAAGAGTTTGACACGAAAGATTTGGGAGAAGTTAAAAAGATCTTGGGAATGGAAATAA

mRNA sequence

ATGAAGCAACCTCCTGGATATATTGAATCTGGTAAAGGGAAACTTGTTTGTCACCTTCAAAGGTCCATTTACGGACTTAAACAGTCACCAAGACAGTGGAATAAGCGATTTGACACATTTATCACCTCAAGAGGCTTCATTAAAAATTCATTTGACTGTTGCGTGTATAAGAAAACGACAAATTCTGAAGTCTCTATTTGGCTTCTACTGTATGTGGACGATATCTTGATTGCTTCAAATAGTAAGGTTGAAATAAACAAACTAAAAGCACAACTAGAAAAGAGTTTGACACGAAAGATTTGGGAGAAGTTAAAAAGATCTTGGGAATGGAAATAA

Coding sequence (CDS)

ATGAAGCAACCTCCTGGATATATTGAATCTGGTAAAGGGAAACTTGTTTGTCACCTTCAAAGGTCCATTTACGGACTTAAACAGTCACCAAGACAGTGGAATAAGCGATTTGACACATTTATCACCTCAAGAGGCTTCATTAAAAATTCATTTGACTGTTGCGTGTATAAGAAAACGACAAATTCTGAAGTCTCTATTTGGCTTCTACTGTATGTGGACGATATCTTGATTGCTTCAAATAGTAAGGTTGAAATAAACAAACTAAAAGCACAACTAGAAAAGAGTTTGACACGAAAGATTTGGGAGAAGTTAAAAAGATCTTGGGAATGGAAATAA

Protein sequence

MKQPPGYIESGKGKLVCHLQRSIYGLKQSPRQWNKRFDTFITSRGFIKNSFDCCVYKKTTNSEVSIWLLLYVDDILIASNSKVEINKLKAQLEKSLTRKIWEKLKRSWEWK
Homology
BLAST of Moc08g16900 vs. NCBI nr
Match: KYP38863.1 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 [Cajanus cajan])

HSP 1 Score: 121.3 bits (303), Expect = 5.2e-24
Identity = 58/99 (58.59%), Postives = 70/99 (70.71%), Query Frame = 0

Query: 1   MKQPPGYIESGKGKLVCHLQRSIYGLKQSPRQWNKRFDTFITSRGFIKNSFDCCVYKKTT 60
           MKQP G+++ GK   VC LQ+S+YGLKQSPRQW KRFDTF+ S+GF K+ +DCCVY K  
Sbjct: 1   MKQPEGFVKLGKEDYVCQLQKSLYGLKQSPRQWYKRFDTFMISKGFNKSPYDCCVYHKKV 60

Query: 61  NSEVSIWLLLYVDDILIASNSKVEINKLKAQLEKSLTRK 100
                I+LLLYVDDILIA+ +K EI  LKA L      K
Sbjct: 61  EDGFMIYLLLYVDDILIAAKNKSEIQNLKALLSGEFDMK 99

BLAST of Moc08g16900 vs. NCBI nr
Match: KAG8474589.1 (hypothetical protein CXB51_031229 [Gossypium anomalum])

HSP 1 Score: 120.6 bits (301), Expect = 8.8e-24
Identity = 58/108 (53.70%), Postives = 76/108 (70.37%), Query Frame = 0

Query: 1   MKQPPGYIESGKGKLVCHLQRSIYGLKQSPRQWNKRFDTFITSRGFIKNSFDCCVYKKTT 60
           M+QP G+  S K   VC L++S+YGLKQSPRQW KRFD+F+TS  F ++SFD CVY K  
Sbjct: 106 MQQPEGFTVSEKEDYVCLLKKSLYGLKQSPRQWYKRFDSFMTSHDFKRSSFDSCVYFKKN 165

Query: 61  NSEVSIWLLLYVDDILIASNSKVEINKLKAQLEKSLTRKIWEKLKRSW 109
           N    ++LLLYVDD+LIA+  K EI K+KAQL +    K W++ K +W
Sbjct: 166 NDGSFVYLLLYVDDMLIAAKDKGEIRKVKAQLSEEFEMKDWDQQKNTW 213

BLAST of Moc08g16900 vs. NCBI nr
Match: RLM61347.1 (retrotransposon protein, putative, Ty1-copia subclass [Panicum miliaceum])

HSP 1 Score: 120.2 bits (300), Expect = 1.2e-23
Identity = 61/99 (61.62%), Postives = 70/99 (70.71%), Query Frame = 0

