Moc07g05830 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc07g05830
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description30S ribosomal protein S12, chloroplastic
Locationchr7: 4873078 .. 4873727 (-)
RNA-Seq ExpressionMoc07g05830
SyntenyMoc07g05830
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAATGAAAACGTGACTGAATTGGTCCTGGTCACTCTTCGAGATGGCATGGAAGAAGGAAGGAGATTCTCGAACGAGGAAAAAGATCCAACGACTTTGAAAGAATTGAACGAGGAGCCGTATGAGGTGAAAATCTCATGTATGGTCCTGTCGAGTGGCAGTAAGGGTGACTTATCTGTTAACTTTTCCACTATCACCCCCAAAAAACCAAACTCTGCTTTAGGTAAAGTTGCCAGAGTACGCTTAACCTCGGGATTTGAAATCACTGCTTATATACCTGGTATTGGCCATAATTTACAAGAACATTCTGTAGTCTTAGTAAAAGGGAGAAGGGTTAAGGACCTCTTGAAAAAAGCGAGCAGCAAGCTGAAAAAGCTCTTTCCCCTTTGAATAATAATAACATCAGCTTCATAGCTCCAACCTATACAAGGGGTAAGCCAAAACTCGTTGAAGGAAGGGCTTCATAGCCTTACCTTATTATTAGAGTGACTGGGAAGTCCGCTTCAGTCATTTGAGTCTTTGACAACCACTCTCTCCTGTGAAACTCCTCCTTGTTCTATGATTGATCTAACCATTTTCTTTAGGCAGAAGCTGAAAGTTGGTTCACGCGGGCACAAACGAGCACCGAATGCAACAACAGGGAACTGTGA

mRNA sequence

ATGAATGAAAACGTGACTGAATTGGTCCTGGTCACTCTTCGAGATGGCATGGAAGAAGGAAGGAGATTCTCGAACGAGGAAAAAGATCCAACGACTTTGAAAGAATTGAACGAGGAGCCGTATGAGGTGAAAATCTCATGTATGGTCCTGTCGAGTGGCAGTAAGGGTGACTTATCTGTTAACTTTTCCACTATCACCCCCAAAAAACCAAACTCTGCTTTAGGTAAAGTTGCCAGAGTACGCTTAACCTCGGGATTTGAAATCACTGCTTATATACCTGAAGCTGAAAGTTGGTTCACGCGGGCACAAACGAGCACCGAATGCAACAACAGGGAACTGTGA

Coding sequence (CDS)

ATGAATGAAAACGTGACTGAATTGGTCCTGGTCACTCTTCGAGATGGCATGGAAGAAGGAAGGAGATTCTCGAACGAGGAAAAAGATCCAACGACTTTGAAAGAATTGAACGAGGAGCCGTATGAGGTGAAAATCTCATGTATGGTCCTGTCGAGTGGCAGTAAGGGTGACTTATCTGTTAACTTTTCCACTATCACCCCCAAAAAACCAAACTCTGCTTTAGGTAAAGTTGCCAGAGTACGCTTAACCTCGGGATTTGAAATCACTGCTTATATACCTGAAGCTGAAAGTTGGTTCACGCGGGCACAAACGAGCACCGAATGCAACAACAGGGAACTGTGA

Protein sequence

MNENVTELVLVTLRDGMEEGRRFSNEEKDPTTLKELNEEPYEVKISCMVLSSGSKGDLSVNFSTITPKKPNSALGKVARVRLTSGFEITAYIPEAESWFTRAQTSTECNNREL
Homology
BLAST of Moc07g05830 vs. NCBI nr
Match: XP_022932068.1 (uncharacterized protein LOC111438394 [Cucurbita moschata] >XP_023520570.1 uncharacterized protein LOC111783982 [Cucurbita pepo subsp. pepo] >XP_023520572.1 uncharacterized protein LOC111783984 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 170.6 bits (431), Expect = 7.6e-39
Identity = 88/93 (94.62%), Postives = 88/93 (94.62%), Query Frame = 0

