Moc07g04180 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc07g04180
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	rRNA N-glycosidase
Location	chr7: 3643143 .. 3644096 (-)
RNA-Seq Expression	Moc07g04180
Synteny	Moc07g04180

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGACCACATATTACAGTGTCATGAAGAAAATGGTGGCTATAAAAAGCACATCCTTGAGATGTTCCAACGGCATCCAATCCAATCCAACCCAAGTTTCGAAAACATGAGCTACTCCAACTCAACCCTTTGTTGCCTACTCCTCGCCATCATCCTCGTAGCCGCTCCGCACACAGCGGAGGGCCAGCGCGAGATCACCTTCACCACAAACATAAACACGTACGCCTCAGAGCTGAATGCTTTCCGACAAGCCGTACGTACCACCAACAAAAGGTTCGACTACTTTCTCCTGCCTCTCAGAACCGCCGTCTCCGGCACAAACCGCTACAGAGAAATCAACCTCAGAAACTCCCGCGGCGTCGCCGTCAAGCTGGCCATAGACATCGTCAACGTCTACGTCCTTGCCTACCACACCTCCAACCCAAGCTGCCGCGGATCCTACTTTTTCAACGACGCCGATGCCCGCGCTGCCATGTCCTTCGTATTCCGAAATTCTGGGGAACAAAGAGTGCTCCCATTCGACGGCAGCTACGGCGACCTCGCAGCAAACGGAGGCAGCCGCCGGAATACCATTCTCGGCCTCTCACAGCTCAGCGACCTGCTTCCGGCGCTGTACGACTGCCGGCGGCCGGGCGACACTTACGACCGCATCGCCACAGCGTTGGTGCTGGCCATTCAGACCATCTCCGAGGGCGCCCGGTTCAAGGTGGTTCAGGACGCGGTTCGGCGGAATTCCAGACCGAGCGAACAAGTCATGGATTTCCAGAATAGTTGGCAGACTATCAGCAACGCGATTCAGGAAGCCAACACTAGAACTGGGAATTTCCAGAATAACATTACGCTTCTCAATAATCAGGGCGTTAGAGTTAGTGTGGGGAATGTTGATCACAATCTTGTAAGAGGCAACGTCAAGCTGCTGCTCACTATGCAGCCTCGAAGTTATGCTGCCGTCTAA

mRNA sequence

ATGGACCACATATTACAGTGTCATGAAGAAAATGGTGGCTATAAAAAGCACATCCTTGAGATGTTCCAACGGCATCCAATCCAATCCAACCCAAGTTTCGAAAACATGAGCTACTCCAACTCAACCCTTTGTTGCCTACTCCTCGCCATCATCCTCGTAGCCGCTCCGCACACAGCGGAGGGCCAGCGCGAGATCACCTTCACCACAAACATAAACACGTACGCCTCAGAGCTGAATGCTTTCCGACAAGCCGTACGTACCACCAACAAAAGGTTCGACTACTTTCTCCTGCCTCTCAGAACCGCCGTCTCCGGCACAAACCGCTACAGAGAAATCAACCTCAGAAACTCCCGCGGCGTCGCCGTCAAGCTGGCCATAGACATCGTCAACGTCTACGTCCTTGCCTACCACACCTCCAACCCAAGCTGCCGCGGATCCTACTTTTTCAACGACGCCGATGCCCGCGCTGCCATGTCCTTCGTATTCCGAAATTCTGGGGAACAAAGAGTGCTCCCATTCGACGGCAGCTACGGCGACCTCGCAGCAAACGGAGGCAGCCGCCGGAATACCATTCTCGGCCTCTCACAGCTCAGCGACCTGCTTCCGGCGCTGTACGACTGCCGGCGGCCGGGCGACACTTACGACCGCATCGCCACAGCGTTGGTGCTGGCCATTCAGACCATCTCCGAGGGCGCCCGGTTCAAGGTGGTTCAGGACGCGGTTCGGCGGAATTCCAGACCGAGCGAACAAGTCATGGATTTCCAGAATAGTTGGCAGACTATCAGCAACGCGATTCAGGAAGCCAACACTAGAACTGGGAATTTCCAGAATAACATTACGCTTCTCAATAATCAGGGCGTTAGAGTTAGTGTGGGGAATGTTGATCACAATCTTGTAAGAGGCAACGTCAAGCTGCTGCTCACTATGCAGCCTCGAAGTTATGCTGCCGTCTAA

Coding sequence (CDS)

ATGGACCACATATTACAGTGTCATGAAGAAAATGGTGGCTATAAAAAGCACATCCTTGAGATGTTCCAACGGCATCCAATCCAATCCAACCCAAGTTTCGAAAACATGAGCTACTCCAACTCAACCCTTTGTTGCCTACTCCTCGCCATCATCCTCGTAGCCGCTCCGCACACAGCGGAGGGCCAGCGCGAGATCACCTTCACCACAAACATAAACACGTACGCCTCAGAGCTGAATGCTTTCCGACAAGCCGTACGTACCACCAACAAAAGGTTCGACTACTTTCTCCTGCCTCTCAGAACCGCCGTCTCCGGCACAAACCGCTACAGAGAAATCAACCTCAGAAACTCCCGCGGCGTCGCCGTCAAGCTGGCCATAGACATCGTCAACGTCTACGTCCTTGCCTACCACACCTCCAACCCAAGCTGCCGCGGATCCTACTTTTTCAACGACGCCGATGCCCGCGCTGCCATGTCCTTCGTATTCCGAAATTCTGGGGAACAAAGAGTGCTCCCATTCGACGGCAGCTACGGCGACCTCGCAGCAAACGGAGGCAGCCGCCGGAATACCATTCTCGGCCTCTCACAGCTCAGCGACCTGCTTCCGGCGCTGTACGACTGCCGGCGGCCGGGCGACACTTACGACCGCATCGCCACAGCGTTGGTGCTGGCCATTCAGACCATCTCCGAGGGCGCCCGGTTCAAGGTGGTTCAGGACGCGGTTCGGCGGAATTCCAGACCGAGCGAACAAGTCATGGATTTCCAGAATAGTTGGCAGACTATCAGCAACGCGATTCAGGAAGCCAACACTAGAACTGGGAATTTCCAGAATAACATTACGCTTCTCAATAATCAGGGCGTTAGAGTTAGTGTGGGGAATGTTGATCACAATCTTGTAAGAGGCAACGTCAAGCTGCTGCTCACTATGCAGCCTCGAAGTTATGCTGCCGTCTAA