Query: 1   MKQPPGYIESGKGKLVCHLQRSIYGLKQSPRQWNKRFDTFITSRGFIKNSFDCCVYKKTT 60
           M QP G+I  GK K VC L+RS+YGLKQSPRQWNKRFD+F+ S GF ++ FD CVY K  
Sbjct: 552 MDQPEGFIVPGKEKFVCELKRSLYGLKQSPRQWNKRFDSFMLSHGFKRSKFDSCVYIKHV 611

Query: 61  NSEVSIWLLLYVDDILIASNSKVEINKLKAQLEKSLTRK 100
           N    I+LLLYVDDILIA+ SKVEI KLK  L      K
Sbjct: 612 NGS-PIYLLLYVDDILIATKSKVEITKLKKLLSSEFDMK 649

BLAST of Moc08g16900 vs. NCBI nr
Match: RVW49359.1 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 [Vitis vinifera])

HSP 1 Score: 117.9 bits (294), Expect = 5.7e-23
Identity = 56/101 (55.45%), Postives = 70/101 (69.31%), Query Frame = 0

Query: 1   MKQPPGYIESGKGKLVCHLQRSIYGLKQSPRQWNKRFDTFITSRGFIKNSFDCCVYKKTT 60
           MKQP G+++S K   VC L++S+YGLKQSPRQW KRFD F+ S  FI+N +D CVY KT 
Sbjct: 326 MKQPEGFVDSEKSDHVCFLKKSLYGLKQSPRQWYKRFDAFMVSHEFIRNQYDSCVYFKTL 385

Query: 61  NSEVSIWLLLYVDDILIASNSKVEINKLKAQLEKSLTRKIW 102
                I+LLLYVDD+LIA+ ++ EINKLK  L      K W
Sbjct: 386 PDGSFIYLLLYVDDMLIAAKNRAEINKLKQLLSSEFEMKDW 426

BLAST of Moc08g16900 vs. NCBI nr
Match: KAG8478610.1 (hypothetical protein CXB51_028417 [Gossypium anomalum])

HSP 1 Score: 117.1 bits (292), Expect = 9.8e-23
Identity = 57/106 (53.77%), Postives = 75/106 (70.75%), Query Frame = 0

Query: 1   MKQPPGYIESGKGKLVCHLQRSIYGLKQSPRQWNKRFDTFITSRGFIKNSFDCCVYKKTT 60
           M+QP G+  S K   VC L++S+YGLKQSPRQW KRFD+F+TS  F ++SFD CVY K  
Sbjct: 799 MQQPEGFTVSEKEDYVCLLKKSLYGLKQSPRQWYKRFDSFMTSHDFKRSSFDSCVYFKKN 858

Query: 61  NSEVSIWLLLYVDDILIASNSKVEINKLKAQLEKSLTRKIWEKLKR 107
           N    ++LLLYVDD+LIA+  K EI K+KAQL +    K W++ +R
Sbjct: 859 NDGSFVYLLLYVDDMLIAAKDKGEIRKVKAQLSEEFEMKDWDQQRR 904

BLAST of Moc08g16900 vs. ExPASy Swiss-Prot
Match: P10978 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 94.4 bits (233), Expect = 8.9e-19
Identity = 48/99 (48.48%), Postives = 63/99 (63.64%), Query Frame = 0

Query: 1    MKQPPGYIESGKGKLVCHLQRSIYGLKQSPRQWNKRFDTFITSRGFIKNSFDCCVYKKTT 60
            M+QP G+  +GK  +VC L +S+YGLKQ+PRQW  +FD+F+ S+ ++K   D CVY K  
Sbjct: 938  MEQPEGFEVAGKKHMVCKLNKSLYGLKQAPRQWYMKFDSFMKSQTYLKTYSDPCVYFKRF 997

Query: 61   NSEVSIWLLLYVDDILIASNSKVEINKLKAQLEKSLTRK 100
            +    I LLLYVDD+LI    K  I KLK  L KS   K
Sbjct: 998  SENNFIILLLYVDDMLIVGKDKGLIAKLKGDLSKSFDMK 1036

BLAST of Moc08g16900 vs. ExPASy Swiss-Prot
Match: Q9ZT94 (Retrovirus-related Pol polyprotein from transposon RE2 OS=Arabidopsis thaliana OX=3702 GN=RE2 PE=4 SV=1)

HSP 1 Score: 70.9 bits (172), Expect = 1.1e-11
Identity = 36/104 (34.62%), Postives = 58/104 (55.77%), Query Frame = 0