Query: 1  MNENVTELVLVTLRDGMEEGRRFSNEEKDPTTLKELNEEPYEVKISCMVLSSGSKGDLSV 60
          MNENVTELV VTLRDGMEEGRRFSNEEKDP T KELNEEPYEVKISC VLSSGSKGDLSV
Sbjct: 1  MNENVTELVPVTLRDGMEEGRRFSNEEKDPMTSKELNEEPYEVKISCTVLSSGSKGDLSV 60

Query: 61 NFSTITPKKPNSALGKVARVRLTSGFEITAYIP 94
          NFSTITPKKPNSAL KVARVRLTSGFEITAYIP
Sbjct: 61 NFSTITPKKPNSALRKVARVRLTSGFEITAYIP 93

BLAST of Moc07g05830 vs. NCBI nr
Match: XP_038902483.1 (30S ribosomal protein S12, chloroplastic-like [Benincasa hispida])

HSP 1 Score: 168.3 bits (425), Expect = 3.8e-38
Identity = 87/93 (93.55%), Postives = 88/93 (94.62%), Query Frame = 0

Query: 1  MNENVTELVLVTLRDGMEEGRRFSNEEKDPTTLKELNEEPYEVKISCMVLSSGSKGDLSV 60
          MNENVTELVLVTLRDGMEEGRRFSNEEKDP T KELNEEPYEVKIS  VLSSGSKGDLS+
Sbjct: 1  MNENVTELVLVTLRDGMEEGRRFSNEEKDPMTSKELNEEPYEVKISRTVLSSGSKGDLSI 60

Query: 61 NFSTITPKKPNSALGKVARVRLTSGFEITAYIP 94
          NFSTITPKKPNSAL KVARVRLTSGFEITAYIP
Sbjct: 61 NFSTITPKKPNSALRKVARVRLTSGFEITAYIP 93

BLAST of Moc07g05830 vs. NCBI nr
Match: XP_038896566.1 (30S ribosomal protein S12, chloroplastic-like [Benincasa hispida])

HSP 1 Score: 157.1 bits (396), Expect = 8.7e-35
Identity = 83/93 (89.25%), Postives = 84/93 (90.32%), Query Frame = 0

Query: 1  MNENVTELVLVTLRDGMEEGRRFSNEEKDPTTLKELNEEPYEVKISCMVLSSGSKGDLSV 60
          MNENVT+LV VTLRDGMEEGRRFSNEEKDP T KELNEEPYEVKIS  VLSSGSKGDLSV
Sbjct: 1  MNENVTKLVPVTLRDGMEEGRRFSNEEKDPMTSKELNEEPYEVKISRTVLSSGSKGDLSV 60

Query: 61 NFSTITPKKPNSALGKVARVRLTSGFEITAYIP 94
          NFSTITPK  NSAL KVARVRLTSGFEI AYIP
Sbjct: 61 NFSTITPKNQNSALRKVARVRLTSGFEIIAYIP 93

BLAST of Moc07g05830 vs. NCBI nr
Match: KAF1876942.1 (hypothetical protein Lal_00033867 [Lupinus albus])

HSP 1 Score: 156.8 bits (395), Expect = 1.1e-34
Identity = 81/92 (88.04%), Postives = 85/92 (92.39%), Query Frame = 0

Query: 2   NENVTELVLVTLRDGMEEGRRFSNEEKDPTTLKELNEEPYEVKISCMVLSSGSKGDLSVN 61
           +ENVT+LVLVTLRDG+EEG RFSNEEKDP T KELN+E YEVKISC VL SGSKGDLSVN
Sbjct: 80  HENVTKLVLVTLRDGVEEGGRFSNEEKDPMTSKELNKESYEVKISCSVLLSGSKGDLSVN 139

Query: 62  FSTITPKKPNSALGKVARVRLTSGFEITAYIP 94
           FSTITPKKPNSAL KVARVRLTSGFEITAYIP
Sbjct: 140 FSTITPKKPNSALRKVARVRLTSGFEITAYIP 171