Protein sequence

MDHILQCHEENGGYKKHILEMFQRHPIQSNPSFENMSYSNSTLCCLLLAIILVAAPHTAEGQREITFTTNINTYASELNAFRQAVRTTNKRFDYFLLPLRTAVSGTNRYREINLRNSRGVAVKLAIDIVNVYVLAYHTSNPSCRGSYFFNDADARAAMSFVFRNSGEQRVLPFDGSYGDLAANGGSRRNTILGLSQLSDLLPALYDCRRPGDTYDRIATALVLAIQTISEGARFKVVQDAVRRNSRPSEQVMDFQNSWQTISNAIQEANTRTGNFQNNITLLNNQGVRVSVGNVDHNLVRGNVKLLLTMQPRSYAAV

Homology

[Close]

BLAST of Moc07g04180 vs. NCBI nr
Match: XP_022158196.1 (putative ribosome-inactivating protein, partial [Momordica charantia])

HSP 1 Score: 405.2 bits (1040), Expect = 5.1e-109
Identity = 201/202 (99.50%), Postives = 201/202 (99.50%), Query Frame = 0


Query: 1   MDHILQCHEENGGYKKHILEMFQRHPIQSNPSFENMSYSNSTLCCLLLAIILVAAPHTAE 60
           MDHILQCHEENGGYKKHILEMFQRHPIQSNPSFENMSYSNSTLCCLLLAIILVAAPHTAE
Sbjct: 1   MDHILQCHEENGGYKKHILEMFQRHPIQSNPSFENMSYSNSTLCCLLLAIILVAAPHTAE 60

Query: 61  GQREITFTTNINTYASELNAFRQAVRTTNKRFDYFLLPLRTAVSGTNRYREINLRNSRGV 120
           GQREITFTTNINTYASELNAFRQAVRTTNKRFDYFLLPLRTAVSGTNRYREINLRNS GV
Sbjct: 61  GQREITFTTNINTYASELNAFRQAVRTTNKRFDYFLLPLRTAVSGTNRYREINLRNSYGV 120

Query: 121 AVKLAIDIVNVYVLAYHTSNPSCRGSYFFNDADARAAMSFVFRNSGEQRVLPFDGSYGDL 180
           AVKLAIDIVNVYVLAYHTSNPSCRGSYFFNDADARAAMSFVFRNSGEQRVLPFDGSYGDL
Sbjct: 121 AVKLAIDIVNVYVLAYHTSNPSCRGSYFFNDADARAAMSFVFRNSGEQRVLPFDGSYGDL 180

Query: 181 AANGGSRRNTILGLSQLSDLLP 203
           AANGGSRRNTILGLSQLSDLLP
Sbjct: 181 AANGGSRRNTILGLSQLSDLLP 202

[Close]

BLAST of Moc07g04180 vs. NCBI nr
Match: XP_022158438.1 (ribosome-inactivating protein cucurmosin-like [Momordica charantia])

HSP 1 Score: 211.1 bits (536), Expect = 1.4e-50
Identity = 121/267 (45.32%), Postives = 168/267 (62.92%), Query Frame = 0


Query: 44  CCLLLAIILVAAPHTAEGQREITF-TTNINTYASELNAFRQAVRTTNKRFDYFLLPLRTA 103
           C L LAI L A  +T +GQR IT  TTNI++Y S LN FR A+R   K  +  L  LR  
Sbjct: 8   CLLFLAIFLAAPTYTVKGQRRITISTTNIDSYVSSLNTFRIALRDNKKVCNIPL--LRAN 67

Query: 104 VSGTNRYREINLRNSRGVAVKLAIDIVNVYVLAYHTSNPSCRGSYFFNDADARAAMSFVF 163
           V   NR+ EI++  S  + + LAID+VNVYVL Y+T NPSC   ++F+D  A  A  +VF
Sbjct: 68  VIDKNRFIEISIA-STNITIGLAIDLVNVYVLGYYTPNPSCSAYHYFSDPVAVRATQYVF 127

Query: 164 RNSGEQRVLPFDGSYGDLAANGGSRRNTILGLSQLSDLLPALYDCRRPGDTYDR--IATA 223
           +N  +Q+ LPFDG Y D    G +RR+  LGL+ L+ LLPALY+    G  Y +  +A A
Sbjct: 128 KNPTQQKSLPFDGGYPDFEKYGANRRSLDLGLTSLNHLLPALYEYGCTGTKYIQTDVAKA 187

