Moc08g12310 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc08g12310
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	Peptide chain release factor PrfB1
Location	chr8: 9152321 .. 9156426 (+)
RNA-Seq Expression	Moc08g12310
Synteny	Moc08g12310

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCCGTTCTTCTGCGCCCACTGTGGCTGTGCGGCTCCTTCTCGCTCGTCTTCCCATTTCCCAAATTTCAATCCACAAGAACTTCACTCTCGCTAGAATTCCCACCGCCACCGTCCTCCCCATCTCCTCACTTTCCCACTTCTCTCTCAATTCGCTCTCGTTTTTCCATTGGTGAGTCTTGATTCGAAGATTTCCCCACTTCTAATCCTTCTGTATCCACTTTATTAGCTCTTGTTTGAGTTTTTTTAATCGAATTGATTCTGGGTTATTCTTAATTTTTTTTTGAATTGTTAGTTTTTTTTTTCATGTTTAATTCTTCTTCCTCGTTGTTGTGATAAATTCCGGTTTCTGAAACATGTTGGTTTCTTCCGGCAGTTTCATTTGCGACACCCGAGAGCCAGGTGGGCGAGGAGCTCGATGCTGATACTCGAGAATGGGCGATGCAAGGTATGTTTAGTTCTCTTACAAACAATATTTAAATTCCTAGATTGAAACTACCGTGTTCATGGGGTTTTAACTATTGAACAGCCTTTGTTGTCCTTATGTTCTTTGATATTTATTACTTAATTAACAAACGCGTTCCGAGTGTGAAGGTCTCAAAAGCAAAACTAACAATAACTGGCAGTGGGCAGTGGGCAGATACTTGAAAATTCTTTAAGGACGAATGTTTGTGATTCTAACAATAGTTTAACGTAGAAAACATTAATTTGTTACCAGGTATCCAACATTTTCGGTTGTTTTAAGGAGATGATGACATTATCTTTTCTAATATGATATATTGCCTTTTTTTTCAATGAAAAATTAAGCTTCGAGTTTTGCTGAAAAACCAAGCGTTCGTTAAATTAAAACAATATAATGTATTTGATCTCACTGCCCAAGTGATCAAATAAATATTCAAGTCTCATGTTTGGCTTCAGACGTGAAGCTTTTCCGAGTGTTAAAATTTGGTTATTAAGAGGTCACCAATGGTTCTTTGAATGATTGGTTAACCATCTTGATGGGGTGAAAAAAGAATAAGTGACTTACTAGTTGCATCCATTCATCAAACGTGGTCAATGTTTATGTTATACAATGTTCAGTTTCAAATGCTGCCATATGAATCTTCACATGAATGATAATTGATAAATTGCTTGAGCCTGTTTATCGTGTCTGTTCTGGTAATCTGGCAACTAGTGAATAACATCCCTTAAAATATTTATCGCGGCTACTTTTAGGACCCCTGCAGGCTGTTTTGTTGTCCCAGATTTTGATTTTACAAGTCAACTGTACTGATCGATGCATCTGATTCCCATTTATCGATTTTGGATTCAATGATGTTTGCAACACAATGCAAATGATATAACCAGTGCTTGTTGTTGCTGACAGATTTTTATTCCTTAAGGAAAGAAGTGGAAACAACCTCAGAGCGTGTGGAAGAGATAAGAAACTCTGCTGGGTTGTTTCAGTTGGATCAAGAACTTGCAGATTTGGAGTCGAAAGCGGCCGACAATGCTTTGTGGGATGACAGATCTAAGGCTCAACAAGTTCTTGTGGCCCTCACTGATGTGAAAGATAAAATAAAGATGCTTACTGATTTCAAAACACAGGTAGTGTATATAGCATGCAATTTAATCTTTAGAGAATACTCCATTTCATTTGCTAAAGTCACTTATGTAGACATATATGGAAGCCCTAAATTGAAAGAGTCTAAAATTATAGTAAATGATAAATGACTAATAAAGCCTTGGATCTTAGGCATCTACATCAATTTACATAATAATATGCATGTTGTAACAATCACCTCAAGTTGGAGCAAATATTAATCATGCCTAACTTGCTACAAAGCTACTCTATTCATACCCCATTTAAAGATTTTGTTATCAATATCTCCTGCTAGTTCTCCGATCTTTATATATCATGTAGACACCACTTCTATTTGCTTACAAGTAAAATGACAATTAACTTCCGTATATGTAGTTTGCATACATAAGTGCAAGTGTTTGTTGGATGGCTGTCTTAAGGTCATAGTTCACCTAAGTATAACATGGCTATGGCCTACTCTGTATTTTAGTTGGAATTGCAATATTTGATTTTAAGGCCTTTCCATTGGTCCTGTGGTCATGTAGAGAATGTTCTTTTTGTCTATTCATTTTCTTTTATGAGCAATACAATGGAAAAGGGGACTCTATACTTCTTTTTGGAACAGTACCCTTCAGAGGGTAGAATAAAGCTTGGAAGTAAGCTATTTTAAGATAATGAAACTTGAACTGTATATCTAGCTTGGAAGTAACCAAACTAATCCGTAGAAGCCAATTGGATAAATAGCCTATCCAAGTTTTTGAAAAGTGAATGAAATCATCATCTCAGGTGTGGGTTGCGTTATGATTGATTCAGGTCGAAGAAGCAGAAACAATAGTTAAACTGACTGAAGAGATGGACTCTGTAGATACTGGGCTTCTTGAAGAAGCTGCTAGTATTATCAAAGACCTAAACAAGGCACTGGATCAATTTGAATTGACTCAACTTCTTTCTGGTCCGTATGACAAAGAAGGCGCTGTCATCTCTATCTCGGCTGGAGCTGGGGGTACTGATGCACAGGTAAATAGTTAAGGTCTTATTGTGGAAACTGTTTTCAAAATAATTTTTTAGTTTTGTGTTCAAAACTGCTTCCAAGAAGTATGGTTGCGTGTGCATTTTTAAACTTCCAACTTTTTCACTTACTTCTTCGATATTAAATTTTTGATAATCTCCAGTGCAGGATTGGGCTGACATGCTTCTGAGGATGTATGTGAGATGGGGAGAGAAACAAAGATACAAAACAAGAGTGGTCGAGAAATCTGCAGGAGAGGAAGCTGGAATAAAATCAGCTACAATTGAAATCGAGGGTCGATATGCTTATGGATATATATCTGGAGAGAAAGGAACACACCGGATAGTGCGACAGTCTCCTTTTAACTCGAAAGGACTTCGACAGGTAATTATAACACTGCCCCCTGGTATCCCTACTACTTGGAGTTATCAAGGAAAGATGGACGATAGAGGGTGCATAATGTCATTATGTTTTAAACTTCTTATGAACTGTAAACTTAAGGTGGTTTTGAGCCTTCCATACCGAGTTTAGAATCTAGGGTCGACTTCTAATGTATGATGCTGCTTCCTTGGGATAGATTGTTTGATGGATGAACCTCTCTATTTTAATACGAGTTCTATGCAGACAAGTTTTTCTGGTGTGGAAGTCATGCCCCTCCTTCCCGACGAGTCCATGGATGTTGAATTACCAGAAGAGGACCTGGAAATAAGTTTTTCAAGGGCAGGAGGAAAAGGAGGTCAAAATGTTAACAAGGTTGAAACGGCTGTTCGCATTACTCATATCCCAACAGGCGTCACGGTTCGCTGCACAGGTCTTCTATACTCATTAATCATCATTGTTTATGCCACCATGAAAACTTGATGTCTGTTCTGTTCGTAAGAGTTATTGTGTCCTCAATTTTTAATCAAAATTGTTCAAAGCCACCACCACTTTTAGCAGCATAATTTCTCCTCAAGTTGTTGATGGAAATGTAATGTTATCATGGTTGGAGTATTCATTTTCTCTGAGAATACATTTCATCCAAATGTTTCTGGAAATGTTTGGCATTTGCTTGCTACAATTTACCATCTTTAAAATCTTTGGATTAGATACACGCAACCCGGGACCATTCGTACCGAGTGGACCATATATGTTTTCATTGACAATCATTGACAACAAACCTTTCAAACGTCCCCATGTTGCATGGTTTTAAACTGATGGTATTTCATTCATCTTCTCAACTATAATGCAGAGGAGAGATCACAGCTAGCAAACAAGATCAAGGCGTTAAGCCGATTGAAGGCCAAACTCTTAGTCATAGCAGAGGAGCAACGTGCATCGGAAATCAAGCAAATACGAGGAGATGCCGTAAAGGCCGAGTGGGGGCAGCAAATAAGGAACTATGTGTTTCATCCATACAAGCTTGTGAAAGATGTTAGGACAGGATATGAGACATCAGACATTGTCTCTGTAATGGATGGTGAATTGGAGCCCTTCATCAAAGCTTACCTGAAATACAAATACAGTATGGCATTGTCTACTGGTGGGGTTAACTAA

mRNA sequence

ATGGCCGTTCTTCTGCGCCCACTGTGGCTGTGCGGCTCCTTCTCGCTCGTCTTCCCATTTCCCAAATTTCAATCCACAAGAACTTCACTCTCGCTAGAATTCCCACCGCCACCGTCCTCCCCATCTCCTCACTTTCCCACTTCTCTCTCAATTCGCTCTCGTTTTTCCATTGTTTCATTTGCGACACCCGAGAGCCAGGTGGGCGAGGAGCTCGATGCTGATACTCGAGAATGGGCGATGCAAGATTTTTATTCCTTAAGGAAAGAAGTGGAAACAACCTCAGAGCGTGTGGAAGAGATAAGAAACTCTGCTGGGTTGTTTCAGTTGGATCAAGAACTTGCAGATTTGGAGTCGAAAGCGGCCGACAATGCTTTGTGGGATGACAGATCTAAGGCTCAACAAGTTCTTGTGGCCCTCACTGATGTGAAAGATAAAATAAAGATGCTTACTGATTTCAAAACACAGGTCGAAGAAGCAGAAACAATAGTTAAACTGACTGAAGAGATGGACTCTGTAGATACTGGGCTTCTTGAAGAAGCTGCTAGTATTATCAAAGACCTAAACAAGGCACTGGATCAATTTGAATTGACTCAACTTCTTTCTGGTCCGTATGACAAAGAAGGCGCTGTCATCTCTATCTCGGCTGGAGCTGGGGGTACTGATGCACAGGATTGGGCTGACATGCTTCTGAGGATGTATGTGAGATGGGGAGAGAAACAAAGATACAAAACAAGAGTGGTCGAGAAATCTGCAGGAGAGGAAGCTGGAATAAAATCAGCTACAATTGAAATCGAGGGTCGATATGCTTATGGATATATATCTGGAGAGAAAGGAACACACCGGATAGTGCGACAGTCTCCTTTTAACTCGAAAGGACTTCGACAGACAAGTTTTTCTGGTGTGGAAGTCATGCCCCTCCTTCCCGACGAGTCCATGGATGTTGAATTACCAGAAGAGGACCTGGAAATAAGTTTTTCAAGGGCAGGAGGAAAAGGAGGTCAAAATGTTAACAAGGTTGAAACGGCTGTTCGCATTACTCATATCCCAACAGGCGTCACGGTTCGCTGCACAGAGGAGAGATCACAGCTAGCAAACAAGATCAAGGCGTTAAGCCGATTGAAGGCCAAACTCTTAGTCATAGCAGAGGAGCAACGTGCATCGGAAATCAAGCAAATACGAGGAGATGCCGTAAAGGCCGAGTGGGGGCAGCAAATAAGGAACTATGTGTTTCATCCATACAAGCTTGTGAAAGATGTTAGGACAGGATATGAGACATCAGACATTGTCTCTGTAATGGATGGTGAATTGGAGCCCTTCATCAAAGCTTACCTGAAATACAAATACAGTATGGCATTGTCTACTGGTGGGGTTAACTAA

