Moc08g15850 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc08g15850
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
DescriptionCCHC-type domain-containing protein
Locationchr8: 12189222 .. 12189500 (+)
RNA-Seq ExpressionMoc08g15850
SyntenyMoc08g15850
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGACTGAGCACATCAACACATTGAATACTCTGTTTTCTCAACTCACATCATTGGGTTGTAAAATAGAACCAAAAGAACGTGCTGAACTTCTACTTCAAAGTCTTCCCGATTCGTATGATCAACTTGTCATCAACCTGATAAATAATGTTCTCACCGACTATCTAAACTTGGAAGATATTGGATCTGATGTCCTACAAGAGGAAAATCGGCGCAAGAACAAAGTAGATAAGTTGGGCGAGATCACAACAAGTAGAGGCTCTAGCAGTGACAAGAGATAG

mRNA sequence

ATGACTGAGCACATCAACACATTGAATACTCTGTTTTCTCAACTCACATCATTGGGTTGTAAAATAGAACCAAAAGAACGTGCTGAACTTCTACTTCAAAGTCTTCCCGATTCGTATGATCAACTTGTCATCAACCTGATAAATAATGTTCTCACCGACTATCTAAACTTGGAAGATATTGGATCTGATGTCCTACAAGAGGAAAATCGGCGCAAGAACAAAGTAGATAAGTTGGGCGAGATCACAACAAGTAGAGGCTCTAGCAGTGACAAGAGATAG

Coding sequence (CDS)

ATGACTGAGCACATCAACACATTGAATACTCTGTTTTCTCAACTCACATCATTGGGTTGTAAAATAGAACCAAAAGAACGTGCTGAACTTCTACTTCAAAGTCTTCCCGATTCGTATGATCAACTTGTCATCAACCTGATAAATAATGTTCTCACCGACTATCTAAACTTGGAAGATATTGGATCTGATGTCCTACAAGAGGAAAATCGGCGCAAGAACAAAGTAGATAAGTTGGGCGAGATCACAACAAGTAGAGGCTCTAGCAGTGACAAGAGATAG

Protein sequence

MTEHINTLNTLFSQLTSLGCKIEPKERAELLLQSLPDSYDQLVINLINNVLTDYLNLEDIGSDVLQEENRRKNKVDKLGEITTSRGSSSDKR
Homology
BLAST of Moc08g15850 vs. NCBI nr
Match: BAD34493.1 (Gag-Pol [Ipomoea batatas])

HSP 1 Score: 122.9 bits (307), Expect = 1.5e-24
Identity = 63/97 (64.95%), Postives = 78/97 (80.41%), Query Frame = 0

Query: 1   MTEHINTLNTLFSQLTSLGCKIEPKERAELLLQSLPDSYDQLVINLINNVLTDYLNLEDI 60
           +TEH+NTLNTLFSQLTSL CKIEP+ERAELLLQSLPDSYDQL+INL NN+LTDYL  +D+
Sbjct: 114 VTEHLNTLNTLFSQLTSLSCKIEPQERAELLLQSLPDSYDQLIINLTNNILTDYLVFDDV 173

Query: 61  GSDVLQEENRRKNKVDK------LGEITTSRGSSSDK 92
            + VL+EE+RRKNK D+         +T  RG S+++
Sbjct: 174 AAAVLEEESRRKNKEDRQVNLQQAEALTVMRGRSTER 210

BLAST of Moc08g15850 vs. NCBI nr
Match: KAA0061179.1 (Gag-Pol [Cucumis melo var. makuwa] >TYK06231.1 Gag-Pol [Cucumis melo var. makuwa])

HSP 1 Score: 121.7 bits (304), Expect = 3.3e-24
Identity = 66/96 (68.75%), Postives = 75/96 (78.12%), Query Frame = 0

Query: 1   MTEHINTLNTLFSQLTSLGCKIEPKERAELLLQSLPDSYDQLVINLINNVLTDYLNLEDI 60
           MTEH+N LNTLFSQLT L  KIEP ERAELLLQSLPDSYDQLVINL NN+LTDYL+ +D+
Sbjct: 114 MTEHMNILNTLFSQLTLLAYKIEPNERAELLLQSLPDSYDQLVINLTNNILTDYLSFDDV 173

Query: 61  GSDVLQEENRRKNKVDKL------GEITTSRGSSSD 91
            S VL+EENRRKNK DKL        +  +RG SS+
Sbjct: 174 TSAVLEEENRRKNKEDKLVSSQQAEALVVTRGRSSE 209

BLAST of Moc08g15850 vs. NCBI nr
Match: KAA0044949.1 (hypothetical protein E6C27_scaffold74G002510 [Cucumis melo var. makuwa])

HSP 1 Score: 119.8 bits (299), Expect = 1.3e-23
Identity = 60/78 (76.92%), Postives = 68/78 (87.18%), Query Frame = 0

Query: 1   MTEHINTLNTLFSQLTSLGCKIEPKERAELLLQSLPDSYDQLVINLINNVLTDYLNLEDI 60
           MTEH+NTLNTLFSQLT LG KIEP + AELLLQSLPDSYDQLVINL NN+LTDYL+ +D+
Sbjct: 114 MTEHMNTLNTLFSQLTLLGYKIEPNKHAELLLQSLPDSYDQLVINLTNNILTDYLSFDDV 173