Query: 224 LVLAIQTISEGARFKVVQDAVRRNSRPSEQVMDFQNSWQTISNAIQEANTRTGNFQNNIT 283
           LV+A+Q +SE ARF+++Q AV  N +P+ +V+  QNSW  +S A+Q A+   G F++ +T
Sbjct: 188 LVVAVQVVSEAARFRMIQIAVPNNHKPNAEVLSLQNSWSDLSEAVQRADCGNGRFKDKVT 247

Query: 284 LLNNQGVRVSVGNVDHNLVRGNVKLLL 308
           L + +G  V V N+    V GN+KLLL
Sbjct: 248 LNDEKGHPVDVWNISSKYVSGNLKLLL 271

[Close]

BLAST of Moc07g04180 vs. NCBI nr
Match: XP_022156706.1 (ribosome-inactivating protein bryodin I-like, partial [Momordica charantia])

HSP 1 Score: 191.0 bits (484), Expect = 1.5e-44
Identity = 113/268 (42.16%), Postives = 161/268 (60.07%), Query Frame = 0


Query: 46  LLLAIILVAAPHTAEGQREITFTTNINT-YASELNAFRQAVRTTNKRFDYFLLPLRTAVS 105
           LL+  I +A+P T EG      +T   T Y + LNAFR  +   NK+  Y +  LR  V+
Sbjct: 9   LLILTIFLASP-TVEGDVSFNLSTATKTSYGAFLNAFRADLPLENKK-PYNISLLRATVA 68

Query: 106 GTNRYREINLRNSRGVAVKLAIDIVNVYVLAYHTSNPSCRGSYFFNDADARAAMSFVFRN 165
           G  RY  I+L +  G A+ LAID+ NVY+L ++  +P C  S+FFND  + AA SFVF N
Sbjct: 69  GAARYTRIHLTDRSGRAITLAIDVTNVYLLGFY--SPQCNISHFFNDPVSIAAKSFVFTN 128

Query: 166 SGEQRVLPFDGSYGDLAANGGSRRNTILGLSQLSDLLPAL--YDCRRPGDTYDRIATALV 225
           S EQ+ LPFDGSY  LA  G +R + +LGL  L++ +P L  Y+C         +A A V
Sbjct: 129 SKEQKTLPFDGSYPQLAGVGANRSSLVLGLPSLNNFIPTLAGYNCPNTNYQPADVAKACV 188

Query: 226 LAIQTISEGARFKVVQDAVRRNSRPSEQVMDFQNSWQTISNAIQEANTRTGNFQNNITLL 285
           + IQT+SE ARF+ +QDAV  N  P+ +++  +N+W  +S  +Q A +  G F++N+TL 
Sbjct: 189 VTIQTMSEAARFQKIQDAVPNNLVPNAEILSLENNWGKLSKQVQLAESNGGRFKSNVTLQ 248

Query: 286 NNQGVRVSVGNVDHNLVRGNVKLLLTMQ 311
           +  G  V V NV+   VRGN++LLL  Q
Sbjct: 249 DPTGATVIVSNVNSPYVRGNIRLLLNQQ 272

[Close]

BLAST of Moc07g04180 vs. NCBI nr
Match: XP_022158190.1 (ribosome-inactivating protein bryodin I-like [Momordica charantia])

HSP 1 Score: 188.3 bits (477), Expect = 9.9e-44
Identity = 116/270 (42.96%), Postives = 166/270 (61.48%), Query Frame = 0


Query: 45  CLLLAIILVAAPHTAEGQREITFTTNINT-YASELNAFRQAVRTTNKRFDYFLLPLRTAV 104
           CLL+  + +AAP T EG      +    T YA+ LNA+R  +      FD  LLP+   V
Sbjct: 8   CLLILAVFLAAP-TVEGDVSFNLSGVTKTSYATFLNAYRADLPYKETVFDIILLPVN--V 67

Query: 105 SGTNRYREINLRNSRGVAVKLAIDIVNVYVLAYHTSNPSCRGSYFFNDADARAAMSFVFR 164
           +G++RY  I+L NS    ++LAID+ NVYVL ++T+   C  S+FFND+ A  A  FVF 
Sbjct: 68  TGSSRYTRIHLTNSYNQTIQLAIDVTNVYVLGFYTT--ECNLSHFFNDSVATEAKKFVFT 127

Query: 165 NSGEQRVLPFDGSYGDLAANGGSRRNTILGLSQLSDL-LPALYDCRRPGDTY--DRIATA 224
           +S +Q+ L FDGSYG LA  GG R++  LGL  L+ + +P LY    PG +Y    +A A
Sbjct: 128 DS-KQQTLSFDGSYGQLAGAGGRRQSLKLGLVDLNYVYIPTLYGYNCPGYSYKPSDVAKA 187

Query: 225 LVLAIQTISEGARFKVVQDAVRRNSRPSEQVMDFQNSWQTISNAIQEANTRTGNFQNNIT 284
           +V+ IQTISE ARF+ +Q  V+ NS P+ +V+  +N+W  +S  +++A+  TGNF+ N T
Sbjct: 188 MVVVIQTISEAARFQKIQKDVKYNSIPTAEVLSLENNWSKLSKLVKQADPTTGNFKVNFT 247

Query: 285 LLNNQGVRVSVGNVDHNLVRGNVKLLLTMQ 311
           L N  G  V V NV+   V+GN++LLL  Q
Sbjct: 248 LENATGTPVVVSNVNSPYVKGNIQLLLNKQ 271

[Close]

BLAST of Moc07g04180 vs. NCBI nr
Match: XP_022158442.1 (ribosome-inactivating protein momordin I-like [Momordica charantia])

HSP 1 Score: 172.9 bits (437), Expect = 4.3e-39
Identity = 90/204 (44.12%), Postives = 131/204 (64.22%), Query Frame = 0