Coding sequence (CDS)

ATGGCCGTTCTTCTGCGCCCACTGTGGCTGTGCGGCTCCTTCTCGCTCGTCTTCCCATTTCCCAAATTTCAATCCACAAGAACTTCACTCTCGCTAGAATTCCCACCGCCACCGTCCTCCCCATCTCCTCACTTTCCCACTTCTCTCTCAATTCGCTCTCGTTTTTCCATTGTTTCATTTGCGACACCCGAGAGCCAGGTGGGCGAGGAGCTCGATGCTGATACTCGAGAATGGGCGATGCAAGATTTTTATTCCTTAAGGAAAGAAGTGGAAACAACCTCAGAGCGTGTGGAAGAGATAAGAAACTCTGCTGGGTTGTTTCAGTTGGATCAAGAACTTGCAGATTTGGAGTCGAAAGCGGCCGACAATGCTTTGTGGGATGACAGATCTAAGGCTCAACAAGTTCTTGTGGCCCTCACTGATGTGAAAGATAAAATAAAGATGCTTACTGATTTCAAAACACAGGTCGAAGAAGCAGAAACAATAGTTAAACTGACTGAAGAGATGGACTCTGTAGATACTGGGCTTCTTGAAGAAGCTGCTAGTATTATCAAAGACCTAAACAAGGCACTGGATCAATTTGAATTGACTCAACTTCTTTCTGGTCCGTATGACAAAGAAGGCGCTGTCATCTCTATCTCGGCTGGAGCTGGGGGTACTGATGCACAGGATTGGGCTGACATGCTTCTGAGGATGTATGTGAGATGGGGAGAGAAACAAAGATACAAAACAAGAGTGGTCGAGAAATCTGCAGGAGAGGAAGCTGGAATAAAATCAGCTACAATTGAAATCGAGGGTCGATATGCTTATGGATATATATCTGGAGAGAAAGGAACACACCGGATAGTGCGACAGTCTCCTTTTAACTCGAAAGGACTTCGACAGACAAGTTTTTCTGGTGTGGAAGTCATGCCCCTCCTTCCCGACGAGTCCATGGATGTTGAATTACCAGAAGAGGACCTGGAAATAAGTTTTTCAAGGGCAGGAGGAAAAGGAGGTCAAAATGTTAACAAGGTTGAAACGGCTGTTCGCATTACTCATATCCCAACAGGCGTCACGGTTCGCTGCACAGAGGAGAGATCACAGCTAGCAAACAAGATCAAGGCGTTAAGCCGATTGAAGGCCAAACTCTTAGTCATAGCAGAGGAGCAACGTGCATCGGAAATCAAGCAAATACGAGGAGATGCCGTAAAGGCCGAGTGGGGGCAGCAAATAAGGAACTATGTGTTTCATCCATACAAGCTTGTGAAAGATGTTAGGACAGGATATGAGACATCAGACATTGTCTCTGTAATGGATGGTGAATTGGAGCCCTTCATCAAAGCTTACCTGAAATACAAATACAGTATGGCATTGTCTACTGGTGGGGTTAACTAA

Protein sequence

MAVLLRPLWLCGSFSLVFPFPKFQSTRTSLSLEFPPPPSSPSPHFPTSLSIRSRFSIVSFATPESQVGEELDADTREWAMQDFYSLRKEVETTSERVEEIRNSAGLFQLDQELADLESKAADNALWDDRSKAQQVLVALTDVKDKIKMLTDFKTQVEEAETIVKLTEEMDSVDTGLLEEAASIIKDLNKALDQFELTQLLSGPYDKEGAVISISAGAGGTDAQDWADMLLRMYVRWGEKQRYKTRVVEKSAGEEAGIKSATIEIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSGVEVMPLLPDESMDVELPEEDLEISFSRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEERSQLANKIKALSRLKAKLLVIAEEQRASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVRTGYETSDIVSVMDGELEPFIKAYLKYKYSMALSTGGVN

Homology

[Close]

BLAST of Moc08g12310 vs. NCBI nr
Match: XP_022148293.1 (peptide chain release factor PrfB1, chloroplastic [Momordica charantia])

HSP 1 Score: 883.2 bits (2281), Expect = 9.3e-253
Identity = 458/458 (100.00%), Postives = 458/458 (100.00%), Query Frame = 0


Query: 1   MAVLLRPLWLCGSFSLVFPFPKFQSTRTSLSLEFPPPPSSPSPHFPTSLSIRSRFSIVSF 60
           MAVLLRPLWLCGSFSLVFPFPKFQSTRTSLSLEFPPPPSSPSPHFPTSLSIRSRFSIVSF
Sbjct: 1   MAVLLRPLWLCGSFSLVFPFPKFQSTRTSLSLEFPPPPSSPSPHFPTSLSIRSRFSIVSF 60

Query: 61  ATPESQVGEELDADTREWAMQDFYSLRKEVETTSERVEEIRNSAGLFQLDQELADLESKA 120
           ATPESQVGEELDADTREWAMQDFYSLRKEVETTSERVEEIRNSAGLFQLDQELADLESKA
Sbjct: 61  ATPESQVGEELDADTREWAMQDFYSLRKEVETTSERVEEIRNSAGLFQLDQELADLESKA 120

Query: 121 ADNALWDDRSKAQQVLVALTDVKDKIKMLTDFKTQVEEAETIVKLTEEMDSVDTGLLEEA 180
           ADNALWDDRSKAQQVLVALTDVKDKIKMLTDFKTQVEEAETIVKLTEEMDSVDTGLLEEA
Sbjct: 121 ADNALWDDRSKAQQVLVALTDVKDKIKMLTDFKTQVEEAETIVKLTEEMDSVDTGLLEEA 180

Query: 181 ASIIKDLNKALDQFELTQLLSGPYDKEGAVISISAGAGGTDAQDWADMLLRMYVRWGEKQ 240
           ASIIKDLNKALDQFELTQLLSGPYDKEGAVISISAGAGGTDAQDWADMLLRMYVRWGEKQ
Sbjct: 181 ASIIKDLNKALDQFELTQLLSGPYDKEGAVISISAGAGGTDAQDWADMLLRMYVRWGEKQ 240

Query: 241 RYKTRVVEKSAGEEAGIKSATIEIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSG 300
           RYKTRVVEKSAGEEAGIKSATIEIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSG
Sbjct: 241 RYKTRVVEKSAGEEAGIKSATIEIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSG 300

Query: 301 VEVMPLLPDESMDVELPEEDLEISFSRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEER 360
           VEVMPLLPDESMDVELPEEDLEISFSRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEER
Sbjct: 301 VEVMPLLPDESMDVELPEEDLEISFSRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEER 360

Query: 361 SQLANKIKALSRLKAKLLVIAEEQRASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVR 420
           SQLANKIKALSRLKAKLLVIAEEQRASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVR
Sbjct: 361 SQLANKIKALSRLKAKLLVIAEEQRASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVR 420

Query: 421 TGYETSDIVSVMDGELEPFIKAYLKYKYSMALSTGGVN 459
           TGYETSDIVSVMDGELEPFIKAYLKYKYSMALSTGGVN
Sbjct: 421 TGYETSDIVSVMDGELEPFIKAYLKYKYSMALSTGGVN 458

[Close]

BLAST of Moc08g12310 vs. NCBI nr
Match: XP_038887337.1 (peptide chain release factor PrfB1, chloroplastic-like isoform X1 [Benincasa hispida] >XP_038887338.1 peptide chain release factor PrfB1, chloroplastic-like isoform X2 [Benincasa hispida])

HSP 1 Score: 791.6 bits (2043), Expect = 3.7e-225
Identity = 420/455 (92.31%), Postives = 427/455 (93.85%), Query Frame = 0


Query: 1   MAVLLRPLWLCGSFSLVFPFPKFQSTRTSLSLEFPPPPSSPSPHFPTSLSIRSRFSIVSF 60
           M VL RPLWL G  S  FP PKFQST  S SLEF   P+SPSPH  T LSI SRFS+VSF
Sbjct: 8   MDVLHRPLWLGG--SPFFPLPKFQSTGASTSLEF--RPASPSPHLRTLLSIHSRFSLVSF 67

Query: 61  ATPESQVGEELDADTREWAMQDFYSLRKEVETTSERVEEIRNSAGLFQLDQELADLESKA 120
           ATPES VGE +DADTREWAMQDFY LRKEVETTSERVEEIRNSAGL QLDQELADLESKA
Sbjct: 68  ATPESPVGEAIDADTREWAMQDFYLLRKEVETTSERVEEIRNSAGLLQLDQELADLESKA 127

Query: 121 ADNALWDDRSKAQQVLVALTDVKDKIKMLTDFKTQVEEAETIVKLTEEMDSVDTGLLEEA 180
           AD++ WDDRS AQQVLVALTDVKDKI MLTDF+TQVEEAETIVKLTEEMDSVD GLLEEA
Sbjct: 128 ADSSFWDDRSMAQQVLVALTDVKDKINMLTDFRTQVEEAETIVKLTEEMDSVDAGLLEEA 187