Query: 1    MKQPPGYIESGKGKLVCHLQRSIYGLKQSPRQWNKRFDTFITSRGFIKNSFDCCVYKKTT 60
            M QPPG+++  +   VC L+++IYGLKQ+PR W     T++ + GF+ +  D  ++    
Sbjct: 1064 MSQPPGFVDKDRPDYVCRLRKAIYGLKQAPRAWYVELRTYLLTVGFVNSISDTSLFVLQR 1123

Query: 61   NSEVSIWLLLYVDDILIASNSKVEINKLKAQLEKSLTRKIWEKL 105
               + I++L+YVDDILI  N  V +      L +  + K  E L
Sbjct: 1124 GRSI-IYMLVYVDDILITGNDTVLLKHTLDALSQRFSVKEHEDL 1166

BLAST of Moc08g16900 vs. ExPASy Swiss-Prot
Match: Q94HW2 (Retrovirus-related Pol polyprotein from transposon RE1 OS=Arabidopsis thaliana OX=3702 GN=RE1 PE=2 SV=1)

HSP 1 Score: 68.9 bits (167), Expect = 4.0e-11
Identity = 33/104 (31.73%), Postives = 58/104 (55.77%), Query Frame = 0

Query: 1    MKQPPGYIESGKGKLVCHLQRSIYGLKQSPRQWNKRFDTFITSRGFIKNSFDCCVYKKTT 60
            M QPPG+I+  +   VC L++++YGLKQ+PR W      ++ + GF+ +  D  ++    
Sbjct: 1081 MSQPPGFIDKDRPNYVCKLRKALYGLKQAPRAWYVELRNYLLTIGFVNSVSDTSLFVLQR 1140

Query: 61   NSEVSIWLLLYVDDILIASNSKVEINKLKAQLEKSLTRKIWEKL 105
               + +++L+YVDDILI  N    ++     L +  + K  E+L
Sbjct: 1141 GKSI-VYMLVYVDDILITGNDPTLLHNTLDNLSQRFSVKDHEEL 1183

BLAST of Moc08g16900 vs. ExPASy Swiss-Prot
Match: P25600 (Putative transposon Ty5-1 protein YCL074W OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY5A PE=5 SV=2)

HSP 1 Score: 62.8 bits (151), Expect = 2.9e-09
Identity = 32/99 (32.32%), Postives = 58/99 (58.59%), Query Frame = 0

Query: 1   MKQPPGYIESGKGKLVCHLQRSIYGLKQSPRQWNKRFDTFITSRGFIKNSFDCCVYKKTT 60
           +KQPPG++       V  L   +YGLKQ+P  WN+  +  +   GF ++  +  +Y ++T
Sbjct: 18  VKQPPGFVNERNPDYVWELYGGMYGLKQAPLLWNEHINNTLKKIGFCRHEGEHGLYFRST 77

Query: 61  NSEVSIWLLLYVDDILIASNSKVEINKLKAQLEKSLTRK 100
            S+  I++ +YVDD+L+A+ S    +++K +L K  + K
Sbjct: 78  -SDGPIYIAVYVDDLLVAAPSPKIYDRVKQELTKLYSMK 115

BLAST of Moc08g16900 vs. ExPASy Swiss-Prot
Match: P04146 (Copia protein OS=Drosophila melanogaster OX=7227 GN=GIP PE=1 SV=3)

HSP 1 Score: 59.7 bits (143), Expect = 2.4e-08
Identity = 30/78 (38.46%), Postives = 46/78 (58.97%), Query Frame = 0

Query: 16   VCHLQRSIYGLKQSPRQWNKRFDTFITSRGFIKNSFDCCVY-KKTTNSEVSIWLLLYVDD 75
            VC L ++IYGLKQ+ R W + F+  +    F+ +S D C+Y     N   +I++LLYVDD
Sbjct: 1031 VCKLNKAIYGLKQAARCWFEVFEQALKECEFVNSSVDRCIYILDKGNINENIYVLLYVDD 1090

Query: 76   ILIASNSKVEINKLKAQL 93
            ++IA+     +N  K  L
Sbjct: 1091 VVIATGDMTRMNNFKRYL 1108

BLAST of Moc08g16900 vs. ExPASy TrEMBL
Match: A0A151R8N8 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Cajanus cajan OX=3821 GN=KK1_039844 PE=4 SV=1)

HSP 1 Score: 121.3 bits (303), Expect = 2.5e-24
Identity = 58/99 (58.59%), Postives = 70/99 (70.71%), Query Frame = 0