Query: 111 EINLRNSRGVAVKLAIDIVNVYVLAYHTSNPSCRGSYFFNDADARAAMSFVFRNSGEQRV 170
           EI+L NS GV V+LAID++NVYVL Y+T +P+C GSYFF D  A  A  +VF +S  +  
Sbjct: 2   EISLTNSNGVTVRLAIDVMNVYVLGYYTPHPNCSGSYFFKDPVAERAKKYVFNSSHPKTT 61

Query: 171 LPFDGSYGDLAANGGSRRNTILGLSQLSDLLPALYDCRRPGDTYD--RIATALVLAIQTI 230
           L FDGSY  L  NG SR++ ILGL  LS L+ ALY+ + P   Y+   +A A ++AIQ I
Sbjct: 62  LQFDGSYRGLENNGASRKSQILGLPALSKLVQALYEYKCPNSNYNNTHVARAFMVAIQAI 121

Query: 231 SEGARFKVVQDAVRRNSRPSEQVMDFQNSWQTISNAIQEANTRTGNFQNNITLLNNQGVR 290
           SE ARF+ + DAV  + +P+ + +  +N+W  +S A+  A+  T  F++N+TL++     
Sbjct: 122 SEAARFQKIADAVPNDRKPTREALKLENNWSKLSKAVLNADPTTKEFKSNLTLVDANDKD 181

Query: 291 VSVGNVDHNLVRGNVKLLLTMQPR 313
           V+V N +   V+GN+ LLL  + R
Sbjct: 182 VNVSNANSPYVKGNLHLLLKPKDR 205

[Close]

BLAST of Moc07g04180 vs. ExPASy Swiss-Prot
Match: P24478 (Ribosome-inactivating protein karasurin-C OS=Trichosanthes kirilowii OX=3677 PE=1 SV=2)

HSP 1 Score: 126.3 bits (316), Expect = 6.0e-28
Identity = 88/268 (32.84%), Postives = 139/268 (51.87%), Query Frame = 0


Query: 46  LLLAIILVAAPHTAEGQREITFT-TNINTYASELNAFRQAVRTTNKRFDYFLLPLRTAVS 105
           LL+  + + AP   EG      +    ++Y   ++  R+A+    K +D  L  LR+ + 
Sbjct: 9   LLILTLFLTAP-AVEGDVSFRLSGATSSSYGVFISNLRKALPYERKLYDIPL--LRSTLP 68

Query: 106 GTNRYREINLRNSRGVAVKLAIDIVNVYVLAYHTSNPSCRGSYFFNDADARAAMSFVFRN 165
           G+ RY  I+L N     + +AID+ NVYV+ Y   +     SYFFN+A A  A  +VF++
Sbjct: 69  GSQRYALIHLTNYADETISVAIDVTNVYVMGYRAGDT----SYFFNEASATEAAKYVFKD 128

Query: 166 SGEQRVLPFDGSYGDL-AANGGSRRNTILGLSQLSDLLPALYDCRRPGDTYDRIATALVL 225
           +  +  LP+ G+Y  L  A G  R N  LGL  L   +  L+         +  A+AL++
Sbjct: 129 AKRKVTLPYSGNYERLQIAAGKIRENIPLGLPALDSAITTLFYYNA-----NSAASALMV 188

Query: 226 AIQTISEGARFKVVQD----AVRRNSRPSEQVMDFQNSWQTISNAIQEANTRTGNFQNNI 285
            IQ+ SE AR+K ++      V +   PS  ++  +NSW  +S  IQ A+T  G F+  +
Sbjct: 189 LIQSTSEAARYKFIEQQIGKRVDKTFLPSLAIISLENSWSALSKQIQIASTNNGQFETPV 248

Query: 286 TLLNNQGVRVSVGNVDHNLVRGNVKLLL 308
            L+N Q  RV++ NVD  +V  N+ LLL
Sbjct: 249 VLINAQNQRVTITNVDAGVVTSNIALLL 264

[Close]

BLAST of Moc07g04180 vs. ExPASy Swiss-Prot
Match: P09989 (Ribosome-inactivating protein alpha-trichosanthin OS=Trichosanthes kirilowii OX=3677 PE=1 SV=2)

HSP 1 Score: 126.3 bits (316), Expect = 6.0e-28
Identity = 86/270 (31.85%), Postives = 142/270 (52.59%), Query Frame = 0


Query: 43  LCCLLLAIILVAAPHTAEGQREITFTTNINTYASELNAFRQAVRTTNKRFDYFLLPLRTA 102
           L  L+L + L       +    ++  T+ ++Y   ++  R+A+    K +D  L  LR++
Sbjct: 7   LSLLILTLFLTTPAVEGDVSFRLSGATS-SSYGVFISNLRKALPNERKLYDIPL--LRSS 66

Query: 103 VSGTNRYREINLRNSRGVAVKLAIDIVNVYVLAYHTSNPSCRGSYFFNDADARAAMSFVF 162
           + G+ RY  I+L N     + +AID+ NVY++ Y   +     SYFFN+A A  A  +VF
Sbjct: 67  LPGSQRYALIHLTNYADETISVAIDVTNVYIMGYRAGDT----SYFFNEASATEAAKYVF 126

Query: 163 RNSGEQRVLPFDGSYGDL-AANGGSRRNTILGLSQLSDLLPALYDCRRPGDTYDRIATAL 222
           +++  +  LP+ G+Y  L  A G  R N  LGL  L   +  L+         +  A+AL
Sbjct: 127 KDAMRKVTLPYSGNYERLQTAAGKIRENIPLGLPALDSAITTLFYYNA-----NSAASAL 186