Query: 181 ASIIKDLNKALDQFELTQLLSGPYDKEGAVISISAGAGGTDAQDWADMLLRMYVRWGEKQ 240
            SIIKDLNKALDQFEL+QLLSGPYDKEGAVISISAGAGGTDAQDWADMLLRMYVRWGEKQ
Sbjct: 188 TSIIKDLNKALDQFELSQLLSGPYDKEGAVISISAGAGGTDAQDWADMLLRMYVRWGEKQ 247

Query: 241 RYKTRVVEKSAGEEAGIKSATIEIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSG 300
           RYKTRVVEKSAGEEAGIKSATIEIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSG
Sbjct: 248 RYKTRVVEKSAGEEAGIKSATIEIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSG 307

Query: 301 VEVMPLLPDESMDVELPEEDLEISFSRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEER 360
           VEVMPLLPDESMDVELPEEDLEISFSRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEER
Sbjct: 308 VEVMPLLPDESMDVELPEEDLEISFSRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEER 367

Query: 361 SQLANKIKALSRLKAKLLVIAEEQRASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVR 420
           SQLANKIKALSRLKAKLLVIAEEQRASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVR
Sbjct: 368 SQLANKIKALSRLKAKLLVIAEEQRASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVR 427

Query: 421 TGYETSDIVSVMDGELEPFIKAYLKYKYSMALSTG 456
           TGYETSDIVSVMDGELE FIKAYLKYKYSMALSTG
Sbjct: 428 TGYETSDIVSVMDGELEHFIKAYLKYKYSMALSTG 458

[Close]

BLAST of Moc08g12310 vs. NCBI nr
Match: XP_022949524.1 (peptide chain release factor PrfB1, chloroplastic isoform X2 [Cucurbita moschata])

HSP 1 Score: 787.7 bits (2033), Expect = 5.3e-224
Identity = 414/458 (90.39%), Postives = 430/458 (93.89%), Query Frame = 0


Query: 1   MAVLLRPLWLCGSFSLVFPFPKFQSTRTSLSLEFPPPPSSPSPHFPTSLSIRSRFSIVSF 60
           M V+ RPLWLC   S VFP P+ Q+ RTS  L+F      P   F TSLSI SR S+V F
Sbjct: 1   MDVVQRPLWLC--TSPVFPLPRLQTARTSSPLQF------PLSRFRTSLSIHSRSSLVRF 60

Query: 61  ATPESQVGEELDADTREWAMQDFYSLRKEVETTSERVEEIRNSAGLFQLDQELADLESKA 120
           ATPESQVGEE+D+DT+EWAMQDFYSLRKEVET SERVEEIRNSAGL QLDQE+ADLESKA
Sbjct: 61  ATPESQVGEEVDSDTQEWAMQDFYSLRKEVETASERVEEIRNSAGLLQLDQEVADLESKA 120

Query: 121 ADNALWDDRSKAQQVLVALTDVKDKIKMLTDFKTQVEEAETIVKLTEEMDSVDTGLLEEA 180
           A+N+ WDDRSKAQQVL+ALTDVKDKIKMLTDFKTQVEEAETIVKLTEEMDSVDTGLLEEA
Sbjct: 121 AENSFWDDRSKAQQVLMALTDVKDKIKMLTDFKTQVEEAETIVKLTEEMDSVDTGLLEEA 180

Query: 181 ASIIKDLNKALDQFELTQLLSGPYDKEGAVISISAGAGGTDAQDWADMLLRMYVRWGEKQ 240
           ASIIKDLNKALDQFELTQLLSGPYDKEGAVI+ISAGAGGTDAQDWADMLLRMYVRWGEKQ
Sbjct: 181 ASIIKDLNKALDQFELTQLLSGPYDKEGAVITISAGAGGTDAQDWADMLLRMYVRWGEKQ 240

Query: 241 RYKTRVVEKSAGEEAGIKSATIEIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSG 300
           RYKTRVVEKSAGEEAGIKSATIEIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSG
Sbjct: 241 RYKTRVVEKSAGEEAGIKSATIEIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSG 300

Query: 301 VEVMPLLPDESMDVELPEEDLEISFSRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEER 360
           VEVMPLLPDESMDVE+PEEDLEISFSRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEER
Sbjct: 301 VEVMPLLPDESMDVEIPEEDLEISFSRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEER 360

Query: 361 SQLANKIKALSRLKAKLLVIAEEQRASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVR 420
           SQLANKIKALSRLKAKLLVIAEEQRASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVR
Sbjct: 361 SQLANKIKALSRLKAKLLVIAEEQRASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVR 420

Query: 421 TGYETSDIVSVMDGELEPFIKAYLKYKYSMALSTGGVN 459
           TG+ETSDIVSVMDGELEPFIKAYLKYKYSMAL+TG VN
Sbjct: 421 TGHETSDIVSVMDGELEPFIKAYLKYKYSMALATGEVN 450

[Close]

BLAST of Moc08g12310 vs. NCBI nr
Match: XP_022998734.1 (peptide chain release factor PrfB1, chloroplastic isoform X2 [Cucurbita maxima])

HSP 1 Score: 786.9 bits (2031), Expect = 9.1e-224
Identity = 414/458 (90.39%), Postives = 431/458 (94.10%), Query Frame = 0


Query: 1   MAVLLRPLWLCGSFSLVFPFPKFQSTRTSLSLEFPPPPSSPSPHFPTSLSIRSRFSIVSF 60
           M V+ RPLWLC   S VFP P+ Q+TRTS  L++      P   F TSLSI SR S+V F
Sbjct: 1   MDVVQRPLWLC--TSPVFPPPRLQTTRTSSPLQY------PLSRFRTSLSIHSRSSLVRF 60

Query: 61  ATPESQVGEELDADTREWAMQDFYSLRKEVETTSERVEEIRNSAGLFQLDQELADLESKA 120
           ATPESQVGEE+D+DT+EWAMQDFYSLRKEVET SERVEEIRNSAGL QLDQE+ADLESKA
Sbjct: 61  ATPESQVGEEVDSDTQEWAMQDFYSLRKEVETASERVEEIRNSAGLLQLDQEVADLESKA 120

Query: 121 ADNALWDDRSKAQQVLVALTDVKDKIKMLTDFKTQVEEAETIVKLTEEMDSVDTGLLEEA 180
           A+N+ WDDRSKAQQVL+ALTDVKDKIKMLTDFKTQVEEAETIVKLTEEMDSVDTGLLEEA
Sbjct: 121 AENSFWDDRSKAQQVLMALTDVKDKIKMLTDFKTQVEEAETIVKLTEEMDSVDTGLLEEA 180

Query: 181 ASIIKDLNKALDQFELTQLLSGPYDKEGAVISISAGAGGTDAQDWADMLLRMYVRWGEKQ 240
           ASIIKDLNKALDQFELTQLLSGPYDKEGAVI+ISAGAGGTDAQDWADMLLRMYVRWGEKQ
Sbjct: 181 ASIIKDLNKALDQFELTQLLSGPYDKEGAVITISAGAGGTDAQDWADMLLRMYVRWGEKQ 240

Query: 241 RYKTRVVEKSAGEEAGIKSATIEIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSG 300
           RYKTRVVEKSAGEEAGIKSATIEIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSG
Sbjct: 241 RYKTRVVEKSAGEEAGIKSATIEIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSG 300

Query: 301 VEVMPLLPDESMDVELPEEDLEISFSRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEER 360
           VEVMPLLPDESMDVE+PEEDLEISFSRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEER
Sbjct: 301 VEVMPLLPDESMDVEIPEEDLEISFSRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEER 360

Query: 361 SQLANKIKALSRLKAKLLVIAEEQRASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVR 420
           SQLANKIKALSRLKAKLLVIAEEQRASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVR
Sbjct: 361 SQLANKIKALSRLKAKLLVIAEEQRASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVR 420

Query: 421 TGYETSDIVSVMDGELEPFIKAYLKYKYSMALSTGGVN 459
           TG+ETSDIVSVMDGELEPFIKAYLKYKYSMAL+TG VN
Sbjct: 421 TGHETSDIVSVMDGELEPFIKAYLKYKYSMALATGEVN 450

[Close]

BLAST of Moc08g12310 vs. NCBI nr
Match: XP_023525877.1 (peptide chain release factor PrfB1, chloroplastic isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 784.6 bits (2025), Expect = 4.5e-223
Identity = 412/458 (89.96%), Postives = 430/458 (93.89%), Query Frame = 0


Query: 1   MAVLLRPLWLCGSFSLVFPFPKFQSTRTSLSLEFPPPPSSPSPHFPTSLSIRSRFSIVSF 60
           M V+ RPLWLC   S VFP P+ Q+TR+S  L+F      P   F +SLSI SR S+V F
Sbjct: 1   MDVVQRPLWLC--TSPVFPLPRLQTTRSSSELQF------PLSRFRSSLSIHSRSSLVPF 60

Query: 61  ATPESQVGEELDADTREWAMQDFYSLRKEVETTSERVEEIRNSAGLFQLDQELADLESKA 120
           ATPES VGEE+D+DT+EWAMQDFYSLRKEVET SERVEEIRNSAGL QLDQE+ADLESKA
Sbjct: 61  ATPESPVGEEVDSDTQEWAMQDFYSLRKEVETASERVEEIRNSAGLLQLDQEVADLESKA 120

Query: 121 ADNALWDDRSKAQQVLVALTDVKDKIKMLTDFKTQVEEAETIVKLTEEMDSVDTGLLEEA 180
           A+N+ WDDRSKAQQVL+ALTDVKDKIKMLTDFKTQVEEAETIVKLTEEMDSVDTGLLEEA
Sbjct: 121 AENSFWDDRSKAQQVLMALTDVKDKIKMLTDFKTQVEEAETIVKLTEEMDSVDTGLLEEA 180

Query: 181 ASIIKDLNKALDQFELTQLLSGPYDKEGAVISISAGAGGTDAQDWADMLLRMYVRWGEKQ 240
           ASIIKDLNKALDQFELTQLLSGPYDKEGAVI+ISAGAGGTDAQDWADMLLRMYVRWGEKQ
Sbjct: 181 ASIIKDLNKALDQFELTQLLSGPYDKEGAVITISAGAGGTDAQDWADMLLRMYVRWGEKQ 240

Query: 241 RYKTRVVEKSAGEEAGIKSATIEIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSG 300
           RYKTRVVEKSAGEEAGIKSATIEIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSG
Sbjct: 241 RYKTRVVEKSAGEEAGIKSATIEIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSG 300

Query: 301 VEVMPLLPDESMDVELPEEDLEISFSRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEER 360
           VEVMPLLPDESMDVE+PEEDLEISFSRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEER
Sbjct: 301 VEVMPLLPDESMDVEIPEEDLEISFSRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEER 360