Query: 61  GSDVLQEENRRKNKVDKL 79
            + VL+EENR KNK DKL
Sbjct: 174 AATVLEEENRCKNKEDKL 191

BLAST of Moc08g15850 vs. NCBI nr
Match: TYK16527.1 (hypothetical protein E5676_scaffold21G003420 [Cucumis melo var. makuwa])

HSP 1 Score: 117.5 bits (293), Expect = 6.2e-23
Identity = 60/78 (76.92%), Postives = 68/78 (87.18%), Query Frame = 0

Query: 1   MTEHINTLNTLFSQLTSLGCKIEPKERAELLLQSLPDSYDQLVINLINNVLTDYLNLEDI 60
           MTEH+NTLNTLFSQLT LG KIEP + AELLLQSLPDSYDQLVINL NN+LTDYL+ +D+
Sbjct: 114 MTEHMNTLNTLFSQLTLLGYKIEPNKCAELLLQSLPDSYDQLVINLTNNILTDYLSFDDV 173

Query: 61  GSDVLQEENRRKNKVDKL 79
            + VL+EENR KNK DKL
Sbjct: 174 VATVLEEENRCKNKEDKL 191

BLAST of Moc08g15850 vs. NCBI nr
Match: KAA0026163.1 (Gag-Pol [Cucumis melo var. makuwa] >TYK11620.1 Gag-Pol [Cucumis melo var. makuwa])

HSP 1 Score: 117.1 bits (292), Expect = 8.1e-23
Identity = 61/78 (78.21%), Postives = 67/78 (85.90%), Query Frame = 0

Query: 1   MTEHINTLNTLFSQLTSLGCKIEPKERAELLLQSLPDSYDQLVINLINNVLTDYLNLEDI 60
           MTEH+NTLNTLFSQL  LG KIEP ERAELLLQSL DSYDQLVINL NN+L DYL+ +D+
Sbjct: 114 MTEHMNTLNTLFSQLALLGYKIEPNERAELLLQSLLDSYDQLVINLKNNILIDYLSFDDV 173

Query: 61  GSDVLQEENRRKNKVDKL 79
            S VL+EENRRKNK DKL
Sbjct: 174 VSAVLEEENRRKNKEDKL 191

BLAST of Moc08g15850 vs. ExPASy Swiss-Prot
Match: P10978 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 57.4 bits (137), Expect = 1.0e-07
Identity = 37/87 (42.53%), Postives = 52/87 (59.77%), Query Frame = 0

Query: 4   HINTLNTLFSQLTSLGCKIEPKERAELLLQSLPDSYDQLVINLINNVLTDYLNLEDIGSD 63
           H+N  N L +QL +LG KIE +++A LLL SLP SYD L   +++   T  + L+D+ S 
Sbjct: 121 HLNVFNGLITQLANLGVKIEEEDKAILLLNSLPSSYDNLATTILHGKTT--IELKDVTSA 180

Query: 64  VLQEENRRKNKVDKLGE--ITTSRGSS 89
           +L  E  RK K +  G+  IT  RG S
Sbjct: 181 LLLNEKMRK-KPENQGQALITEGRGRS 204

BLAST of Moc08g15850 vs. ExPASy TrEMBL
Match: Q6BCY1 (Gag-Pol OS=Ipomoea batatas OX=4120 GN=Rtsp-1AA PE=4 SV=1)

HSP 1 Score: 122.9 bits (307), Expect = 7.2e-25
Identity = 63/97 (64.95%), Postives = 78/97 (80.41%), Query Frame = 0

Query: 1   MTEHINTLNTLFSQLTSLGCKIEPKERAELLLQSLPDSYDQLVINLINNVLTDYLNLEDI 60
           +TEH+NTLNTLFSQLTSL CKIEP+ERAELLLQSLPDSYDQL+INL NN+LTDYL  +D+
Sbjct: 114 VTEHLNTLNTLFSQLTSLSCKIEPQERAELLLQSLPDSYDQLIINLTNNILTDYLVFDDV 173

Query: 61  GSDVLQEENRRKNKVDK------LGEITTSRGSSSDK 92
            + VL+EE+RRKNK D+         +T  RG S+++
Sbjct: 174 AAAVLEEESRRKNKEDRQVNLQQAEALTVMRGRSTER 210

BLAST of Moc08g15850 vs. ExPASy TrEMBL
Match: A0A5A7V644 (Gag-Pol OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold157G00020 PE=4 SV=1)

HSP 1 Score: 121.7 bits (304), Expect = 1.6e-24
Identity = 66/96 (68.75%), Postives = 75/96 (78.12%), Query Frame = 0

Query: 1   MTEHINTLNTLFSQLTSLGCKIEPKERAELLLQSLPDSYDQLVINLINNVLTDYLNLEDI 60
           MTEH+N LNTLFSQLT L  KIEP ERAELLLQSLPDSYDQLVINL NN+LTDYL+ +D+
Sbjct: 114 MTEHMNILNTLFSQLTLLAYKIEPNERAELLLQSLPDSYDQLVINLTNNILTDYLSFDDV 173