Query: 1   MKQPPGYIESGKGKLVCHLQRSIYGLKQSPRQWNKRFDTFITSRGFIKNSFDCCVYKKTT 60
           MKQP G+++ GK   VC LQ+S+YGLKQSPRQW KRFDTF+ S+GF K+ +DCCVY K  
Sbjct: 1   MKQPEGFVKLGKEDYVCQLQKSLYGLKQSPRQWYKRFDTFMISKGFNKSPYDCCVYHKKV 60

Query: 61  NSEVSIWLLLYVDDILIASNSKVEINKLKAQLEKSLTRK 100
                I+LLLYVDDILIA+ +K EI  LKA L      K
Sbjct: 61  EDGFMIYLLLYVDDILIAAKNKSEIQNLKALLSGEFDMK 99

BLAST of Moc08g16900 vs. ExPASy TrEMBL
Match: A0A3L6PRB6 (Retrotransposon protein, putative, Ty1-copia subclass OS=Panicum miliaceum OX=4540 GN=C2845_PM14G10480 PE=4 SV=1)

HSP 1 Score: 120.2 bits (300), Expect = 5.6e-24
Identity = 61/99 (61.62%), Postives = 70/99 (70.71%), Query Frame = 0

Query: 1   MKQPPGYIESGKGKLVCHLQRSIYGLKQSPRQWNKRFDTFITSRGFIKNSFDCCVYKKTT 60
           M QP G+I  GK K VC L+RS+YGLKQSPRQWNKRFD+F+ S GF ++ FD CVY K  
Sbjct: 552 MDQPEGFIVPGKEKFVCELKRSLYGLKQSPRQWNKRFDSFMLSHGFKRSKFDSCVYIKHV 611

Query: 61  NSEVSIWLLLYVDDILIASNSKVEINKLKAQLEKSLTRK 100
           N    I+LLLYVDDILIA+ SKVEI KLK  L      K
Sbjct: 612 NGS-PIYLLLYVDDILIATKSKVEITKLKKLLSSEFDMK 649

BLAST of Moc08g16900 vs. ExPASy TrEMBL
Match: A0A438ENN9 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Vitis vinifera OX=29760 GN=POLX_2814 PE=4 SV=1)

HSP 1 Score: 117.9 bits (294), Expect = 2.8e-23
Identity = 56/101 (55.45%), Postives = 70/101 (69.31%), Query Frame = 0

Query: 1   MKQPPGYIESGKGKLVCHLQRSIYGLKQSPRQWNKRFDTFITSRGFIKNSFDCCVYKKTT 60
           MKQP G+++S K   VC L++S+YGLKQSPRQW KRFD F+ S  FI+N +D CVY KT 
Sbjct: 326 MKQPEGFVDSEKSDHVCFLKKSLYGLKQSPRQWYKRFDAFMVSHEFIRNQYDSCVYFKTL 385

Query: 61  NSEVSIWLLLYVDDILIASNSKVEINKLKAQLEKSLTRKIW 102
                I+LLLYVDD+LIA+ ++ EINKLK  L      K W
Sbjct: 386 PDGSFIYLLLYVDDMLIAAKNRAEINKLKQLLSSEFEMKDW 426

BLAST of Moc08g16900 vs. ExPASy TrEMBL
Match: A0A5H2Y1B3 (Transposable element protein OS=Prunus dulcis OX=3755 GN=Prudu_439S000200 PE=4 SV=1)

HSP 1 Score: 116.3 bits (290), Expect = 8.1e-23
Identity = 58/111 (52.25%), Postives = 72/111 (64.86%), Query Frame = 0

Query: 1   MKQPPGYIESGKGKLVCHLQRSIYGLKQSPRQWNKRFDTFITSRGFIKNSFDCCVYKKTT 60
           M QP G+  +GK   VC LQ+S+YGLKQSPRQW KRFD F+  + + ++ +D CVY +  
Sbjct: 598 MSQPEGFKVAGKENWVCKLQKSLYGLKQSPRQWYKRFDRFMIGQKYTRSHYDHCVYFRKL 657

Query: 61  NSEVSIWLLLYVDDILIASNSKVEINKLKAQLEKSLTRKIWEKLKRSWEWK 112
                I+LLLYVDD+LIA  SKVEI +LK QL      K  EKL R W WK
Sbjct: 658 QDGTFIYLLLYVDDMLIACKSKVEIERLKTQLSNEFEMKDLEKLGRYWVWK 708

BLAST of Moc08g16900 vs. ExPASy TrEMBL
Match: A0A438EBT7 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Vitis vinifera OX=29760 GN=POLX_3558 PE=4 SV=1)

HSP 1 Score: 115.2 bits (287), Expect = 1.8e-22
Identity = 54/99 (54.55%), Postives = 69/99 (69.70%), Query Frame = 0