Query: 361 SQLANKIKALSRLKAKLLVIAEEQRASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVR 420
           SQLANKIKALSRLKAKLLVIAEEQRASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVR
Sbjct: 361 SQLANKIKALSRLKAKLLVIAEEQRASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVR 420

Query: 421 TGYETSDIVSVMDGELEPFIKAYLKYKYSMALSTGGVN 459
           TG+ETSDIVSVMDGELEPFIKAYLKYKYSMAL+TG VN
Sbjct: 421 TGHETSDIVSVMDGELEPFIKAYLKYKYSMALATGEVN 450

[Close]

BLAST of Moc08g12310 vs. ExPASy Swiss-Prot
Match: Q9LVY0 (Peptide chain release factor PrfB1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PRFB1 PE=2 SV=1)

HSP 1 Score: 625.9 bits (1613), Expect = 3.5e-178
Identity = 318/417 (76.26%), Postives = 369/417 (88.49%), Query Frame = 0


Query: 43  PHFPTSLSIRSRF-SIVSFATPESQVGEELDADTREWAMQDFYSLRKEVETTSERVEEIR 102
           P+F +S    SRF S + FA  ES +    + +T EW MQDFY+LRK+VE  S RVEEIR
Sbjct: 44  PNFSSS----SRFNSPIVFAAQESNLSVSNENETSEWLMQDFYTLRKDVEIASARVEEIR 103

Query: 103 NSAGLFQLDQELADLESKAADNALWDDRSKAQQVLVALTDVKDKIKMLTDFKTQVEEAET 162
            SA L QL+QE+ +LESKA D + WDDR+KAQ+ L +L D+KD++++L++FKT VE+AET
Sbjct: 104 ASANLQQLEQEITNLESKATDTSFWDDRTKAQETLSSLNDLKDRMRLLSEFKTMVEDAET 163

Query: 163 IVKLTEEMDSVDTGLLEEAASIIKDLNKALDQFELTQLLSGPYDKEGAVISISAGAGGTD 222
           IVKLTEEMDS D  LLEEA  IIK+LNK+LD+FELTQLLSGPYDKEGAV+ I+AGAGGTD
Sbjct: 164 IVKLTEEMDSTDVSLLEEAMGIIKELNKSLDKFELTQLLSGPYDKEGAVVYITAGAGGTD 223

Query: 223 AQDWADMLLRMYVRWGEKQRYKTRVVEKSAGEEAGIKSATIEIEGRYAYGYISGEKGTHR 282
           AQDWADMLLRMY+RWGEKQRYKT+VVE S GEEAGIKSAT+EIEGRYAYGYISGEKGTHR
Sbjct: 224 AQDWADMLLRMYMRWGEKQRYKTKVVEMSNGEEAGIKSATLEIEGRYAYGYISGEKGTHR 283

Query: 283 IVRQSPFNSKGLRQTSFSGVEVMPLLPDESMDVELPEEDLEISFSRAGGKGGQNVNKVET 342
           IVRQSPFNSKGLRQTSFSGVEVMPLLP+E++ +E+PEEDL+ISF+RAGGKGGQNVNKVET
Sbjct: 284 IVRQSPFNSKGLRQTSFSGVEVMPLLPEEAVGIEIPEEDLDISFTRAGGKGGQNVNKVET 343

Query: 343 AVRITHIPTGVTVRCTEERSQLANKIKALSRLKAKLLVIAEEQRASEIKQIRGDAVKAEW 402
           AVRITHIPTGV VRCTEERSQLANK +AL RLKAKL+VIAEEQRA+EIK+IRGDAVKAEW
Sbjct: 344 AVRITHIPTGVAVRCTEERSQLANKTRALIRLKAKLMVIAEEQRATEIKEIRGDAVKAEW 403

Query: 403 GQQIRNYVFHPYKLVKDVRTGYETSDIVSVMDGELEPFIKAYLKYKYSMALSTGGVN 459
           GQQIRNYVFHPYKLVKDVRTG+ETSDI SVMDG+L+PFIKAYLK+KY++A+++   N
Sbjct: 404 GQQIRNYVFHPYKLVKDVRTGHETSDITSVMDGDLDPFIKAYLKHKYTLAMASAVTN 456

[Close]

BLAST of Moc08g12310 vs. ExPASy Swiss-Prot
Match: P74476 (Peptide chain release factor 2 OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 GN=prfB PE=3 SV=2)

HSP 1 Score: 417.2 bits (1071), Expect = 2.5e-115
Identity = 212/361 (58.73%), Postives = 276/361 (76.45%), Query Frame = 0


Query: 86  LRKEVETTSERVEEIRNSAGLFQLDQELADLESKAADNALWDDRSKAQQVLVALTDVKDK 145
           L++ +E  S R+ + ++   L  L  ++ DLE  AA    WDD  +AQQ+L  L + K +
Sbjct: 8   LKRNLELISSRLGQTQDYLDLPGLKAKVQDLEQCAAQPDFWDDTDQAQQILQTLNETKSQ 67

Query: 146 IKMLTDFKTQVEEAETIVKLTEEMDSVDTGLLEEAASIIKDLNKALDQFELTQLLSGPYD 205
           ++    ++ Q ++++ IV+L E  D  D  LL EA + ++ L K LD++EL QLLSGPYD
Sbjct: 68  LEQWGIWQQQWQDSQAIVELLELED--DQALLTEAETTLEQLQKELDRWELQQLLSGPYD 127

Query: 206 KEGAVISISAGAGGTDAQDWADMLLRMYVRWGEKQRYKTRVVEKSAGEEAGIKSATIEIE 265
            +GA ++I+AGAGGTDAQDWA+MLLRMY RW EKQ YK  + E S G+EAG+KS T+EIE
Sbjct: 128 AKGATLTINAGAGGTDAQDWAEMLLRMYTRWSEKQGYKVHLAEISEGDEAGLKSVTLEIE 187

Query: 266 GRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSGVEVMPLLPDESMDVELPEEDLEISF 325
           GRYAYGY+  EKGTHR+VR SPFN+ G RQTSF+GVEVMPLL +E++ +++P++DL+IS 
Sbjct: 188 GRYAYGYLKSEKGTHRLVRISPFNANGKRQTSFAGVEVMPLLGEEAISLDIPDKDLDIST 247

Query: 326 SRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEERSQLANKIKALSRLKAKLLVIAEEQR 385
           SRAGGKGGQNVNKVETAVRI H+PTG+ VRCT+ERSQL NK KAL+ LKAKLL++ EEQR
Sbjct: 248 SRAGGKGGQNVNKVETAVRIVHLPTGLAVRCTQERSQLQNKEKALAILKAKLLIVLEEQR 307

Query: 386 ASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVRTGYETSDIVSVMDGELEPFIKAYLK 445
           A  I +IRGD V+A WG QIRNYVFHPY+LVKD+RT  ET+D+  VMDGEL  FI+AYL+
Sbjct: 308 AQAIAEIRGDMVEAAWGTQIRNYVFHPYQLVKDLRTNVETTDVGGVMDGELSDFIEAYLR 366

Query: 446 Y 447
           +
Sbjct: 368 H 366

[Close]

BLAST of Moc08g12310 vs. ExPASy Swiss-Prot
Match: B7K0A6 (Peptide chain release factor 2 OS=Rippkaea orientalis (strain PCC 8801) OX=41431 GN=prfB PE=3 SV=1)

HSP 1 Score: 410.2 bits (1053), Expect = 3.0e-113
Identity = 208/360 (57.78%), Postives = 275/360 (76.39%), Query Frame = 0


Query: 86  LRKEVETTSERVEEIRNSAGLFQLDQELADLESKAADNALWDDRSKAQQVLVALTDVKDK 145
           L++E++  + R+ + ++   L  L+  + DLE  AA    WD++  AQ+ L  L D+K  
Sbjct: 6   LKREIQQIAARLGKTQDYLDLPALNANIQDLEQIAAQPDFWDNQETAQKTLQQLNDLKSS 65

Query: 146 IKMLTDFKTQVEEAETIVKLTEEMDSVDTGLLEEAASIIKDLNKALDQFELTQLLSGPYD 205
           ++    +  Q+E+ + I +L E  +  D  L EEA + +  LN  LD++EL +LLSG YD
Sbjct: 66  VEEYHQWMGQLEDLKAIAELLELEE--DATLNEEAEANLTQLNHELDRWELQRLLSGIYD 125

Query: 206 KEGAVISISAGAGGTDAQDWADMLLRMYVRWGEKQRYKTRVVEKSAGEEAGIKSATIEIE 265
            +GAV++I+AGAGGTDAQDWA+MLLRMY RWGE+Q YK  + E S G+EAGIKSAT+EIE
Sbjct: 126 SKGAVLTINAGAGGTDAQDWAEMLLRMYTRWGEQQGYKVHLTEISEGDEAGIKSATLEIE 185

Query: 266 GRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSGVEVMPLLPDESMDVELPEEDLEISF 325
           GRYAYGY+ GEKGTHR+VR SPFN+ G RQTSF+G+EVMP L +E + VE+PE+DLEI+ 
Sbjct: 186 GRYAYGYLKGEKGTHRLVRISPFNANGKRQTSFAGIEVMPALEEEDLKVEIPEKDLEITT 245

Query: 326 SRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEERSQLANKIKALSRLKAKLLVIAEEQR 385
           +R+GGKGGQNVNKVETAVR+ H+PTG+ VRCT+ERSQL NK KAL+ LKAKLL+IA+EQR
Sbjct: 246 TRSGGKGGQNVNKVETAVRVVHLPTGIAVRCTQERSQLQNKEKALALLKAKLLIIAQEQR 305

Query: 386 ASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVRTGYETSDIVSVMDGELEPFIKAYLK 445
           A  I +IRGD V+A WG QIRNYVFHPY++VKDVRT  ET+D+  VMDG+L+ FI++YL+
Sbjct: 306 AQAIAEIRGDMVEAAWGNQIRNYVFHPYQMVKDVRTNTETTDVNGVMDGKLDLFIESYLR 363

[Close]

BLAST of Moc08g12310 vs. ExPASy Swiss-Prot
Match: Q7U3W6 (Peptide chain release factor 2 OS=Synechococcus sp. (strain WH8102) OX=84588 GN=prfB PE=3 SV=1)

HSP 1 Score: 391.0 bits (1003), Expect = 1.9e-107
Identity = 204/364 (56.04%), Postives = 266/364 (73.08%), Query Frame = 0