BLAST of Moc07g05830 vs. NCBI nr
Match: RWR98012.1 (LOW QUALITY PROTEIN: 30S ribosomal protein S12, chloroplastic [Cinnamomum micranthum f. kanehirae])

HSP 1 Score: 156.0 bits (393), Expect = 1.9e-34
Identity = 82/92 (89.13%), Postives = 85/92 (92.39%), Query Frame = 0

Query: 2  NENVTELVLVTLRDGMEEGRRFSNEEKDPTTLKELNEEPYEVKISCMVLSSGSKGDLSVN 61
          +ENVTELVLVTLRDG+EEGRRFSNEEKDP T KELNEEPYEVKISC   +S SKGDLSVN
Sbjct: 8  HENVTELVLVTLRDGVEEGRRFSNEEKDPITSKELNEEPYEVKISC---TSDSKGDLSVN 67

Query: 62 FSTITPKKPNSALGKVARVRLTSGFEITAYIP 94
          FSTITPKKPNSAL KVARVRLTSGFEITAYIP
Sbjct: 68 FSTITPKKPNSALRKVARVRLTSGFEITAYIP 96

BLAST of Moc07g05830 vs. ExPASy Swiss-Prot
Match: A4GYN8 (30S ribosomal protein S12-B, chloroplastic OS=Populus trichocarpa OX=3694 GN=rps12-B PE=3 SV=1)

HSP 1 Score: 84.0 bits (206), Expect = 1.2e-15
Identity = 42/48 (87.50%), Postives = 44/48 (91.67%), Query Frame = 0

Query: 46 SCMVLSSGSKGDLSVNFSTITPKKPNSALGKVARVRLTSGFEITAYIP 94
          +C  + SGSKGDLSVNFSTITPKKPNSAL KVARVRLTSGFEITAYIP
Sbjct: 33 TCTRVYSGSKGDLSVNFSTITPKKPNSALRKVARVRLTSGFEITAYIP 80

BLAST of Moc07g05830 vs. ExPASy Swiss-Prot
Match: A4QJX7 (30S ribosomal protein S12-B, chloroplastic OS=Olimarabidopsis pumila OX=74718 GN=rps12-B PE=3 SV=1)

HSP 1 Score: 62.4 bits (150), Expect = 3.8e-09
Identity = 31/39 (79.49%), Postives = 34/39 (87.18%), Query Frame = 0

Query: 55 KGDLSVNFSTITPKKPNSALGKVARVRLTSGFEITAYIP 94
          +G  +  +STITPKKPNSAL KVARVRLTSGFEITAYIP
Sbjct: 31 RGTCTRVYSTITPKKPNSALRKVARVRLTSGFEITAYIP 69

BLAST of Moc07g05830 vs. ExPASy Swiss-Prot
Match: A4QJV5 (30S ribosomal protein S12-A, chloroplastic OS=Olimarabidopsis pumila OX=74718 GN=rps12-A PE=3 SV=1)

HSP 1 Score: 59.3 bits (142), Expect = 3.2e-08
Identity = 29/30 (96.67%), Postives = 29/30 (96.67%), Query Frame = 0

Query: 64 TITPKKPNSALGKVARVRLTSGFEITAYIP 94
          TITPKKPNSAL KVARVRLTSGFEITAYIP
Sbjct: 39 TITPKKPNSALRKVARVRLTSGFEITAYIP 68

BLAST of Moc07g05830 vs. ExPASy Swiss-Prot
Match: A4GYT5 (30S ribosomal protein S12-A, chloroplastic OS=Populus trichocarpa OX=3694 GN=rps12-A PE=3 SV=1)

HSP 1 Score: 59.3 bits (142), Expect = 3.2e-08
Identity = 29/30 (96.67%), Postives = 29/30 (96.67%), Query Frame = 0

Query: 64 TITPKKPNSALGKVARVRLTSGFEITAYIP 94
          TITPKKPNSAL KVARVRLTSGFEITAYIP
Sbjct: 39 TITPKKPNSALRKVARVRLTSGFEITAYIP 68