Query: 223 VLAIQTISEGARFKVVQD----AVRRNSRPSEQVMDFQNSWQTISNAIQEANTRTGNFQN 282
           ++ IQ+ SE AR+K ++      V +   PS  ++  +NSW  +S  IQ A+T  G F++
Sbjct: 187 MVLIQSTSEAARYKFIEQQIGKRVDKTFLPSLAIISLENSWSALSKQIQIASTNNGQFES 246

Query: 283 NITLLNNQGVRVSVGNVDHNLVRGNVKLLL 308
            + L+N Q  RV++ NVD  +V  N+ LLL
Sbjct: 247 PVVLINAQNQRVTITNVDAGVVTSNIALLL 264

[Close]

BLAST of Moc07g04180 vs. ExPASy Swiss-Prot
Match: P33185 (Ribosome-inactivating protein bryodin I OS=Bryonia dioica OX=3652 PE=1 SV=3)

HSP 1 Score: 120.9 bits (302), Expect = 2.5e-26
Identity = 84/268 (31.34%), Postives = 138/268 (51.49%), Query Frame = 0


Query: 46  LLLAIILVAAPHTAEGQREITFT-TNINTYASELNAFRQAVRTTNKRFDYFLLPLRTAVS 105
           LL+  I + +P T EG      +     +Y   +   R+A+    K ++  L  LR+++S
Sbjct: 9   LLILTIFLKSP-TVEGDVSFRLSGATTTSYGVFIKNLREALPYERKVYNIPL--LRSSIS 68

Query: 106 GTNRYREINLRNSRGVAVKLAIDIVNVYVLAYHTSNPSCRGSYFFNDADARAAMSFVFRN 165
           G+ RY  ++L N     + +A+D+ NVY++ Y   +     SYFFN+A A  A  FVF++
Sbjct: 69  GSGRYTLLHLTNYADETISVAVDVTNVYIMGYLAGDV----SYFFNEASATEAAKFVFKD 128

Query: 166 SGEQRVLPFDGSYGDL-AANGGSRRNTILGLSQLSDLLPALYDCRRPGDTYDRIATALVL 225
           + ++  LP+ G+Y  L  A G  R N  LGL  L   +  LY       T    A+AL++
Sbjct: 129 AKKKVTLPYSGNYERLQTAAGKIRENIPLGLPALDSAITTLYYY-----TASSAASALLV 188

Query: 226 AIQTISEGARFKVVQD----AVRRNSRPSEQVMDFQNSWQTISNAIQEANTRTGNFQNNI 285
            IQ+ +E AR+K ++      V +   PS   +  +N+W  +S  IQ A+T  G F++ +
Sbjct: 189 LIQSTAESARYKFIEQQIGKRVDKTFLPSLATISLENNWSALSKQIQIASTNNGQFESPV 248

Query: 286 TLLNNQGVRVSVGNVDHNLVRGNVKLLL 308
            L++    RVS+ N    +V  N+ LLL
Sbjct: 249 VLIDGNNQRVSITNASARVVTSNIALLL 264

[Close]

BLAST of Moc07g04180 vs. ExPASy Swiss-Prot
Match: Q00465 (Ribosome-inactivating protein luffin-alpha OS=Luffa aegyptiaca OX=3670 PE=1 SV=1)

HSP 1 Score: 116.7 bits (291), Expect = 4.8e-25
Identity = 81/269 (30.11%), Postives = 142/269 (52.79%), Query Frame = 0


Query: 46  LLLAIILVAAPHTAEGQREITFTTNINTYASELNAFRQAVRTTNKRFDYFLLPLRTAVSG 105
           L+LAI + A+   A+ +  ++ +++  +Y+  +   R+A+ +    ++  L  L ++ SG
Sbjct: 7   LILAIFVAASTVEADVRFSLSGSSS-TSYSKFIGDLRKALPSNGTVYNITL--LLSSASG 66

Query: 106 TNRYREINLRNSRGVAVKLAIDIVNVYVLAYHTSNPSCRGSYFFNDADARAAMSFVFRNS 165
            +RY  + L N  G A+ +A+D+ NVY++ Y  ++     SYFFN++DA+ A  +VF+ S
Sbjct: 67  ASRYTLMTLSNYDGKAITVAVDVTNVYIMGYLVNST----SYFFNESDAKLASQYVFKGS 126

Query: 166 GEQRVLPFDGSYGDLAANGGSRRNTI-LGLSQLSDLLPALYDCRRPGDTYD--RIATALV 225
                LP+ G+Y  L    G  R  I LG   L   +  L+        YD    A A +
Sbjct: 127 -TIVTLPYSGNYEKLQTAAGKIREKIPLGFPALDSAITTLFH-------YDSTAAAAAFL 186

Query: 226 LAIQTISEGARFKVVQ----DAVRRNSRPSEQVMDFQNSWQTISNAIQEANTRTGNFQNN 285
           + IQT +E +RFK ++    + + +N  PS   +  +N W  +S  IQ A T  G F+  
Sbjct: 187 VIIQTTAEASRFKYIEGQIIERISKNQVPSLATISLENEWSALSKQIQLAQTNNGTFKTP 246

Query: 286 ITLLNNQGVRVSVGNVDHNLVRGNVKLLL 308
           + + +++G RV + NV   +V  N++LLL
Sbjct: 247 VVITDDKGQRVEITNVTSKVVTKNIQLLL 260

[Close]

BLAST of Moc07g04180 vs. ExPASy Swiss-Prot
Match: P84530 (Ribosome-inactivating protein luffaculin 1 OS=Luffa acutangula OX=56866 PE=1 SV=2)