Query: 82  DFYSLRKEVETTSERVEEIRNSAGLFQLDQELADLESKAADNALWDDRSKAQQVLVALTD 141
           D    ++++   ++R+   ++   +  L     DLE  AA    WDD+  AQ+ +  L +
Sbjct: 2   DLTDFKRDLSELTDRLGHAQDCLDVPALKARQQDLEQLAAQPDFWDDQQAAQKQMRRLDE 61

Query: 142 VKDKIKMLTDFKTQVEEAETIVKLTEEMDSVDTGLLEEAASIIKDLNKALDQFELTQLLS 201
           VK +++ L D+   V++A+  ++L E     D  +L EA   +  L + LD++EL +LLS
Sbjct: 62  VKAQLQQLADWGGAVDDAKATLELYEL--EPDEEMLTEAQEGLNQLRQGLDRWELERLLS 121

Query: 202 GPYDKEGAVISISAGAGGTDAQDWADMLLRMYVRWGEKQRYKTRVVEKSAGEEAGIKSAT 261
           G YDKEGAV++I+AGAGGTDAQDWA MLLRMY RW E    K  V E S GEEAGIKS T
Sbjct: 122 GDYDKEGAVLTINAGAGGTDAQDWAQMLLRMYTRWAEDHGMKVTVDELSEGEEAGIKSCT 181

Query: 262 IEIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSGVEVMPLLPDESMDVELPEEDL 321
           IE+EGRYAYGY+  EKGTHR+VR SPFN+   RQTSF+G+EVMP + DE +D+++PE+DL
Sbjct: 182 IEVEGRYAYGYLRNEKGTHRLVRISPFNANDKRQTSFAGIEVMPKI-DEEVDIDIPEKDL 241

Query: 322 EISFSRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEERSQLANKIKALSRLKAKLLVIA 381
           E++ SR+GG GGQNVNKVETAVRI HIPTG+ VRCT+ERSQL NK KA++ LKAKLLVIA
Sbjct: 242 EVTTSRSGGAGGQNVNKVETAVRILHIPTGLAVRCTQERSQLQNKEKAMALLKAKLLVIA 301

Query: 382 EEQRASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVRTGYETSDIVSVMDGELEPFIK 441
           +EQRA+EI  IRGD V+A WG QIRNYVFHPY++VKD+RT  ET+D+ +VMDG L+PFI 
Sbjct: 302 QEQRAAEIADIRGDIVEAAWGNQIRNYVFHPYQMVKDLRTSEETNDVQAVMDGALDPFID 361

Query: 442 AYLK 446
           A L+
Sbjct: 362 ASLR 362

[Close]

BLAST of Moc08g12310 vs. ExPASy Swiss-Prot
Match: O67695 (Peptide chain release factor 2 OS=Aquifex aeolicus (strain VF5) OX=224324 GN=prfB PE=3 SV=1)

HSP 1 Score: 325.5 bits (833), Expect = 9.8e-88
Identity = 166/362 (45.86%), Postives = 245/362 (67.68%), Query Frame = 0


Query: 86  LRKEVETTSERVEEIRNSAGLFQLDQELADLESKAADNALWDDRSKAQQVLVALTDVKDK 145
           L+ +VE   +R+E+++      +L+ EL +L+ K ++   W+D+ KA+QV+     V++ 
Sbjct: 6   LKGKVEELRKRLEDVKKILSPEKLESELKELDQKMSEPNFWEDQEKAKQVIQRRKWVEET 65

Query: 146 IKMLTDFKTQVEEAETIVKLTEEMDSVDTGLLEEAASIIKDLNKALDQFELTQLLSGPYD 205
           +  L + +  V++ E +V++T E D+    +++E    IK++ + L + EL   LSG  D
Sbjct: 66  LNKLKNLEKSVKDLEELVEITSEEDTETWAMMDEE---IKEVERTLRELELKTYLSGEMD 125

Query: 206 KEGAVISISAGAGGTDAQDWADMLLRMYVRWGEKQRYKTRVVEKSAGEEAGIKSATIEIE 265
            + A ++I AGAGGT+A DWADML RMY RW EK+ Y+  +++ +  + AGIKS T+ ++
Sbjct: 126 AKNAYLTIQAGAGGTEACDWADMLFRMYKRWAEKKGYEVELIDITPDDVAGIKSVTVLVK 185

Query: 266 GRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSGVEVMPLLPDESMDVELPEEDLEISF 325
           G YAYGY+ GE+G HR+VR SPF++   R TSF+ V VMP + DE + +E+  EDL+I  
Sbjct: 186 GPYAYGYLKGEQGVHRLVRISPFDANARRHTSFAAVSVMPQI-DEDIKIEIKPEDLKIET 245

Query: 326 SRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEERSQLANKIKALSRLKAKLLVIAEEQR 385
            RA G GGQ VNK +TAVRITHIPTG+TV C +ERSQ  NK KAL  LKAKL  +  ++ 
Sbjct: 246 FRASGAGGQYVNKTDTAVRITHIPTGITVSCQQERSQYQNKRKALELLKAKLYQLEMKKL 305

Query: 386 ASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVRTGYETSDIVSVMDGELEPFIKAYLK 445
             + KQ  G+     WG QIR+YVFHPYKL+KD+RTGYET ++ +VMDGE++ FI++YLK
Sbjct: 306 EEKKKQYEGEKTDIGWGHQIRSYVFHPYKLIKDLRTGYETGNVEAVMDGEIDEFIESYLK 363

Query: 446 YK 448
           +K
Sbjct: 366 WK 363

[Close]

BLAST of Moc08g12310 vs. ExPASy TrEMBL
Match: A0A6J1D3Q2 (peptide chain release factor PrfB1, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111016981 PE=3 SV=1)

HSP 1 Score: 883.2 bits (2281), Expect = 4.5e-253
Identity = 458/458 (100.00%), Postives = 458/458 (100.00%), Query Frame = 0


Query: 1   MAVLLRPLWLCGSFSLVFPFPKFQSTRTSLSLEFPPPPSSPSPHFPTSLSIRSRFSIVSF 60
           MAVLLRPLWLCGSFSLVFPFPKFQSTRTSLSLEFPPPPSSPSPHFPTSLSIRSRFSIVSF
Sbjct: 1   MAVLLRPLWLCGSFSLVFPFPKFQSTRTSLSLEFPPPPSSPSPHFPTSLSIRSRFSIVSF 60

Query: 61  ATPESQVGEELDADTREWAMQDFYSLRKEVETTSERVEEIRNSAGLFQLDQELADLESKA 120
           ATPESQVGEELDADTREWAMQDFYSLRKEVETTSERVEEIRNSAGLFQLDQELADLESKA
Sbjct: 61  ATPESQVGEELDADTREWAMQDFYSLRKEVETTSERVEEIRNSAGLFQLDQELADLESKA 120

Query: 121 ADNALWDDRSKAQQVLVALTDVKDKIKMLTDFKTQVEEAETIVKLTEEMDSVDTGLLEEA 180
           ADNALWDDRSKAQQVLVALTDVKDKIKMLTDFKTQVEEAETIVKLTEEMDSVDTGLLEEA
Sbjct: 121 ADNALWDDRSKAQQVLVALTDVKDKIKMLTDFKTQVEEAETIVKLTEEMDSVDTGLLEEA 180

Query: 181 ASIIKDLNKALDQFELTQLLSGPYDKEGAVISISAGAGGTDAQDWADMLLRMYVRWGEKQ 240
           ASIIKDLNKALDQFELTQLLSGPYDKEGAVISISAGAGGTDAQDWADMLLRMYVRWGEKQ
Sbjct: 181 ASIIKDLNKALDQFELTQLLSGPYDKEGAVISISAGAGGTDAQDWADMLLRMYVRWGEKQ 240

Query: 241 RYKTRVVEKSAGEEAGIKSATIEIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSG 300
           RYKTRVVEKSAGEEAGIKSATIEIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSG
Sbjct: 241 RYKTRVVEKSAGEEAGIKSATIEIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSG 300

Query: 301 VEVMPLLPDESMDVELPEEDLEISFSRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEER 360
           VEVMPLLPDESMDVELPEEDLEISFSRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEER
Sbjct: 301 VEVMPLLPDESMDVELPEEDLEISFSRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEER 360

Query: 361 SQLANKIKALSRLKAKLLVIAEEQRASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVR 420
           SQLANKIKALSRLKAKLLVIAEEQRASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVR
Sbjct: 361 SQLANKIKALSRLKAKLLVIAEEQRASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVR 420

Query: 421 TGYETSDIVSVMDGELEPFIKAYLKYKYSMALSTGGVN 459
           TGYETSDIVSVMDGELEPFIKAYLKYKYSMALSTGGVN
Sbjct: 421 TGYETSDIVSVMDGELEPFIKAYLKYKYSMALSTGGVN 458

[Close]

BLAST of Moc08g12310 vs. ExPASy TrEMBL
Match: A0A6J1GD28 (peptide chain release factor PrfB1, chloroplastic isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111452850 PE=3 SV=1)

HSP 1 Score: 787.7 bits (2033), Expect = 2.6e-224
Identity = 414/458 (90.39%), Postives = 430/458 (93.89%), Query Frame = 0


Query: 1   MAVLLRPLWLCGSFSLVFPFPKFQSTRTSLSLEFPPPPSSPSPHFPTSLSIRSRFSIVSF 60
           M V+ RPLWLC   S VFP P+ Q+ RTS  L+F      P   F TSLSI SR S+V F
Sbjct: 1   MDVVQRPLWLC--TSPVFPLPRLQTARTSSPLQF------PLSRFRTSLSIHSRSSLVRF 60

Query: 61  ATPESQVGEELDADTREWAMQDFYSLRKEVETTSERVEEIRNSAGLFQLDQELADLESKA 120
           ATPESQVGEE+D+DT+EWAMQDFYSLRKEVET SERVEEIRNSAGL QLDQE+ADLESKA
Sbjct: 61  ATPESQVGEEVDSDTQEWAMQDFYSLRKEVETASERVEEIRNSAGLLQLDQEVADLESKA 120

Query: 121 ADNALWDDRSKAQQVLVALTDVKDKIKMLTDFKTQVEEAETIVKLTEEMDSVDTGLLEEA 180
           A+N+ WDDRSKAQQVL+ALTDVKDKIKMLTDFKTQVEEAETIVKLTEEMDSVDTGLLEEA
Sbjct: 121 AENSFWDDRSKAQQVLMALTDVKDKIKMLTDFKTQVEEAETIVKLTEEMDSVDTGLLEEA 180