BLAST of Moc07g05830 vs. ExPASy Swiss-Prot
Match: Q3V510 (30S ribosomal protein S12, chloroplastic OS=Acorus calamus OX=4465 GN=rps12-A PE=3 SV=1)

HSP 1 Score: 59.3 bits (142), Expect = 3.2e-08
Identity = 29/30 (96.67%), Postives = 29/30 (96.67%), Query Frame = 0

Query: 64 TITPKKPNSALGKVARVRLTSGFEITAYIP 94
          TITPKKPNSAL KVARVRLTSGFEITAYIP
Sbjct: 39 TITPKKPNSALRKVARVRLTSGFEITAYIP 68

BLAST of Moc07g05830 vs. ExPASy TrEMBL
Match: A0A6J1F0L1 (uncharacterized protein LOC111438394 OS=Cucurbita moschata OX=3662 GN=LOC111438394 PE=3 SV=1)

HSP 1 Score: 170.6 bits (431), Expect = 3.7e-39
Identity = 88/93 (94.62%), Postives = 88/93 (94.62%), Query Frame = 0

Query: 1  MNENVTELVLVTLRDGMEEGRRFSNEEKDPTTLKELNEEPYEVKISCMVLSSGSKGDLSV 60
          MNENVTELV VTLRDGMEEGRRFSNEEKDP T KELNEEPYEVKISC VLSSGSKGDLSV
Sbjct: 1  MNENVTELVPVTLRDGMEEGRRFSNEEKDPMTSKELNEEPYEVKISCTVLSSGSKGDLSV 60

Query: 61 NFSTITPKKPNSALGKVARVRLTSGFEITAYIP 94
          NFSTITPKKPNSAL KVARVRLTSGFEITAYIP
Sbjct: 61 NFSTITPKKPNSALRKVARVRLTSGFEITAYIP 93

BLAST of Moc07g05830 vs. ExPASy TrEMBL
Match: A0A6A5N1Q8 (Uncharacterized protein OS=Lupinus albus OX=3870 GN=Lal_00033867 PE=3 SV=1)

HSP 1 Score: 156.8 bits (395), Expect = 5.5e-35
Identity = 81/92 (88.04%), Postives = 85/92 (92.39%), Query Frame = 0

Query: 2   NENVTELVLVTLRDGMEEGRRFSNEEKDPTTLKELNEEPYEVKISCMVLSSGSKGDLSVN 61
           +ENVT+LVLVTLRDG+EEG RFSNEEKDP T KELN+E YEVKISC VL SGSKGDLSVN
Sbjct: 80  HENVTKLVLVTLRDGVEEGGRFSNEEKDPMTSKELNKESYEVKISCSVLLSGSKGDLSVN 139

Query: 62  FSTITPKKPNSALGKVARVRLTSGFEITAYIP 94
           FSTITPKKPNSAL KVARVRLTSGFEITAYIP
Sbjct: 140 FSTITPKKPNSALRKVARVRLTSGFEITAYIP 171

BLAST of Moc07g05830 vs. ExPASy TrEMBL
Match: A0A443Q5G0 (30S ribosomal protein S12, chloroplastic OS=Cinnamomum micranthum f. kanehirae OX=337451 GN=CKAN_02772100 PE=3 SV=1)

HSP 1 Score: 156.0 bits (393), Expect = 9.4e-35
Identity = 82/92 (89.13%), Postives = 85/92 (92.39%), Query Frame = 0

Query: 2  NENVTELVLVTLRDGMEEGRRFSNEEKDPTTLKELNEEPYEVKISCMVLSSGSKGDLSVN 61
          +ENVTELVLVTLRDG+EEGRRFSNEEKDP T KELNEEPYEVKISC   +S SKGDLSVN
Sbjct: 8  HENVTELVLVTLRDGVEEGRRFSNEEKDPITSKELNEEPYEVKISC---TSDSKGDLSVN 67