HSP 1 Score: 115.9 bits (289), Expect = 8.2e-25
Identity = 78/242 (32.23%), Postives = 125/242 (51.65%), Query Frame = 0


Query: 73  TYASELNAFRQAVRTTNKRFDYFLLPLRTAVSGTNRYREINLRNSRGVAVKLAIDIVNVY 132
           +Y+  + A R+A+ +    ++  L  L ++ SG +RY  + L N  G A+ +AID+ NVY
Sbjct: 13  SYSKFIGALRKALPSNGTVYNITL--LLSSASGASRYTLMKLSNYDGKAITVAIDVTNVY 72

Query: 133 VLAYHTSNPSCRGSYFFNDADARAAMSFVFRNSGEQRVLPFDGSYGDLAANGGSRRNTI- 192
           ++ Y  ++     SYFFN++DA+ A  +VF  S     LP+ G+Y  L    G  R  I 
Sbjct: 73  IMGYLVNST----SYFFNESDAKLASQYVFAGS-TIVTLPYSGNYEKLQTAAGKIREKIP 132

Query: 193 LGLSQLSDLLPALYDCRRPGDTYD--RIATALVLAIQTISEGARFKVVQD----AVRRNS 252
           LG   L   +  L+        YD    A A ++ IQT +E +RFK ++      + +N 
Sbjct: 133 LGFPALDSAITTLFH-------YDSTAAAAAFLVIIQTTAESSRFKYIEGQIIMRISKNG 192

Query: 253 RPSEQVMDFQNSWQTISNAIQEANTRTGNFQNNITLLNNQGVRVSVGNVDHNLVRGNVKL 308
            PS   +  +N W  +S  IQ A T  G F+  + +++  G RV +GNV   +V  N++L
Sbjct: 193 VPSLATISLENEWSALSKQIQLAQTNNGTFKTPVVIMDAGGQRVEIGNVGSKVVTKNIQL 240

[Close]

BLAST of Moc07g04180 vs. ExPASy TrEMBL
Match: A0A6J1E098 (rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111024731 PE=3 SV=1)

HSP 1 Score: 405.2 bits (1040), Expect = 2.5e-109
Identity = 201/202 (99.50%), Postives = 201/202 (99.50%), Query Frame = 0


Query: 1   MDHILQCHEENGGYKKHILEMFQRHPIQSNPSFENMSYSNSTLCCLLLAIILVAAPHTAE 60
           MDHILQCHEENGGYKKHILEMFQRHPIQSNPSFENMSYSNSTLCCLLLAIILVAAPHTAE
Sbjct: 1   MDHILQCHEENGGYKKHILEMFQRHPIQSNPSFENMSYSNSTLCCLLLAIILVAAPHTAE 60

Query: 61  GQREITFTTNINTYASELNAFRQAVRTTNKRFDYFLLPLRTAVSGTNRYREINLRNSRGV 120
           GQREITFTTNINTYASELNAFRQAVRTTNKRFDYFLLPLRTAVSGTNRYREINLRNS GV
Sbjct: 61  GQREITFTTNINTYASELNAFRQAVRTTNKRFDYFLLPLRTAVSGTNRYREINLRNSYGV 120

Query: 121 AVKLAIDIVNVYVLAYHTSNPSCRGSYFFNDADARAAMSFVFRNSGEQRVLPFDGSYGDL 180
           AVKLAIDIVNVYVLAYHTSNPSCRGSYFFNDADARAAMSFVFRNSGEQRVLPFDGSYGDL
Sbjct: 121 AVKLAIDIVNVYVLAYHTSNPSCRGSYFFNDADARAAMSFVFRNSGEQRVLPFDGSYGDL 180

Query: 181 AANGGSRRNTILGLSQLSDLLP 203
           AANGGSRRNTILGLSQLSDLLP
Sbjct: 181 AANGGSRRNTILGLSQLSDLLP 202

[Close]

BLAST of Moc07g04180 vs. ExPASy TrEMBL
Match: A0A6J1DW33 (rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111024927 PE=3 SV=1)

HSP 1 Score: 211.1 bits (536), Expect = 6.9e-51
Identity = 121/267 (45.32%), Postives = 168/267 (62.92%), Query Frame = 0


Query: 44  CCLLLAIILVAAPHTAEGQREITF-TTNINTYASELNAFRQAVRTTNKRFDYFLLPLRTA 103
           C L LAI L A  +T +GQR IT  TTNI++Y S LN FR A+R   K  +  L  LR  
Sbjct: 8   CLLFLAIFLAAPTYTVKGQRRITISTTNIDSYVSSLNTFRIALRDNKKVCNIPL--LRAN 67

Query: 104 VSGTNRYREINLRNSRGVAVKLAIDIVNVYVLAYHTSNPSCRGSYFFNDADARAAMSFVF 163
           V   NR+ EI++  S  + + LAID+VNVYVL Y+T NPSC   ++F+D  A  A  +VF
Sbjct: 68  VIDKNRFIEISIA-STNITIGLAIDLVNVYVLGYYTPNPSCSAYHYFSDPVAVRATQYVF 127

Query: 164 RNSGEQRVLPFDGSYGDLAANGGSRRNTILGLSQLSDLLPALYDCRRPGDTYDR--IATA 223
           +N  +Q+ LPFDG Y D    G +RR+  LGL+ L+ LLPALY+    G  Y +  +A A
Sbjct: 128 KNPTQQKSLPFDGGYPDFEKYGANRRSLDLGLTSLNHLLPALYEYGCTGTKYIQTDVAKA 187