Query: 181 ASIIKDLNKALDQFELTQLLSGPYDKEGAVISISAGAGGTDAQDWADMLLRMYVRWGEKQ 240
           ASIIKDLNKALDQFELTQLLSGPYDKEGAVI+ISAGAGGTDAQDWADMLLRMYVRWGEKQ
Sbjct: 181 ASIIKDLNKALDQFELTQLLSGPYDKEGAVITISAGAGGTDAQDWADMLLRMYVRWGEKQ 240

Query: 241 RYKTRVVEKSAGEEAGIKSATIEIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSG 300
           RYKTRVVEKSAGEEAGIKSATIEIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSG
Sbjct: 241 RYKTRVVEKSAGEEAGIKSATIEIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSG 300

Query: 301 VEVMPLLPDESMDVELPEEDLEISFSRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEER 360
           VEVMPLLPDESMDVE+PEEDLEISFSRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEER
Sbjct: 301 VEVMPLLPDESMDVEIPEEDLEISFSRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEER 360

Query: 361 SQLANKIKALSRLKAKLLVIAEEQRASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVR 420
           SQLANKIKALSRLKAKLLVIAEEQRASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVR
Sbjct: 361 SQLANKIKALSRLKAKLLVIAEEQRASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVR 420

Query: 421 TGYETSDIVSVMDGELEPFIKAYLKYKYSMALSTGGVN 459
           TG+ETSDIVSVMDGELEPFIKAYLKYKYSMAL+TG VN
Sbjct: 421 TGHETSDIVSVMDGELEPFIKAYLKYKYSMALATGEVN 450

[Close]

BLAST of Moc08g12310 vs. ExPASy TrEMBL
Match: A0A6J1KF53 (peptide chain release factor PrfB1, chloroplastic isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111493309 PE=3 SV=1)

HSP 1 Score: 786.9 bits (2031), Expect = 4.4e-224
Identity = 414/458 (90.39%), Postives = 431/458 (94.10%), Query Frame = 0


Query: 1   MAVLLRPLWLCGSFSLVFPFPKFQSTRTSLSLEFPPPPSSPSPHFPTSLSIRSRFSIVSF 60
           M V+ RPLWLC   S VFP P+ Q+TRTS  L++      P   F TSLSI SR S+V F
Sbjct: 1   MDVVQRPLWLC--TSPVFPPPRLQTTRTSSPLQY------PLSRFRTSLSIHSRSSLVRF 60

Query: 61  ATPESQVGEELDADTREWAMQDFYSLRKEVETTSERVEEIRNSAGLFQLDQELADLESKA 120
           ATPESQVGEE+D+DT+EWAMQDFYSLRKEVET SERVEEIRNSAGL QLDQE+ADLESKA
Sbjct: 61  ATPESQVGEEVDSDTQEWAMQDFYSLRKEVETASERVEEIRNSAGLLQLDQEVADLESKA 120

Query: 121 ADNALWDDRSKAQQVLVALTDVKDKIKMLTDFKTQVEEAETIVKLTEEMDSVDTGLLEEA 180
           A+N+ WDDRSKAQQVL+ALTDVKDKIKMLTDFKTQVEEAETIVKLTEEMDSVDTGLLEEA
Sbjct: 121 AENSFWDDRSKAQQVLMALTDVKDKIKMLTDFKTQVEEAETIVKLTEEMDSVDTGLLEEA 180

Query: 181 ASIIKDLNKALDQFELTQLLSGPYDKEGAVISISAGAGGTDAQDWADMLLRMYVRWGEKQ 240
           ASIIKDLNKALDQFELTQLLSGPYDKEGAVI+ISAGAGGTDAQDWADMLLRMYVRWGEKQ
Sbjct: 181 ASIIKDLNKALDQFELTQLLSGPYDKEGAVITISAGAGGTDAQDWADMLLRMYVRWGEKQ 240

Query: 241 RYKTRVVEKSAGEEAGIKSATIEIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSG 300
           RYKTRVVEKSAGEEAGIKSATIEIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSG
Sbjct: 241 RYKTRVVEKSAGEEAGIKSATIEIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSG 300

Query: 301 VEVMPLLPDESMDVELPEEDLEISFSRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEER 360
           VEVMPLLPDESMDVE+PEEDLEISFSRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEER
Sbjct: 301 VEVMPLLPDESMDVEIPEEDLEISFSRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEER 360

Query: 361 SQLANKIKALSRLKAKLLVIAEEQRASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVR 420
           SQLANKIKALSRLKAKLLVIAEEQRASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVR
Sbjct: 361 SQLANKIKALSRLKAKLLVIAEEQRASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVR 420

Query: 421 TGYETSDIVSVMDGELEPFIKAYLKYKYSMALSTGGVN 459
           TG+ETSDIVSVMDGELEPFIKAYLKYKYSMAL+TG VN
Sbjct: 421 TGHETSDIVSVMDGELEPFIKAYLKYKYSMALATGEVN 450

[Close]

BLAST of Moc08g12310 vs. ExPASy TrEMBL
Match: A0A0A0LY01 (RF_PROK_I domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G652260 PE=3 SV=1)

HSP 1 Score: 785.4 bits (2027), Expect = 1.3e-223
Identity = 412/455 (90.55%), Postives = 428/455 (94.07%), Query Frame = 0


Query: 1   MAVLLRPLWLCGSFSLVFPFPKFQSTRTSLSLEFPPPPSSPSPHFPTSLSIRSRFSIVSF 60
           M VL R LWL G+  +  P PKFQST  S+SL+F   P+SPSPHF TSLS  SRFS+V F
Sbjct: 1   MDVLHRTLWL-GTSPIFLPLPKFQSTGASISLQF--RPTSPSPHFRTSLSTHSRFSLVPF 60

Query: 61  ATPESQVGEELDADTREWAMQDFYSLRKEVETTSERVEEIRNSAGLFQLDQELADLESKA 120
           AT ES VGE +D DTREWAMQDFY+LRKEVETTSERVEEIRNSAGL QLDQELADLESKA
Sbjct: 61  ATHESPVGEAIDTDTREWAMQDFYTLRKEVETTSERVEEIRNSAGLLQLDQELADLESKA 120

Query: 121 ADNALWDDRSKAQQVLVALTDVKDKIKMLTDFKTQVEEAETIVKLTEEMDSVDTGLLEEA 180
           ADN+ WDDRSKAQ+VL+A+TDVKDKIKMLTDFKTQVEEAETIVKLTEEMDSVD GLLEEA
Sbjct: 121 ADNSFWDDRSKAQKVLMAMTDVKDKIKMLTDFKTQVEEAETIVKLTEEMDSVDVGLLEEA 180

Query: 181 ASIIKDLNKALDQFELTQLLSGPYDKEGAVISISAGAGGTDAQDWADMLLRMYVRWGEKQ 240
             IIKDLNKALDQFEL++LLSGPYDKEGAVISISAGAGGTDAQDWADMLLRMYVRWGEKQ
Sbjct: 181 TKIIKDLNKALDQFELSELLSGPYDKEGAVISISAGAGGTDAQDWADMLLRMYVRWGEKQ 240

Query: 241 RYKTRVVEKSAGEEAGIKSATIEIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSG 300
           RYKTRVVEKSAGEEAGIKSATIEIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSG
Sbjct: 241 RYKTRVVEKSAGEEAGIKSATIEIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSG 300

Query: 301 VEVMPLLPDESMDVELPEEDLEISFSRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEER 360
           VEVMPLLPDESM+VELPEEDLEISFSRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEER
Sbjct: 301 VEVMPLLPDESMNVELPEEDLEISFSRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEER 360

Query: 361 SQLANKIKALSRLKAKLLVIAEEQRASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVR 420
           SQLANKIKALSRLKAKLLVIAEEQRASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVR
Sbjct: 361 SQLANKIKALSRLKAKLLVIAEEQRASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVR 420

Query: 421 TGYETSDIVSVMDGELEPFIKAYLKYKYSMALSTG 456
           TGYETSDIVSVMDGELEPFIKAYLKYKYS+ALSTG
Sbjct: 421 TGYETSDIVSVMDGELEPFIKAYLKYKYSIALSTG 452

[Close]

BLAST of Moc08g12310 vs. ExPASy TrEMBL
Match: Q6ZY50 (Putative translation releasing factor 2 OS=Cucumis sativus OX=3659 GN=rf2 PE=2 SV=1)

HSP 1 Score: 783.1 bits (2021), Expect = 6.3e-223
Identity = 411/455 (90.33%), Postives = 427/455 (93.85%), Query Frame = 0


Query: 1   MAVLLRPLWLCGSFSLVFPFPKFQSTRTSLSLEFPPPPSSPSPHFPTSLSIRSRFSIVSF 60
           M VL R LWL G+  +  P PKFQST  S+SL+F   P+SPSPHF TSLS  SRFS+V F
Sbjct: 1   MDVLHRTLWL-GTSPIFLPLPKFQSTGASISLQF--RPTSPSPHFRTSLSTHSRFSLVPF 60

Query: 61  ATPESQVGEELDADTREWAMQDFYSLRKEVETTSERVEEIRNSAGLFQLDQELADLESKA 120
           AT ES VGE +D DTREWA QDFY+LRKEVETTSERVEEIRNSAGL QLDQELADLESKA
Sbjct: 61  ATHESPVGEAIDTDTREWATQDFYTLRKEVETTSERVEEIRNSAGLLQLDQELADLESKA 120

Query: 121 ADNALWDDRSKAQQVLVALTDVKDKIKMLTDFKTQVEEAETIVKLTEEMDSVDTGLLEEA 180
           ADN+ WDDRSKAQ+VL+A+TDVKDKIKMLTDFKTQVEEAETIVKLTEEMDSVD GLLEEA
Sbjct: 121 ADNSFWDDRSKAQKVLMAMTDVKDKIKMLTDFKTQVEEAETIVKLTEEMDSVDVGLLEEA 180

Query: 181 ASIIKDLNKALDQFELTQLLSGPYDKEGAVISISAGAGGTDAQDWADMLLRMYVRWGEKQ 240
             IIKDLNKALDQFEL++LLSGPYDKEGAVISISAGAGGTDAQDWADMLLRMYVRWGEKQ
Sbjct: 181 TKIIKDLNKALDQFELSELLSGPYDKEGAVISISAGAGGTDAQDWADMLLRMYVRWGEKQ 240

Query: 241 RYKTRVVEKSAGEEAGIKSATIEIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSG 300
           RYKTRVVEKSAGEEAGIKSATIEIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSG
Sbjct: 241 RYKTRVVEKSAGEEAGIKSATIEIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSG 300