Query: 62 FSTITPKKPNSALGKVARVRLTSGFEITAYIP 94
          FSTITPKKPNSAL KVARVRLTSGFEITAYIP
Sbjct: 68 FSTITPKKPNSALRKVARVRLTSGFEITAYIP 96

BLAST of Moc07g05830 vs. ExPASy TrEMBL
Match: A0A3S3NSS0 (30S ribosomal protein S12, chloroplastic OS=Cinnamomum micranthum f. kanehirae OX=337451 GN=CKAN_02749900 PE=3 SV=1)

HSP 1 Score: 156.0 bits (393), Expect = 9.4e-35
Identity = 82/92 (89.13%), Postives = 85/92 (92.39%), Query Frame = 0

Query: 2  NENVTELVLVTLRDGMEEGRRFSNEEKDPTTLKELNEEPYEVKISCMVLSSGSKGDLSVN 61
          +ENVTELVLVTLRDG+EEGRRFSNEEKDP T KELNEEPYEVKISC   +S SKGDLSVN
Sbjct: 8  HENVTELVLVTLRDGVEEGRRFSNEEKDPITSKELNEEPYEVKISC---TSDSKGDLSVN 67

Query: 62 FSTITPKKPNSALGKVARVRLTSGFEITAYIP 94
          FSTITPKKPNSAL KVARVRLTSGFEITAYIP
Sbjct: 68 FSTITPKKPNSALRKVARVRLTSGFEITAYIP 96

BLAST of Moc07g05830 vs. ExPASy TrEMBL
Match: A0A6P6GKJ1 (uncharacterized protein LOC112488768 OS=Ziziphus jujuba OX=326968 GN=LOC112488768 PE=3 SV=1)

HSP 1 Score: 147.9 bits (372), Expect = 2.6e-32
Identity = 73/93 (78.49%), Postives = 82/93 (88.17%), Query Frame = 0

Query: 1  MNENVTELVLVTLRDGMEEGRRFSNEEKDPTTLKELNEEPYEVKISCMVLSSGSKGDLSV 60
          MNENVTELVLVTL++G+EEG+RFSNEE D  T KELNEEPYE+KISCMVL +G KGDLS+
Sbjct: 1  MNENVTELVLVTLQNGVEEGKRFSNEENDRMTSKELNEEPYEIKISCMVLQNGIKGDLSI 60

Query: 61 NFSTITPKKPNSALGKVARVRLTSGFEITAYIP 94
          NFS I PKKPNSAL KVARVRLT GFE+T +IP
Sbjct: 61 NFSIIIPKKPNSALSKVARVRLTFGFEMTTFIP 93

BLAST of Moc07g05830 vs. TAIR 10
Match: ATCG00065.1 (ribosomal protein S12A )

HSP 1 Score: 59.3 bits (142), Expect = 2.3e-09
Identity = 29/30 (96.67%), Postives = 29/30 (96.67%), Query Frame = 0

Query: 64 TITPKKPNSALGKVARVRLTSGFEITAYIP 94
          TITPKKPNSAL KVARVRLTSGFEITAYIP
Sbjct: 39 TITPKKPNSALRKVARVRLTSGFEITAYIP 68

BLAST of Moc07g05830 vs. TAIR 10
Match: ATCG01230.1 (ribosomal protein S12B )

HSP 1 Score: 59.3 bits (142), Expect = 2.3e-09
Identity = 29/30 (96.67%), Postives = 29/30 (96.67%), Query Frame = 0

Query: 64 TITPKKPNSALGKVARVRLTSGFEITAYIP 94
          TITPKKPNSAL KVARVRLTSGFEITAYIP
Sbjct: 39 TITPKKPNSALRKVARVRLTSGFEITAYIP 68

BLAST of Moc07g05830 vs. TAIR 10
Match: ATCG00905.1 (ribosomal protein S12C )

HSP 1 Score: 59.3 bits (142), Expect = 2.3e-09
Identity = 29/30 (96.67%), Postives = 29/30 (96.67%), Query Frame = 0