Query: 61  GSDVLQEENRRKNKVDKL------GEITTSRGSSSD 91
            S VL+EENRRKNK DKL        +  +RG SS+
Sbjct: 174 TSAVLEEENRRKNKEDKLVSSQQAEALVVTRGRSSE 209

BLAST of Moc08g15850 vs. ExPASy TrEMBL
Match: A0A5A7TUN0 (Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold74G002510 PE=4 SV=1)

HSP 1 Score: 119.8 bits (299), Expect = 6.1e-24
Identity = 60/78 (76.92%), Postives = 68/78 (87.18%), Query Frame = 0

Query: 1   MTEHINTLNTLFSQLTSLGCKIEPKERAELLLQSLPDSYDQLVINLINNVLTDYLNLEDI 60
           MTEH+NTLNTLFSQLT LG KIEP + AELLLQSLPDSYDQLVINL NN+LTDYL+ +D+
Sbjct: 114 MTEHMNTLNTLFSQLTLLGYKIEPNKHAELLLQSLPDSYDQLVINLTNNILTDYLSFDDV 173

Query: 61  GSDVLQEENRRKNKVDKL 79
            + VL+EENR KNK DKL
Sbjct: 174 AATVLEEENRCKNKEDKL 191

BLAST of Moc08g15850 vs. ExPASy TrEMBL
Match: A0A5D3CXA6 (Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold21G003420 PE=4 SV=1)

HSP 1 Score: 117.5 bits (293), Expect = 3.0e-23
Identity = 60/78 (76.92%), Postives = 68/78 (87.18%), Query Frame = 0

Query: 1   MTEHINTLNTLFSQLTSLGCKIEPKERAELLLQSLPDSYDQLVINLINNVLTDYLNLEDI 60
           MTEH+NTLNTLFSQLT LG KIEP + AELLLQSLPDSYDQLVINL NN+LTDYL+ +D+
Sbjct: 114 MTEHMNTLNTLFSQLTLLGYKIEPNKCAELLLQSLPDSYDQLVINLTNNILTDYLSFDDV 173

Query: 61  GSDVLQEENRRKNKVDKL 79
            + VL+EENR KNK DKL
Sbjct: 174 VATVLEEENRCKNKEDKL 191

BLAST of Moc08g15850 vs. ExPASy TrEMBL
Match: A0A5A7SNG9 (Gag-Pol OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold263G00680 PE=4 SV=1)

HSP 1 Score: 117.1 bits (292), Expect = 3.9e-23
Identity = 61/78 (78.21%), Postives = 67/78 (85.90%), Query Frame = 0

Query: 1   MTEHINTLNTLFSQLTSLGCKIEPKERAELLLQSLPDSYDQLVINLINNVLTDYLNLEDI 60
           MTEH+NTLNTLFSQL  LG KIEP ERAELLLQSL DSYDQLVINL NN+L DYL+ +D+
Sbjct: 114 MTEHMNTLNTLFSQLALLGYKIEPNERAELLLQSLLDSYDQLVINLKNNILIDYLSFDDV 173

Query: 61  GSDVLQEENRRKNKVDKL 79
            S VL+EENRRKNK DKL
Sbjct: 174 VSAVLEEENRRKNKEDKL 191

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
BAD34493.11.5e-2464.95Gag-Pol [Ipomoea batatas][more]
KAA0061179.13.3e-2468.75Gag-Pol [Cucumis melo var. makuwa] >TYK06231.1 Gag-Pol [Cucumis melo var. makuwa... [more]
KAA0044949.11.3e-2376.92hypothetical protein E6C27_scaffold74G002510 [Cucumis melo var. makuwa][more]
TYK16527.16.2e-2376.92hypothetical protein E5676_scaffold21G003420 [Cucumis melo var. makuwa][more]
KAA0026163.18.1e-2378.21Gag-Pol [Cucumis melo var. makuwa] >TYK11620.1 Gag-Pol [Cucumis melo var. makuwa... [more]
Match NameE-valueIdentityDescription
P109781.0e-0742.53Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Nicotiana tabacum... [more]
Match NameE-valueIdentityDescription
Q6BCY17.2e-2564.95Gag-Pol OS=Ipomoea batatas OX=4120 GN=Rtsp-1AA PE=4 SV=1[more]
A0A5A7V6441.6e-2468.75Gag-Pol OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold157G00020 PE=4 S... [more]
A0A5A7TUN06.1e-2476.92Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold... [more]
A0A5D3CXA63.0e-2376.92Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold... [more]
A0A5A7SNG93.9e-2378.21Gag-Pol OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold263G00680 PE=4 S... [more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePFAMPF14223Retrotran_gag_2coord: 1..74
e-value: 3.6E-15
score: 56.0
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 69..92

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc08g15850.1Moc08g15850.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0015074 DNA integration
biological_process GO:0016310 phosphorylation
molecular_function GO:0016301 kinase activity
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0000166 nucleotide binding
molecular_function GO:0008270 zinc ion binding