Query: 224 LVLAIQTISEGARFKVVQDAVRRNSRPSEQVMDFQNSWQTISNAIQEANTRTGNFQNNIT 283
           LV+A+Q +SE ARF+++Q AV  N +P+ +V+  QNSW  +S A+Q A+   G F++ +T
Sbjct: 188 LVVAVQVVSEAARFRMIQIAVPNNHKPNAEVLSLQNSWSDLSEAVQRADCGNGRFKDKVT 247

Query: 284 LLNNQGVRVSVGNVDHNLVRGNVKLLL 308
           L + +G  V V N+    V GN+KLLL
Sbjct: 248 LNDEKGHPVDVWNISSKYVSGNLKLLL 271

[Close]

BLAST of Moc07g04180 vs. ExPASy TrEMBL
Match: A0A6J1DVR9 (rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111023550 PE=3 SV=1)

HSP 1 Score: 191.0 bits (484), Expect = 7.4e-45
Identity = 113/268 (42.16%), Postives = 161/268 (60.07%), Query Frame = 0


Query: 46  LLLAIILVAAPHTAEGQREITFTTNINT-YASELNAFRQAVRTTNKRFDYFLLPLRTAVS 105
           LL+  I +A+P T EG      +T   T Y + LNAFR  +   NK+  Y +  LR  V+
Sbjct: 9   LLILTIFLASP-TVEGDVSFNLSTATKTSYGAFLNAFRADLPLENKK-PYNISLLRATVA 68

Query: 106 GTNRYREINLRNSRGVAVKLAIDIVNVYVLAYHTSNPSCRGSYFFNDADARAAMSFVFRN 165
           G  RY  I+L +  G A+ LAID+ NVY+L ++  +P C  S+FFND  + AA SFVF N
Sbjct: 69  GAARYTRIHLTDRSGRAITLAIDVTNVYLLGFY--SPQCNISHFFNDPVSIAAKSFVFTN 128

Query: 166 SGEQRVLPFDGSYGDLAANGGSRRNTILGLSQLSDLLPAL--YDCRRPGDTYDRIATALV 225
           S EQ+ LPFDGSY  LA  G +R + +LGL  L++ +P L  Y+C         +A A V
Sbjct: 129 SKEQKTLPFDGSYPQLAGVGANRSSLVLGLPSLNNFIPTLAGYNCPNTNYQPADVAKACV 188

Query: 226 LAIQTISEGARFKVVQDAVRRNSRPSEQVMDFQNSWQTISNAIQEANTRTGNFQNNITLL 285
           + IQT+SE ARF+ +QDAV  N  P+ +++  +N+W  +S  +Q A +  G F++N+TL 
Sbjct: 189 VTIQTMSEAARFQKIQDAVPNNLVPNAEILSLENNWGKLSKQVQLAESNGGRFKSNVTLQ 248

Query: 286 NNQGVRVSVGNVDHNLVRGNVKLLLTMQ 311
           +  G  V V NV+   VRGN++LLL  Q
Sbjct: 249 DPTGATVIVSNVNSPYVRGNIRLLLNQQ 272

[Close]

BLAST of Moc07g04180 vs. ExPASy TrEMBL
Match: A0A6J1DV53 (rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111024724 PE=3 SV=1)

HSP 1 Score: 188.3 bits (477), Expect = 4.8e-44
Identity = 116/270 (42.96%), Postives = 166/270 (61.48%), Query Frame = 0


Query: 45  CLLLAIILVAAPHTAEGQREITFTTNINT-YASELNAFRQAVRTTNKRFDYFLLPLRTAV 104
           CLL+  + +AAP T EG      +    T YA+ LNA+R  +      FD  LLP+   V
Sbjct: 8   CLLILAVFLAAP-TVEGDVSFNLSGVTKTSYATFLNAYRADLPYKETVFDIILLPVN--V 67

Query: 105 SGTNRYREINLRNSRGVAVKLAIDIVNVYVLAYHTSNPSCRGSYFFNDADARAAMSFVFR 164
           +G++RY  I+L NS    ++LAID+ NVYVL ++T+   C  S+FFND+ A  A  FVF 
Sbjct: 68  TGSSRYTRIHLTNSYNQTIQLAIDVTNVYVLGFYTT--ECNLSHFFNDSVATEAKKFVFT 127

Query: 165 NSGEQRVLPFDGSYGDLAANGGSRRNTILGLSQLSDL-LPALYDCRRPGDTY--DRIATA 224
           +S +Q+ L FDGSYG LA  GG R++  LGL  L+ + +P LY    PG +Y    +A A
Sbjct: 128 DS-KQQTLSFDGSYGQLAGAGGRRQSLKLGLVDLNYVYIPTLYGYNCPGYSYKPSDVAKA 187

Query: 225 LVLAIQTISEGARFKVVQDAVRRNSRPSEQVMDFQNSWQTISNAIQEANTRTGNFQNNIT 284
           +V+ IQTISE ARF+ +Q  V+ NS P+ +V+  +N+W  +S  +++A+  TGNF+ N T
Sbjct: 188 MVVVIQTISEAARFQKIQKDVKYNSIPTAEVLSLENNWSKLSKLVKQADPTTGNFKVNFT 247

Query: 285 LLNNQGVRVSVGNVDHNLVRGNVKLLLTMQ 311
           L N  G  V V NV+   V+GN++LLL  Q
Sbjct: 248 LENATGTPVVVSNVNSPYVKGNIQLLLNKQ 271

[Close]

BLAST of Moc07g04180 vs. ExPASy TrEMBL
Match: A0A6J1DX83 (rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111024930 PE=3 SV=1)