Query: 64 TITPKKPNSALGKVARVRLTSGFEITAYIP 94
          TITPKKPNSAL KVARVRLTSGFEITAYIP
Sbjct: 39 TITPKKPNSALRKVARVRLTSGFEITAYIP 68

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022932068.17.6e-3994.62uncharacterized protein LOC111438394 [Cucurbita moschata] >XP_023520570.1 unchar... [more]
XP_038902483.13.8e-3893.5530S ribosomal protein S12, chloroplastic-like [Benincasa hispida][more]
XP_038896566.18.7e-3589.2530S ribosomal protein S12, chloroplastic-like [Benincasa hispida][more]
KAF1876942.11.1e-3488.04hypothetical protein Lal_00033867 [Lupinus albus][more]
RWR98012.11.9e-3489.13LOW QUALITY PROTEIN: 30S ribosomal protein S12, chloroplastic [Cinnamomum micran... [more]
Match NameE-valueIdentityDescription
A4GYN81.2e-1587.5030S ribosomal protein S12-B, chloroplastic OS=Populus trichocarpa OX=3694 GN=rps... [more]
A4QJX73.8e-0979.4930S ribosomal protein S12-B, chloroplastic OS=Olimarabidopsis pumila OX=74718 GN... [more]
A4QJV53.2e-0896.6730S ribosomal protein S12-A, chloroplastic OS=Olimarabidopsis pumila OX=74718 GN... [more]
A4GYT53.2e-0896.6730S ribosomal protein S12-A, chloroplastic OS=Populus trichocarpa OX=3694 GN=rps... [more]
Q3V5103.2e-0896.6730S ribosomal protein S12, chloroplastic OS=Acorus calamus OX=4465 GN=rps12-A PE... [more]
Match NameE-valueIdentityDescription
A0A6J1F0L13.7e-3994.62uncharacterized protein LOC111438394 OS=Cucurbita moschata OX=3662 GN=LOC1114383... [more]
A0A6A5N1Q85.5e-3588.04Uncharacterized protein OS=Lupinus albus OX=3870 GN=Lal_00033867 PE=3 SV=1[more]
A0A443Q5G09.4e-3589.1330S ribosomal protein S12, chloroplastic OS=Cinnamomum micranthum f. kanehirae O... [more]
A0A3S3NSS09.4e-3589.1330S ribosomal protein S12, chloroplastic OS=Cinnamomum micranthum f. kanehirae O... [more]
A0A6P6GKJ12.6e-3278.49uncharacterized protein LOC112488768 OS=Ziziphus jujuba OX=326968 GN=LOC11248876... [more]
Match NameE-valueIdentityDescription
ATCG00065.12.3e-0996.67ribosomal protein S12A [more]
ATCG01230.12.3e-0996.67ribosomal protein S12B [more]
ATCG00905.12.3e-0996.67ribosomal protein S12C [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006032Ribosomal protein S12/S23PRINTSPR01034RIBOSOMALS12coord: 67..82
score: 85.24
coord: 83..102
score: 35.62
IPR006032Ribosomal protein S12/S23PFAMPF00164Ribosom_S12_S23coord: 58..96
e-value: 1.4E-11
score: 44.0
IPR006032Ribosomal protein S12/S23PANTHERPTHR1165230S RIBOSOMAL PROTEIN S12 FAMILY MEMBERcoord: 35..94
NoneNo IPR availableGENE3D2.40.50.140coord: 8..105
e-value: 2.9E-15
score: 58.0
NoneNo IPR availablePANTHERPTHR11652:SF5230S RIBOSOMAL PROTEIN S12-Bcoord: 35..94
IPR012340Nucleic acid-binding, OB-foldSUPERFAMILY50249Nucleic acid-binding proteinscoord: 61..98

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc07g05830.1Moc07g05830.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006412 translation
cellular_component GO:0015935 small ribosomal subunit
cellular_component GO:0005840 ribosome
molecular_function GO:0003735 structural constituent of ribosome