Query: 301 VEVMPLLPDESMDVELPEEDLEISFSRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEER 360
           VEVMPLLPDESM+VELPEEDLEISFSRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEER
Sbjct: 301 VEVMPLLPDESMNVELPEEDLEISFSRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEER 360

Query: 361 SQLANKIKALSRLKAKLLVIAEEQRASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVR 420
           SQLANKIKALSRLKAKLLVIAEEQRASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVR
Sbjct: 361 SQLANKIKALSRLKAKLLVIAEEQRASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVR 420

Query: 421 TGYETSDIVSVMDGELEPFIKAYLKYKYSMALSTG 456
           TGYETSDIVSVMDGELEPFIKAYLKYKYS+ALSTG
Sbjct: 421 TGYETSDIVSVMDGELEPFIKAYLKYKYSIALSTG 452

[Close]

BLAST of Moc08g12310 vs. TAIR 10
Match: AT5G36170.2 (high chlorophyll fluorescent 109 )

HSP 1 Score: 627.9 bits (1618), Expect = 6.6e-180
Identity = 317/416 (76.20%), Postives = 368/416 (88.46%), Query Frame = 0


Query: 43  PHFPTSLSIRSRFSIVSFATPESQVGEELDADTREWAMQDFYSLRKEVETTSERVEEIRN 102
           P+F +S    SRF+   FA  ES +    + +T EW MQDFY+LRK+VE  S RVEEIR 
Sbjct: 44  PNFSSS----SRFNSPIFAAQESNLSVSNENETSEWLMQDFYTLRKDVEIASARVEEIRA 103

Query: 103 SAGLFQLDQELADLESKAADNALWDDRSKAQQVLVALTDVKDKIKMLTDFKTQVEEAETI 162
           SA L QL+QE+ +LESKA D + WDDR+KAQ+ L +L D+KD++++L++FKT VE+AETI
Sbjct: 104 SANLQQLEQEITNLESKATDTSFWDDRTKAQETLSSLNDLKDRMRLLSEFKTMVEDAETI 163

Query: 163 VKLTEEMDSVDTGLLEEAASIIKDLNKALDQFELTQLLSGPYDKEGAVISISAGAGGTDA 222
           VKLTEEMDS D  LLEEA  IIK+LNK+LD+FELTQLLSGPYDKEGAV+ I+AGAGGTDA
Sbjct: 164 VKLTEEMDSTDVSLLEEAMGIIKELNKSLDKFELTQLLSGPYDKEGAVVYITAGAGGTDA 223

Query: 223 QDWADMLLRMYVRWGEKQRYKTRVVEKSAGEEAGIKSATIEIEGRYAYGYISGEKGTHRI 282
           QDWADMLLRMY+RWGEKQRYKT+VVE S GEEAGIKSAT+EIEGRYAYGYISGEKGTHRI
Sbjct: 224 QDWADMLLRMYMRWGEKQRYKTKVVEMSNGEEAGIKSATLEIEGRYAYGYISGEKGTHRI 283

Query: 283 VRQSPFNSKGLRQTSFSGVEVMPLLPDESMDVELPEEDLEISFSRAGGKGGQNVNKVETA 342
           VRQSPFNSKGLRQTSFSGVEVMPLLP+E++ +E+PEEDL+ISF+RAGGKGGQNVNKVETA
Sbjct: 284 VRQSPFNSKGLRQTSFSGVEVMPLLPEEAVGIEIPEEDLDISFTRAGGKGGQNVNKVETA 343

Query: 343 VRITHIPTGVTVRCTEERSQLANKIKALSRLKAKLLVIAEEQRASEIKQIRGDAVKAEWG 402
           VRITHIPTGV VRCTEERSQLANK +AL RLKAKL+VIAEEQRA+EIK+IRGDAVKAEWG
Sbjct: 344 VRITHIPTGVAVRCTEERSQLANKTRALIRLKAKLMVIAEEQRATEIKEIRGDAVKAEWG 403

Query: 403 QQIRNYVFHPYKLVKDVRTGYETSDIVSVMDGELEPFIKAYLKYKYSMALSTGGVN 459
           QQIRNYVFHPYKLVKDVRTG+ETSDI SVMDG+L+PFIKAYLK+KY++A+++   N
Sbjct: 404 QQIRNYVFHPYKLVKDVRTGHETSDITSVMDGDLDPFIKAYLKHKYTLAMASAVTN 455

[Close]

BLAST of Moc08g12310 vs. TAIR 10
Match: AT5G36170.1 (high chlorophyll fluorescent 109 )

HSP 1 Score: 625.9 bits (1613), Expect = 2.5e-179
Identity = 318/417 (76.26%), Postives = 369/417 (88.49%), Query Frame = 0


Query: 43  PHFPTSLSIRSRF-SIVSFATPESQVGEELDADTREWAMQDFYSLRKEVETTSERVEEIR 102
           P+F +S    SRF S + FA  ES +    + +T EW MQDFY+LRK+VE  S RVEEIR
Sbjct: 44  PNFSSS----SRFNSPIVFAAQESNLSVSNENETSEWLMQDFYTLRKDVEIASARVEEIR 103

Query: 103 NSAGLFQLDQELADLESKAADNALWDDRSKAQQVLVALTDVKDKIKMLTDFKTQVEEAET 162
            SA L QL+QE+ +LESKA D + WDDR+KAQ+ L +L D+KD++++L++FKT VE+AET
Sbjct: 104 ASANLQQLEQEITNLESKATDTSFWDDRTKAQETLSSLNDLKDRMRLLSEFKTMVEDAET 163

Query: 163 IVKLTEEMDSVDTGLLEEAASIIKDLNKALDQFELTQLLSGPYDKEGAVISISAGAGGTD 222
           IVKLTEEMDS D  LLEEA  IIK+LNK+LD+FELTQLLSGPYDKEGAV+ I+AGAGGTD
Sbjct: 164 IVKLTEEMDSTDVSLLEEAMGIIKELNKSLDKFELTQLLSGPYDKEGAVVYITAGAGGTD 223

Query: 223 AQDWADMLLRMYVRWGEKQRYKTRVVEKSAGEEAGIKSATIEIEGRYAYGYISGEKGTHR 282
           AQDWADMLLRMY+RWGEKQRYKT+VVE S GEEAGIKSAT+EIEGRYAYGYISGEKGTHR
Sbjct: 224 AQDWADMLLRMYMRWGEKQRYKTKVVEMSNGEEAGIKSATLEIEGRYAYGYISGEKGTHR 283

Query: 283 IVRQSPFNSKGLRQTSFSGVEVMPLLPDESMDVELPEEDLEISFSRAGGKGGQNVNKVET 342
           IVRQSPFNSKGLRQTSFSGVEVMPLLP+E++ +E+PEEDL+ISF+RAGGKGGQNVNKVET
Sbjct: 284 IVRQSPFNSKGLRQTSFSGVEVMPLLPEEAVGIEIPEEDLDISFTRAGGKGGQNVNKVET 343

Query: 343 AVRITHIPTGVTVRCTEERSQLANKIKALSRLKAKLLVIAEEQRASEIKQIRGDAVKAEW 402
           AVRITHIPTGV VRCTEERSQLANK +AL RLKAKL+VIAEEQRA+EIK+IRGDAVKAEW
Sbjct: 344 AVRITHIPTGVAVRCTEERSQLANKTRALIRLKAKLMVIAEEQRATEIKEIRGDAVKAEW 403

Query: 403 GQQIRNYVFHPYKLVKDVRTGYETSDIVSVMDGELEPFIKAYLKYKYSMALSTGGVN 459
           GQQIRNYVFHPYKLVKDVRTG+ETSDI SVMDG+L+PFIKAYLK+KY++A+++   N
Sbjct: 404 GQQIRNYVFHPYKLVKDVRTGHETSDITSVMDGDLDPFIKAYLKHKYTLAMASAVTN 456

[Close]

BLAST of Moc08g12310 vs. TAIR 10
Match: AT5G36170.3 (high chlorophyll fluorescent 109 )

HSP 1 Score: 461.5 bits (1186), Expect = 8.1e-130
Identity = 236/317 (74.45%), Postives = 275/317 (86.75%), Query Frame = 0


Query: 43  PHFPTSLSIRSRF-SIVSFATPESQVGEELDADTREWAMQDFYSLRKEVETTSERVEEIR 102
           P+F +S    SRF S + FA  ES +    + +T EW MQDFY+LRK+VE  S RVEEIR
Sbjct: 44  PNFSSS----SRFNSPIVFAAQESNLSVSNENETSEWLMQDFYTLRKDVEIASARVEEIR 103

Query: 103 NSAGLFQLDQELADLESKAADNALWDDRSKAQQVLVALTDVKDKIKMLTDFKTQVEEAET 162
            SA L QL+QE+ +LESKA D + WDDR+KAQ+ L +L D+KD++++L++FKT VE+AET
Sbjct: 104 ASANLQQLEQEITNLESKATDTSFWDDRTKAQETLSSLNDLKDRMRLLSEFKTMVEDAET 163

Query: 163 IVKLTEEMDSVDTGLLEEAASIIKDLNKALDQFELTQLLSGPYDKEGAVISISAGAGGTD 222
           IVKLTEEMDS D  LLEEA  IIK+LNK+LD+FELTQLLSGPYDKEGAV+ I+AGAGGTD
Sbjct: 164 IVKLTEEMDSTDVSLLEEAMGIIKELNKSLDKFELTQLLSGPYDKEGAVVYITAGAGGTD 223

Query: 223 AQDWADMLLRMYVRWGEKQRYKTRVVEKSAGEEAGIKSATIEIEGRYAYGYISGEKGTHR 282
           AQDWADMLLRMY+RWGEKQRYKT+VVE S GEEAGIKSAT+EIEGRYAYGYISGEKGTHR
Sbjct: 224 AQDWADMLLRMYMRWGEKQRYKTKVVEMSNGEEAGIKSATLEIEGRYAYGYISGEKGTHR 283

Query: 283 IVRQSPFNSKGLRQTSFSGVEVMPLLPDESMDVELPEEDLEISFSRAGGKGGQNVNKVET 342
           IVRQSPFNSKGLRQTSFSGVEVMPLLP+E++ +E+PEEDL+ISF+RAGGKGGQNVNKVET
Sbjct: 284 IVRQSPFNSKGLRQTSFSGVEVMPLLPEEAVGIEIPEEDLDISFTRAGGKGGQNVNKVET 343

Query: 343 AVRITHIPTGVTVRCTE 359
           AVRITHIPTGV VRCT+
Sbjct: 344 AVRITHIPTGVAVRCTD 356

[Close]

BLAST of Moc08g12310 vs. TAIR 10
Match: AT1G56350.1 (Peptide chain release factor 2 )