HSP 1 Score: 172.9 bits (437), Expect = 2.1e-39
Identity = 90/204 (44.12%), Postives = 131/204 (64.22%), Query Frame = 0


Query: 111 EINLRNSRGVAVKLAIDIVNVYVLAYHTSNPSCRGSYFFNDADARAAMSFVFRNSGEQRV 170
           EI+L NS GV V+LAID++NVYVL Y+T +P+C GSYFF D  A  A  +VF +S  +  
Sbjct: 2   EISLTNSNGVTVRLAIDVMNVYVLGYYTPHPNCSGSYFFKDPVAERAKKYVFNSSHPKTT 61

Query: 171 LPFDGSYGDLAANGGSRRNTILGLSQLSDLLPALYDCRRPGDTYD--RIATALVLAIQTI 230
           L FDGSY  L  NG SR++ ILGL  LS L+ ALY+ + P   Y+   +A A ++AIQ I
Sbjct: 62  LQFDGSYRGLENNGASRKSQILGLPALSKLVQALYEYKCPNSNYNNTHVARAFMVAIQAI 121

Query: 231 SEGARFKVVQDAVRRNSRPSEQVMDFQNSWQTISNAIQEANTRTGNFQNNITLLNNQGVR 290
           SE ARF+ + DAV  + +P+ + +  +N+W  +S A+  A+  T  F++N+TL++     
Sbjct: 122 SEAARFQKIADAVPNDRKPTREALKLENNWSKLSKAVLNADPTTKEFKSNLTLVDANDKD 181

Query: 291 VSVGNVDHNLVRGNVKLLLTMQPR 313
           V+V N +   V+GN+ LLL  + R
Sbjct: 182 VNVSNANSPYVKGNLHLLLKPKDR 205

The following BLAST results are available for this feature:

BLAST of Moc07g04180 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022158196.1	5.1e-109	99.50	putative ribosome-inactivating protein, partial [Momordica charantia]	[more]
XP_022158438.1	1.4e-50	45.32	ribosome-inactivating protein cucurmosin-like [Momordica charantia]	[more]
XP_022156706.1	1.5e-44	42.16	ribosome-inactivating protein bryodin I-like, partial [Momordica charantia]	[more]
XP_022158190.1	9.9e-44	42.96	ribosome-inactivating protein bryodin I-like [Momordica charantia]	[more]
XP_022158442.1	4.3e-39	44.12	ribosome-inactivating protein momordin I-like [Momordica charantia]	[more]

BLAST of Moc07g04180 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P24478	6.0e-28	32.84	Ribosome-inactivating protein karasurin-C OS=Trichosanthes kirilowii OX=3677 PE=...	[more]
P09989	6.0e-28	31.85	Ribosome-inactivating protein alpha-trichosanthin OS=Trichosanthes kirilowii OX=...	[more]
P33185	2.5e-26	31.34	Ribosome-inactivating protein bryodin I OS=Bryonia dioica OX=3652 PE=1 SV=3	[more]
Q00465	4.8e-25	30.11	Ribosome-inactivating protein luffin-alpha OS=Luffa aegyptiaca OX=3670 PE=1 SV=1	[more]
P84530	8.2e-25	32.23	Ribosome-inactivating protein luffaculin 1 OS=Luffa acutangula OX=56866 PE=1 SV=...	[more]

BLAST of Moc07g04180 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1E098	2.5e-109	99.50	rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111024731 PE=3 SV=1	[more]
A0A6J1DW33	6.9e-51	45.32	rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111024927 PE=3 SV=1	[more]
A0A6J1DVR9	7.4e-45	42.16	rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111023550 PE=3 SV=1	[more]
A0A6J1DV53	4.8e-44	42.96	rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111024724 PE=3 SV=1	[more]
A0A6J1DX83	2.1e-39	44.12	rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111024930 PE=3 SV=1	[more]

BLAST of Moc07g04180 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR017989	Ribosome-inactivating protein type 1/2	PRINTS	PR00396	SHIGARICIN	coord: 249..266 score: 30.41 coord: 221..241 score: 41.48 coord: 187..201 score: 32.11 coord: 72..85 score: 27.82 coord: 121..136 score: 45.07
IPR016138	Ribosome-inactivating protein, subdomain 1	GENE3D	3.40.420.10	Ricin (A subunit), domain 1	coord: 63..233 e-value: 3.2E-30 score: 107.2
IPR016139	Ribosome-inactivating protein, subdomain 2	GENE3D	4.10.470.10	Ricin (A Subunit), domain 2	coord: 234..310 e-value: 5.9E-16 score: 60.1
IPR001574	Ribosome-inactivating protein	PFAM	PF00161	RIP	coord: 72..266 e-value: 2.6E-24 score: 85.9
IPR001574	Ribosome-inactivating protein	PANTHER	PTHR33453	FAMILY NOT NAMED	coord: 68..308
None	No IPR available	PANTHER	PTHR33453:SF14	RRNA N-GLYCOSIDASE	coord: 68..308
IPR017988	Ribosome-inactivating protein conserved site	PROSITE	PS00275	SHIGA_RICIN	coord: 225..241
IPR036041	Ribosome-inactivating protein superfamily	SUPERFAMILY	56371	Ribosome inactivating proteins (RIP)	coord: 66..309

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc07g04180.1	Moc07g04180.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006952	defense response
biological_process	GO:0035821	modulation of process of other organism
biological_process	GO:0017148	negative regulation of translation
molecular_function	GO:0030598	rRNA N-glycosylase activity
molecular_function	GO:0090729	toxin activity