Query: 1    MKQPPGYIESGKGKLVCHLQRSIYGLKQSPRQWNKRFDTFITSRGFIKNSFDCCVYKKTT 60
            MKQP G+++S K   VC L++S+YGLKQSPRQW KRFD F+ S  F++N +D CVY KT 
Sbjct: 916  MKQPEGFVDSEKSDHVCFLKKSLYGLKQSPRQWYKRFDAFMVSHEFVRNQYDSCVYFKTL 975

Query: 61   NSEVSIWLLLYVDDILIASNSKVEINKLKAQLEKSLTRK 100
                 I+LLLYVDD+LIA+ ++ EINKLK  L      K
Sbjct: 976  PDSSFIYLLLYVDDMLIAAKNRAEINKLKQLLSSEFEMK 1014

BLAST of Moc08g16900 vs. TAIR 10
Match: AT4G23160.1 (cysteine-rich RLK (RECEPTOR-like protein kinase) 8 )

HSP 1 Score: 73.9 bits (180), Expect = 8.8e-14
Identity = 39/97 (40.21%), Postives = 62/97 (63.92%), Query Frame = 0

Query: 1   MKQPPGYI----ESGKGKLVCHLQRSIYGLKQSPRQWNKRFDTFITSRGFIKNSFDCCVY 60
           MK PPGY     +S     VC+L++SIYGLKQ+ RQW  +F   +   GF+++  D   +
Sbjct: 210 MKLPPGYAARQGDSLPPNAVCYLKKSIYGLKQASRQWFLKFSVTLIGFGFVQSHSDHTYF 269

Query: 61  KKTTNSEVSIWLLLYVDDILIASNSKVEINKLKAQLE 94
            K T + + + +L+YVDDI+I SN+   +++LK+QL+
Sbjct: 270 LKIT-ATLFLCVLVYVDDIIICSNNDAAVDELKSQLK 305

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KYP38863.15.2e-2458.59Retrovirus-related Pol polyprotein from transposon TNT 1-94 [Cajanus cajan][more]
KAG8474589.18.8e-2453.70hypothetical protein CXB51_031229 [Gossypium anomalum][more]
RLM61347.11.2e-2361.62retrotransposon protein, putative, Ty1-copia subclass [Panicum miliaceum][more]
RVW49359.15.7e-2355.45Retrovirus-related Pol polyprotein from transposon TNT 1-94 [Vitis vinifera][more]
KAG8478610.19.8e-2353.77hypothetical protein CXB51_028417 [Gossypium anomalum][more]
Match NameE-valueIdentityDescription
P109788.9e-1948.48Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Nicotiana tabacum... [more]
Q9ZT941.1e-1134.62Retrovirus-related Pol polyprotein from transposon RE2 OS=Arabidopsis thaliana O... [more]
Q94HW24.0e-1131.73Retrovirus-related Pol polyprotein from transposon RE1 OS=Arabidopsis thaliana O... [more]
P256002.9e-0932.32Putative transposon Ty5-1 protein YCL074W OS=Saccharomyces cerevisiae (strain AT... [more]
P041462.4e-0838.46Copia protein OS=Drosophila melanogaster OX=7227 GN=GIP PE=1 SV=3[more]
Match NameE-valueIdentityDescription
A0A151R8N82.5e-2458.59Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Cajanus cajan OX=... [more]
A0A3L6PRB65.6e-2461.62Retrotransposon protein, putative, Ty1-copia subclass OS=Panicum miliaceum OX=45... [more]
A0A438ENN92.8e-2355.45Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Vitis vinifera OX... [more]
A0A5H2Y1B38.1e-2352.25Transposable element protein OS=Prunus dulcis OX=3755 GN=Prudu_439S000200 PE=4 S... [more]
A0A438EBT71.8e-2254.55Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Vitis vinifera OX... [more]
Match NameE-valueIdentityDescription
AT4G23160.18.8e-1440.21cysteine-rich RLK (RECEPTOR-like protein kinase) 8 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013103Reverse transcriptase, RNA-dependent DNA polymerasePFAMPF07727RVT_2coord: 1..99
e-value: 1.4E-19
score: 70.7
NoneNo IPR availablePANTHERPTHR11439GAG-POL-RELATED RETROTRANSPOSONcoord: 1..93
NoneNo IPR availablePANTHERPTHR11439:SF322CLASSICAL ARABINOGALACTAN PROTEIN 9-LIKEcoord: 1..93
IPR043502DNA/RNA polymerase superfamilySUPERFAMILY56672DNA/RNA polymerasescoord: 15..96

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc08g16900.1Moc08g16900.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005488 binding