HSP 1 Score: 245.4 bits (625), Expect = 9.2e-65
Identity = 139/374 (37.17%), Postives = 216/374 (57.75%), Query Frame = 0


Query: 71  LDADTREWAMQDFYSLRKEVETTSERVEEIRNSAGLFQLDQELADLESKAADNALWDDRS 130
           LD +  +W        +      ++ ++ I+      +L   L  L ++   + LWDD +
Sbjct: 108 LDENEGDW--------KSHAAAIAQSIQVIKRRLQWKKLLVRLKVLSAELNKSDLWDDPT 167

Query: 131 KAQQVLVALTDVKDKIKMLTDFKTQVEEAETIVKLTEEMDSVDTGLLEEAASIIKDLNKA 190
            A ++      +  K+K +  F+ ++ E   ++KL +E +  D+ L  E    + D+ + 
Sbjct: 168 HAGKISREHGSLTGKMKGVMTFERELLEHIDMLKLAKEEN--DSELESETLKALIDMRRV 227

Query: 191 LDQFELTQLLSGPYDKEGAVISISAGAGGTDAQDWADMLLRMYVRWGEKQRYKTRVVEKS 250
             + EL  LLS   D     I + AGAGGT++ DWA M++ MY  W +++++   VV+++
Sbjct: 228 SKEKELEALLSADNDPCSCYIEVQAGAGGTESNDWAAMVMEMYKTWAQRRKFSVTVVDEA 287

Query: 251 AGEEAGIKSATIEIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSGVEVMPLLPDE 310
            GE AGIK ATI++ G YAYGY   E G HR+VR SPF+S   R TSF+ V V+P+L D 
Sbjct: 288 PGEIAGIKRATIKVNGEYAYGYAKAEVGVHRLVRISPFDSGKRRHTSFAAVAVIPILGDG 347

Query: 311 SMDVELPEEDLEISFSRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEERSQLANKIKAL 370
           S  VE+ + DL I   R+GG GGQ+ N  ++AVRI HIPTG+T  C  ERSQ +NK  A+
Sbjct: 348 STRVEINDSDLRIERFRSGGAGGQHANTTDSAVRIVHIPTGITATCQNERSQHSNKASAM 407

Query: 371 SRLKAKLLVIAEEQRASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVRTGYETSDIVS 430
           + L+++L  +   ++ +   Q      +  WG QIR YV HPY++VKD+RT YE SD  S
Sbjct: 408 AVLQSRLDQLEMARQTAMNAQHTQSLTEISWGNQIRTYVLHPYRMVKDLRTNYEVSDPDS 467

Query: 431 VMDGELEPFIKAYL 445
           V++G+L+ FI ++L
Sbjct: 468 VLEGDLDGFILSFL 471

[Close]

BLAST of Moc08g12310 vs. TAIR 10
Match: AT3G57190.1 (peptide chain release factor, putative )

HSP 1 Score: 215.7 bits (548), Expect = 7.8e-56
Identity = 137/400 (34.25%), Postives = 232/400 (58.00%), Query Frame = 0


Query: 53  SRFSIVSFATPESQVGEELDADTREWAMQDFYSLRKEVETTSERVEEIRNSAGLFQLDQE 112
           SRF I S     S   +++D   ++  +   +SL+K+++    + E     A   + +Q 
Sbjct: 22  SRFLIFSVRASSSM--DDMDTVYKQLGL---FSLKKKIKDVVLKAEMFAPDALELEEEQW 81

Query: 113 LADLESKAADNALWDDRSKAQQVLVALTDVKDKIKMLTDFKTQVEEAETIVKLTEEMDSV 172
           +   E+    + LWDD +K+ ++L+ L D    +  L D K + EEA+ I++L  EMD++
Sbjct: 82  IKQEETMRYFD-LWDDPAKSDEILLKLADRAKAVDSLKDLKYKAEEAKLIIQL-GEMDAI 141

Query: 173 DTGLLEEAASIIKDLNKALDQFELTQLLSGPYDKEGAVISISAGAGGTDAQDWADMLLRM 232
           D  L E+A     D++++L  +E+++LL   YD EGA + I +G+ G  +Q W + ++ M
Sbjct: 142 DYSLFEQAYDSSLDVSRSLHHYEMSKLLRDQYDAEGACMIIKSGSPGAKSQIWTEQVVSM 201

Query: 233 YVRWGEKQRYKTRVVEKSA--GEEAGIKSATIEIEGRYAYGYISGEKGTHRIVRQSPFNS 292
           Y++W E+     RV EK +    ++G+ SATIE E  +AYGY+ GE+G HR++  S  N 
Sbjct: 202 YIKWAERLGQNARVAEKCSLLSNKSGVSSATIEFEFEFAYGYLLGERGVHRLIISSTSN- 261

Query: 293 KGLRQTSFSGVEVMPLLPDESMDVELPEEDLEISFSRAGGKGGQNVNKVETAVRITHIPT 352
               +   + V+++PL    S D E+ E DL +S+        ++    E  V I HIP+
Sbjct: 262 ----EECSATVDIIPLFLRASPDFEVKEGDLIVSY-----PAKEDHKIAENMVCIHHIPS 321

Query: 353 GVTVRCTEERSQLANKIKALSRLKAKLLVIAEEQRASEIKQIRGDAVKAEWGQQIRNYVF 412
           GVT++ + ER++ AN+IKAL+RLKAKLLVIA+EQ+ S++ +I    +  E  ++ R+YV 
Sbjct: 322 GVTLQSSGERNRFANRIKALNRLKAKLLVIAKEQKVSDVNKIDSKNI-LEPREETRSYVS 381

Query: 413 HPYKLVKDVRTGYETSDIVSVMDGELEPFIKAYLKYKYSM 451
             +K+V D +TG E  D+ SV+DG + P + A++  + S+
Sbjct: 382 KGHKMVVDRKTGLEILDLKSVLDGNIGPLLGAHISMRRSI 403

The following BLAST results are available for this feature:

BLAST of Moc08g12310 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022148293.1	9.3e-253	100.00	peptide chain release factor PrfB1, chloroplastic [Momordica charantia]	[more]
XP_038887337.1	3.7e-225	92.31	peptide chain release factor PrfB1, chloroplastic-like isoform X1 [Benincasa his...	[more]
XP_022949524.1	5.3e-224	90.39	peptide chain release factor PrfB1, chloroplastic isoform X2 [Cucurbita moschata...	[more]
XP_022998734.1	9.1e-224	90.39	peptide chain release factor PrfB1, chloroplastic isoform X2 [Cucurbita maxima]	[more]
XP_023525877.1	4.5e-223	89.96	peptide chain release factor PrfB1, chloroplastic isoform X2 [Cucurbita pepo sub...	[more]

BLAST of Moc08g12310 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LVY0	3.5e-178	76.26	Peptide chain release factor PrfB1, chloroplastic OS=Arabidopsis thaliana OX=370...	[more]
P74476	2.5e-115	58.73	Peptide chain release factor 2 OS=Synechocystis sp. (strain PCC 6803 / Kazusa) O...	[more]
B7K0A6	3.0e-113	57.78	Peptide chain release factor 2 OS=Rippkaea orientalis (strain PCC 8801) OX=41431...	[more]
Q7U3W6	1.9e-107	56.04	Peptide chain release factor 2 OS=Synechococcus sp. (strain WH8102) OX=84588 GN=...	[more]
O67695	9.8e-88	45.86	Peptide chain release factor 2 OS=Aquifex aeolicus (strain VF5) OX=224324 GN=prf...	[more]

BLAST of Moc08g12310 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1D3Q2	4.5e-253	100.00	peptide chain release factor PrfB1, chloroplastic OS=Momordica charantia OX=3673...	[more]
A0A6J1GD28	2.6e-224	90.39	peptide chain release factor PrfB1, chloroplastic isoform X2 OS=Cucurbita moscha...	[more]
A0A6J1KF53	4.4e-224	90.39	peptide chain release factor PrfB1, chloroplastic isoform X2 OS=Cucurbita maxima...	[more]
A0A0A0LY01	1.3e-223	90.55	RF_PROK_I domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G652260 P...	[more]
Q6ZY50	6.3e-223	90.33	Putative translation releasing factor 2 OS=Cucumis sativus OX=3659 GN=rf2 PE=2 S...	[more]

BLAST of Moc08g12310 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G36170.2	6.6e-180	76.20	high chlorophyll fluorescent 109	[more]
AT5G36170.1	2.5e-179	76.26	high chlorophyll fluorescent 109	[more]
AT5G36170.3	8.1e-130	74.45	high chlorophyll fluorescent 109	[more]
AT1G56350.1	9.2e-65	37.17	Peptide chain release factor 2	[more]
AT3G57190.1	7.8e-56	34.25	peptide chain release factor, putative	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 177..197
None	No IPR available	GENE3D	1.20.58.410	Release factor	coord: 86..204 e-value: 8.4E-12 score: 47.2
None	No IPR available	GENE3D	3.30.160.20		coord: 307..403 e-value: 4.5E-88 score: 296.0
None	No IPR available	GENE3D	3.30.70.1660		coord: 211..441 e-value: 4.5E-88 score: 296.0
None	No IPR available	PANTHER	PTHR43116:SF3	PEPTIDE CHAIN RELEASE FACTOR PRFB1, CHLOROPLASTIC-RELATED	coord: 63..451
None	No IPR available	PANTHER	PTHR43116	PEPTIDE CHAIN RELEASE FACTOR 2	coord: 63..451
None	No IPR available	SUPERFAMILY	75620	Release factor	coord: 109..449
IPR005139	Peptide chain release factor	SMART	SM00937	PCRF_a_2	coord: 164..276 e-value: 1.6E-42 score: 157.3
IPR005139	Peptide chain release factor	PFAM	PF03462	PCRF	coord: 110..301 e-value: 6.5E-52 score: 176.2
IPR000352	Peptide chain release factor class I	PFAM	PF00472	RF-1	coord: 312..420 e-value: 4.1E-35 score: 120.2
IPR000352	Peptide chain release factor class I	PROSITE	PS00745	RF_PROK_I	coord: 327..343
IPR004374	Peptide chain release factor 2	TIGRFAM	TIGR00020	TIGR00020	coord: 84..447 e-value: 3.4E-124 score: 412.4
IPR004374	Peptide chain release factor 2	HAMAP	MF_00094	Rel_fac_2	coord: 82..448 score: 36.611427

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc08g12310.1	Moc08g12310.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006415	translational termination
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0016149	translation release factor activity, codon specific
molecular_function	GO:0003747	translation release factor activity